LOCUS       NZ_CP074380            10405 bp    DNA     linear   CON 27-MAY-2024
DEFINITION  Streptomyces sp. MST-110588 chromosome, complete genome.
ACCESSION   NZ_CP074380
VERSION     NZ_CP074380.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. MST-110588
  ORGANISM  Streptomyces sp. MST-110588
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7915503)
  AUTHORS   Booth,T.J., Bozhueyuek,K.A.J., Liston,J.D., Lacey,E. and
            Wilkinson,B.
  TITLE     Bifurcation drives the evolution of assembly-line biosynthesis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7915503)
  AUTHORS   Booth,T.J., Bozhueyuek,K.A.J., Liston,J.D., Lacey,E. and
            Wilkinson,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAY-2021) Molecular Microbiology, John Innes Centre,
            John Innes Centre, Norwich, Not US or Canada NR4, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 28-FEB-2016
            Assembly Method                   :: HGAP v. 3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 25.0x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022695595.1-RS_2024_05_27
            Annotation Date                   :: 05/27/2024 05:38:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,918
            CDSs (total)                      :: 6,828
            Genes (coding)                    :: 6,327
            CDSs (with protein)               :: 6,327
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 501
            CDSs (without protein)            :: 501
            Pseudo Genes (ambiguous residues) :: 0 of 501
            Pseudo Genes (frameshifted)       :: 350 of 501
            Pseudo Genes (incomplete)         :: 185 of 501
            Pseudo Genes (internal stop)      :: 14 of 501
            Pseudo Genes (multiple problems)  :: 45 of 501
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 12:56:23
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6551281
            Orig. end                         :: 6561686
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP074380.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Barrie Wilkinson, John Innes
            Centre, Norwich.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10405
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="26"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10405
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10405
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5405](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5405)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    542..557
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 19.45"
                     /tool="antismash"
     gene            832..2694
                     /locus_tag="KGS77_RS35095"
     CDS             832..2694
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_function="GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /locus_tag="KGS77_RS35095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="WP_347404540.1"
                     /transl_table=11
                     /translation="MAPHIHEVVPVDGTPRYPPRDEESHRRLVYGEESALGEAYDAFGA
                     LVRAVARRVTGSAHSADEVVQEVFLRLWTCPYAFAPGHGSLRAWLSMLAHHRAVLRMRD
                     AAGEPGEPGESEEAGEAGEEAGEAGRRARDAEAALMLHSALAELPLPQREAMHLACFAG
                     RTCRQVAVELGIPEEIAASRLRTALGGLADRLTALPGHVPADPRTSQSPQESQETQELQ
                     GAQELPQASPPGPGDPESSAEDDHQAVSELLAAWVLGAGMPGDDARVRRHLRSCAPCAA
                     EARRLGEAVRLLDEPAESGATEAKGARQVKGVKGAGSRKGGGKGAVRTVRKGNTGVARG
                     RLMAAARTARPGHGPLAAHAAPYAGAVASLDALLHELDTAPALWGTPVVHDWDVQGTLA
                     HLVAADEALAERLGLAPVTMTGGSQGPARVAMPGGPWELRWSARTEEVVAYEHTRPTAE
                     TREAWRTQAHGLLALPEAGDEQLAASPTVLMGLRLPVADHYVLRALETWVHTRDIGRAL
                     GRPVPPPLPTHLQRFVGLSIRILDRALGPTARPVLLSVEGEAGGDWVLGSDAEPIAAEL
                     VLEAVDFCLLVGGRRIPAEIARGHAGDPVASQRVLETAASLAWL"
     misc_feature    2772..2785
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 17.76"
                     /tool="antismash"
     misc_feature    2776..2790
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 17.07"
                     /tool="antismash"
     gene            2900..3937
                     /locus_tag="KGS77_RS28720"
                     /old_locus_tag="KGS77_28515"
     CDS             2900..3937
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF016235.4"
                     /locus_tag="KGS77_RS28720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_28515"
                     /product="DUF475 domain-containing protein"
                     /protein_id="WP_242586055.1"
                     /transl_table=11
                     /translation="MACFALVGAAVALLGGWAHLVPLVPIMLLGFALGVNEAIASVGPL
                     KRIPGFWQRFFLLPGVILAAVIGRLGVPFFVVTLSSGEPPGQVLSLALHEPHRYEEMLA
                     AAEPAVYAFSGTFLLLAFFEFIFIDREVKWLAWLERPLGRAGGMPNLGLMLAMTVLLCT
                     AWFIGERPAAILTAGLLALLSYVVIHSIAAFLRERARPGSPSNGESRAVDAGTAGVAIT
                     LSSIEVADIAVGAEASAAAFIFDKNIFVIAAGLGMAAFVARSSVLLWTRRQTLRTWAYA
                     RHGAAYGTGAIAALLLLKTGYAVPTYVNVLVVAVMLGAALLSSHRLEKTTVEQAASEAE
                     GQRTR"
     gene            complement(4012..4911)
                     /gene="thpD"
                     /locus_tag="KGS77_RS28725"
                     /old_locus_tag="KGS77_28520"
     CDS             complement(4012..4911)
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006606339.1"
                     /locus_tag="KGS77_RS28725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_28520"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_242586056.1"
                     /sec_met_domain="PhyH (E-value: 5.8e-37, bitscore: 126.6,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTAPERTADLYPTRGATEVITPRQDPVVWSQPGTAGPFRPSELE
                     DFARDGFFAIDELITADEVAVYRAELDRLVNDSRMRADDRSIVEPKSQDIRSVFEVHKI
                     SEVFAGLVADPRVVGRARQILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLP
                     RMRTVSISIALTENHDTNGSLMIMPGSHRHFLGCAGETPRDNYKKSLQMQDAGTPSDRA
                     LTEMADRHGIRLFTGKPGSATWFDCNAMHGSGDNITPYPRSNVFIVFNSVENTAVEPFA
                     APVRRPEYIGARDFTPVR"
     gene            complement(5001..5405)
                     /locus_tag="KGS77_RS28730"
                     /old_locus_tag="KGS77_28525"
     CDS             complement(5001..5405)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009718913.1"
                     /locus_tag="KGS77_RS28730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_28525"
                     /product="ectoine synthase"
                     /protein_id="WP_242586057.1"
                     /sec_met_domain="ectoine_synt (E-value: 1.5e-53, bitscore:
                     178.6, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFKDIEGTDRHVKAKSGTWESKRIVLAKERVGFSLHETTLY
                     AGTETSMWYANHIEAVLCVEGEAELTNDETGEKHWITPGTMYLLDGHERHTMRPKTDFR
                     CVCVFNPPVTGREDHDENGVYPLLTEPDAA"
     misc_feature    complement(5433..5446)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 14.41"
                     /tool="antismash"
     misc_feature    complement(5442..5455)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 14.41"
                     /tool="antismash"
     gene            complement(5497..6765)
                     /gene="ectB"
                     /locus_tag="KGS77_RS28735"
                     /old_locus_tag="KGS77_28530"
     CDS             complement(5497..6765)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-365). E-value: 6.6e-73.
                     Score: 237.6. Matches aSDomain:
                     nrpspksdomains_KGS77_RS28735_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448159.1"
                     /locus_tag="KGS77_RS28735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_28530"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_242586058.1"
                     /sec_met_domain="Aminotran_3 (E-value: 3.7e-75, bitscore:
                     251.7, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITQPDLSVFETVESEVRSYCRGWPTVFDRAQGSRMYDEDGHTY
                     LDFFAGAGSLNYGHNNPVLKRALIDYIERDGVTHGLDMSTTAKRAFLESFQNIVLRPRD
                     LPYKVMFPGPTGTNAVEAALKLARKVKGRESIVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDNYLDGTYPDFLWFERLLEDQGSGLNRPAAVIVETVQGEGGINVARP
                     EWLRALADLCERRDMLLIVDDIQMGCGRTGAFFSFEEAGIVPDIVTVSKSISGYGLPLA
                     LTLFKPELDVWEPGEHNGTFRGNNPAFVTAAAALDTYWADGEMEKQTLGRGEIVESHLR
                     AIVAAHPELGGEYRGRGLVWGLEFPDKELAGKVARRAFELGLLIETSGPQGEVVKLLPA
                     LTTTPDELDEGLRTLARAVRDCA"
     aSDomain        complement(5671..6681)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_KGS77_RS28735_Aminotran_3.1"
                     /evalue="6.60E-73"
                     /label="KGS77_RS28735_Aminotran_3.1"
                     /locus_tag="KGS77_RS28735"
                     /protein_end="365"
                     /protein_start="28"
                     /score="237.6"
                     /tool="antismash"
                     /translation="FDRAQGSRMYDEDGHTYLDFFAGAGSLNYGHNNPVLKRALIDYIE
                     RDGVTHGLDMSTTAKRAFLESFQNIVLRPRDLPYKVMFPGPTGTNAVEAALKLARKVKG
                     RESIVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDNYLDGTYPDFLWFER
                     LLEDQGSGLNRPAAVIVETVQGEGGINVARPEWLRALADLCERRDMLLIVDDIQMGCGR
                     TGAFFSFEEAGIVPDIVTVSKSISGYGLPLALTLFKPELDVWEPGEHNGTFRGNNPAFV
                     TAAAALDTYWADGEMEKQTLGRGEIVESHLRAIVAAHPELGGEYRGRGLVWGLEFP"
     gene            complement(6923..7507)
                     /gene="ectA"
                     /locus_tag="KGS77_RS28740"
                     /old_locus_tag="KGS77_28535"
     CDS             complement(6923..7507)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006123622.1"
                     /locus_tag="KGS77_RS28740"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_28535"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_242586059.1"
                     /transl_table=11
                     /translation="MTAAPADRARSRSDFSDISAIREMPEGFKLDTPRVEDGAAIWRIA
                     RDSKTLDLNSSYSYLLWCRDFAATSAVARDARGEPAGFVTGYLRPERPGTLVVWQLAVD
                     HAHRGRGLAAALVDGLAERAAREYGIRYLETTVTPGNTASERVFTSFAERHAGGHRREV
                     LFDAGLFPEQGHEPEVLHLIGPLDTLAAHRP"
     misc_feature    complement(7608..7622)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.55"
                     /tool="antismash"
     misc_feature    complement(7629..7643)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 17.95"
                     /tool="antismash"
     misc_feature    complement(7664..7679)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     gene            8025..9116
                     /locus_tag="KGS77_RS28745"
                     /old_locus_tag="KGS77_28540"
     CDS             8025..9116
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006606343.1"
                     /locus_tag="KGS77_RS28745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_28540"
                     /product="amidohydrolase family protein"
                     /protein_id="WP_242586060.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 1.2e-18, bitscore:
                     66.1, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSESVVLHIKGRVLAGPQDVRDELWVVDGRVTYERPGGAREITTV
                     RGWALPGLVDAHCHVGLDAHGPVDEATSEKQALTDRDAGTLLIRDAGSPSDTRWIDDRE
                     DLPRIIRAGRHIARTRRYIRNYAHEIEPEDLVAYVAREARRGDGWVKLVGDWIDRESGD
                     LAACWPRGEVEAAIAEAHRLGARVTAHCFAEESLAPLVEAGIDCIEHATGLTEETIPLF
                     AERGVAIVPTLINIATFPTLAAAGEGKFPRWADHMRRLHERRYDTVRAAYDAGVPVFAG
                     TDAGGSLAHGLVAQEVAELMKAGIPAVDALSATTWSARTWLGRPGLTEGAPADLVVYDS
                     DPREDVRVLAAPRRVVLRGRVVG"
ORIGIN
        1 cttgcgcgcc acgcgtgggt acgccgcccg gtagacgtcc tccgcgccgg tgtccagccc
       61 cggcaggccg ccggtgacga acacctcgcg cagcccctcc ggcgcccggg acagataggt
      121 cagggcgcag aagccgccga aactctgccc caggacgctc caccggccgg ccgcggcctc
      181 tccggtgccg gcgctgccgg tgctgccggt gccctcgccc agcaggtgcc ggcggaagag
      241 ttcggcgtcc cgcacgatgg cgtcggcgcg gaagtgcgcc aggtacgcgg cctgttcgcg
      301 cggcccgccg cgcagcgcga gcgtctgccg gttggccggt gtcgagcgcc ccgtaccccg
      361 ctggtccagg agcagtacgc ggtagtggtc aagggcgggt acgagccagg agtcccggcc
      421 cagggggcgc ggcgaggcgc agccggggcc gccctggagg tagaccagcc acggcagttc
      481 ctcgcgctcc cgcccggcgg ccaccacctc ccggccgtac acggacagcc gctcgccgtc
      541 cgggaccgag tggtccagcg gcacgtccag gaagtgatcg cgcagtacga cgccgggcag
      601 ccggtcgacg ctcatggaac tcctcagctc ttgtccgtat gccggcactt gcctgcattc
      661 cggtactcgt ccgtactcat ccgcactcgg cggttctcgt ccgtacttgt tcgtgtcctt
      721 cgcgttcccc tggggtgtcc ggggcccggc ggcccggtca gggtagcgcc ggggcggttc
      781 cgcaattcgg ccgggccgat gcctatcgcg cgcaaggacg gaaggctagg aatggcacca
      841 cacattcatg aggtggtgcc ggtggacggg acacccaggt acccgccgcg tgacgaggag
      901 tcgcaccggc ggctggtgta cggcgaggag tccgcgctcg gcgaggcgta cgacgccttc
      961 ggcgcgctgg tgcgggcggt cgcccggcgc gtcaccggca gcgcgcacag cgccgacgag
     1021 gtggtgcagg aggtcttcct gcggttgtgg acctgcccgt acgccttcgc gcccgggcac
     1081 ggctcgctgc gtgcctggct gagcatgctc gcgcaccacc gcgccgtgct ccggatgcgg
     1141 gacgcggccg gggagccggg cgagcccgga gaatccgagg aagcggggga agcgggggag
     1201 gaggccggtg aggccgggcg gcgggcgcgc gacgccgaag cggcgctgat gctgcactcg
     1261 gcactggccg aactcccgct gccgcagcgg gaggcgatgc atctggcctg cttcgccggg
     1321 cggacctgtc gtcaggtcgc ggtggaactg ggcatccccg aggagatcgc cgcctcccgg
     1381 ctgcgtacgg cgctcggtgg cctcgcggac cgtctcaccg cactgccggg ccatgtcccc
     1441 gccgatcccc gaacctctca aagcccccag gagtcacaag agacccaaga gctccaaggg
     1501 gcccaagagc tcccacaagc atcacccccc ggccccggcg acccggaaag cagcgccgaa
     1561 gacgaccacc aggccgtcag cgagctgctc gcggcctggg tgctgggcgc gggcatgccg
     1621 ggcgacgacg cccgcgtacg ccgccacctg cgaagctgcg cgccgtgcgc cgcggaagcc
     1681 cgtcggctgg gcgaagccgt acgactgctc gacgaaccgg cggagagcgg cgcgacggag
     1741 gcaaagggtg cgaggcaggt aaagggtgta aagggtgcag ggagccggaa aggcggcggc
     1801 aagggggccg tacggaccgt acgcaagggc aacaccgggg tcgcccgcgg ccggctgatg
     1861 gccgccgccc gcaccgcccg gcccggtcac ggcccgctgg ccgcgcacgc cgccccgtac
     1921 gccggggcag tggcgagcct ggacgcgctg ctgcacgaac tggacaccgc tccggccctg
     1981 tggggcaccc ccgtcgtcca cgactgggac gtacagggca ccctcgccca tctcgtcgcg
     2041 gccgacgagg cgctggccga acggctgggg ctggcgccgg tcacgatgac cggcgggagc
     2101 cagggcccgg cccgggtggc gatgccgggc ggcccctggg agctgcgctg gtcggcgcgt
     2161 acggaagagg tggtggcgta cgagcacacc cggccgacgg ccgagacccg cgaggcatgg
     2221 cggacccagg cccacggcct gctggcgctg ccggaggccg gtgacgaaca actggccgcc
     2281 tctcccacgg tgctgatggg actacggctg ccggtcgccg accactatgt gctgcgggcg
     2341 ctggagacct gggtgcacac ccgcgacatc ggacgggcgc tgggccggcc cgtaccgccg
     2401 ccgctgccga cgcatctcca gcggttcgtg ggactgtcca tccgcatcct ggaccgcgcg
     2461 ctggggccga ccgcgcggcc ggtgctgctc tcggtggagg gcgaggcggg cggcgactgg
     2521 gtgctgggct cggacgcaga gccgatcgcc gccgaactgg tcctggaggc ggtggacttc
     2581 tgcctgctgg tcggcggccg gcggattccg gcggagatcg cacgcggcca cgcgggcgac
     2641 ccggtcgcct cccagcgggt actggagacg gccgcatcgc tcgcatggct ctgaccccgg
     2701 taccgacccc ggcactgtcg ggaatcctga aatcccgctg tccgagcgcc tgctccccgt
     2761 gcccgaaccg attgaatcat tatgatcgat tgatggtcgg gcgttcggac cggctgtcct
     2821 cccggcgagt gccgcattag tctgcccgta ttcatgtgcc gcgggggccg ccatgttctt
     2881 gaagacctac gggtgggcaa tggcatgctt cgccctggta ggtgccgcgg tggcgctcct
     2941 gggcggctgg gctcaccttg tgccgctggt tccgatcatg ctgctcggct tcgccctggg
     3001 agtgaacgag gccattgcct cggtcggccc cctcaagcgg atcccggggt tctggcagcg
     3061 cttttttctg ctgccgggag tcatcctcgc cgcggtgatc ggccgcctgg gcgtcccctt
     3121 tttcgtcgtc acgctcagct ccggcgaacc tcccggccag gtgctgagtc tggctctgca
     3181 cgaaccgcat cggtacgagg agatgctcgc cgcggcggaa cccgccgtct acgccttcag
     3241 cggcaccttc ttgctgctgg ccttcttcga attcatcttc atcgaccggg aggtgaagtg
     3301 gctcgcctgg ctggagaggc ccttggggcg agccggaggc atgccgaacc ttgggctcat
     3361 gctcgccatg accgtcctgc tgtgcaccgc ctggttcatc ggcgagcgcc cggcagccat
     3421 cctcaccgcc ggcctgctgg ccctcctgtc ctacgtggtg atccacagca tcgccgcgtt
     3481 cctgcgtgag cgtgcacgcc ctggcagccc aagtaacggg gaaagccgtg ccgtcgacgc
     3541 cggtacggcc ggtgtggcga tcacgctctc cagcatcgag gtggcggaca tcgccgtggg
     3601 ggcggaggcg agtgcggcag cattcatctt cgacaagaac atcttcgtga tagccgccgg
     3661 gctggggatg gccgccttcg tcgcacgctc ctccgtcctg ctgtggacgc gccggcagac
     3721 cttgcgcacc tgggcctacg cacgccatgg cgcggcgtac ggcacgggag cgatcgccgc
     3781 actgctcctg ctcaagacgg gctatgccgt gcccacttac gtcaacgtac tggtggtcgc
     3841 cgtcatgctc ggcgccgcgc tgctctcctc ccaccgcttg gagaaaacaa cagtggagca
     3901 ggccgcatca gaggccgagg gccagcgcac acgatgagcc ggccgaccgc cggccgtacc
     3961 gcggcggcgt tcgtacggaa cggaacgccg ccgcggtacg gccgcacgga gtcaccgcac
     4021 cggcgtgaag tcccgtgcgc cgatgtactc cgggcgccgc accggtgccg cgaacggttc
     4081 caccgccgtg ttctccacgc tgttgaacac gatgaagacg ttgctgcgcg ggtagggggt
     4141 gatgttgtcc ccggagccgt gcatggcgtt gcagtcgaac cacgtcgccg agccgggctt
     4201 cccggtgaac agccggatgc cgtgccggtc cgccatttct gtcagcgccc ggtccgaggg
     4261 ggtgccggcg tcctgcatct ggagggactt cttgtagttg tcccgcggcg tctcgcccgc
     4321 gcagcccagg aagtgccggt gcgaaccagg catgatcatc aggctgccgt tggtgtcgtg
     4381 gttctcggtg agggcgatcg agatcgagac ggtacgcatc cggggcagac cgtcctcggc
     4441 gtgccaggtc tcgaagtcgg agtgccagta gaagcccgag gcaccgaagc ccggcttgac
     4501 gttgatccgg gactggtgga cgtagacgtc cgagccgagg atctgacggg cacgccccac
     4561 cacgcgcggg tcggcgacca ggcccgcgaa cacctcactg atcttgtgca cctcgaagac
     4621 ggaccggatg tcctgggact tcggctcgac gatcgagcgg tcgtccgccc gcatccgcga
     4681 gtcgttgacc aggcggtcca gttcggcgcg gtacaccgcg acctcgtccg cggtgatcag
     4741 ttcgtcgatg gcgaagaagc cgtcgcgggc gaagtcctcc agctcggagg gccggaacgg
     4801 cccggccgtc ccgggctgcg accacaccac cgggtcctga cgcggggtga tcacctcggt
     4861 ggccccacgg gtcgggtaca ggtcggcggt gcgctcgggt gcggtggtca tggtgttgcc
     4921 ttcctctcct ctcggacgtg cttgcgcagc gtgcggcggc gcgtgtgcgc tctcgtacgg
     4981 ctcctgcggg ctcgtacgga tcaggccgcg tcgggttcgg tgagcagcgg gtacacgccg
     5041 ttctcgtcgt ggtcctcgcg gccggtgacc ggcgggttga agacgcacac acagcggaag
     5101 tcggtcttcg gccgcatggt gtgcctctcg tggccgtcca ggaggtacat cgtgccgggc
     5161 gtgatccagt gcttctcgcc ggtctcgtcg ttggtgagtt cggcctcgcc ctccacgcac
     5221 agcacggcct cgatgtggtt ggcgtaccac atggacgtct cggtacccgc gtacagagtc
     5281 gtctcgtgga gggagaagcc gacgcgctcc ttggcgagca cgatgcgctt gctctcccag
     5341 gtgccggact tggccttgac gtggcggtcg gtgccttcga tgtccttgaa ggagcggacg
     5401 atcacggtga ttcggtgcct ttctgtgtct tcttctacga gttctacgag ttctaaaagg
     5461 ttcttctgcc ggcgtacgga cccggtgcgg gtgcgctcag gcgcagtccc gtaccgcgcg
     5521 ggcgagggtg cgaaggccct cgtccagctc gtcgggagtg gtggtcaggg ccggcagcag
     5581 cttgacgacc tcgccctgcg gtccggaggt ctcgatcagc aggccgagtt cgaaggcccg
     5641 ccgggcgacc ttgccggcca gttccttgtc ggggaactcc aggccccaga ccaggccacg
     5701 gccgcggtac tcgccgccca gttccgggtg ggcggcgacg atcgcgcgca ggtgcgactc
     5761 gacgatctcg ccgcggccca gcgtctgctt ctccatctca ccgtcggccc agtaggtgtc
     5821 cagggccgcg gcggcggtga cgaaggcggg gttgttgccg cggaaggtgc cgttgtgctc
     5881 gcccggctcc cacacgtcca gttccggctt gaagagggtg agcgcgagcg ggagcccgta
     5941 gccgctgatg gacttggaga cggtgacgat gtccggcacg atgcccgcct cctcgaagga
     6001 gaagaaggca ccggtgcgtc cgcagcccat ctggatgtcg tccacgatca gcagcatgtc
     6061 gcggcgctcg cacaggtcgg ccagggcccg cagccactcg gggcgggcga cgttgatgcc
     6121 gccctcgccc tggaccgtct cgacgatgac ggcggcgggc cggttcaggc cggagccctg
     6181 gtcctccagc agccgctcga accacaggaa gtccgggtag gtgccgtcga ggtagttgtc
     6241 gaagggcatc ggggtgccgt ggaccagcgg gatgccggcg ccggcccgct tgaaggcgtt
     6301 gccggtcacg gccagcgagc ccagcgacat gccgtggaag gcgttggtga aggacacgat
     6361 ggactcgcgg cccttgacct tgcgggcgag tttgagcgcc gcctcgacgg cgttggtgcc
     6421 cgtcgggccg gggaacatga ccttgtacgg gaggtcgcgc gggcgcagca cgatgttctg
     6481 gaaggactcc aggaaggcgc gcttggccgt ggtggacatg tccaggccgt gggtgacgcc
     6541 gtcgcgttct atgtagtcga tgagcgcccg tttgagtacg gggttgttgt ggccgtagtt
     6601 cagtgagccg gccccggcga agaagtccag gtaggtgtgg ccgtcctcgt cgtacatccg
     6661 gctgccctgc gcgcggtcga agacggtggg ccagccgcgg cagtagctgc gcacctccga
     6721 ctccacggtt tcgaagacgc tcaggtcggg ctgggtgatg gtcacagcat gctcctggga
     6781 gatgggtggc gtgaggaaga agaagtcttt gggggaggtg gggcgtcggg cggccgggcc
     6841 gcgagcggga gcgggggtgg ggacgcgagc gggagtggaa gcacgggcgg gagcgggggc
     6901 gcggcgcggc gcccgccggg ggtcagggcc ggtgcgccgc gagggtgtcc agggggccga
     6961 tgaggtgcag tacctccggc tcgtggccct gctcggggaa gaggccggca tcgaagagga
     7021 cctcgcgccg gtggccgccc gcatggcgct cggcgaagga ggtgaacacc ctctcggagg
     7081 ctgtgttgcc gggggtgacc gtggtttcca ggtaccggat gccgtactcc cgcgcggccc
     7141 gctcggccag tccgtccacc agcgcggcgg ccagcccccg gccgcggtgg gcgtggtcga
     7201 cggcgagctg ccagaccacg agggttccgg ggcgctcggg gcgcagatag ccggtgacga
     7261 agccggccgg ctcgccccgc gcgtcgcggg cgacggcgga ggtggcggcg aagtcacggc
     7321 accacaggag gtagctgtag gacgagttca ggtccagcgt cttggagtcg cgggcgatac
     7381 gccagatcgc ggctccgtcc tccacccgtg gggtgtcgag cttgaatccc tccggcattt
     7441 ccctgatggc gctgatgtcg ctgaagtcac tacgagatcg tgcacggtct gctggtgcgg
     7501 cggtcatgcg aattcaattt acccagggaa aaacaaaaat gcatcgagga tcggggttac
     7561 gcggcccggg gtggatgtgt tatcgcacgg gggcgctcgg gcgcgcgaga gtcctgaaaa
     7621 atgtcactgc ttgtccgaaa aataccgggc aaaacgggcg acatgtgggg tcgatcacag
     7681 ccctgtgacg ccgttacagg gcgcccggaa cgcgccgatt gcggctagcg aaattttagc
     7741 gtttgggctg cgcgaaaccg ggcaggagag cacaggaagc tgtgcttcca aagaataggg
     7801 aattcgccgg taaaagatgc cgagaggtgc tgagaggtgt cgagaggcgc cttgaagtac
     7861 ctccaggtgt accgcgggga aaggagaatg gtccgtgccg ttacggcccg cgttccggcc
     7921 ggctttccgg cccgcgtcac gaggtacgcc accgcgtcgc gtggcccgga gcggtcgcgg
     7981 atacccgcgg acccccgtcc tgccgctgcg catacagtgc gtccatgagt gagagtgtgg
     8041 tgctgcacat caaggggagg gtcctggccg ggccgcagga cgtgcgcgac gaactctggg
     8101 tcgtcgacgg gcgggtgacc tatgaacggc cgggcggggc acgggagatc acgaccgtac
     8161 ggggctgggc gctgccgggc ctggtggacg cccactgcca tgtcggcctg gacgcgcacg
     8221 gcccggtgga cgaggccacc agcgagaagc aggcgctgac ggaccgggac gccggaacgc
     8281 tgctgatccg cgacgccggc tcaccctccg acacccgctg gatcgacgac cgcgaggacc
     8341 tgccgcggat catccgcgcc ggccggcaca tcgcccgtac ccgccgctac atccgcaact
     8401 acgcccacga gatcgagccg gaggatttgg tcgcgtacgt ggcgcgggag gcccgccgcg
     8461 gcgacggctg ggtcaagctc gtcggtgact ggatcgaccg cgagagcggc gatctggcgg
     8521 cctgctggcc gcgcggcgag gtcgaggcgg ccatcgccga ggcacaccgg ctcggcgccc
     8581 gggtgacggc ccactgcttc gccgaggagt cgctggcccc gctcgtcgag gcgggcatcg
     8641 actgtatcga gcacgccacc ggcctgaccg aggagaccat cccgctcttc gccgagcgcg
     8701 gcgtcgcgat cgtacccacg ctcatcaaca tcgcgacctt cccgacgctc gcggcggccg
     8761 gcgagggcaa gttcccgcgc tgggcggacc acatgcgccg gctgcacgaa cgccgctacg
     8821 acaccgtacg ggccgcgtac gacgccggag tgcccgtctt cgccggcacg gacgcgggcg
     8881 gctcgctcgc ccacggcctg gtcgcccagg aggtggccga gctgatgaag gcgggcattc
     8941 ccgcggtgga cgccctctcg gcgacgacct ggagcgcccg tacgtggctg ggccgccccg
     9001 gcctcaccga gggcgccccc gcggacctcg tcgtctacga cagcgacccg cgcgaggacg
     9061 tccgcgtcct ggcggcacca cgccgggtgg tgctgcgggg acgggtggtc ggctagggaa
     9121 ccttccgtac agggacgggc cttacaggga cgggccttcc cggacggggt ggccggtcgg
     9181 cggcacagga ccgggtcggt gtccggcgct gaagcgggcg gtgaccgcag acgcgtccga
     9241 gccggagccg tgcacgccga caccgacgcc gacgccgacc tccaaggcca ccgcccggac
     9301 cgcccggacc gccggcacgc cgggccggcc cggtcacgct ccccgcaggc acaaccgagc
     9361 gccggcccca ggccgttgtg cgcaccacgc ccaccaacag ggcatccccg gcgccgcggc
     9421 gtccggacgc gcgccgcacc gcccgccacc accgtcagcc tgatcgccgc aacggtcggc
     9481 gcgaagcccc ggggcccagc gcggagcatg acggaggagg cgggtagggg agggagcggg
     9541 taacccgtgc gcctacgggg taaatcgaac gcccctgtcc cccggtcggg ggttacccgg
     9601 aggggcgggt gcggaacccc cgcgctggac aggggcgggg tgagggggag cgggcggagg
     9661 cagagccctt tctttcccgt tgctgcatga gtgcggcagg gctggctggt ggatgaaggg
     9721 acgccacggt cggggacagc ggactccgtc cgaaatgacc gtgcatactc cgtccaccct
     9781 gcgggctggc gctcgtattt cctcaccggc gagagcagga agcacatctc tccgcgcgcg
     9841 cttcgtgtac gggctcttcg gccggtcagg cgtggcggca ccgggcgggc gttgtcgccc
     9901 tcaacggctc agtgcggccc ggctgttcgt acgcttcgga caggaggaac tcagagctgc
     9961 accagggcaa agacgcactt ggccgtggcg catgccactg accgcatctg ctcgtcgggg
    10021 gccgtgggtg tcggtcaggc ggtcatgagc cggtggcgtg ttgcatgcgt gcggcggtcc
    10081 ggtggaggta ggcggtcacc tcggggtcgg catcgagggt gtggtcggcg tcgagggcgg
    10141 cgagggcctg ggcgatgccg gggaggtggt tgctggagaa gtcgacggtg caggtggcct
    10201 cgtcacgggg gcggatgcgg gcggccaggg cccaggtgcg gcggcggacg gtgtgggcgg
    10261 gggcgtggac ggtggcgatg gcgcggtagc gggtcggcgc ggtggcgatc ttgttggcga
    10321 cataggcggc cggatcgttg ccggggacgg cgcgggggtg aagtggtggc gggtgggggc
    10381 cgggttggtg atgcggtcga gacgg
//
