LOCUS       NZ_JAICDG010000003     10933 bp    DNA     linear   CON 29-AUG-2024
DEFINITION  Actinomadura terrae strain OS3-83 contig3, whole genome shotgun
            sequence.
ACCESSION   NZ_JAICDG010000003
VERSION     NZ_JAICDG010000003.1
KEYWORDS    WGS; RefSeq.
SOURCE      Actinomadura terrae
  ORGANISM  Actinomadura terrae
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Thermomonosporaceae; Actinomadura.
REFERENCE   1  (bases 1 to 1091803)
  AUTHORS   Lee,S.D., Yang,H.L., Kim,I.S., Choe,H. and Kim,J.S.
  TITLE     Actinomadura terrae sp. nov. and Actinomadura litoris OS3-89,
            isolated from rhizosphere soil of cactus
  JOURNAL   Int J Syst Evol Microbiol 73 (1) (2023)
   PUBMED   36748505
REFERENCE   2  (bases 1 to 1091803)
  AUTHORS   Lee,S.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-FEB-2022) Institute of Jeju Microbial Resources, BioPS
            Co., Ltd., Sancheondan Dongil 16, Jeju 63243, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 141x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022751215.1-RS_2024_08_29
            Annotation Date                   :: 08/29/2024 06:12:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,085
            CDSs (total)                      :: 8,002
            Genes (coding)                    :: 7,936
            CDSs (with protein)               :: 7,936
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 13 of 66
            Pseudo Genes (incomplete)         :: 56 of 66
            Pseudo Genes (internal stop)      :: 3 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 21:31:58
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 936807
            Orig. end                         :: 947740
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAICDG010000003.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10933
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="6"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10933
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10933
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5933](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5933)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            178..729
                     /locus_tag="K2F47_RS23985"
     CDS             178..729
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017555986.1"
                     /locus_tag="K2F47_RS23985"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_242907260.1"
                     /transl_table=11
                     /translation="MHAAVGAIAESGPATWSLRELARRAGVSHAAPAHHFGDKTGLLTA
                     VAAEGYTLFADALERAGDDFYDLGLAYVRFAVDHRAHFEVMFRPELYRTDDPEVDAARR
                     RANEILRRGVRSVAPGAATGETGARDQEDDRTAAIAAWCIVHGFAELWLSGAFGGGLGD
                     GLGDDPVAAASPVIRHLFAR"
     gene            complement(762..2468)
                     /locus_tag="K2F47_RS23990"
     CDS             complement(762..2468)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017617106.1"
                     /locus_tag="K2F47_RS23990"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ubiquinol-cytochrome c reductase cytochrome b
                     subunit"
                     /protein_id="WP_242907262.1"
                     /transl_table=11
                     /translation="MAGIRAPGGRAADGRTGGRAGGRGSGGAVDSVSRALDERFGSTGF
                     ARKALRKAFPDQWSFLVGEIAMYSFVIILLTGVYLTLFFKPSMHEVIYDGSYTKLRGVE
                     MSEAYASSLKISFDVRGGLLVRQIHHWATLIFMGAILVHLLRNFFSGAFRKPRELNWII
                     GVTMLVLVMVNGLFGYSLPDDLLSGTGIRILEGVIAAVPLVGSYLVMFVFGGEFPGADI
                     IPRLYVVHVLLIPGLLAALIPLHAVVLTWRQTHTQFPGKGSSNTTVRGYPFFPVFMAKT
                     SALFLWVLGVTTLLAAFVQINPIWLFGPYDPGAISAGSQPDWYMGWLEGSLRIMPAWEI
                     TAWGHTVSMSVVVPSLVVPGLLFTGLGVYPFVERWVTGDHQIHHLLDRPRDVPARTGIG
                     VGGIVFYGLLWLAGGNDVLAHKFSVSLYATTWFFRIAVILGPVLAYIVAYRICVGLQRR
                     DAGLVEHGLETGVIRQSPDGRFSEVERHLPAERIAAITDPRPAPAVDLAVPPPVDGVEN
                     PRGRRLTGRVRAALNRRFRADLATIPERNGHSGQRELGERRELAESESNPG"
     misc_feature    2538..2553
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 16.67"
                     /tool="antismash"
     gene            complement(2575..3228)
                     /locus_tag="K2F47_RS23995"
     CDS             complement(2575..3228)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="K2F47_RS23995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_242907264.1"
                     /transl_table=11
                     /translation="MGAGLDDSARRRFDLTMLHAVHGAFRRDMTVMELAGRLAAGGPFM
                     ADAGPFPVDANAWTLFRFHWLAQRAAERRALWSVLRGRVSGGRSAALEAMDVQAVRLVA
                     LMDRVDAALEHEDDDVLVLLARKLPAALDAHLDFKEAHVLPLIHEALTPYEWGTYEVEF
                     RREIGVRGLRVFLPWLLDGASDRTCAAVLRLLPPPVRLAYRTRWLPRYRRSLRH"
     misc_feature    complement(3315..3329)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 17.88"
                     /tool="antismash"
     gene            3405..4166
                     /locus_tag="K2F47_RS24000"
     CDS             3405..4166
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="K2F47_RS24000"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="DUF4261 domain-containing protein"
                     /protein_id="WP_242907266.1"
                     /transl_table=11
                     /translation="MIDDQTLMSSRHVLCVLGTGLDLDAVEAVAAREGFELDWEYSGPG
                     ADPRMPEAFEACVARASFTRADWDAVDAHDTVAYVLSPPILPGVAFTVARRTLVLTAEL
                     LRSGATAVKNESSGLTHGRDRWLDLAAGVYGGGPEESALPLYRAWVKRPIADGSLLHSC
                     GMHLLGAPDIEIAPLGDPAFAPSPEEVAALIDGLAIYLLTEHRAEEIEDGEGFRLALDA
                     PRWILRHRPCDRYEEDDIFFNPHGYWRLTTE"
     gene            complement(4175..5008)
                     /locus_tag="K2F47_RS24005"
     CDS             complement(4175..5008)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF021364.5"
                     /locus_tag="K2F47_RS24005"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DNA-binding domain-containing protein"
                     /protein_id="WP_242907267.1"
                     /transl_table=11
                     /translation="MGSAPPTLDAAQQWLLHALTVPGASAEQAEAMLAGTGRLGAARRL
                     EIYRLGYRRRLLDTMRHMHPALRELLGPDLFDVFAAGYLDAHPPRAYTLARLGDRFAAY
                     LEEQRPDRGLPVEEREPWADLMVDLVRYERLFAEVYDGPGTEEEPPAPWPTGLPDRVRG
                     APGVRLLRACAPVHAYHSAVRRGARPAPPAFVPARIVVFRRNYRVVTASPPADAFVLLE
                     ALMGGAPPEQASARAGLDPADARRHLRRWLAQRWIVALPALPAPPPREPPGREPP"
     gene            complement(5001..5933)
                     /locus_tag="K2F47_RS24010"
     CDS             complement(5001..5933)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017619267.1"
                     /locus_tag="K2F47_RS24010"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_242907269.1"
                     /sec_met_domain="DUF692 (E-value: 2.1e-96, bitscore: 318.1,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTAESAERAPVPEATAGAAAPDRRCALPGGDASGLGLGLRSSHLS
                     QILAEWPDVGWFEIISENFMDSGGYPRHALGRIAERYPVVMHGVSLSIGSTDPLDFGYL
                     ARLRRLADSTGARWVSDHVCWTGVAGVNTHELLPVPFTERSLRHLARRVEIVQDVLERP
                     LVLENPSTYAAFAHSTMTEWEFLARLCEESGCGLLLDVNNVHVSAFNNGFDPEEYIRAL
                     PHDRVVQIHVAGHADMGDHLIDTHDGPVSEPVWDLYRLATSLAGPVPTLLEWDDRLPPL
                     PALLDELHKARDHLGTPGAGLRDAGAGDG"
     misc_feature    complement(5082..5084)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(5930..6193)
                     /locus_tag="K2F47_RS24015"
     CDS             complement(5930..6193)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="K2F47_RS24015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_242907271.1"
                     /transl_table=11
                     /translation="MNGAIHGGGELHVCMTRNACAGPGGCGYLRLEAEWSVPGSAAWRR
                     ARRVFEQRMVEAGAPLGAGPGLPGGPAPGRDVRDAGATTSGS"
     misc_feature    complement(6245..6260)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 16.24"
                     /tool="antismash"
     gene            complement(6389..7366)
                     /gene="nrfD"
                     /locus_tag="K2F47_RS24020"
     CDS             complement(6389..7366)
                     /codon_start=1
                     /gene="nrfD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017570170.1"
                     /locus_tag="K2F47_RS24020"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="WP_242907273.1"
                     /transl_table=11
                     /translation="MSAAGARKGTRGRRGRRGERAMVPDAEFTSYYGRPVLKAPRWKAP
                     DIAGYFFLGGLAGAGSVLAAGAQLTGRPATARALKVSSLLAISGSAAALVHDLGRPARF
                     HHMLRVLKPTSPMSLGSWLLMAYGPAAGGAAVLDVARLFPRLGAAATAGAAVLGPAVAS
                     YTAVLAADTAVPAWHEAYRELPFVFAGSATAAASGMALLTAPPAETGPARSTAVAGAVL
                     ETAASRLMRRRLGMVAEVYEGGRAGGLTRAAEILTVAGAAGAALLAGRSRTAAALSGAA
                     LLTASACTRFGVFYAGVRSAEDPKYTVVPQRERLNARAEGDSVS"
     gene            complement(7363..8157)
                     /locus_tag="K2F47_RS24025"
     CDS             complement(7363..8157)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017614115.1"
                     /locus_tag="K2F47_RS24025"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="WP_242907550.1"
                     /transl_table=11
                     /translation="MGFFTDTSVCIGCKACEVACKEWNAVPEDGLEFTGMSYDNTQGLG
                     SDTWRHVAFIEQDKPVGNAETGAAEGGLRWLMASDVCKHCTHAACLDVCPTGSLFRTEF
                     GTVVVQQDICNGCGYCVPACPYGVIDQRKEDGRVWKCTLCYDRLGAGQTPACAKACPTE
                     SIQFGPLEELRERAAARVDQLHDQGVDDARLYGENPADGVGGDGAFFLLLDEPEVYGLP
                     PDPVVTTRDLPSMWRHAGAAALTLLGGAAAVFAVHGRRGGRR"
     misc_feature    8628..8641
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.46"
                     /tool="antismash"
     gene            8750..9403
                     /locus_tag="K2F47_RS24030"
     CDS             8750..9403
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017619040.1"
                     /locus_tag="K2F47_RS24030"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF4360 domain-containing protein"
                     /protein_id="WP_242907275.1"
                     /transl_table=11
                     /translation="MRKGIAISAAALAMTATAAVAAPPATASAAYFTRGPNGVTIELAT
                     VNGSGCPLGTAAVAVSEDKEAFTVTYSNYMVQVGGNSNSTDFRKNCQLNMKVHVPQGFT
                     YAILSADYRGFASLEPGATGLEKASYYFQGMPQTGRAEHGLKGEYADNWAFTDVNEVAQ
                     LVYKPCGEERNFNINTELRVNKGTSDPAKVSMMAMDSTDTSIKTTYHFAWKRCP"
     gene            9506..10162
                     /locus_tag="K2F47_RS24035"
     CDS             9506..10162
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017626885.1"
                     /locus_tag="K2F47_RS24035"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF4360 domain-containing protein"
                     /protein_id="WP_242907277.1"
                     /transl_table=11
                     /translation="MRKAMTASAACAAALALAGGLAPSASATTDDPPPSDRIVIDVLTV
                     NGSGCPAGTTAVATASDNTAFTVTYSNYLAQAGGDSRATDFRKNCQINLRVHVPQGFTY
                     AIAGADYRGFAHLEDGASGLERANYYHQGMQQSTPVSHTIKGSFSDDWQFSDRTPVTEL
                     VFKPCGEDRNFNVNTELRVNKGSSDASKTSFMAFDSTDGSVKTTYHFAWKRCPGA"
     misc_feature    complement(10665..10679)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 17.3"
                     /tool="antismash"
     misc_feature    10806..10819
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 18.47"
                     /tool="antismash"
ORIGIN
        1 ggcatggcgg tgtcctgtcg gtcggcgaat cttgtcactg tctagattgc gccatacgat
       61 ccgaacttgt caatgacaag ataggctgcc gggtgacgtc gggggcatcg cgaccacgag
      121 ggggcgagcc ggatcgagca gccgagctat caccacggcg acctgcggcg ggccatcatg
      181 cacgcggcgg tgggggccat cgccgagtcg ggccccgcca cctggagcct gcgcgagctg
      241 gcgcgccgcg cgggcgtctc gcacgcggcg cccgcccacc acttcggcga caagacgggc
      301 ctgctcaccg ccgtcgccgc cgagggctac acgctgttcg cggacgcgct ggagcgggcg
      361 ggcgacgact tctacgacct cggcctcgcc tacgtccgct tcgccgtcga ccaccgggcg
      421 cacttcgagg tgatgttccg gcccgagctg taccggaccg acgaccccga ggtggacgcc
      481 gcccggcggc gcgcgaacga gatcctccgg cgcggcgtcc gcagcgtcgc accgggggcg
      541 gcgaccggcg agaccggcgc gcgcgaccag gaggacgacc ggacggccgc gatcgccgct
      601 tggtgcatcg tgcacggctt cgccgagctg tggctgagcg gcgcgttcgg cggcgggctc
      661 ggcgacggcc tgggcgatga ccccgtggcc gccgccagcc cggtcatccg gcacctgttc
      721 gcccgctgac cgccccgccc gccccgctca gccggcgctg ctcagccggg gttgctctcg
      781 gactccgcca gttcgcgccg ctcgccgagc tcccgctgcc cggagtgccc gttccgctcg
      841 gggatggtgg cgaggtccgc gcggaagcgg cggttgagcg cggcgcgcac ccgtcccgtg
      901 agacggcggc cgcgcgggtt ctcgacgccg tccaccggcg gcgggacggc caggtcgacg
      961 gcgggtgccg gccgcgggtc ggtgatcgcc gcgatccgct cggccggcag gtgccgctcc
     1021 acttcgctga accgcccgtc gggcgactgc cggatgacgc ccgtctccag cccgtgctcg
     1081 acgaggccgg cgtcgcggcg ctggagcccg acgcagatcc ggtaggcgac gatgtaggcc
     1141 agcaccggtc cgaggatgac cgcgatgcgg aagaaccacg tcgtcgcgta cagcgagacg
     1201 ctgaacttgt gcgcgaggac gtcgttgccg cccgccagcc acagcaatcc gtagaagacg
     1261 atgccgccga cgccgatgcc cgtccgggcg gggacgtcgc ggggccggtc gagcaggtgg
     1321 tggatctggt ggtcgccggt cacccagcgt tcgacgaacg ggtagacgcc gaggccggtg
     1381 aacagcagcc cggggacgac cagcgagggg acgaccacgc tcatcgacac cgtgtggccc
     1441 cacgccgtga tctcccacgc cggcatgatc cgcagcgagc cctccagcca gcccatgtac
     1501 cagtcgggct gcgagccggc gctgatcgcg cccgggtcgt aggggccgaa cagccagatc
     1561 gggttgatct ggacgaacgc cgccagcagc gtcgtcacgc cgaggaccca caggaacagc
     1621 gccgaggtct tggccatgaa caccgggaag aacgggtagc cccggacggt cgtgttcgag
     1681 ctgcccttgc cggggaactg cgtgtgcgtc tgccgccagg tcagcacgac cgcgtgcagc
     1741 gggatcagcg ccgccagcag ccccgggatc agcaggacgt ggacgacgta cagccgcggg
     1801 atgatgtccg ccccggggaa ctcgccgccg aacacgaaca tcaccaggta ggagccgacc
     1861 agcggcaccg ccgcgatgac cccttcgagg atgcggatgc cggtgcccga cagcaggtcg
     1921 tcgggcagcg agtagccgaa cagcccgttc accatgacga gcacgagcat ggtgacgccg
     1981 atgatccagt tcagctcgcg gggcttgcgg aacgcgccgc tgaagaagtt gcgcagcagg
     2041 tggacgagga tcgcccccat gaagatcagc gtcgcccagt ggtggatctg ccggaccagc
     2101 agcccgcccc gcacatcgaa gctgatcttc agggacgagg cgtaggcctc gctcatctcg
     2161 acgccgcgca gtttggtgta ggagccgtcg tagatgacct cgtgcatgct cggcttgaag
     2221 aacagcgtga ggtacacccc ggtcaacagg atgatcacga acgagtacat ggcgatctcg
     2281 ccgaccagga agctccactg gtccgggaac gccttgcgca gcgccttgcg ggcgaagccg
     2341 gtgctgccga agcgctcgtc cagcgcccgg gacaccgagt ccacggcccc gcccgaaccg
     2401 cgcccgcccg cgcggccgcc cgtgcgcccg tccgccgccc ggcctccggg cgcccgaatc
     2461 cccgccatcg tcacagtcct ttcgtcgagc ctctggccct aagacagatc tcgacccgcg
     2521 aacgtgacat gtagaggtgt gcccctcgtc acagggacgc gcgtccgtgt gacgtcagtg
     2581 gcggagggag cgccggtagc gggggagcca gcgcgtccgg taggcgagcc tgaccggcgg
     2641 cggcagcagc cgcagcaccg cggcgcacgt ccggtcggac gcgccgtcca gcagccacgg
     2701 caggaacacc cgcagccccc gcacgccgat ctcccggcgg aactccacct cgtaggtgcc
     2761 ccactcgtac ggggtgaggg cctcgtggat cagcggcagg acgtgcgcct ccttgaagtc
     2821 cagatgcgcg tccagcgccg ccggaagctt ccgcgccagc aggacgagga cgtcgtcgtc
     2881 ctcgtgctcc agcgcggcgt cgacccggtc catcagtgcg acgagccgga cggcctgcac
     2941 gtccatcgcc tccagcgccg ccgaccggcc gccgctgacc cggccgcgca gcacgctcca
     3001 cagcgcgcgc cgctccgccg cccgctgcgc gagccagtgg aaccggaaca gggtccaggc
     3061 gttcgcgtcg accgggaacg gtcccgcgtc ggccatgaac ggcccgccgg ccgccagccg
     3121 tcccgcgagc tccatcaccg tcatgtcgcg ccggaacgcg ccgtgcacgg cgtgcagcat
     3181 cgtcagatcg aaacgccgcc gcgcgctgtc gtccaacccc gcacccatcc ctcgcacccc
     3241 gcccctcggc acacccggca accgacctct ccccaggaga tgggcgggac ctctcgaatg
     3301 tgacatgtaa gggcatcacc agcggtgata cgggtctcac cggaacgctc cggttcggcc
     3361 gaaatcgctg cccggggcac ggcccgtcgc gtaacctgcg ccgcgtgatc gacgaccaga
     3421 ccctgatgag ctcccgtcat gtgctgtgcg tcctcgggac cgggctcgac ctcgacgcgg
     3481 tggaggccgt ggcggcgcgg gagggcttcg agctcgactg ggagtactcc gggcccggcg
     3541 ccgacccgcg gatgccggag gcgttcgagg cgtgcgtggc gcgggcgtcc ttcacccggg
     3601 ccgactggga cgccgtcgac gcgcacgaca cggtcgccta cgtgctgtcg ccgccgatcc
     3661 tgccgggcgt cgcgttcacg gtcgcgcggc ggaccctcgt gctgaccgcc gagctgctgc
     3721 gctccggcgc gaccgccgtg aagaacgaga gcagcggcct cacccacggc cgggaccggt
     3781 ggctcgacct cgccgcgggc gtctatgggg gcgggccgga ggagtcggcg ctgccgctgt
     3841 accgggcgtg ggtgaagcgc ccgatcgccg acgggtcgct gctgcacagc tgcggcatgc
     3901 acctgctcgg cgcgcccgac atcgagatcg cgccgctcgg cgacccggcc ttcgccccgt
     3961 ccccggagga ggtcgcggcc ctcatcgacg ggctcgccat ctacctgctc accgagcatc
     4021 gcgccgagga gatcgaggac ggcgagggct tccggctcgc gctggacgcg ccccgctgga
     4081 tcctgcggca ccgcccctgc gaccggtacg aggaagacga catcttcttc aacccgcacg
     4141 gctactggcg cctcaccacc gagtagccgc agggctacgg cggctcccgc ccgggcggct
     4201 cccgcggcgg cggcgccggg agcgccggga gcgcgacgat ccagcgctgc gcgagccagc
     4261 ggcgcaggtg gcggcgggcg tcggcggggt cgagcccggc gcgcgccgac gcctgttccg
     4321 gcggcgcgcc gcccatcagc gcctccagca gcacgaacgc gtccgccggc ggggaggcgg
     4381 tgaccacccg gtagttgcgc cggaacacca cgatccgggc cgggacgaac gcgggcggcg
     4441 ccgggcgggc gccgcgccgg acggccgagt ggtacgcgtg caccggagcg cacgcccgca
     4501 gcagccgcac gccgggcgcg ccccgcaccc ggtcgggcag ccccgtgggc cagggggcgg
     4561 gcggctcctc ctcggtgccc gggccgtcgt acacctcggc gaacagccgc tcgtagcgga
     4621 cgaggtcgac catcaggtcg gcccacggct cccgctcctc gaccgggagc ccgcggtcgg
     4681 gccgctgctc ctccaggtac gcggcgaacc ggtcgccgag ccgggccagt gtgtaggcgc
     4741 gcggcgggtg cgcgtcgagg tacccggcgg cgaacacgtc gaacaggtcg gggccgagca
     4801 gctcgcgcag cgcgggatgc atgtgccgca tggtgtccag caggcggcgc cggtagccga
     4861 ggcggtagat ctccagccgc cgcgccgcgc cgaggcggcc ggtgcccgcg agcatggcct
     4921 cggcctgctc cgccgacgcg cccggcacgg tgagggcgtg cagcagccac tgctgcgccg
     4981 cgtccagcgt cgggggcgcg ctacccatcg ccggcccccg cgtcgcgcag cccggcaccg
     5041 ggcgtgccca ggtggtcgcg ggccttgtgc agctcgtcca gtaacgcggg gaggggcggg
     5101 aggcggtcgt cccactccag gagggtcggc acggggccgg ccagcgacgt ggccagccgg
     5161 tacaggtccc agaccggttc ggagaccggg ccgtcatggg tgtcgatcag gtggtcgccc
     5221 atgtcggcgt gcccggccac atggatctgg acgacccggt cgtgggggag cgcgcggatg
     5281 tactcctcgg ggtcgaagcc gttgttgaac gccgagacgt ggacgttgtt gacgtccagc
     5341 agcagcccgc accccgactc ctcgcacagc cgcgcgagga actcccactc ggtcatcgtc
     5401 gagtgcgcga acgcggcgta ggtgctcggg ttctccagca cgagcggccg ctccagcacg
     5461 tcctgcacga tctcgacgcg ccgcgcgagg tgccgcagcg accgctcggt gaacgggacg
     5521 ggcagcagct cgtgggtgtt gacgcccgcg acgcccgtcc agcacacgtg gtcggacacc
     5581 caccgcgcgc cggtggagtc ggccagccgg cgcagccggg cgaggtagcc gaagtcgagc
     5641 gggtcggtgc tgccgatcga cagcgacacc ccgtgcatga cgaccgggta ccgctcggcg
     5701 atgcggccga gcgcgtgccg cgggtagccg cccgagtcca tgaagttctc ggagatgatc
     5761 tcgaaccagc cgacgtccgg ccactcggcg aggatctggc tcaggtgcga ggagcgcagc
     5821 cccagcccga gcccggacgc gtcgccgccc ggcagggcgc accgccggtc gggcgcggcg
     5881 gcaccggccg tggcctcggg cacgggcgcc cgctccgcgg attccgccgt catgacccgg
     5941 aggtcgtcgc gccggcgtcg cgcacgtcgc ggcccggggc gggccctccg gggagcccgg
     6001 ggcccgcacc gagcggcgcg cccgcctcga ccatccgctg ctcgaagacc ctgcgcgccc
     6061 gccgccacgc ggcgctgccg gggacggacc actcggcctc aagccgcagg tagccgcagc
     6121 cgcccggccc cgcacaggcg ttccgcgtca tgcacacgtg cagctcgccg ccgccgtgga
     6181 tggctccgtt cacggggtgt gcctccctcg tcgagatcat gtccgggatg acgtctcgca
     6241 acaggctaat cacactcagt agtcaacggc gcgacagatt tatgtcacct gtccccgagc
     6301 ggagtgtgcc gcgatgggga tggcctggta aaagaccggg ccggtcggct ttctccgggc
     6361 cgaggggagg ggagatagag gtgcgccgtt atgacacgct gtcgccctcg gcgcgggcgt
     6421 tcaggcgttc gcgctgcggg accaccgtgt acttcgggtc ctccgccgag cgcacgcccg
     6481 cgtagaagac gccgaagcgg gtgcacgcgg acgcggtgag cagcgccgcc ccgctgaggg
     6541 cggccgccgt ccggctccgt ccggcgagca gcgcggcccc ggcggcgccc gcgacggtca
     6601 ggatctcggc cgcgcgcgtc aggccgcccg cgcggccccc ctcgtacacc tcggccacca
     6661 tgccgagccg ccgccgcatc agcctggacg cggcggtctc cagcaccgcg ccggcgaccg
     6721 ccgtgctgcg cgccggtccc gtctccgcag gcggtgcggt gagcaacgcc atcccggacg
     6781 cggcggccgt cgccgaaccg gcgaacacga aaggcagctc gcggtacgcc tcatgccagg
     6841 cgggaacagc cgtgtcggcc gcgagcacgg cggtgtagga cgcgacggcg ggcccgagca
     6901 cggccgcgcc ggccgtggcc gccgcgccga gccgggggaa caggcgcgcc acgtccagga
     6961 cggcggcgcc gcccgccgcc gggccgtagg ccatcagcag ccacgagccg aggctcatcg
     7021 gggacgtcgg cttgaggacg cgcagcatgt ggtggaaccg ggcgggccgt ccgaggtcgt
     7081 ggaccagcgc ggcggccgac ccgctgatcg ccagcagcga ggagaccttc aacgcgcggg
     7141 cggtggcggg acgcccggtg agctgcgcgc ccgccgcgag caccgagccc gcgcccgcga
     7201 gcccgccgag gaagaagtac ccggcgatgt ccggcgcctt ccacctcggc gccttgagga
     7261 cgggtctgcc gtagtaagag gtgaactccg cgtcgggcac catcgcccgc tcgccacgcc
     7321 gcccccggcg tccgcgggtg cccttccgtg ccccggccgc gctcaccggc ggccgccccg
     7381 gcgcccgtgg acggcgaaga ccgcggcggc gccgccgagc agggtcagcg cggcggcgcc
     7441 cgcgtgccgc cacatcgacg gcaggtcgcg ggtcgtcacg acggggtcgg ggggcagccc
     7501 gtacacctcc ggctcgtcca gcaggaggaa gaacgcgccg tcgccgccga ccccgtccgc
     7561 cgggttttcg ccgtagaggc gcgcgtcgtc cacgccctgg tcgtggagct ggtccacgcg
     7621 cgcggcggcc cgctcccgaa gctcctccag cgggccgaac tggatcgact cggtggggca
     7681 cgccttggcg cacgcgggcg tctgccccgc cccgagccgg tcgtagcaca gcgtgcactt
     7741 ccagacgcgc ccgtcctcct tgcgctggtc gatgaccccg tacgggcacg cgggcacgca
     7801 gtagccgcac ccgttgcaga tgtcctgctg gacgacgacc gtgccgaact ccgtgcggaa
     7861 cagcgacccg gtggggcaca cgtccaggca ggcggcgtgc gtgcagtgct tgcagacgtc
     7921 ggaggccatg agccagcgga gcccgccctc ggcggcgccc gtctcggcgt tcccgaccgg
     7981 cttgtcctgc tcgatgaacg cgacgtgccg ccaggtgtcc gagccgagcc cctgggtgtt
     8041 gtcgtaggac atccctgtga actccaaccc gtcctcgggg acggcgttcc actccttgca
     8101 ggcgacctcg cacgccttac acccgatgca gaccgaggtg tcggtgaaga agcccatgcg
     8161 cgggggcgcg tcggcgtacc cggcgtcggc ggcgacgccg ggctcggctc cggcgagcag
     8221 gttgcgtccg atcaggctgc tcatccccac gacctccaac gacgcccagg ccaaggcacc
     8281 cttcccctcg tcgcgcgccc tacacctcgg gtcgccgcgg ccccgtgccg ctttccaggc
     8341 gttcgaccgg cgggggcccg cgatttcggg cctacgcccc gcccgcctgc atgggccgcc
     8401 gggcgacccg gcggccggcc cgcgaccccg cgtcccgcgc caggcggggc cactcttcta
     8461 gcgactctga acggttcctg aaattcgatt gaccctgttt ctggaacggt ccccctgcaa
     8521 tggggggtgc cggttgaatg ttggacagtc cgttttcgca gattggacgg gttttcgccc
     8581 ggttacggaa tgagattacg cgttgccgta ccccgacatt caagggaagt taaaaagaag
     8641 acgttgaccg gcttgatcgg cgccgtctac tctctcgggc agatcctgga tcacgttcca
     8701 actcttcggc cgtagaaacc gcgtccgccc ccagcaaaga ggaaatcgca tgcgtaaagg
     8761 gatcgcaatt tccgctgccg ccctggcgat gaccgccacg gccgccgtcg ccgccccacc
     8821 ggcaacggca tccgccgcct acttcacccg cggcccgaac ggcgtcacca tcgaactcgc
     8881 gacggtgaac ggctcgggtt gcccgctcgg aaccgcggcc gtggccgtgt ccgaggacaa
     8941 ggaggcgttc accgtcacct acagcaacta catggtgcag gtgggcggca actccaactc
     9001 gaccgacttc cgcaagaact gccagctcaa catgaaggtg cacgtcccgc agggcttcac
     9061 ctacgcgatc ctgtcggccg actaccgcgg cttcgcctcg ctcgagcccg gcgccaccgg
     9121 actggagaag gccagctact acttccaggg catgccccag accggtcgtg cggagcacgg
     9181 cctgaagggc gagtacgccg acaactgggc gttcaccgat gtgaacgaag tcgcgcagct
     9241 ggtgtacaag ccgtgtggcg aggagcgcaa cttcaacatc aacaccgagc tgcgcgtcaa
     9301 caagggaacc tcggacccgg ccaaggtcag catgatggcc atggactcga ccgacaccag
     9361 catcaagacg acctaccact tcgcctggaa gcgctgcccc tagtccagcg ctcctttcac
     9421 atcgcgactg ccgggaattg cgtctagaac ggcagcagcg tgtccccgag aaacactctc
     9481 cacccgagtt aggagaagag cacccatgcg caaggcaatg accgcttcgg cggcgtgcgc
     9541 cgccgcactg gccctggccg gcggtttggc tccgtccgcg tccgccacca cggacgaccc
     9601 gcccccgtcg gaccggatcg tcatcgatgt ccttaccgtg aacggctccg gctgccccgc
     9661 gggcaccacc gccgtcgcca cggcgagcga caacaccgcg ttcaccgtca cctacagcaa
     9721 ctacctcgcc caggcgggcg gggactcgag ggcgacggac ttcaggaaga actgccagat
     9781 caacctgcgg gtgcacgtgc cgcagggctt cacctacgcg atcgccggtg ccgactaccg
     9841 cggcttcgca cacctggagg acggcgcgtc cgggctggag cgcgcgaact actaccacca
     9901 gggcatgcag cagtcgaccc cggtcagcca cacgatcaag ggttcgttct cggacgactg
     9961 gcagttctcc gaccgcacgc cggtcaccga actggtcttc aagccctgcg gtgaggaccg
    10021 caacttcaat gtcaacaccg aactgcgcgt gaacaagggc agctcggacg cgtccaagac
    10081 gagcttcatg gccttcgact ccaccgacgg cagcgtcaag acgacgtacc acttcgcctg
    10141 gaagcgctgc cccggcgcct agctgacacg agccggcccc cgctcacggc catagaggat
    10201 cagggccgcg tccttactcg gggaagggcg cggccctact catgcccgca cgcggcctgt
    10261 cgaggtggcg acagatggcc agccgctgga tccggttcgt accccaagcc ataatcaagg
    10321 cggcccgtca gcggccggtc acgatcggca gggccgaacg cacgtccttc ctgctgtgcg
    10381 tcgaggcgcc gatcggtgcc gaggaggccg atgtggcaag gcgaaggcgg aacctgcgcg
    10441 tcccggcggc ggccctggcc gccggcatcc tcgtcctgac gccgcccgtg gtcacggtcg
    10501 gcgcggcgac aggcaacggc gccgcgctcg gtgacacggc cgaccgggcg acaccgtccg
    10561 acgtcgcccg atgggtggtg accatggacg ccaccggatt acgcggactg ctggcgcgcg
    10621 gccgggtcac gtcggtggcc ctcgtcaggg cctaccaggc gcgcatcgac gcctatgagg
    10681 aggcgtacgc cgaccagccc ggcgtcaacg cggtcatccg ccgggacccc acggcgatcg
    10741 ccgacgccgc ccggctcgac gccgaacgcc ggcgggggca cgtccgcggc ccgctgcacg
    10801 gcatcccgat cctgatcaag gacaactacg acaccggtga cctgcccacc accagcggct
    10861 cgctggcgct gcggcacggg cgtcccgccg acgacgccga gcaggtcgca cggctgcggg
    10921 cggccggcgc gat
//
