LOCUS       NZ_JAICDG010000004     21110 bp    DNA     linear   CON 29-AUG-2024
DEFINITION  Actinomadura terrae strain OS3-83 contig4, whole genome shotgun
            sequence.
ACCESSION   NZ_JAICDG010000004
VERSION     NZ_JAICDG010000004.1
KEYWORDS    WGS; RefSeq.
SOURCE      Actinomadura terrae
  ORGANISM  Actinomadura terrae
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Thermomonosporaceae; Actinomadura.
REFERENCE   1  (bases 1 to 942416)
  AUTHORS   Lee,S.D., Yang,H.L., Kim,I.S., Choe,H. and Kim,J.S.
  TITLE     Actinomadura terrae sp. nov. and Actinomadura litoris OS3-89,
            isolated from rhizosphere soil of cactus
  JOURNAL   Int J Syst Evol Microbiol 73 (1) (2023)
   PUBMED   36748505
REFERENCE   2  (bases 1 to 942416)
  AUTHORS   Lee,S.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-FEB-2022) Institute of Jeju Microbial Resources, BioPS
            Co., Ltd., Sancheondan Dongil 16, Jeju 63243, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 141x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022751215.1-RS_2024_08_29
            Annotation Date                   :: 08/29/2024 06:12:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,085
            CDSs (total)                      :: 8,002
            Genes (coding)                    :: 7,936
            CDSs (with protein)               :: 7,936
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 56 of 66
            Pseudo Genes (internal stop)      :: 3 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 21:31:58
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 710571
            Orig. end                         :: 731681
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAICDG010000004.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..21110
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="terpene"
                     /region_number="2"
                     /rules="(PT_phytoene_like or phytoene_synt or Lycopene_cycl
                     or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA
                     or TS_Pyr4)"
                     /tool="antismash"
     cand_cluster    1..21110
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /kind="single"
                     /product="terpene"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..21110
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="False"
                     /core_location="[10000:11110](+)"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..11110
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
     misc_feature    142..155
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 18.47"
                     /tool="antismash"
     misc_feature    complement(208..219)
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 18.46"
                     /tool="antismash"
     misc_feature    1744..1755
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: strong, score: 21.4"
                     /tool="antismash"
     gene            2140..3276
                     /locus_tag="K2F47_RS27895"
     CDS             2140..3276
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="K2F47_RS27895"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_242908491.1"
                     /transl_table=11
                     /translation="MSPNPLSRRTILTAGAAVAATPILGQTAASAASTPTWRDMPISLG
                     ADVASLNEVAVVNRRWGWAGGLLGGTAYNLALASWDGHAWSRFPIPEPFGGGGVVMAAD
                     RRHLWLMGYGTSYSAFWNGSTWRPAEIPTKPGFSFPIWVGPYSISAAPDGTAWAVLVDV
                     VHDLSLVLHWENGAWARADVPVPETGIAQQVAVRSSRDVWISGWYRDERQTRLPYSLHW
                     DGRSWKKVAVSPPDQPRESQSVKHILPVSRSLAWAYRNWDVMPSRRVLLRWTGGDSWQE
                     FPLPDDVPQVGPALADDGRHGVWIGGGGYGSTAYAHFRNGVWTVMRGPARDPQARVDAM
                     SLARVPGTRTIIAVGEILYSDAGSPTGYGKKPLTERLH"
     gene            3329..3862
                     /locus_tag="K2F47_RS27900"
     CDS             3329..3862
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="K2F47_RS27900"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_242908492.1"
                     /transl_table=11
                     /translation="MWIGGGALDGTGREYPLSIHWDGRSWTSVAVAPPDETRAAYRIRH
                     ILPISRSLAWAFAIAGPNGFPQTLLRWTGGSSWQEIPLPSGFSNANAPITDDGQGGVWL
                     GGDNPGAASYAHFRNGVWTVVYGPSRDPQDGVSVRGLSRAPGTRTVLATGEIIHRDPGT
                     PSGHGRKPFVERLH"
     misc_feature    3916..3929
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 16.71"
                     /tool="antismash"
     misc_feature    3938..3951
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 16.71"
                     /tool="antismash"
     gene            4001..4474
                     /locus_tag="K2F47_RS27905"
     CDS             4001..4474
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012855122.1"
                     /locus_tag="K2F47_RS27905"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="histidine phosphatase family protein"
                     /protein_id="WP_242908493.1"
                     /transl_table=11
                     /translation="MPTLIVLRHAKAVSGLEIPDIDRTLADRGRRDAAAAGEWLRANDL
                     VPDRVLCSVAVRTRETLDQLKIDAATTFEPKIYDNDPDELLALVAETPAEAGRLLLIGH
                     NPSVHHLVHDLTGRAPDAFPTCALAVIELPGAWADARPGTGTLTTVRTPKDED"
     gene            complement(4471..6702)
                     /locus_tag="K2F47_RS41535"
     CDS             complement(4471..6702)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024905.5"
                     /locus_tag="K2F47_RS41535"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="FUSC family protein"
                     /protein_id="WP_242908494.1"
                     /transl_table=11
                     /translation="MLKRARDAYAQVEGQIAPFYGLAAALAMALPLVAGSLTGRAAEGS
                     VIALGAYLVALRAPEGPYGARARDLAGGVVLVALGAGVGGALAGHTWPTVIVVPPLIAL
                     GVAVPRIGATAGLAVLLSAVRPPSADVVHTGVLELVGGLLTTALLLAPWPARRLRPLRE
                     ALFEAADAVALTLDAVAQDIGAPDESPLEDVHPDLLAVTRKPDWERCRRDASEALTAAR
                     GTYALYRTGRGGGDPTRPERLIDALSRVLHETVTLQAVLEAARNYPPDREWELEAQTAV
                     SALAARLRLLAGAIETWGEAPLGGEESAAVRRMGRAAETIRRAGLAGDEDLVAVSLIGQ
                     VRRSIDRIAGEVASARRIIAGGLRIGFGPPRMPDAPHPVSALERLRKAVRTRSPTFRQV
                     SRVFVTALVSMTLAAALHLPHGHWMTITAMLSLRASYGETVDRLVQRVGGTAAGSVVAA
                     LLLTLAPGRISTALLLSCFALLGFALRPVNFTYWALFGTPQAMLLLDFTVPADWHTAGE
                     RIGLTFAGTLLSFLAVSLLWPTGHRERLPVQLSRLLSLHADLVRAAADVTGGERVRLPH
                     EKIVAAEQAAEAVAETRTRLGHERVPDALLINRLDTVVRAAHRIRDHLIAVARISREKA
                     PDTGPVPEILDRLADTLEEAAAALKEPAEERELPSPVRELDDELAGLDDYLSTLTRRRR
                     AEIKSGVDRDELTPLRHALLQVSGIRYTIRALRRDTGRVIDGSFAAGSS"
     gene            complement(6813..8009)
                     /gene="serB"
                     /locus_tag="K2F47_RS27915"
     CDS             complement(6813..8009)
                     /EC_number="3.1.3.3"
                     /GO_function="GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="serB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HAD_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019631796.1"
                     /locus_tag="K2F47_RS27915"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="WP_242908495.1"
                     /sec_met_domain="HAD_2 (E-value: 0.00017, bitscore: 17.4,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDGPAQRTLLITLTGRDRPGVTSRLFRTLAEFPLTVADVEQVVIR
                     GRLVLGVLLAYTEGADIGRVWNAAEKVATDLDMEVELSTGRNKRMPKRRGRLHVTVLGA
                     PLAPAAMAGIASRIAANGANIDRIERLAATPVTCIELDVSGAVPDELRAGLAAEAAEQQ
                     VDIAVQRGGLHRRAKRLIVMDVDSTLIQGEVIELLAAHAGCLDEVAKVTEAAMRGELDF
                     EGSLRERVALLAGLDAGAIELVRGELQLAAGARTMVRTLKRLGYAFAIVSGGFTQVTDA
                     LVDDLGIDYSMANTLEIKDGKLTGGVTGPVIDRAGKAAALERFAEQAGVPLSQTVAIGD
                     GANDLDMLQAAGLGIAYNAKPVVREAADTTVNVPYLDTILFLLGISREEVEAADAGAY"
     misc_feature    complement(8211..8228)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 15.42"
                     /tool="antismash"
     misc_feature    complement(8457..8475)
                     /note="TFBS match to DmdR1, Iron(II)-dependent repressor,
                     confidence: weak, score: 24.42"
                     /tool="antismash"
     misc_feature    8479..8494
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: weak, score: 19.63"
                     /tool="antismash"
     misc_feature    complement(8489..8504)
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: weak, score: 19.57"
                     /tool="antismash"
     gene            8585..9952
                     /locus_tag="K2F47_RS27920"
     CDS             8585..9952
                     /GO_function="GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /GO_function="GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /GO_function="GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1034: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019050613.1"
                     /locus_tag="K2F47_RS27920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="cytochrome P450"
                     /protein_id="WP_242908497.1"
                     /sec_met_domain="p450 (E-value: 2.4e-76, bitscore: 253.0,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKSPDIPKARGALPLVGHAPRLFHRPHEFLTALPDYGDLVEIRLG
                     PVRAVVVCDPELTRQVLRGDRTFDKGGAIYRRLRELGGDGVITCTRDRHRRQRRLTGHA
                     LHSANLPGYAQTMTDQITSLTDRWADGQILDVPSEMLGFTMRVLTETMFSSALPQHALG
                     DTVDDLVVIMRGIFRRSVMPAPLNRIPTQGNRRYQRALTHLRTTLDATIADRRAAPPEG
                     EDLLSRLLSARDVADGGDGHGLTDREAADNVITFFVAGSETTANAVAWSLHLLARHPHI
                     EDQLYTEVRQVLGDGPARYEHLPHLGLTNRVIAETLRLYPPALMFSRITTTDTQLGRHR
                     ILAGTTIIFSPYIIHHRGDLHDNPESFDPSRWESTSQPPVSHYVPFGAGPRKCMGEQFG
                     ITEAALALATITARWHLESVPGRRVRTAMSLNPHGLRMRVVARTRDADSAAKTAKS"
     gene            10001..11110
                     /locus_tag="K2F47_RS27925"
     CDS             10001..11110
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: T1TS"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1052: Terpene synthase/cyclase metal-binding domain
                     protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF040195.4"
                     /locus_tag="K2F47_RS27925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_242908499.1"
                     /sec_met_domain="T1TS (E-value: 6.5e-58, bitscore: 192.8,
                     seeds: 237, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNVGQAPDLLAVRPFYCPIAPASSSWAAELNALAEDWLSIQGFEP
                     DEEQRKRLSRANLGELAARTMPGGHKNALANATRQYIAFFALDDACFDELPMPLSELTR
                     KQSIILSAIQHPEIRWPDEDAYVTASREIASGWKAIASPAQFQRWVEGMRAFFFGVTWE
                     AVYRHRDKLPGLNEYVAMWMRAIGMSPVLALVDTVGGYEVASSDLARPRVQALIEMAHF
                     LVAWDNDFISRAKEIRRAHDRLNIVDVIAAERGIEPAQALNAATAMRDRVMCLFLHTRD
                     QVTSSQDVHENLRRYAAGLAHYIRGHLDWALSCERYTNVRPTENAHREAPPSPWKDSPD
                     DDDFMRPLPIPAITWWWNPSVSSPLEDGR"
     misc_feature    complement(11173..11183)
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 18.59"
                     /tool="antismash"
     misc_feature    complement(12152..12170)
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 18.63"
                     /tool="antismash"
     misc_feature    12155..12173
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 18.49"
                     /tool="antismash"
     misc_feature    complement(12161..12175)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 18.26"
                     /tool="antismash"
     gene            12212..12652
                     /locus_tag="K2F47_RS27930"
     CDS             12212..12652
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1135: MarR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF013231.5"
                     /locus_tag="K2F47_RS27930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="WP_242908501.1"
                     /transl_table=11
                     /translation="MVVAALAKAVEHALLRVELTLPQYRMLSVLAREDSPTASRVAWEL
                     SVMPATVTAVMDGLVAGKYVRREPDPHDRRKIALVITSEGRHLLATANEEIGRRLGELT
                     SFLDGDQAKRAMDALALWREALASERQAEWVAREDARRKESR"
     gene            12649..14430
                     /locus_tag="K2F47_RS27935"
     CDS             12649..14430
                     /EC_number="7.-.-.-"
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="resistance (resist) ABC_efflux"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF012869.5"
                     /locus_tag="K2F47_RS27935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_242908503.1"
                     /transl_table=11
                     /translation="MSPPGAAIDPDPSRSWLRRAWPLVRAHKWMFGTALVSSGASTLVA
                     VQVPKVIQEALDNSVVSDRVPLAHYLWWLVGLTVVMLIVGFASKQLLFRAAARIEYDLR
                     NMVYEHLTNLSFPFYDRVQSGQLISRANSDIRTVQMYLGFAPYLLVQCGVSLVAFVYML
                     AINVPLAVLAMLPMPLFYVTGKRMQREMFPVSWLIQARLADVATVVDENVHGVRVVKSF
                     AAERNQLSLLQKAAARVRWAYVKDADLRARWTPLVQNLPSISMAIVLLYGGHLVIEGSL
                     GIGAIVAFSSYLLMLQVPFRVLGRLITFGQRASAAAKRLYEVLDERPEIVDAPGALVLT
                     EARGDVEFHDVAFGYGDGPDVLNGFSLRLRGGETVALVGRTGTGKSTVARLLPRFYDVR
                     EGSITVDGHDVRDLTLHSLRDTVGVVLDEPFLFSASVRDNIAYGRPDADIADIERAARL
                     AGAHGFIAELSEGYGTVIGERGYTLSGGQRQRIAIARTLLVNPPVLVLDDATSAIDVQV
                     EQEIHAGLRTLFAGRTTLIIAHRPSTIGLADRVVLLDGGRIVADGTHTELLASTPLYAE
                     VLAQADERVEALEGTGR"
     gene            14427..16346
                     /locus_tag="K2F47_RS27940"
     CDS             14427..16346
                     /EC_number="7.-.-.-"
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="resistance (resist) ABC_efflux"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF012869.5"
                     /locus_tag="K2F47_RS27940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_242908505.1"
                     /transl_table=11
                     /translation="MTFPGGGGGSGFGATSTGGGATASAHPFAGVPPEMRPGVEMLLKT
                     EPEHSEPEVVFEHARPDPDGARLSLRRLVRPYGTMALLAGVLVVVEALTLQAGPLLTQI
                     GIDDGITAKDFGVLVLVAAIYLVNLVVTGLTQAARVKVTGRVAAWVMNDLRVRVFTQLQ
                     RLSLNFYTGEKAGVIMTRMTADIENLQALLQEGLAQFAIQGLTMVIVSAVLFTYDVRLA
                     LITIVLVVPVMTAMSLWFRCASGKGYLRVRDATADVIADLSESLQGVRIVTAHNRQRRN
                     TEHHRRVTGRYFDASLQTGRINSIYTPGTQVIGVAGQIVLLAIGAGMVTDGRLTIGELV
                     AFLLYLGSFFQPIQQLVQQYSTYQQGQSSIVKLRQLLSTRPDVVEKPEAVDLPRVEGEV
                     IFEDVTFGYLPDRPVVSDVSLEIKAGETVAFVGPTGAGKSTLTKLITRFYDPGHGRILI
                     DGRDLRDVRIESLRRQLGVVPQEPFLFAGPLRDNIRFAIPDASDDQVREALRAVGLDDV
                     VDRLPDGLDTAVQERGQSMSSGERQLVALARAFLAQPRVLILDEATSNLDLQSEHKIEN
                     ALDVLLEGRTAILIAHRLSTAMKADRVVVVDEGRIVESGSHDQLIAAGGRYAQMYATWT
                     TEVT"
     gene            complement(18019..18912)
                     /locus_tag="K2F47_RS27945"
     CDS             complement(18019..18912)
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_ER (13-182). E-value: 1.1e-34.
                     Score: 111.9. Matches aSDomain:
                     nrpspksdomains_K2F47_RS27945_PKS_ER.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_175589223.1"
                     /locus_tag="K2F47_RS27945"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="WP_242908507.1"
                     /sec_met_domain="ADH_N (E-value: 1.7e-09, bitscore: 33.2,
                     seeds: 66, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKVIYAEFGGPDVLQLLDAEEPHAGPGEVRVAVRAAGVNPVDWR
                     LREGQVLGAHPVTLPAGVGLDAAGVVDEVGDGVEGVAVGDHVFGEGTDTYAEFSVLNAW
                     AQMPTGLTFEEAAGYPSVMETALRVLREVGVQPGQTLLVSGAAGGVGSAVLQIARERGI
                     TVIGTASPANQEYVRGLGAVATTYGAGWVERVRGLGKADAALDLAGAGVISELVELTGD
                     PRKVVSIADLDAPKLGVRFSNQAGSIRDALAETVRLISGGKLHIPVQKSYTLPEAAAAH
                     ADSRSGHTRGRRVILV"
     CDS_motif       complement(18328..18348)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_K2F47_RS27945_0003"
                     /evalue="3.60E+01"
                     /label="PKSI-ER_m2"
                     /locus_tag="K2F47_RS27945"
                     /protein_end="195"
                     /protein_start="188"
                     /score="-1.7"
                     /tool="antismash"
                     /translation="AGWVERV"
     aSDomain        complement(18367..18873)
                     /aSDomain="PKS_ER"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_K2F47_RS27945_PKS_ER.1"
                     /evalue="1.10E-34"
                     /label="K2F47_RS27945_PKS_ER.1"
                     /locus_tag="K2F47_RS27945"
                     /protein_end="182"
                     /protein_start="13"
                     /score="111.9"
                     /tool="antismash"
                     /translation="VLQLLDAEEPHAGPGEVRVAVRAAGVNPVDWRLREGQVLGAHPVT
                     LPAGVGLDAAGVVDEVGDGVEGVAVGDHVFGEGTDTYAEFSVLNAWAQMPTGLTFEEAA
                     GYPSVMETALRVLREVGVQPGQTLLVSGAAGGVGSAVLQIARERGITVIGTASPANQEY
                     VRGLGA"
     CDS_motif       complement(18439..18501)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_K2F47_RS27945_0002"
                     /evalue="1.50E-04"
                     /label="PKSI-ER_m5"
                     /locus_tag="K2F47_RS27945"
                     /protein_end="158"
                     /protein_start="137"
                     /score="14.4"
                     /tool="antismash"
                     /translation="GQTLLVSGAAGGVGSAVLQIA"
     CDS_motif       complement(18688..18723)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_K2F47_RS27945_0001"
                     /evalue="2.20E-02"
                     /label="PKSI-ER_m2"
                     /locus_tag="K2F47_RS27945"
                     /protein_end="75"
                     /protein_start="63"
                     /score="8.0"
                     /tool="antismash"
                     /translation="GLDAAGVVDEVG"
     gene            18983..19615
                     /locus_tag="K2F47_RS27950"
     CDS             18983..19615
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1215: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017573055.1"
                     /locus_tag="K2F47_RS27950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_242908509.1"
                     /transl_table=11
                     /translation="MTVPPGRRERKKAATRQKIADAAMRLFLERGYDAVGIRDVAAEAD
                     VAVTTLFSHFASKEALVFEQDQDFEQRLTRAVTDRDPHEPLIPALRREIEALLRHCGAD
                     SALPIWQMVDESPALRQYEGAMRLRHAESVATAIAGALDLPESTTSCRTIARFAIDAFS
                     LAREAADPDAALNEIFQMIEAAWEVSGLPWHGRGTDKAEAGQAVTSS"
     misc_feature    19741..19756
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 17.75"
                     /tool="antismash"
     misc_feature    20067..20081
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 16.89"
                     /tool="antismash"
     gene            20281..20430
                     /locus_tag="K2F47_RS27955"
     CDS             20281..20430
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF016001.5"
                     /locus_tag="K2F47_RS27955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="WP_242908815.1"
                     /transl_table=11
                     /translation="MYVLNLHTSGCRSGRPRETPLAWCADGDDARLVVASGGGGVGTPT
                     GMPI"
ORIGIN
        1 ctggtcgcgg cgctgatcct cggcaccgcg gcgttcggca ccgagggcct ggtgttcccg
       61 ctgatcgtgc cgatgatcgg cgtgatcacc gcggtcatcg ggatcttcgc cgtcgcgccc
      121 cgggacggtg accggtccgg gatgtccgcg atcaaccggg gcttcttcat ctcggcgatc
      181 atctcggcgg tgctggtggc gatcgcggcg ttcgcctacc tgccctcgtc gttcgccgac
      241 ctgtccggcg tcaccgaccc cgacatccgg aacctggacg ccgatccgcg ctgggtcgcg
      301 ctcggcgccg tgatcatcgg gatcgtgctg gcgagcgcga tccagctgct caccggctac
      361 ttcaccgaga cgaaccgcaa gcccgtccag gaagtgacca acagtgccag gacgggcccg
      421 gcgaccgtca tcctgtccgg tatctcgctc gggctggagt cggccgtcta caccgcgctc
      481 gtgatcggcg cggcggtgta cggggcgttc ctgctcggct tcggcaacac caccgtggcg
      541 ctgttcgcgg tggcgatggc cgggaccggg ctgctgacca cggtcggcgt catcgtctcg
      601 atggacacct tcggcccggt gtcggacaac gcgcagggca tcgccgagat gtccggcgac
      661 gtccagggcg aggccgcgtc ggtgctggag cggctggacg cggtcggcaa cacgaccaag
      721 gcgatcacca agggcatcgc gatcgcgacg gcggtgctgg ccgcgaccgc gctgttcggc
      781 tcgttccgga cgacggtcgt cgaagcgctc ggcgacgcct cggccgacgc caaggacgcg
      841 ctcggcgact tcaaggtgtt cagcctgtcg atcgacaacc cgaacgtgct gatcgggctg
      901 atcatcggcg cggccgtcgt gttcctgttc tccggcctgg cgatcatggc ggtcggacgg
      961 gcggccggac gggtcgtggt ggaggtgcgc gagcagttcc gcaccaagcc cgggatcatg
     1021 aactacaccg agaagcccga ctacgaccgg gtcgtggaca tctgcacccg ggacgcgcag
     1081 cgcgaactcg ccacccccgg cctcctggcg atcatgacgc cgatcacggt cggcttcgcg
     1141 ctcgggtacg cgccgctcgg cgccttcctc ggcggcgcca tcgcggccgg ggtgctgatg
     1201 gcggtgttcc tgtccaactc cggtggtgcc tgggacaacg ccaagaagct cgtcgaggag
     1261 gggcacctcg gcggcaaggg cagccaggcg cacgaggcga cggtgatcgg cgacacggtc
     1321 ggcgacccgt tcaaggacac cgccggtccc gcgatcaacc cgctgatcaa ggtgatgaac
     1381 ctggtcgcgc tgctgatcgc ggacgccgtc gtcacctacg cgggcaacgt cccgctgcgg
     1441 atcggcgtga cggtcgtgtc ggtcggcatc gtggtggcgg cgatcgtggt gtccaagcgg
     1501 cgcgccgacc cgatcgccga gaccccggcc acgccgccgg ccgcggaggg cccggggacg
     1561 tccgggtccc tcggcggcgc gtcggtcgtc gagccgcgca acggcgagac cgaggccgag
     1621 cccgcgggtg aggggtcggc ggccaacaag ggctgaccgt cctggatagg cgagatcgag
     1681 ggccggtggc gcgcgaggcg gcaccggccc tctgtcgtgg gcacgcgggg gtggtcatac
     1741 gctgtacgtg aacgcgccgg gacgacggcc ggccgacgaa gcgtggaggt ggacggtggc
     1801 gagcggaggg aagtcggcga tgtccgggcc caaggggttc gcgctcgtgc tcggcgggat
     1861 gctcggcatc atgctcctgc tctggggcct cgcggcactc gtcgccccct gaccccccaa
     1921 gcggttcccc cggcacgaac actcctgaca aaacctccat tcggcttgtc cgtttttgga
     1981 gggcggccga gcttcgcagg tgaagcccgg tttacgcggg ttgccgtttc cggccgtgcc
     2041 ggatgcggac cttgaggagc cgccggaacg gcgggcaccg tggcgggtga cggtccccgg
     2101 tcgcgggtga tgctcccccg tccgacctca ggagcccgga tgtcgcccaa tcccctcagc
     2161 cgccggacca tcctcacggc cggtgccgcc gtcgccgcca cccccatcct cggccagacg
     2221 gccgcctcgg cggcatccac cccgacctgg cgagacatgc cgatatccct cggggcggac
     2281 gtcgcctccc tcaacgaagt cgccgtcgtg aaccgccgct ggggatgggc agggggcctc
     2341 ctaggaggga cggcctacaa cctcgccctg gccagctggg acggacatgc ctggagccgt
     2401 ttccccatcc cggagccctt cggcggagga ggcgtcgtca tggcggcgga ccgtcgccac
     2461 ctgtggctca tgggctacgg caccagctac agcgccttct ggaacggttc cacctggcgc
     2521 ccggcggaga tcccgaccaa gcccggtttc agcttcccca tctgggtcgg cccgtactcg
     2581 atctcggcgg cgcccgacgg gacggcctgg gcggtgctcg tggacgtggt gcacgacctc
     2641 tccctcgtcc tgcactggga gaacggcgcg tgggcccggg cggacgtgcc ggtgcccgag
     2701 acggggatcg cgcagcaggt cgcggtccgg tcgtcccgcg acgtgtggat cagcgggtgg
     2761 taccgcgacg agaggcagac ccggctgccc tacagcctcc actgggacgg gcgctcctgg
     2821 aagaaggtgg ccgtctcgcc gcccgaccag ccccgcgaga gccagagcgt caagcacatc
     2881 cttccggtct ccaggtcact ggcctgggcc tatcggaact gggacgtgat gccctccagg
     2941 cgggtcctgc tccgttggac gggcggcgac tcctggcagg agttcccgct ccccgacgac
     3001 gtcccccagg tcggcccggc cctcgccgac gacggccggc acggtgtgtg gatcggcggc
     3061 ggaggctacg ggtccaccgc ctacgcgcac ttccggaacg gcgtctggac ggtcatgcgc
     3121 ggccccgcac gcgacccgca ggccagggtc gacgccatga gcctggccag ggtgcccggc
     3181 acgcgcacga tcattgcggt gggcgagatc ctctactccg acgccggatc tccgaccgga
     3241 tacgggaaga agcccctcac tgagcgcctc cactaacgcg cccccagcta tcatcgctcg
     3301 gatcgtcgcc gtccggtcgt cccgtgacgt gtggatcggc gggggagccc tcgacgggac
     3361 gggaagggag tacccgctca gcatccactg ggacggccgc tcctggacga gtgtcgccgt
     3421 cgcacccccc gacgaaaccc gcgcggctta ccgcatcaga cacatcctcc cgatctccag
     3481 gtcactggcc tgggccttcg cgatcgccgg accgaacggc ttcccccaga ccctgctccg
     3541 ttggacgggt gggagttcct ggcaggagat cccgctcccc tcggggttct ccaacgccaa
     3601 cgcgcccatc acggacgacg gccagggcgg cgtgtggctc ggcggcgaca acccgggcgc
     3661 cgccagctac gcgcacttcc ggaacggcgt ctggacggtc gtgtacggcc cctcgcgcga
     3721 cccgcaggac ggggtcagcg tcaggggtct gtccagggca cccggtacgc ggacggtcct
     3781 ggcaacgggc gagatcatcc atcgcgaccc cggaaccccg tccggccacg gaaggaagcc
     3841 gttcgtcgag cgcctgcact aacgcgggtt acgggcgcat tcaccaatcg cagtcgcatt
     3901 tcctaacacc tcaaccgcat acgcgtgcaa cctcaggcgc atacgcgtgc agccgacagg
     3961 tcgctgccgg ccaagacggg acaacaccta gggtggcgcc atgccgacgc tcatcgtgct
     4021 ccgccacgcc aaggccgtct ccggcctgga gattcccgac atcgaccgca ccctcgccga
     4081 ccgcggacgg cgcgacgccg ccgcggccgg ggagtggctg cgcgcgaacg acctcgtccc
     4141 cgaccgggtg ctgtgctcgg tcgcggtacg gacgcgcgag accctcgacc aactcaagat
     4201 cgacgcggcg acgacgttcg agccgaagat ctatgacaac gaccccgacg agctgctcgc
     4261 gctcgtggcc gagacccccg ccgaggcggg caggctcctg ctgatcgggc acaacccgtc
     4321 ggtgcaccac ctcgtccacg acctgaccgg gcgggcgccg gacgcgttcc cgacctgcgc
     4381 gctcgcggtg atcgagctgc cgggcgcgtg ggcggacgca cgtcccggca ccggaacgct
     4441 gacgaccgtc cgaaccccga aggacgagga ctaggacgac ccggccgcga acgatccgtc
     4501 gatgacgcgt ccggtgtcgc ggcgcagcgc cctgatcgtg tagcggatgc ccgacacctg
     4561 gaggagggcg tgccggagcg gcgtcagctc gtcccggtcg acgcccgact tgatctcggc
     4621 gcggcggcgg cgggtgagcg tggagaggta gtcgtcgagc ccggcgagct cgtcgtccag
     4681 ctcccggacc ggggacggga gctcgcgctc ctccgccggt tccttgagcg cggcggcggc
     4741 ctcctccaac gtgtccgcga ggcggtcgag gatctcgggg acgggcccgg tgtcgggcgc
     4801 cttctcgcgg gagatccgcg cgaccgcgat caggtggtcg cggatccggt gcgcggcgcg
     4861 gacgacggtg tccagcctgt tgatcagcaa cgcgtcgggg acccgttcgt ggccgagccg
     4921 ggtccgggtc tcggcgaccg cctcggccgc ctgctcggcg gcgacgatct tctcgtgcgg
     4981 gagccggacg cgttcgccgc cggtcacgtc cgccgccgcc cggacgaggt cggcgtgcag
     5041 cgacagcagc cgcgacaact ggacgggcag ccgctcgcga tgaccggtcg gccacagcag
     5101 cgagaccgcg aggaacgaca gcagcgtccc ggcgaaggtg agcccgatcc gctcgcccgc
     5161 ggtgtgccag tcggccggga cggtgaagtc cagcagcagc atcgcctgcg gcgtcccgaa
     5221 caacgcccag taggtgaagt tcaccggacg caacgcgaac cccagcagcg cgaagcagga
     5281 gaggagcagc gcggtggaga tgcgccccgg cgcgagcgtg agcagcagcg ccgcgacgac
     5341 cgagcccgcg gccgtgccgc ccacgcgctg gacgagccgg tcgacggtct cgccgtagct
     5401 ggcgcgcagg ctgagcatcg ccgtgatcgt catccagtgc ccgtgcggca ggtgcagcgc
     5461 ggcggccagc gtcatcgaca ccagcgccgt cacgaacacg cgcgagacct gccggaacgt
     5521 cggcgaccgc gtccggacgg ccttgcgcag ccgctccagc gccgagacgg gatgcggcgc
     5581 gtccggcatc cgcggcggac cgaacccgat gcgcagcccg cccgcgatga tccgccgcgc
     5641 ggacgcgacc tcaccggcga tccggtcgat cgaccgccgc acctggccga tcagcgagac
     5701 cgcgacgagg tcctcgtccc cggcgagccc ggcgcgcctg atcgtctcgg cggcccgccc
     5761 catccgccgg acggccgccg actcctcccc tcccagcggc gcctcgcccc acgtctcgat
     5821 cgcgcccgcc agcagccgca gccgcgccgc cagcgcggac acggcggtct gcgcctccag
     5881 ctcccactcg cgatccggcg ggtagttccg cgcggcctcc agcacggcct gcaacgtcac
     5941 ggtctcgtgc aggacgcgcg acaacgcgtc gatcaaccgc tcgggacgcg tggggtcgcc
     6001 gccgccgcgt cccgtccggt acagggcgta ggtgccgcgc gccgcggtga gggcttcgga
     6061 cgcgtcgcgc cggcaccgct cccagtcggg cttgcgggtg acggcgagca gatcggggtg
     6121 gacgtcctcc aaggggctct cgtcgggggc gccgatgtcc tgcgcgacgg cgtccagcgt
     6181 cagcgcgacc gcgtctgcgg cctcgaacag cgcctcccgc agcggccgca gccgccgcgc
     6241 gggccagggc gcgagcagca gcgcggtcgt gagcagcccg ccgaccagct ccagcacgcc
     6301 ggtgtggaca acatcggcgg acggcgggcg gaccgcgctc agcagcaccg cgagccccgc
     6361 cgtcgccccg atccgcggga ccgcgacgcc cagcgcgatc agcggcggca ccacgatcac
     6421 cgtgggccag gtgtgcccgg cgagcgcccc gccgaccccc gcgccgagcg cgaccaggac
     6481 gacgcccccg gccaggtcgc gggcccgcgc cccgtacggc ccctccggcg cccgcagcgc
     6541 gaccaggtac gcgccgagcg cgatcaccga gccctccgcc gcacgcccgg tcagcgaccc
     6601 ggcgaccagc ggcagcgcca tcgcgagcgc ggcggccagc ccatagaagg gcgcgatctg
     6661 cccctccacc tgggcgtacg cgtcccgcgc acgcttgagc accctgcctc cgctgtccgg
     6721 cgacccgcta tctgatcacc aataccccga ccgggaccca gtaggctccg gtaccccacg
     6781 gggactcagg aggctcgggt gccccagggg actcagtagg ccccggcgtc cgccgcctcg
     6841 acttcctcgc gcgagatgcc gagaaggaac aggatcgtgt cgagataggg cacgttgacc
     6901 gtcgtgtccg cggcctcgcg gacgacgggc ttggcgttgt aggcgatccc gagcccggcc
     6961 gcctggagca tgtcgagatc gttcgcgccg tcgccgatcg cgaccgtctg gctgagcggc
     7021 accccggcct gctcggcgaa gcgttcgagc gcggcggcct tgcccgcccg gtcgatcacg
     7081 ggcccggtga cgccgccggt gagcttgccg tccttgatct ccagggtgtt ggccatcgag
     7141 tagtcgatgc cgagatcgtc gaccagagcg tcggtgacct gcgtgaaccc gccgctgacg
     7201 atcgcgaacg cgtacccgag ccgcttcagc gtgcggacca tcgtccgcgc cccggccgcg
     7261 agctggagct cgccccggac cagttcgatc gcgccggcgt cgagccccgc cagcagcgcg
     7321 acccgctcgc gcagcgaccc ctcgaagtcc agctcaccgc gcatggcggc ctcggtgacc
     7381 ttggcgacct cgtccagaca ccccgcgtgg gcggcgagca gctcgatcac ctcgccctgg
     7441 atgagggtgg agtcgacgtc catgacgatc agccgcttcg cccgccggtg caggccgccg
     7501 cgctggaccg cgatgtcgac ctgctgctcg gccgcctcgg cggcgagccc ggcccgcagc
     7561 tcgtcgggga ccgcgccgga gacgtccagc tcaatgcagg tgacgggggt cgcggcgagc
     7621 cgctcgatcc gatcgatgtt ggcgccgttg gcggcgatcc ggctggcgat ccccgccatc
     7681 gcggccggtg cgagcggcgc gccgaggacc gtgacgtgca gccgtccgcg ccgcttcggc
     7741 atccgcttgt tgcggccggt ggacagctcg acctccatgt cgaggtccgt cgcgaccttc
     7801 tcggcggcgt tccacacccg tccgatgtcg gcgccctcgg tgtaggcgag cagcacgccg
     7861 agcacgagtc ggccgcggat cacgacctgc tccacgtcgg cgaccgtcag cgggaactcc
     7921 gcgagggtac ggaacagccg agacgtgacg ccggggcggt cacgtccggt cagggtgatg
     7981 agcagcgtgc gctgtgctgg gccgtccatg ctgttcacaa cggtaccggg cgggacgtct
     8041 ggatcggctg tgacgtccac catgcaagac gtcgggtgcg tgacaaggtc tttacgaccg
     8101 cggaaccagg aggttgaccg cgcctccgaa ctcggatgga tccccctgtc aaccccacgg
     8161 caggacccac cccctcaagc cggacgagca cttcgcatcg ttccggccat ataacccaac
     8221 cctaaccata gtttgagaca cccccacaag cccgccgccc ccgcccccaa cgaccagtcc
     8281 ccgcggtcac agtggccacc gcgcacgccc gcgaccaggt gaccagatca acgcggcgga
     8341 cacgacgctc cctgacaccc ctcaaaccac actgaaccct cggcgcctcg ggaggccgag
     8401 gactgcgacc agcgcggcgg ccgcactcgt catgcagcag gaatctcgcc cttgtgtcag
     8461 gcgggcctta ccttaccgat aggtcgggac cggtcccgac ctgtcggtgt aacactcggc
     8521 atagctcgcg atattcgcca tctccacggg tggagagcgg cctaacagtt cggacggagt
     8581 agccatgaag tctccggaca tccccaaggc gcgcggtgcc ctgccgctgg tcgggcatgc
     8641 gccgcggttg ttccaccggc cccatgaatt cctcaccgcg ctacccgact acggagatct
     8701 cgtcgaaatc cgtctgggac ctgtcagggc ggtggtggtc tgcgatcccg aactgacccg
     8761 ccaagtactg cggggagacc gcaccttcga caaaggcggt gccatctaca ggcgcctgcg
     8821 cgaattgggc ggcgacggtg ttatcacctg cacacgcgat cggcatcgga ggcagcggcg
     8881 cctgaccggg catgcgcttc acagcgccaa cctccccggt tacgcgcaga ccatgaccga
     8941 tcagatcacc tcgctcaccg atcgttgggc ggacgggcag atcctcgacg tgccatccga
     9001 gatgctcggg ttcaccatgc gggtgctgac ggagacgatg ttctccagcg cactaccgca
     9061 gcacgccctc ggcgacacgg tcgacgacct cgtcgtgatc atgaggggaa tcttccggcg
     9121 gtcggtcatg cccgcgccgc tgaaccggat tccgacccag ggcaaccgtc gctaccaacg
     9181 agcgctcacc cacctgcgga ccacactcga cgcgaccatc gccgaccgcc gcgccgcgcc
     9241 gcccgaaggc gaggacctgc tgtcccggct gctgtcggca cgcgacgtcg ccgatggcgg
     9301 cgacggccac ggcctgaccg accgggaggc cgccgacaac gtcatcacct tcttcgtcgc
     9361 cggttccgag accaccgcga acgctgtggc ctggtccctg catctgctcg cccggcatcc
     9421 gcatatcgaa gaccagctct acaccgaagt gcgtcaggtg cttggagacg gccccgccag
     9481 atacgaacac ttgccgcacc tcggcctgac caatcgcgtc atcgccgaga ccctgcgcct
     9541 ttatccgccc gcactgatgt tctcccgtat caccaccacc gacacccaac tcggccgaca
     9601 tcggattctg gcaggcacca cgataatctt ctccccatat atcatccacc atcgcggcga
     9661 cctccacgat aaccccgaga gcttcgatcc aagccggtgg gaatccacgt cgcagccgcc
     9721 ggtcagccac tacgtaccgt tcggcgcggg accccgaaag tgcatgggag agcagttcgg
     9781 aataaccgag gccgccctcg ctctcgcgac catcaccgcc cgctggcacc tcgaatccgt
     9841 gcccggccgc cgcgtccgca ccgccatgtc cctgaacccg cacggcctcc ggatgcgagt
     9901 cgtcgcgcga acccgggacg ccgatagcgc cgccaaaacg gccaagtcat aatctcctcg
     9961 cggccaggca gcgcgaaaac aggaaactcc ggagggatca gtgaacgtcg gccaggcccc
    10021 agacctactc gccgtacgac cattttactg cccgatagcg cccgcctcct catcctgggc
    10081 cgccgaactc aacgccctgg cagaggactg gctttcaatc cagggattcg aaccggatga
    10141 ggaacagcgg aaacgcctct cacgcgcgaa cctcggagag ctcgccgcca gaaccatgcc
    10201 gggcggccac aagaacgcac tagcgaacgc cacccgccag tacatcgcat tcttcgccct
    10261 ggacgacgcc tgcttcgacg aactccccat gccactgagc gagcttacga gaaaacagtc
    10321 gatcatcctc agcgccatcc agcatccgga gattcgttgg cccgacgaag acgcgtatgt
    10381 aacagcatcg cgcgagattg caagcggatg gaaggcaatt gcttcccccg cgcagttcca
    10441 gcgctgggtc gagggaatgc gcgcgttctt cttcggtgtt acctgggaag cggtctaccg
    10501 tcacagagat aaacttcctg gcctcaatga gtatgtcgcc atgtggatgc gcgcgatcgg
    10561 catgagcccg gttctcgccc tcgtcgacac agtcggcggg tacgaggttg catcctccga
    10621 cctcgcacgt cccagagtac aagccctgat agaaatggct cacttcttgg tggcgtggga
    10681 caacgacttc atctcccgag ccaaggaaat acgacgggcc cacgaccgcc tcaacatcgt
    10741 cgacgtcatc gcagccgaaa gaggcatcga acctgcccaa gcgctaaacg cggccacggc
    10801 gatgcgcgat cgagtaatgt gcctgttcct gcacacgcgc gaccaggtga cgtcgtccca
    10861 agacgtccac gagaacctac gaagatacgc agccggcctg gcgcattaca tacgaggcca
    10921 tctggattgg gcattgtcgt gcgagagata caccaacgtc cgtcccaccg aaaatgccca
    10981 ccgggaagcc ccgccgtccc cctggaaaga cagcccggac gacgacgact tcatgaggcc
    11041 gctgcccatc cccgccatca cctggtggtg gaacccctcg gtgagctctc cccttgaaga
    11101 tggccgctaa ccagggttaa taagtcatgg cggttgggtg ggtacaccgc ctggcagcgg
    11161 gggcgtatgc cggtgacatg gccagatatc cccagggggc gggatgaccg acaccgggca
    11221 tgcgtgtcga gtgcgggtcc ggttgcaggc ggcgcagtga tttgctgagg gtcgtccgcg
    11281 tcgccgtcca cgggcacgca cgggcgaccg ggaccgggcg aggcatagtt cgccaagctg
    11341 gaagccgact tgaaccgtgg cctggctgca cacggctacg ccgatgacca gccgtggacg
    11401 cttggcctgg tgacagtgct cataaaagag ctgttcggca tcgactacac cttgcccgag
    11461 gtgtcgctgc cgccgcgccg taacaggtgg tcggtgcagg ttcctgggca cggtccgtcc
    11521 aacgcgacga gcaggccatc accgcaggcg accttggtgc ggacaccggc cgcaggtgag
    11581 ggtgcacggt ggccagcacg gcatctgggt ctcggtagcc gggatggtct gccgctggtc
    11641 gcgacggcgg taggcggcgg atccacaggc ttgtgagtag tagatggcat cggctcgctg
    11701 gcgaacgccc tggtggagaa cctgacggca gagcgggcag tgcggcccgt ccggccggta
    11761 gtcctccggg agcgcgccct cacgacgcaa ccggtcagcg gcgaaccctg tctccaggat
    11821 gcgggcgtgc agctctcgct tccgcgcgcc ccgcacgcgc gcccgacagg ctcgggcggc
    11881 acaggcgtgt cgcactaccg ccgacgaggg ttggcgccac ggggcaacgg cttcccacag
    11941 ccctcacgac gtccacgacc ttccgaccac gctcaagccc gacgagcacc gcccctgacg
    12001 cacgccagaa cacgcagcgg cccttggcta ggtcgggagg ccggggcttc ggccgtccat
    12061 cccgcgtgtg cgggcacgac aggccgaagc gtcgatgccg aactccccaa acacaccgac
    12121 aagcggcgcg ctcgtaggcc gctcggtact gcggacggtg tattcagttt gataatctaa
    12181 atgtgtgtct cccgaagcag ttgagccggg cgtggtcgtg gccgcgctgg ccaaggccgt
    12241 tgaacatgct ctgctccgtg tggagctgac gttgccgcag taccggatgc tgtccgtgtt
    12301 ggcgcgggag gattcgccga cggcgtcgcg ggtggcgtgg gagttgtcgg tgatgccggc
    12361 gaccgtcacc gcggtcatgg acggcttggt ggcgggcaag tacgtgcggc gcgagcccga
    12421 tccccatgac cgacgcaaga tcgcactcgt gatcacatcc gagggtcggc acctgctggc
    12481 cacggccaac gaggagatcg gacggaggct aggcgagctg acctcgttcc tcgatgggga
    12541 ccaggccaag cgggcgatgg acgcgttggc gctgtggcgc gaggcgctgg cgagtgagcg
    12601 gcaagcggag tgggtcgccc gggaggacgc gcggcggaag gaatccaggt gagcccgccg
    12661 ggtgcggcga tcgacccgga tccgagtcgt tcctggctgc ggcgggcgtg gccgctggtc
    12721 cgggcgcaca agtggatgtt cgggacggcg ctggtgtcgt ccggggccag cacgttggtg
    12781 gccgttcagg tgccgaaggt gatccaggaa gcgctcgaca actcggtcgt ctcggaccgg
    12841 gtgccgctgg cgcactacct gtggtggctg gtcgggctga cggtggtcat gttgatcgtc
    12901 ggcttcgcgt ccaagcagct gctgttccgc gccgcggcca ggatcgagta cgacctgcgc
    12961 aacatggtgt acgagcacct gaccaatctg tcgttcccct tctacgaccg ggtgcagagc
    13021 gggcagctga tctcccgcgc caactccgat atccgcacgg tgcagatgta tctggggttc
    13081 gcgccgtatc tgctggtgca gtgcggcgtc tcgctggtgg cgttcgtgta catgctggcg
    13141 atcaacgtgc cgctggccgt cctggccatg ctgccgatgc cgttgttcta tgtgaccggc
    13201 aagcgcatgc agcgggagat gttcccggtg tcgtggctga tccaggcacg gctggccgac
    13261 gtggcgaccg tggtggacga gaacgtccac ggcgtccgcg tggtgaagtc gttcgcggcc
    13321 gagcggaacc agctgtcgct gctccagaag gcggcggcga gagttcgctg ggcctatgtg
    13381 aaggacgccg acctgcgcgc gcggtggacg cccttggtgc agaacctccc cagcatcagc
    13441 atggcgatcg tcctgctgta cggcgggcac ctcgtgatcg agggcagcct gggcatcggc
    13501 gcgatcgtgg ccttcagctc gtacctgctg atgctccagg tgccgttccg ggtgctcggc
    13561 aggctgatca cgttcgggca gcgggcgtcg gccgcggcga aacggctgta cgaggtgctg
    13621 gacgagaggc cggagatcgt cgacgccccc ggggcgctgg tgctcaccga ggcgcgcggc
    13681 gacgtcgaat tccacgacgt cgccttcggc tacggcgacg ggcccgacgt gttgaacggg
    13741 ttctcgctgc ggctgcgcgg cggcgagacg gtcgcgctgg tcggacggac cggcaccggg
    13801 aagtccaccg tggccaggct gctgccgcgg ttctacgacg tgcgcgaggg ttcgatcacc
    13861 gtggatggac acgacgtccg ggacctgacg ctgcacagcc tgcgcgacac cgtcggcgtc
    13921 gtcctggacg agccgttcct gttctcggcg tcggtccgcg acaacatcgc ctacggacgg
    13981 ccggacgccg acatcgccga catcgagcgg gcggcccggc tggccggggc gcacgggttc
    14041 atcgccgaac tgtccgaggg gtatggcacc gtcatcggtg aacgcggcta cacgctgtcg
    14101 ggcgggcagc ggcagcgcat cgcgatcgcg aggacgctgc tggtgaaccc gccggtgctg
    14161 gtgctcgacg acgccacaag cgccatcgac gtccaggtgg agcaggagat ccacgccggg
    14221 ctgcggacgc tgttcgccgg ccggacgaca ctgatcatcg cgcaccggcc gtccacgatt
    14281 ggcctggccg accgcgtggt gctgctggac ggcggccgga tcgtcgccga cggcacgcac
    14341 accgaactgc tggcgagcac gccgctctac gcggaagtgc tcgcgcaagc ggacgagcgc
    14401 gtcgaggcac tggaagggac gggccgatga cgttccccgg cggcggtggc gggagcggtt
    14461 tcggcgcgac gtccaccggc ggcggcgcga ccgcgtcggc gcacccgttc gccggtgtgc
    14521 cgccggagat gcggccgggt gtcgagatgt tgctcaagac agagccggaa cactccgagc
    14581 ccgaggtcgt cttcgagcac gcccggcccg atcccgacgg cgcgaggctg agcctgcggc
    14641 gcctggtccg tccgtacggg acgatggcgc tgctggccgg cgtgctcgtg gtcgtggagg
    14701 cgctgacgtt gcaggccggt ccgctactga cccagatcgg catcgacgac ggcatcacgg
    14761 cgaaggactt cggcgtcctg gttctggtcg cggcgatcta cctggtcaac ctggtggtca
    14821 ccggcctcac gcaggccgcg cgggtcaagg tcaccgggcg ggtcgccgcg tgggtgatga
    14881 acgacctgcg cgtacgggtc ttcacccagt tgcagcggct gtcgctgaac ttctacaccg
    14941 gggagaaggc cggcgtgatc atgacccgga tgaccgccga catcgagaac ctccaggcgc
    15001 tgttgcagga gggcctggcg cagttcgcca tccagggcct gaccatggtc atcgtctcgg
    15061 cggtgctgtt cacctacgac gtgcggctgg cgctcatcac gatcgtgttg gtcgtgcccg
    15121 tcatgaccgc gatgtcgctc tggttccgct gcgcgtccgg caagggatac ctgcgggtac
    15181 gggacgccac cgccgacgtg atcgccgacc tctccgagag cctgcaaggc gtgcgcatcg
    15241 tcaccgcgca caaccggcag cggcgcaaca ccgagcacca ccggcgggtc accggcaggt
    15301 acttcgacgc cagcctccag accggacgga tcaattcgat ctacacgccc ggcacccagg
    15361 tcatcggcgt cgccgggcag atcgtcctgc tggccatcgg cgcgggcatg gtcaccgacg
    15421 gaagactgac catcggcgag ctggtcgcgt tcctgctcta cctggggtcg ttcttccagc
    15481 ccatccagca actggtgcag cagtacagca cctaccagca gggacagtcg tcgatcgtga
    15541 agctgcggca actgctgagc acccgtccgg acgtcgtgga aaagcccgaa gccgtcgacc
    15601 tgccgcgcgt cgagggcgag gtcatcttcg aggacgtgac gttcggctat cttcccgacc
    15661 ggccagtggt ctccgacgtg tcgctggaga tcaaggccgg tgaaacggtg gcgttcgtcg
    15721 gcccgaccgg cgcgggcaag tccaccctca ccaaactgat cacccggttc tacgacccgg
    15781 gccacggccg gatactcatc gacggacgcg acctccggga cgtccggatc gaatccctgc
    15841 gcaggcagct cggcgtcgtg ccgcaggagc ccttcctgtt cgccgggccg ctccgggaca
    15901 acatccgctt cgcgatcccg gacgcctccg acgaccaggt gcgggaggcg ctgcgtgcgg
    15961 tcggcctgga cgacgtggtc gaccggctgc ccgacggcct cgacaccgcc gtccaggagc
    16021 ggggccagtc catgtcgtcc ggtgaacggc agctcgtcgc cctggccaga gcattcctgg
    16081 cccagccccg ggtgctgata ttggacgagg ccacgtccaa cctcgacctc cagtccgagc
    16141 acaagatcga gaacgccctg gacgtgctgc tggagggccg caccgcgatc ctgatcgcgc
    16201 accgcctctc gaccgccatg aaggccgacc gcgtcgtcgt cgtggacgag ggccggatcg
    16261 tcgagtccgg ctcgcacgac caactcatcg ccgccggcgg caggtacgcc cagatgtacg
    16321 ccacctggac caccgaggtc acctgacgaa tcccgagtga tcagctcggg ctcgaagagg
    16381 acagaagacc ctcgaccaga tcgacgtcag gggacgtcag agccgtacag cgaacggacc
    16441 cggccgcgga gccacgaaat atcgaccaac tccatctggg acgcggggta ctcatccatc
    16501 ggcgttcggc ccgccccgcc gccgacgaac acgccaagcg gcagcgcggc cgcaacccac
    16561 cacgcaggga gcgggcgcac gcttggcgca cgtagcgcag ccggacgtcc aacatagggg
    16621 cgttgtcgaa cgtcccgcag tcagggcgca accgctcgcc agcgcgcgga gcaccgtcac
    16681 acgaccgcac gcccgataca cagagcgcag tcgaacgccc agcagacagg gcgcgggcac
    16741 tcgtccagcg tgcggggccg ccgcacgcaa gccgcgaagc acggccgcac gcttaccgcg
    16801 tggaacgcag ccggacgtcc cacacgggag cacggtcaca cgcccggccc gcaaggcgca
    16861 gtcaagcgtc caccccgcgg cgcccggcca cacgcatcac gcggggccgc cgcacgcaac
    16921 acccggaaca cgcccgcgcg cccagcgcgc gggcgcggcc actcgcagca cgcggggccg
    16981 ccgcacgcaa cccgcgaagc accgccgcgc gcccagcgcg cgggcgcggc cactcgcagc
    17041 acgcgtagtg cagccggacg ccccgcacac agagggcgct cgaacgccca ccacgcgagg
    17101 cacagccgga gatgcatcag acagggcgcg gccattcgtc cagcgtgcgg gaccgccgca
    17161 cacaacaccc ggaggacggc cgcacgccca gcaggcggaa caccgtcgca cgcttaacac
    17221 gcgcacggcc actcgcacac gcgcagaacg gtcggacgcc ccgcacgcag agcgcaggcg
    17281 aatgccccac cacgcgaggc gcagtcggac gtccatcagg caggggcccg cacgcacaac
    17341 acgcgggaca cggtcggtcg ttgagggcgt gggcctcttg gacgcgttgc gcttctgcgt
    17401 ggcctcgtct tcctcaaggt ttgggccgcc atggagaggg cgagggggat gtgcgagggc
    17461 cgggactgcg gtcgctgctg cggccggggc tgccgtggtt gctgtcaggt gtcggtggat
    17521 ggacggatcg gcggcgttcc ttcgtgggat cgccaaccga agggcgccct tacagggttc
    17581 tagccgggtg gatacctgcc atctaccggc ttctcgcagg tactgggcat ctgtgttgcc
    17641 gtcggggcct cgtgagcgcg ctggacgact tggttgggat cccgcggtcc cgccgacctg
    17701 attggcgagc gcttccctgt gtgcgtcctg ctgcacgggg cggtgagccg ctggcgagtt
    17761 ggcgcgcaag cctgggggct tggagttcag ctgtcacatg ggttgcaggc ccccttctcg
    17821 gtcgggtcgg tgccgggctg gtgttcagga cggatcaggg cgatgtggtc cggtgcccgg
    17881 ggtgtggggc gtcgtccagt tatggcgacc ttcctgatca ggccggacga gttgacactg
    17941 tgagattcgg cttggacacc ttcttggtgg gggtggtgac tgagaggttc gggctgccgg
    18001 ggtgggcgta ggcgggggtc agacgaggat cactcgacgt ccgcgtgtgt ggccggagcg
    18061 gctatcggcg tgcgcggcgg cggcctcggg cagcgtgtac gacttctgga cgggaatgtg
    18121 gagctttccg cccgagatga ggcggacggt ctcggcgagc gcgtccctta tgctcccggc
    18181 ctggttggag aaccggacac cgagcttcgg cgcgtcgagg tcggcgatgg agacgacctt
    18241 ccgcggatcc ccggtcagct cgacgagttc actgatcaca cccgcgccgg ccaggtcgag
    18301 ggccgcgtcg gccttgccca gcccccgcac ccgctcgacc cagcccgcgc cgtacgtggt
    18361 ggcgacggcg cccaggccgc gcacgtactc ctggttcgcg gggctggctg tgccgatcac
    18421 cgtgataccg cgttcgcggg cgatctgtag caccgcggat ccgactcccc cggccgcgcc
    18481 gctgaccagc agcgtctggc ccggttgcac accgacctcg cggaggacgc gcagcgcggt
    18541 ctccatcacg gaggggtacc cggccgcctc ctcgaacgtc aggcccgtgg gcatttgggc
    18601 ccaggcgttc agaaccgaga actcggcata ggtgtcggtc ccctcaccga acacgtggtc
    18661 gccgaccgcg accccttcga cgccgtcacc gacctcgtcc accacaccgg cggcgtccag
    18721 gccgaccccg gcggggaggg tgaccgggtg ggcgcccagg acctggcctt ctcggagccg
    18781 ccagtcgacg gggttcacgc ccgccgcccg cacggcgacg cgtacttcgc cggggcccgc
    18841 gtggggctcc tcggcgtcca ggagttgcag aacgtcagga ccgccgaact cggcgtagat
    18901 caccttcttc atgcggtcga cgatagcact aacagttagc tttttgaatc tgatacgatt
    18961 ttgaatctgg tagcatctgg gcatgacggt gccgccggga cgtcgggagc gcaagaaggc
    19021 cgcgacccgc cagaagatcg ccgacgcggc catgcgactc ttcctggagc gcgggtacga
    19081 cgcggtgggc atccgtgacg tggccgccga ggccgacgtg gccgtcacca cgctcttctc
    19141 ccacttcgcc tccaaagagg ccctggtgtt cgagcaggac caggacttcg agcagcgcct
    19201 cacccgggcc gtcaccgacc gggacccgca cgagccgctc atccccgcgc tgcgtcgcga
    19261 gatcgaggcc ctgctgcgac actgcggggc ggacagcgcc cttccgatct ggcagatggt
    19321 cgacgaatcg cccgccctgc ggcagtacga gggggcgatg cggctgcgcc acgcggagtc
    19381 ggtggcgacg gccatagccg gagccctcga tctgccggag tccacgacgt cctgccggac
    19441 gatcgccagg ttcgcgatcg acgccttctc actggcccgc gaggcggccg atccggatgc
    19501 cgcgctgaac gagatcttcc agatgatcga ggcggcctgg gaggtctccg gccttccctg
    19561 gcatgggcga gggaccgata aggccgaggc cggtcaggcc gtcaccagct cctgaatccc
    19621 agggcccacc tgacttgatt ggtaggacgc cgtccccttg gcaaggggcg actccggcgg
    19681 gggcaagcgg caatctgacc tgggcacccg tgtgcagggg ccgtaacgat ctcgtatgcg
    19741 cattacaagg gcaaacttcc ggcgggctgc ctgcgcaggg gcgcccgccg atcacgtcta
    19801 ctcacggctg cctggctgta ggtgagcgtg gctcatgcgg cgcgatacgc acgctgtcgc
    19861 gatagcgcgg gatagcccgt tgggcctgat tgctaagcga tgccgcgctc gggcccgggg
    19921 cgggcttcag gggccgtagc cgccatgcct tcgggacggg acggaggcgg gcgaccgggt
    19981 aggcgtgctg cgcccgcgcc cggccccggc gtcccacacg gtgagccgtt ggatggattc
    20041 cacgtgccct gaaggccagg gaagtggtca aaattcgaga acgttcgtag gtgaaggtca
    20101 cctagcgtca ccggcgttgc tgattcgttg acggaggtcg cgcaggggac aggatcaacg
    20161 ggtggacggg cgcgaaaacg ggagccccga acgcccgggg tgttctcgcg gtgcatgcag
    20221 cgccgaatga acgcccgtat gaatcgcaag gtccgccgtg gtcgtggcac gttcatgggc
    20281 atgtacgtgc tcaacctgca cacgtccggc tgccggagcg ggcggcctcg ggaaacgccg
    20341 ctggcgtggt gcgccgacgg tgacgacgcg cgcctcgtcg tcgcgtcggg cgggggggga
    20401 gtcggcaccc cgactggcat gccaatctga tggcgcatcc gagcagacgg ctaccgattt
    20461 accgggacac gaggccgtgc cggtgacgcc gcacccgctc gacggtgcgg agcgcgagga
    20521 agcgtggcgc cggatcgcgg ccgctcaacc tcggatcgcc aagtaccagg ccgagtcaaa
    20581 acgggtgtac ccggtgattc ggctcgcccc cgcgatgact cgctaatttc ctgcggcggc
    20641 ctcgtccagg agccaggtga catttccacc cgcctgttcg atggtgtggg cggccagtgc
    20701 cactggaagg tcgaggctgc ccgatcgcgc ttcggagacg gcggcggcct tgcccttgcc
    20761 cgccgcgacg atcaggcagt tccgggtggc gcggagcgcg ccgagagtga gggtgatgcg
    20821 ggtcggcggc ggcttggggg aatcgagaac gggaatgacg ctcggggtgg tgatgtcgag
    20881 ttccgggtgg ccggggaaga gggagaggca atggccgtcc tcccccatgc cgagccaaac
    20941 atggtcgagg cgggggccga actcggtttg cccggggagt tcgctcagga ttttcgcgta
    21001 ttcggcggcc gcttcgtccg gcggcaggtg gacggggggc ggcgtccggc cggactcggg
    21061 gatcgggacc ttggagagca gcaggtcggc ggcctgacgc cagttggagt
//
