LOCUS       NZ_JAICDG010000005     10867 bp    DNA     linear   CON 29-AUG-2024
DEFINITION  Actinomadura terrae strain OS3-83 contig5, whole genome shotgun
            sequence.
ACCESSION   NZ_JAICDG010000005
VERSION     NZ_JAICDG010000005.1
KEYWORDS    WGS; RefSeq.
SOURCE      Actinomadura terrae
  ORGANISM  Actinomadura terrae
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Thermomonosporaceae; Actinomadura.
REFERENCE   1  (bases 1 to 658968)
  AUTHORS   Lee,S.D., Yang,H.L., Kim,I.S., Choe,H. and Kim,J.S.
  TITLE     Actinomadura terrae sp. nov. and Actinomadura litoris OS3-89,
            isolated from rhizosphere soil of cactus
  JOURNAL   Int J Syst Evol Microbiol 73 (1) (2023)
   PUBMED   36748505
REFERENCE   2  (bases 1 to 658968)
  AUTHORS   Lee,S.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-FEB-2022) Institute of Jeju Microbial Resources, BioPS
            Co., Ltd., Sancheondan Dongil 16, Jeju 63243, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 141x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022751215.1-RS_2024_08_29
            Annotation Date                   :: 08/29/2024 06:12:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,085
            CDSs (total)                      :: 8,002
            Genes (coding)                    :: 7,936
            CDSs (with protein)               :: 7,936
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 56 of 66
            Pseudo Genes (internal stop)      :: 3 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 21:31:58
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 442281
            Orig. end                         :: 453148
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAICDG010000005.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10867
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="3"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10867
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10867
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5867](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5867)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(53..66)
                     /note="TFBS match to CatR, H2O2-responsive repressor,
                     confidence: medium, score: 18.86"
                     /tool="antismash"
     misc_feature    complement(1015..1029)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 15.53"
                     /tool="antismash"
     gene            complement(1061..2668)
                     /locus_tag="K2F47_RS31035"
     CDS             complement(1061..2668)
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /GO_function="GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1138: FAD linked oxidase domain protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF013714.5"
                     /locus_tag="K2F47_RS31035"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="FAD-binding oxidoreductase"
                     /protein_id="WP_242909443.1"
                     /transl_table=11
                     /translation="MTEHTRRNVLWGGLAATAAGIAAPALGTAPAEAAAPFTSPPPPAS
                     EVGRGDPRYPSLAGRGYNRRFVGDPEHVWVVGTTEDVVRAVQKAVDTGKRVTVRSGGHG
                     FEGFVGDPAVQVVVDTSAMTGVYYDASRRAFAVEAGALLGDVYRRLYLGWGVTIPAGWC
                     PTVGAGGHVPGGGFGPLSRMLGLAVDHLHGVEVVVVGRDGRARGVVATADASDPNRDLW
                     WAHTGGGGGTFGIVTRYWFRTPGARGDDPSRLLPAPPSTALTFSVDWDWKKIDKAGFER
                     LVRNHGTWVERNSAPGSPATALYSELQLSRAAFGTIGMIGQVAAGSGLPAAERLLDDHI
                     AAINEGVPVTPVRTVRGRDWLAAALAGSPGDPGPLYRLKVKSAYLRRRLTDRQIDVLHR
                     HLTRTDYDHPGAFLSLYTFGGQVNTARPDATAAPHRDTIMKVFLINGWEDVADDARHIA
                     FTREVYRDLFADTGGVPAADDGAFINYPDTDLADPALNNGAPWHVLYFRDNYPRLQQIK
                     AKWDPHDVFRHALSVRAT"
     misc_feature    2668..2682
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 15.56"
                     /tool="antismash"
     misc_feature    2681..2699
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 20.59"
                     /tool="antismash"
     gene            complement(2769..4211)
                     /locus_tag="K2F47_RS31040"
     CDS             complement(2769..4211)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024306.5"
                     /locus_tag="K2F47_RS31040"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ferritin-like protein"
                     /protein_id="WP_242909445.1"
                     /transl_table=11
                     /translation="MTTATTRTKSGKTASKTQSRTQTKTKAKARATAAKTRKDARKAGD
                     RLVRTRPIRQLDPAAGIKTVGDLADHLYVAAQVELSTIPMYLFAAYSIRTRGHSQWAAP
                     RGVLRSLIGISIEEMLHLSLVRNLMVAIGYGDDISFYDEDFIPSYPSTMLNRYNPEDPD
                     GPEIVLTLERLSRDQVGTFRRVEMPDDIDLGATAFARHPEDIGQYRSLGEFYRAIEQGF
                     IDLEDEIEWAVDDVRKQYKRGFWNEFGNGKPIRVHNLATARQALRIIIEQGEGTITDHK
                     TIEVRPGVEDYTHYEKFVRIQDGIEGIGAVDGGRDHEIGIDDPEATYPVVADPRVEDFD
                     GQPGVQSLMTLFNAAYCYTLCLLDETYAHSTDDVKTRKFPGTGREEMFSHRYGLERNDV
                     AAMQGILYPLAQALVTTPILDGGHKGEHAAPSFEFYDFDADEFGRTKKQQLVDLCADAI
                     EHFPILGGPDGVERQIGLLMDI"
     gene            complement(4208..5008)
                     /locus_tag="K2F47_RS31045"
     CDS             complement(4208..5008)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF021364.5"
                     /locus_tag="K2F47_RS31045"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DNA-binding domain-containing protein"
                     /protein_id="WP_242909446.1"
                     /transl_table=11
                     /translation="MPEPAAELAGIQRWMQAAILGDDTADGVEELLTTSSALTARQRLG
                     IYWRGYRLRLLENMRGLHPGLTRLLGEDMFDRFALDYLDARPSQAYTLTRLDEEFADHL
                     AATRPDDAAHRERWPDLIIDMARLERIITEVVEGPGTEDGPVLHPDDLPPTPDLRLEPA
                     PCLRLPVFAFPVNEYLAAVRRGEDPEPPAPRPVALAVSRRDYRVVMRELDPAAHRALRA
                     LAGGATVGEALAGPAPDALRRWAALGYFTRAYRVPEHPKERATR"
     gene            complement(5001..5867)
                     /locus_tag="K2F47_RS31050"
     CDS             complement(5001..5867)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017619267.1"
                     /locus_tag="K2F47_RS31050"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_242909448.1"
                     /sec_met_domain="DUF692 (E-value: 5.9e-96, bitscore: 316.0,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTWADAATNTLGFGIGLRGPHMDHVRRHRPDVGFFEVISENFMDC
                     AGGRRRALEAIAEHYPIALHGVSLSIGGTDPLDLGYLARLRDLAAAVRAPLVSDHVCWT
                     GVMGRNTHDLLPLPFTEAALAHVVQRVRIAQDVLERRLVLENPSTYVGFTASTMPEQEF
                     MTRLAEDAGCGLLLDVNNVYVSATNHDFDPVDYLDALPLERVEQIHLAGHADHGTHLID
                     THDRPVTDAVWDLYRLVTERAGPIPTLLEWDDRLPEFPVLEAELDKARLAAAGRDPVSA
                     LQGAPDA"
     gene            complement(5864..6538)
                     /locus_tag="K2F47_RS31055"
     CDS             complement(5864..6538)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="K2F47_RS31055"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_242909450.1"
                     /transl_table=11
                     /translation="MAKVRREHNGHDRAAGPEVLSSQDYAVEQHYDASGRSFGGASCGA
                     PAEGLEQHVCMGLNACNAFDIDGGAVMAGTGNCATVRHVCHGEGQCRGQGGCGYAGSDE
                     QQWRPGEQSCRFNGSCASPLNVSRVFSAGPMKGRSVWMQARRLMEDRMYRSGLAFGPSP
                     GDGIPDDLLPEYDVDDGQDLVGPVGRRGAASGDGARKRAAGDGARRKSTAKTRRAAPRK
                     AR"
     misc_feature    6547..6561
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 14.53"
                     /tool="antismash"
     misc_feature    6556..6570
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 18.44"
                     /tool="antismash"
     misc_feature    6612..6625
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 13.67"
                     /tool="antismash"
     misc_feature    complement(6649..6663)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 16.0"
                     /tool="antismash"
     misc_feature    complement(6671..6687)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 17.5"
                     /tool="antismash"
     misc_feature    6674..6687
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.21"
                     /tool="antismash"
     gene            6780..7445
                     /locus_tag="K2F47_RS31060"
     CDS             6780..7445
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="K2F47_RS31060"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="S1 family peptidase"
                     /protein_id="WP_242909452.1"
                     /transl_table=11
                     /translation="MRARISVIFACVATALSLLVPGTAAGATTAPPPPEGGRAIFGSNG
                     ARCVIGFNVVRNGSYYFLTAGGCAQGGLHIYADEARTVPLGTVAQVMGGGFKVALAQYV
                     DPKKQQPGTVHTYPGSLEITSAGMPSVGQRVCRSGPVSHVVCGSVTAVNQTINLPDGVI
                     NGVSRTSICASELPGAPYFTDGRAVGMEIGTSGGCVGGTSSYFQPISPVLSAFGVAVY"
     gene            complement(7449..8837)
                     /gene="murF"
                     /locus_tag="K2F47_RS31065"
     CDS             complement(7449..8837)
                     /EC_number="6.3.2.10"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA]"
                     /GO_process="GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene="murF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291553.1"
                     /locus_tag="K2F47_RS31065"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="WP_242909454.1"
                     /transl_table=11
                     /translation="MIPLTLHEIALAVGGTVHGAPAPGPTVTGPVVADPRDATAGALFV
                     ALAGRRGDGHDLAERAYAFGACAVLGTRAVDGPCVVVDEVVSALTDLAMFVRDMLDLVV
                     IGVTGSVGKTTTKDLLAQILEREAPTVASTGSRDDEVGLPLTVLRADRWTRYLVLEMGA
                     ARPGDITHLAWVARPQFGLVLNVGPAHLLTLGGIVGVARAKTELVRALPPAGEGGLAFL
                     NADDAAVASMEGATSAGVVTYGTDAGAAVRAVGVTVDGAGHASFILHTPSGAAVVRLGL
                     PGEHQVGNALAAASVADVLGVTTRRIAARLSSAAPRSPNRFQLVERDDGVTVIDDACHA
                     NPVSMRAALRTLATMSAGRRTIAVLGEMTGQAGETAREHAAVGRLAAELGVAILVVVGS
                     GEGPEALSEAARRAGVVTHALPRRRDVVHLLRVLLRPSDLVLVKASDEVGLSAVATALR
                     DPRP"
     misc_feature    8861..8875
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: medium, score: 26.5"
                     /tool="antismash"
     gene            complement(9130..10320)
                     /locus_tag="K2F47_RS31070"
     CDS             complement(9130..10320)
                     /EC_number="2.4.-.-"
                     /GO_function="GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /GO_process="GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glyco_transf_28"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glyco_tran_28_C"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017577442.1"
                     /locus_tag="K2F47_RS31070"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="glycosyltransferase"
                     /protein_id="WP_242909456.1"
                     /sec_met_domain="Glyco_transf_28 (E-value: 1.3e-18,
                     bitscore: 62.5, seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="Glyco_tran_28_C (E-value: 6.5e-22,
                     bitscore: 73.4, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTHVVVPQALIDRRDPVRLVVSGGGSGGHTFPAVAVVRAARTAFR
                     AHGLELQPFWVGGERGVEAKVAAEEGVPFTAIPTGGTFRGSNRLSRLSSAVGRVPVGVR
                     EAVNIVRGIRPHAVLCTGGYASVPVGLAARLCHRPLLVHEQAVRVELANRITMRGAARV
                     ALSAEPTMDLLPERLRGRAVVTGNPLRPGLADGDARAAPARLGWDGWTPSLPTVYVTGG
                     SRGARHINELVTAALPELLRYANVFHQAGAEPAGPVPAAVERLPPDLRPRYLARGFVGE
                     ELADLLALADVVVSGGGCGTVGELTALGKPSVLVLPARPAGDERRRNAEHLAAHGAARV
                     LVGEEAAPARLVGELALLLTDPLRRSEMSAMARRLGRPGAAAALTNEVLALCAAAS"
ORIGIN
        1 cgtccagacc gggcactgga cgccgctggc gatcgtcgag cagttcggca cgttggacgc
       61 gctccacccg ggccgcgtcg acctcggcat cggccgctcc gggcagaagc gccaggaggc
      121 cgcgagccgg tcgggcgcgc cgaagcgtcc cgcgcgcgag gccacgttcg tggacggcct
      181 gctcctcccc accccctacg acaccacgcg ggccgcgtcg tcgccgaggc tgctgctcca
      241 ggactcgctg ctgcgccagg ccggggcgcg gccaccgcgc ttcaccgacg ccatcgacga
      301 gatcatcgcg ctcctggacg ggacgctcag ccgcgacggc gtcgacgcgc acgccacccc
      361 cggcgagggc gcggcgctcg cgctgtggat cctcggcagc agcggcggcg agagcgcccg
      421 cgtcgcgggc gagcgggggc tgccgttcgc ggcgaactac cacgtcgccc cggcgagcgt
      481 gctggaggcc gtggacgcct accgcgcggc gttccgcccg tccgcgacgc tggccgagcc
      541 gtacgtcctc gtctccgccg acgtggtcgc ggcgcccacc gaggcggagg cgcgcgagct
      601 cgccgccccc tacgcgctgt gggtgcacag catccgggcg ggcggcggcg cgatcccgtt
      661 cccgacgccc gcgcaggccg ccgcacacga gtggcgggcc gaggacctcg acctcgtcgc
      721 cgaccgcgtc gagacccagt tcgtcgggac gccccgcacg gtcgtcgcgc agctggagac
      781 gctgcgccgc gtcaccggcg cggacgagct gctgctcacc accatcaccc acgaccacgc
      841 cgaccgcgtc cgctcgttcg agctgatcgc cgaggcgtgg gcggcctagg gcgggtgccg
      901 tgcaccggcc tcagcagtac acccgcacca tcgtggccag gttgttgggg tccagcctcc
      961 gctgcgtcca cggcgccgtg tagtcccacg ttacgccgct gcgcgcaagg cgcaatcgac
     1021 gcaccacata ggacactttt cgagcctctg cggtacgcgc tcacgtggcg cggacggaca
     1081 gcgcgtggcg gaagacgtcg tgcggatccc atttcgcctt gatctgctgg agccgcgggt
     1141 aattgtcccg gaagtacagg acgtgccacg gcgcgccgtt gttcagggcc gggtccgcga
     1201 ggtcggtgtc ggggtagttg atgaacgcgc cgtcgtcggc ggcgggcacg ccgccggtgt
     1261 ccgcgaagag gtcgcggtag acctcgcgcg tgaacgcgat gtgccgggcg tcgtcggcga
     1321 cgtcctccca cccgttgatg aggaacacct tcatgatcgt gtcgcggtgc ggcgccgccg
     1381 tcgcgtccgg gcgggcggtg ttcacctgcc cgccgaaggt gtagaggctg aggaacgcgc
     1441 ccgggtggtc gtagtcggtc cgggtcaggt ggcggtgcag gacgtcgatc tgccggtcgg
     1501 tcaggcggcg gcgcaggtag gccgacttca ccttcagccg gtagagcggc ccggggtcgc
     1561 ccggggaccc ggccagcgcg gcggcgagcc agtcgcgccc gcgcaccgtc cggaccggcg
     1621 tgaccggcac gccctcgttg atcgcggcga tgtggtcgtc gagcagccgc tcggcggcgg
     1681 gcagccccga cccggcggcg acctggccga tcatgccgat cgtcccgaac gccgcacggc
     1741 tcagctggag ttcgctgtag agggcggtcg ccggagagcc cggggcgctg ttgcgctcca
     1801 cccacgtgcc gtggttgcgg acgagccgct cgaaccccgc tttgtcgatc ttcttccagt
     1861 cccagtcgac ggagaaggtc agcgccgtcg acggcggcgc gggcagcagc cgggacgggt
     1921 cgtcgccgcg cgcgcccggc gtgcggaacc agtagcgggt gacgatgccg aacgtgccgc
     1981 ccccgccgcc ggtgtgcgcc caccacaggt cccggttcgg gtcggacgcg tcggccgtcg
     2041 cgacgacgcc gcgggcccgc ccgtcccggc ccacgaccac cacctcgacg ccgtggaggt
     2101 ggtcgacggc caggcccagc atgcgcgaca ggggtccgaa gccgccgccc ggcacgtgcc
     2161 cgcccgcgcc gaccgtcggg caccacccgg cgggaatcgt gacgccccac cccaggtaga
     2221 gcctgcggta cacgtcgccg agcagggcgc ccgcctccac ggcgaaggcg cggcgggagg
     2281 cgtcgtagta gacgccggtc atggcggagg tgtccacgac gacctggacg gcgggatcgc
     2341 cgacgaaccc ctcgaaaccg tggccaccgc tgcggaccgt cacccgcttc ccggtgtcga
     2401 cggccttctg caccgcccgg acgacgtcct cggtcgtccc gaccacccag acgtgctcgg
     2461 ggtcgccgac gaaccgccgg ttgtagccgc gcccggcgag ggacgggtag cgcgggtcac
     2521 cgcgtccgac ctcggacgcc ggaggcggcg gtgacgtgaa gggcgcggcg gcctccgccg
     2581 gggcggtccc cagggcgggc gccgcgattc ccgccgccgt cgcggccagc ccgccccaca
     2641 ggacgttcct cctggtgtgc tcggtcattc aggctcctcg atcgcgaatg accaacaacg
     2701 tagggagggc gcggaacgcg gtcacggggc ctggcaccac ctcgacggtg gactcaggcc
     2761 cccggatgtc agatgtccat gagcagcccg atctgccgtt cgacgccgtc cgggccgccg
     2821 agaatcggga agtgctcgat ggcgtccgcg cacaggtcga cgagctgctg cttcttggtg
     2881 cgcccgaact cgtccgcgtc gaagtcgtag aactcgaacg agggcgcggc gtgctcgccc
     2941 ttgtgcccgc cgtccaggat cggcgtcgtg accagcgcct gcgcgagggg gtagaggatg
     3001 ccctgcatcg cggcgacgtc gttgcgttcg agcccgtacc ggtggctgaa catctcctcg
     3061 cggccggtgc ccgggaactt cctggtcttc acgtcgtcgg tggaatgcgc gtaggtctcg
     3121 tccagcaggc acagcgtgta gcagtaggcg gcgttgaaca gcgtcatcag gctctggacg
     3181 cccggctggc cgtcgaagtc ctcgacgcgc gggtcggcga cgaccgggta ggtcgcctcc
     3241 gggtcgtcga tgccgatctc gtggtcgcgt ccgccgtcga ccgcgccgat gccctcgatg
     3301 ccgtcctgga tgcggacgaa cttctcgtag tgggtgtagt cctcgacgcc gggcctgacc
     3361 tcgatcgtct tgtggtcggt gatcgtgccc tcgccctgct cgatgatgat ccgcagcgcc
     3421 tgccgggcgg tggcgaggtt gtggacgcgg atcggcttgc cgttgccgaa ctcgttccag
     3481 aacccgcgct tgtactgctt gcgcacgtcg tcgaccgccc actcgatctc gtcctccaga
     3541 tcgatgaagc cctgttcgat cgcgcggtag aactcgccga ggctccggta ctggccgatg
     3601 tcctccgggt gccgcgcgaa cgcggtcgcg ccgaggtcga tgtcgtcggg catctcgacc
     3661 cggcggaacg tgccgacctg gtccctggac aggcgctcca gggtgaggac gatctccggg
     3721 ccgtccgggt cctcggggtt gtagcggttc agcatcgtgc tcgggtagga cgggatgaag
     3781 tcctcgtcgt agaaggagat gtcgtcgccg tacccgatgg cgaccatcag gttgcgcacc
     3841 agcgacaggt gcagcatctc ctcgatcgag atcccgatca gcgagcgcag cacgccgcgc
     3901 ggcgcggccc actgcgagtg cccgcgcgtc ctgatcgagt acgcggcgaa caggtacatc
     3961 gggatcgtgg acagctccac ctgcgcggcg acgtacaggt ggtcggccag gtcgcccacg
     4021 gtcttgatgc cggccgccgg gtcgagctgc cggatcggcc gcgtccgcac cagccggtcg
     4081 cccgccttcc gcgcgtcctt cctcgtcttg gccgccgtgg cccgtgcctt cgccttcgtc
     4141 ttcgtctgcg tcctggactg tgtcttggag gcggtcttgc cggacttcgt cctggtcgtg
     4201 gcggtggtca tcgtgtcgcc ctctctttcg ggtgttccgg tacgcggtac gcgcgggtga
     4261 agtagccgag tgcggcccag cggcggagcg cgtccggggc cgggcccgcg agggcttcgc
     4321 ccaccgtcgc gcccccggcc agcgcccgca gcgcccggtg cgcggcgggg tcgagttcgc
     4381 gcatcacgac ccggtagtcg cggcggctga ccgccagcgc caccggacgc ggcgcgggcg
     4441 gctcggggtc ctcgccgcgc cggacggccg ccaggtactc gttgacgggg aaggcgaaga
     4501 ccggcagccg caggcacggc gccggctcca ggcgcaggtc gggggtcggc gggaggtcgt
     4561 ccgggtgcag gacggggccg tcctcggtgc cggggccctc gacgacctcc gtgatgatcc
     4621 gctccagccg ggccatgtcg atgatgaggt cgggccagcg ctcgcggtgc gccgcgtcgt
     4681 ccggccgggt cgccgcgagg tggtcggcga actcctcgtc caggcgggtg agcgtgtacg
     4741 cctgggacgg ccgcgcgtcc aggtagtcga gggcgaaccg gtcgaacatg tcctcgccga
     4801 gcagccgggt cagcccgggg tgcaggccgc gcatgttctc cagcagccgg agccggtagc
     4861 cgcgccagta gatcccgagc cgctgccgcg cggtgagcgc ggaggatgtc gtgagcagtt
     4921 cctcgacgcc gtcggccgtg tcgtcgccga gaatggcggc ctgcatccac cgctggattc
     4981 cggccagttc cgccgccggt tcaggcatcc ggggcaccct gaagggccga gaccgggtcg
     5041 cgtccggcgg cggcgagcct cgccttgtcg agttcggcct ccaggacggg gaattccggg
     5101 agccggtcgt cccattcgag cagcgtggga atcggcccgg cgcgttcggt gacgagccgg
     5161 tagaggtccc agacggcgtc ggtgaccgga cggtcgtggg tgtcgatgag gtgggtcccg
     5221 tgatcggcgt gcccggccag atggatctgc tcgacgcgct ccagcggcag cgcgtcgaga
     5281 tagtcgaccg ggtcgaagtc gtggttggtc gccgagacgt agacgttgtt gacgtccagc
     5341 agcagcccgc agccggcgtc ctcggccagc cgggtcatga actcctgctc gggcatcgtc
     5401 gaggcggtga acccgacata ggtgctcggg ttctccagca ccagcctgcg ttccaggacg
     5461 tcctgcgcga tccggacgcg ttgcaccacg tgcgccagcg ccgcctcggt gaaggggagc
     5521 gggagcaggt cgtgggtgtt gcggcccatg acccccgtcc agcagacgtg gtcggagacg
     5581 agcggcgccc gcaccgccgc ggccaggtcg cgcagccgcg ccaggtagcc gaggtcgagc
     5641 gggtcggtgc cgccgatcga cagcgagacc ccgtgcagcg cgatcgggta gtgctcggcg
     5701 atcgcctcca gggcgcggcg gcggccgccc gcgcagtcca tgaagttctc cgagatcacc
     5761 tcgaagaagc cgacgtccgg ccggtgccgc cggacgtggt ccatgtgcgg cccgcgcagc
     5821 ccgatcccga accccaacgt gttcgtggca gcgtcggccc aggtcaccgg gccttcctcg
     5881 gcgcggccct ccgggtcttg gcggtgctct tgcggcgcgc gccgtcgccc gccgcgcgct
     5941 tgcgcgcccc gtccccggac gcggcgccgc gccgccccac cgggcccacc aggtcctggc
     6001 cgtcgtcgac gtcgtactcg ggcagcaggt cgtcgggaat cccgtcgccc ggggacgggc
     6061 cgaaggcgag ccccgaccgg tacatgcggt cctccatgag ccgccgggcc tgcatccaca
     6121 cgctccggcc cttcatcggc ccggcgctga acacgcggct gacgttgagc gggctcgcgc
     6181 agctcccgtt gaaccggcac gactgctccc cgggccgcca ctgctgctcg tcgcttccgg
     6241 cgtagccgca gccgccctgc ccgcggcact gcccctcgcc gtggcagaca tggcggacgg
     6301 tcgcgcagtt cccggttccc gccatcacgg ccccgccgtc gatgtcgaag gcgttgcagg
     6361 cgttcaggcc catgcacacg tgctgttcca gtccctcggc gggcgcgccg caactcgcgc
     6421 ccccgaaact gcgcccgctg gcgtcgtagt gctgctcgac cgcgtagtcc tgcgacgaca
     6481 gcacctccgg gcccgccgcc cggtcgtgcc cgttgtgctc gcgcctcacc tttgccatgc
     6541 cgtccccaaa ttcagcgaat tgccggtcca cctgcgtctt cacccgggac gaagatcagt
     6601 aagcgcgcgg ctcgacttat tacgacacgc ggaatcgaca tgcgccgccg ttgaccgaaa
     6661 attgacgatc acctgatttt gatacaaggc ggcagccaac cttggcagtt cctccccttg
     6721 ccgggggccg cgccttttcc gcactattcc ggaagaacag ccccccggta ggaggccccg
     6781 tgagagcccg catttctgtg atcttcgcgt gtgtcgcgac cgccctgagc ctgctcgtcc
     6841 ccggcacggc cgccggcgcg accaccgccc cgccgccgcc ggagggcggg cgggcgatct
     6901 tcgggagcaa cggcgcgcgc tgcgtgatcg ggttcaacgt ggtcaggaac ggcagctact
     6961 acttcctcac cgcgggcggc tgcgcgcagg gcggcctgca catctacgcc gacgaggccc
     7021 ggaccgtccc gctcggcacg gtcgcccagg tcatgggcgg cggcttcaag gtcgcgctcg
     7081 cccagtacgt ggacccgaag aagcagcagc ccggcaccgt ccacacctat cccggctcac
     7141 tggagatcac gtcggcgggg atgccgtccg tcggccagcg ggtctgccgc agcggcccgg
     7201 tgagccacgt ggtctgcggg tccgtcacgg cggtcaacca gaccatcaac ctcccggacg
     7261 gagtgatcaa cggcgtctcg cggacgagca tctgcgcgag cgagctcccg ggcgcgccct
     7321 acttcaccga cggcagagcg gtcgggatgg agatcgggac ctccggtggc tgcgtcggcg
     7381 gcacctcgtc ctacttccag ccgatcagcc cggtcctcag cgccttcgga gtcgccgtct
     7441 actgacgatc aggggcgcgg gtcccgcagc gccgtcgcga ccgcggacag gcccacctcg
     7501 tccgacgcct tcaccagcac gaggtcggac ggccgcagca gcacgcgcag caggtgcacc
     7561 acgtcccggc ggcgcggcag cgcgtgggtg accacgcccg cccggcgggc ggcctcggac
     7621 agggcctcgg gcccctcccc cgacccgacc acgacgagga tcgcgacgcc cagttcggcg
     7681 gcgagcctgc cgacggcggc gtgctcccgt gccgtctccc cggcctgccc ggtcatctcg
     7741 ccgaggacgg cgatcgtccg gcgtccggcc gacatcgtcg ccagcgtgcg cagcgcggcg
     7801 cgcatggaga cggggttggc gtggcaggcg tcgtcgatga ccgtgacgcc gtcgtcccgc
     7861 tcgaccagtt ggaaccggtt cggcgagcgc ggcgcggcgc tgctcagccg cgccgcgatc
     7921 cgccgggtcg tgacgccgag gacgtcggcg acgctcgcgg cggccagcgc gttgccgacc
     7981 tggtgctcgc cgggcagccc cagccgcacg accgccgcgc cggacggcgt gtgcaggatg
     8041 aacgacgcgt gcccggcgcc gtcgaccgtg acgccgaccg cccggacggc cgcgccggcg
     8101 tccgtcccgt aggtgacgac gcccgccgac gtcgcgccct ccatggacgc cacggccgcg
     8161 tcgtcggcgt tgaggaacgc gaggccgccc tcccccgccg ggggcagcgc ccgcaccagc
     8221 tcggtcttcg cccgcgcgac gccgacgatc ccgccgagcg tgagcaggtg cgcgggcccg
     8281 acgttcagca ccagcccgaa ctgggggcgc gccacccacg cgagatgggt gatgtcgccc
     8341 ggacgcgccg cccccatctc caggacgagg tagcgcgtcc accggtcggc gcgcagcacc
     8401 gtcagcggca gcccgacctc gtcgtcccgc gagccggtgc tggccaccgt cggcgcctcg
     8461 cgttcgagga tctgggcgag caggtccttg gtcgtggtct tgccgaccga ccccgtgacg
     8521 ccgatgacga ccaggtccag catgtcccgg acgaacatgg cgaggtcggt cagcgcggag
     8581 acgacctcgt cgacgacgac gcacggcccg tcgacggcgc gggtgccgag caccgcgcac
     8641 gcgccgaagg cgtaggcgcg ctcggcgagg tcgtgcccgt ctccccgccg cccggcgagg
     8701 gccacgaaca gcgcgccggc ggtggcgtcc cgcgggtcgg cgacgacggg cccggtgacg
     8761 gtgggcccgg gcgccggggc gccgtgcacc gttccgccga ccgccagcgc gatctcgtgc
     8821 agggtcagcg ggatcacggg acgggccgcg ccgtccggcg gggcccaccg gccccgccca
     8881 cggcggtctc gacgaccgcc gtgggcggtt ccggcctcca ggtaccgtcc cgatcgcccc
     8941 cgacgacggg acggtactcc cccctcagga gtccagtgat cgcctgcatg gtctcccgtc
     9001 cggcttcgcc gccacccgtc tgcgccagat cgtaaaacgg ggtaaacaca ccgggatacc
     9061 gggcattcgg cgcggccgca ccgggggttc cggctccccg caccgggcat cggccggccc
     9121 gatcgcagct catgacgccg ccgcgcagag cgcgaggacc tcgttggtca gcgcggcggc
     9181 ggcgcccggc cggccgaggc ggcgcgccat cgccgacatc tccgagcggc gcagcggatc
     9241 ggtcagcagc agcgccagct ccccgaccag tcgcgcgggc gccgcctcct ccccgaccag
     9301 cacgcgggcg gcgccgtgcg cggcgaggtg ctcggcgttg cggcgccgct cgtccccggc
     9361 cgggcgggcg ggcagcacga gcaccgacgg cttgccgagc gcggtcagct cgccgaccgt
     9421 cccgcagccg ccgcccgaga ccacgacatc ggcgagggcg agcaggtcgg cgagctcctc
     9481 gccgacgaac ccgcgcgcga gataccgggg acgcaggtcg ggcggcagcc gctcgaccgc
     9541 cgccggcacc ggcccggccg gctccgcgcc cgcctggtgg aacacgttgg cgtagcgcag
     9601 cagctcgggc agggccgcgg tgaccagctc gttgatgtgg cgcgcgcccc gggagccgcc
     9661 ggtgacgtag acggtcggca gcgagggcgt ccagccgtcc cagcccagcc gtgcgggcgc
     9721 ggcccgcgcg tcgccgtccg cgaggcccgg acgcagcggg ttgccggtca ccaccgcgcg
     9781 cccgcgcagc cgctcgggga gcaggtccat ggtcggctcg gcgctgagcg cgacgcgcgc
     9841 cgcaccccgc atcgtgatcc ggttggcgag ctccacccgc accgcctgct cgtggacgag
     9901 cagcggccgg tggcagagcc gcgccgccag cccgaccggc accgacgcgt acccaccggt
     9961 gcacagcacg gcgtggggac gaatcccccg gacgatgttg accgcctccc gcaccccgac
    10021 cggcacccgg cccacggcgg acgacagccg ggagagccgg ttacttccgc ggaacgttcc
    10081 gccggtgggg atcgcggtga acggcacgcc ctcctccgcc gcgaccttgg cctcgacgcc
    10141 gcgctcgccg cccacccaga acggctggag ctccaggccg tgggcgcgga acgcggtccg
    10201 cgccgcccgc acgaccgcga cggcggggaa cgtgtggccg ccgctgccgc cgccgctgac
    10261 gacgagccgg accgggtcgc gccggtcgat cagggcttgg ggaacgacga catgcgtcat
    10321 gaccgctcca tgggacggaa atcggggctc aggcggagag ggcggcggcg agctggcggc
    10381 gcccggtgat gccgagcttg cggtaggcgc tggtgaggta ctgctcgacg gtgcgctggg
    10441 tcagcaccat ctcctcggcg atctggcggt tggtggcgcc ggaggcggcg cggtcggcgg
    10501 cggcgcgctc ctgcgcggtg agcgcgctga cgccgctgag ccgcccgagg tcgggcttgc
    10561 cgcccgcacg gcgcaggtgg cgggccgcgc gcagccagag cggccgcgcg ccgatctcgt
    10621 gggccagcgc gaacccgtcc tggaggcgct cgtgggcccg ctcggtgtcg ccgccgcgca
    10681 gcaggacggc gccgaggtcg atcagcgccg ccgcgcggtg cagcccggcg tcctccagcg
    10741 ggtcgaggac ggcgacggcc tcgcgcgccg cgtcggggtc cgcgttgacc acggcggacg
    10801 cggcgagcgc ggcgctcaac ggacgcggcg cgccccaccg gcgggccagc gcgagctcct
    10861 ccgcggc
//
