LOCUS       NZ_CP063142            22142 bp    DNA     circular CON 30-MAR-2024
DEFINITION  Priestia filamentosa strain GN1 chromosome.
ACCESSION   NZ_CP063142
VERSION     NZ_CP063142.1
KEYWORDS    RefSeq.
SOURCE      Priestia filamentosa
  ORGANISM  Priestia filamentosa
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Priestia.
REFERENCE   1  (bases 1 to 5133640)
  AUTHORS   Buch,A., Patel,D. and Chandnani,M.
  TITLE     HCA producing B. filamentosus GN1
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5133640)
  AUTHORS   Buch,A., Patel,D. and Chandnani,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAY-2020) Department of Biological Sciences, P D Patel
            Institute of Applied Sciences, Charotar University of Science and
            Technology, Changa, Anand, Gujarat 388421, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. v-3.13.0
            Assembly Name                     :: CHAR_BFil_1.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina Nextseq500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022811745.1-RS_2024_03_29
            Annotation Date                   :: 03/29/2024 08:14:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,159
            CDSs (total)                      :: 5,093
            Genes (coding)                    :: 5,023
            CDSs (with protein)               :: 5,023
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 21 of 70
            Pseudo Genes (incomplete)         :: 52 of 70
            Pseudo Genes (internal stop)      :: 15 of 70
            Pseudo Genes (multiple problems)  :: 15 of 70
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 10:46:23
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2826616
            Orig. end                         :: 2848758
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP063142.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Dr Aditi Buch, Department of
            Biological Sciences, P D Patel Institute of Applied Sciences,
            CHARUSAT.
FEATURES             Location/Qualifiers
     region          1..22142
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="opine-like-metallophore"
                     /region_number="9"
                     /rules="(CntL and CntM)"
                     /tool="antismash"
     cand_cluster    1..22142
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(CntL and CntM)"
                     /kind="single"
                     /product="opine-like-metallophore"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..22142
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[10000:12142](-)"
                     /cutoff="5000"
                     /detection_rule="(CntL and CntM)"
                     /neighbourhood="10000"
                     /product="opine-like-metallophore"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..12142)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="5000"
                     /detection_rule="(CntL and CntM)"
                     /neighbourhood="10000"
                     /product="opine-like-metallophore"
                     /protocluster_number="1"
     gene            complement(594..1046)
                     /locus_tag="HPB58_RS14195"
                     /old_locus_tag="HPB58_14150"
     CDS             complement(594..1046)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019394401.1"
                     /locus_tag="HPB58_RS14195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HPB58_14150"
                     /product="DUF2383 domain-containing protein"
                     /protein_id="WP_243509631.1"
                     /transl_table=11
                     /translation="MDKKQIVDTLNKLVEGQYMGIHAYENFIKNLDSSSSLLSHFIEIR
                     KDLEHHAELLSKRVQILDGTPKSSEGIIGKIELFISQLFDSSTSEQDILKHAIKGQNLY
                     GIKMTEELVSNKLDEERLCLVHEILQKQREHVDYLKYELHSRTSSQ"
     misc_feature    1092..1107
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 23.45"
                     /tool="antismash"
     gene            complement(1416..2099)
                     /locus_tag="HPB58_RS14200"
                     /old_locus_tag="HPB58_14155"
     CDS             complement(1416..2099)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019394402.1"
                     /locus_tag="HPB58_RS14200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HPB58_14155"
                     /product="DUF554 domain-containing protein"
                     /protein_id="WP_243509633.1"
                     /transl_table=11
                     /translation="MFGTIFNTLTIIVGSVIGSIFKKGIKHEYHNILMQAMGLVATVLG
                     IDAVVHHLPSSKYPVLFIVSLAIGGLIGQKLNLEVRFNNLVNKFSKSNLAEGLSTAILL
                     FCIGSLSILGPIEAALKEDYTYLLANGILDGITSIVLASTFGIGIAIAAIVLFIWQGSI
                     YLITILLEGAINHSLLNELSIVGGILILASGLNILEIKKFKTLNLIPALLVSPVLFLII
                     ELFPF"
     gene            complement(2207..3025)
                     /locus_tag="HPB58_RS14205"
                     /old_locus_tag="HPB58_14160"
     CDS             complement(2207..3025)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1171:
                     transcriptional regulator, MerR family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019394403.1"
                     /locus_tag="HPB58_RS14205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HPB58_14160"
                     /product="MerR family transcriptional regulator"
                     /protein_id="WP_243509635.1"
                     /transl_table=11
                     /translation="MKELFTIGEISKLFHMKISTLRYYDEIGLLRPQFTDDKTHYRYYS
                     TQQFERLDSIKYLRALGVPISELLEFFNYRDIDNLIVMLKNQQAEIAQKKIELETIERK
                     ISQRLLQINHAINTPLDTIAEVKLPGRHVAYLQHDYSPSEDIEFPISELRTNFNLKTNI
                     FLGKIGLSISVSDLCAGTFNKYSSVFMTVEEDDEVASPTTVFPPREYLQICFKGTHMDA
                     PPYYKKLMEYIRDHQYKLIDDSMEITLIDYGVTNDLNKYVTEILLPYSRL"
     gene            complement(3472..4659)
                     /locus_tag="HPB58_RS14215"
                     /old_locus_tag="HPB58_14170"
     CDS             complement(3472..4659)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1202: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016098180.1"
                     /locus_tag="HPB58_RS14215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HPB58_14170"
                     /product="MFS transporter"
                     /protein_id="WP_243509637.1"
                     /transl_table=11
                     /translation="MNGAMSWPFLRLYGLTLLYFSANSILNVIIPLQGETLGASNTTIG
                     IIMGAYLFTTMFFRPWAGHIIQKKGPIKVLRIILIINGFALILYTITGLGGFLIARILQ
                     GVSTAFFSMALQIGIIDALPEEDRSQGISLYSLFSHIPGVIGPILALGIWQANNTAYFT
                     IVMISIAIFTGFFGYSAKVDKREDLTTINNTHQRVNMFKSFGELIKNPFLFKCSVLMLV
                     ASIVFGAITTFLPLYAMELKNGSAGVYLMIQAGTVVFARFTLRKKIPSDGKWHAPFVMG
                     TMLLLALAAQCVSFSITGGAMFFYAGAVLMGIAQALIYPTLTTYLTFVLPKVNRNILIG
                     LFIAMADLGVSLGGVIMGPIADISSYSFMYRICAILGGIMIVFAYDKRKAFVEGK"
     misc_feature    4655..4670
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: medium, score: 23.56"
                     /tool="antismash"
     misc_feature    4725..4740
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: weak, score: 20.6"
                     /tool="antismash"
     gene            complement(4741..5487)
                     /locus_tag="HPB58_RS14220"
                     /old_locus_tag="HPB58_14175"
     CDS             complement(4741..5487)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015735103.1"
                     /locus_tag="HPB58_RS14220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HPB58_14175"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_243509639.1"
                     /transl_table=11
                     /translation="MLIVESVEKVYKTGRLFSKEKRKILKNISFECKSGECIGIIGESG
                     SGKSTLGRLLIGIEQPDAGRILFEGRSMNDRLTRLGSISAVFQDYKSSINPFFTVEQAI
                     IEPIRVKKSNKIEIESRIDYLLNQVGLDSSYKEKYPHELSGGQVQRVCIARAIATEPKC
                     ILLDEAISSLDVSVQVQILKLLKELKEIYRIGYIFITHDIQAAAYICDRIIIFKNGKIE
                     ETVETSNLKNVQSEYARELLQKLITF"
     gene            complement(5481..6296)
                     /locus_tag="HPB58_RS14225"
                     /old_locus_tag="HPB58_14180"
     CDS             complement(5481..6296)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006638074.1"
                     /locus_tag="HPB58_RS14225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HPB58_14180"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_243509641.1"
                     /transl_table=11
                     /translation="MNILEVENLRIQDIKNGKVIIHNSSFSVRQGSCLAIVGESGSGKS
                     VTCRSIMRLNRPSLSQSGTILFKGENLNTLTEKEMRKKRGKNLCMIPQNGMSAFDPSCV
                     VGMHLKETLFEHFGWNNKEIERKMRSTMESVMLKNPIEIMNKYPHQLSGGMLQRLMIAL
                     AIALEPDIIIADEPTTALDTISQFEVVEQFIKLREKIGCSMIFISHDLGVVKKVGDDIL
                     IMKDGRIVERGSIQTIFSQAQHEYTNYLLSTKQVLSNHFKKVMGMNNKC"
     gene            complement(6301..7197)
                     /gene="opp1C"
                     /locus_tag="HPB58_RS14230"
                     /old_locus_tag="HPB58_14185"
     CDS             complement(6301..7197)
                     /GO_component="GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="opp1C"
                     /gene_functions="transport (smcogs) SMCOG1085:
                     binding-protein-dependent transport systems"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016098177.1"
                     /locus_tag="HPB58_RS14230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HPB58_14185"
                     /product="nickel/cobalt ABC transporter permease"
                     /protein_id="WP_243509643.1"
                     /transl_table=11
                     /translation="MRILKNLSKDKLATVSLIIIILTMVIGIFSPIFAPHNPKEVNIGL
                     RYAAPSWEYLLGNDHLGRCILSRLIYGIRPSVLWVSVALFLSVVIGSVLGFLAGYFRGK
                     VDGVIMGICDIMLSFPGYVMTLAIVGILGVGLVNILIAFIFLKWAWFTRVIRTSVMQYS
                     ELEYVKFAKACGISNMRIIYKHIVPVTLSDVIVLSSSSVGSMVLQISGLSFLGLGIQAP
                     NAEWGMMLSEAKEVMFTRPELMLAPGLAIIIVVSAFNFLSDALQVALDPKLMSSKNRIK
                     GRLVSPRHKIQKKEVAK"
     gene            complement(7198..8130)
                     /gene="opp1B"
                     /locus_tag="HPB58_RS14235"
                     /old_locus_tag="HPB58_14190"
     CDS             complement(7198..8130)
                     /GO_component="GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="opp1B"
                     /gene_functions="transport (smcogs) SMCOG1118:
                     binding-protein-dependent transport systems"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014650326.1"
                     /locus_tag="HPB58_RS14235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HPB58_14190"
                     /product="nickel/cobalt ABC transporter permease"
                     /protein_id="WP_243509646.1"
                     /transl_table=11
                     /translation="MGSYIIKKLLISIPLLLVISFLTFILINLSPQDPAEVVLQAQGVP
                     KITDELIMETKQELGLNQPFIMQYLHWVIACFQLDFGNSYVTGQSVWSLIGPAFLNTLK
                     LTSVSSVFVIVLSIFLGVLCAMREGRLFDKSVRGVSFFLTSAPAYWLASMIIWYFSIKL
                     DLLPTSGMSSYKSYILPVIVITISYAGIYFRIVRSSMLNNLNEDYVLYGRACGLNEQTI
                     TLQILRNSLQVSISVFCMAIPIILGSTVVIENVFAWPGLGSLSVKSILSRDFPVIQAYV
                     LLLAVSFVIFNTISDIINATINPKLRNDL"
     gene            complement(8235..9812)
                     /gene="nikA"
                     /locus_tag="HPB58_RS14240"
                     /old_locus_tag="HPB58_14195"
     CDS             complement(8235..9812)
                     /EC_number="7.2.2.-"
                     /GO_component="GO:0009897 - external side of plasma
                     membrane [Evidence IEA]"
                     /GO_component="GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /GO_function="GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA]"
                     /GO_function="GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /GO_process="GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="nikA"
                     /gene_functions="transport (smcogs) SMCOG1068:
                     extracellular solute-binding protein family 5"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019535234.1"
                     /locus_tag="HPB58_RS14240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HPB58_14195"
                     /product="nickel ABC transporter substrate-binding protein"
                     /protein_id="WP_243520852.1"
                     /transl_table=11
                     /translation="MVVVCLVVLNTSERKKTAETDSKSNEKGIVYAASKDINDMNPHLY
                     TGSMPAQAIVYESLVENTEDGIKPLLAESWNISKDGKTYTFHLRKDVKFHDGEPFNAEA
                     VKKNIDAVQANAEKHSWIKLSTKIVDCKVIDEYTVELVLSEAYYPTLVELSMTRPYVFI
                     SPKDFINGETKGGVRGYHGTGPYVLTEHKVDEYATFKANENYWNGVPKVKKITSKVLPA
                     GETTFLALQKGEVNFAFTDDRGTDSLDVEAMDQLVDLGDYQLIRSEPMNTKMIVANSSQ
                     SGNPVHEKPVREAIWYAIDRKTISKEILSGTETVAHTLFSHNINYADVNLKKRGYDLNK
                     AKGILQKAGWAIEDSNGIRIRDGEKLAVKLYYDSSSSSQKTQAEFIQSSLKEIGVQLQI
                     IGEESTSIANRRSTGDYDLLFNQTWGLAYDPQSTISAFTSPSSYLHTTKGIEKADELYN
                     KIDDVMVSMNEETRKSLYADILKIVHDEAVFIPISNGSVTVIAPKNLKGILFKQTQFEL
                     PFEKMHFE"
     gene            complement(10001..11311)
                     /locus_tag="HPB58_RS14245"
                     /old_locus_tag="HPB58_14200"
     CDS             complement(10001..11311)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     opine-like-metallophore: CntM"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015891119.1"
                     /locus_tag="HPB58_RS14245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HPB58_14200"
                     /product="opine metallophore biosynthesis dehydrogenase"
                     /protein_id="WP_243509648.1"
                     /sec_met_domain="CntM (E-value: 1.3e-186, bitscore: 618.8,
                     seeds: 4, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDFKRILILGTGPTSIQLAVSLKKQSNCYVGIAGRESVRSKSFF
                     WALKQSNKLIRVSVQSEKHRNVEGKCFVDQIFKGYETVRGKWDTVILTVTTDAYIEVIK
                     QLNKEVLQYVRCIVLISPTFGSNNLISSYINNINSNVEIISFSTYYGDTRWIHDNPSNH
                     ALTTAIKKTVYIGSTHYPSENIVVLCDIYKELGINLEVMRSPIEAETRNISLYVHPPLF
                     MNSFSLNAIFEDRDTKKFVYKIYPEGPITQYLIRDMLSQWKEVMNILRHMKVEELNLLK
                     FMIDDNYPIRSESLSRQDIENFPHLETIHQEYLLYVRYTSLLIDPYSEPDKNGRYFDFS
                     AVPLRKVFVNREGYLDIPRMPKEDYYRIKIIQGIARFLNLDCPTIDKFINTYESKIEGF
                     AQSQKEKLLSNAFNVQSFTEDLNMICNKIGKNKQQSY"
     gene            complement(11327..12142)
                     /locus_tag="HPB58_RS14250"
                     /old_locus_tag="HPB58_14205"
     CDS             complement(11327..12142)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     opine-like-metallophore: CntL"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009676653.1"
                     /locus_tag="HPB58_RS14250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HPB58_14205"
                     /product="methyltransferase domain-containing protein"
                     /protein_id="WP_243509650.1"
                     /sec_met_domain="CntL (E-value: 3e-97, bitscore: 323.4,
                     seeds: 4, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNELENFLKDLNEYLERFNDLTVSYNHTVNNCSELEALINKYSHF
                     ITNVENQGIWNQLENQENGDLDQVVNHLRKKSALCVAIMEKYRAMKLLNGETEITDYFK
                     NVESCIEKEFGSFQITPDSKVLLVGSGAFPMTPILIAKRTGAEVVGIDIDDEAIELGLK
                     VIEILGTELNIRLEKTPVEYLNFTKDATHIIFSSTVKDKYRILDQLHELTNEDVVVAMR
                     YGDQLKSLFNYPSRKVDKQKWNKAEDILRPHDVFDIELYKKAPKFITYI"
     misc_feature    complement(12150..12166)
                     /note="TFBS match to NtrC, Nitrogen regulatory protein C,
                     confidence: weak, score: 20.64"
                     /tool="antismash"
     gene            complement(12171..13004)
                     /locus_tag="HPB58_RS14255"
                     /old_locus_tag="HPB58_14210"
     CDS             complement(12171..13004)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009676614.1"
                     /locus_tag="HPB58_RS14255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HPB58_14210"
                     /product="diaminopimelate epimerase"
                     /protein_id="WP_243509652.1"
                     /transl_table=11
                     /translation="MEQEIHFVKLNPTQNMTILVETAHPAKAHKDIATKIMSYDSVYAE
                     QVGFIEEPISYKADAHLQMAGGEFCGNACMALAAFIMSKQELKHNRVKKILLEVSGTDS
                     LVECEVKRGADEYYCQVTMPIPKEIKQRTINHEGNKLDIVLIDYGEFVHIVRNVKDFNE
                     RVKEEAQSLAKSLALTLNVSLIGILLYNQDSHELEPLIYIPHLDSMVWERGCGSGTASL
                     GSYLAWKNKRETTIHVKQPGGTITAIANYQERKLISLKIEGSVGIVAQGKAFITV"
     misc_feature    13073..13093
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 27.34"
                     /tool="antismash"
     misc_feature    13615..13628
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: medium, score: 20.07"
                     /tool="antismash"
     misc_feature    complement(13809..13823)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     strong, score: 24.34"
                     /tool="antismash"
     gene            13833..15338
                     /gene="nikA"
                     /locus_tag="HPB58_RS14260"
                     /old_locus_tag="HPB58_14220"
     CDS             13833..15338
                     /GO_component="GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="nikA"
                     /gene_functions="transport (smcogs) SMCOG1068:
                     extracellular solute-binding protein family 5"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003251189.1"
                     /locus_tag="HPB58_RS14260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HPB58_14220"
                     /product="nickel ABC transporter substrate-binding protein"
                     /protein_id="WP_243509655.1"
                     /transl_table=11
                     /translation="MIFAGLLSACENSATETKNKEEDKHVHFLYNFSTNSLDPHVDSSY
                     VPLRAGITETLVRLDEKNLTIKPWLAESWEGEDGQHWTIKLREDVKFQNGKKMDANAVK
                     ASLERALKESVAVKNALKINNIQADGYSLHITTTEPFPEFVSELVNPNVSIIDVTEGDI
                     VNKPIGTGPFALESFTPGSKLELKRYNDYWDGASKLNSVTFSFNEDANARSLALKSGQV
                     DVVYRPEVETLETLKAMVGIKVESTATFRVHQMTMNMQRKSLQDVNVRRAVDALIDRQK
                     IVDTILLGHAEVAQGPFLPSLPFAPKYENSQSEKGADVAIKHLKKAGYKLKDGKMRKNG
                     EPLKFTLLTYSARADLPLIAQVFQSDAKKIGIDVEIRQIDIPEEYMASNRDWDLATYSN
                     LTAPRGDAGYYLNATYHPTGALNFSGASDPKLTAIIDKLNQTVNQEERATLSEQAANYI
                     NEHVINSFVLHPSTIVAYNEDKVKNWVTTRSEYYMITNKLDVK"
     gene            15343..16290
                     /gene="nikB"
                     /locus_tag="HPB58_RS14265"
                     /old_locus_tag="HPB58_14225"
     CDS             15343..16290
                     /GO_component="GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="nikB"
                     /gene_functions="transport (smcogs) SMCOG1118:
                     binding-protein-dependent transport systems"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017691506.1"
                     /locus_tag="HPB58_RS14265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HPB58_14225"
                     /product="nickel ABC transporter permease"
                     /protein_id="WP_243509657.1"
                     /transl_table=11
                     /translation="MIQIIVRKFLEVFIFMLFITFVSFLFIRLAPGDPVLTILNVDELS
                     VSQEQVEMLREEMGFNKPLLVQYGYWLLNFIQLDFGQSYVTGQPVIEMILTGLPATVEL
                     TIGSMIVMLAVSIPLGSLSALYRDSWIDQISRILSIIGAAIPSFWLGLIFIDLISVHFS
                     WLPTMGRDGLSSLILPSLTLGLAISSVYVRLLRSSLLDSLGQEFIRSARARGLSDKRIF
                     LVHAFRHSLPPVITVFGVSLGSLIGGVVVIEVLFAYPGIGKLVVDAIRQRDYPLIQGYI
                     LIMSAVVFIVNTCVDLSYRYLNPEMKLKETESNK"
     gene            16287..17135
                     /gene="nikC"
                     /locus_tag="HPB58_RS14270"
                     /old_locus_tag="HPB58_14230"
     CDS             16287..17135
                     /GO_component="GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="nikC"
                     /gene_functions="transport (smcogs) SMCOG1085:
                     binding-protein-dependent transport systems"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019394407.1"
                     /locus_tag="HPB58_RS14270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HPB58_14230"
                     /product="nickel transporter permease"
                     /protein_id="WP_243509659.1"
                     /transl_table=11
                     /translation="MKGLSMHLFNEKKPVWQGIIGLLSIVFIILVSIYTFYFLKHDPTL
                     TNLDERLQEVSFQHPLGTDHLGRDVLTRLLLGGIQTIGYSSLALIVALLIGIPFGLISG
                     YKRGLVDRIFMRISDGFLAFPDTIVAIVLSGLLGPGIGNLVLAIVLVKWVSYARLVRST
                     VLSESQKDYVLVAKINGLSSIKIMQKHLFPHIVGNVLVLASLDLGKIILLISSLSYIGL
                     GAQPPTPEWGAMLNDSRPYFESKPELMIYPGLAIVVVVLLTNMLGDYLRDHFDVKKEIR
                     K"
     gene            17132..17932
                     /locus_tag="HPB58_RS14275"
                     /old_locus_tag="HPB58_14235"
     CDS             17132..17932
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019394408.1"
                     /locus_tag="HPB58_RS14275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HPB58_14235"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_243509661.1"
                     /transl_table=11
                     /translation="MILSIERVSISSREKKIVNNVSLSIRKGEWYALVGQSGSGKSLLS
                     QSIGQLLHSNLQIEGKIFFKGKNLLEFAPKQIQTVRGQKISYIFQDYQGSFTPFRTISQ
                     HFEEYQKAHGIHDPKTRELNAKEELEAVGLDETLYNRYPFQLSGGQLQRVSIALALLLS
                     PDLLIADEITTALDSVSGHRILELLDKRQKEKGCTILFITHDWRHVKRYATRLAVMKEG
                     EIVESGNKHRILDHPRHEYTKQLIQAAPTLGRGLPSGLPEVENK"
     gene            17929..18690
                     /locus_tag="HPB58_RS14280"
                     /old_locus_tag="HPB58_14240"
     CDS             17929..18690
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019394409.1"
                     /locus_tag="HPB58_RS14280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HPB58_14240"
                     /product="dipeptide/oligopeptide/nickel ABC transporter
                     ATP-binding protein"
                     /protein_id="WP_243509663.1"
                     /transl_table=11
                     /translation="MNLLTVHELKKSYQGKKNILDNISFELNKGECLGLVGESGCGKST
                     LARCLLRIEPIDSGSIYFKDKAIEQLNDRQLYPYRRKIQIVLQNPSASLNPKLKIKDSL
                     IDPYAQFKNDLNLIHFSCTSKDAFIKQLLEVVELPSSLADRYPHELSGGQRQRVTIARA
                     ISIEPEFIVLDEPTASLDVISQGAILTLLTDLRKALNLSYLFISHDLAAVKEMSQRIMV
                     MKDGKLVDEFDNEHLFNEERHSYTKELISIF"
     gene            complement(18910..19197)
                     /locus_tag="HPB58_RS14285"
                     /old_locus_tag="HPB58_14245"
     CDS             complement(18910..19197)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019394410.1"
                     /locus_tag="HPB58_RS14285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HPB58_14245"
                     /product="YxcD family protein"
                     /protein_id="WP_243509665.1"
                     /transl_table=11
                     /translation="MGSLSISEQDIINALCVYISQKKQIKPQEVEVELMYDNSYGFSAE
                     AFVDGRKQVLITANLIEAIRLWLDEYLNRDPYADIKLVLDDEEGIIALIN"
     gene            complement(19185..19631)
                     /locus_tag="HPB58_RS14290"
                     /old_locus_tag="HPB58_14250"
     CDS             complement(19185..19631)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019394411.1"
                     /locus_tag="HPB58_RS14290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HPB58_14250"
                     /product="hypothetical protein"
                     /protein_id="WP_243509667.1"
                     /transl_table=11
                     /translation="MKKVLAALLTAVLVFLPVGNFAFQDHSTTVEAKSYRSGKKSFNND
                     NNSTNNNLNFQKKKEDSTTTNKSTNAKNNKGGFMSGGLMKGLMIGGLAGLLFGSLFANM
                     GILGSILGLLINVLAIVVLIAVIRKVFVFFRDKKKKKDTNPWGA"
     misc_feature    19616..19631
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: medium, score: 22.99"
                     /tool="antismash"
     gene            complement(19953..20825)
                     /locus_tag="HPB58_RS14295"
                     /old_locus_tag="HPB58_14255"
     CDS             complement(19953..20825)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1014: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019394412.1"
                     /locus_tag="HPB58_RS14295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HPB58_14255"
                     /product="LysR family transcriptional regulator"
                     /protein_id="WP_243509669.1"
                     /transl_table=11
                     /translation="MSLAKYEVFNTVVELGNLTKAAEALNLTQSAVSYSIANLEAELGF
                     PLLIRSRSGITLTSNGQRILKYIRTILHWDEKMKQEASCINGLEVGSVRIGAFTSVCIQ
                     WLPRWIKHFKQEHPFIEINILQGGYQDIEQWIANGVVDFGFVSLPTLEGFETISLKKDR
                     LVCILPYNHPLSNQEKIRIEQLENESFIIQKSGYDHDIKRIFKETGVTLKNAFEMADDQ
                     AIIAMVENELGVSVIPELVLQGLPHNVTILNIEPEQHRFISIAAPSLKEISPAAKKFIE
                     YIKPLLKSN"
     misc_feature    complement(20946..20960)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 22.75"
                     /tool="antismash"
     gene            20951..21664
                     /locus_tag="HPB58_RS14300"
                     /old_locus_tag="HPB58_14260"
     CDS             20951..21664
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019394413.1"
                     /locus_tag="HPB58_RS14300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HPB58_14260"
                     /product="DUF554 domain-containing protein"
                     /protein_id="WP_243509672.1"
                     /transl_table=11
                     /translation="MILIGALVNGIGVSIGTLVGSLLRRIPDSMKHTVMNVMGLAIIVM
                     GVQMGFKSENFLIVILSLAIGAVIGELFALEDKLTNIGGWLEKKIGSSKEGSISQGFIT
                     GTLVFVVGAMGIIGALDSGIRGNHDVLYTKTIIDTFVALILTTTLGIGVLFSAIPVILY
                     EGSIAIFATQIERFIPHALMNSFITEMTATGGIMIIAIGSNLVGITNIKVANLLPGIVV
                     TALLVSIIYGFKLHF"
     misc_feature    21964..21984
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 23.46"
                     /tool="antismash"
ORIGIN
        1 atggattgtc acaagcttta caccatctgg aaatgtggct tcaacttgta tgctctctaa
       61 catttcagga accccttcca ttaaatcttc tacactaagc acatgacgac caagttccat
      121 aatctcttca acactttttc cttcgcgagc tctttcgatg acatagcaag ttaaataggc
      181 tgttgcttca gggtaattca ataaaatccc ccgctctttc ctgtcttggg ctagctgacc
      241 agcaacaaca attaacaatt tttctttctc tacttctgtt aatctcattt cataaacctc
      301 cttaatatat acaaaatatg taaataaata tagcataaga gattataaaa agtaaacact
      361 attaagaatt tttagattat ttaatctatt ctatttcata tttttgttac acatagataa
      421 aatacagcaa aagtaaaatg aggaaaactt taaattctct tagtattatt cccagaagta
      481 ttgtttatct ccaagtagca ctgttgttag ccatgctagg ttatcaacct caaaaacggg
      541 gggcttataa taaaagctcc ttggcagttc aaacacagta tgatgtaatg aaatcattga
      601 gaagaagttc tagaatgtaa ttcgtatttt aaatagtcaa catgctctcg ttgtttttgt
      661 aatatttcat gaacgagaca taatctttcc tcatcaagtt tatttgaaac taattcttca
      721 gtcattttga ttccatataa attttgtccc ttaatagcat gttttaaaat atcttgttca
      781 ctagtagaag aatcaaatag ctggcttata aaaagctcta tttttcctat aattccttca
      841 ctacttttag gtgttccgtc tagtatttga acacgttttg aaagcaattc tgcatgatgt
      901 tctagatctt tacgaatttc aataaagtga gaaagaagtg aagaagagga atcgagattt
      961 tttataaaat tttcgtaagc atgtatcccc atgtattgac cttccactag tttattaagc
     1021 gtatctacta tctgtttctt atccattgac accattcctt tcgtttacat ctttaccagc
     1081 cccgattatc catatacaaa tgtatactgg atgtgttgta cattttttaa gagtcttata
     1141 ctagatatag ttctagaaat tagtgttgct taaaatgcaa gttaaagtta atttagaaga
     1201 tctctacaaa actaaaattc atttacccat aaaagtataa ttaatgagaa agaaaaaagc
     1261 gcaacctcgt tgttactgaa cgaatttgcg cttgtattac tttcttaata aaaaggctat
     1321 gtaaactaaa tgtatatata gttggttctt acaccccctt ttttaaaatt aaattaatta
     1381 ggttttgttc cgctttcttt tcagcttcat cagtatcaga atggaaataa ctcaataatt
     1441 aaaaatagaa caggtgaaac aagtaaagca ggaataaggt ttaaggtttt gaacttttta
     1501 atttctaaga tatttaaccc tgatgccaga atgagtattc cacctacaat actcaattca
     1561 tttaacaggc tatgattaat agcaccttct agcaagattg taatgagata aatagagcct
     1621 tgccatataa ataatacaat cgcggcaata gctattccta taccaaaggt tgaagctaga
     1681 acaatagaag tgattccatc taatattcca ttagcaagta aataggtata gtcttctttt
     1741 aaagcggctt ctatcggtcc taaaatagac aaagatccta tacagaataa taaaatggcc
     1801 gttgacaaac cttcagctag gttactttta gaaaatttgt tcaccaagtt gttaaatctt
     1861 acttctaaat tcaatttttg accaattaac cctcctatcg ctaaacttac aatgaaaagt
     1921 acaggatact tactcgaagg taaatgatgt acgacagcat caattcccaa cacagtagct
     1981 accaatccca tagcttgcat taaaatatta tgatactcat gttttattcc ctttttaaag
     2041 atactaccta taacactccc tactatgatt gtgagtgtat tgaaaattgt tccaaacatt
     2101 gtatgttctc ctttctgttt ataaaataac ttagaacgta ataaaacatt aaaccctcca
     2161 gtagctggag ggtcaagtgg gattttaatt tttagaacat agaatgtcac aaacgactat
     2221 agggcaataa tatttcggtg acatatttat ttaagtcatt cgtaacgcca taatcaatta
     2281 aggttatttc cattgaatca tcaatcagtt tgtattgatg atcccttata tattccatca
     2341 acttcttata ataaggaggc gcatccatat gggtgccttt aaagcagatt tgaagatatt
     2401 ctctaggagg aaaaacagtg gtaggagatg caacttcatc gtcctcttct actgtcataa
     2461 aaacacttga atacttatta aaagtccccg cacacaagtc agatactgaa atggaaaggc
     2521 ctattttccc tagaaaaata tttgttttaa gattgaaatt agtacgtaac tctgaaatag
     2581 gaaattcaat atcttcactt gggctatagt catgctgaag ataagctaca tgccgaccag
     2641 gtagtttcac ctcagcaatg gtatcaagtg gtgtatttat agcatgatta atctgcagta
     2701 gacgctgaga aattttcctt tcaattgttt ctaactctat ttttttctga gctatttcag
     2761 cttgttgatt ttttaacatc acaatcaggt tatcaatgtc ccggtagtta aaaaactcta
     2821 aaagctcgct tattggtaca cctagtgccc ttaagtattt aattgaatct agtctttcga
     2881 attgctgggt tgaataataa cggtaatggg tcttatcatc tgtaaactgg ggtcgtaaaa
     2941 gaccaatttc gtcataatat ctgagagtgg agattttcat atggaatagt tttgatatct
     3001 ctccaatcgt aaaaagttct ttcatccgat cttctccttt cgtaatcctt catcgtcttt
     3061 atctagaaaa ctacgactac ctggtgtgtt tatcttcccc taatattttt ctatgtaagg
     3121 atgattaaga aattggtcca tctctgcaaa cacaagctga caaattctcc atcctttaac
     3181 tagtttaagg gggagacgat ttgaattata taattcaagt gtaatttgtc tttcaaaacc
     3241 cggttctacc taaccaacac ttcaaacaag attctattca aatctctgtt taagttttct
     3301 atcatataaa gatgtaagcg cgggtaattt attactaatc ttactaaaat ccaggaaagc
     3361 tcgcctctat agagacaagc tttttatact aaaattcata ttattgtttg cttctaacct
     3421 acttcatata atgtaaaaaa tattaattaa ttagataatg aaatagggat tttattttcc
     3481 ctcaacaaat gccttacgtt tatcataggc aaaaacaatc attattccac ctaaaatagc
     3541 acaaatccta tacataaatg agtatgatga aatgtcagct atggggccca taataacccc
     3601 tcctagtgac acccctaaat ctgccatagc aataaatagg ccaattaata tattacgatt
     3661 tacttttggt aaaacaaaag tcaaatatgt agttaaggta ggatatataa gcgcttgagc
     3721 tattcccatc aagactgctc cagcatagaa aaacattgct cccccggtta tagaaaagct
     3781 tacacattgc gctgctaatg ctaggaggag cattgtaccc attacaaaag gagcatgcca
     3841 tttaccatct gaaggaattt tttttcttaa agtaaaacga gcaaacacaa cagttcctgc
     3901 ttgaatcatt agataaactc cagcgctccc attttttaat tccatagcgt agagaggcaa
     3961 gaaggtagta atagctccga atacaataga ggctactaac attaacacgc tacatttgaa
     4021 caaaaatgga ttttttatta actcaccaaa tgatttgaac atatttactc tttgatgcgt
     4081 attatttatt gtagttaaat cctctcgctt atccacttta gcactataac caaaaaaacc
     4141 tgtaaaaata gcaatcgata tcattactat tgtaaagtaa gctgtattgt ttgcttgcca
     4201 aattcctaat gctaatatag gtccgataac acctggtata tgtgagaata aagaataaag
     4261 cgagatacct tgagaacgat cctcctctgg aagggcatca ataataccaa tttgtaacgc
     4321 cattgagaaa aaagctgttg ataccccttg taagatacgt gcaattaaaa atccacctaa
     4381 tccagtgatt gtgtagagta ttaaggcaaa tccattaatg ataaggatta tacgtaatac
     4441 ttttataggc cctttttttt gtataatatg tcctgcccat ggtcgaaaaa acatagtggt
     4501 aaacaaataa gctcccataa taataccgat ggttgtattg ctagctccta atgtttctcc
     4561 ttgtaaagga ataataacat ttagtataga gtttgcacta aagtaaagaa gggttaatcc
     4621 gtataaacgt aaaaaaggcc aagacatagc tccattcaca actttttctc ctcctaattc
     4681 attttaatat cttcctaaaa ttctatcatt catgcttgga taagaatggt ataaaccata
     4741 tcagaaagtt attaatttct gcagtaattc tctggcgtat tctgattgaa catttttcaa
     4801 atttgaagtt tccactgttt cttcaatttt tccattttta aaaataataa ttctgtcaca
     4861 aatgtaagct gcagcctgaa tatcgtgagt aataaagata tagcctatgc gatagatttc
     4921 ctttaattcc tttaataatt taagtatttg aacttggaca gatacatcta aagagctaat
     4981 tgcttcatct aacaaaatac atttcggttc tgttgcgata gccctagcaa tacataccct
     5041 ctgtacttgt cctcctgata actcatgagg atatttttct ttatacgagg agtccaaccc
     5101 cacttgattc aagaggtaat caatcctact ttcaatttct attttgttac ttttcttaac
     5161 tcttatgggc tctataatag cttgttctac agtgaaaaat ggattaatag aagatttata
     5221 gtcttggaat acggcactaa tactgcctaa tctagttaat ctatcattca tacttctccc
     5281 ttcaaacaaa attcttccgg catcaggttg ttcaatacct atcaatagac gccctaatgt
     5341 tgatttgcca cttccacttt ctccaataat tcctatacat tctccactct tacattcaaa
     5401 actaatgttt tttaatattt tccgtttttc cttagagaat aacctacctg tcttatagac
     5461 cttctctaca ctttctacaa ttaacattta ttattcatcc ccattacctt tttaaaatga
     5521 ttactcagta cctgtttagt ggataataaa taatttgtgt attcatgttg tgcctgtgag
     5581 aagatggtct gaatactgcc tctctcaaca atccttccat ctttcataat taagatgtca
     5641 tctccaactt tttttacaac acccaaatca tgcgaaataa aaatcattga gcatcctatt
     5701 ttttctctta gctttataaa ttgctctaca acttcaaatt gtgagattgt atctaatgct
     5761 gtcgtaggtt catctgctat gataatatcg ggctctaaag ctattgctag tgcaatcata
     5821 agacgttgta acattcctcc tgataactga tggggatact tattcataat ctctatagga
     5881 tttttcaaca ttacgctttc catagtgctc ctcatctttc tttctatttc tttattgttc
     5941 catccaaaat gctcaaagag tgtttccttt agatgcattc caacgacaca agaagggtca
     6001 aatgcactca taccgttttg gggaatcatg caaaggtttt ttcctctttt ctttctcatt
     6061 tctttttctg taagagtatt taggttttca cctttgaaca gaatagtgcc agattgagaa
     6121 agtgaaggtc tatttaacct cattattgat ctacaagtta cagattttcc gcttccactt
     6181 tccccaacga ttgctaaaca acttccctgt ctaacactga aagaactatt atgaatgatt
     6241 accttgccgt tcttaatatc ttgtattcta agattttcga cttctaatat attcatatat
     6301 ttattttgcc acctctttct tctggatttt atgtctagga gatactaacc ttcctttaat
     6361 tctattttta gaactcatta atttaggatc taacgctact tgaagggcat cagataaaaa
     6421 gttaaaggct gatactacaa taataatggc tagaccagga gctaacatta actctggacg
     6481 tgtaaacatt acttccttag cctcacttaa catcatcccc cattcagcat ttggggcttg
     6541 tatccctaat cctaagaatg agagacctga tatttgtaaa accatggaac caacagagct
     6601 acttgagagg actatcacat ctgataaggt aacaggcacg atgtgtttat aaataatcct
     6661 catattacta atgccacaag ccttagcaaa ctttacatat tccagttctg aatattgcat
     6721 aacagaagtt ctaatgactc gagtgaacca tgcccattta agaaaaataa aggcaattag
     6781 tatatttaca agccctaccc ctaaaatacc aacaatagct aaagtcataa catagccggg
     6841 aaaagacagc ataatatcgc aaattcccat aataactcca tctactttcc cccgaaaata
     6901 accagctaaa aatcccaaga cagatccaat tacaacggat aggaataaag caactgaaac
     6961 ccatagcacg ctaggacgaa ttccgtaaat caatcgagaa agaatacatc ttcctaagtg
     7021 gtcattacct aataaatact cccaagatgg tgctgcatat cgaagcccaa tatttacttc
     7081 tttagggtta tgcggggcaa atataggtga gaatatccca ataaccattg ttagaatgat
     7141 aatgatcaat gacacagtag ctaatttgtc tttactcaag ttttttaata ttctcattta
     7201 taaatcattc cttaatttag ggtttatagt agcatttata atatcagaaa tagtgttaaa
     7261 tattacgaaa gagactgcta ataaaagaac ataagcttga ataaccggaa agtctctact
     7321 caggatagac ttaacgctta gacttcctaa tcctggccat gcaaaaacat tttcgatgac
     7381 tactgtgctt cctaatataa taggtatcgc catacaaaat actgaaatgg aaacttgtaa
     7441 tgaatttctt aaaatctgta gggtaattgt ctgctcattt aatccacatg ctcttccata
     7501 taatacgtag tcttcattta agttatttag catagagctt cttacaattc tgaaatagat
     7561 accggcataa cttattgtaa ttacgataac agggaggata taacttttgt atgaactcat
     7621 cccgcttgta ggtaacaaat ctaatttaat agagaagtac caaattatca tggatgctag
     7681 ccagtaggct ggtgctgatg ttagaaagaa agagacccct cttactgatt tatcaaatag
     7741 ccttccctct ctcatagcac atagcactcc taggaatatt gacagaacga taacaaaaac
     7801 agatgatact gatgttagct ttaatgtatt taaaaatgca ggtcctatta atgaccaaac
     7861 cgattgtcct gtaacgtatg aattcccaaa atccaactga aaacaagcta ttacccagtg
     7921 taaatattgc atgataaatg gctgatttag tcctaattct tgttttgttt ccataattag
     7981 ttcatctgtt atctttggaa caccttgagc ttgtaatacc acttcagctg gatcttgagg
     8041 agaaagatta attaaaataa aagttaggaa tgagatgact aaaagtaatg gtatagatat
     8101 taataacttt ttaataatgt aacttcccat atataatctc ctttttaaaa atagaattaa
     8161 tagctagatt aattatgtgg atagtgttca ttacaaataa aaagggagat aatctccctt
     8221 tttatttgta atttctattc aaagtgcatt ttctcaaacg gtaattcgaa ttgtgtttgt
     8281 ttaaacaaaa ttccttttaa attctttggg gcaattacag ttacactgcc attggatatt
     8341 ggaataaaga cggcttcatc atgaacaatt tttagaatat cagcgtacaa tgatttacgt
     8401 gtttcttcat tcatagatac cataacatcg tcaattttgt tatagagttc atctgctttt
     8461 tcaattcctt tagtagtgtg taaataagaa gatggggaag taaaagcaga aatagtactt
     8521 tgcggatcat atgctaatcc ccatgtctga ttgaataata aatcatagtc accagttgat
     8581 cttctatttg caattgaagt tgactcctca ccaataatct ggagctgaac acctatctct
     8641 tttaatgaac tttggataaa ttcagcctgg gtcttttggg aagaagaact gctgtcataa
     8701 taaagcttca ctgccaattt ttcaccatct cttattctaa ttccattact atcttctatt
     8761 gcccatcctg ccttttgtaa aattcccttt gctttattta aatcgtaacc cctctttttc
     8821 aaattaacat ctgcataatt aatgttatgt gaaaatagag tatgggctac agtttctgtt
     8881 ccacttagaa tctctttact aattgttttc cgatcaatag cgtaccaaat agcttcacgt
     8941 acaggttttt catgtactgg attaccagat tgactactat tagctacgat cattttagta
     9001 ttcattggtt cacttctaat taattgataa tcacccaaat caactaattg gtccatagcc
     9061 tcaacatcca gactatctgt ccccctgtca tctgtaaaag caaagttgac ttctcctttt
     9121 tgtaaggcta gaaacgttgt ttctcctgct ggaagaactt tagaagtaat ttttttaacc
     9181 ttaggcactc cattccagta gttttcattt gctttaaagg ttgcatattc atcaacttta
     9241 tgttcggtaa gcacatatgg acctgtacca tggtaacctc tgactccccc ttttgtttct
     9301 ccattaataa aatctttagg tgagataaat acataaggtc ttgtcattga taactcaact
     9361 aaggtaggat aatatgcttc tgataatact aattcaacgg tatattcatc tataacctta
     9421 caatctacta tttttgttga gagtttaatc caagaatgtt tttctgcatt agcttgtaca
     9481 gcatcaatat tctttttcac tgcttctgca ttaaatggtt ctccatcatg aaatttaaca
     9541 tcttttctta aatgaaaagt atacgttttc ccgtctttag agatattcca tgactcagca
     9601 agtaaaggtt taatcccatc ttctgtgttt tcaactaacg attcatatac tatggcctgc
     9661 gctggcattg aacctgtata taaatgagga ttcatatcat tgatatcttt agatgctgca
     9721 taaactatac ctttctcgtt acttttcgag tcagtttctg ctgttttctt acgttcagaa
     9781 gtatttaata ctacaaggca aacaactaca atacataata taaaaaaaac taaagcccct
     9841 aacttaaatc tcttgactag caatattctt tctctccctt taaatgcact tagtctgtat
     9901 ccaatatagg tttattgtgc gaatatgaac gtcataataa atagaataca tcccaaattt
     9961 cgtaatcatt acgatttaaa aatcatttta ttccattaat ctaatagctt tgctgcttat
    10021 tcttacctat tttattgcat atcatattta aatcctcggt aaaactttga acattaaagg
    10081 cattggatag aagtttttct ttttgagact gagcaaatcc ttctatcttg ctctcatacg
    10141 tattaataaa tttatctata gttggacaat ccaaatttag aaaccttgca attccttgta
    10201 taatttttat gcgatagtag tcctctttag gcatccttgg aatgtctaag tatccttctc
    10261 tattaacaaa tacttttcta agaggaacag cagaaaaatc aaaatatctt ccgttcttat
    10321 ctggttctga ataaggatca attaataagg acgtataacg tacatacaat aaatattctt
    10381 gatgaatagt ctctaaatgt ggaaagttct caatatcttg acgagataaa ctttctgatc
    10441 ttattggata attatcatct atcataaact tcagcaaatt aagttcttct actttcatat
    10501 gccttaaaat attcataact tctttccatt gagatagcat atcacggatt aaatattgag
    10561 taatagggcc ctctggatag attttatata caaatttttt cgtatctcta tcttcaaata
    10621 ttgcatttaa tgaaaaggaa ttcataaata atgggggatg gacatataaa gaaatatttc
    10681 ttgtttccgc ctcaattgga gaccgcataa cttctagatt aatgcctaac tctttatata
    10741 tatcacaaag cacaactata ttttcagacg gataatgtgt agatcctata tagactgttt
    10801 tcttaattgc tgttgttaat gcatgattag atggattatc atgtatccat cgggtatcac
    10861 cgtagtatgt agaaaaacta ataatttcta catttgaatt tatattgttt atataactac
    10921 ttattaaatt gtttgaacca aaagttgggg aaattaaaac tatacatcta acgtactgta
    10981 atacttcctt attaagctgc tttattactt caatatatgc atcagttgtt acagtcaaaa
    11041 taaccgtatc ccatttaccc ctaacagttt cataaccctt aaaaatttga tcaacgaaac
    11101 atttgccttc tacattacga tgcttttcac tttgaacact taccctaatt aatttattac
    11161 tctgctttaa agcccaaaag aaacttttcg aacgaacaga ctctcttccg gcaatcccga
    11221 catagcaatt tgattgtttc tttaaactga cagcaagctg aattgatgtt ggaccagttc
    11281 ctaatattag aattctctta aaatcactca tagaactctt cccttcttat atgtaagtga
    11341 tgaatttagg cgccttttta tacaattcaa tatcaaacac atcatgtgga cgtaatatat
    11401 cttctgcttt attccatttt tgtttatcta ctttccttga aggataatta aataaggatt
    11461 ttaactggtc cccatacctc atagccacaa ctacatcttc gtttgttaat tcatgaagtt
    11521 gatctagaat tctatattta tctttaacag ttgaactaaa aataatatgg gtagcatctt
    11581 ttgtgaaatt aagatattct acaggtgttt tttctaacct aatatttaat tctgttccta
    11641 atatttcgat cacttttaat ccaagttcta tggcttcatc atcaatatct atccctacaa
    11701 cctcagctcc tgttcgtttc gcaattaata taggagtcat cggaaaggcc cctgaaccta
    11761 ctaataacac ttttgaatca ggagtaatct ggaagctgcc aaactctttt tcaatgcatg
    11821 actctacatt tttaaaataa tccgttattt cagtctctcc atttagaagt ttcattgctc
    11881 gatatttttc cataatagct acacacagag ccgatttctt tctcagatga tttacaactt
    11941 gatctaaatc accattttct tggttctcta attgattcca tattccttgg ttctctacat
    12001 ttgttatgaa atgagaatat ttatttatta gcgcttctaa ctctgaacaa ttgttaactg
    12061 tatgattata gcttactgtt aaatcattaa atctttctag gtattcattt aaatctttta
    12121 gaaaattttc tagctcattc atttattctc gctcctatat agtgtaatag ttaaacagtt
    12181 ataaaagctt tcccttgtgc tactattcct acagatcctt caatcttaag acttattaat
    12241 tttctctcct gataattagc aattgctgta atagttccac caggttgttt tacatggatg
    12301 gtagtctctc gtttattttt ccaagctaag taagatccta aagaggctgt acctgagcca
    12361 cacccccttt cccaaaccat actatctaag tgtgggatat aaataagagg ttccaattca
    12421 tgagaatctt gattatacaa aagaatcccg atcaaactta catttaaagt taaggctagt
    12481 gattttgcca atgattgggc ctcttctttc actctttcat taaaatcctt tacgttcctt
    12541 acaatatgta caaactctcc gtaatctatt aagacaatat ccagtttatt cccttcatga
    12601 ttgatagttc tttgtttgat ttcttttgga ataggcatcg taacctgaca atagtactca
    12661 tctgctcccc tcttcacttc gcattctacg agggaatctg ttccggacac ttctaacaat
    12721 atttttttta ccctattgtg ttttagctct tgtttagaca tgataaaagc tgctagtgcc
    12781 atacaggcat taccgcaaaa ttctcccccc gccatttgta agtgtgcatc agctttataa
    12841 cttataggtt cttctataaa acctacttgt tcagcatata cactgtcata tgacataatt
    12901 tttgttgcga tatctttgtg agctttggct ggatgagcag tttcaactag aattgtcatg
    12961 ttctgagtcg gatttagttt cacaaaatgg atctcctgtt ccaatcgcac ctctccttta
    13021 ctaattaatg aactaaaaat catgtaaata gtaataatta cgatttacat ttaaggatag
    13081 cttatactat gttatttact atcgtcaata cctctttttc tattttatgt taaacactga
    13141 atataactgt aggatgtttg tgggatcccc tccctagcgg aaaatcaatt tttacagata
    13201 aggcatacac tataaacttc aaatttgtgg tgactaatct gataattaga gtccatgttc
    13261 atggggcaaa tatctaattc tgttatactt agacgttttg tgcaaataaa gaagtgagaa
    13321 tgagtagtaa gtttactctt caaacaaaaa attgtttcta ttaaaccaat gtctctaaaa
    13381 aggaagatat tacgatatac agtatccaag ttaacatttc ctgatgcttt ttgaagggat
    13441 tgtataacta acttgactga tataaactga tatcgctaat gtacggaaaa tgataaaatt
    13501 tctaatcgtt taggagtaaa cttgtagtca ttatccctga ggttaatttt atgatcttcc
    13561 aaattcaaag ttctcccctc cttcccctcg tccctattta atttcaatta ttgaagaaaa
    13621 aatgttcgct tctaaacaaa acaacaagtt aaagatataa actataaaat tattaatatt
    13681 gctaaatagg aatgattacg atagaatata aatcgtaatt atttcgtatt agggggcgta
    13741 tatttaaaat ggttagaaat agagactgtt ataggattta taatattttt cagaggtaca
    13801 ttaggaataa ttttctaaaa atagggtttt taatgatttt tgcaggttta ttgagtgcgt
    13861 gtgagaattc agcaacagaa acgaaaaata aagaagaaga taaacacgtt cattttctgt
    13921 ataatttctc tactaattct cttgatccac atgtagattc aagttatgtt ccgttaagag
    13981 caggaataac ggaaacgcta gttcgattag atgaaaaaaa tttaaccatt aaaccatggc
    14041 tagctgaaag ctgggaaggt gaagatgggc aacactggac aattaaactc cgtgaagatg
    14101 ttaaatttca aaatggtaag aaaatggatg ctaacgcagt taaagcttct ctcgaacgtg
    14161 cacttaaaga aagtgttgct gtgaaaaatg cactgaaaat taataatatt caagcagatg
    14221 gatactcctt acatattaca actactgaac catttccaga gtttgtctca gaacttgtaa
    14281 accctaatgt ttcaattatt gatgtaacag aaggagatat cgttaataaa cctataggta
    14341 cagggccttt tgcattagag agtttcactc ctggaagcaa actggaacta aaacgttaca
    14401 atgattattg ggatggagct tcaaaactta actctgtaac cttctcattt aatgaagatg
    14461 caaatgctcg ttcgcttgcc ttaaagtctg gtcaggtaga tgttgtttat cgtcccgaag
    14521 tagagactct tgaaacccta aaagcaatgg ttggcattaa agttgagtcc acagcaactt
    14581 ttcgtgtaca tcaaatgaca atgaatatgc aacgtaaaag tttacaggat gttaatgtac
    14641 gacgagctgt agatgcgtta attgaccgtc aaaaaattgt ggatactatt ctgcttgggc
    14701 atgctgaagt tgctcaaggt ccgtttcttc cgtcccttcc ctttgctcca aagtatgaaa
    14761 atagtcaaag tgagaaaggt gcagacgtag ctataaaaca tttaaagaaa gccggttata
    14821 aacttaaaga cggaaaaatg cgaaaaaatg gagaaccact taaattcacc ttactaactt
    14881 attcagcaag agccgattta ccattaattg ctcaagtttt tcaatcagat gctaaaaaaa
    14941 ttgggattga tgttgagata agacaaattg acattccaga agaatatatg gcgtcaaacc
    15001 gcgattggga cttggctact tatagtaatt tgacagctcc tcgcggagat gcaggttatt
    15061 atttgaatgc cacttaccac cccactggtg ctctaaactt tagtggtgca agtgatccga
    15121 aattgacagc tatcattgat aaactcaatc aaacagtcaa tcaagaagaa cgagctacac
    15181 tttcagagca ggcagctaat tatataaatg agcatgtaat caattcgttt gttttacatc
    15241 cttcaacgat cgttgcctac aatgaagata aagttaagaa ttgggtaact acccgtagtg
    15301 aatattacat gattacaaat aaactggatg tgaagtagat agatgattca aattattgtt
    15361 cggaagtttc ttgaagtttt tatttttatg ttgtttatta cctttgtaag ctttttattt
    15421 attaggcttg cacctgggga tcctgtgttg actattctga atgttgatga actatctgtt
    15481 agtcaagaac aggtggaaat gttgagagaa gaaatgggat tcaacaagcc tttactcgtt
    15541 caatacgggt attggcttct taattttatt caacttgatt tcggtcagtc gtatgttact
    15601 ggtcaacctg taatagagat gattttaact ggtcttcccg ctacagttga attaacaatt
    15661 gggtcaatga tcgttatgtt ggcagtatcg attccattag gttccttatc agctctgtat
    15721 cgggatagtt ggattgatca gattagtaga atactatcaa ttattggagc agctatacct
    15781 agtttttggt taggtcttat ttttattgat ttaattagtg tacattttag ttggcttcca
    15841 acaatgggaa gagatggact atcctcatta atcttaccat cgctgacact tggtttagct
    15901 atttctagtg tatatgtccg cttgcttcgt tcaagcttac ttgattctct tggtcaagaa
    15961 tttattcgtt cagcaagagc tcgtggtctg tcagataaac gaatcttttt agtccatgca
    16021 tttcgacata gtctccctcc ggttattact gtatttgggg taagtttggg aagcttaatt
    16081 ggtggtgtag tggtgattga agttctattt gcatatccgg gtataggaaa acttgtagtt
    16141 gatgcaatac gccaacgaga ttatccactc atacaaggat atattttaat catgtctgct
    16201 gtggttttta ttgtaaatac atgtgtagat ttgtcttatc gatatttgaa cccagaaatg
    16261 aaattaaaag aaacggagag taataaatga aaggtctttc tatgcatttg tttaatgaaa
    16321 aaaagcctgt ctggcaaggg ataattgggt tgctatccat tgtctttata attttagtct
    16381 ctatttatac tttttacttt ttaaagcatg atcctacatt aacaaatctt gatgaacgtc
    16441 tccaagaagt gagctttcag cacccacttg gtacagacca tcttggtaga gatgtgttga
    16501 ctaggctatt gttaggcggg attcagacaa ttgggtatag ttctttagca ctcattgttg
    16561 ctctccttat tggaatcccc tttggtctta tttcaggata taaacgtggg ctagttgatc
    16621 ggatatttat gagaatttcc gatggatttt tagccttccc tgatacaatt gttgctattg
    16681 tcttaagtgg tttgctcgga cctgggatag gtaaccttgt ccttgctatc gtgttggtta
    16741 aatgggtaag ttatgctcgt ctagtccgta gcacagtttt atcagaatcc caaaaagatt
    16801 atgtgttggt tgcgaaaatc aacggactat cttcaattaa aattatgcag aagcatttat
    16861 ttccccatat tgtaggaaac gttcttgttt tggcaagtct tgatttaggt aaaattattc
    16921 ttctcatttc ctctctctca tatatagggc taggagcaca accaccaact cctgaatggg
    16981 gagcaatgct aaacgactca cgtccctatt ttgagtcaaa acctgaatta atgatatacc
    17041 ctggtttagc cattgtagtc gtagtattac tgactaacat gctaggagac tatttacgtg
    17101 atcattttga tgtgaaaaag gagatacgaa aatgatttta tcgattgaaa gagtatccat
    17161 tagcagtaga gaaaagaaaa ttgttaataa cgtgtccctt tcgattcgca aaggagaatg
    17221 gtatgctctt gtcgggcaaa gtggaagtgg aaaaagtcta ttatcccagt caattggaca
    17281 acttcttcat tctaatttac agatagaagg aaagattttt tttaaaggaa agaacttact
    17341 tgaattcgca ccaaagcaaa ttcaaacagt acgtggtcaa aaaatttctt atatttttca
    17401 agattatcaa ggttctttta caccattccg taccatttct caacatttcg aagaatacca
    17461 gaaagcgcat gggattcatg acccaaaaac ccgcgaatta aatgcaaagg aagaacttga
    17521 agctgttggg ctagacgaaa cactatacaa tcgatatccc tttcaattaa gtggtggaca
    17581 acttcaacgt gtctcaattg cattggcact tttactatct ccagatttat taattgcaga
    17641 tgaaataaca actgcgcttg atagtgtttc tggacaccga attttagaat tacttgacaa
    17701 acggcaaaaa gaaaaaggat gtaccatatt atttattact catgattggc gacatgttaa
    17761 aagatatgca actcgcttag cagtgatgaa ggaaggggaa attgttgaat caggtaataa
    17821 acatcgtata cttgatcatc caaggcatga atataccaag caattaattc aagcagctcc
    17881 tacacttgga cgtgggctcc cttcaggttt accggaggtc gaaaacaaat gaatttactt
    17941 actgtacacg aattaaaaaa atcatatcag ggtaagaaaa acattcttga taacatctcg
    18001 tttgaactga acaagggaga atgtctaggt cttgtcggtg aaagtggctg tggtaagagc
    18061 acattagccc gctgcctact ccgaattgaa ccgattgata gcggatcaat ttattttaag
    18121 gataaggcaa ttgagcaatt gaatgatcga cagttatatc cttatcgaag aaagattcaa
    18181 attgtgctac aaaacccttc agcctcttta aatccaaaac ttaaaataaa agattcactt
    18241 attgatcctt atgctcaatt taagaatgac cttaatctta tacatttttc ctgtacttca
    18301 aaggatgctt ttataaagca gcttcttgaa gtcgttgagt taccatctag cttggcagat
    18361 cgatatcctc acgaactaag tggaggtcag cgacaacgtg taacaattgc ccgtgctatt
    18421 agtattgaac ctgaatttat tgtcttagat gagcccacag caagtcttga cgttatctct
    18481 caaggagcaa tacttacttt attaacagat cttcgtaaag cattaaatct ttcatacttg
    18541 tttatttcac atgatcttgc tgctgtcaaa gaaatgagtc aacggattat ggtcatgaaa
    18601 gatggcaaac ttgttgatga attcgataat gaacacttat ttaatgaaga acgccattca
    18661 tatacaaaag agctcatttc aattttttga ccagttgtat tgtaaaatct tattgttaat
    18721 aacaagccct attattagct cgtttatagt aacttgcatt aacagaaaag aaaaagcaca
    18781 gacttgttgt cactgaacaa gtttgtgctc attttacttt cctgaattta gataaaaata
    18841 taaattgttc ccctaactcc cctgcctctt actacagcaa aaccttttat tctaatcatc
    18901 ttccatttat caattgatta gtgcaataat accttcctca tcatctagca ctagcttgat
    18961 gtcagcgtaa ggatctctgt tcaaatattc gtcaagccat aatcgaatag cttcaattaa
    19021 gtttgcagta attaaaactt gctttcgtcc gtctacgaaa gcttccgcag aaaatccgta
    19081 gctattatca tacataagct ctacttcgac ttcttgaggc tttatttgct ttttttgaga
    19141 gatataaaca caaagagcat taataatatc ttgctcagaa atgcttaagc tccccatgga
    19201 ttagtatcct tctttttctt tttatctctg aaaaacacaa aaactttgcg aataacagca
    19261 attaaaacaa caattgctag aacattaatt agtaaaccta agatagatcc taatatcccc
    19321 atatttgcga ataggctacc aaataataat ccagcaagtc cacctatcat gagacctttc
    19381 attagaccac ccgacataaa accgcctttg ttatttttag catttgtcga tttatttgtt
    19441 gtagtagagt cttctttttt cttttggaaa tttaaattgt tattcgtact gttgttatcg
    19501 ttgttaaaac ttttctttcc tgacctgtaa gattttgctt ccactgttgt tgaatgatct
    19561 tggaaagcaa agtttccaac aggtaaaaat acaagtactg ccgtaagtaa agctgctaac
    19621 acttttttca ttttattaat tccctccgta agtttaagca tgatgctttt attagcaagt
    19681 gcaaatctat tacactaacc aggttaacat atatatttta acatttttat aggctgtaag
    19741 agtattaaaa cctaatgtta atcattaaga atttattttg aaagttcatc tattaccatt
    19801 ctctcaagtg ttttcctaaa ttataagata aaaatatgat tctttcataa cttttcttct
    19861 tttacaaaaa aatgcattcg ctcttaaaat gactagcgat aagagctgtt atgttaactt
    19921 cataaaaata ccgtatacat aattagatag atttagtttg actttaacaa cggttttata
    19981 tattcaataa actttttggc agcaggagaa atttctttta gggatggtgc agcaatgctg
    20041 ataaatcgat gttgttctgg ctctatatta agaatggtaa cgttatgcgg cagaccttgc
    20101 agaactaatt caggaatgac gctcacacct agttcatttt ccaccatggc tataatagct
    20161 tggtcatcag ccatctcgaa tgcgttttta agagtaacac cggtctcttt aaatatacgt
    20221 tttatatcat ggtcataacc agacttttgt atgataaagg attcattctc taattgttca
    20281 atacggattt tctcttgatt actcagagga tgattgtatg gaaggataca aaccagtctg
    20341 tcttttttta gagatatcgt ttcaaaacct tctaacgtag gtaagctaac aaaaccaaaa
    20401 tctacaactc cattagcaat ccattgttca atatcttgat aacctccctg taaaatatta
    20461 atttctataa agggatgctc ttgtttaaaa tgctttatcc atctaggaag ccattgaata
    20521 caaacacttg taaaggctcc tatccggact gaaccaactt caagtccatt gatacaggag
    20581 gcttcttgct tcattttttc atcccaatgt agaattgttc gtatatattt taagatcctc
    20641 tgcccattgc ttgttaaagt aataccagac cgacttctaa taagtaaagg aaagccaagt
    20701 tcagcttcaa gatttgcgat agaataacta acagcagact gagttaaatt taaagcttcc
    20761 gcagctttgg ttagattacc cagctcaaca acagtgttaa aaacttcata tttggcaaga
    20821 ctcattttta tcattcctat ccataaattt tattcatgtt aaatattgaa aactttgatt
    20881 ttatttatat gtagtttacc tctataattt gagaaaatac aactgtatag aaacggacag
    20941 gtgaaagaaa atgattttaa taggggcttt agtaaatgga ataggtgtaa gtatcggtac
    21001 tttagtaggg agtctacttc gtcgtatacc agatagcatg aagcacactg tcatgaatgt
    21061 aatgggttta gccattatcg ttatgggagt acaaatggga tttaaaagtg agaatttcct
    21121 tatcgtgatt ttaagtcttg ctataggggc agtaattggg gagttatttg ccttggaaga
    21181 taaactaact aacataggag gttggttaga gaaaaaaatc ggctcaagca aagaaggaag
    21241 tatatctcaa ggatttataa caggtacttt agtattcgtc gttggagcaa tgggtatcat
    21301 tggtgcttta gatagtggaa ttagaggtaa tcatgatgtg ttatatacaa aaacgattat
    21361 agatacattt gtagcattaa tattgactac aaccctaggg attggggttt tattttctgc
    21421 aattccggtc attttatatg aaggttcaat tgctattttt gcaactcaaa ttgagcgttt
    21481 tattcctcat gctttaatga atagttttat tacagaaatg accgctactg gtggaattat
    21541 gattattgcc attggttcaa atttagtagg gattaccaac atcaaagtgg ctaatcttct
    21601 tcctggaatc gtagtaactg cactgcttgt ttcaattatt tacggtttta aattacattt
    21661 ctaaaaatga atatacgcca caaaaccttt ccttttcagt aatgtaattc ttgctactga
    21721 agaggaaagg tttctcactt tattcctatt tattcagaag tacaacaact attttaatag
    21781 aatataccct acactattga tgaaaaaata gactgaacgg tttttaccgt gcagtctata
    21841 aataatgttc ttactttaga aaattaatca gttttttagg acgatcagac tttttattat
    21901 acttatttgt ccttttttaa cttcctctcc atcaattgtg ccatttgttg agaatttaaa
    21961 gccttttctt catagagtct attaacgatg ttctgatagt ccccttcact acgattttga
    22021 cttagtttat tggcagcttg aatttcttgt actttaatct taaatccaac aatccctttt
    22081 agttcttttt ctaaaacttc aggagaaagc ttatcccata aaacaggatt cttacgatgt
    22141 tt
//
