LOCUS       NZ_JALDPQ010000062     10399 bp    DNA     linear   CON 28-MAY-2024
DEFINITION  Streptomyces sp. TRM75563 733, whole genome shotgun sequence.
ACCESSION   NZ_JALDPQ010000062
VERSION     NZ_JALDPQ010000062.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. TRM75563
  ORGANISM  Streptomyces sp. TRM75563
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 396394)
  AUTHORS   Chen,Y.
  TITLE     Streptomyces aheensis sp. nov., isolated from road
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 396394)
  AUTHORS   Chen,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2022) College of Life Science, Tarim University,
            Hongqiao, Alar 843300, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 14-MAR-2022
            Assembly Method                   :: ABySS v. V2.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 400.0x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022748615.1-RS_2024_05_28
            Annotation Date                   :: 05/28/2024 05:18:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,476
            CDSs (total)                      :: 7,413
            Genes (coding)                    :: 7,165
            CDSs (with protein)               :: 7,165
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 56
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 249
            CDSs (without protein)            :: 248
            Pseudo Genes (ambiguous residues) :: 1 of 249
            Pseudo Genes (frameshifted)       :: 108 of 249
            Pseudo Genes (incomplete)         :: 202 of 249
            Pseudo Genes (internal stop)      :: 19 of 249
            Pseudo Genes (multiple problems)  :: 75 of 249
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 19:22:52
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 318513
            Orig. end                         :: 328912
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JALDPQ010000062.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10399
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="1"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10399
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10399
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5399](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5399)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            133..1191
                     /locus_tag="MQC87_RS19280"
                     /old_locus_tag="MQC87_19280"
     CDS             133..1191
                     /NRPS_PKS="Domain: Aminotran_5 (19-347). E-value: 1.8e-24.
                     Score: 78.3. Matches aSDomain:
                     nrpspksdomains_MQC87_RS19280_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012381431.1"
                     /locus_tag="MQC87_RS19280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MQC87_19280"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_242740640.1"
                     /sec_met_domain="Aminotran_5 (E-value: 3.1e-18, bitscore:
                     60.3, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTILAPIQAAAVEFAPEVTYLNTSSWGLLPRRTIAAVKALADENA
                     VGRREGAGSFDAVEAARVGFARLAGVHPDRVATGSSVTVHVGLIAASLPPGAEVLCPEG
                     EFASVVSPFALRGDLRVRYAPLADLAGAVRPSTTLVAFSAVQSADGRVADLAAVRAAAA
                     AHGARTLLDATQAAGWFPLDAGAYDYTVTGGFKFLLCPRGTSFLTVTEEAQDTLPPLFA
                     GWVSAGAPWASNYGPLERLAPTARGFDEPPAFLSYHGAEHSLDLLAEVGSDALYAHATG
                     LAARLRAGLTRLGREVVPGESAIVSVPGLQGRRDDLMKAGIAVSAPAGNLRISCHLYNT
                     EADVDRVLEFLA"
     aSDomain        190..1173
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MQC87_RS19280_Aminotran_5.1"
                     /evalue="1.80E-24"
                     /label="MQC87_RS19280_Aminotran_5.1"
                     /locus_tag="MQC87_RS19280"
                     /protein_end="347"
                     /protein_start="19"
                     /score="78.3"
                     /tool="antismash"
                     /translation="YLNTSSWGLLPRRTIAAVKALADENAVGRREGAGSFDAVEAARVG
                     FARLAGVHPDRVATGSSVTVHVGLIAASLPPGAEVLCPEGEFASVVSPFALRGDLRVRY
                     APLADLAGAVRPSTTLVAFSAVQSADGRVADLAAVRAAAAAHGARTLLDATQAAGWFPL
                     DAGAYDYTVTGGFKFLLCPRGTSFLTVTEEAQDTLPPLFAGWVSAGAPWASNYGPLERL
                     APTARGFDEPPAFLSYHGAEHSLDLLAEVGSDALYAHATGLAARLRAGLTRLGREVVPG
                     ESAIVSVPGLQGRRDDLMKAGIAVSAPAGNLRISCHLYNTEADVDRV"
     gene            complement(1221..1727)
                     /locus_tag="MQC87_RS19285"
                     /old_locus_tag="MQC87_19285"
     CDS             complement(1221..1727)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1284:
                     transcriptional regulator, MarR family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018957688.1"
                     /locus_tag="MQC87_RS19285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MQC87_19285"
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="WP_103510681.1"
                     /transl_table=11
                     /translation="MTTGHAAPAEEPCTDALPTAALSGPVSHAVSRVARLHRIAASKAL
                     KGAELYPGQELLMMHLWDRGAVRQTELIRSLELDPSTVTKMLQRLEQTGHVRRRPDPAD
                     RRAVLVEATDESCALHTAVEAAWGSLEEHTLAGLDAAERAELVRLLAKVEENLCRETAD
                     CPDRR"
     gene            1923..2993
                     /locus_tag="MQC87_RS19290"
                     /old_locus_tag="MQC87_19290"
     CDS             1923..2993
                     /GO_function="GO:0010181 - FMN binding [Evidence IEA]"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1217: NADH:flavin oxidoreductase/NADH oxidase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003969934.1"
                     /locus_tag="MQC87_RS19290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MQC87_19290"
                     /product="alkene reductase"
                     /protein_id="WP_242740641.1"
                     /transl_table=11
                     /translation="MTTVFDPIDLSGTPLANRIALAPMTRSRAGEGGTATELTVTYYAQ
                     RASAGLVITEGIQPSVVGQGYPSTPGLHSAEQVASWRTVTDAVHAKGGRIFAQIMHAGR
                     IGHPVLLPDGLVPVSASPVRAEGQVYTHVGPKDFVEPHELTDAEVRATVADFVTAARNA
                     VEAGFDGVELHGANGYLIHQFLAPNTNRRTDAWGGSDEARTRFAVEVVKAVAAEIGPER
                     TALRISPGNPYNDIDEPAPEATYTALVREIEPLGLAYLHVLEAVPIRELTLALRKAFSG
                     TFVINVHTEGATGPDDHTVIDDGLADLISYGALFLANPDLPARLKAGGPFNTPDPSTFF
                     GGDAKGYTDYPALDAV"
     misc_feature    1924..1938
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 17.18"
                     /tool="antismash"
     gene            complement(2969..4003)
                     /locus_tag="MQC87_RS19295"
                     /old_locus_tag="MQC87_19295"
     CDS             complement(2969..4003)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010070337.1"
                     /locus_tag="MQC87_RS19295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MQC87_19295"
                     /product="VOC family protein"
                     /protein_id="WP_242740642.1"
                     /transl_table=11
                     /translation="MSSHVRHITIDCSDAYGLGAFWAQVLDAPLSDEDSPGDPEALVEA
                     PGAAILFVQVDDPKQTKNRVHLDIQPQDRSRDEEVERLLALGATLVGDHRRPNGRGWVT
                     LADPEGNEFCVECGAAERAALTGARLPVTADDVTSAVRLAVDVLAGAPADRWDGPAGSL
                     TWTCWETAEHLSDDLFAYAVQLGPRTPPLDGEVPYRWAPDRQGGPHNAVFADRAAGPAG
                     LLATLEASGALLASMARTTPPGVRSYHGYGISDPEGFAAMGVVETLVHTHDLAEGLGLT
                     WAPPGALCDRVLARLFPDAPAGGDRWTVLLWATGRAELPDHPRRTSWRWDGRPPADQTA
                     SSAG"
     gene            complement(4104..4997)
                     /gene="thpD"
                     /locus_tag="MQC87_RS19300"
                     /old_locus_tag="MQC87_19300"
     CDS             complement(4104..4997)
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014156966.1"
                     /locus_tag="MQC87_RS19300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MQC87_19300"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_103510678.1"
                     /sec_met_domain="PhyH (E-value: 7.9e-40, bitscore: 131.8,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTEVRADLYPSRGAAEMTTPRQDPVIWSAPGAPGPIAAKDLQSY
                     EHDGFLSIEQLITPDEVAVYQAELNRLISDPAIRADERSIVEKQTQNVRSVFEVHRISE
                     VFAGLVRDERVVGRARQILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLPNM
                     RTVSVSIALTENFDTNGGLMIMPGSHKSFLGCAGETPKDNYKKSLQMQDAGTPSDEALT
                     KMADRHGIRLFTGKAGSATWFDCNAMHGSGDNITPYARSNVFIVFNSVENAAEEPFAAP
                     IRRPEFIGARDFTPVK"
     gene            complement(5001..5399)
                     /locus_tag="MQC87_RS19305"
                     /old_locus_tag="MQC87_19305"
     CDS             complement(5001..5399)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006123624.1"
                     /locus_tag="MQC87_RS19305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MQC87_19305"
                     /product="ectoine synthase"
                     /protein_id="WP_006123624.1"
                     /sec_met_domain="ectoine_synt (E-value: 6.6e-55, bitscore:
                     178.9, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFSDIENTDRHVKAASGTWESKRIVLAKEKVGFSLHETVLY
                     AGTETSMWYANHIEAVLCTEGEAELTNDETGEKHWITPGTMYLLDGHERHTMRPKTDFR
                     CVCVFNPPVTGREDHDENGVYPLLTEEG"
     gene            complement(5489..6751)
                     /gene="ectB"
                     /locus_tag="MQC87_RS19310"
                     /old_locus_tag="MQC87_19310"
     CDS             complement(5489..6751)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-363). E-value: 2.8e-72.
                     Score: 235.5. Matches aSDomain:
                     nrpspksdomains_MQC87_RS19310_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014156968.1"
                     /locus_tag="MQC87_RS19310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MQC87_19310"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_242740643.1"
                     /sec_met_domain="Aminotran_3 (E-value: 2.1e-78, bitscore:
                     258.2, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITPPALSVFETLESEVRSYCRGWPAVFDRAQGARLTDEDGHSY
                     LDFFAGAGSLNYGHNNPVLKRALIDYIERDGITHGLDMATTAKRAFLETFQNVILRPRD
                     LPYKVMFPGPTGTNAVEAALKLARKVKGRESVVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDNYFDGTVPDFLWFERLLEDQGSGLNKPAAVIVETVQGEGGINVARP
                     EWLRALRELCDRQDMLLIVDDIQMGCGRTGGFFSFEESGVVPDIVTLSKSISGYGLPMA
                     LCLFKPELDIWEPGEHNGTFRGNNPAFVTAAAALDAYWADGQMEKQTLARGEQVEQTLL
                     AICAEEPTAQFRGRGLVWGMEFEDPARASSVCARAFELGLLLETSGPQSEVVKLLPPLT
                     ITPEELDEGLRTLARCVRETA"
     aSDomain        complement(5663..6667)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MQC87_RS19310_Aminotran_3.1"
                     /evalue="2.80E-72"
                     /label="MQC87_RS19310_Aminotran_3.1"
                     /locus_tag="MQC87_RS19310"
                     /protein_end="363"
                     /protein_start="28"
                     /score="235.5"
                     /tool="antismash"
                     /translation="FDRAQGARLTDEDGHSYLDFFAGAGSLNYGHNNPVLKRALIDYIE
                     RDGITHGLDMATTAKRAFLETFQNVILRPRDLPYKVMFPGPTGTNAVEAALKLARKVKG
                     RESVVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDNYFDGTVPDFLWFER
                     LLEDQGSGLNKPAAVIVETVQGEGGINVARPEWLRALRELCDRQDMLLIVDDIQMGCGR
                     TGGFFSFEESGVVPDIVTLSKSISGYGLPMALCLFKPELDIWEPGEHNGTFRGNNPAFV
                     TAAAALDAYWADGQMEKQTLARGEQVEQTLLAICAEEPTAQFRGRGLVWGMEFE"
     gene            complement(6948..7478)
                     /gene="ectA"
                     /locus_tag="MQC87_RS19315"
                     /old_locus_tag="MQC87_19315"
     CDS             complement(6948..7478)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015607778.1"
                     /locus_tag="MQC87_RS19315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MQC87_19315"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_242740644.1"
                     /transl_table=11
                     /translation="MTAAPADFARARSEFLSIDTPRVEDGAAIWRIARDSQVLDLNSSY
                     SYLLWCRDFAATSVVARGENGEPIAFVTGYIRPDRPRTLVVWQVAVDQAHRGKGLAATL
                     LDALTARVAADQGLSSVETTITPDNTASDRLFTSYAQRHDVALEQEVLFEGALFPEGTH
                     LPEVLYRIGPFAA"
     misc_feature    7489..7503
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.51"
                     /tool="antismash"
     misc_feature    complement(7503..7515)
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 17.04"
                     /tool="antismash"
     misc_feature    complement(7634..7649)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     misc_feature    7644..7659
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.62"
                     /tool="antismash"
     misc_feature    complement(7644..7661)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 14.75"
                     /tool="antismash"
     misc_feature    7719..7735
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: medium, score: 21.81"
                     /tool="antismash"
     misc_feature    7776..7789
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 13.67"
                     /tool="antismash"
     gene            complement(7787..8893)
                     /locus_tag="MQC87_RS19320"
                     /old_locus_tag="MQC87_19320"
     CDS             complement(7787..8893)
                     /NRPS_PKS="Domain: Aminotran_5 (16-284). E-value: 1.7e-27.
                     Score: 88.2. Matches aSDomain:
                     nrpspksdomains_MQC87_RS19320_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006123620.1"
                     /locus_tag="MQC87_RS19320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MQC87_19320"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_242740645.1"
                     /sec_met_domain="Aminotran_5 (E-value: 1.6e-22, bitscore:
                     74.4, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTHPFLDLPPLTADRFAAIERSVARLLATEQDVVITQGEALLPLE
                     GCIRSGARPGSTALNVVTGPYGQTFGNWLRDCGAQVVDLAVPFHTAVTAGQVAEALAAH
                     PEIDFVSLVHAEAATGNTNPVAEIGEVVRAHGALFMLDAVASVGAEPLLPDAWGVDLCV
                     IGAQKAMGGPAGVSAVSVSARAWERMAANPAAPRRSYLSLLDWKERWIDGGRKALPHAP
                     AQLEMLGLEACVARIEDAGLGTVMARHASAAAATRAGAVALGGGLEPYVHEARDAAPVA
                     TTLRAPAGTDAAALVAHALAADPSLPLVAGGGALSKEMIRVNHYGADATRDAVLSSLAA
                     LGAALTDAGRRVDIDAARVAVSETWPGR"
     aSDomain        complement(8042..8845)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MQC87_RS19320_Aminotran_5.1"
                     /evalue="1.70E-27"
                     /label="MQC87_RS19320_Aminotran_5.1"
                     /locus_tag="MQC87_RS19320"
                     /protein_end="284"
                     /protein_start="16"
                     /score="88.2"
                     /tool="antismash"
                     /translation="AAIERSVARLLATEQDVVITQGEALLPLEGCIRSGARPGSTALNV
                     VTGPYGQTFGNWLRDCGAQVVDLAVPFHTAVTAGQVAEALAAHPEIDFVSLVHAEAATG
                     NTNPVAEIGEVVRAHGALFMLDAVASVGAEPLLPDAWGVDLCVIGAQKAMGGPAGVSAV
                     SVSARAWERMAANPAAPRRSYLSLLDWKERWIDGGRKALPHAPAQLEMLGLEACVARIE
                     DAGLGTVMARHASAAAATRAGAVALGGGLEPYVHEARDAAPVATTL"
     gene            8982..10082
                     /locus_tag="MQC87_RS19325"
                     /old_locus_tag="MQC87_19325"
     CDS             8982..10082
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006123619.1"
                     /locus_tag="MQC87_RS19325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MQC87_19325"
                     /product="amidohydrolase family protein"
                     /protein_id="WP_103510674.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 4.7e-19, bitscore:
                     63.2, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDQEEQRVLRVKGRVLVGPDEVRDELWAVDGRITYERPPGADRA
                     VTVTGWALPGLVDAHCHVGLDAHGPVDAATAEKQALTDREAGTLLIRDAGSPSDTRWVD
                     DREDLPKIIRAGRHIARTRRYIRNYAHEIEPGDLVAYVAREARRGDGWVKLVGDWIDRD
                     AGDLTACWPRGELAAAIAEAHRLGARVTAHCFAEESLTDLVEAGIDCIEHATGLTEETI
                     PLFAERGVAIVPTLVNIATFPDLAAGGEAKFPRWSAHMRQLYERRYDTVRAAYDAGIPV
                     YVGTDAGGSLAHGLVAGEVAELVKAGIPPLEALSATAWGARRWLGRPVLEEGAPADLVV
                     YEEDPRADVRVLAAPRHIVLNGRVIG"
ORIGIN
        1 acggggcctg cgaggtcccg gggagctgag ctccagcggg ggcggttcat cggtgatgca
       61 taagcgatcc ttgggtgcac cccttaactc tcggtaattg actttgggtt gagggggctt
      121 cagggtggac gtatgacgat cctcgcgccc atccaagccg cagccgtcga attcgcgccc
      181 gaggtcacct acctcaacac ctccagctgg ggcctgctgc cccgccggac gatcgccgcc
      241 gtcaaggccc tggccgacga gaacgccgtc ggccggcggg agggggcggg cagcttcgac
      301 gcggtggagg ccgcccgggt cggattcgcc cggctcgccg gggtccaccc ggaccgggtc
      361 gccaccggca gttccgtcac cgtccacgtc ggcctgatcg ccgcttcgct gccgcccggc
      421 gccgaagtcc tctgccccga gggcgagttc gcctccgtgg tcagtccgtt cgcgctccgc
      481 ggcgatctcc gcgtgcggta cgcgccgctc gcggacctcg ccggggccgt acggccctcg
      541 accaccctgg tcgccttctc cgcggtgcag tcggccgacg gccgggtcgc cgacctcgcc
      601 gccgtccgcg cggccgccgc ggcgcacggg gcccggaccc tgctggacgc cacccaggcg
      661 gcgggctggt tcccgctgga cgccggggcg tacgactaca ccgtcaccgg cggcttcaag
      721 ttcctgctct gcccgcgcgg tacgtcgttc ctgacggtca ccgaggaggc gcaggacacc
      781 ctgccgccgc tcttcgccgg ctgggtctcc gccggtgcgc cctgggcgag caactacggt
      841 ccgctggagc gcctcgcccc gaccgcccgc ggcttcgacg agccgcccgc cttcctctcg
      901 taccacgggg ccgagcactc cctggacctg ctcgcggagg tcggctccga cgccctgtac
      961 gcgcacgcca ccgggctcgc ggcccggctg cgcgccgggc tgaccaggct cgggcgcgag
     1021 gtggttccgg gtgagtcggc gatcgtctcc gtgccagggc tgcagggccg tcgggacgat
     1081 ctgatgaagg ccgggatcgc cgtctcggcc ccggccggga atctgcggat ctcctgccac
     1141 ctctacaaca ccgaggccga tgtggaccgg gtgctggagt tcctggcctg atcgctccgt
     1201 gcgtccgggc gtcgtacggc tcagcgccgg tcggggcagt ccgcggtctc ccggcagagg
     1261 ttctcctcga ccttcgccag cagtcgcacc agctccgcgc gctcggccgc gtccaggccc
     1321 gcgagcgtgt gctcctccag gctgccccag gcggcctcga ccgcggtgtg cagcgcgcag
     1381 ctctcgtccg tcgcctcgac cagtacggcc cgccggtcgg ccgggtcggg gcggcggcgc
     1441 acatgcccgg tctgctccag ccgctggagc atcttcgtga ccgtggacgg gtccagctcc
     1501 agcgacctga tcagctccgt ctgacggacc gccccccggt cccacaggtg catcatcagc
     1561 aactcctgcc ccgggtagag ctccgccccc ttgagcgcct tggacgccgc gatccggtgc
     1621 agccgggcca cccgggagac ggcgtggctc acgggcccgc tgagggccgc cgtgggcagc
     1681 gcgtcggtac agggctcttc cgcgggtgcg gcgtggccgg tggtcatggc gggctcctgg
     1741 ggtcctcggg ccggacgggc cctggcgggg gctttcggtg gcggcttccg atgatgcccg
     1801 accgaatgcc ggactgagcg cccgacggat tcccacgact ttaccttggt cggccaatga
     1861 atgggctatg gtggccgtcg gcgccgatgc ttggccgacc acatactttc ttgggagcct
     1921 tcatgaccac cgttttcgac ccgatcgacc tgtccggtac cccgctggcc aaccgcatcg
     1981 ccctggcccc gatgacccgc agccgagccg gtgagggcgg caccgccacc gagctcaccg
     2041 tcacctacta cgcccagcgc gcctcggccg gcctggtgat caccgagggc atccagccgt
     2101 ccgtcgtggg ccagggctat ccctccacgc ccgggctgca cagcgccgag caggtcgcct
     2161 cctggcgtac ggtcaccgac gcggtgcacg cgaagggcgg caggatcttc gcccagatca
     2221 tgcatgcggg ccgcatcggc cacccggtcc tgctccccga cggcctcgtc cccgtcagcg
     2281 cctccccggt cagggccgag gggcaggtct acacccacgt cggcccgaag gacttcgtcg
     2341 agccgcacga gctgaccgac gccgaggtcc gcgcgaccgt cgccgacttc gtcaccgccg
     2401 cccgcaacgc cgtggaggcc ggattcgacg gggtcgagct gcacggcgcc aacggctacc
     2461 tcatccacca gttcctcgcc cccaacacca accggcgcac cgacgcgtgg ggcggctccg
     2521 acgaggcccg cacccgcttc gccgtcgagg tcgtcaaggc cgtcgccgcc gagatcggcc
     2581 cggagcgcac cgcactgcgg atctcgcccg gcaacccgta caacgacatc gacgagcccg
     2641 cgccggaagc cacgtacacc gcgctcgtcc gggagatcga gccgctcggc ctcgcctacc
     2701 tgcacgtcct ggaggccgtc ccgatccgcg agctgaccct cgccctgcgc aaggccttct
     2761 ccgggacgtt cgtcatcaac gtgcacaccg agggcgccac cggacccgac gaccacacgg
     2821 tgatcgacga cggcctcgcc gacctgatct cctacggggc gctcttcctc gccaacccgg
     2881 acctgcccgc ccggctgaag gcgggcggcc cgttcaacac ccccgacccg tcgaccttct
     2941 tcggcggcga cgccaagggc tacaccgact acccggcgct cgacgccgtc tgatccgccg
     3001 ggggccgccc gtcccaccgc catgaggtgc ggcgcgggtg gtccggcagc tcggcgcggc
     3061 cggtggccca cagcaggacg gtccaccggt ccccgcccgc cggggcgtcg gggaagagcc
     3121 gggccagcac ccggtcgcag agcgcgccgg gcggcgccca cgtcagcccc aggccctcgg
     3181 ccaggtcatg ggtgtgcacc agggtctcca ccacacccat cgctgcgaag ccctccgggt
     3241 cggagatccc gtagccgtgg tacgagcgga ccccgggcgg cgtcgtccgc gccatcgagg
     3301 ccagcagcgc cccgctcgcc tccagcgtgg ccagcagacc ggcgggcccg gcggcacggt
     3361 cggcgaagac cgcgttgtgc ggtcccccct gccggtcggg cgcccaccgg tagggcacct
     3421 cgccgtccag cgggggcgta cgagggccca actgcaccgc gtacgcgaac agatcgtcgc
     3481 tcaggtgctc cgccgtctcc cagcaggtcc acgtgaggct tccggcgggc ccgtcccagc
     3541 ggtccgccgg ggctccggcc agcacatcca ccgcgagccg caccgccgag gtcacatcgt
     3601 cggccgtcac cggcagccgg gcgccggtca gcgccgcgcg ctcggccgcg ccgcactcca
     3661 cacagaactc gttgccctcg ggatcggcga gcgtcaccca gccccgcccg ttcggtctgc
     3721 ggtggtcgcc caccagcgtg gccccgagcg cgagcagccg ctcgacctcc tcgtcccggg
     3781 agcggtcctg cggctggatg tccaggtgga cccggttctt ggtctgtttc ggatcgtcca
     3841 cctggacgaa gaggatcgcc gcgccgggcg cctcgaccag tgcctccgga tcaccggggg
     3901 agtcctcgtc ggagagcggc gcatcgagca cctgggccca gaaggcgccg agtccgtagg
     3961 cgtcggaaca gtcgatcgtg atgtggcgta cgtgggaact catggcggac accctgtccg
     4021 ctgacccggc cccggtccag cgagttacgc aggcggggcc gggacggcgt accgtcccgg
     4081 ccccgactct cctgtgcgta ccgctacttc accggcgtga agtcccgcgc cccgatgaac
     4141 tcggggcggc ggatcggagc cgcgaaaggc tcctcggccg cgttctccac gctgttgaag
     4201 acgatgaaga cattgctgcg cgcgtacggg gtgatgttgt cgcccgaacc gtgcatggcg
     4261 ttgcagtcga accaggtcgc cgaaccggcc ttgcccgtga agagcctgat gccgtgacgg
     4321 tccgccatct tcgtcagcgc ctcgtcggac ggggtgcccg cgtcctgcat ctggagcgac
     4381 ttcttgtagt tgtccttcgg cgtctcgccc gcacagccga ggaacgactt gtgcgaaccg
     4441 ggcatgatca tcagcccgcc gttggtgtcg aagttctcgg tcagcgcgat cgacacggac
     4501 accgtccgca tgttcggcag accgtcctcc gcgtgccagg tctcgaagtc cgagtgccag
     4561 tagaagcccg aggccccgaa gcccggcttc acattgatcc gggactggtg gacgtacacg
     4621 tccgagccga ggatctggcg ggcccggccc accacccgct cgtcgcggac cagcccggcg
     4681 aagacctcac tgatccggtg gacctcgaag acggaccgca cgttctgcgt ctgcttctcg
     4741 acgatggagc gctcgtcggc gcggatcgcc gggtcggaga tcagccggtt cagctccgcc
     4801 tggtagacgg cgacctcgtc cggcgtgatc agctgctcga tgctgaggaa gccgtcgtgc
     4861 tcgtagctct ggaggtcctt ggcggcgatc ggacccggtg cgcccggcgc ggaccagatg
     4921 accgggtcct ggcggggagt ggtcatctcg gcggcgccgc gcgaggggta gagatcggcg
     4981 cgtacttcgg tggtcatggt tcagccctcc tcggtcagca gtgggtagac accgttctcg
     5041 tcatggtcct cccgtccggt gacgggggga ttgaagacgc acacgcagcg gaagtcggtc
     5101 ttgggccgca tggtgtgccg ctcatgtccg tccagcagat acatcgtgcc gggcgtgatc
     5161 cagtgcttct cgccggtctc gtcgttcgtg agctcggcct cgccctcggt gcacagcacc
     5221 gcctcgatgt gattcgcgta ccacatggag gtctccgtgc ccgcgtacag cacggtctcg
     5281 tggagcgaga agcccacctt ctccttggcg agcacgatgc gcttgctctc ccaggtgccg
     5341 gacgcggcct tcacatgccg gtcggtgttc tcgatgtcac tgaacgatcg gacgatcacg
     5401 gtgagcggtt gcctttctct tctctgcacg ggccccgggc ctcgcggccc ggggcctctt
     5461 cttctgcgcc tgccccggca gtaccgggtc aggccgtctc gcggacgcag cgggccagcg
     5521 tgcgcaggcc ctcgtccagt tcctcggggg tgatggtcag cggcggcagc agcttgacga
     5581 cctcgctctg cgggcccgag gtctccagca ggagccccag ctcgaaggcg cgggcgcaga
     5641 ccgacgaggc gcgcgccggg tcctcgaact ccatgcccca caccaggccc cggccgcgga
     5701 actgcgcggt cggctcctcg gcgcagatgg ccagcagcgt ctgctcgacc tgctcgccgc
     5761 gggccagggt ctgcttttcc atctggccgt ccgcccagta ggcgtccagc gcggcggcgg
     5821 ccgtgacgaa ggccgggttg ttgccgcgga aggtgccgtt gtgctcgccg ggctcccaga
     5881 tgtccagctc cggcttgaac aggcagagcg ccatcggcag tccgtagccg ctgatggact
     5941 tcgacagcgt gacgatgtcc gggacgacgc cggactcctc gaaggagaag aagccgccgg
     6001 tgcggccgca gcccatctgg atgtcgtcga cgatcagcag catgtcctgg cggtcgcaca
     6061 gctcgcgcag cgcgcgcagc cactcgggcc gggcgacgtt gatgccgccc tcgccctgga
     6121 ccgtctccac gatcacggcg gcgggcttgt tgagcccgga gccctggtcc tccagcagtc
     6181 gctcgaacca gaggaagtcg gggaccgtac cgtcgaagta gttgtcgaac ggcatcgggg
     6241 tgccgtgcac cagcgggata ccggccccgg cccgcttgaa cgcgttgccg gtgacggcga
     6301 gcgagcccag cgacatgccg tggaaggcgt tggtgaacga gacgacggac tcgcggccct
     6361 tgaccttgcg ggcgagcttc agcgccgcct cgacggcgtt ggtgccggtc gggccgggga
     6421 acatcacctt gtacggcagg tcgcgcggcc gcaggatcac gttctggaac gtctccagga
     6481 acgcacgctt ggcggtggtc gccatgtcca ggccgtgggt gatgccgtcg cgctcgatgt
     6541 agtcgatcag cgctcgtttc agcaccgggt tgttgtggcc gtagttcagc gagccggccc
     6601 cggcgaagaa gtccaggtag gagtggccgt cctcgtcggt cagccgggcg ccctgggcgc
     6661 ggtcgaacac ggcgggccag ccgcggcagt agctgcggac ctcggactca agagtctcga
     6721 agacgctcag tgcgggcggg gtgatggtca cagcggatct cctgcgtgag ggggtgtgac
     6781 gggcggtggt cgagtgctcc ccgccgctcg gaggcggccg ggaccgcgcc gggtgatcgg
     6841 ccggcgcgaa aagtcttgga ggccgggcgt acgggtcggg tacgccgggg tggtggccgg
     6901 gcgggctcgg ccggcgaacg tgccggtcgg tcggtgagcg tgccgggtca ggcggcgaac
     6961 gggcctatcc ggtagagcac ctcgggcaga tgcgtccctt cggggaacag cgcgccctcg
     7021 aagagcacct cctgctccag cgcgacatcg tggcgctggg cgtaggacgt gaacagccgg
     7081 tcggaggcgg tgttgtccgg ggtgatcgtc gtctccacgg acgacaggcc ctggtcggcg
     7141 gcgacccggg cggtcagcgc gtccagcaga gtggcggcca gccccttgcc gcggtgggcc
     7201 tggtccacgg ccacctgcca gacgacgagg gtccgggggc ggtcgggcct gatgtagccc
     7261 gtcacgaagg cgatgggctc gccgttctcg ccacgggcga cgacggaggt cgcggcgaag
     7321 tcacggcacc acagcaggta gctgtacgag gagttcaggt ccaggacctg ggagtcgcgg
     7381 gcgatgcgcc agatcgcggc tccgtcctcc actcgtgggg tgtcgatact gaggaattcg
     7441 cttcgggcac gggcaaaatc tgctggtgcg gcggtcatgg gaattgaatt tacccagcaa
     7501 atttcgaaat tgcatcgagg tgaagggttg ggtgaagcgc ctccatgtgt tatcaggcgt
     7561 gcgcgagatc caccatgatc cgggtggaat ttcaggctct ttgtcccgaa tttttcgggc
     7621 ataacgccgc ccatgtggag tcggtcacag ggttgtaact ctcctgagac gtgtccgaat
     7681 cacgaagatt cgatgcccgg aaaacttccg cgtttaggtg ccgggaaagc gggcagaaga
     7741 atgcggggag ctgctttcga taaagcagct ccccgcatta tgaatatcag cggcccggcc
     7801 aggtttccga aacggcgacc cgggcagcat cgatatcgac ccgccggccc gcatcggtca
     7861 gcgccgcccc cagagccgcg agcgaggaca gcaccgcgtc ccgcgtcgca tccgccccgt
     7921 agtgattgac ccggatcatc tccttggaca gcgccccgcc gcccgcgacg agcgggagcg
     7981 agggatcggc cgccagcgcg tgcgcgacca gcgcggcggc gtcggtcccc gccggggcgc
     8041 gcagcgtcgt ggccaccgga gcggcgtccc gcgcctcgtg gacgtacggc tccagaccgc
     8101 cgcccagtgc caccgcaccc gccctggtcg ccgcggccgc cgaggcgtgg cgggccatca
     8161 ccgtgcccag gccggcgtcc tcgatccgcg ccacacaggc ctccaggccc agcatctcca
     8221 gctgcgccgg ggcgtgcggc agcgccttgc ggcctccgtc gatccagcgc tccttccagt
     8281 ccaggaggga gaggtaggag cggcgcgggg cggccgggtt ggcggccatc cgctcccagg
     8341 cccgcgcgct caccgacacc gcggagaccc cggcgggccc gcccatggcc ttctgcgcgc
     8401 cgatcacgca cagatcgacg ccccaggcgt ccggcagcag cggttcggcg cccaccgagg
     8461 cgaccgcgtc cagcatgaac agcgcgccgt gcgcccggac cacctcgccg atctccgcga
     8521 ccgggttggt gttgccggtc gccgcctccg cgtgcaccag cgagacgaag tcgatctccg
     8581 ggtgcgcggc gagcgcctcg gcgacctggc cggcggtcac cgccgtgtgg aagggcaccg
     8641 cgaggtcgac gacctgtgcg ccgcagtccc gcagccagtt cccgaaggtc tgcccgtagg
     8701 ggccggtgac cacgttcagc gcggtcgagc cgggccgcgc gccgctgcgg atgcagccct
     8761 ccagcggcag cagcgcctcg ccctgggtga tgacgacgtc ctgctccgtg gcgaggagcc
     8821 gggccacgga ccgctcgatc gccgcgaagc ggtcggcggt cagcggcggg aggtcgagga
     8881 aggggtgcgt cacggtgggg ctctcttcgg gttcgtgcgg cggtcggaaa gacaccggca
     8941 gcctaccgac ggccttccgg cggcctcgta cagtgccgcc tatgagtgat caggaggaac
     9001 agcgggtgct gcgggtgaag ggccgggtgc tcgtcggccc ggacgaggtg cgggacgaac
     9061 tgtgggccgt ggacggacgg atcacctacg agcggccgcc cggcgccgac cgcgcggtga
     9121 cggtcaccgg ctgggcgctg cccggtctgg tcgacgcgca ctgccatgtg ggcctggacg
     9181 cgcacggccc ggtcgacgcg gccaccgccg agaagcaggc gctcaccgac cgggaggccg
     9241 gaacactgct gatccgggac gccggatcgc cctccgacac ccggtgggtc gacgaccgcg
     9301 aggatctgcc gaagatcatc agggccggcc ggcacatcgc caggacccgc cgctacatcc
     9361 gtaactacgc ccatgagatc gagcccggcg acctcgtcgc gtacgtggcc cgggaggccc
     9421 ggcgcgggga cggctgggtc aagctggtgg gagactggat cgaccgcgac gccggggacc
     9481 tgaccgcctg ctggccgcgc ggcgagctgg cggcggccat cgccgaggcg caccggctcg
     9541 gcgcccgggt caccgcgcac tgcttcgccg aggagtcgct caccgacctg gtcgaggcgg
     9601 gtatcgactg catcgagcac gccaccggcc tcaccgagga gaccatcccg ctcttcgccg
     9661 aacgcggggt cgcgatcgtc ccgacgctgg tcaacatcgc caccttcccg gacctcgcgg
     9721 cgggcggcga ggccaagttc ccgcgctggt cggcccatat gcggcagctg tacgaacgcc
     9781 gctacgacac cgtgcgcgcc gcgtacgacg cgggcatccc ggtctacgtc ggcaccgacg
     9841 cgggcggctc gctggcgcac ggcctggtcg cgggcgaggt ggccgagctg gtcaaggcgg
     9901 gcatcccgcc gctggaggcc ctctccgcca ccgcgtgggg agccaggcgc tggctggggc
     9961 ggccggtgct ggaggagggc gccccggcgg acctggtggt gtacgaggag gatccgcgcg
    10021 cggacgtccg ggtgctggcg gctcctcggc acatcgtgct gaacgggcgt gtgatcggct
    10081 gagggcggcg cggtgccgtg ttgacgggca gtgacggggg cggccccggc tcgttgaagc
    10141 accttcgcgc atgttgcggt gtcgtccggt gtgttcgggt gccgtacgac cggagccggg
    10201 tgaaacgagt gacgccgggg aacgcgcgtg ccgaaagatt cgaatatgcg agcggaaacc
    10261 cccctttggg gtgaactcac gccaggtatc cgccagttca ccctcagtgc gtaaagattc
    10321 acggagtcga ggccgccggc acgggcgatg tcccccattg gactgtgccg gtggctccat
    10381 gtctcttgtg ggggttcca
//
