LOCUS       NZ_CP064270            28751 bp    DNA     circular CON 17-APR-2024
DEFINITION  Priestia megaterium strain N447-3 chromosome, complete genome.
ACCESSION   NZ_CP064270
VERSION     NZ_CP064270.1
KEYWORDS    RefSeq.
SOURCE      Priestia megaterium (Bacillus megaterium)
  ORGANISM  Priestia megaterium
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Priestia.
REFERENCE   1  (bases 1 to 5080837)
  AUTHORS   Wang,C., Jia,Z., Zhao,J., Wang,X., Liu,Z. and Ji,F.
  TITLE     Comparative Genome Analysis of key enzyme genes in ammonia nitrogen
            Metabolism Pathway of bacteria from different sources
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5080837)
  AUTHORS   Wang,C., Jia,Z., Zhao,J., Wang,X., Liu,Z. and Ji,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-OCT-2020) Guangdong Key Laboratory of Animal
            Conservation and Resource Utilization, Guangdong Public Laboratory
            of Wild Animal Conservation and Utilization, Institute of Zoology,
            Guangdong Academy of Science, Microbial Pathogenic Ecology Group,
            105 Xingang West Road, Guangzhou 510260, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. V1.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 331.67x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022811865.1-RS_2024_04_17
            Annotation Date                   :: 04/17/2024 10:21:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,644
            CDSs (total)                      :: 5,465
            Genes (coding)                    :: 5,410
            CDSs (with protein)               :: 5,410
            Genes (RNA)                       :: 179
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 131
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 17 of 55
            Pseudo Genes (incomplete)         :: 32 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 5 of 55
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 08:04:25
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1106165
            Orig. end                         :: 1134916
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP064270.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..28751
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="lassopeptide"
                     /region_number="1"
                     /rules="(PF13471 and Asn_synthase or micJ25 or mcjC)"
                     /tool="antismash"
     cand_cluster    1..28751
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /kind="single"
                     /product="lassopeptide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..28751
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:18751](-)"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..18751)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
     gene            complement(525..1994)
                     /locus_tag="IQ863_RS05985"
     CDS             complement(525..1994)
                     /EC_number="6.3.4.21"
                     /GO_function="GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014460243.1"
                     /locus_tag="IQ863_RS05985"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="WP_014460243.1"
                     /transl_table=11
                     /translation="MNMFTDDSVMLHTDLYQINMGETYWEDNIHQKKAVFEVFFRKLPF
                     GNGYAVFAGLERIIEYIKAFKFSESDLTYLHEELGFKEDYLAYLKDLTFTGTIRCMREG
                     ELVFHNEPILRVEAPLIEAQLVETAILNIVNYQTLIATKAARIKHVVGDQVAMEFGTRR
                     AQEMDAAIWGTRAAYIGGFGATSNVRAGKKFGIPVAGTHAHAMVQAYKDEYIAFHKYAR
                     RHKNCVFLVDTYDTLKSGVPNAIKVAKELGDKINFTAIRLDSGDLTYLSKKARQMLDEA
                     GFTETKIVASNDLDEDTIMHLKSQGAQIDMWGIGTKLITAYDQPALGAVYKLVSIEGEN
                     GEMMDTIKISANPEKVSTPGLKRVYRIINNLNGKSEGDYITMEEEDPQSEERLKMFHPV
                     HTFISKFVTNFEAKELHHDIFVNGKLVYETEELQEIQQYVKDNLELLWDEYKRTRNPEE
                     YPVDLSEKCWENKTRQIAYVKQKVAEALNKK"
     gene            complement(2018..2566)
                     /locus_tag="IQ863_RS05990"
     CDS             complement(2018..2566)
                     /EC_number="3.-.-.-"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1027: isochorismatase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013056905.1"
                     /locus_tag="IQ863_RS05990"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="WP_243556889.1"
                     /transl_table=11
                     /translation="MKKALINIDYTNDFVAENGALTCGEPGRHIETSINDITRQFIEQD
                     EYVVFAIDFHKENDSLHPESALFPPHNIEGSAGRKLYGKLNDLYNVYQSKANVEWMNKT
                     RYSAFAGTELEIKLRERQITDVYLAGVCTDICVLHTAVDAYNEGFAIYIYEKAVASFNQ
                     KGHEWALEHFKSCLGAKII"
     gene            complement(2639..3415)
                     /locus_tag="IQ863_RS05995"
     CDS             complement(2639..3415)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013083025.1"
                     /locus_tag="IQ863_RS05995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NUDIX domain-containing protein"
                     /protein_id="WP_279400973.1"
                     /transl_table=11
                     /translation="MSNKKAVTNQTKKEYPKPYGYTSDIAVFTIVTEEKEPYKPPKMNL
                     KLMLIQRAMLNAEGEKNSEAGKWALPGGFVHPDESAFEAAKRELEEETGVNDIHLKHYG
                     VYDEPDRDDRGWVITNAHYAIVPEDSLIKRKANDDAARVELFKIDEIFSLPLAFDHEII
                     IRDAIKEIKKDLLQTTIAQKFLPSQFTYSELQAVLLTVTDDAAIKSDQAFARKIRMLPF
                     IEEVDGKTTTRTSKKPTKLYRFVEMNVMKPIYTARY"
     gene            complement(3637..4032)
                     /locus_tag="IQ863_RS06000"
     CDS             complement(3637..4032)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014460246.1"
                     /locus_tag="IQ863_RS06000"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_014460246.1"
                     /transl_table=11
                     /translation="MTKVEHYIQTLGNSADLLTKKQTSIYFEKLSNTFPFLTIMQINWR
                     KVLIKKSARHIEEIKKWLQEMNIKEHQVVLFWKRATKAVSVDLAQALTFFQQTADLTEE
                     AFIYCPSVDYVIEYFKDGKMMIGLAAR"
     misc_feature    complement(4069..4087)
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 22.77"
                     /tool="antismash"
     gene            4364..5488
                     /locus_tag="IQ863_RS06005"
     CDS             4364..5488
                     /GO_function="GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1050: monooxygenase FAD-binding"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010676616.1"
                     /locus_tag="IQ863_RS06005"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="FAD-dependent monooxygenase"
                     /protein_id="WP_048021058.1"
                     /transl_table=11
                     /translation="MNYQTDVCIIGAGPGGALLAYLLAKQNISTILIERSDKLGKEFRG
                     EHLNEDGEMILKKHHIFEEIESLGLLRMSRVEYWKDGHVFKTIESELGHAGIHVPQQHL
                     LQSINRQASLYPSYKLMLGTKVTELMQNEKGQYTGIKAEQKGRGEIIVESKIIIGADGR
                     YSTVRKLAHIKNNIHNPGYDLLWAKIPAPEKWKPTIRFTVVNNQQMALFTQTGGFIQIG
                     WNIKKGSYTSLKKQSVQPFLDQLILAFPQLKESIEQNLHSWKNFILLSVYSSHCDTWVK
                     DGLAIIGDAAHTMTPTGAYGMNAAIMDADVLSQILPKLLSHGSLEAGQLKKFENARRKI
                     VEDLQDQQAMTEASFIENFSAILKIQEERLPLFQ"
     gene            complement(5591..6517)
                     /locus_tag="IQ863_RS06010"
     CDS             complement(5591..6517)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /locus_tag="IQ863_RS06010"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="metallophosphoesterase"
                     /protein_id="WP_230683513.1"
                     /transl_table=11
                     /translation="MKGKVIAASFFLIVVLIGYTVWDNQRITVAKQDIIIKHLPKELEG
                     FSIVQITDLHEKEFGENQERLINTINSIHYDAIVFTGDMMKNNSENAVPFYTLIEGIEN
                     KQHALFVHGNTDPKSYRTSSSNLEKGKFVKDMESKGVRLLDSTYTVKRGNAHIQFVNFD
                     WSLKTENKSKTSGSHLSNNKGYPEKETSDLVIGLNHFPVVDSHIDALLRDPSVTFRYYD
                     LIMAGHYHGGQFRIPFFGALFVPEAWYEKSGLFPPQDRVKGLWEYKHIKQYVSAGLGSN
                     GPIPFLNFRFFDSPEINVLTFKSRKTE"
     gene            complement(6605..7708)
                     /locus_tag="IQ863_RS06015"
     CDS             complement(6605..7708)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) NTP_transf_5"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383226.1"
                     /locus_tag="IQ863_RS06015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="nucleotidyltransferase family protein"
                     /protein_id="WP_080968661.1"
                     /sec_met_domain="NTP_transf_5 (E-value: 2.7e-38, bitscore:
                     130.1, seeds: 77, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLDKLITHLYDSNANLPSDDEFYYEVIKEIELYFISPQVYSLLKE
                     KGELGNTPLFFQTRLKQKYQETLYQNLFIKNQTDHIIRKFEEEQIEVIPLKGVYFAEKY
                     FGHIGARGTSDIDILVKRKDINKAIEVVKSMDFKIEEEFIPSHFHVSFSREIPGSAIPL
                     TVEIHWDILKEKTANFNIQEFWGEAVRLEGFQFVNALSHYHTFYMICLHGWRHNLDSPK
                     HFLDIIQMIDVVKDKISYETLFKDAKRHQTYKRMIRTLSIVYSQYSILEQIKELSSIEN
                     QFKGFASSTLEQEKKGIKKYFTFMDYQFFSYDSVWHSLSEMRSWLLPNRFEVTSQLKET
                     SKQHSYFYSLLMLYKQRCNSVVKVLWS"
     gene            complement(7727..9538)
                     /locus_tag="IQ863_RS06020"
     CDS             complement(7727..9538)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="resistance (resist) ABC_efflux"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383225.1"
                     /locus_tag="IQ863_RS06020"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_048021060.1"
                     /transl_table=11
                     /translation="MISLKKIKQFDIREYISIKETKSMVQWLWPYVMKHKGAYIGLFIL
                     LFVEITLTLVSAWFIGNITDAAVHGNFERLKWLVAIGVGLSVTSITANFIDTYLETIAV
                     NAVKRDLNLDLYKHILLLRERDVSNLHSGELLSHFTNDIHNVEGVIGRGLINLVRFPII
                     SIAAFIYLVQINWKLSLSIVLVSPIAIAGGAIFGILLRNNSRIMNELISNIIKKLNDTF
                     HGFTVIRSFTLEKLFYRDYRQKNEQLYSLEMREAKLRGWFYAGSEAIGAIAFLISLIIG
                     AFFVTDKIITIGALLTFINLVNQLISPLTGLAGQWAGYQRSVSALERITKLLNYPTQAK
                     ALPHYTSATLVQKDIQVRNLTFSYDESAIVFKDFNLYIPAGKAVALVGPSGAGKSTLFN
                     LIQHFYEPHFGTISIDGRSIDELTISELRSSIAYVPQETFLFSGTIKDNLMIARPLATE
                     EEMINAAVHANIHDHIMTLRNGYETEVGERGAKLSGGQRQRLAIARAILKDAPILLLDE
                     ATSALDSETEHLVKEALARLMINRTTIMIAHRLSTIQHADLIVVMDQGEVVQKGTHEEL
                     IRQSGMYRRLNNAQEISQQNPIMLKAK"
     gene            complement(9535..9972)
                     /locus_tag="IQ863_RS06025"
     CDS             complement(9535..9972)
                     /EC_number="3.4.21.89"
                     /GO_function="GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR02228.1"
                     /locus_tag="IQ863_RS06025"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="signal peptidase I"
                     /protein_id="WP_243556891.1"
                     /transl_table=11
                     /translation="MIDRNGLLELPAEGTSMYPLIKQGDICQFTSIKHSPLKRGDIVLF
                     ESPANKLIAHRLLNCSYVQEEEYYIFKGDTNYGVDDLVTREKIIGKLVVIQKKSVAIQE
                     KNVIAVLWGKLILAFPLLSVLLRKYINYQEWKKQIERRMSL"
     gene            complement(10001..10270)
                     /locus_tag="IQ863_RS06030"
     CDS             complement(10001..10270)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: Stand_Alone_Lasso_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PqqD_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF05402"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /locus_tag="IQ863_RS06030"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="PqqD family protein"
                     /protein_id="WP_115653802.1"
                     /sec_met_domain="Stand_Alone_Lasso_RRE (E-value: 5.3e-16,
                     bitscore: 56.7, seeds: 25, tool: rule-based-clusters)"
                     /sec_met_domain="PqqD_RRE (E-value: 5.2e-07, bitscore:
                     27.6, seeds: 9, tool: rule-based-clusters)"
                     /sec_met_domain="PF05402 (E-value: 1.2e-15, bitscore: 55.8,
                     seeds: 461, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTSYIQKGGYEVMEVDSEWVILNSHAYTVTTLNEVGGYCWKVIEN
                     KKTLAEIIKAVAAYYNTEAEMVEGDIQHFLNKLIDCGLVEKYAS"
     aSDomain        complement(10013..10252)
                     /aSDomain="Stand_Alone_Lasso_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="Stand-alone RRE protein in a lasso peptide
                     cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_IQ863_RS06030_Stand_Alone_Lasso_RRE.1
                     "
                     /evalue="2.90E-18"
                     /identifier="RREFam002.1"
                     /label="IQ863_RS06030"
                     /locus_tag="IQ863_RS06030"
                     /protein_end="86"
                     /protein_start="6"
                     /score="56.7"
                     /tool="antismash"
                     /translation="KGGYEVMEVDSEWVILNSHAYTVTTLNEVGGYCWKVIENKKTLAE
                     IIKAVAAYYNTEAEMVEGDIQHFLNKLIDCGLVEK"
     aSDomain        complement(10016..10228)
                     /aSDomain="PqqD_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="RRE-containing protein in a pyrroloquinoline
                     cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_IQ863_RS06030_PqqD_RRE.1"
                     /evalue="2.80E-09"
                     /identifier="RREFam006.1"
                     /label="IQ863_RS06030"
                     /locus_tag="IQ863_RS06030"
                     /protein_end="85"
                     /protein_start="14"
                     /score="27.6"
                     /tool="antismash"
                     /translation="VDSEWVILNSHAYTVTTLNEVGGYCWKVIENKKTLAEIIKAVAAY
                     YNTEAEMVEGDIQHFLNKLIDCGLVE"
     gene            complement(10267..11052)
                     /locus_tag="IQ863_RS06035"
     CDS             complement(10267..11052)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383222.1"
                     /locus_tag="IQ863_RS06035"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_048021062.1"
                     /transl_table=11
                     /translation="MKTLHTKIGDHYIDIKTEFDDVWKIIKQYFLCVSIAKNQPDMTVT
                     ITNGYGVSFQDYEVAIEEKLNTITFKRADYLIEVDSEYKLANVAIHDELALKHALMNLY
                     SSFIVHHNWGILLHSSCVINSGKAHIFTGHSGAGKSTAAKLSMPRELLSDEATILRITD
                     ESVTVFNSPFRSELKAKGSEKNTPLESIQLLHQSISNQRIEVRRIEALMKLIDKVFYWS
                     YKPEETKTIMGLLQLLVKQVPVYELHFQKNNTFWELMSS"
     gene            complement(11162..11365)
                     /locus_tag="IQ863_RS06040"
     CDS             complement(11162..11365)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="IQ863_RS06040"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_048021064.1"
                     /transl_table=11
                     /translation="MQEKTYSAPEVLNHQLIRFETAQSWNPGKGNLDHPGKGNNGSNWP
                     STNPNPTPGGAGAPGKGKGKNK"
     gene            complement(11445..13238)
                     /locus_tag="IQ863_RS06045"
     CDS             complement(11445..13238)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020061976.1"
                     /locus_tag="IQ863_RS06045"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_243556893.1"
                     /transl_table=11
                     /translation="MKQLVYFLKQIHSFAGKRLYINLLVTVFMSLLEGIGILLLIPMIS
                     LSGIVDFDTGGPSILRVFSFLQNTPVSMTLLLILSIYVLIVTGQTILQRQITIQNRVIQ
                     LGFLRHMQMETYSTLLHANWEFFIKKRKSDLINIVLTEIARTSGGTTSFLQFIASLIFT
                     CIQIGLAFWLSPKITLFVLLCGVVIIFLNRKFLKRSLTLGNRNFELGKSYLAGITDQIN
                     GIKDIKSNTLEESRMNWYRSITEEMKNEQVEYTKLNTTSQSYYKISSAVLVAAFIYAAV
                     KLFHAEASELMLIILIFSRLWPKVAGIQASMEQISTTLPAFKAVKALQDECKNAREFEK
                     EANQSVEPVQFKKDIQCRNVYFRYNQGASYALQHINLIIPSNQMTAFVGRSGAGKSTLI
                     DLLMGLNQPEKGQVLIDGIPLSKDNLLSLRRAISYVPQDPFLFNASIRENLLLVEPNAS
                     EQRMWEALEFSSAAEFVRRLPQGLDSLIGDRGIKLSGGERQRLVLARAILRNPSILVLD
                     EATSALDTENEAKIQEALERLKGKMTIIVIAHRLSTIRNADQVIVLEQGKIIQQGGFTQ
                     LAEEKSNTFSQLLGKQMEAAH"
     gene            complement(13238..14431)
                     /locus_tag="IQ863_RS06050"
     CDS             complement(13238..14431)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) NTP_transf_5"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002160862.1"
                     /locus_tag="IQ863_RS06050"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="nucleotidyltransferase family protein"
                     /protein_id="WP_108674683.1"
                     /sec_met_domain="NTP_transf_5 (E-value: 8.4e-58, bitscore:
                     194.0, seeds: 77, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDNILNLDRMPKELKLILELLKEGNEQYLEKYSEGLCRDIDWNLF
                     IKLTLHHRVYPAINFKIKMLDADVVPTYVVQFLNRQYKRNTFQMLRLSAEMEKVSLLFS
                     KHQIQTLFLKGPILAKCLYGDISLRTSSDLDFLIPIQDLAKAEDLLVSLGYEKDDYIHT
                     VLNDWKWRHHHVTYIHPHTEIKLEIHWRLHPGPGKEPCFDELWKRKEKSSITSYPVYLL
                     GTEDLFQFLVAHGARHGWSRLRWLIDIQQIVKRKIDWVKLYTLFTTYHNPYVGGQAIIL
                     ASQLLNVRVEKKMKPLLISKRSQKLAQEAVFYLETMINLHTDSVPEYVSKYHSRHLFSL
                     MSAQQKLFFTASLLHPYPEDAEMLPLPKKLHFLYFPLRPVLWAWRKTKKDELRKGRT"
     gene            complement(14424..14906)
                     /locus_tag="IQ863_RS06055"
     CDS             complement(14424..14906)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: PF13471"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017378577.1"
                     /locus_tag="IQ863_RS06055"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="WP_048021070.1"
                     /sec_met_domain="PF13471 (E-value: 9.3e-24, bitscore: 81.9,
                     seeds: 84, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKILRKITVFLKLNMRAKLLLIEAFIYLGWARFLKTLPFSKISPS
                     LGQQMKETAFTTSSSDDKVLKDIAQSLHIMSRYTFWESQCLVKAIAGMKMLEKRKIEST
                     LYLGTAKDENGKLIAHAWLRSGPFFISGAEGKAKFTVIAKFAKEISVKKREGENYG"
     gene            complement(14939..15244)
                     /locus_tag="IQ863_RS06060"
     CDS             complement(14939..15244)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: Stand_Alone_Lasso_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF05402"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Thurincin_rSAM_RRE"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017378578.1"
                     /locus_tag="IQ863_RS06060"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="lasso peptide biosynthesis PqqD family chaperone"
                     /protein_id="WP_048021072.1"
                     /sec_met_domain="Stand_Alone_Lasso_RRE (E-value: 6.3e-27,
                     bitscore: 91.7, seeds: 25, tool: rule-based-clusters)"
                     /sec_met_domain="PF05402 (E-value: 3.8e-16, bitscore: 57.4,
                     seeds: 461, tool: rule-based-clusters)"
                     /sec_met_domain="Thurincin_rSAM_RRE (E-value: 9.1e-07,
                     bitscore: 27.1, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIKNQDISMNQLVSQETGHIVSDMDGEKVMLSISNGKYYNLGEIG
                     GEIWEKIKEPISVSELISTLVTQYNVEQSDCEEQVIAFLSGLIKEGLIQVAENNRS"
     aSDomain        complement(14960..15202)
                     /aSDomain="Stand_Alone_Lasso_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="Stand-alone RRE protein in a lasso peptide
                     cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_IQ863_RS06060_Stand_Alone_Lasso_RRE.1
                     "
                     /evalue="3.50E-29"
                     /identifier="RREFam002.1"
                     /label="IQ863_RS06060"
                     /locus_tag="IQ863_RS06060"
                     /protein_end="95"
                     /protein_start="14"
                     /score="91.7"
                     /tool="antismash"
                     /translation="QETGHIVSDMDGEKVMLSISNGKYYNLGEIGGEIWEKIKEPISVS
                     ELISTLVTQYNVEQSDCEEQVIAFLSGLIKEGLIQV"
     aSDomain        complement(14972..15151)
                     /aSDomain="Thurincin_rSAM_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="RRE-containing rSAM protein in a thurincin
                     sactipeptide cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_IQ863_RS06060_Thurincin_rSAM_RRE.1"
                     /evalue="4.90E-09"
                     /identifier="RREFam019.1"
                     /label="IQ863_RS06060"
                     /locus_tag="IQ863_RS06060"
                     /protein_end="91"
                     /protein_start="31"
                     /score="27.1"
                     /tool="antismash"
                     /translation="SISNGKYYNLGEIGGEIWEKIKEPISVSELISTLVTQYNVEQSDC
                     EEQVIAFLSGLIKEG"
     gene            complement(15241..16173)
                     /locus_tag="IQ863_RS06065"
     CDS             complement(15241..16173)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017378579.1"
                     /locus_tag="IQ863_RS06065"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="aldolase"
                     /protein_id="WP_048021074.1"
                     /transl_table=11
                     /translation="MIDTTKKVVYEAFGLKLLSEIPLPELPEISISGGSVEIIVKKGDL
                     TTLWSEKNKSGAYFIVKENLCMFKVPGLAIYLIQDGKEITVSPTEGFNEDLSRLYILGT
                     CMGALLIQRKIFPLHGSAIAIDGKAYAVVGESGAGKSTLASAFLKRGYRLLSDDVIPVT
                     FTEENIPIVTPAYPQQKLWQESLTQLGMESTCYRPIFDRETKFTIPVESQFATEAYPLA
                     GIFELIKNDSNEIKIQSIEKLERLHMLFNHTYRNFFIELSGLMKWHFDASAKIVNKVDL
                     YRLHRPISGFTAYDLTDLILTTIKRRENK"
     gene            complement(16641..16796)
                     /locus_tag="IQ863_RS06070"
     CDS             complement(16641..16796)
                     /codon_start=1
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002160858.1"
                     /locus_tag="IQ863_RS06070"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="paeninodin family lasso peptide"
                     /protein_id="WP_108674682.1"
                     /transl_table=11
                     /translation="MKKEWQIPVLETLDITMTMASTVYGPYTDEAYVPGKDVDKDNPGT
                     WKRFTS"
     CDS_motif       complement(16701..16796)
                     /prepeptide="leader"
                     /note="peptide class: lassopeptide"
                     /note="predicted sequence:
                     MKKEWQIPVLETLDITMTMASTVYGPYTDEAY"
                     /locus_tag="IQ863_RS06070_lassopeptide"
                     /aSTool="lassopeptides"
                     /tool="antismash"
     CDS_motif       complement(16641..16700)
                     /prepeptide="core"
                     /locus_tag="IQ863_RS06070_lassopeptide"
                     /peptide="lassopeptide"
                     /predicted_class="Class II"
                     /score="-12.10"
                     /molecular_weight="2129.3"
                     /monoisotopic_mass="2128.1"
                     /core_sequence="VPGKDVDKDNPGTWKRFTS"
                     /aSTool="lassopeptides"
                     /tool="antismash"
                     /leader_sequence="MKKEWQIPVLETLDITMTMASTVYGPYTDEAY"
                     /leader_location="[16700:16796](-)"
     gene            complement(16811..18751)
                     /locus_tag="IQ863_RS06075"
     CDS             complement(16811..18751)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: Asn_synthase"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) GATase_7"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1177: asparagine synthase (glutamine-hydrolyzing)"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017378582.1"
                     /locus_tag="IQ863_RS06075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="asparagine synthase-related protein"
                     /protein_id="WP_208732356.1"
                     /sec_met_domain="GATase_7 (E-value: 5.8e-21, bitscore:
                     72.9, seeds: 307, tool: rule-based-clusters)"
                     /sec_met_domain="Asn_synthase (E-value: 7.9e-22, bitscore:
                     76.6, seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSAIAGIYHSNNEPVPVEHVNRVMQSLQKYPANDIQISHKKNIFL
                     GCHAQWITPESVGEQQPYCDYERQLAITADVIIDNRDELFEKLQVKYIERKAMTDSQLI
                     LLAYYKWGEESPKYLVGDFAFVIWDERKQILFGARDFSGSRTLYFYHDTNRFAFCTTIE
                     PLLSLPYVEKKLNEQWLAEFLAISGMVDVVDASLTPYKNIAQMPPAHSITVMEETVKVK
                     QYCTLTSVQTLKLKSNEEYIEAFQEVFQEAVTSRLRTYRQVGAQLSGGLDSGSIVSFAA
                     KSLSGTRKQLYTFSYVPPNDFKDFTPKHLMPNETPFIQSTVQHIGGIKDHYLDFEGRDS
                     YSEIDDFLNTMEMPYKFFENSFWLKGMFEKASEENVGVLLNGGRGNLSISWGSAIDYYA
                     LLLKKLKWIRLASELHHYSHKAGGSRFRRIPVIARVAFPFIERLFPLGDSYEFPMLINP
                     EFAKRTGVFHKLQNYGIDETGWFSATNIYEQRKRHFEDIFHWNASNTLATKLSLRYPLW
                     KRDPTNDLRVIRFCLSLPEEQYVQKGMDRALIRRATEKLLPDTVRLNQRIRGVQGADWV
                     HRMMPHWSEFTDELHQLSKDQTILEFLDGKGIKEAMSNIHEGVHAKHAINPYYKIMMRS
                     LIVHRFIKKFN"
     gene            19129..23103
                     /locus_tag="IQ863_RS06080"
     CDS             19129..23103
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024686.4"
                     /locus_tag="IQ863_RS06080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="WP_243556895.1"
                     /transl_table=11
                     /translation="MAYKIQISSSLKKDLPTLGIGEFGLCTDTAELFIGSDSGNIQIAN
                     KSEIGLLSNLTTIDKENLVNAINEIERKISDNHGATSLDELSDVDISTLSPKNGDVLKY
                     EAGIWKPATNLSGTISSSPYVIELNRWNIKNDGTSSKTTTQGINDAIIWAKSQGVNHVI
                     LPSGFYKLKLKPWVWQDAASLSFTCINMVNDLHFEMADGCILELEGNSSPGYSIFQVKD
                     VKNVKISGGKIIGDRKTHYYEIGVKFVRGGINPDGSLNDNLNFIRSEIIDRFKDPGLLN
                     NFRLWKIEGIDVSKGYSFYQYKDTVSNNTLVGFRNNGQFAPSSPTGRGWLLQPNIGANN
                     KMVFTIDITNTPLTDNQIAAITAKVDNERYTHEWGQGIEIAGSNHVEIKNTEICDCTGD
                     AVSTGWLQYRTNSSDYTQNEMGHHIWVHGCDIHHCRRQGITLASANDVYIYDNKIHHIG
                     KADDNVTSDFRNGIPPMFAIDIESMVGESNIPYKQPYYNKDGLELNFRLYIFNNHIHDN
                     ERGHFVNADGNHVTVENNIFEGWNVGGVSSYPTNMYIKYLNNTFINTEFIVSGDNYVNG
                     LVGQQSNLRLMDVRGSVIQDIRLNNGIFYGNANYGYFGTPTVDVSTSSFIINNHGMGNT
                     AKICFEQWVGKVPSSISVDKIYYVVNRTTNSFQVSETEDGPPVTFYDSGEWGFNVSRFN
                     YGRCYINNVVIERDWNSGGTTPGFAVTAAGSIIQNVTVKNHSVSINGNGSNIYVGRPLT
                     VDGLTIIDGSANIKDCNVLNSQFIRNRNKSISPDLKFGDSAFPETKVNIKNSLFRNIDA
                     TLINSTVDNCSFFNVKLSKTDNNTQTILNHCYLENSIVDGYWLELKKGYLITRCTLLNT
                     AMTVNKNPGTTLLLENIDITNSTADLNAPRVTVNPNGGIYNTDKTVTLLANEDAIIYYT
                     LDGTLPTTSSIQYSTPLKLSNPTTLKVIAVDAAGNQSKVKSVQFNIDKTPPTNVTNLTA
                     APFNDKVDLSWTASTSNDVLNYEVYNGDTLIRSINSTTYTVNGLLAASQYSFTVKTVDK
                     AGNKSSGISIIVTTSQDTTPPEEISNLLASSITSDSIGLTWTRSLSSDVASYEIYQDSV
                     LLNGNVTTTNYSITGLKANIQYKLTVKSKDINGNVSDGISVTVETSAVSANYVTNGLIL
                     YKDNPSNNLIIENPDSYFMVNDTFTVSFTAKVPKFGDLISRYELGGSQNKILLERNGNN
                     AFGFSIWGSTANTTVTSPQYADESLYYHIDVIRTGTALLMYINNVKVSEAGIANNVYTK
                     PSSLPLIIGDKAVPVLFKNVAYYNRILTPEELALNFNALK"
     gene            complement(23412..24963)
                     /locus_tag="IQ863_RS06085"
                     /pseudo=""
     CDS             complement(23412..24963)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016105880.1"
                     /locus_tag="IQ863_RS06085"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MRTKHSIINIATGLGNQIIITSLSFISRTVFITYLGVEYLGISGL
                     FTSILAMLALAEAGVGSSIVYSLYKPVAENDQEKINVLMNLYKKAYMVIALIVLLVGLS
                     IMPFLELFTKDTSVENIHLIYLIFLINTVLPYLYLHKNNF"
     gene            complement(25402..26610)
                     /locus_tag="IQ863_RS06090"
     CDS             complement(25402..26610)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002191261.1"
                     /locus_tag="IQ863_RS06090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="WP_243549234.1"
                     /transl_table=11
                     /translation="MQDRKGHRNDYSNKCFLLITLIVIVILIDIIPIFKDWLGRIHIGR
                     YDNKNIWNKLITEKAVSWLNDTPKIKVTDNTRLIVLDMLKGNYTKSAIQHWQEASILLG
                     LSEYLKSIEDHKAENEIVKFLNKKFDSAGQWIKKPVHVDGAILAYAVMKLDFIETDTYR
                     KALDYTWEMIKEHIGEEGTVEYRKSMPSYRYVDTIGFICPFLIAYGTKYNKGECIELAV
                     KQIKEYERYGMLEIYSIPCHAYKVDNKIPLGLYGWGRGLGWYAIGLIDSWAELPTDSRH
                     KSILEESVKKFAKAAMSFQQDNGSWNWTVTRNESRADSSTTATLGWFLINASKIDGISK
                     ECLDSTTKAINYLMSVTRRTGEVDFSQGDTKDIGVYAMLFNILPFTQGFCIRMINAISI
                     TKK"
     gene            complement(26600..27762)
                     /locus_tag="IQ863_RS06095"
                     /pseudo=""
     CDS             complement(26600..27762)
                     /GO_function="GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016131138.1"
                     /locus_tag="IQ863_RS06095"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="glycosyltransferase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MNNLSCGGAEKALISLLETIDYSKFNVDLFLFKHEGLFFRKYLNK
                     "
     gene            complement(27799..28509)
                     /locus_tag="IQ863_RS28275"
     CDS             complement(27799..28509)
                     /EC_number="2.4.-.-"
                     /GO_function="GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1045: glycosyl transferase group 1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /locus_tag="IQ863_RS28275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="glycosyltransferase"
                     /protein_id="WP_341715527.1"
                     /sec_met_domain="Glycos_transf_1 (E-value: 3.5e-33,
                     bitscore: 112.9, seeds: 63, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDPKFDIRYFRKLNNIITVSEECGNILKSRFPSERNKVRIMYNIV
                     SPKLINKMAKQVKMGVYDKKCNEIVILSIGRLHYQKGFELAIKACRKLVDKGFNIKWNI
                     IGDGEERENLTKLIKINNLENHVILLGLKSNPYPYIKQADIYAQTSRFEGKSIAIDEAK
                     ILQKPIIVTNFSTARDQITDGVNGLIVDMNSIAISEGIEQLIKDFELKKTIISNLSKEE
                     LGTEEEINKLYEIL"
ORIGIN
        1 gaatgactaa tagctttcaa catgtctatt ccctctcttg ccatgatgat aaactggttt
       61 tcacttataa gttcatctgc tttcttccac ttgccctcac caatatcttg cagtaaatcg
      121 gctcccataa taaaaaataa gtcagcgtgt gggagcaatt ttttaaagtg ctccatagtg
      181 tagtatgtgt aatgatagcc gggcaatacg tctagctcat acgtatcgag caaaaatttc
      241 gggttgtctt taatcgctaa gctaaccata tttacacgat gttcatcgga cgtttggagc
      301 tttttatctg tccgtttgtg cgatgaaggc aagaagataa tagcatctaa cttctttcga
      361 tgcgcaaccg ttgatgccgt ccaaagatga acgttggtaa ccggatcaaa agaagaacca
      421 taaatcccaa ttttcgctcc tttttctaaa agcttatcta ttttcttatc agggtttagc
      481 tgttgaaaat aagcaagcgt atctgacatg tctccagctc cctactattt tttatttagg
      541 gcttccgcta ctttttgttt tacgtacgca atttgacgcg ttttattttc ccaacacttt
      601 tcgcttaaat ctactggata ttcttctgga ttgcgcgtgc gcttatattc atcccaaagc
      661 agctctaaat tatcttttac gtactgctga atttcctgaa gttcttctgt ttcatacaca
      721 agctttccgt ttacaaagat atcatgatgg agttcttttg cctcaaaatt tgtgacaaat
      781 ttactgataa acgtatgaac aggatggaac atttttaaac gttcttcgct ttgaggatct
      841 tcttcttcca tcgtgatata atccccttca gattttccat tcaaattatt gataatacga
      901 tatacgcgct ttaaccctgg tgtactcact ttttcaggat tcgcgctaat tttaatcgta
      961 tccatcatct cgccattttc tccttcaata gaaacgagtt tatacactgc tccaagcgcc
     1021 ggctgatcat atgcagtaat gagcttcgta ccaattcccc acatgtctat ctgtgcacct
     1081 tgcgacttta aatgcataat cgtatcttca tctaaatcgt tggaagcaac aatttttgtt
     1141 tctgtaaacc ctgcttcatc caacatttga cgagcttttt tagacaaata agttaaatcg
     1201 ccgctgtcta agcgaatcgc cgtaaaatta attttatcac ctaattcttt tgctacttta
     1261 atcgcattgg gaactcctga ttttaacgta tcgtacgtat caactagaaa cacgcagttt
     1321 ttgtgacgac gagcgtattt atgaaatgca atatattcgt ccttataagc ttgcaccatg
     1381 gcatgggcat gcgttcctgc aactggaatt ccaaatttct ttcctgctcg cacgttagaa
     1441 gtagcaccaa atccgccaat ataggctgct ctcgttcccc aaatagcagc atccatttct
     1501 tgagcgcgtc gtgttccaaa ctccattgct acctgatctc ctactacatg tttgattcga
     1561 gctgctttcg tcgcaatcag cgtttggtaa ttcacaatat ttaaaatcgc tgtttctacg
     1621 agctgagctt caattaaggg tgcctccaca cgtaaaattg gttcattatg aaacacaagc
     1681 tcaccttcac gcatacatcg aattgttccc gtaaatgtaa ggtcttttaa gtaagctaaa
     1741 taatcctctt taaaacctaa ttcttcgtgc aagtaggtta aatctgattc gctaaattta
     1801 aatgctttaa tatattcaat aattctttct aatccagcaa aaacagcata cccattccca
     1861 aaaggaagtt tgcggaagaa aacttcaaaa acagcctttt tttgatgaat gttatcttcc
     1921 caatacgttt ctcccatatt aatttgatat aaatcggtgt gtagcattac gctgtcatct
     1981 gtaaacatat tcattgattt cctccacttt tatgtcctta aataattttt gctccgaggc
     2041 aagatttaaa gtgttcaagc gcccactcat ggcctttttg attaaagctc gctacagctt
     2101 tttcataaat ataaatagca aatccttcat tataggcatc tactgctgta tgaagtacac
     2161 agatatccgt acatacacct gctaagtaga catcggtaat ttgtctttct cgcagtttaa
     2221 tctccagctc cgttccagca aatgcgctgt atctcgtttt attcatccat tctacatttg
     2281 cctttgattg atacacgtta tataaatcat ttaactttcc gtatagcttt cttcctgcag
     2341 aaccttcaat attatgaggc ggaaacagcg ctgattctgg atgcaaggaa tcattttctt
     2401 tatgaaaatc aatggcaaac acgacatact cgtcttgctc aataaattga cgcgtaatat
     2461 cgtttatgga tgtttcaatg tgtcgccctg gttctccgca tgtcaaagct ccgttttcag
     2521 ctacaaaatc atttgtataa tcgatgttaa tcagtgcttt tttcattatg tttccccctc
     2581 ataagtaaaa attaacagtt aacatattgt taattatttg aattgaaagt tttttctttt
     2641 aatagcgtgc tgtatagatt ggcttcatca cattcatctc gacaaaccgg tagagctttg
     2701 ttggcttttt agacgttctc gtcgttgttt ttccgtctac ttcttcaata aatggcagca
     2761 ttcttatttt tcgtgcaaac gcctgatcac ttttaattgc cgcatcatcc gttacggtaa
     2821 gcagaacagc ctgcagttca gagtatgtaa actgagaagg cagaaacttc tgagcaatgg
     2881 tcgtttgcaa taaatctttc ttaatttcct tgattgcatc tcgaatgatg atttcatgat
     2941 caaaagctaa aggcaatgaa aagatttcat ctattttaaa taattcaact cgagcagcat
     3001 catcattcgc ttttcgtttt attagactat cttctggaac aattgcataa tgtgcgtttg
     3061 tgatgaccca tcccctatca tctctgtcgg gctcatcata gacaccatag tgttttaaat
     3121 gaatatcgtt cacgcccgtt tcttcttcga gctctctttt agccgcttca aaagctgatt
     3181 catcaggatg gacaaatccg ccgggaagcg cccactttcc tgcttcgctg ttcttttctc
     3241 cctctgcgtt tagcattgct cgctggataa gcattaattt caaattcatt ttaggtggtt
     3301 tgtatggttc tttttcttcc gtcacaatcg taaatacagc aatatctgac gtataaccat
     3361 agggtttagg gtattctttt ttcgtttgat tcgtcactgc ttttttgttc gacattcagc
     3421 atttctcctg atcactcttt ttacaattaa caatatgatt aatgttaatt atatttgttt
     3481 tatggaaaat gtcaatactt ttcccactaa ttataaagag aaggtaattt atgttgtaaa
     3541 taacgttatg tttcgtattt tttagagttt atatgcataa aaagccgagt gaagaagttc
     3601 ccattcgcct cttccctcag cttttttccc ttttaattag cgtgctgcta aaccaatcat
     3661 cattttaccg tctttaaaat attcaatgac atagtcaaca gacggacagt aaataaacgc
     3721 ttcttctgta agatcagctg tttgttgaaa aaacgttaaa gcctgagcta aatcgacgct
     3781 tacggcttta gttgctcgtt tccaaaataa aacaacctga tgttctttta tattcatctc
     3841 ttgaagccat tttttgattt cttcaatatg ccgggccgac tttttaatta atacttttct
     3901 ccaattaatt tgcatgattg ttaaaaaagg aaaggtgttt gaaagttttt cgaagtaaat
     3961 gcttgtctgc tttttcgtta gcaaatctgc actgttaccc aatgtttgaa tataatgttc
     4021 tacttttgtc ataatcatcc tccagtcgaa attggagttt gctagaattt tcagagacgt
     4081 tctctactct tcttgctaaa aatcctgcag ctttccaacc gtgctttttc tcaacaattt
     4141 tgaaatttca cctttttata caaattattc aaaataattt gtgtatttta gtttaccata
     4201 aaacttaatc tttgactttg attatgtaaa agcttatttg tgttttgacc aaagagcgaa
     4261 ttccatagaa gaaaagctat aaaaaagttg acattaacat ttataaaagt taatatactt
     4321 actaaaagta acttaattac atataacaaa taaggtgaag aaaatgaact atcaaacaga
     4381 tgtgtgtata ataggagccg gaccaggcgg tgctctactg gcctatctac tggctaaaca
     4441 aaatatatca actatactga tagagagatc tgataaactt ggaaaagaat ttcgaggcga
     4501 gcatttaaat gaagatggag aaatgattct taagaaacat catatatttg aagaaattga
     4561 aagcttaggc ctccttcgaa tgtctagggt cgaatactgg aaggatggac atgtattcaa
     4621 aacgatagaa tctgaacttg gacatgcagg aattcatgtt ccacagcagc atcttcttca
     4681 gtctataaat aggcaagcaa gcctctatcc ctcctacaaa cttatgctgg gcacaaaagt
     4741 aacggaactc atgcagaatg aaaaaggaca gtatactgga ataaaagctg agcaaaaagg
     4801 ccggggtgaa ataatagttg aaagtaaaat cattattgga gcagatggtc gatattcaac
     4861 tgttcgcaaa cttgcgcaca taaaaaacaa tattcataat cctggatacg atttgctttg
     4921 ggcgaaaatt cctgcacctg agaaatggaa acctactatt cgctttacgg tagtgaacaa
     4981 tcagcaaatg gcactgttta cgcagacggg cggctttatt caaataggct ggaatattaa
     5041 aaaagggtcc tatacttccc taaagaagca gtctgtacag ccttttcttg atcagctaat
     5101 cctggcattt cctcaattaa aagaaagtat tgagcagaac cttcattcat ggaaaaattt
     5161 cattttattg tctgtttata gctctcactg tgatacttgg gtaaaagacg gattagctat
     5221 cattggcgac gccgcccata ccatgactcc aacgggcgca tatggtatga atgccgctat
     5281 aatggatgct gatgttcttt ctcagatttt acctaaatta ctttctcacg gttccttaga
     5341 agctggacaa ttaaaaaaat ttgaaaatgc acgaagaaaa atagtagaag atttgcagga
     5401 tcaacaagcg atgaccgaag cttcttttat agaaaatttt tcagctatct taaagattca
     5461 agaagaaagg cttcctcttt ttcaataaac taacaagaag ctacctcctt gtcgagcgta
     5521 gctttattgt taataaacct cataatttat aagcagactg ttcaaattca gcccaacact
     5581 tttatttttc tcattcagtt ttcctacttt taaatgttag tacgttgatt tcaggcgagt
     5641 caaagaaccg aaaattcaaa aatgggatgg ggccattgct tcctaaacct gcactaacgt
     5701 attgcttaat gtgcttgtat tcccacaggc ctttaactcg gtcttgagga ggaaacagac
     5761 cactcttttc ataccaagct tccggaacaa aaagtgcacc gaaaaaagga atacgaaact
     5821 gccctccatg ataatggccg gccataatta aatcataata cctaaatgta acgctgggat
     5881 ctctaagaag agcatcgatg tgagaatcaa ctactggaaa gtgattcaac ccaataacaa
     5941 ggtctgaggt ttctttttct ggatagccct tattgtttga taagtgactt ccgctggttt
     6001 ttgacttatt ctctgttttt aacgaccaat caaagttaac aaactgaata tgggcgtttc
     6061 ctctctttac tgtatacgtg gaatcaagca gcctcacacc tttactttcc atatctttaa
     6121 caaatttacc tttttcaaga ttacttgaag atgtacggta gcttttgggg tctgtatttc
     6181 catgtacaaa taaagcatgt tgtttatttt caataccttc tattaacgta taaaatggca
     6241 ccgcattttc actattattt ttcatcatgt cacctgtaaa aacaatagca tcataatgaa
     6301 tcgaattaat agtatttatg agtctttctt gattttctcc aaattctttt tcatgcaaat
     6361 ctgtaatttg aacaatagaa aagccttcaa gctctttagg taagtgctta attatgatgt
     6421 cttgcttagc tacagtaatt cgttgattat cccaaactgt atatccaatt aatacaacaa
     6481 ttaaaaagaa agaggctgct atcacttttc ctttcatctt tctcactagc atcccctcca
     6541 aggcattaag attaaaaact agcacaggct tcctcgtgag tcaaaatctc cataaatgcg
     6601 aatcttaaga ccatagaact tttaccacac tattacatct ctgtttatat agcataagta
     6661 aactataaaa atacgaatgt tgttttgacg tctcttttaa ctgagaagtt acttcaaacc
     6721 tattcggtaa taaccagctg cgcatttcac ttaaactatg ccaaacagaa tcatagctaa
     6781 aaaattgata gtccataaag gtaaaatatt tttttattcc tttcttttct tgttccaatg
     6841 ttgaagacgc aaaaccctta aattgatttt caatagaact taattctttt atttgttcca
     6901 atatgctgta ctggctatac acgatggaca atgttcgaat catcctctta tatgtttgat
     6961 gacgctttgc atccttaaag agagtttcat agcttatctt atcctttaca acatcaatca
     7021 tttgaataat atctaaaaaa tgcttaggcg aatctaagtt gtgcctccaa ccgtgaagac
     7081 atatcatata aaacgtatga tagtgggata aagcatttac aaactgaaaa ccttctaacc
     7141 ttactgcttc accccaaaac tcttggatgt taaaattagc cgttttttct tttaatatat
     7201 cccaatgaat ttcaactgtt agcggtatag ccgatccagg aatttctcta ctaaaactaa
     7261 cgtgaaagtg agaaggaata aattcttctt caattttaaa atccatagat tttactactt
     7321 ctattgcttt attgatgtct tttcttttaa ctaaaatatc gatatctgaa gtccctctag
     7381 cacctatgtg accaaaatat ttttcagcaa agtagactcc tttaagaggt atcacttcta
     7441 tctgttcttc ttcaaatttt cgaataatat gatccgtttg atttttaata aataaatttt
     7501 gatatagtgt ttcttgatat ttttgcttga gtcttgtttg aaaaaataaa ggagtatttc
     7561 ctagctctcc tttttctttc aataaggagt aaacttgtgg ggaaatgaaa tacaattcaa
     7621 tctcttttat tacttcataa taaaattcat catcacttgg tagattagcg ttcgaatcgt
     7681 aaaggtgtgt tataagttta tcaagcaaag cacttcttcc ttcctattat ttagctttca
     7741 gcatgattgg attctgttga ctgatttctt gtgcgttatt taatctgcgg tacattccac
     7801 tttgtctgat taactcttcg tgggtgccct tttgaacaac ttctccttga tccattacca
     7861 caatgaggtc tgcatgttga atagtagaca atcgatgggc aatcatgata gttgtacgat
     7921 ttatcattaa acgggccaat gcttctttta ccaaatgttc tgtttcactg tctagtgctg
     7981 aagttgcttc gtctaataga agaataggcg catcttttaa aatggctctt gcgatggcta
     8041 atcgctgtct ttgacctcct gacagcttag ctcctcgttc ccccacttct gtttcatagc
     8101 cgttacgcag cgtcataata tgatcatgaa tatttgcatg aacagcggca ttaatcattt
     8161 cttcttccgt agccaaaggt ctcgctatca ttagattatc ttttatcgta ccgctaaaaa
     8221 gaaatgtctc ttgaggaacg tatgcaattg aacttcttag ttctgaaatg gttaactcat
     8281 caattgaacg tccatcgatt gaaatagtgc caaaatgagg ttcataaaaa tgttgaatca
     8341 aattaaaaag cgttgatttt ccggcaccac ttggaccaac tagcgctact gctttaccag
     8401 caggaatgta taagttgaaa tccttgaata caatcgcgct ctcatcatag ctaaatgtaa
     8461 gatttctaac ctgtatatcc ttttgaacaa gcgttgcgga agtatagtga ggcagcgcct
     8521 tcgcttgagt tggataatta agtagtttag taattctttc taacgctgac acagaccgct
     8581 gatagccagc ccactgtcca gctagtcctg tcaagggaga aatcaactgg ttcacaaggt
     8641 taataaacgt taataatgca ccaatcgtaa taatcttgtc tgttacaaaa aaagcaccta
     8701 taattaagct aataagaaac gcaatggctc caattgcttc gcttccagcg taaaaccagc
     8761 ctcttaactt tgcctctctc atttccaatg aataaagctg ctcgttcttt tgtctataat
     8821 ctctataaaa cagtttttcc aatgtaaaag atcgaattac agtaaatccg tgaaacgtat
     8881 cattcagttt tttaataata ttgctaatta attcattcat gatacggcta ttatttcgaa
     8941 gtaaaatccc aaatatagct ccgccagcaa tcgcaatagg agatacgagc acaattgata
     9001 acgataattt ccaattaatt tgaactaaat aaataaaggc agcgatagaa ataatcggaa
     9061 atctaactaa attaattaaa cctcgcccaa ttacaccttc aacattatgt atatcgttag
     9121 tgaaatgaga caacaattca ccagaatgta aatttgaaac gtctcgttct cgtaaaagta
     9181 aaatatgttt atataaatcc aaattcaagt ctcgcttgac ggcattgacc gcaattgttt
     9241 caagatatgt atcaataaaa ttagctgtaa tacttgttac tgacaaccct acaccgatag
     9301 ctactagcca cttaagcctt tcaaaattac catgaacagc agcatcggta atgtttccga
     9361 taaaccatgc agatactaaa gtaagggtaa tttcaacaaa taacaaaata aacagtccaa
     9421 tataagcacc tttatgcttc attacgtatg gccagagcca ctgaaccata ctttttgtct
     9481 ctttgataga aatatattcg cggatgtcga attgctttat cttttttaaa ctaatcataa
     9541 cgacatcctc ctttcaatct gctttttcca ttcttgatag tttatatact ttcgtaacag
     9601 cactgacagg agaggaaaag caagaatgag ctttccccat aaaacagcaa ttacattttt
     9661 ttcttgaatc gccactgatt ttttttgaat aacaacaagt tttccgataa tcttttctcg
     9721 ggtaaccaga tcatccaccc cgtagttcgt atcaccttta aaaatataat attcttcttc
     9781 ttgaacgtag gaacagttta aaaggcgatg agcaatgagt ttattagctg gactttcaaa
     9841 taaaacaatg tctcctcttt ttagcggaga atgctttatt gaagtaaact ggcaaatatc
     9901 tccttgttta ataagaggat acatacttgt tccttcagca ggaagttcaa gcaatccatt
     9961 ccgatcaatc aaagctttta atagatgccc ctgcttatta ttagcttgca tacttttcaa
    10021 caaggccgca atcaattaac ttatttaaaa aatgctgtat atctccttca accatttcag
    10081 cttctgtatt gtaataagct gcaacagctt taataatttc cgcaagcgtt tttttgtttt
    10141 ctattacctt ccaacagtag cctcctactt cgtttaaagt agtcactgta taggcatgcg
    10201 aatttaaaat cacccactct gagtctactt ccattacttc ataaccgccc ttttgaatat
    10261 aacttgtcac gatgacatca actcccaaaa tgtattgttt ttttgaaaat gaagctcata
    10321 cacaggcact tgtttaacta acaattgtaa caggcccatg attgttttcg tttcttcagg
    10381 tttataagac cagtaaaaca ctttatctat cagcttcatc aacgcttcta ttctccttac
    10441 ctctatccgt tgattagata ttgactgatg cagtagctga atactttcta aaggtgtgtt
    10501 tttttcactt cccttggctt ttaactcact tctaaacggt gagttaaaaa ctgttacact
    10561 ctcgtctgta attcttaaaa ttgtagcttc atcagaaaga agttcccttg gcattgataa
    10621 ctttgctgca gtcgactttc cggctcctga gtgccctgta aaaatatgcg ccttgccgct
    10681 gttaataacg cacgatgaat ggagaagaat cccccagtta tgatgaacaa tgaacgaact
    10741 gtataagttc ataagagcat gttttaaagc caattcatcg tggatagcta cgttagccag
    10801 tttatactct gaatctactt caattaaata gtctgctctt ttaaaagtaa ttgtattgag
    10861 tttttcttct atagcgactt cataatcttg aaacgaaaca ccatagccat ttgtgattgt
    10921 gaccgtcatg tccggctgat ttttcgctat actaacacat aaaaaatatt gtttgattat
    10981 tttccatacg tcatcaaatt ctgttttaat atcaatatag tgatccccta tttttgtatg
    11041 cagcgtcttc attattcgtc tcctcaatca atttctctta aaacagcagt caatggaaca
    11101 ccattgactt tctgctttat atattgctgt attaagtaaa taaaatagct gaaaaaatat
    11161 tttatttatt tttccctttt ccttttcctg gggcacctgc accacctgga gtaggattgg
    11221 gatttgtaga cggccagtta gaaccattgt ttccttttcc aggatgatct aagtttcctt
    11281 ttccaggatt ccagctttga gcggtttcaa aacggattag ctgatgattt aacacttctg
    11341 gagcgctata agtcttctct tgcatatgat cacctccttt caattaattc tttattaaga
    11401 acgattgatt caaaaaattt aacccattgc tgtttagtaa agttctaatg agcagcttcc
    11461 atttgttttc ctagtaattg actaaatgta ttactctttt cttcggcaag ttgagtaaaa
    11521 ccaccttgtt gaataatttt accttgctcc agcacgataa cctgatcagc attgcgaatt
    11581 gtagatagac gatgagcaat aacaataatc gtcatttttc cttttaatcg ttccaatgct
    11641 tcttgaattt ttgcttcatt ttccgtgtca agcgcgctcg ttgcttcgtc taagactaaa
    11701 atagatggat tgcgtaaaat agcgcgggct agcacaagcc gctgcctttc tcctcctgaa
    11761 agcttaattc ctcggtctcc gattaatgaa tcaaggcctt gtggaagcct ccttacaaat
    11821 tctgcagccg atgaaaactc aagcgcttcc cacattcgct gttcacttgc gttaggttct
    11881 actaacagta aattttctct tatactcgca ttaaacaaaa aaggatcttg tggaacatag
    11941 ctaatagctc gtcttaacga taataaatta tcttttgata acggaatccc atcaattaaa
    12001 acctgacctt tttcaggctg attaagcccc atcaacaaat ctatcagcgt gcttttccca
    12061 gcaccggacc gacctacaaa agctgtcatt tgattagatg gaatgattaa attaatatgc
    12121 tgcaaagcgt aagatgcacc ttggttatag cgaaaataaa cgtttctgca ctgaatgtct
    12181 tttttaaatt gaactggttc gacactttga ttagcttctt tttcaaattc tcttgcattt
    12241 ttgcattcat cttgcaaggc tttcacagct ttaaatgctg gtagagttgt cgaaatctgt
    12301 tccatagagg cttgaatacc ggcaaccttt ggccataacc ttgaaaaaat tagaataata
    12361 agcattaact cacttgcctc agcatgaaac aacttcactg cagcatatat aaaggctgca
    12421 actaatacag cagatgagat tttataataa gattgtgacg ttgtatttag cttagtatat
    12481 tcaacttgct catttttcat ttcttctgta attgatcggt accagttcat tctcgattct
    12541 tctaaagtat tacttttaat atctttaatt ccgttaatct gatcggttat ccctgcaaga
    12601 tagctttttc ccagctcaaa gtttctattg cccaatgtca gcgatcgttt gagaaattta
    12661 cggtttaaaa agataatcac tacaccgcaa agcaatacaa aaagagttat tttaggtgaa
    12721 agccaaaatg ccagcccaat ttgtatgcac gtaaatataa gagaagctat aaactgcaaa
    12781 aacgaggttg ttcctccact agtacgagca atttctgtaa gtactatatt aataagatct
    12841 gactttcttt tttttataaa gaactcccag ttcgcatgaa gtaaggtgct ataagtctcc
    12901 atttgcatat gtctaagaaa accaagctga atgactctat tttgaatcgt tatctgtcgt
    12961 tgtaatatag tttgaccagt aactattaac acataaatac tcaaaattag aagcaaggtc
    13021 atagatacag gggtattttg taaaaatgaa aataccctta aaattgatgg tcctcctgta
    13081 tcaaaatcca cgataccact taagctaatc atcggaatta ataataaaat cccgatacct
    13141 tcaagtaaac tcataaacac cgttactaag aggttaatat agagcctttt gccagcaaac
    13201 gaatgtattt gcttcaagaa gtatacaagc tgtttcatta tgttctcccc ttacgtaact
    13261 catctttttt agtcttcctc caagcccata aaacaggacg caaaggaaaa tataagaaat
    13321 gaagtttttt aggcaaaggt aacatctctg catcttcagg gtaaggatgc aataaacttg
    13381 cggtaaagaa aagtttttgt tgagcagaca ttaatgagaa taaatggcga ctatgatatt
    13441 tggatacata ttcaggcacg gaatcagtat gtaaatttat catagtttca agataaaaga
    13501 ctgcctcttg agctagtttt tgggatcgtt tgcttattaa taaaggtttc attttttttt
    13561 ctaccctcac gtttaacaac tgtgaggcca aaataattgc ctgtccccct acataaggat
    13621 tatgataagt cgtaaataaa gtataaagtt ttacccaatc aatttttctt ttcactattt
    13681 gctgaatatc aatcagccag cgaaggcgag accagccatg acgcgctccg tgagccacaa
    13741 gaaattgaaa taggtcttct gtgcctaata agtacacagg ataactggta attgaacttt
    13801 tctcttttcg cttccatagt tcatcaaaac atggttcctt tccaggacct gggtgtaatc
    13861 tccaatgaat ttctagtttt atttctgtat gaggatgaat atatgtgaca tgatgatgcc
    13921 tccacttcca gtcatttaat acagtgtgaa tataatcatc cttttcatat ccaagactta
    13981 ctaaaaggtc ttctgctttc gcaagatctt gaattggaat aaggaaatct aaatcactgg
    14041 aagtacgaag agagatatct ccatacaaac atttagccaa aatgggacct tttaaaaata
    14101 gagtctgtat ttgatgttta gaaaagagca ggctaacttt ttccatttct gcacttaaac
    14161 gaagcatttg aaatgtattt cttttatatt gtctatttaa aaattgaact acataggttg
    14221 ggactacatc cgcatccaac atttttattt tgaaatttat cgctggataa acacgatgat
    14281 gcagcgtcag tttgataaaa aggttccagt caatatctcg acataatcct tcgctatatt
    14341 tttcaaggta ttgttcattc ccttccttca ataattcaag aattagtttt aattccttag
    14401 gcatgcggtc taagttgagt atgttatcca taattttctc cttcacgctt tttcacactg
    14461 atttcctttg caaacttagc tataacggta aatttcgctt taccttctgc accggaaata
    14521 aaaaatggac cgctgcgcaa ccatgcatgt gcaattaatt ttccgttttc atctttagca
    14581 gtacctaaat aaagtgtact ttcaatcttc cgtttttcta acatcttcat tcctgcaatt
    14641 gctttaacga gacattgact ttcccaaaaa gtgtaacggc tcataatatg aagagattgg
    14701 gctatatcct ttagaacttt atcatcggat gaactagtag tgaacgctgt ttccttcatt
    14761 tgctgtccta aggaaggaga tattttagaa aaaggtaacg ttttaagaaa acgggcccat
    14821 cctaaataaa taaatgcctc tatcaacaac agttttgctc tcatgttcaa ttttaaaaaa
    14881 accgttattt ttcgcaaaat tttcattttt ccctcccacg gataaaaaaa cattgaaact
    14941 aagagcgatt attctctgct acttgaatta aaccttcttt tatcagccca cttaaaaaag
    15001 caatgacttg ttcttcgcaa tcggattgtt cgacattata ttgagttact aatgtagaaa
    15061 ttaattcgct tactgaaatg ggttctttaa ttttctccca tatttctcca cctatttcac
    15121 ctaaattata gtacttacca ttgctgatac ttaacatcac tttttctcca tccatgtcac
    15181 tgacgatatg gcctgtttct tgactaacta attgattcat cgaaatgtct tgatttttaa
    15241 tcatttgttt tctctccttt ttattgtagt taaaattaag tctgttaaat cataagccgt
    15301 aaaaccagag attggtcgat gcaatcggta aaggtcaact ttattaacaa tttttgctga
    15361 tgcatcaaag tgccatttca ttaaaccaga gagttcaatg aaaaaattac ggtatgtatg
    15421 gttgaaaagc atatgaagcc tttctaattt ttcaatagac tgaattttaa tttcattact
    15481 gtcgttttta attaattcaa atataccagc aagaggataa gcttcggtgg caaactgaga
    15541 ttctactgga atagtaaact tggtttcccg gtcaaagatt ggacggtaac aagttgattc
    15601 cattccaagt tgagtcaagc tttcctgcca taatttttgt tgtggatacg ctggcgttac
    15661 aataggaata ttctcttcag taaacgttac gggtatgaca tcatcactta gaagccgata
    15721 tcctctcttt agaaaagcag aggcaagcgt tgattttcct gcacccgatt cacctactac
    15781 tgcataggcc tttccatcaa tagctatagc acttccatgt aacgggaaga tttttctttg
    15841 tattaataat gctcccatgc atgttcctag aatatatagc cgactcaagt cttcattaaa
    15901 gccctctgtc ggagaaaccg taatctcttt accatcttga attagataaa tagctaaacc
    15961 aggaacttta aacatacaca ggttttcttt cacgataaaa tacgcgcctg atttattttt
    16021 ttccgaccat agtgtggtta aatccccctt cttaactatg atttctactg atcctcccga
    16081 gatacttatt tcaggtagtt caggtaaagg aatttcactg agtaatttta atccaaatgc
    16141 ctcatacaca acctttttgg tagtatctat cattgagact cacctttgtt tgcaaataat
    16201 tttttcataa tagataccct cctgtacttt ttcaacaaca tagtcattac ctaataaaat
    16261 cacacactta agttcattat aaagaacaaa aagataaaaa aatactttta cgtgaatatg
    16321 ttagaaaaaa tcgcttagtt aaaacaaagt tattctttat aaagaacaat atctaaaata
    16381 aagttatcac cttttcttga tggtgtcaat cacttaaaat tactacctat atcttgagta
    16441 ataaatattc taaacaataa cttacttaac tgcctatttt atttatttca ttaggatatg
    16501 aaaatcagaa tttagcacct cactctaact tttaaaataa aaaccccaaa tacactatta
    16561 tgtataaggg gctttcaaaa attcgagaaa aatgaaacaa gagaggttaa tataagatca
    16621 aattttatat tttatttgtc tcaactcgta aaacgtttcc atgtaccagg gttatcttta
    16681 tcaacatctt tacctggtac atatgcttca tccgtataag gaccatagac tgtagaagcc
    16741 attgtcattg taatatctaa cgtttccaat acaggtattt gccactcttt cttcatcatc
    16801 tcacctcctc tcaattaaat ttttttataa agcgatgtac gattaaacta cgcatcatta
    16861 tcttataata aggattaatt gcatgctttg catgcacgcc ttcatgaata ttagacatcg
    16921 cttcttttat tcctttccca tctaaaaatt caagaattgt ttgatcttta ctaagttggt
    16981 gcagctcatc cgtaaattca ctccagtgcg gcatcatacg atggacccaa tccgcacctt
    17041 gaacaccacg aattcgttga tttaacctca ctgtatcagg taaaagcttt tctgttgctc
    17101 ttcgaatcag agctcgatcc attccttttt gaacatactg ttcctcaggc aaagacaagc
    17161 aaaaacgaat cactctaaga tcattggtgg gatcccgctt ccataatggg taacgcaaag
    17221 ataatttggt tgcaagtgta ttacttgcat tccaatgaaa aatatcttca aaatgccttt
    17281 ttctctgttc atatatattt gttgctgaaa accatcctgt ttcatctata ccgtagtttt
    17341 gcagcttatg aaaaacacct gttctttttg caaattctgg gttaatcagc atcggaaact
    17401 catatgaatc tcctagtgga aaaagtcgct caataaaagg aaacgctact cttgcaataa
    17461 ctgggattct tcgaaatcta cttcctccag ctttgtggct atagtggtga agttcgctag
    17521 ctaatcgaat ccattttaat ttttttaaaa gaagagcata gtaatcaata gctgaacccc
    17581 aggatatgct taaattcccc ctaccgccat ttagcagcac tcctacattt tcctcactag
    17641 ccttctcaaa catccctttt aaccagaaag aattttcaaa aaatttataa ggcatttcca
    17701 tagtgttgag aaaatcatca atttccgaat aggaatctct tccttcaaaa tctaaataat
    17761 gatctttgat accacctata tgctgtacag ttgattgaat gaagggcgtt tcgtttggca
    17821 ttaagtgttt aggtgtaaaa tctttaaaat cattaggagg aacatagcta aatgtatata
    17881 gttgcttacg tgtcccacta agactttttg cagcaaaact aacaattgaa cctgagtcta
    17941 aacctccact caactgagct ccaacttgac gataagtgcg taaacgtgaa gtaactgcct
    18001 cttgaaacac ttcttgaaac gcttctatat actcttcatt agatttcaat tttaatgttt
    18061 ggacggaagt taatgtacaa tattgcttaa cctttactgt ttcttccatt acggtaatac
    18121 tgtgtgcagg gggcatctgt gctatatttt tataaggggt tagtgatgcg tctactacat
    18181 caaccattcc agaaattgcc aaaaattccg caagccactg ttcatttaat ttcttttcta
    18241 catagggaag ggataacaat ggttctatag tagtgcagaa agcaaatcta tttgtatcat
    18301 gataaaagta aagggttcta cttcctgaaa aatctctcgc accaaagagt atttgctttc
    18361 tctcatccca aattacaaaa gcaaaatcac ctactaaata ctttggggac tcctctcccc
    18421 acttgtaata ggcaagtaag attaattgac tatccgtcat cgcttttctt tcaatgtatt
    18481 taacctgcag tttctcgaat aattcatctc gattgtcgat aatgacatca gctgtaattg
    18541 ctagctgcct ttcataatca caataaggct gctgctctcc cactgattcc ggtgtaatcc
    18601 attgtgcatg gcaaccaaga aagatatttt tcttatgcga tatttgaata tcattagcag
    18661 gatatttttg taaagattgc ataactctat tcacatgctc gacaggtaca ggttcattat
    18721 tagaatgata aataccagca attgcactca tgattttctc cctttcaaat atgtatcatg
    18781 atagaactta ttctttttaa agaactaaga tttaaaataa tttactttta tatcttaaat
    18841 cgttttaatt taactttata aattccaaat caatttctct atccaaccta tattgttctt
    18901 tataaagaac aatataggta aattatatct tatttacatc agatgtcaat gactcagaag
    18961 aaaatttcaa atgatatttt aattaacaca tgaattcacc ataatttcac aataattacc
    19021 gtttagtcca agcaataaga ggaaatttcg aatttaatat tataaggaaa aaataaagat
    19081 gagcatctct ttttattaaa gctataacaa ttaacggggg gatttgcatt ggcatataag
    19141 atacaaatca gcagtagttt aaagaaggat ttaccaactt taggtattgg ggaatttgga
    19201 ttatgcacag atacagccga attatttatc ggttctgatt ctggtaatat ccaaatagca
    19261 aataaaagtg aaattgggct tttatcaaat ttaacaacta tcgataaaga aaatttagtt
    19321 aatgcaatta atgaaataga aagaaaaatt agtgacaatc acggagccac tagtttagat
    19381 gagttgagtg acgtagatat atctacatta tcacctaaaa atggggatgt tctcaaatat
    19441 gaagccggca tttggaaacc agcaacaaat ttatcaggta caatatcctc atctccttat
    19501 gtaattgaac tcaataggtg gaatattaaa aacgatggaa cttcttcgaa aactactacc
    19561 cagggtatca atgatgcaat tatttgggct aagtctcaag gcgttaacca tgtaatatta
    19621 cctagtggtt tttataaact aaagctaaaa ccatgggtat ggcaagatgc tgcttcatta
    19681 tccttcacat gtattaatat ggtaaatgac cttcactttg aaatggcaga tggttgcata
    19741 ctagaattag aaggtaacag ttctccaggc tacagtattt ttcaagttaa agatgtcaaa
    19801 aacgtaaaga tttctggcgg taagataatc ggagaccgaa aaacacatta ttacgaaatt
    19861 ggtgtgaaat ttgttcgggg tggaatcaac cctgatggat cattaaacga taatcttaat
    19921 ttcataagaa gtgaaataat tgatagattt aaggatccgg gacttttaaa caattttagg
    19981 ctatggaaaa tcgaagggat agatgtatct aaaggctata gcttttatca gtacaaagac
    20041 acagtttcta ataacacatt agttggtttt agaaataatg gacaatttgc tccatcatca
    20101 ccaacaggca gaggatggct cttacagcct aatattggag caaacaacaa aatggttttc
    20161 actattgata ttactaatac cccattaacg gataatcaaa ttgcggctat tactgcgaaa
    20221 gttgataatg aaagatatac tcatgaatgg ggacaaggaa tcgaaatagc aggttcaaat
    20281 catgtagaaa ttaaaaacac agagatatgt gattgtactg gagatgctgt ttcaacaggt
    20341 tggttacaat accgaactaa ttcatctgac tatactcaga acgaaatggg acaccacatt
    20401 tgggttcatg gctgtgatat acaccactgt agaagacaag gtattactct tgcttcagca
    20461 aacgatgtat acatttatga taataagatt catcatatag gtaaagcaga tgataatgtt
    20521 acatcagact ttcgcaatgg aattcctcct atgtttgcaa tagatattga gtctatggta
    20581 ggtgaaagca acataccata taaacagcct tactataata aagatggtct agagctgaac
    20641 tttaggttat acatttttaa caatcatatt cacgataatg aaagaggcca ctttgtgaac
    20701 gcagatggta accatgtaac cgtagaaaat aatattttcg aaggttggaa tgttggagga
    20761 gttagttctt atcctacaaa catgtatata aagtatttaa ataatacttt tattaataca
    20821 gaatttattg tatctggtga caattatgta aatggcttag ttggtcaaca gtctaatctt
    20881 agattaatgg acgttagagg ttctgttatt caagacatac gtttaaataa tggcattttt
    20941 tatggaaatg ccaactatgg ttattttggt acgcctacag ttgatgtttc aacgagttct
    21001 tttattatca ataatcacgg tatgggaaat acggctaaaa tttgttttga acaatgggta
    21061 ggaaaggttc ctagcagtat tagtgtcgac aaaatatact atgttgtaaa tcgtactact
    21121 aattcttttc aagtatctga aactgaagat ggccctcctg tcacctttta tgattccggc
    21181 gagtggggat ttaatgttag tcgatttaat tatggaaggt gctatattaa taatgttgtt
    21241 attgaaagag actggaatag tggtggaaca acacccggct ttgcagtaac tgcagcaggt
    21301 agcattattc agaatgtcac agttaaaaat catagtgtaa gtattaatgg aaacggatct
    21361 aatatttatg taggcaggcc gttaacagtt gacggtttaa ctattataga cggtagtgcg
    21421 aatattaaag attgtaatgt attaaatagt caattcatca gaaatagaaa caagtcaatt
    21481 agtcctgact taaaatttgg tgattcagct ttcccagaaa caaaagttaa tattaaaaac
    21541 agcctattta gaaatatcga tgcaacttta ataaattcta ctgtagataa ttgttcattt
    21601 tttaacgtaa agctcagtaa aactgataac aacacccaaa caattttgaa ccattgctat
    21661 ttagaaaatt ctatagttga tgggtactgg ctagaactaa aaaaaggata tttaattaca
    21721 agatgtaccc ttcttaatac agctatgact gtaaataaaa atcctggaac aaccttgtta
    21781 ttagaaaaca ttgatatcac taattccaca gctgacctta atgcacctag ggtaactgtt
    21841 aaccctaatg ggggaatata taatacagat aaaacggtaa cattattagc aaatgaagat
    21901 gcaattattt actatacctt agacggaacg cttccaacta catctagtat tcaatattct
    21961 acgcccctta aactttcaaa tcccactacc ctaaaagtta ttgcagtaga tgctgctggt
    22021 aatcaaagta aagttaaatc agttcagttt aatatagata aaactcctcc aaccaatgtt
    22081 acaaatttaa ctgctgcacc gtttaatgat aaagtagatt tatcctggac agctagtact
    22141 tctaacgatg tattaaatta tgaagtatat aacggggata cacttataag gtccattaac
    22201 agtactacgt atactgttaa tggtcttcta gctgcaagtc aatattcatt tactgttaaa
    22261 acggtagaca aagcaggaaa taaatcatct ggtatttcta tcatagttac tacttcacaa
    22321 gataccacac cgccagaaga gatttcaaac ttattagcaa gcagtatcac ttcagactcg
    22381 attggattaa cttggactcg aagcttatcc tctgatgttg caagttacga aatataccag
    22441 gactctgtct tattaaatgg aaatgttaca actacgaatt acagtataac tggattaaaa
    22501 gcaaatatac aatataagtt aactgtaaaa tcaaaagata taaacggaaa tgtttctgat
    22561 ggtattagtg ttacagtaga gacttcagcc gtatcagcta actacgtaac aaatggcctt
    22621 attttatata aagataatcc ttccaacaat ttaattattg aaaatccgga cagctatttt
    22681 atggtgaatg atacatttac cgtttccttt actgcaaaag tacctaaatt tggtgactta
    22741 attagcagat atgaattggg tggatctcaa aataaaatat tgttagaacg caatggaaat
    22801 aatgcttttg ggttttctat atggggaagt accgctaaca caaccgttac ctctcctcag
    22861 tatgcagatg aatctttgta ttatcacatt gatgtaataa gaaccggcac agcattgtta
    22921 atgtatatca ataatgttaa agtctcggaa gcaggaatag ctaataacgt ttatactaaa
    22981 ccttcctccc tcccgctcat tataggcgat aaagcagtac cagttttatt taaaaatgta
    23041 gcatattata atagaatatt aactcctgaa gaattagccc ttaactttaa tgcgcttaag
    23101 taatatgatt acatttttaa tttccctaaa ataataattc aaaaagacta gaataccttt
    23161 aataattaaa tcattctagt cttctatatt tctgcaatta tttctttact ctttactctt
    23221 ttctttttaa ttttgccact ttctctacaa accattattt agatacaatc taaatttgaa
    23281 gttttattac ttttcttgat tttaatatat tcctcatttt tcatttgcaa taatgagtac
    23341 tcttttatct tattgttttt atttattaca tactataatg atgaaattat tattaccgct
    23401 aaaactaaca tttaactttt ataagtaact tttttattta ttttaaatcg ttctaatatc
    23461 gttcccatta tgtttttaat tatattaaat aaatatttga actccttggt tttgtaaaaa
    23521 ctaatatata tatcgtattg atagttatta aacagataat tccctttaaa actaatgtta
    23581 cgaaagtgtc gggttctata agactccaca ctaaatttgt cgtaaaatag gttcctgtag
    23641 ctataattgt aaagtaaaaa tatcttacga aatacattat tatgggttta ttaaatactt
    23701 ttttatagac taaataaggt gttacccaaa aaggcacgac tagggcacta ataagagttc
    23761 cgataaaaac acctgcaatg cctatgtgct gaaccacaat aattgaaata actagattaa
    23821 cagctgcctg aaataaaggg gcaaacctgt cttcatgaaa aattcctgaa gttgttttca
    23881 ctgtagaaat tgaatttctc atacctcttt cataaaaaat aataattagt attattacta
    23941 cacctttact cattaaaaat ttggatccta tccaaatagc aatgaacggt tctaacatga
    24001 ttagtagcaa aatacccaga gttgaatata gccagaagct caataacata gttactttat
    24061 aaatgctata aaccttctct ttagtttctt gtgctattaa atttccaacg ctatgataaa
    24121 tattactaaa aacttgattt acaaaagtcc tacaaatttc tattagcata ttataattag
    24181 aatacaaccc aactgctgtg acacttacaa atgtagatat aataatattg tcagtaccaa
    24241 acacaagata ggttccaata ttttgcaaga caatagcctt tatattttta ataatgttgc
    24301 ttctagtttc gctgtctaat tttctggtta ctttattttt caaaaatgga tacattttat
    24361 ttacaataat ggctaaaaca gcagatgtaa ctattgttat aaaacaatct gcaactaagt
    24421 ataaaataaa gtttttagta taatataata tacctatttt aaggcaaaca gatgcaatcg
    24481 aagatataga gtagattcct gttatcatat atgttttttg acatacgctt aaaagttgtt
    24541 tttgtgtaag tacaagtatg gtaaaacagt attaattaaa aagattagat agattaaatg
    24601 aatgttttct acactagtat ccttagtaaa aagttcaaga aatggcatga tcgataagcc
    24661 caccagtaat acaattaagg caatcaccat atatgctttc ttatataagt tcattagaac
    24721 atttattttt tcctgatcat tttctgctac tggtttataa aggctataaa caatacttga
    24781 gcctacaccc gcttcagcta atgctaacat tgctaatata ctagtaaaga gcccactgat
    24841 tcctaagtat tcgaccccta aatatgttat aaataccgtt cttgaaataa agcttaatga
    24901 tgtaataata atttgattcc ctaaacccgt tgctatgtta ataattgaat gtttagttct
    24961 catttgctct cctttaggtc acatgtataa gaaatataaa atttatactc tcctgtatat
    25021 ttaacaacct atttaataaa aactatttca tctttttagc tgttcatgca ttttattctg
    25081 actgatacgt gatacatttt cttttatagt ataattactt tcttatatat tagctttagt
    25141 atcattaaat ttttagggat tttttctagg ttacgtttcc tgttaaagaa ttcgttacta
    25201 tgatcctata aattccatgt aataactatt gaatttttat tattagttac ttctgatata
    25261 attaaagctc tagcaccgct aaatacactt gaggtgctgg aaggacattg tatatgagag
    25321 taaacaaata ttattcccgt cttaattaac caattatcac caagtttcat tcctgtttca
    25381 ataagatttt ctattcgcat tttacttctt tgtaatagat atagcattta tcattcttat
    25441 gcaaaatcct tgcgtgaatg gcagtatatt gaataacatt gcatacaccc ctatatcttt
    25501 tgtatctcct tgagaaaaat caacttctcc agttcttctt gtaacgctca tcagataatt
    25561 tatggctttg gtagtgctgt ctagacattc tttggaaatc ccatcaattt ttgaagcatt
    25621 aattaaaaac cagcctaagg tggcggtggt agaagaatcc gctctagatt catttctagt
    25681 gacagtccag ttccagcttc cgttatcctg ctgaaagctc atagcagctt tagcgaattt
    25741 tttcacactt tcttctagta tcgatttatg tctactatcg gttggcagtt cggcccatga
    25801 atctataaga ccgatagcat accagccaag acctcttccc caaccgtata atcctaaggg
    25861 tattttatta tctaccttat aagcgtgaca cgggatagag tatatttcta acatgccata
    25921 tcgttcgtac tctttaattt gttttactgc taattctatg cattcacctt tattatattt
    25981 tgttccataa gcaattaaaa aaggacatat gaaacctatc gtatctacat atctgtagct
    26041 cggcatagat ttcctatatt caactgtgcc ttcttcgcct atatgctctt ttatcatttc
    26101 ccatgtataa tccaatgctt ttctgtacgt atcagtttct ataaagtcca atttcatgac
    26161 agcataagcc aatattgccc catctacatg tacaggcttc tttatccatt gacctgcact
    26221 atcaaacttt ttgtttaaaa actttactat ctcattttca gctttgtgat cttcaatact
    26281 ttttaaatat tctgataaac ctaataatat tgatgcctct tgccagtgct gtatagcact
    26341 tttagtataa tttcctttta acatatctaa tacaattaag cgtgtattat cggttacctt
    26401 tatctttggg gtatcattta accaactaac tgctttctca gtaattaatt tattccagat
    26461 gtttttattg tcatatctcc ctatgtgaat ccgccccaac cagtctttaa atataggaat
    26521 aatatctatt agtataacta ttactattag agtaattaat aaaaaacact tgtttgaata
    26581 atcatttcta tgtcctttcc tatcctgcat taaacaattt atataacttt tctatttcag
    26641 actcagttcc cagcttttct tttgacaaat tgtttgttaa ttttcctctt aaatgatcat
    26701 catcaagcaa tcttttgatt ccctcagcaa ttgatgatga tgtcatatct acaattaatc
    26761 cagtttcctg atctttaatt tgatctttaa ctgtagtaaa gttagttact acaattggtt
    26821 tattaagtat ctttgcttca tcaatagcta tagatttacc ttcaaatctg gaaggatgaa
    26881 cataaatatc tgcatttttt atgtatggat atgggttttc tttaagacct aacagtataa
    26941 agttctcttc aagcctattg gctttaatta gcttttctag cttagttctt tcctcccctt
    27001 ctccaattac atgccacttt attttataac cgctatcaat taaattctta catgcttcta
    27061 tcgccagctc aaaccctttt tgttgatgta acctgcctaa tgaaactaaa tttactccac
    27121 ggtattttat gttcaaatgg ttcgatgaca ttttatttat tactttcggt gaaacaatat
    27181 tatacatcac ttcaactttg tctttgtata caggaaatcg ttgttttaaa acactagcac
    27241 attcttcaga tactgtaaca atgttgccca gtttatcgaa atatttttga tcaatttcag
    27301 ggtccattcc aagtttgtcg taatcattat gaataaatcc tattttcttt tttgcattta
    27361 ctttgtctac acaaaagtaa atgggggttt tttccaggta accaattgca acatcatact
    27421 tcttatttat actttttaaa gaattacata tatatttcca gactctttgt tcacacctaa
    27481 ctctgttttg ttcagtttta aatatgaaac cagcttttaa ccttgataaa gctatgttaa
    27541 gtctgccttt cttaatacaa cttattattg ctctttttat aggcatgtca taatatttat
    27601 attcataagg ctcttttaat aaatttactt gtttaggtat tttcgaaaaa ataaaccttc
    27661 atgtttaaat aaaaataagt ccacattaaa cttagagtaa tctatggttt ctaacaaaga
    27721 aataagagcc ttttcggcac ccccacagct taaattattc attacaaata atagattttt
    27781 ttcattaata gtcaccctct ataatatttc atacaactta tttatttctt cttctgtacc
    27841 taattcttct ttagataaat tacttattat ggtttttttg agttcaaaat cttttataag
    27901 ttgttcaatt ccttcagaaa tagctattga attcatatct actattagtc cattgacacc
    27961 gtctgttatt tggtctctag ctgtgctgaa attagtaaca ataataggtt tttgaaggat
    28021 cttagcttca tctatagcta tagatttacc ttcaaacctt gatgtttgtg catatatatc
    28081 ggcttgtttt atgtagggat atgggttaga ttttaatcct aacaatataa catgattttc
    28141 cagattattt attttaatta actttgtaag attctctctc tcttcgccat cacctataat
    28201 gttccatttt atattaaaac ctttatctac taattttcta catgctttaa tagccaactc
    28261 aaatcctttt tgatagtgta gccttcctat agacaaaatg acaatctcat tacatttttt
    28321 gtcataaaca cccattttaa cttgtttagc catcttattt atcaattttg gtgaaacgat
    28381 attgtacatg attcttactt tattgcgttc actagggaaa cgacttttca gaatattacc
    28441 acattcttct gagacggtaa taatattatt gagctttcta aaatatcgta tatcaaattt
    28501 tggatccata cctaatttgt cataatcaat atgaatccaa cctatttttt taaagcgtct
    28561 accttatcta tacaaaagta ggtagatgtt ttctctaaaa acctatagct acatcatatt
    28621 tttatcaatt ttttctaaag attcagaaat atacttccag ccatattgtt ctttttgaga
    28681 aatgttttta ttatttttat ttattaatgc aaacatgatc ctattaaata gtaaagatat
    28741 tttacctttt a
//
