LOCUS       NZ_JALDPQ010000049      4284 bp    DNA     linear   CON 28-MAY-2024
DEFINITION  Streptomyces sp. TRM75563 703, whole genome shotgun sequence.
ACCESSION   NZ_JALDPQ010000049
VERSION     NZ_JALDPQ010000049.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. TRM75563
  ORGANISM  Streptomyces sp. TRM75563
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 4284)
  AUTHORS   Chen,Y.
  TITLE     Streptomyces aheensis sp. nov., isolated from road
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4284)
  AUTHORS   Chen,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2022) College of Life Science, Tarim University,
            Hongqiao, Alar 843300, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 14-MAR-2022
            Assembly Method                   :: ABySS v. V2.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 400.0x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022748615.1-RS_2024_05_28
            Annotation Date                   :: 05/28/2024 05:18:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,476
            CDSs (total)                      :: 7,413
            Genes (coding)                    :: 7,165
            CDSs (with protein)               :: 7,165
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 56
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 249
            CDSs (without protein)            :: 248
            Pseudo Genes (ambiguous residues) :: 1 of 249
            Pseudo Genes (frameshifted)       :: 108 of 249
            Pseudo Genes (incomplete)         :: 202 of 249
            Pseudo Genes (internal stop)      :: 19 of 249
            Pseudo Genes (multiple problems)  :: 75 of 249
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 19:22:52
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 4284
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JALDPQ010000049.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     cand_cluster    1..4284
                     /SMILES="C=CC(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(PKS_AT and (PKS_KS or ene_KS or
                     mod_KS or hyb_KS or itr_KS or tra_KS))"
                     /kind="single"
                     /product="T1PKS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..4284
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[<0:4262](-)"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1..4262)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
     aSDomain        complement(<1..767)
                     /ASF="found active site cysteine: True, scaffold matched
                     GSSS: True"
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MQC87_RS09300_PKS_KS.1"
                     /domain_subtypes="Modular-KS"
                     /evalue="2.50E-106"
                     /label="MQC87_RS09300_PKS_KS.1"
                     /locus_tag="MQC87_RS09300"
                     /protein_end="1421"
                     /protein_start="1165"
                     /score="348.0"
                     /tool="antismash"
                     /translation="VVIVGMACRYPGDVRSPDDLWQLVATGTDAIGPFPENRGWDVENL
                     YDPDPDKAGKSYTRHGGFLYDADRFDAEFFGISPREAAGMDPQQRLLLETSWEAVENAG
                     IAPTALHGTRTGVFCGVMYSDYTSRLPATPQSVEAYGFTGNSPSVVSGRVSYTLGLKGP
                     AITVDTACSSSLVATHLAAQALRNGECELALAGGVTVMSAPSTFIEFSRQRGLATDGRC
                     KAFSDSADGTAWSEGVGVLLLERLSDARRNGHEV"
     gene            complement(<1..4262)
                     /locus_tag="MQC87_RS09300"
                     /old_locus_tag="MQC87_09300"
     CDS             complement(<1..4262)
                     /NRPS_PKS="Domain: PKS_AT (0-221). E-value: 4.8e-86. Score:
                     280.7. Matches aSDomain:
                     nrpspksdomains_MQC87_RS09300_PKS_AT.1"
                     /NRPS_PKS="Domain: PKS_DH (294-462). E-value: 2.6e-55.
                     Score: 179.3. Matches aSDomain:
                     nrpspksdomains_MQC87_RS09300_PKS_DH.1"
                     /NRPS_PKS="Domain: PKS_KR (786-961). E-value: 5.8e-59.
                     Score: 191.0. Matches aSDomain:
                     nrpspksdomains_MQC87_RS09300_PKS_KR.1"
                     /NRPS_PKS="Domain: ACP (1068-1140). E-value: 2.6e-24.
                     Score: 77.3. Matches aSDomain:
                     nrpspksdomains_MQC87_RS09300_ACP.1"
                     /NRPS_PKS="Domain: PKS_KS(Modular-KS) (1165-1421). E-value:
                     2.5e-106. Score: 348.0. Matches aSDomain:
                     nrpspksdomains_MQC87_RS09300_PKS_KS.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     PKS_AT"
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     mod_KS"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ketoacyl-synt"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1021: malonyl CoA-acyl carrier protein transacylase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /locus_tag="MQC87_RS09300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MQC87_09300"
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_242737990.1"
                     /sec_met_domain="PKS_AT (E-value: 8e-88, bitscore: 280.7,
                     seeds: 1682, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short (E-value: 2.9e-63, bitscore:
                     199.1, seeds: 230, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 3.1e-14, bitscore:
                     39.6, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="mod_KS (E-value: 5.4e-135, bitscore:
                     436.5, seeds: 217, tool: rule-based-clusters)"
                     /sec_met_domain="ketoacyl-synt (E-value: 3.3e-98, bitscore:
                     314.3, seeds: 77, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEVALFRLLEHYGVTPDRLLGHSVGEIAAAHAAGVLDLTDACTLV
                     AHRGRLMQTAPTGGTMTAIEATEDEIRTSLTPYTGRLDLAAVNGPTATVITGDTDAATE
                     LTTLWKNKGRRTTDLKVSHAFHSPHMDPILNDFRTITTTLTYHPPRIPLISNITGKTAT
                     TEELTDPEYWVRQLRTTVRFADGIHTLQDDRFTTYVELGPAPVLSAMARDCLGEQGPRP
                     ITLLRPDRSESHTFTTAVAQLALHGFAPDPQLLFPGARRTALPTYAFQRRRYWLDTPVP
                     AEHTAGLGLTPAGHPLLGSFTSLAADGGLLLAGHLSPHTHPWLAQHVIGGSALLPGAAV
                     VELAVTAGDRADCGGLRELVLEEPLFVPDEGVRIQITVGPADATGACSVAVHSRRDTPA
                     APGARDEGEWTRHASGVLAPTGGTTAADDGAWPPPGARPLTHEGLYKRLTDLGYAYGPA
                     FQGLRSVWQSDTELYAEVTLPEELHGEAADYGIHPALLDAAQHALLIGLLPVADEDFGD
                     LRLPFSYDGVTLHASGATRLRVRLTRGEGDSVTLTATDPAGQPVLSVDSLILRRVPAGR
                     IATGNGPRDGLHRLAWQPVPAPGPASDAHSAWAVLGARAEGLDARAEGLDAVPYQDLSA
                     LERALSAGEPAPDVLVLPCHTPDELLTEVSTTRTAVREMLAVVRRCLADVRLQSTTFLL
                     LTRGAVAAVDGEDVHDLPAAAVHGLLRTAASEHPGRFALLDTDGDDLARIAEAASSAAA
                     GSLQLALRDGVLLAPRLTRTPGPQRTEAAFDPEGTVLVTGGTGGLGSLVARHLAGRHGV
                     RHLLLCSRSGRAPGLVAELAELGAAATVVACDVTDRERLAQVLAAVPQEHPLTAVVHTA
                     GVLADATLDNLGPDAVDRVLGAKADGARHLHELTARMPLDAFVVFSSVAGLLGTPGQGA
                     YAAANAHIDALAQHRRAHGLPATSVAWGLWDPAAGGMAAELSGADIARWGRNGILPLTA
                     ERGMELFDAALATGEALLVAAELDPAALRAPAATVPTLLRSLASPARRRVQATAVEGDA
                     GSWAARTSGLPEAERRRSVAELVRTTVSSVLALPQASTLAEDAAFRDLGLDSLTGLELR
                     GRLAAVSEVKLAATVVFDHPTPAALTEHLMAQLDRTSSSEQHSSRGAGAPAPAVVDDPV
                     VIVGMACRYPGDVRSPDDLWQLVATGTDAIGPFPENRGWDVENLYDPDPDKAGKSYTRH
                     GGFLYDADRFDAEFFGISPREAAGMDPQQRLLLETSWEAVENAGIAPTALHGTRTGVFC
                     GVMYSDYTSRLPATPQSVEAYGFTGNSPSVVSGRVSYTLGLKGPAITVDTACSSSLVAT
                     HLAAQALRNGECELALAGGVTVMSAPSTFIEFSRQRGLATDGRCKAFSDSADGTAWSEG
                     VGVLLLERLSDARRNGHEV"
     source          1..4284
                     /collection_date="2020-03-14"
                     /country="China"
                     /db_xref="taxon:2817418"
                     /isolation_source="aheensis"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces sp. TRM75563"
                     /strain="TRM75563"
     region          1..4284
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="T1PKS"
                     /region_number="1"
                     /rules="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or
                     hyb_KS or itr_KS or tra_KS))"
                     /tool="antismash"
     CDS_motif       complement(237..284)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MQC87_RS09300_0010"
                     /evalue="7.10E-07"
                     /label="PKSI-KS_m3"
                     /locus_tag="MQC87_RS09300"
                     /protein_end="1342"
                     /protein_start="1326"
                     /score="21.0"
                     /tool="antismash"
                     /translation="GPAITVDTACSSSLVA"
     CDS_motif       complement(312..335)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MQC87_RS09300_0009"
                     /evalue="9.90E+01"
                     /label="PKSI-AT-M_m5"
                     /locus_tag="MQC87_RS09300"
                     /protein_end="1317"
                     /protein_start="1309"
                     /score="-2.9"
                     /tool="antismash"
                     /translation="NSPSVVSG"
     aSDomain        complement(843..1058)
                     /ASF="found active site serine: True"
                     /ASF="non-beta-branching ACP"
                     /aSDomain="ACP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MQC87_RS09300_ACP.1"
                     /evalue="2.60E-24"
                     /label="MQC87_RS09300_ACP.1"
                     /locus_tag="MQC87_RS09300"
                     /protein_end="1140"
                     /protein_start="1068"
                     /score="77.3"
                     /tool="antismash"
                     /translation="ELVRTTVSSVLALPQASTLAEDAAFRDLGLDSLTGLELRGRLAAV
                     SEVKLAATVVFDHPTPAALTEHLMAQL"
     aSModule        complement(843..4262)
                     /complete
                     /domains="nrpspksdomains_MQC87_RS09300_PKS_AT.1"
                     /domains="nrpspksdomains_MQC87_RS09300_PKS_DH.1"
                     /domains="nrpspksdomains_MQC87_RS09300_PKS_KR.1"
                     /domains="nrpspksdomains_MQC87_RS09300_ACP.1"
                     /locus_tags="MQC87_RS09300"
                     /monomer_pairings="mal -> ccmal"
                     /starter_module
                     /tool="antismash"
                     /type="pks"
     aSDomain        complement(1380..1904)
                     /ASF="KR domain putatively catalyzing D-configuration
                     product formation"
                     /ASF="catalytic triad S,Y,N inconclusive"
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MQC87_RS09300_PKS_KR.1"
                     /evalue="5.80E-59"
                     /label="MQC87_RS09300_PKS_KR.1"
                     /locus_tag="MQC87_RS09300"
                     /protein_end="961"
                     /protein_start="786"
                     /score="191.0"
                     /specificity="KR activity: active"
                     /specificity="KR stereochemistry: (unknown)"
                     /tool="antismash"
                     /translation="GTVLVTGGTGGLGSLVARHLAGRHGVRHLLLCSRSGRAPGLVAEL
                     AELGAAATVVACDVTDRERLAQVLAAVPQEHPLTAVVHTAGVLADATLDNLGPDAVDRV
                     LGAKADGARHLHELTARMPLDAFVVFSSVAGLLGTPGQGAYAAANAHIDALAQHRRAHG
                     LPATSVAWGLWD"
     CDS_motif       complement(1425..1535)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MQC87_RS09300_0008"
                     /evalue="5.40E-20"
                     /label="PKSI-KR_m4"
                     /locus_tag="MQC87_RS09300"
                     /protein_end="946"
                     /protein_start="909"
                     /score="64.0"
                     /tool="antismash"
                     /translation="LDAFVVFSSVAGLLGTPGQGAYAAANAHIDALAQHRR"
     CDS_motif       complement(1800..1901)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MQC87_RS09300_0007"
                     /evalue="1.80E-10"
                     /label="PKSI-KR_m1"
                     /locus_tag="MQC87_RS09300"
                     /protein_end="821"
                     /protein_start="787"
                     /score="33.4"
                     /tool="antismash"
                     /translation="TVLVTGGTGGLGSLVARHLAGRHGVRHLLLCSRS"
     CDS_motif       complement(2100..2159)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MQC87_RS09300_0006"
                     /evalue="5.70E+01"
                     /label="PKSI-AT-mM_m3"
                     /locus_tag="MQC87_RS09300"
                     /protein_end="721"
                     /protein_start="701"
                     /score="-2.9"
                     /tool="antismash"
                     /translation="AVDGEDVHDLPAAAVHGLLR"
     aSDomain        complement(2877..3380)
                     /ASF="catalytic triad H,G,P inconclusive"
                     /aSDomain="PKS_DH"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MQC87_RS09300_PKS_DH.1"
                     /evalue="2.60E-55"
                     /label="MQC87_RS09300_PKS_DH.1"
                     /locus_tag="MQC87_RS09300"
                     /protein_end="462"
                     /protein_start="294"
                     /score="179.3"
                     /tool="antismash"
                     /translation="HPLLGSFTSLAADGGLLLAGHLSPHTHPWLAQHVIGGSALLPGAA
                     VVELAVTAGDRADCGGLRELVLEEPLFVPDEGVRIQITVGPADATGACSVAVHSRRDTP
                     AAPGARDEGEWTRHASGVLAPTGGTTAADDGAWPPPGARPLTHEGLYKRLTDLGYAYGP
                     AFQGL"
     CDS_motif       complement(3222..3296)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MQC87_RS09300_0005"
                     /evalue="1.70E-08"
                     /label="PKSI-DH_m2"
                     /locus_tag="MQC87_RS09300"
                     /protein_end="347"
                     /protein_start="322"
                     /score="26.9"
                     /tool="antismash"
                     /translation="WLAQHVIGGSALLPGAAVVELAVTA"
     aSDomain        complement(3600..4262)
                     /ASF="Malonyl-CoA specific"
                     /ASF="found active site serine: True, scaffold matched
                     GHGE: True"
                     /aSDomain="PKS_AT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MQC87_RS09300_PKS_AT.1"
                     /evalue="4.80E-86"
                     /label="MQC87_RS09300_PKS_AT.1"
                     /locus_tag="MQC87_RS09300"
                     /protein_end="221"
                     /protein_start="0"
                     /score="280.7"
                     /specificity="substrate consensus: mal"
                     /specificity="PKS signature: Malonyl-CoA"
                     /specificity="Minowa: mal"
                     /tool="antismash"
                     /translation="MEVALFRLLEHYGVTPDRLLGHSVGEIAAAHAAGVLDLTDACTLV
                     AHRGRLMQTAPTGGTMTAIEATEDEIRTSLTPYTGRLDLAAVNGPTATVITGDTDAATE
                     LTTLWKNKGRRTTDLKVSHAFHSPHMDPILNDFRTITTTLTYHPPRIPLISNITGKTAT
                     TEELTDPEYWVRQLRTTVRFADGIHTLQDDRFTTYVELGPAPVLSAMARDCLGEQGPR"
     CDS_motif       complement(3714..3749)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MQC87_RS09300_0004"
                     /evalue="4.90E-04"
                     /label="PKSI-AT-M_m8"
                     /locus_tag="MQC87_RS09300"
                     /protein_end="183"
                     /protein_start="171"
                     /score="13.6"
                     /tool="antismash"
                     /translation="YWVRQLRTTVRF"
     CDS_motif       complement(3786..3818)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MQC87_RS09300_0003"
                     /evalue="2.90E-03"
                     /label="PKSI-AT-M_m7"
                     /locus_tag="MQC87_RS09300"
                     /protein_end="159"
                     /protein_start="148"
                     /score="10.4"
                     /tool="antismash"
                     /translation="PRIPLISNITG"
     CDS_motif       complement(3972..4010)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MQC87_RS09300_0002"
                     /evalue="6.10E-03"
                     /label="PKSI-AT-M_m5"
                     /locus_tag="MQC87_RS09300"
                     /protein_end="97"
                     /protein_start="84"
                     /score="10.0"
                     /tool="antismash"
                     /translation="AAVNGPTATVITG"
     CDS_motif       complement(4104..4226)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MQC87_RS09300_0001"
                     /evalue="6.30E-22"
                     /label="PKSI-AT-M_m3"
                     /locus_tag="MQC87_RS09300"
                     /protein_end="53"
                     /protein_start="12"
                     /score="69.8"
                     /tool="antismash"
                     /translation="GVTPDRLLGHSVGEIAAAHAAGVLDLTDACTLVAHRGRLMQ"
ORIGIN
        1 acctcgtggc cgttgcgacg cgcgtcggag agccgctcca gaagcagcac acccacaccc
       61 tcgctccagg cggtaccgtc cgcggagtcc gagaacgcct tgcagcggcc gtccgtggcg
      121 aggccgcgct ggcggctgaa ctcgatgaac gtgctgggtg cggacatgac cgtgacgccg
      181 ccggccaacg cgagctcgca ctcgccgttg cgcagcgcct gcgcggccag gtgcgtggcg
      241 acgagagacg aggagcacgc cgtgtccacc gtgattgcag gccctttgag accgagggtg
      301 tacgagacac gaccggagac cacgctcggt gagttgcctg tgaacccgta ggcctcgacg
      361 ctctgcgggg tggcgggcag ccgcgaggtg tagtcgctgt acatgacgcc gcagaacacg
      421 ccggtgcggg tgccgtgaag tgccgtgggc gcgatgcccg cattctccac ggcctcccag
      481 ctggtctcca gcaggagccg ctgctgaggg tccattcccg cggcctcacg agggctgatg
      541 ccgaagaact cggcatcgaa gcggtccgcg tcgtagagga agccgccgtg ccgtgtgtac
      601 gacttcccgg ccttgtcggg atccgggtcg tagaggttct cgacatccca gccccggttc
      661 tccgggaagg ggccgatggc gtcggtgccg gtggcgacga gctgccacag gtcgtcgggg
      721 gaacggacgt cgcccgggta gcggcaggcc atccccacga tcacgacggg gtcgtccaca
      781 accgcggggg ccggcgcccc tgctccccgg gacgagtgct gctcggagga ggacgttcgg
      841 tcgagctgcg ccatgaggtg ctcggtgagg gccgcgggag tgggatggtc gaagaccacc
      901 gtggcggcca gcttgacttc cgagaccgcg gcgagacggc cgcgcagttc cagaccggtc
      961 agggagtcga ggcccaggtc gcggaaggcc gcgtcctcgg cgagggtcga ggcctgcggc
     1021 agcgcgagta ccgaggagac cgtcgtgcgg accaactccg ctaccgaacg acggcgttcg
     1081 gcctcgggca gtccggaggt ccgcgctgcc caggagccgg cgtcgccctc gacggccgtg
     1141 gcctggaccc ggcgtcgggc aggggacgcg agtgagcgca gcagcgtggg cacggtcgcg
     1201 gcgggggcgc gcagcgcggc cgggtccagt tcggccgcga cgagcagcgc ctcgcccgtg
     1261 gccagggccg cgtcgaacag ctccatgccg cgctccgcgg tcagcggcag gatgccgttg
     1321 cggccccatc gggcgatgtc ggcaccggag agttctgcgg ccatgccgcc tgccgcgggg
     1381 tcccacagcc cccaggcgac cgatgtcgcg ggcagtccat gggccctgcg atgctgtgcc
     1441 agggcgtcga tgtgggcgtt ggccgcggcg taggcgccct ggccgggggt gccgaggagt
     1501 cccgcgaccg aggagaacac cacgaacgcg tccaacggca tccgcgcggt gagctcgtgc
     1561 aggtggcggg cgccgtcggc cttggcgccg agcacccggt cgacggcgtc ggggccgaga
     1621 ttgtccaggg tggcgtcggc caggacaccc gcggtgtgca ccacggcggt cagcgggtgt
     1681 tcctgcggta cggcggccag cacctgggcg agacgttcgc ggtcggtgac gtcgcatgcc
     1741 acgaccgtcg ctgcggcgcc cagttcggcc agttcggcga ccaggccggg tgcccggccg
     1801 ctacggctgc acagcagcag gtggcgcacg ccgtgccggc ccgccaggtg gcgggctacc
     1861 aggctgccca ggccaccggt gccgccggtg accaggacgg tgccctccgg gtcgaatgcc
     1921 gcctcggtgc gctgcggacc cggggtgcgg gtaaggcggg gcgccaacag gacgccgtcc
     1981 cgcagcgcca gttggaggga tcccgcagcg gcggaggagg ccgcttcggc tatgcgggcc
     2041 aggtcgtcgc cgtccgtgtc gaggagcgcg aagcggcccg ggtgctcgct cgccgctgtg
     2101 cgcagcagcc cgtgcacggc cgcggcgggc agatcgtgga cgtcctcgcc gtccacggca
     2161 gcgaccgcac cacgggtgag gagcaggaag gtcgtcgact gcaggcgtac gtcggcgagg
     2221 cagcgtcgca cgacggcgag catctcgcgg accgctgtgc gggtcgtcga cacctcggtg
     2281 aggagttcgt ccggagtgtg gcagggcagc accaggacat cgggggccgg ctcgccggcg
     2341 gagagggccc tctccagggc ggagaggtcc tggtacggga cggcgtcgag cccttcggca
     2401 cgggcgtcga gcccttcggc acgggcgccg agcacggccc aggcgctgtg ggcgtcggag
     2461 gcgggcccgg gggcaggaac cggctgccat gccaggcggt gcagcccgtc gcgaggtccg
     2521 ttgccggtgg cgatgcggcc tgccggtacc cggcgcagga tcagggagtc gacggacagc
     2581 acgggctgcc cggcggggtc cgtggcggtc aaggtgacgc tgtcgccctc gccccgggtc
     2641 agacgtactc gcagtcgcgt ggcccctgag gcgtgcaggg tgacgccgtc gtacgagaac
     2701 ggcaggcgca ggtcaccgaa gtcctcgtcg gccacgggca gcagtccgat caggagcgcg
     2761 tgctgcgcgg cgtccaggag cgcgggatgg atgccgtaat cggctgcctc cccgtgcagt
     2821 tcctcgggca gcgtcacctc cgcgtacagc tccgtgtccg actgccagac ggagcggagc
     2881 ccttggaagg ccgggccgta ggcgtagccc agatcggtca gccgcttgta caggccctcg
     2941 tgcgtgaggg ggcgtgcacc gggcgggggc caggcgccgt cgtctgcggc cgtcgtaccg
     3001 ccggtcgggg cgaggacgcc tgaggcgtgc cgggtccact cgccctcgtc ccgggctccg
     3061 ggggctgccg gcgtgtcccg gcgtgagtga acggcgaccg agcatgctcc ggtcgcatcg
     3121 gcggggccca cggtgatctg aatccggacg ccctcgtccg gcacgaacag cggttcttcc
     3181 aggaccagtt cgcgtagacc gccacagtcg gcccggtcgc ccgcggtcac tgccagctcg
     3241 actacggccg cgccgggcag cagcgcggat ccgccgatca cgtgctgggc gagccaggga
     3301 tgcgtgtgcg gtgagaggtg tccggcgagc agcagcccgc cgtctgcggc gaggctcgtg
     3361 aagctgccga gcagcgggtg acccgcgggc gtgagaccga ggcccgcggt gtgttccgcc
     3421 gggacggggg tgtccagcca gtagcgccgc cgctggaagg cgtaggtggg aagtgcggtc
     3481 cgccgggcgc cggggaagag cagctgtgga tccggggcga agccgtgcag tgcgagctgc
     3541 gcgacggccg tcgtgaaggt gtgcgactcg gaccggtcgg gacgcaggag cgtgatcggc
     3601 cgcggcccct gttcgccgag gcagtcccgg gccatggccg agaggacggg cgccgggccc
     3661 agctccacgt acgtggtgaa ccggtcgtcc tggagggtgt ggattccgtc ggcgaagcgg
     3721 acggtggtgc ggagttgtcg gacccagtat tcggggtcgg tgagttcttc ggtggtggcg
     3781 gttttgccgg tgatgttgga gatgaggggg atgcgtgggg ggtggtaggt gagtgtggtg
     3841 gtgatggtgc ggaagtcgtt gaggatgggg tccatgtggg gtgagtggaa ggcgtggctg
     3901 actttgaggt cggtggttcg gcggcctttg tttttccaca gggtggtgag ttcggtggcg
     3961 gcgtcggtgt cgccggtgat gacggtggcg gtggggccgt tgacggcggc gaggtcgagg
     4021 cgtccggtgt agggggtgag ggaggtgcgg atttcgtctt cggtggcttc gatggctgtc
     4081 atggtgccgc cggtgggggc ggtttgcatg aggcggcctc ggtgggcgac gagggtgcag
     4141 gcgtcggtga ggtcgaggac tcctgctgcg tgggcggcgg cgatttcgcc gacggagtgg
     4201 ccgaggagtc ggtcgggtgt gacgccgtag tgttcgagga ggcggaagag ggcgacctcc
     4261 accgcgaaca gcgcgggctg cgtg
//
