LOCUS       NZ_CP064270            17417 bp    DNA     circular CON 17-APR-2024
DEFINITION  Priestia megaterium strain N447-3 chromosome, complete genome.
ACCESSION   NZ_CP064270
VERSION     NZ_CP064270.1
KEYWORDS    RefSeq.
SOURCE      Priestia megaterium (Bacillus megaterium)
  ORGANISM  Priestia megaterium
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Priestia.
REFERENCE   1  (bases 1 to 5080837)
  AUTHORS   Wang,C., Jia,Z., Zhao,J., Wang,X., Liu,Z. and Ji,F.
  TITLE     Comparative Genome Analysis of key enzyme genes in ammonia nitrogen
            Metabolism Pathway of bacteria from different sources
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5080837)
  AUTHORS   Wang,C., Jia,Z., Zhao,J., Wang,X., Liu,Z. and Ji,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-OCT-2020) Guangdong Key Laboratory of Animal
            Conservation and Resource Utilization, Guangdong Public Laboratory
            of Wild Animal Conservation and Utilization, Institute of Zoology,
            Guangdong Academy of Science, Microbial Pathogenic Ecology Group,
            105 Xingang West Road, Guangzhou 510260, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. V1.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 331.67x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022811865.1-RS_2024_04_17
            Annotation Date                   :: 04/17/2024 10:21:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,644
            CDSs (total)                      :: 5,465
            Genes (coding)                    :: 5,410
            CDSs (with protein)               :: 5,410
            Genes (RNA)                       :: 179
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 131
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 17 of 55
            Pseudo Genes (incomplete)         :: 32 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 5 of 55
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 08:04:25
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2497289
            Orig. end                         :: 2514706
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP064270.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..17417
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="phosphonate"
                     /region_number="4"
                     /rules="(phosphonates and minimum(1, [2-Hacid_dh_C, Aldedh,
                     Aminotran_1_2, Aminotran_5, AurF, CTP_transf_like, DUF4992,
                     Fe-ADH, HMGL-like, Metallophos_2, NTP_transf_3,
                     NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C, TPP_enzyme_N,
                     phosphonates]) and not (phosphonatesHydrolase or
                     phosphonatesMeIsocitrateLyase))"
                     /tool="antismash"
     cand_cluster    1..17417
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /kind="single"
                     /product="phosphonate"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..17417
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:12417]"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..12417
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
     gene            complement(368..1324)
                     /locus_tag="IQ863_RS12835"
     CDS             complement(368..1324)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1083: oxidoreductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013081827.1"
                     /locus_tag="IQ863_RS12835"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="LLM class oxidoreductase"
                     /protein_id="WP_014461497.1"
                     /transl_table=11
                     /translation="MTTFQDHKAYRRMYKENELTLGLHMPLENYQYATPTMEKQVELTQ
                     LAEQYGFTSLWFRDVLLQDPSFGDPATGQIYDMLIYLTYLASQTKEIALSTSAIVLPLR
                     HPLRIAKEMATIDQLFPERLIMGVSSGDRQADFRGLGIPHEERGKRFVEAFHYLEEALN
                     KEFPSIQSALGSIQGANVVPKPTKRIPTMITGYSQQNMEWFAQYGDGWMYYPRHPHFQA
                     EAITQWRKLVDSYHPGVFKPFTQPMHLDLAEDPNEMPTPIRLGFRAGRNTLIELLSIYK
                     EIGVNHLFFALFDSKRPAEEVIHELGEEVLPHFPGHM"
     gene            complement(1382..2581)
                     /locus_tag="IQ863_RS12840"
     CDS             complement(1382..2581)
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1184: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014461498.1"
                     /locus_tag="IQ863_RS12840"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MFS transporter"
                     /protein_id="WP_014461498.1"
                     /transl_table=11
                     /translation="MNTLSQTQQSKKSGALPLLALAISAFGIGTTEFVPVGLLSSLADD
                     LNISITLAGLLISGYAMGVAFGAPVLTALTSKMSRKSLLMLLMVIFIVGNSVAALSTSF
                     GLLLIARIITAFSHGIFFSIGATIAADLVPEDKRASAIALMFTGLTVATVTGVPLGTFI
                     GQAFGWRATFWGVALLGVIAIIASALLVPKNLKEAPPAKFSDQLKILKNGPLLLSFAIT
                     ALGYGGTFVAYTYLAPILEKLTGYAPSAVSILLLVYGVAVAIGNTIGGKAANKNPLKAL
                     FWMFLIQAVILVILSFTASFKVAGTITIFAMGLLAFMNVPGLQVLVVNLAEKYVPSAVN
                     VASALNIAAFNVGIAIGSFVGGLIVDSIGLVHTPWIGGVMVLGAVLLTAWSRSIEAKAK
                     "
     gene            2911..3279
                     /locus_tag="IQ863_RS12845"
     CDS             2911..3279
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1167:
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014461499.1"
                     /locus_tag="IQ863_RS12845"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_014461499.1"
                     /transl_table=11
                     /translation="MKKYNIPVEAALEVIGGKWKVVILCHLIKGTKRPGELKRIMPRVT
                     QKVLTQQLRELEADGIIVRKIYNEMPPRVEYSLTDYGWSLKSILDSLCSWGEHHIEKTY
                     PDKEAVLMQPVEEVTVSN"
     misc_feature    complement(3286..3301)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 20.77"
                     /tool="antismash"
     gene            3363..3542
                     /locus_tag="IQ863_RS12850"
     CDS             3363..3542
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002015431.1"
                     /locus_tag="IQ863_RS12850"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_014461500.1"
                     /transl_table=11
                     /translation="MTEQLKKKISLQDAIKQQLANKKNQSSNKAKNGSTNQATKKLTSQ
                     QTKKTNNQRKRTGV"
     gene            3542..3744
                     /locus_tag="IQ863_RS12855"
                     /pseudo=""
     CDS             3542..3744
                     /GO_component="GO:0005575 - cellular_component [Evidence
                     IEA]"
                     /GO_function="GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /GO_process="GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013055498.1"
                     /locus_tag="IQ863_RS12855"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="YwbE family protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MNGQNRKDIAPGTKVDIVLKADQRSGKLTHGTVKDILTNSSFHPH
                     GIKVRLTDGQDKSDV"
     gene            4607..4771
                     /locus_tag="IQ863_RS12860"
     CDS             4607..4771
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014461503.1"
                     /locus_tag="IQ863_RS12860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="aspartyl-phosphate phosphatase Spo0E family
                     protein"
                     /protein_id="WP_014461503.1"
                     /transl_table=11
                     /translation="MKQPQLEKEIRALQSDIYQLAKKTSSYSHGEILKLSQKLDQKIVS
                     YQKLFNHTK"
     misc_feature    4887..4901
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 22.86"
                     /tool="antismash"
     gene            5001..6677
                     /gene="alsS"
                     /locus_tag="IQ863_RS12865"
     CDS             5001..6677
                     /EC_number="2.2.1.6"
                     /GO_function="GO:0000287 - magnesium ion binding [Evidence
                     IEA]"
                     /GO_function="GO:0003984 - acetolactate synthase activity
                     [Evidence IEA]"
                     /GO_function="GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /GO_process="GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="alsS"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_N"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1055: pyruvate oxidase/decarboxylase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013081825.1"
                     /locus_tag="IQ863_RS12865"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="acetolactate synthase AlsS"
                     /protein_id="WP_014461504.1"
                     /sec_met_domain="TPP_enzyme_N (E-value: 4.2e-51, bitscore:
                     171.2, seeds: 75, tool: rule-based-clusters)"
                     /sec_met_domain="TPP_enzyme_C (E-value: 2.8e-43, bitscore:
                     145.6, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSNADTTETKKGADLLVESLASQNVPYIFGIPGAKIDGVFDVLKE
                     KGPEVIVCRHEQNAAFMAAAMGRLTGTPGVCLVTSGPGAANLATGLVTATSEGDPVVAL
                     AGAVPRADRLKKTHQSMDNEGLFKSIAKFSVEVEDAANVPEVITDAFREAVSSPSGASF
                     VSLPQDVMDSETSASTLSVLKTPTIGAANLDAVAQAIHTIRTAKLPVLLLGMRASKSKA
                     VESIRQLIEEHDLPVVETFQGAGAVSRELETHFFGRVGLFRNQPGDVLLKHADVVLTIG
                     YDPIEYPPSLWNSESQRSIIHLDDVQAQADHHYQPEIELVGDIPSTIDILTDKLPHLIR
                     SKESASFLKTLKSKLEQPDDQYEFSNDTHLHPLQLIEILQNFVEDDTTITCDVGSHYIW
                     MARYFRSYEPQHLLFSNGMQTLGVALPWAISAALVRPHKKVLSMSGDGGFLFSAMELET
                     AVRLNLPIVHIIWNDQTYDMVGFQQQIKYGRKSAVEFGSVDFVTLAESFGAKGFRVNSP
                     EEFSDTLASAFREQCPVIIDVPVDYKDNEKLNTQLLPDQIN"
     gene            6694..7407
                     /gene="budA"
                     /locus_tag="IQ863_RS12870"
     CDS             6694..7407
                     /EC_number="4.1.1.5"
                     /GO_function="GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006113 - fermentation [Evidence IEA]"
                     /GO_process="GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="budA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000215002.1"
                     /locus_tag="IQ863_RS12870"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="acetolactate decarboxylase"
                     /protein_id="WP_014461505.1"
                     /transl_table=11
                     /translation="MSNEEKLTIYQTSTMIALLDGVFDGVVTYKDLAKKGDFGIGTFNQ
                     LDGEMIAFDGEFYHIADGKAAPISEDAKTPFAIMTTFYEDISYRVEKEMNREQLETLMN
                     KLIPSPNLFYAVRIDGKFKEVKTRTVAKQEKPYPSMVDAAANQPTFTSSDVNGTLAGFY
                     TPEYSSGIGVPGYHLHFIDEYKKEGGHVLDVSVQDVTIQICKKTKLNLNLSETSEFLNA
                     NLEDHDVEEEIATAE"
     gene            complement(7515..7937)
                     /locus_tag="IQ863_RS12875"
     CDS             complement(7515..7937)
                     /EC_number="3.6.-.-"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014461506.1"
                     /locus_tag="IQ863_RS12875"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NUDIX hydrolase"
                     /protein_id="WP_014461506.1"
                     /transl_table=11
                     /translation="MNQKLAVAVMVVNQDHHVLLVKNHRRGWEFPGGFVEADESITDAG
                     IREVKEESGIVIEVTNFLGVEQDVKRSTTVILLKGKAISGEISVSSETQDVGYFTFEEA
                     KGIIKLDNFKDRMTRCLNETEIPFFYVLKDTDSFYV"
     gene            complement(7943..9784)
                     /locus_tag="IQ863_RS12880"
     CDS             complement(7943..9784)
                     /EC_number="2.6.1.37"
                     /GO_function="GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_5 (266-541). E-value: 3.5e-30.
                     Score: 97.0. Matches aSDomain:
                     nrpspksdomains_IQ863_RS12880_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: NTP_transf_3"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013055494.1"
                     /locus_tag="IQ863_RS12880"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="2-aminoethylphosphonate aminotransferase"
                     /protein_id="WP_014461507.1"
                     /sec_met_domain="NTP_transf_3 (E-value: 1.4e-10, bitscore:
                     40.0, seeds: 297, tool: rule-based-clusters)"
                     /sec_met_domain="Aminotran_5 (E-value: 1.9e-24, bitscore:
                     84.6, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIKTAVILAAGMGSRIRKRAGNRPKGFLMIDEKSIIEHSISKLIE
                     AGVKTIFIGTGYMKEEYEKLMVRYPQIKCVYNSRYETTGSLFTLYQFKSYIKEDFLLLE
                     SDVIYEKSALKTLINHSRSDVILASKLNDAGDEVYIEADENNNLKNMSKQKEELNSIYA
                     ELVGLTKLSYATFQRLCDEANTLFQLNQAIDYEYGLVKISEKANVYVHKLDNLAWCEVD
                     DEDHWARATTIVYPIIKAREGIPHAVKRNILLNPGPATTTDTVKYAQIVEDICPREKEF
                     GETMEFISAELTKFVGNSDKYTTVLFGGSGTAAVESILSSVIDNGTVVIINNGPYGERM
                     CKIAEIYGLDYAEYKSSAERAIDMNDLEVFIKKISANVSYLALVHCETSTGLLNDIEQI
                     GKLCAVENIEMIVDAMSSYAAVPIDMQKMNISYLAASANKNLQGMAGVSFVIANKERLE
                     KTKKMKPRNLYLHLYDQYRYFQMNKQMRFTPPVQTMYALKQAIIETQWEGIERRYERYS
                     KSWTTLTDGITRLGLTYLVPETHHSKIITSILEPSYQNYNFDIMHDFFYKQGFTIYPGK
                     IDKLETFRIANIGNITYRDIERFLHLLEQYLNTLKGE"
     aSDomain        complement(8162..8986)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IQ863_RS12880_Aminotran_5.1"
                     /evalue="3.50E-30"
                     /label="IQ863_RS12880_Aminotran_5.1"
                     /locus_tag="IQ863_RS12880"
                     /protein_end="541"
                     /protein_start="266"
                     /score="97.0"
                     /tool="antismash"
                     /translation="YAQIVEDICPREKEFGETMEFISAELTKFVGNSDKYTTVLFGGSG
                     TAAVESILSSVIDNGTVVIINNGPYGERMCKIAEIYGLDYAEYKSSAERAIDMNDLEVF
                     IKKISANVSYLALVHCETSTGLLNDIEQIGKLCAVENIEMIVDAMSSYAAVPIDMQKMN
                     ISYLAASANKNLQGMAGVSFVIANKERLEKTKKMKPRNLYLHLYDQYRYFQMNKQMRFT
                     PPVQTMYALKQAIIETQWEGIERRYERYSKSWTTLTDGITRLGLTYLVPETHH"
     gene            complement(9777..10907)
                     /gene="aepY"
                     /locus_tag="IQ863_RS12885"
     CDS             complement(9777..10907)
                     /EC_number="4.1.1.82"
                     /GO_function="GO:0033980 - phosphonopyruvate decarboxylase
                     activity [Evidence IEA]"
                     /GO_process="GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="aepY"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_N"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1055: pyruvate oxidase/decarboxylase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013081821.1"
                     /locus_tag="IQ863_RS12885"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="phosphonopyruvate decarboxylase"
                     /protein_id="WP_014461508.1"
                     /sec_met_domain="TPP_enzyme_N (E-value: 3.4e-09, bitscore:
                     34.8, seeds: 75, tool: rule-based-clusters)"
                     /sec_met_domain="TPP_enzyme_C (E-value: 1.6e-13, bitscore:
                     48.9, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MISTSLFGNQLKKLGFQFYSGVPCSYLKPLINYAINECEYVAAAN
                     EGEAVAIAAGASIAGKKAVVLMQNSGLANAVSPLTSLIHPFKIPVLGFVSFRGEPGFPD
                     EPQHELMGEITPALLNTMKIKWEYLSFDFTEVKNQLRRANQAIENNESYFFIVKKGTFE
                     SVKLSEKSMKNVTPKMKSEQITTDELPSRYEALQVVNSIKDNDTIQLAATGKTGRELYE
                     IEDAKNNFYMVGSMGCVSSIGLGLALSRNEKNIITIDGDGALLMRMGNLATNGYYCPEN
                     LLHILLDNQTHDSTGGQHTVSHNINFVDIASSCGYQNSIHVHNLNELEMYIKQWKNDKK
                     LTFLYLKIAKGSKKNLGRPEMKPYEVRERLQVFLDD"
     gene            complement(10900..12417)
                     /gene="aepX"
                     /locus_tag="IQ863_RS12890"
     CDS             complement(10900..12417)
                     /EC_number="5.4.2.9"
                     /GO_function="GO:0050188 - phosphoenolpyruvate mutase
                     activity [Evidence IEA]"
                     /GO_process="GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene="aepX"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: phosphonates"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: PEP_mutase"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) phosphonates-like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1231: carboxyvinyl-carboxyphosphonate"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013055492.1"
                     /locus_tag="IQ863_RS12890"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="phosphoenolpyruvate mutase"
                     /protein_id="WP_014461509.1"
                     /sec_met_domain="phosphonates-like (E-value: 1.9e-137,
                     bitscore: 455.0, seeds: 13, tool: rule-based-clusters)"
                     /sec_met_domain="phosphonates (E-value: 4.7e-126, bitscore:
                     418.0, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="PEP_mutase (E-value: 7.2e-63, bitscore:
                     210.5, seeds: 69, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKTKQLRELIESKQLEFIMEAHSALSAKIVEEAGFKGIWASGLS
                     LSAALGVRDNNEASWTQVLDILEFMSDATSVPILLDGDTGYGNFNNARRLVKKLEQRDI
                     PGVCIEDKLFPKTNSFIKGEAQPLADINEFCGKIKAMKDSQNDEDFVVVSRVEAFIAGW
                     GLKEALKRAEAYRQAGTDAVLIHSKRSDMTEIEAFMKEWGNRLPVIIVPTKYYSVPTDK
                     FSDLGISLSIWANHNLRSSITAMQKTSAQIYYDKSLINVEKNVSPLEEVFRLQKAEELD
                     MAEKLYLSSANHSINAMILVKKSANDVLITEQMNQLKKVGVTNSIKVDSNKAMEDKESP
                     NQGELYDLYAARDKLGTATLISDSNIVYKTHVFEELIHESGDITIVAGADYDLKGDQTS
                     YVTAEFPYSKKLYSKTVDLIQMSSSPNSKNIHGEFIGLWKVSGKGTEVVKKVLEELAQR
                     EDFAQLTFSDFFNYINLIHPVAVKYTMGSWIDTETYIKLQKDGGKND"
     gene            complement(12431..13840)
                     /gene="murF"
                     /locus_tag="IQ863_RS12895"
     CDS             complement(12431..13840)
                     /EC_number="6.3.2.10"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA]"
                     /GO_process="GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene="murF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013055491.1"
                     /locus_tag="IQ863_RS12895"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="WP_014461510.1"
                     /transl_table=11
                     /translation="MKHFTLNEILKQTGGDLCYGSGNPVIKHAINYAKKDIEDHTLIFH
                     LDREPIRGKYWKENHSIVVITDHPELCTNLEGDIILVKTAQLEEAYWKFIEYYRGLFDL
                     PVIGVTGTCGKTTTKEMIRQILIEDYKVKATWMSMNSMSVNLRYLTGIDDTTEVAVFEM
                     PVAYPGYLKVACRYFQPQIRILLNIGVHHLADCETPEEYMKAKAEIVEGLDPNSGILIL
                     NADDENIKRVLDVKHLQNVVYIGKNNDSQFQAKDIKYANGGMTFTLKHKNKEYDAFIPG
                     YGEHNVYNALAAIAAVSYVGVDISVAIQRLSLFEQVEEHLEFKRGMNECTVINDTWNSS
                     PLSMATALGVLSDMSKEKQSIALLGYMPQLGEGPYAIKQYEDMGKKAAESKIDILVVVG
                     DEAKAIGTGALKYGMDPNRVYFCKTGDEVYNVLAPYLNENTIILLKVTHRVMTKPTFKE
                     LRNKLILDNEE"
     gene            complement(13837..16458)
                     /locus_tag="IQ863_RS12900"
     CDS             complement(13837..16458)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013081818.1"
                     /locus_tag="IQ863_RS12900"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="YheC/YheD family protein"
                     /protein_id="WP_014461511.1"
                     /transl_table=11
                     /translation="MDPMREELGILSDKEMTLQTLNLNNIPSVELVDPKTCSYPVIGRK
                     YGHYSGRDIVIVNTKDQAIYEGYDYFTKIYAIDKEYCLEVEGLSVKKVQVVTSEHVVFN
                     EIPIRTQAFGWKLEQINSMDVPEMLTNVAIRALYVTGAKSGFVKMGVLENGECIVTDIN
                     SSESEWIENPLKPSLPFSMGADVEFMLSCDGELLPASTFFSVEGPVGCDERQIEQDSGE
                     YALVEVRPEKANSSTELFENIQKLIEKASAQVPYENVHFRAGSMPFSGYQCGGHIHFGI
                     PLSLSLLRALDHYLAIPVALIEESKTAKLRRKTNHGGLGRYREKPYGFEYLTLSSWIID
                     PRITLSTLALAQLVATHHHELKSEFLFHPLTQRAYYQGNKIFLKRMWKDIKANLMKTSS
                     YSYYQNELSFLFEMIEKEIPCDESNDIRRNWNAKISKEIYDRGHIIQIPKKLRLKYGLQ
                     EGQSTIISAGKAISTATVHSYPFSFRHPNMVQLSKSLRDKLSLPKDWCPKLSASEGIIT
                     LGPIIGILANRPFERQTTYFHHLCRLANEKRMLVYVFEPEDIDWEKKLVKGTTINGEGL
                     FPFPAVIYDRYFIDGRKNILIDEVRAKLQAIYKIPFVNSSNLFQLTGDKWATYELLMKE
                     YEEFLPESRLVQSPKDIAEMLDRYGEVYLKPLGGALSKGVMRIVRRPTGIFWFDLNKKE
                     LHQFSNMEELFTLLSPLMKNNPYLVQEGIRRKQHKDKNLEIRVYMQKNEKQIWLRTGMV
                     ARLTGEDVLTEDSETNMRLSKILNSLYPDPTDRRLIINQLAKISKNIVATVEEKVGPFG
                     ELAVDLCIDQYGSIKLLEINAKPDSLFSQIRAYKLRTLAGIRLLNYASSLAGYEEEKED
                     LT"
     gene            16704..17378
                     /locus_tag="IQ863_RS12905"
     CDS             16704..17378
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013081817.1"
                     /locus_tag="IQ863_RS12905"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_014461513.1"
                     /transl_table=11
                     /translation="MTLKFIHGNYLKYNKGTGFISCHVDALHAEPPAADLFTDKIVENL
                     VKKTGCAGIVSTVSRTTADLNRNLNETNYEGIQEYRRTIQEIVKSLKIVDSNNCLLTPY
                     LHLSFHGMKDTHYGPYAIEVGTLYGQSCSKEVKDWFQQALTKKAEDSFPKIHVIFDNKF
                     VGNESIAFHRQGDNKNYTGYSHNFNTFQIELSRTLRSKHRSAITNIFSEIIDDFQQTFV
                     AT"
ORIGIN
        1 ttgcttcttc aaacgtactc gctaatcccc atttattttt tatatcatga agcatatcag
       61 catattgtgc gccttgtcct ggaaataaaa atgcaacact catcgtgttt ctcctttcga
      121 ctttgtatca tccaaggaga taatggatac ttttattata aagaacatag gatcaataac
      181 tctaatacat atttcttata tatcctataa cttgaaatat ataaaaagag attgagacat
      241 aacgaaatca atgaaatcta cgttcatcct accaataaaa aatccgaacg agttagattc
      301 tccattaaga atctaactcg ttcggatttt cctttaacta aactgctttt gtcccagcct
      361 ctttttgcta catatgacct ggaaaatgcg gaagcacttc ttcaccaagc tcatgtatga
      421 cttcttcagc cggacgctta ctatcaaaaa gagcaaaaaa gaggtgatta actcctattt
      481 ctttataaat agaaagaagc tcaattaatg tattacgacc tgctcgaaat cctaaacgaa
      541 ttggcgttgg catttcattt ggatcttctg ccaaatctag atgcataggc tgtgtaaaag
      601 gtttaaaaac gcctggatga tagctgtcaa ccagtttcct ccattgtgta atagcttctg
      661 cctgaaaatg cggatgacga ggataataca tccagccgtc tccatactgt gcaaaccact
      721 ccatattttg ctgactgtat cccgtaatca tcgttggaat acgtttcgtt ggcttaggaa
      781 caacgtttgc tccttgaatg gatcccaaag cagactggat agatggaaat tctttattta
      841 acgcttcttc taaataatga aaagcctcta caaatcgctt tcctctttct tcatgaggaa
      901 tacctaaccc tcgaaaatct gcttgacggt ctccagaaga tacgcccata atcaaacgct
      961 caggaaaaag ctgatcaatg gttgccattt ctttagctat acgaagcgga tggcgtaaag
     1021 gcagcacaat agcagatgta ctaagcgcaa tttcttttgt ttgactagca agataagtca
     1081 aataaataag catatcataa atttgacccg tggctggatc accaaatgat ggatcttgaa
     1141 gaaggacatc acggaaccat aagcttgtaa acccatattg ttctgcaagc tgcgtgagct
     1201 ctacttgttt ctccatcgta ggcgtcgcat actgataatt ttcaagcggc atatgcaaac
     1261 ctaatgttaa ctcattttcc ttgtacatac gacgatatgc cttgtgatct tgaaatgtag
     1321 tcatttgctt cctctccttt tataactaag agatcatcgc aaaaagcgat gatctctttt
     1381 tttattttgc ttttgcttct attgaacggc tccatgccgt taataagacc gctcctaaaa
     1441 ccataactcc tccaatccag ggagtatgaa caagaccaat ggaatcaacg attaggccac
     1501 ctacaaaaga accaatggca atcccaacgt taaatgcagc aatattaagc gcagaggcta
     1561 catttactgc tgaagggaca tacttttcag ctaagttcac gaccagcact tgcagtccag
     1621 gtacgttcat aaatgccaat aagcccatgg caaaaatcgt gattgttccc gctactttaa
     1681 acgacgcggt aaacgataaa ataactaaga taactgcttg aattaaaaac atccaaaaca
     1741 atgcttttaa tggattttta ttagcagctt ttcctccgat cgtatttccg atagctaccg
     1801 ctacaccata taccagtagc aagatactaa ctgcgcttgg tgcgtatcct gttagctttt
     1861 caagaatagg cgctaaatac gtataagcta caaacgttcc cccataccct aaagcagtaa
     1921 tagcaaacga tagtaaaagc ggcccgtttt ttaaaatttt caattgatca ctaaactttg
     1981 ccggcggcgc ttcttttaag tttttaggta ctaaaagagc acttgcaata atagcaataa
     2041 cgccaagtaa agctacaccc caaaatgttg ctcgccaacc gaaagcttgt ccaataaatg
     2101 taccaagcgg aacacctgta acagttgcaa ctgtcaaacc tgtaaacatt aaagcaatgg
     2161 cacttgcccg cttatcttcc ggcaccaaat cagcagcgat agtcgcaccg atagaaaaga
     2221 aaatcccgtg tgaaaatgca gtaataattc gtgctataag cagcagacca aagcttgttg
     2281 ataaagctgc tactgagttt cctacgataa aaataaccat caatagcatc aacagtgatt
     2341 ttctgctcat tttacttgtt aaagctgtta atacgggagc tccaaatgca acccccattg
     2401 cgtatcctga aattaataac cctgctagtg tgatggaaat atttaaatca tctgcaagcg
     2461 atgataataa acctactgga acaaactctg tagtcccgat tccaaaggcg ctaatagcta
     2521 gcgctaatag cggcaaagca ccgctttttt tactttgttg cgtttgtgaa agtgtgttca
     2581 cttcttaacg cctcctgttc attataataa cttctttata atagcttctt ttacggctga
     2641 ttagcttagg tacctttcat atattcgcta actcgccatc aacaattgtt attatgagcg
     2701 gtgtaaaacc aaagaaaaag tacgcacttt caagtgatat aggtacctgg aggttacaat
     2761 aagcgtaaat actaaagtca tgaactattg ggtattctaa aaaaatgctt acacctagaa
     2821 aattctgcgc tatagtggta tgataaacat cagaaaaaat gcgaaaccat gcatttaacc
     2881 gttatctcta gaacaaggag agttgttaac gtgaaaaaat ataatattcc agtggaagcg
     2941 gcactagaag tcattggagg caagtggaaa gtcgttattc tttgccactt aattaaaggg
     3001 acaaagcgcc ctggagaatt aaaaagaatt atgccgcgcg ttacacaaaa agtattaacg
     3061 cagcagcttc gcgaacttga agcagacggc atcattgtta gaaagatata caatgaaatg
     3121 ccgcctcgag tagaatattc tttaacagat tacggatggt ctttaaaaag cattcttgat
     3181 tcattatgct cttggggaga acatcatatt gaaaaaacgt accctgataa agaagctgta
     3241 cttatgcagc ctgtagaaga agtcactgtt tctaattaaa aaaagctgac tagatagtca
     3301 gctttttcat tcccttactt tcgttgtttt ttaaacgatg tacgtattca ggaggaaccc
     3361 ttatgacaga acaacttaaa aagaaaatta gtcttcaaga tgctatcaaa cagcagcttg
     3421 ctaataagaa aaatcaatct tcaaataaag ctaagaacgg cagcaccaat caagcaacca
     3481 aaaagctgac gagtcaacaa accaaaaaaa cgaataatca gcgaaagaga acaggtgtat
     3541 aatgaacggt caaaatagaa aagacatagc tcctggaact aaagtcgaca ttgttttaaa
     3601 agcagatcag cgttctggaa aacttacaca cggaacggtc aaagacattc tgaccaattc
     3661 aagctttcat ccacacggca tcaaagtccg tttaacagat ggacaggaca agtcggacgt
     3721 ataaaagaaa tttacccaaa ttaaaaaagc atgtattcta catgcttttt taatttacac
     3781 tttaaataaa tctacttatt atttcctact tttgttaaaa taaagaagca ctgtcgtccc
     3841 ctttctttcc cttgggaatt cacgcaagga agaaaaaggt ggtggatagt ctgagacgaa
     3901 tcctcaagtc atttttccta gatgctgaga atgttcagct cctaacggct tacgtagagc
     3961 ttttaggagc catagtcgct cttattttgc tatttttcca gtagtaaaat aagtaatcac
     4021 cgataagtat cctctaaaag gttcttcatc cataaggctc gtgtgctgat tgtctttagg
     4081 gaatcgtgtt tggcggcact tccctatagt aaaaagacag cttccttgca gaaatggaca
     4141 aggtgtaatc aactacggcc gattatgggt gcgggacctt tttctttatt agtctatgtc
     4201 ttttttaaat ttccatacac aaaaataaaa aaacctccga gttggcggtc tcgaaggttt
     4261 tttctagtga atcgttttcg acaaatcctc atccgggcta acaaggttca gatccaataa
     4321 gattatgtga tgaatttctt caccttacaa gttacacttg tcaaagctaa gaattcattc
     4381 gcttgttaag aataactgaa cgtgcgctcc agattatcat ttttcctagc ctcagtatat
     4441 catacttgtt gaaaacaggt aaaaaaagtg ttcgacaaaa cttgtcagca gctcttcacc
     4501 tatgtcatat ttttcaaaac aaaaaacatt ttacaaatat gaaggaattt catactatta
     4561 gataaaagat aaaagataga agtattctga cttggagagg atttcaatga agcaacctca
     4621 gttagaaaaa gaaattcgag ccttacaatc agatatttac caattagcta agaaaacaag
     4681 cagctattct cacggtgaga ttttaaaatt aagtcaaaaa ctagatcaaa aaattgttag
     4741 ttaccaaaag ctttttaacc ataccaaata agagccgcgt aggactttca cttgctatcc
     4801 ctcaagtacc ataaacctac aaataaaaca gacatgtttt attcgcaata ggtacatctc
     4861 tgcttcccta attaataggg aagcttttta tttagaaaat taaaaccttc tcctttcctt
     4921 tggttagttg tcatacaaat tgggtattca atcagtagac cttaaaactt atttaagaaa
     4981 cataaaggag agaatgcacg atgagtaatg ccgatactac tgaaacaaaa aaaggcgccg
     5041 atttactagt agaaagtttg gcaagtcaga atgtgcctta tatttttggc attccaggag
     5101 ccaaaattga tggcgtattt gatgttttga aagagaaagg accagaagta attgtttgcc
     5161 gccatgaaca aaatgctgcg tttatggcag ctgctatggg aagactgaca ggtacgccgg
     5221 gcgtttgcct tgtcacatca ggtccaggtg ccgcaaatct agccactggc cttgttactg
     5281 ctacttctga aggtgatcca gtggtagccc tagcaggagc cgtcccaaga gcagatcgat
     5341 taaaaaagac gcatcaatca atggataatg aaggactatt taaatctatc gccaaattca
     5401 gcgttgaggt agaagatgct gcaaacgttc cagaagtgat aacagacgcc tttcgcgaag
     5461 ctgtttcttc tccttcagga gcatcttttg tcagcttgcc gcaggatgta atggatagcg
     5521 aaacatcagc ttccactcta tccgttctaa aaactcctac gattggcgct gctaatttag
     5581 acgctgttgc ccaagctata cacactattc gcacagcaaa attacctgtt ttacttttag
     5641 gtatgagagc aagtaaatct aaagcagtgg aatcaatccg tcaacttata gaagaacatg
     5701 accttcctgt ggtggaaacg ttccaaggtg cgggagctgt ttcaagagag cttgaaactc
     5761 acttttttgg acgagtaggt ctttttcgca atcagcctgg agatgtctta ttgaagcatg
     5821 ctgacgtcgt cttaacaatc ggatatgacc caattgaata ccctccttct ctttggaact
     5881 cagaaagtca aagaagcatt attcatttag atgatgtaca agcgcaagct gaccaccatt
     5941 atcagccgga aattgagtta gtaggggata ttccaagcac aattgacata ttaacggata
     6001 aactcccaca tttaatacgt tcaaaagaaa gtgcttcttt tttaaaaaca ttaaaaagca
     6061 aacttgaaca acctgacgat cagtacgagt tttctaatga cactcacctt catcccctac
     6121 aattaattga aattttgcag aactttgttg aagatgacac taccatcaca tgtgacgttg
     6181 gttcgcatta catatggatg gctcgttatt ttcgttccta tgaacctcag catttactat
     6241 tcagcaacgg catgcagacg ctaggtgttg ctttgccttg ggctatttca gctgcactcg
     6301 ttcgccccca taaaaaagtt ctgtccatgt ctggagacgg cggtttttta ttttcagcta
     6361 tggagctcga gacagccgtt cggttaaact taccaattgt tcacattatc tggaacgatc
     6421 aaacctacga tatggttggc tttcaacagc agattaaata tggccgaaaa tctgcggtag
     6481 agttcggcag tgtagacttt gttacgcttg ctgaaagttt tggtgccaaa ggatttagag
     6541 ttaactcacc agaagaattt tcggacactc tagcaagcgc ttttcgtgaa caatgccccg
     6601 ttatcatcga tgtgcctgta gattataaag ataatgaaaa attaaataca cagctactgc
     6661 cagatcaaat taactgaaag gactgagtaa agaatgagca atgaagaaaa attaacgatt
     6721 taccaaactt ccactatgat cgctttatta gacggagtat ttgacggtgt tgtcacttac
     6781 aaggacttag ctaaaaaggg tgatttcggc atcggtacgt ttaatcaatt ggatggtgaa
     6841 atgattgcct ttgacggaga attttatcat attgctgatg ggaaagccgc ccctattagc
     6901 gaagatgcaa aaacaccatt tgccattatg acaacctttt atgaagatat ctcttaccgt
     6961 gtagaaaagg aaatgaaccg tgaacaacta gaaaccttaa tgaacaagct tattccaagc
     7021 cctaatttat tctatgcagt tcgcattgac gggaagttca aagaagtaaa aacccgcacg
     7081 gttgcaaagc aagagaagcc ctatccttct atggtagatg cagcggctaa tcagcctaca
     7141 tttacatctt ctgatgtaaa tggaacacta gcaggttttt acacgccaga atattcctca
     7201 ggcatcggtg ttcccggcta tcacctgcat ttcattgatg aatataaaaa agaaggtgga
     7261 catgttttag acgtctccgt tcaggatgtt acaattcaaa tctgcaaaaa aacgaaatta
     7321 aatctcaatt tatcggaaac aagtgaattt ttaaatgcta atttagaaga ccatgatgta
     7381 gaagaagaaa ttgcgacggc tgaataagca acgatacttt tactgtaaaa aaactctcat
     7441 cgttacgcat gacgatgaga gttttttcac tagggttcac cccgcatatc tatcacaaca
     7501 cagaaacctt ccgcctatac gtaaaaagaa tccgtgtctt ttaaaacata gaaaaaaggt
     7561 atttcagtct catttagaca tctagtcatt cgatccttaa agttatctaa tttaatgatg
     7621 ccttttgctt cttcgaacgt aaaatatcct acatcctgcg tttcactaga aactgaaatt
     7681 tccccgctaa tcgcttttcc ctttaataaa ataacagtag tagatcgttt aacatcttgt
     7741 tctactccga gaaagtttgt cacttcaata acaattccag attcttcttt aacttctcgt
     7801 atacccgcat ctgttatcga ttcatctgct tcgacaaaac caccgggaaa ttcccatccc
     7861 cgtctatggt ttttgactag aagcacgtga tgatcttgat ttaccaccat aactgctaca
     7921 gctaattttt gattcacttt tttcactctc cttttaaagt gttaagatat tgttcaagaa
     7981 ggtgaagaaa tctttctata tctctatacg tgatattacc aatattggct attcgaaacg
     8041 tttctaattt atcgatttta ccgggataaa tagtaaatcc ttgtttataa aaaaaatcat
     8101 gcatgatatc aaaattataa ttttgatagg aaggttcgag gatagaggta ataattttag
     8161 aatgatgtgt ttcaggaacc aaataggtca acccaagccg agtaattcca tccgttaaag
     8221 tagtccatga ttttgaatag cgttcatatc gtctctcgat tccttcccac tgagtttcta
     8281 taattgcctg ctttaaggca tacatagtct gaacaggagg agtaaaacgc atttgtttat
     8341 tcatttggaa atagcgatac tgatcatata aatgaagata aaggttgcga ggcttcattt
     8401 tcttcgtttt ttctaacctt tctttatttg ctataacaaa cgacacgcct gccattcctt
     8461 gaaggttttt atttgcactt gcggccagat agcttatatt cattttttgc atgtcaattg
     8521 gaacagctgc ataggaactc attgcatcta caatcatctc gatattttcc actgcgcaaa
     8581 gtttcccaat ttgctcaata tcattaagaa gccctgttga cgtttcacaa tgaacaagcg
     8641 ccagataaga aacgttcgcc gaaatctttt ttataaaaac ttctaagtca ttcatatcaa
     8701 tagcccgctc agcggagctt ttatattctg cataatccag cccataaatt tcagcgattt
     8761 tacacattct ttcaccgtat ggaccattat taataataac aaccgtaccg ttatcaatta
     8821 ctgaacttaa aatcgattcc acggccgccg ttccagatcc gccgaatagt accgtagtat
     8881 atttatctga attcccaaca aactttgtta attcagcaga gataaactcc atcgtctctc
     8941 caaattcttt ttctctcggg caaatgtctt ccactatttg agcatatttt acggtgtctg
     9001 ttgtagttgc cggtccagga ttcaggagga tatttctttt aacggcgtgc ggaatacctt
     9061 cacgagcttt aataatcgga taaacaatag tagttgctct tgcccagtga tcttcatcat
     9121 ccacttcaca ccatgccaaa ttatccagtt tatgaacata cacatttgct ttttcggaaa
     9181 ttttaacaag cccgtattca taatcaatcg cttgatttag ctgaaacagc gtatttgctt
     9241 catcacaaag tctctgaaat gtagcataag agagtttggt taaaccaact agttctgcat
     9301 aaatactatt caactcttct ttttgttttg acatattctt aaggttatta ttttcatcag
     9361 cttcgatata cacttcatcg ccagcatcgt tcaatttact cgctagtata acatctgatc
     9421 tcgaatgatt tattaatgtt ttcaacgcac tcttttcata aattacatct gattccaata
     9481 gtaaaaaatc ttctttaata taacttttaa attgatacag cgtaaacaga cttcccgttg
     9541 tttcgtatct agaattataa acacatttta tttgagggta tctaaccatt agcttttcat
     9601 attcttcttt catataacct gttccaataa aaatcgtctt aactcctgct tctattaact
     9661 ttgaaatgga atgttcaata atcgattttt catcaatcat caaaaaacct ttagggcggt
     9721 ttccagctct tttacgaatc ctgctcccca tccccgcagc tagaataaca gctgtcttaa
     9781 tcatctaaaa acacctgcag tctttctctg acttcataag gcttcatttc aggacgcccc
     9841 aggttctttt tagatccctt cgctatttta agatataaaa atgttaattt tttatcattt
     9901 ttccactgtt tgatatacat ctctaactca tttaagttat gaacatgaat ggaattttga
     9961 tacccacagg aagaagcaat atctacaaaa ttaatgttat gagagaccgt atgttgtccc
    10021 cctgtagaat catgggtttg attgtctaaa agaatgtgta ataaattttc cggacaatag
    10081 tatccgttgg ttgctagatt tcccatgcgc ataagcaaag ctccgtctcc atcaatcgta
    10141 atgatgttct tttcatttct gctcaaagct aagcccaaac caatggaact aacgcacccc
    10201 atagatccaa ccatataaaa attatttttt gcgtcttcta tttcgtaaag ctctcttccc
    10261 gtttttcctg tagcggccaa ttgaatggta tcattgtcct ttattgaatt aactacttgt
    10321 agtgcctcat accttgaagg taactcgtcg gttgttatct gttcactttt cattttagga
    10381 gtaacgtttt tcattgattt ttctgaaagc ttaactgact caaacgttcc ttttttcaca
    10441 ataaagaagt agctttcatt attttcaata gcttgattag cccgccgtaa ttggttttta
    10501 acttcggtaa aatcaaaaga taaatattcc cattttattt tcattgtatt tagcagagca
    10561 ggagtaattt ctcccattaa ttcatgctgt ggttcatccg gaaatccagg ttctccgcga
    10621 aaactgacaa atccaaggac aggaatttta aacggatgaa taagagaggt taaaggggaa
    10681 acagcatttg ctaatccgga gttctgcatt aaaacaaccg ctttcttgcc agcaattgat
    10741 gcgccagcag caattgctac tgcttctcct tcatttgctg ctgcaacgta ttcacattcg
    10801 ttgatagcgt aattaattag aggtttaaga taggaacaag gaaccccact ataaaattga
    10861 aaacctagtt tttttaattg attcccaaat aatgatgtac taatcatttt tcccaccatc
    10921 tttttgaagc tttatatacg tttcggtatc tatccatgat cccatcgtat atttaacagc
    10981 aaccggatga attaaattaa tataattaaa aaaatcagag aacgtgagct gagcaaaatc
    11041 ttctctttgt gccagttctt ccaaaacttt ttttacaacc tcagtgcctt ttccggaaac
    11101 tttccaaaga ccaatgaatt caccatgaat gtttttagaa tttggactgg atgacatttg
    11161 aattaagtct actgttttgg aataaagctt tttggaatag ggaaattcag ccgttacata
    11221 agatgtttga tcccctttta aatcataatc agctcctgct acgatagtga tatcaccact
    11281 ttcatgaatt aactcctcaa aaacatgagt tttatacacg atgttactat cactgattaa
    11341 agtagccgtt cctagcttat ctcttgcagc ataaagatca tataattctc cttgattagg
    11401 agactccttg tcttccatag ctttgttact gtctactttt atactatttg tcacgccaac
    11461 tttttttaat tgattcattt gctcagttat taaaacgtca tttgctgatt tttttaccag
    11521 aatcatagcg ttaatactat gattggcaga gcttaaataa agtttctctg ccatatcgag
    11581 ctcttcagct ttttgaagac ggaaaacttc ctcaagcgga gagacatttt tttctacgtt
    11641 aatcaaactt ttatcgtaat aaatctgagc ggacgttttc tgcatagctg taatagaaga
    11701 tcttaaatta tggttggccc aaatgcttaa gctaattcct aaatcactaa atttatcagt
    11761 tggaaccgaa taatatttgg ttggaacaat aataaccgga agcctgtttc cccactcttt
    11821 cataaaagct tcaatctctg tcatatctga ccttttacta tgaatcaaaa cggcatcggt
    11881 tcccgcttgg cgataagctt ctgcacgttt taaagcttct ttcagccccc accctgcaat
    11941 aaatgcttct acacgcgata ccactacaaa atcttcgtca ttttggctat ctttcattgc
    12001 tttaattttg ccgcaaaatt cattaatatc tgcgagcggc tgtgcttcac ctttaataaa
    12061 tgagtttgtt tttggaaaca atttatcttc aatacatacg cctggaatgt ctcgctgctc
    12121 caacttctta actaatcttc ttgcattatt aaaattccca tagcctgtat caccatccag
    12181 aagaatggga acggacgttg catcactcat aaactctaag atatctaata cctgtgtcca
    12241 cgaagcctca ttattatctc ttacacccag tgctgcggat aaagacaggc cgctggccca
    12301 tatccctttg aaccctgctt cttctacaat ctttgcagat agcgcactgt gagcctccat
    12361 aataaactct agctgcttgc tttctatcaa ctctcttagc tgtttggtct tcttcatgtc
    12421 tatcctcctt ttactcttcg ttgtctagaa taagtttatt tcttaattct ttaaaagtag
    12481 gttttgtcat aacacgatgt gttactttta ataaaattat tgtattttca tttaaatatg
    12541 gagctagcac attgtatact tcatcaccag ttttacaaaa atagactcta ttagggtcca
    12601 ttccatattt taaagctcct gttccaatag cttttgcttc atctcccact acaactagaa
    12661 tgtcaatttt ggattccgct gctttttttc ccatatcttc atattgttta attgcataag
    12721 gtccttctcc tagctgaggc atgtagccta ataaagcgat agattgtttc tcttttgaca
    12781 tatcactgag aactccaaga gctgtagcca tcgataaagg tgaagagttc catgtgtcgt
    12841 taattaccgt acattcattc attcctcttt taaattccaa atgttcttct acttgttcaa
    12901 ataagcttag tctttgtatg gcaacggaaa tatcaacgcc cacataagat actgcagcaa
    12961 tggcagccag tgcattatac acattatgtt caccatatcc agggataaaa gcatcgtatt
    13021 ctttgttttt gtgctttaaa gtaaaagtca ttcccccatt agcatattta atatcttttg
    13081 cttgaaattg ggaatcatta ttcttaccta tatatacaac attttgaaga tgctttacat
    13141 caagcactct cttaatattt tcgtcatctg cgtttaaaat taaaatgccg ctatttggat
    13201 ctaagccttc tacaatttcg gcttttgctt tcatatattc ttcaggtgtt tcacaatcag
    13261 caagatggtg cacgcctata tttaatagaa ttcgtatttg tggctgaaag tagcggcacg
    13321 caacttttaa ataacctgga taagcgaccg gcatttcaaa aacagctact tctgtcgtat
    13381 catcaatacc tgttaaataa cgtagattta cagacataga attcatactc atccacgtag
    13441 ctttcacttt atagtcttca attaatattt gtcggatcat ctctttcgtg gtcgtttttc
    13501 cacaagttcc tgtaactcca ataacaggaa gatcaaaaag ccctcggtag tattcgataa
    13561 atttccaata agcctcttct aattgagctg ttttaacaag aataatatct ccctctaagt
    13621 ttgtgcacag ctcagggtga tctgttataa cgacaattga atgattttct ttccaatact
    13681 ttcctctaat cggttctcta tctaaatgaa agatgagtgt atgatcctct atatcctttt
    13741 tagcgtagtt tatagcatgt ttaataacag gatttcctga tccataacaa aggtctcccc
    13801 ccgtttgttt aaggatttca tttaatgtaa agtgcttcat gttaaatcct ccttttcttc
    13861 ttcgtaacca gccagtgaag acgcataatt aagcagacga atccccgcca aagtacgcag
    13921 cttataagct ctaatttgtg agaacagact atctggtttt gcatttattt caagcaactt
    13981 tatggaaccg tactgatcta tacataaatc taccgctagc tctccaaaag gacccacttt
    14041 ctcttctact gttgccacaa tatttttaga tattttagct aattggttta taatgagcct
    14101 cctgtctgtt ggatcgggat ataaactatt caatatttta ctgagacgca tattcgtttc
    14161 tgaatcttcc gttagcacat cttcaccggt gagacgcgca accatgcccg ttcgaagcca
    14221 aatttgcttt tcattttttt gcatataaac ccgtatttct aaatttttat ctttatgctg
    14281 ttttctgcga attccttctt gaacaagata aggattgttt ttcatcaaag gggagagcaa
    14341 agtaaataat tcttccatat tactgaattg atgtagctct tttttattaa gatcaaacca
    14401 aaagatgcct gttggtcggc gaacgatgcg catgactcct ttacttaagg ccccgccaag
    14461 cggttttaaa taaacttctc cgtacctatc aagcatttct gcaatgtctt taggactttg
    14521 tacgagacgt gactctggaa gaaattcttc atattctttc ataagaagtt catatgttgc
    14581 ccacttatct cccgtaagct gaaacaagtt tgaagaatta acaaaaggaa ttttataaat
    14641 cgcctgaagt tttgctctta cttcatctat taaaatattt tttctgccat ctatgaaata
    14701 acggtcataa ataacagcag gaaaaggaaa gagcccttca ccgttaatgg ttgttccttt
    14761 aaccaatttt ttctcccaat caatatcttc gggttcaaac acatatacaa gcattcgctt
    14821 ttcatttgct aagcgacata gatggtggaa ataagttgtt tgtctttcaa aaggacggtt
    14881 agcaagaatg ccaatgatag gacctaaagt tataatgcct tctgaagctg atagcttggg
    14941 gcaccaatct tttgggagag atagtttatc acggagagat ttagagagtt gaaccatatt
    15001 tggatggcga aatgaaaaag gataagaatg cacagttgcc gttgatattg cctttcctgc
    15061 actaataatc gtagactgcc cctcttggag gccgtatttt aaacggagct ttttggggat
    15121 ttggataata tgtccgcggt catagatttc tttagaaatt tttgcattcc aattcctgcg
    15181 aatatcattt gattcatcac aaggaatttc tttttcgatc atttcaaata gaaaagatag
    15241 ttcattttga tagtatgaat agctggatgt tttcataaga ttggccttga tatctttcca
    15301 cattcgttta agaaagattt tattcccttg atagtaggca cgctgcgtaa gtggatgaaa
    15361 taaaaattcg ctttttaatt catgatgatg ggtagcaact agctgagcaa gagctaaagt
    15421 ggagagagta attctagggt cgataatcca agagcttaaa gttaaatact caaatccata
    15481 cggtttctca cgatagcgtc caagacctcc atggttcgtt tttcgtctga gtttagcggt
    15541 cttagattct tctatcagcg ctacgggaat agcaaggtag tggtcaagag ctcttaataa
    15601 ggataaagaa agcggaatgc caaaatgaat atgtcctcca cattgatacc ctgaaaacgg
    15661 catgctgcct gctcgaaaat gaacattttc atatggtacc tgtgcagatg ctttttctat
    15721 taatttttgg atattctcaa atagctctgt tgatgaattc gctttttcag gcctaacttc
    15781 tactaatgca tactcaccgc tatcttgctc tatttgtctt tcatcacagc ctaccggacc
    15841 ttcaacagaa aaaaaagtag aagctggtag aagctctcca tcacaactta acataaactc
    15901 aacatcagca cccatgctaa aaggaagact aggctttaat ggattttcta tccattcact
    15961 ctctgatgag ttaatatcgg tgacgataca ctctccattt tctaataccc ccattttgac
    16021 aaagccgctt ttagctcccg ttacgtagag cgctcgaatg gctacgttcg tcagcatttc
    16081 cggtacatcc atgctgttta tctgctctaa tttccaaccg aaggcttgcg ttcgaatggg
    16141 tatttcatta aatacaacat gttcactggt tactacttgt acttttttca cgctcagtcc
    16201 ttctacttct agacaatatt ctttgtctat tgcatatatt ttcgtaaaat aatcatatcc
    16261 ttcatatatt gcttggtcct ttgtattgac gataacaata tctcttccgc tatagtgacc
    16321 atatttccta cctatcactg gatatgaaca ggtttttggg tcaactaatt caacagacgg
    16381 tatattattc agattaagag tttgtaacgt catttcttta tcagataata tccctaactc
    16441 ttctctcatt ggatccattt taaagcctcc tagttagaac tttctatcgt attttgctcc
    16501 tttaatgccc tataacaaca atcagctact catatagtat gtaactttac aaaatactcc
    16561 tgtacatata tctatttatt tccaccacct ccaaaaactt tttatgtatg tatttaactt
    16621 ctgtaataat tcaccgattt tatttttact gcatttatta tagaaaggat agatttcaaa
    16681 cgatataaaa ggagaaatag taaatgacat taaaattcat tcatggaaac tacttaaaat
    16741 acaataaagg aactggcttt ataagctgtc atgtggatgc acttcatgca gaaccacccg
    16801 ctgctgattt atttacggat aaaattgtag agaatttagt caagaaaaca ggatgtgcag
    16861 gaatcgttag tacggtttct agaaccacag cagatttaaa tagaaatcta aatgaaacaa
    16921 actatgaggg aatacaagaa tatagaagaa caatccaaga aattgttaaa tctttaaaga
    16981 tagtagatag taataattgc cttctgacac cctacctgca tttatctttt catggaatga
    17041 aggatacaca ttacgggcct tatgcaattg aagtggggac tttatatgga caatcctgct
    17101 caaaggaagt taaagattgg tttcaacagg ccctgactaa aaaagctgaa gattcgttcc
    17161 ctaaaatcca tgtaatattt gataataaat ttgttggaaa cgaatcaatt gcctttcaca
    17221 gacaaggtga caataagaac tatacaggtt acagtcataa ttttaatacg tttcaaattg
    17281 aattatcccg aactctgcgc agcaagcata gatctgctat aacgaatata ttttccgaga
    17341 ttattgatga ctttcaacaa acattcgtcg ctacttagtt tttaagaatc ttttccgtct
    17401 actatttctt ttatata
//
