LOCUS       NZ_JALDPQ010000063     10945 bp    DNA     linear   CON 28-MAY-2024
DEFINITION  Streptomyces sp. TRM75563 734, whole genome shotgun sequence.
ACCESSION   NZ_JALDPQ010000063
VERSION     NZ_JALDPQ010000063.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. TRM75563
  ORGANISM  Streptomyces sp. TRM75563
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 440662)
  AUTHORS   Chen,Y.
  TITLE     Streptomyces aheensis sp. nov., isolated from road
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 440662)
  AUTHORS   Chen,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2022) College of Life Science, Tarim University,
            Hongqiao, Alar 843300, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 14-MAR-2022
            Assembly Method                   :: ABySS v. V2.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 400.0x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022748615.1-RS_2024_05_28
            Annotation Date                   :: 05/28/2024 05:18:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,476
            CDSs (total)                      :: 7,413
            Genes (coding)                    :: 7,165
            CDSs (with protein)               :: 7,165
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 56
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 249
            CDSs (without protein)            :: 248
            Pseudo Genes (ambiguous residues) :: 1 of 249
            Pseudo Genes (frameshifted)       :: 108 of 249
            Pseudo Genes (incomplete)         :: 202 of 249
            Pseudo Genes (internal stop)      :: 19 of 249
            Pseudo Genes (multiple problems)  :: 75 of 249
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 19:22:52
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 361520
            Orig. end                         :: 372465
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JALDPQ010000063.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10945
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="4"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10945
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10945
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5945](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5945
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            complement(1099..1773)
                     /locus_tag="MQC87_RS21270"
                     /old_locus_tag="MQC87_21270"
     CDS             complement(1099..1773)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1008: response
                     regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003971207.1"
                     /locus_tag="MQC87_RS21270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MQC87_21270"
                     /product="response regulator transcription factor"
                     /protein_id="WP_242740912.1"
                     /transl_table=11
                     /translation="MRVLLVEDDDDLREVIASGLRDGGFAVDCAADWPEADVLLYVTAY
                     DCVVLDRMVPSGDTLVPLEVRRRAGWSVPVLCLTALDSLPERLRGLESGADDYLAKPFA
                     MPELVLRVRGLSRRAAARLPSFLSCADVVMDVARYEVRRGGVLLSLSPKEYAVLQQLLV
                     HRDAVVTRTDLLEHCWDEMADPVSNVVDAVVAGLRRKLGSPGLVHTVRGQGFLLSAEPG
                     PS"
     gene            complement(1856..2446)
                     /locus_tag="MQC87_RS21275"
                     /old_locus_tag="MQC87_21275"
     CDS             complement(1856..2446)
                     /GO_function="GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1042: O-methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007459230.1"
                     /locus_tag="MQC87_RS21275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MQC87_21275"
                     /product="methyltransferase"
                     /protein_id="WP_242740913.1"
                     /transl_table=11
                     /translation="MTDSQGQETGNGGEAAVDRQARGTVARLVLGQMATQARGTAVRFG
                     VFDRIGPGELSGDALAGSLGTHPQATLRLLRALAGLQLLSETVSAISRSMLKSIIRDWD
                     DERCAAILRHIRAVIPDHGSLLIVEPVLPATVPPDPAGGVYLSDLDMLVDVGGRERTED
                     DFAALCAAGGFALRSVTPMPAPDIFRIIEASPV"
     misc_feature    complement(2468..2486)
                     /note="TFBS match to DmdR1, Iron(II)-dependent repressor,
                     confidence: weak, score: 23.4"
                     /tool="antismash"
     gene            complement(2545..3465)
                     /locus_tag="MQC87_RS21280"
                     /old_locus_tag="MQC87_21280"
     CDS             complement(2545..3465)
                     /GO_component="GO:0005829 - cytosol [Evidence IEA]"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1031: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003971209.1"
                     /locus_tag="MQC87_RS21280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MQC87_21280"
                     /product="LysR family transcriptional regulator ArgP"
                     /protein_id="WP_103512265.1"
                     /transl_table=11
                     /translation="MMSELPLDQVRTLLAVVDEGTFDAAAGALQLTPSAVSQRVKALEQ
                     RTGRVLLMRTKPVRPTESGEVVVRFARQLARLERDARTALGMAGPDAPTPLSIAVNSDS
                     LATWFLPALRRVPEQLDLCYELRREDQDHTAALLREGLVMAAVTSSPEAVTGCSVRRLG
                     RMRYLPVASPGFADRWLERRAGTALRELIGDAPVIGFDRRDDLQDTFVRRLGLGPRASA
                     RRHLVPTSEGFVNAVTAGMGWGMVPEVQAEPLLGDGRLVRLTSDRTVVVPLYWQQWKLD
                     SPALAAVADAVAAEAAEALDGDRPD"
     misc_feature    3496..3510
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 17.15"
                     /tool="antismash"
     gene            3533..4144
                     /locus_tag="MQC87_RS21285"
                     /old_locus_tag="MQC87_21285"
     CDS             3533..4144
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_process="GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1116:
                     Homoserine/threonine efflux protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007459226.1"
                     /locus_tag="MQC87_RS21285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MQC87_21285"
                     /product="LysE family transporter"
                     /protein_id="WP_242740914.1"
                     /transl_table=11
                     /translation="MMNALTAAAAGFGTGLSLIVAIGAQNALVLRQGVRRDGVLAVVGI
                     CALSDAVLIALGVAGVGAVVVAWPSALTAVGLLGGLFLIGYGLLAARRVLRPSALHAAD
                     APATSRRGVVLTCLAMTWLNPHVYLDTVFLLGSVAADRGPLRWTFGLGAVLASLCWFVA
                     LGYGARMLSGVLARPAAWRVLDALVAVMMIGLGAKLALDA"
     gene            complement(4206..4844)
                     /locus_tag="MQC87_RS21290"
                     /old_locus_tag="MQC87_21290"
     CDS             complement(4206..4844)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010060641.1"
                     /locus_tag="MQC87_RS21290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MQC87_21290"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_242740915.1"
                     /transl_table=11
                     /translation="MAKQDRAIRTRQTILMAAAQAFEKYGYQAATITEILKSSGVTKGA
                     LYFHFPSKEDLALGVLDAQEPPQTVPDQALKLQELIDLGMLFSHRLQTSLLARAGVRLS
                     MDQQAQGLDRSGPFLRWQEATHHLLSSAKHNGELLPHVNPTETADLYVAAFAGTQAVSQ
                     TLTNYQDLQRRHITLQQHILPSIATPSILTALDLTPERAERLGLLDPVG"
     gene            5001..5945
                     /locus_tag="MQC87_RS21295"
                     /old_locus_tag="MQC87_21295"
     CDS             5001..5945
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003971211.1"
                     /locus_tag="MQC87_RS21295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MQC87_21295"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_242740916.1"
                     /sec_met_domain="AfsA (E-value: 1.1e-26, bitscore: 87.4,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPEAAVLIDPVPAMDSEAEVVHPVGIDMVHRSRPEDAFPRNWVRL
                     ASDRFSVAAVLPHDHPFFAPVSGDRHDPLLVAEAMRQAAMLSFHAGYGVPVGYHFLMAD
                     LDYACDLDHLGVGGAPAEIDVEVFCSDLKWRGGLPVQGRVGWAVHRDGELAATGTGATR
                     FTNPKVYRRMRGDTPAEGIAIPQATPVSTAGAGRTRAEDVVLSETGRDGVWELRVDTRH
                     PTLFQRPNDHVPGMLLLEAARQAACLAAGPGGIVPAEARSRFHRYAEFDSPCSIGAVVQ
                     PGTAEDTVTVQVAGHQGGEPVFSTVLSGPRVNG"
     gene            5958..6803
                     /locus_tag="MQC87_RS21300"
                     /old_locus_tag="MQC87_21300"
     CDS             5958..6803
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1199: NmrA family
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012382293.1"
                     /locus_tag="MQC87_RS21300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MQC87_21300"
                     /product="NAD(P)H-binding protein"
                     /protein_id="WP_242740917.1"
                     /transl_table=11
                     /translation="MILVTGATGAVGREVAGQLAAAGPVRILARSPERLTVRGSGVEVV
                     EGEYGDRSALDRAMRGVTSVFLVTKSPTEPHDEWVAAAAAAAGVRHLVKLSMMAVEEPG
                     ADDFITRRQRENEQAVRESGVAWTFVRPRTFMSNTLSWAPGIRSAGVVRALYGDAPVAC
                     VDPRDVAAVAVAALTGTGHEGRAYAVSGPEAITAREQTAELSRVLERPLRFEELGVDAA
                     RTALLASYPRPVAEAFLESAERQRAGAKATVVPTVEELTGRPARPFRIWSADYADAFAV
                     "
     misc_feature    complement(6818..6833)
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 20.24"
                     /tool="antismash"
     gene            7084..7806
                     /locus_tag="MQC87_RS21305"
                     /old_locus_tag="MQC87_21305"
     CDS             7084..7806
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010985133.1"
                     /locus_tag="MQC87_RS21305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MQC87_21305"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_242740918.1"
                     /transl_table=11
                     /translation="MTKQERATRTRRSLIRSAATVFEQHGYAQARLTLISSGAGVSTGA
                     LHFHFENKAAVAEAVVVEASRELREMSGAIRRRTDTALQALVDSSHALAERLREDPVSR
                     AGFRLSCDAARTAAPDLRVQWHHRVRELLDEAAADGTLAEDVSREDAAAATVAATAGFE
                     VLGRHDSGWLSPYMLTGFWQLLLPRLAAAQTLPLLDPAGTVATASTRIPAPAACATDEA
                     EAAAGVAPEEVAAVAEAD"
     gene            7863..8606
                     /locus_tag="MQC87_RS21310"
                     /old_locus_tag="MQC87_21310"
     CDS             7863..8606
                     /EC_number="2.1.1.-"
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089: methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007459221.1"
                     /locus_tag="MQC87_RS21310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MQC87_21310"
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="WP_242740919.1"
                     /transl_table=11
                     /translation="MADEMFADPRLAALYDPLDPDRGDLDPYVRIAEEFGARRVLDIGC
                     GTGVFALLLADRGVEVVGVDPARASVDVARAKPGGDRVRWISGDASALPPLRVDLATMT
                     ANVAQEITDPRDWRATLRGAYEALRPGGHLVFETRDPARRAWEAWNRERSHRVTEIIGL
                     GPVESWVEVTGVAGPLVSFRWTYVFAVDGRVLTSDSTLRFRERQEVEADLTARGFVVRE
                     VRDAPDRPGREFVLIARRPDPDPRR"
     gene            complement(8569..9498)
                     /locus_tag="MQC87_RS21315"
                     /old_locus_tag="MQC87_21315"
     CDS             complement(8569..9498)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007459219.1"
                     /locus_tag="MQC87_RS21315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MQC87_21315"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_242740920.1"
                     /transl_table=11
                     /translation="MDDQPEAAAPEPLDRRAELSEFLRTRRAKLQPQDVGLPEFGRARR
                     VPGLRREELAQLAGVSVAYYTRLEQGNGRNVSAEVLDAIARALRLTDAEHAHLNHLARP
                     ARHKKRRRPAKVQRVRTGLLYLLDNMEGIPAYVAGARSDILAWNPMAAAVFGDWAALPQ
                     AERNWARLVFLSPAYRDLFLNWESKASDMVSYLRLYAGCHPDDPQLSALVGELSLKSEE
                     FRRLWATHNVKEKGHGIKLIRHPLVGDLTLSYETLNLPDDEGQQLVTYHAEPGSESAQA
                     LRLLASWGADAGRADVISGAGRGPDDER"
     gene            9661..10701
                     /locus_tag="MQC87_RS21320"
                     /old_locus_tag="MQC87_21320"
     CDS             9661..10701
                     /EC_number="1.1.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_ER (19-269). E-value: 5.5e-13.
                     Score: 40.7. Matches aSDomain:
                     nrpspksdomains_MQC87_RS21320_PKS_ER.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_zinc_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1040: alcohol dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019761872.1"
                     /locus_tag="MQC87_RS21320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MQC87_21320"
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="WP_242740921.1"
                     /sec_met_domain="ADH_N (E-value: 3.4e-29, bitscore: 95.6,
                     seeds: 66, tool: rule-based-clusters)"
                     /sec_met_domain="ADH_zinc_N (E-value: 1.9e-24, bitscore:
                     80.8, seeds: 86, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTVAAYAAPRAKAPLERTTIERRPVGEFDILIDIKFAGICHSDI
                     HQARDGWGEGIFPMVPGHEIAGIVAETGSGVTRFKVGDRVGVGCMVDSCGKCDACLMGR
                     EQHCAEGNTQTYNALDRSGEPTYGGYSTHIVVTEKFAVTIPEGIALDEAAPLLCAGITT
                     YSPLKRWGAGPGKKVAVVGLGGLGHMAVKIAHALGAEVTVLSQSLRKKDDGLKLGADHY
                     YATSDPETFEELAGTFDVILSTVSAPLDFGAYLGLLRTDGTLVNVGAPEEPISLNLFSL
                     IMGNRSIAGSAIGGIAETQEMLDFCAVHGLGAEIEVIGASQVNEAYERVLASDVRYRFV
                     IDTATV"
     aSDomain        9718..10467
                     /aSDomain="PKS_ER"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MQC87_RS21320_PKS_ER.1"
                     /evalue="5.50E-13"
                     /label="MQC87_RS21320_PKS_ER.1"
                     /locus_tag="MQC87_RS21320"
                     /protein_end="269"
                     /protein_start="19"
                     /score="40.7"
                     /tool="antismash"
                     /translation="TIERRPVGEFDILIDIKFAGICHSDIHQARDGWGEGIFPMVPGHE
                     IAGIVAETGSGVTRFKVGDRVGVGCMVDSCGKCDACLMGREQHCAEGNTQTYNALDRSG
                     EPTYGGYSTHIVVTEKFAVTIPEGIALDEAAPLLCAGITTYSPLKRWGAGPGKKVAVVG
                     LGGLGHMAVKIAHALGAEVTVLSQSLRKKDDGLKLGADHYYATSDPETFEELAGTFDVI
                     LSTVSAPLDFGAYLGLLRTDGTLVNVGA"
ORIGIN
        1 ggtcggcgac ccacacctcc ggaccgcgca cggtcaccag cagatcggcc cggtccgtcg
       61 gcgcaccggg cgcccggccg tgccgtacgg cgttgtccag gaggttgcgc acagcgaccc
      121 gcaccagggc cgcgtcggct tccgtcaccg actccgtcag ccgcagctcc gggccgcgac
      181 cgctgtccgg cagctccgcg cacacctcct cgacgagctg gtccagccgc agcggctccg
      241 cccgtacgac gtccacgtcg gcctccaacc gcccccgggt cagcaggttc tccacgacgt
      301 cggccatccg gtccgccgcc cgccggatcc ggggcagatg cgccgccggc ccgtccgggg
      361 aggcggcggc caaatccacc gatccgcgca tcaccgcgac cggggtccgc aactcgtgcg
      421 ccgcgtcggc cagcagccgt tcctgctgct ccagcgtctg ccaggcaggc cgcatgctcc
      481 gcccggagag cacctgcccg gtcagcgcgg ccagcgccgt cagcgccccg gtcccgacga
      541 cgagggagag caccagactg cggtgctcgg ccgcgccgta ctccggatcg ccgaccgcga
      601 cggccgcgcc cgccacctcg tcggtggcgt cgtggtagaa gggcacggcc agcagatgca
      661 ccggctctcc cgtacggtcg cgcgcctcgg cccgtaccgg ttcctcctcg tccatcgccg
      721 tacgggtcac cgcccacaga tctccggccg gtacggcgaa ccgctcggtg cggccccgga
      781 acacggtcac cggatcaccg ccgggccgtc gctccagcac caggatctgc ggggtgcccg
      841 aggtcgcgtc gtcgtcgtcg aggccgtcca gccggatgcg gtcgtcctcg tagtacacga
      901 gcgaggccgc gacggacgcc cgtcggcgca tctcgtcgta ctcggcgtcc tgccgcgccc
      961 ggtcgtcggt acggatcacc agccaggaca gcgcggccag cccgacgacg gtgatcaggg
     1021 tgtacgcggc ggtcagccgc agctgcagcc ggcgcagccg cctccgggcc ggggaacgac
     1081 gccgtacggg cttcgccatc aggacggccc cggctccgcc gacagcagga atccctgacc
     1141 gcgcacggtg tgcacgaggc cgggggagcc gagcttgcgc cgcagccccg cgacgacggc
     1201 gtccaccaca ttggacaccg ggtcggccat ctcgtcccag cagtgctcca gcaggtcggt
     1261 acgggtgacg acggcgtccc ggtggacgag gagctgctgg agcacggcgt actccttcgg
     1321 gctcagcgac agcagcacgc caccgcgccg cacctcgtac cgcgccacgt ccatgaccac
     1381 atccgcacag ctcaggaagg agggcagccg ggccgccgcc cgccggctca ggccccggac
     1441 ccgcagcacc agctcgggca tcgcgaacgg tttggccaga tagtcgtccg cgccgctctc
     1501 cagcccccgc aaccgctcgg gcagcgagtc cagggcggtg agacacagca cgggcaccga
     1561 ccagccggcc cggcgcctga cctccagcgg gacgagggtg tcgccggagg gcaccatccg
     1621 gtccagcacc acacagtcgt acgcggtgac gtacagcagc acgtccgcct ccggccagtc
     1681 cgccgcgcag tccacggcga agcccccgtc ccgcagcccg gacgcgatga cctcccgcag
     1741 gtcgtcgtcg tcctccacca gcagcacacg catggggagg acgcctccga tgtcgttcca
     1801 agggggagag acccgccatc ctaaggcgtc ccgcgcgcct gccccgaggc ggccgtcaga
     1861 ccggtgaggc ctcgatgatc cggaagatgt cgggcgcggg catcggcgtc acgctccgga
     1921 gggcgaagcc gcccgccgcg cacagggcgg cgaagtcgtc ctcggtccgc tcacgcccgc
     1981 cgacatcgac gagcatgtcg agatcgctga ggtagacgcc tcccgccggg tccggcggca
     2041 ccgtggcggg cagcaccggc tcgacgatca gcagcgaacc gtggtcgggg atgacggccc
     2101 ggatgtgccg caggatggcc gcgcacctct cgtcgtccca gtcgcggatg atgctcttca
     2161 gcatgctcct tgagatggcc gaaaccgtct cggacagcaa ctggagtccg gcgagcgcgc
     2221 gcagcagacg cagcgtcgcc tgcggatggg tgccgagaga accggcgagc gcgtcgccgc
     2281 tcagctcgcc aggaccgatc cggtcgaaca ccccgaaccg caccgccgta ccgcgtgcct
     2341 gggtggccat ctgaccgaga acgagccggg cgaccgtgcc ccgtgcctgc cgatcaaccg
     2401 ccgcctcccc gccgttgccg gtctcctggc cctgactgtc cgtcatcaca aggctctctc
     2461 tggtcggtta ggtaaacctg acctcgcccg gagagtgctg caatcaccca tacgcagggg
     2521 tgggcgggga tgaggcggac ccggtcagtc cggccggtcg ccgtcgagcg cctctgccgc
     2581 ctcggccgcc accgcatcgg ccacggccgc caaggctggt gagtccagct tccactgctg
     2641 ccagtacagc ggtacgacga cggtccggtc cgaggtgagc cgcaccagcc ggccgtcacc
     2701 cagcagcggc tccgcctgga cctcgggcac catgccccag cccatgcccg ccgtcacggc
     2761 gttcacgaac ccctccgagg tgggcacgag atgacgccgg gcgctcgccc ggggcccgag
     2821 gccgagccgg cgaacgaagg tgtcctggag atcgtcgcgc cggtcgaacc cgatcaccgg
     2881 ggcatcgccg atcagctcgc gcaacgccgt gcccgcccgc cgttccagcc accggtcggc
     2941 gaaaccggga ctggccaccg gcagataccg catccggccc agcctgcgca ccgaacagcc
     3001 cgtcaccgcc tccggggacg aggtcaccgc cgccatcacc agcccctccc gcagcagggc
     3061 ggccgtatgg tcctggtcct cgcggcgcag ctcgtagcag aggtccagct gctccggcac
     3121 ccgccgcagg gcgggcagga accaggtcgc cagggaatcg gagttgaccg cgatcgacag
     3181 gggcgtcggt gcgtccggtc cggccatccc gagcgccgtc cgcgcatcgc gctccagcct
     3241 ggccagctgg cgggcgaagc gcaccaccac ctcgccggac tcggtgggcc gcaccggctt
     3301 cgtgcgcatc agcagcaccc ggcccgtgcg ctgctccagt gccttcacgc gctggctgac
     3361 cgccgacggc gtcagctgca gggcgccggc cgccgcgtcg aaggtgcctt cgtcgaccac
     3421 ggccagcagc gtccgtacct ggtcgagagg aagctcggac atcatgaaag ctaatgatac
     3481 gtaagaatcc ttagctggac atcatattgt cccgctttgt agcgtcggcc gcatgatgaa
     3541 cgccctgacc gccgcggccg ccggattcgg caccggcctg tccctcatcg tcgccatcgg
     3601 cgcccagaac gccctggtgc tgcgccaggg cgtccgccgt gacggtgtgc tcgcggtggt
     3661 cgggatctgc gccctgtccg acgcggtgct catcgcgctg ggggtcgccg gggtcggcgc
     3721 ggtcgtcgtg gcctggccgt cggcgctgac ggcggtgggg ctgctcggcg gcctgttcct
     3781 gatcggctac ggcctgctcg ccgcacgccg cgtcctgcgc ccgtcggccc tgcacgccgc
     3841 cgacgcgccg gccacgtcgc gccggggcgt cgtgctgacc tgcctggcga tgacctggct
     3901 caacccgcac gtgtacctcg acaccgtgtt cctgctcggg tccgtcgccg ccgaccgggg
     3961 tccgctgcgc tggaccttcg gcctcggcgc ggtcctcgcc agcctgtgct ggttcgtcgc
     4021 gctcggatac ggcgcccgga tgctgagcgg ggtcctggcg cgacctgccg cctggcgggt
     4081 gctggacgcg ctggtcgccg tgatgatgat cggcctcggc gcgaaactgg cgctggacgc
     4141 ctgaacgccc ggcgcccgtt ccgcgtacga gcctgaaagc cgggtggccg ttcccccggc
     4201 tcggttcagc cgaccggatc cagcaggccg aggcgctcgg cgcgttccgg cgtcagatct
     4261 agggcggtga ggatggaggg cgtggcgata ctggggagta tgtgctgctg cagcgtgatg
     4321 tggcggcgct gcaggtcctg gtagttggtg agagtctggg agaccgcctg ggttcccgcg
     4381 aaggcggcca cgtacaggtc cgccgtctcg gtggggttga cgtgggggag tagctcgccg
     4441 ttgtgtttgg cactgctgag gaggtgatgg gtggcttcct gccagcggag aaaggggccg
     4501 ctgcggtcga ggccctgtgc ctgctggtcc atggagaggc ggacgcccgc ccgggcgagg
     4561 aggctggtct gcagacgatg gctgaacagc atgcccaggt cgatgagttc ctggagtttg
     4621 agagcctggt ccggaacggt ctgaggtggc tcctgtgcat cgagaacacc gagcgcgaga
     4681 tcttccttgg acgggaaatg gaagtagaga gcgcctttgg tgacgccgga gctcttgagt
     4741 atctcggtga tcgtcgcggc ttgatagccg tatttctcga aggcctgcgc cgctgccatc
     4801 aggattgtct ggcgggtgcg gatcgcccgg tcctgcttgg ccatcctgtc tcctttgctg
     4861 ggcgagggcc cgggaaacgg ctgggacggt gtggggggac gaccctgccg gggatagaaa
     4921 aaaaaccgct tagtctgtat cttatcagcg cacgtgcatc gcaggaacgg ctcgccatca
     4981 catgacccct ggggggagtt atgcccgaag cagcagtctt gatcgatccg gtgccggcta
     5041 tggattcgga ggccgaggtg gtgcatcccg tggggatcga catggtgcac cggtccaggc
     5101 cggaggacgc ctttccgcgt aactgggtgc gcctggcaag cgatcggttc tcggtcgcgg
     5161 cggtcctgcc gcacgatcat ccgttcttcg ccccggtgag cggcgaccgg cacgatccgc
     5221 tgctggtggc ggaggcgatg cgacaggcgg ccatgctctc cttccacgcc gggtacggcg
     5281 tcccggtggg ctaccacttc ctgatggcgg acctcgacta cgcgtgcgac ctggaccact
     5341 tgggcgtcgg cggcgccccc gccgagatcg acgtggaggt gttctgctcc gatctgaagt
     5401 ggcggggcgg actgcccgta caggggcgcg tgggctgggc ggtccaccgc gacggggaac
     5461 tggccgcgac ggggacgggc gcgacccgct tcacgaaccc caaggtgtac cggcggatgc
     5521 gcggtgacac acccgccgag gggatagcga taccgcaggc gacgccggtc tccaccgccg
     5581 gggcgggccg caccagggcc gaggacgtgg tgctctcgga gaccgggcgg gacggggtgt
     5641 gggaactgcg cgtggacacc cggcatccga cgctgttcca gcgacccaac gaccatgtcc
     5701 cgggcatgct gctgctggag gcggcccgtc aggcggcctg cctggcggcg gggcccggcg
     5761 gaatcgttcc ggcggaggcg cgcagccggt tccaccggta cgccgagttc gacagcccgt
     5821 gttcgatcgg ggcggtggtc cagccgggga cggccgagga tacggtgacg gtccaggtgg
     5881 caggccatca gggcggcgaa ccggtgttct ccaccgtgct gtccggcccg cgggtcaatg
     5941 gctgagaggt tttccgagtg attctcgtga ccggggcgac cggagctgtg ggccgtgagg
     6001 tcgccggaca gctggcggcg gcaggcccgg tgcggattct cgcgcgcagt cccgagcggc
     6061 tgacggtacg agggagcggg gtcgaggtcg tcgaggggga gtacggggac cgctccgccc
     6121 tcgaccgggc catgcggggc gtcacctcgg tcttcctggt gacgaagagt cccacggagc
     6181 cccacgacga gtgggtggcc gctgcggcgg cagcggcggg cgtccgccac ctggtcaagc
     6241 tgtccatgat ggcggtggag gagccggggg ccgacgactt catcacccgg cgtcagcggg
     6301 agaacgaaca ggccgtgcgg gagtcggggg tcgcctggac gttcgtccgt cccaggacgt
     6361 tcatgtccaa cacgctgtcc tgggcgcccg gcatccggtc ggccggagtg gtgcgcgccc
     6421 tgtacggcga cgcgccggtc gcatgcgtcg acccgcgcga cgtcgcggcg gtggccgtcg
     6481 ccgcgctcac cggtacgggg cacgagggca gggcgtacgc cgtgtcgggc ccggaggcga
     6541 tcaccgcgcg ggagcagacg gccgagctct cccgggtgct ggagcggccg ttgcgcttcg
     6601 aggaactggg cgtggacgcc gcgcgcacgg ccctgctggc gtcgtacccg cgccccgtcg
     6661 cggaagcctt cctggagagc gcggagcggc agcgggcggg cgcgaaggct acggtggtgc
     6721 ccaccgtcga ggaactgaca gggcgtccgg cccggccgtt ccggatctgg tcggccgatt
     6781 acgcggacgc gttcgcggtg tagtaccgcg tgacagatcg caaatacgtg cgatgatccg
     6841 ctctttccct ttccggcccg gtttcatggc ccgaagtgca gagatttcat ggcccgaagg
     6901 gaagaagtgg ccggaaagga tcccggaagc ctccgccggc ttcttccgtt ctctggaaat
     6961 gaaaccggta agttggtttg ataactccat gaagccgtcc cactgccccg gcgagccctc
     7021 caccggggca gccgcatttt ccgttcccct gtccggtcgc cgattcttcg agcggagtcc
     7081 ccggtgacca aacaggagcg ggccacccgt acccgccggt cgctgatccg atccgccgcc
     7141 accgtcttcg agcagcacgg ctacgcgcag gcgcgtctga cgctgatcag ttccggcgcg
     7201 ggcgtcagca ccggcgcgct gcacttccac ttcgagaaca aggccgcggt cgcggaagcg
     7261 gtggtggtgg aggcgtcccg ggagctgcgc gagatgtccg gcgcgatccg gcgcaggacg
     7321 gacaccgccc tgcaggcgct cgtcgacagc tcgcatgctc tcgcggaacg gctgcgcgag
     7381 gacccggtga gccgggccgg attccggctg agctgcgacg ccgcgagaac cgccgccccg
     7441 gacctgaggg tgcagtggca ccaccgcgtg cgggaactgc tggacgaggc ggccgccgac
     7501 ggaacactcg ccgaggacgt ctcccgcgag gacgcggcgg ccgccacggt cgccgcgacg
     7561 gccgggttcg aggtgctcgg ccggcacgat tccgggtggc tctccccgta catgctcacc
     7621 ggcttctggc agttgctgct gccccggctc gccgccgcac agaccctgcc gctcctggac
     7681 ccggccggca cggttgcgac ggcgtcgacc cggattcccg cgcctgcggc gtgcgccacc
     7741 gatgaggcgg aggcggcggc aggagtggcg cccgaggagg tggcggcggt ggccgaggcc
     7801 gactgaccgg tcggccggcc cggcgcgtgc cgatgctccg cccgctcggt accttccgcc
     7861 gcatggctga cgagatgttc gcggatcccc gactggctgc gctctacgat ccgctcgacc
     7921 ccgaccgagg cgatctggac ccgtacgtcc gcatcgccga ggagttcggt gcacggcggg
     7981 tgctggacat cggctgcggt accggagtgt tcgccctgct gctggcggac cgaggggtcg
     8041 aggtcgtcgg cgtcgacccc gcccgggcct cggtcgacgt ggcgcgcgcc aagccgggcg
     8101 gtgaccgggt ccgctggatc agcggtgacg cttcggctct tccgccgttg cgggtcgacc
     8161 tggccaccat gacggccaac gtggcacagg agatcaccga cccgcgcgac tggcgggcga
     8221 cgctgcgcgg tgcctacgag gcgctacggc cgggcgggca cctggtcttc gagacgcgag
     8281 acccggcccg gcgcgcctgg gaggcgtgga accgggagcg ttcgcaccgt gtcaccgaga
     8341 tcatcggcct cggccccgtc gagagctggg tcgaggtgac cggggtcgcc gggccgctcg
     8401 tgtcgttccg ttggacctac gtcttcgcgg tcgacggccg ggtcctcacc tccgactcca
     8461 cgctgcggtt ccgcgagcgt caggaggtcg aggcggacct gacggcccgg ggcttcgtcg
     8521 tgcgcgaggt gcgtgacgca cccgaccggc cgggccggga gttcgtcctc atcgctcgtc
     8581 gtccggaccc cgacccgcgc cgctgatcac atccgcccgg cccgcgtcgg cgccccagct
     8641 cgccagcagg cgcagggcct gggccgactc cgaacccggc tccgcgtggt aggtcaccag
     8701 ctgctggccc tcgtcgtccg ggagattcag cgtctcgtac gacagggtca gatcgccgac
     8761 gagcggatgc cggatcagct tgatgccgtg gcccttctcc ttgacgttgt gcgtggccca
     8821 cagccgccgg aactcctcgc tcttcagcga gagttcgccc accagcgccg acagctgcgg
     8881 atcgtcgggg tggcagcccg cgtacagccg gagatagctg accatgtccg aggccttgga
     8941 ttcccagttc aggaacaggt cccggtaggc gggggagagg aacaccagac gcgcccagtt
     9001 ccgctcggcc tgcggcagtg cggcccagtc gccgaacacc gccgccgcca tcggattcca
     9061 ggcgaggatg tcggaccgcg ccccggccac gtacgcggga atgccctcca tgttgtcgag
     9121 gagatacagc agcccggtcc gcacccgctg caccttcgcc gggcggcgcc tcttcttgtg
     9181 ccgggccggc ctcgccagat ggttcaggtg cgcgtgctcg gcgtcggtga gccgcagcgc
     9241 gcgggcgatc gcgtcgagca cctcggccga cacattgcgt ccgttgccct gctccagacg
     9301 cgtgtagtac gccacggaca ccccggccag ctgcgccagc tcctcccggc gcagcccggg
     9361 cacccgacgg gcccggccga actccggcag tcccacgtcc tgcggctgga gcttggccct
     9421 gcgggtgcgc aggaactcac tgagttcggc acgccggtcc agcggctcgg gtgcggcggc
     9481 ctccggctga tcgtccatgg gttccattat tcctgcgatg cgtggtcgta ggcggccgag
     9541 cctgtccccg tcagtagtag gaccactgga cgtaggcaga gcggtgggct gggtaaacgt
     9601 cggacacgga gtgaggctgg gacacgttcc taccgcatca cccagaggag aacctccggc
     9661 atgaccaccg ttgccgccta cgccgctccc cgtgccaagg ccccgctgga gcgcaccacg
     9721 atcgagcgtc gtccggtggg cgagttcgac atcctgatcg acatcaagtt cgccggtatc
     9781 tgccactccg acatccacca ggcccgcgac ggctggggcg agggcatctt cccgatggtg
     9841 cccggccacg agatcgccgg gatcgtcgcc gagaccggct ccggggtcac ccggttcaag
     9901 gtcggcgacc gcgtcggcgt cggctgcatg gtcgactcct gcggaaagtg cgacgcctgc
     9961 ctgatgggcc gcgagcagca ctgtgccgag ggcaacaccc agacgtacaa cgccctcgac
    10021 cgcagcggcg agcccaccta cgggggctac tcgacgcaca tcgtcgtgac cgagaagttc
    10081 gccgtcacca tccccgaggg catcgccctc gacgaggccg cgccgctcct gtgcgccggc
    10141 atcaccacgt actcgccgct gaagcgctgg ggcgcgggac ccggcaagaa ggtcgccgtc
    10201 gtcggcctcg gcggtctcgg ccacatggcc gtcaagatcg cgcacgcgct cggcgccgag
    10261 gtcacggtcc tctcccagtc cttgcggaag aaggacgacg ggctcaagct gggcgccgac
    10321 cactactacg cgaccagcga ccccgagacc ttcgaggagc tggccggcac cttcgacgtc
    10381 atcctctcca cggtctcggc cccgctggac ttcggcgcct acctcggcct cctgcgcacg
    10441 gacggcaccc tcgtcaacgt gggcgccccc gaggagccga tctcgctgaa cctcttctcc
    10501 ctgatcatgg gcaaccggtc gatcgcgggt tcggcgatcg gcggcatcgc ggagacccag
    10561 gagatgctgg acttctgtgc cgtgcacggg ctcggcgccg agatcgaggt gatcggcgcg
    10621 agccaggtca acgaggcgta cgagcgggtt ctcgcgagcg acgtccgcta ccgcttcgtg
    10681 atcgacacgg cgaccgtctg agccaccacg gcccgccccc accgtgcggg cggccgcccg
    10741 gccgtgtcct cctcgccggc gtccgccccn nnnnnngggc ggacgccttt ctcgtccggc
    10801 gcacagtgcc cgaccgatcg ccgggcatgg accgatctag cctcccgcgc cccgagccct
    10861 tcgccatcgc gcgggcggcc cggtcccggc cgacaatggc cggaggcagt cctgagggcg
    10921 tcaccgggga ccggcccggg agagg
//
