LOCUS       NZ_CP074380            27899 bp    DNA     linear   CON 27-MAY-2024
DEFINITION  Streptomyces sp. MST-110588 chromosome, complete genome.
ACCESSION   NZ_CP074380
VERSION     NZ_CP074380.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. MST-110588
  ORGANISM  Streptomyces sp. MST-110588
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7915503)
  AUTHORS   Booth,T.J., Bozhueyuek,K.A.J., Liston,J.D., Lacey,E. and
            Wilkinson,B.
  TITLE     Bifurcation drives the evolution of assembly-line biosynthesis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7915503)
  AUTHORS   Booth,T.J., Bozhueyuek,K.A.J., Liston,J.D., Lacey,E. and
            Wilkinson,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAY-2021) Molecular Microbiology, John Innes Centre,
            John Innes Centre, Norwich, Not US or Canada NR4, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 28-FEB-2016
            Assembly Method                   :: HGAP v. 3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 25.0x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022695595.1-RS_2024_05_27
            Annotation Date                   :: 05/27/2024 05:38:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,918
            CDSs (total)                      :: 6,828
            Genes (coding)                    :: 6,327
            CDSs (with protein)               :: 6,327
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 501
            CDSs (without protein)            :: 501
            Pseudo Genes (ambiguous residues) :: 0 of 501
            Pseudo Genes (frameshifted)       :: 350 of 501
            Pseudo Genes (incomplete)         :: 185 of 501
            Pseudo Genes (internal stop)      :: 14 of 501
            Pseudo Genes (multiple problems)  :: 45 of 501
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 12:56:23
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 5392793
            Orig. end                         :: 5420692
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP074380.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Barrie Wilkinson, John Innes
            Centre, Norwich.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..27899
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="terpene"
                     /region_number="22"
                     /rules="(PT_phytoene_like or phytoene_synt or Lycopene_cycl
                     or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA
                     or TS_Pyr4)"
                     /tool="antismash"
     cand_cluster    1..27899
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /kind="single"
                     /product="terpene"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..27899
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="False"
                     /core_location="[10000:17899](-)"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..17899)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
     gene            241..1611
                     /locus_tag="KGS77_RS23570"
                     /old_locus_tag="KGS77_23405"
     CDS             241..1611
                     /GO_function="GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1087: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018541958.1"
                     /locus_tag="KGS77_RS23570"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_23405"
                     /product="FAD-dependent monooxygenase"
                     /protein_id="WP_242584993.1"
                     /transl_table=11
                     /translation="MPSTTTRATRLAPSPSPPVRPTQPGHPSTTPAPRSGRPAGPSVLI
                     SGASVAGPALAYWLRRYGYRPTVVEVAPALRDGGFAVDFRGAAHLTVLERMGILDRIRQ
                     HTTGGGSPMTFIDTQGRELASLPAEFAAGELEILRSTLSRLLYEHSAQAPTPHHRPGYT
                     TEATPPTEYVFGDSITSMTETATGVEVTFERSAPRIFDLVIGADGLHSTVRRLAFGPEE
                     DFVGHLGYYVAGWDLTAGSDISDRSVGYGEPGRMVSVGRMPRRTTEAGYHGEVFVVFAS
                     KKLAYDRRDPQAQKQLIAQAYAGAGWRTAELIDTLWEAQNLYFDSISRVDIPTWSKGRI
                     TLLGDAAYGATLGGMGTGAAIVGAYVLAGELAAADGDHRRAFSRYEQLMRPYVTQCQQG
                     GQGVGEFLAPRTAEGIETRNHMLNDPDQLAAMFQQGHDISADITLPLYPYPSLHPTQ"
     gene            complement(1620..2827)
                     /locus_tag="KGS77_RS23575"
                     /old_locus_tag="KGS77_23410"
                     /pseudo=""
     CDS             complement(1620..2827)
                     /GO_function="GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015608292.1"
                     /locus_tag="KGS77_RS23575"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_23410"
                     /product="acyltransferase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MLPSGVISSGGNVAPGQRDRAIDGLRALALLAVPTGHWLIGGLAR
                     DADGALHNESPLSSFAPLAPITWVLQMLGLFFLVGGFASVQSYRRAQGRGSRRGRGFGG
                     GWPG"
     gene            complement(3153..3965)
                     /locus_tag="KGS77_RS23580"
                     /old_locus_tag="KGS77_23415"
     CDS             complement(3153..3965)
                     /EC_number="1.1.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_KR (6-161). E-value: 1.1e-17. Score:
                     56.5. Matches aSDomain:
                     nrpspksdomains_KGS77_RS23580_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short_C2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016576057.1"
                     /locus_tag="KGS77_RS23580"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_23415"
                     /product="SDR family oxidoreductase"
                     /protein_id="WP_242584994.1"
                     /sec_met_domain="adh_short (E-value: 2e-35, bitscore:
                     121.0, seeds: 230, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short_C2 (E-value: 2.2e-36, bitscore:
                     124.7, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSEQQAVIVTGGGTGIGAATARLLREAGHQVVVSGRRPEPLHHIA
                     EETGALAHPSDIGDPDAVRDLVDTAVATYGRLDGLVLNAGIGRSGTVGELSDEDWEAVM
                     RVNLTGPFLLLRAALPHLLKSRGSVVAVASVSALRNGVGNAAYATSKAALLQLCRSLAV
                     DYGPQGLRANTVCPSWVRTDMADRRMARFAEEAGLGDGGTEAAYTEATRLLPARRPGDP
                     REVAEAITWLLSPAASFVNGAVLTVDGGATALDPGTAAFTYRLEPRTP"
     aSDomain        complement(3483..3947)
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_KGS77_RS23580_PKS_KR.1"
                     /evalue="1.10E-17"
                     /label="KGS77_RS23580_PKS_KR.1"
                     /locus_tag="KGS77_RS23580"
                     /protein_end="161"
                     /protein_start="6"
                     /score="56.5"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="VIVTGGGTGIGAATARLLREAGHQVVVSGRRPEPLHHIAEETGAL
                     AHPSDIGDPDAVRDLVDTAVATYGRLDGLVLNAGIGRSGTVGELSDEDWEAVMRVNLTG
                     PFLLLRAALPHLLKSRGSVVAVASVSALRNGVGNAAYATSKAALLQLCRSL"
     CDS_motif       complement(3855..3947)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_KGS77_RS23580_0001"
                     /evalue="2.10E-03"
                     /label="PKSI-KR_m1"
                     /locus_tag="KGS77_RS23580"
                     /protein_end="37"
                     /protein_start="6"
                     /score="10.7"
                     /tool="antismash"
                     /translation="VIVTGGGTGIGAATARLLREAGHQVVVSGRR"
     rRNA            4755..6283
                     /db_xref="RFAM:RF00177"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /locus_tag="KGS77_RS23585"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /old_locus_tag="KGS77_23420"
                     /product="16S ribosomal RNA"
     gene            4755..6283
                     /locus_tag="KGS77_RS23585"
                     /old_locus_tag="KGS77_23420"
     misc_feature    4798..4814
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 21.19"
                     /tool="antismash"
     misc_feature    5175..5191
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 17.97"
                     /tool="antismash"
     misc_feature    6009..6022
                     /note="TFBS match to CatR, H2O2-responsive repressor,
                     confidence: medium, score: 19.27"
                     /tool="antismash"
     rRNA            6588..9709
                     /db_xref="RFAM:RF02541"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /locus_tag="KGS77_RS23590"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /old_locus_tag="KGS77_23425"
                     /product="23S ribosomal RNA"
     gene            6588..9709
                     /locus_tag="KGS77_RS23590"
                     /old_locus_tag="KGS77_23425"
     misc_feature    complement(7015..7029)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 16.81"
                     /tool="antismash"
     misc_feature    complement(7340..7358)
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 21.27"
                     /tool="antismash"
     misc_feature    8572..8585
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 16.92"
                     /tool="antismash"
     misc_feature    8573..8586
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 14.67"
                     /tool="antismash"
     misc_feature    complement(8574..8587)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 15.1"
                     /tool="antismash"
     misc_feature    complement(8575..8588)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 15.03"
                     /tool="antismash"
     misc_feature    complement(8581..8590)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 14.85"
                     /tool="antismash"
     misc_feature    complement(8655..8670)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.11"
                     /tool="antismash"
     misc_feature    8724..8737
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 15.96"
                     /tool="antismash"
     misc_feature    8806..8826
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 20.46"
                     /tool="antismash"
     misc_feature    complement(8819..8829)
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 18.94"
                     /tool="antismash"
     misc_feature    8994..9012
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: weak, score: 20.29"
                     /tool="antismash"
     rRNA            9786..9902
                     /db_xref="RFAM:RF00001"
                     /gene="rrf"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /locus_tag="KGS77_RS23595"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /old_locus_tag="KGS77_23430"
                     /product="5S ribosomal RNA"
     gene            9786..9902
                     /gene="rrf"
                     /locus_tag="KGS77_RS23595"
                     /old_locus_tag="KGS77_23430"
     misc_feature    9898..9911
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 18.27"
                     /tool="antismash"
     misc_feature    complement(9903..9917)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 16.3"
                     /tool="antismash"
     misc_feature    9904..9917
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 14.84"
                     /tool="antismash"
     gene            complement(10001..11584)
                     /locus_tag="KGS77_RS23600"
                     /old_locus_tag="KGS77_23435"
     CDS             complement(10001..11584)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: T2TS"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003957289.1"
                     /locus_tag="KGS77_RS23600"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_23435"
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="WP_242584995.1"
                     /sec_met_domain="T2TS (E-value: 3.2e-80, bitscore: 268.8,
                     seeds: 58, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKNHTPFDLHAAKLLNEATADDLGQSTPSVYETARLVSDAPWLEG
                     HSRRVRFLCERQNPDGTWGTAEHGLLQTLSAAEALLTVLARGDDVHVPRPQLVNTTALA
                     VTRLSHSLGNHQCPPTLPDTVAVELIVPALIDRIHQHLGRCARSRPPFLLPWASSILLA
                     RPKGCLPSVLAELRAAVYQGEPLPEKLWHTWEILAPATEHPDRHIRPYEGAVSCSPAAT
                     AAWLGRPHDSERRQAGLRYLHRIQSRYGGPVPVAAPITTFERAWIAGLFAHHGVPHTPP
                     PALLAGLRDALGEAGAPGSPGLPPDADDTAAVLYALLHHGDHIRPDSLMPYFTDDHFCC
                     YPEERSPSSSTNAHALEALVLYVARHPADRGRYRSVIRRTIDWLLAQQHPDGSWSDKWH
                     ASPYYATACCVRALAAFAGPVAHPALARASAWALATRLPGGRWGHGANGTVEETAYAVW
                     ILLGRTPQTGAARQALTSARRILQAECVTDNIPALWHDKDVYAPLRILRATRLAALHTL
                     YHLPRRRPAS"
     gene            complement(11767..12774)
                     /locus_tag="KGS77_RS23605"
                     /old_locus_tag="KGS77_23440"
     CDS             complement(11767..12774)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: T1TS"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1052: Terpene synthase/cyclase metal-binding domain
                     protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF040195.3"
                     /locus_tag="KGS77_RS23605"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_23440"
                     /product="hypothetical protein"
                     /protein_id="WP_242584996.1"
                     /sec_met_domain="T1TS (E-value: 2.4e-39, bitscore: 134.6,
                     seeds: 237, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTEPVRAWNKVNDGTDRTPMYESLQRHFPQLAGVWAAQPPEFTR
                     IGHAVERHIRAWAEEHHLMENSVVRDREYRSRLALGIHLGWWKVTDPVMAEIIGEGMWW
                     LTVFDDVHCEERARADMPGFARRITTMMRMLEPGARPTEPGFASALAAFVARLRATASG
                     RMTERVVMAYHAAFAAMLCEALLEDPADVTLEDFTVLRRHVVYGHISVLFAELAMECDL
                     PPEVRDSPTVHDLRMAVANDIGWVNDIYSAFREHDVRGGLPFSLPGVLAAHHAYGLDEA
                     VDRSVAILDAEIARVRRLSDELAASPDPQLRAFAQGMAVLVSSNGWYEANHFYR"
     misc_feature    13036..13051
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 21.32"
                     /tool="antismash"
     gene            complement(13277..13447)
                     /locus_tag="KGS77_RS23610"
                     /old_locus_tag="KGS77_23445"
     CDS             complement(13277..13447)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="KGS77_RS23610"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="KGS77_23445"
                     /product="hypothetical protein"
                     /protein_id="WP_242584997.1"
                     /transl_table=11
                     /translation="MLSQPRGLLFWLVLITFLFLVAIAPVQTAHTLLDMAHGITKFFTG
                     LKKFVETLSAS"
     gene            complement(13493..15028)
                     /locus_tag="KGS77_RS23615"
                     /old_locus_tag="KGS77_23450"
     CDS             complement(13493..15028)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010039650.1"
                     /locus_tag="KGS77_RS23615"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_23450"
                     /product="AarF/UbiB family protein"
                     /protein_id="WP_242584998.1"
                     /transl_table=11
                     /translation="MATGRARLVLTTFGTLLVREGAHTLARHTRHRDADGESAARDRAR
                     ALREALESLGPFYIKVGQILSTRPDLVSPTLIEELQTLHDQVSPSPFADFEPVMAGDLG
                     PLWAAGFRDIDTDRPLGTASLAQVYAATLADGTPVALKIQRPGVRPLVEADMALLTKAA
                     RFLARQSPRFNAVIDVSAMLGVVFDAMRPELDFTLEARNMHQARTHTADFKHLTVPTPI
                     SATPRVLIQSLAPGCSIADARPHDFSLEERAGIGRDLLAFMYRSYFLTRTFHADPHPGN
                     ILVHPGQPAHLIDWGMVGRIDRPLSTSILLVLLSLAQNDGAATAKAWIEMGHATPWAEV
                     TDFIEDMAALVPQIATATLEELNFGVTLTAILEHSTQRGIKTSPMISVLGKSFANLEGS
                     IRNLCPELSLIDIFEEEFRHIAFRLATEALSEKQAARTALDLILGSATAPQQTRSIIRD
                     LSNRELTVQVNQLPMNRALQNLPLSNHKVLRDSLAVLLALTWWRRHRHHQPPT"
     gene            complement(15070..15438)
                     /locus_tag="KGS77_RS23620"
                     /old_locus_tag="KGS77_23455"
     CDS             complement(15070..15438)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF019494.4"
                     /locus_tag="KGS77_RS23620"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_23455"
                     /product="polyhydroxyalkanoate synthesis regulator
                     DNA-binding domain-containing protein"
                     /protein_id="WP_242584999.1"
                     /transl_table=11
                     /translation="MTVPGGPGRLPPAGAGSPRRHRHRPGPPPDRVLNRHGGGRLIDVR
                     LHEPVTLDELIQDLRASRQFVVRDHRTGEDCTYQVLAEVIAWALGAWVPTAAAHSPLNP
                     ATLLGDPPRRGATPTGGR"
     gene            complement(15435..15761)
                     /locus_tag="KGS77_RS23625"
                     /old_locus_tag="KGS77_23460"
     CDS             complement(15435..15761)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="KGS77_RS23625"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="KGS77_23460"
                     /product="hypothetical protein"
                     /protein_id="WP_242585000.1"
                     /transl_table=11
                     /translation="MARRTGITKTVSNIVDDIKDMLDDALDSVCDLEHDTRDSLSRSLR
                     PERERDTERDTERRRTGPDEARSPQLEMAALWSKLGQIEELLRQQQEGTVAAQPAGRAK
                     QPSS"
     gene            complement(15846..16829)
                     /locus_tag="KGS77_RS23630"
                     /old_locus_tag="KGS77_23465"
     CDS             complement(15846..16829)
                     /EC_number="1.17.7.4"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_function="GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA]"
                     /GO_process="GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948231.1"
                     /locus_tag="KGS77_RS23630"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_23465"
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="WP_242585001.1"
                     /transl_table=11
                     /translation="MSTPTARRRVLLAAPRGYCAGVARAVTVVEKALELYGAPVYVRHE
                     IVHNKYVVKSLEHRGAVFVDEATEVPEGATVILSAHGVAPGVHEEAAARRLTTIDATCP
                     LVTKVHKEALRFAREDYDILLIGHAGHEEVIGTSGQAPDHITVVDGPDDAATVEVRDPE
                     KVAWLSQTTLSVDETTATVAALRARFPHLLSPPGDDICYATQNRQAAVKRLGADADLVL
                     VVGSRNSSNSVRLVEVALDAGAGAARLVDTAEDIDEGWLTGVRTVGVTSGASVPDLLVD
                     GVLDRLATYGFTRIDVVRAAEEHQLFALPRNLVPRTTGPPPPDAPA"
     gene            complement(16826..17899)
                     /locus_tag="KGS77_RS23635"
                     /old_locus_tag="KGS77_23470"
     CDS             complement(16826..17899)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: PT_FPPS_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1182: Polyprenyl synthetase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014058774.1"
                     /locus_tag="KGS77_RS23635"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_23470"
                     /product="polyprenyl synthetase family protein"
                     /protein_id="WP_242585002.1"
                     /sec_met_domain="PT_FPPS_like (E-value: 6.3e-51, bitscore:
                     171.8, seeds: 145, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTAALETPLDLPLLRKRIDSCLDHYLTVKAYAARTENLPALPLE
                     VLRDFLFAGGKRIRPLMCVLGWYAARGRGEADPVLRAAASLELFHAFALIHDDIMDRSD
                     TRRGHPTVHRTLAERHQHRTHHIEFGEHAAILLGDLALACSQEMLNGAGLSTAQLTVAL
                     PTLDAMRTAVLYGQYMDLDTAEQLTDDVERALAVVRYKTAKYTVQHPLHLGAALAGSGP
                     AVRAALSAYALPLGEAFQLRDDLLGVFGDPAVTGKSVLDDLREGKRTVLLALALRHADA
                     RQARTLRHLVGDPDLDEEQAATLRTVLAGTGARARVETLIDSHLHTALRALDCAPFPAD
                     ARDALREVAHTLTERAL"
     gene            complement(<18040..19194)
                     /gene="ispG"
                     /gene_synonym="gcpE"
                     /locus_tag="KGS77_RS23640"
                     /old_locus_tag="KGS77_23475"
                     /pseudo=""
     CDS             complement(<18040..19194)
                     /GO_function="GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA]"
                     /GO_process="GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ispG"
                     /gene_synonym="gcpE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009068955.1"
                     /locus_tag="KGS77_RS23640"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_23475"
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MTTTNLPIPEVRARPAGSPPYPPRPPSPAAAPPQTPERRRSRQLH
                     VGDVPVGGGAPVSVQSMTTTPTTDIDATLQQIAELTAAGCQIVRVAVPSQDDADALPAI
                     AAKSRIPVIADIHFQPQYVFAAIDAGCAAVRVNPGNIKKFDDKVKEIARAASDAKVPIR
                     IGVNAGSLDRRLLAKYGKASPQALVESALWECSLFEEHGFTDLKISVKHHDPLTTVAAY
                     RRLAAACDYPLHLGVTEAGPPPQGTIKSATAFAILLAEGIGDTIRVSLTAPPVEEVKAG
                     LQILQSLGLRRRQLEIVSCPSCGRAQVDVYRLTERVAAALDGFPHPLRVAVMGCVVNGP
                     GEAREADLGVASGNGKGQIFRKGAVIKTVPESKIVEALVEEALRL"
     gene            complement(19391..21196)
                     /locus_tag="KGS77_RS23645"
                     /old_locus_tag="KGS77_23480"
     CDS             complement(19391..21196)
                     /EC_number="2.2.1.7"
                     /GO_function="GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA]"
                     /GO_process="GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DXP_synthase_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1110: 1-deoxy-D-xylulose-5-phosphate synthase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006349430.1"
                     /locus_tag="KGS77_RS23645"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_23480"
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="WP_242585003.1"
                     /sec_met_domain="DXP_synthase_N (E-value: 2.7e-86,
                     bitscore: 287.8, seeds: 174, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAAIRWSPQALRALSPRETVAVAERIRTFLIENVCGTGGHLGPNL
                     GAVELTLALHRVFDSPRDALIFDTGHQAYVHKIITGRAHGFDRLRQRGGLSGYPSRSES
                     VHDWVENSHASTSLAYADGLGKAFAARGEGDRWVVPVIGDGALTGGLAWEALNNLGTAG
                     RRRIAVVLNDNGRSYAPTTGALADHLSALRRPLAQADAPVSAYVDGPPEARNLFTCLGF
                     TYLGPVDGHDLDAVEAALRQAREIGGPVLVHTVTVKGKGYVPAETDDADCLHAVGVVDP
                     ATGTARDTAAPTWTDVFAGELAELGAGREDVVAVCAAMLRPTGLGPFADRFPGRVFDVG
                     IAEQQAMCSAAGLSMGGLHPVVALYATFANRAFDQLLMDVALHRLPVTLVLDRAGITGP
                     DGPSHHGMWDHTLLSLVPGLRLAAPRDATTLRTLLGEALADQDGPTALRFPKGAVGADV
                     PAVGRMGQVDLLSAPGRDVLLVGLGAMAAPCLAARERLAARGVEATVAAPRWVLPLPPE
                     LVQLAARHTLVVTVEDCARTGGVGSALAQACTDAAVAAPVHSLGLPRTFLTHGSRAELL
                     AATGLDAGGIATAVHLAWNARHRRR"
     misc_feature    complement(21155..21157)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(21565..21705)
                     /locus_tag="KGS77_RS23650"
                     /old_locus_tag="KGS77_23485"
     CDS             complement(21565..21705)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="KGS77_RS23650"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="KGS77_23485"
                     /product="hypothetical protein"
                     /protein_id="WP_242585004.1"
                     /transl_table=11
                     /translation="MPEDALAVVVRPGPELPEHRTRQGRNSDRPPARTAAVRVCAWGER
                     T"
     misc_feature    complement(22011..22025)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 16.84"
                     /tool="antismash"
     gene            22217..22915
                     /locus_tag="KGS77_RS23655"
                     /old_locus_tag="KGS77_23490"
     CDS             22217..22915
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_KR (3-145). E-value: 2.9e-11. Score:
                     35.5. Matches aSDomain:
                     nrpspksdomains_KGS77_RS23655_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358036.1"
                     /locus_tag="KGS77_RS23655"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_23490"
                     /product="SDR family oxidoreductase"
                     /protein_id="WP_242585005.1"
                     /transl_table=11
                     /translation="MESVALITGGSTGIGAATARALLKQGHRVAVTGRDAGRLAAFAAS
                     AGAGEQLLTFTGDASDASHVASAVRQVVDTWGRLDTVIANAGFSLPGTLEDQDPEAMRA
                     MVLTNVLGPSLLVRETLPHLRESKGRIVIVGSVAGVRHTPGNLYSVTKWAAHALAENTR
                     LLVTKDGVGVTLVAPGIVDTPFWDNRGGRPDTSPAMTAEQIAEAILFAVNQPEGMDVNH
                     ITMRPLGQAN"
     aSDomain        22226..22651
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_KGS77_RS23655_PKS_KR.1"
                     /evalue="2.90E-11"
                     /label="KGS77_RS23655_PKS_KR.1"
                     /locus_tag="KGS77_RS23655"
                     /protein_end="145"
                     /protein_start="3"
                     /score="35.5"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C1"
                     /tool="antismash"
                     /translation="VALITGGSTGIGAATARALLKQGHRVAVTGRDAGRLAAFAASAGA
                     GEQLLTFTGDASDASHVASAVRQVVDTWGRLDTVIANAGFSLPGTLEDQDPEAMRAMVL
                     TNVLGPSLLVRETLPHLRESKGRIVIVGSVAGVRHTPG"
     CDS_motif       22232..22318
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_KGS77_RS23655_0001"
                     /evalue="3.10E-06"
                     /label="PKSI-KR_m1"
                     /locus_tag="KGS77_RS23655"
                     /protein_end="34"
                     /protein_start="5"
                     /score="19.8"
                     /tool="antismash"
                     /translation="LITGGSTGIGAATARALLKQGHRVAVTGR"
     misc_feature    complement(22949..22964)
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 22.23"
                     /tool="antismash"
     misc_feature    22950..22963
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 19.59"
                     /tool="antismash"
     tRNA            complement(23144..23216)
                     /anticodon=(pos:complement(5415974..5415976),aa:Arg,
                     seq:tct)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="KGS77_RS23660"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="KGS77_23495"
                     /product="tRNA-Arg"
     gene            complement(23144..23216)
                     /locus_tag="KGS77_RS23660"
                     /old_locus_tag="KGS77_23495"
     misc_feature    complement(23492..23508)
                     /note="TFBS match to NtrC, Nitrogen regulatory protein C,
                     confidence: medium, score: 22.71"
                     /tool="antismash"
     gene            23519..25246
                     /locus_tag="KGS77_RS23665"
                     /old_locus_tag="KGS77_23500"
     CDS             23519..25246
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019886402.1"
                     /locus_tag="KGS77_RS23665"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_23500"
                     /product="dynamin family protein"
                     /protein_id="WP_242585006.1"
                     /transl_table=11
                     /translation="MATSDVRPRIIDALSALRDRVDAARFPLPLPGAARARRGRCELLA
                     QLDDYVMPRLRSPQAPLLAVVGGSTGAGKSTLVNSLVGRRVSEAGVLRPTTRTPVLVCH
                     PDDHHWFAGQRVLPQLARIRTPGQEDAGAGAAAGAEALRIETDEALPSGLALLDAPDID
                     SLVARNRELAAELICAADVWVLVTTAARYADAVPWHLLRTAKEYDVTLVTVLDRVPHQI
                     ALDVSARYAALLARAGLADVPRFTIPELPESAGGGSGLLPATAVAGLREWLERHARDPI
                     ARTAAAHRTSAGIIASLRRRMPALAGACAAQHAAALRLADRVEEAYEEAAERVRKEIAE
                     GEVLSGDARAHWRDHALGGRPDELLDALTRGLTSLLRCAVEQADERIADAWRRDSVALA
                     ARPDTAAVGVLGEGWVNGPEVRPGMRLGVRPGMRSGMRWRTGRPGGWGFSYGVGGAALR
                     NWPRTRCGRCGRNVGREGHEEHEGLWGYEGWAPGSVRVRSTRRKRRRCWPSRCSAGGGR
                     GRRGSTWRDCWGPMARCGCGTGGNGCSGRTWSTRWTVSGNDGWLRWSGGECRRPIRSG"
     misc_feature    complement(26864..26878)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.92"
                     /tool="antismash"
     misc_feature    27846..27860
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 18.75"
                     /tool="antismash"
ORIGIN
        1 ggcgatggcg gtgcgggtga tccggtccag gctcagagcc ggcttggggc cgcggctggg
       61 gcgttcccgc ccgccccaca gcaactccac gctgcccgcc tggtccccga aaacgctgat
      121 cttcctgtct tcagccactc ccgcatttta aaactgtgtc cgtggtatac agatcagtgt
      181 ccgccggata cagtttctct cgtcaagcca acacccgaca cccgacgcaa ggagcgcaaa
      241 atgccctcta caaccacgcg cgcaacacga ctggccccct cacccagccc acccgtccgt
      301 cccacccaac ccggccaccc ctccaccacc cccgctcccc ggtccggacg tcccgccggt
      361 ccgagcgtcc tcatctccgg ggcgagcgtc gccggtccgg ccctggcgta ctggctcagg
      421 cggtacggct accgccccac cgtggtcgaa gtggcaccgg ccctgcgcga cggcggcttc
      481 gcggtcgact tccgtggggc cgcgcatctc accgtgctgg agcgcatggg gatcctggac
      541 cggatccggc agcacaccac cggcggcggc agccccatga ccttcataga cactcaggga
      601 cgcgagctgg cctcgctccc cgccgagttc gcggccggcg agctggagat cctccgctcc
      661 accctctccc gcctcctgta cgagcacagc gcgcaagccc ccaccccgca ccaccggccc
      721 gggtatacaa ccgaggccac cccacccacc gagtacgtct tcggtgactc cataacctcc
      781 atgaccgaga ccgcgaccgg cgtagaggtc accttcgaac ggagcgcccc gcgcatcttc
      841 gacctggtga tcggcgcgga cggcctgcac tccaccgtcc gccggctggc cttcggcccc
      901 gaggaggatt tcgtcggcca cctcggttac tacgtcgcgg gctgggacct gaccgccggc
      961 tcggacatca gcgaccggtc ggtgggctat ggcgagcccg gccggatggt cagtgtgggc
     1021 cggatgcccc gacgcaccac cgaggcgggt taccacgggg aggtcttcgt cgtcttcgcg
     1081 tccaagaagc tggcgtacga ccgacgcgac ccgcaggcac agaagcagct catagcccag
     1141 gcgtacgccg gagccggctg gagaacggcg gagctgatcg acaccttgtg ggaggcacag
     1201 aacctctact tcgactccat cagccgggtc gacataccca cctggtccaa gggccgcatc
     1261 accctcctcg gcgacgccgc gtacggggcg acgctcggcg ggatgggcac cggcgcggcg
     1321 atcgtgggcg cgtacgtact cgcgggcgag ctggccgccg ccgacggcga ccaccgccgc
     1381 gccttctccc gctacgaaca gctcatgcgc ccctacgtca cacagtgcca gcagggcggc
     1441 caaggcgtcg gcgagttcct ggccccccgc accgccgaag gaatcgagac gcgcaaccac
     1501 atgctgaacg accccgacca actggcagcc atgttccaac agggccacga catctcagcc
     1561 gacatcaccc tgcccctcta tccctacccc tccctgcacc ccacccagta gctgtccctt
     1621 caggcgagcc ccagcgcgaa tccggcgaat cccgtggcca aggctcccgc ggcgatccgc
     1681 tgtgcccggg tcactccggt ccaaggcgcc tcgaaccggc gggtggcccg cgcgatcacc
     1741 acgaggagaa ccgcgaacag aggcagccag ggaagccgcg tcagcatcca cgcggggaag
     1801 tccggcgcgg tggtcagccc gtgcaccgtt cccaggaagg ccgtagggac ggcagcggcg
     1861 agcatcgcgg tctggtgcca gcaaagaatc gtcatggcag agccgttgac cgccgaaacc
     1921 ggagcccaca gagcgggttt ccgcaacagc gccgcgatac ggtcccgcag cagaatggcc
     1981 gccccggact gcgcggcggc cagggcgatg accaagagcg acggtggatg ggaattcgta
     2041 cggtcctgcc cggggacccc gaccatgctc gccgggtaat ggaacaccag caacagcaca
     2101 acaaagaccg ccgtcccgcc cacgaacagc agccaggccc cccgccgccc gagccgcttc
     2161 tcgccccagg aaacccccaa ctgataggcg aacatccacc cgggaaacag actgatcagc
     2221 ccgacccatt caggtaccga cgcggcgaaa ggaccgtaac gcaggaagtc cacgaccgcg
     2281 atggtcgccg ccagcggcgc cgccgcccac ccaccgagcc gccgggaggc cgccacacaa
     2341 tagggagtca gcgcggtcac cacggcatac acgcacacga accacaacgg ctgcacgacc
     2401 agcgtcgccc cggtgcgcaa cgtcaccaaa ggcaccccga ccgcgtacaa cagcggcgcc
     2461 agcaacgccc agaccgccgt cacccccacc gcgggccgaa tcaaccgggc cagccgcccc
     2521 cgaatccacg cccccgtcga ctcccccgcc cctgcgcccg ccggtacgac tgcaccgagg
     2581 caaaaccacc cacaaggaaa aacaggccca gcatctgcaa cacccaggtg atgggcgcca
     2641 gaggggcaaa actcgacaac ggactctcgt tgtgcaacgc gccgtccgca tcccgcgcga
     2701 gccccccgat aagccagtgc ccggtcggca cggcaagcaa cgccagcgcc cgcaaaccgt
     2761 caatagcacg atcccgctgc cccggagcaa catttccacc gctactgatg acacctgacg
     2821 gcagcattct cggccgaagt cgttcaggta gactcacgcg ttcgcttttc tcttcggaag
     2881 cggcatgccg acaccccgct catcacccga gcagaagccg gcaactcgac cactcaatgc
     2941 tcagccgaag cgaaaaatcc cacagtaagg ccagcgcccc aaccatggtg gtgctgactg
     3001 cacttccccc cagggggcca ccccccgctc ccacgggggc ctgccgcagt gacatgcagc
     3061 accacccggc cacgctccat acgcacaagg acccacatcg gacccggagc aagacaccga
     3121 agcgggcgcg gctgccactc ggccgctggt ctctacgggg tgcggggctc caggcggtag
     3181 gtgaaagcgg cagtgccggg gtcgagggct gttgcgccgc cgtcgacggt cagtaccgcg
     3241 ccgttgacga aggacgcggc gggggagagc agccaggtga tcgcctcggc gacctcccgt
     3301 gggtcaccgg ggcggcgcgc gggaagcagt cgggtcgcct cggtgtacgc ggcctcggtg
     3361 ccaccgtccc cgagccccgc ctcctcggcg aagcgcgcca tccggcgatc ggccatatca
     3421 gtacgtaccc agctagggca caccgtgttg gcgcgcagcc cctgtggtcc gtagtcgacg
     3481 gccagggagc ggcagagctg gagcaaggcc gccttggagg tcgcgtacgc ggcattgccg
     3541 acaccgttgc gcagcgcgga caccgaggcc accgcgacga ccgaaccgcg cgacttcagc
     3601 aagtgcggca gcgcggcgcg caggagcagg aagggaccgg tgaggttgac gcgcatcacg
     3661 gcctcccagt cctcgtccga cagctcgccc acggtgccgc tgcgcccgat gcccgcattg
     3721 agtacgaggc cgtccaggcg gccatacgtg gcgacggcgg tatccaccag gtcgcgtacc
     3781 gcatcgggat caccgatgtc ggaaggatgg gccagggcgc cggtctcctc ggctatatgg
     3841 tgcagcggct ccggccgccg accggagacc accacctgat ggcccgcttc ccgcagcagc
     3901 cgggcggtgg cggcaccgat tcccgttccg ccgccggtga cgatgactgc ttgttgttca
     3961 gacatgggtc ctcaacctcc gagacgaccg gatgcgagct gtgtgatcag acgtatatac
     4021 ccagggatgt cggccgcacc cgatcgacgc gacccgcgcc cggcccgcgc gccgacagag
     4081 agcggagcga agcgagcggc agccgacgcc ccgagcccgg gaggggggtc cgcaccacgc
     4141 ccatagatcg tgtagtgttc tccgagttgc cgcggcgccg accggcccgg ggcagccact
     4201 cacgccgcac cggcggcaca ccactactgc acggcccctg aacgggatga atttcggcat
     4261 gccggaattc gaaaccggac tcgattatga gtcgccaggg aaatccgcta aagtggtgga
     4321 cacgccggaa ggcgcgaaac accccctccg acaggtaatc ggaaccgaat tcgaaccgga
     4381 aacggaacga aatgaggatc tgctagagtc ggaaccgcgg aaaagccgaa aggccggacc
     4441 gcaccggcgg aaatcaggcc cggaaggatc tgatagagtc ggaaacgcaa gaccgaaggg
     4501 aagcgcccgg agggcccggt gaaacgggac cgaaggaagc gtccgttcct tgagaactca
     4561 acagcgtgcc aaaagtcaac gccagatatg ttgatacccc gtccaccggc ttccggccgg
     4621 aggatgaggt tcctttgaaa agcccaccgg caccgatggt gcgggtggca cacacagcga
     4681 ggacgctgtg aacgggcggc ttattccgct gcctgttccg ctctcgtgtg tgttgacccg
     4741 atcacgggta aacattcacg gagagtttga tcctggctca ggacgaacgc tggcggcgtg
     4801 cttaacacat gcaagtcgaa cgatgaacct ccttcgggag gggattagtg gcgaacgggt
     4861 gagtaacacg tgggcaatct gccctgcact ctgggacaag ccctggaaac ggggtctaat
     4921 accggatacg acacgggacc gcatggtctc cgtgtggaaa gctccggcgg tgcaggatga
     4981 gcccgcggcc tatcagcttg ttggtggggt gatggcctac caaggcgacg acgggtagcc
     5041 ggcctgagag ggcgaccggc cacactggga ctgagacacg gcccagactc ctacgggagg
     5101 cagcagtggg gaatattgca caatgggcgc aagcctgatg cagcgacgcc gcgtgaggga
     5161 tgacggcctt cgggttgtaa acctctttca gcagggaaga agcgcaagtg acggtacctg
     5221 cagaagaagc gccggctaac tacgtgccag cagccgcggt aatacgtagg gcgcaagcgt
     5281 tgtccggaat tattgggcgt aaagagctcg taggcggctt gtcgcgtcgg atgtgaaagc
     5341 ccggggctta accccgggtc tgcattcgat acgggcaggc tagagttcgg taggggagat
     5401 cggaattcct ggtgtagcgg tgaaatgcgc agatatcagg aggaacaccg gtggcgaagg
     5461 cggatctctg ggccgatact gacgctgagg agcgaaagcg tggggagcga acaggattag
     5521 ataccctggt agtccacgcc gtaaacgttg ggaactaggt gtgggcgaca ttccacgtcg
     5581 tccgtgccgc agctaacgca ttaagttccc cgcctgggga gtacggccgc aaggctaaaa
     5641 ctcaaaggaa ttgacggggg cccgcacaag cggcggagca tgtggcttaa ttcgacgcaa
     5701 cgcgaagaac cttaccaagg cttgacatac accggaaaca tccagagatg ggtgccccct
     5761 tgtggtcggt gtacaggtgg tgcatggctg tcgtcagctc gtgtcgtgag atgttgggtt
     5821 aagtcccgca acgagcgcaa cccttgttct gtgttgccag catgcctttc ggggtgatgg
     5881 ggactcacag gagactgccg gggtcaactc ggaggaaggt ggggacgacg tcaagtcatc
     5941 atgcccctta tgtcttgggc tgcacacgtg ctacaatggc cggtacaatg agctgcgata
     6001 ccgtgaggtg gagcgaatct caaaaagccg gtctcagttc ggattggggt ctgcaactcg
     6061 accccatgaa gtcggagtcg ctagtaatcg cagatcagca ttgctgcggt gaatacgttc
     6121 ccgggccttg tacacaccgc ccgtcacgtc acgaaagtcg gtaacacccg aagccggtgg
     6181 cccaacccct tgtgggaggg aatcgtcgaa ggtgggactg gcgattggga cgaagtcgta
     6241 acaaggtagc cgtaccggaa ggtgcggctg gatcacctcc tttctaagga gcacttctta
     6301 ccggtccttg gggctggtca gaggccagta catcagcgag tgtctgatgc tggttgctca
     6361 tgggtggaac gttgactact cggcacagtc ggttgatgga tcgttagtac tgcttcggcg
     6421 tggaacgcgg tgggtcggct ggttgtgtcg ggcacgctgt tgggtgtctg agggcatgga
     6481 cgagagtctg taccttcaaa cgccggcccc ggtgaagcat cacgtagtgg tgtgtgacgg
     6541 gtggctggtc gttgcttgag aactgcacag tggacgcgag catctgtggc caagttttta
     6601 agggcgcacg gtggatgcct tggcaccagg aaccgatgaa ggacgtggga ggccgcgata
     6661 ggccccgggg agctgtcaac cgagctttga tccgggggtg tccgaatggg gaaacccggc
     6721 agtcgtcatg ggctgtcacc catacctgaa cacatagggt atgtggaggg aacgcgggga
     6781 agtgaaacat ctcagtaccc gcaggaagag aaaacaaccg tgattccggg agtagtggcg
     6841 agcgaaaccg gatgaggcca aaccagttac gtgtgatacc cggcaggggt tgcgtggctg
     6901 gggttgtggg atcgcacttt catcgtctgc cggcggtgag gtgagtcaga aattgctgat
     6961 gtaggcgaag gacatgcgaa aggtccggcg tagagggtaa gacccccgta gctgaaatgt
     7021 cagtaacttg cttgtgcgac acccaagtag cacggggccc gtgaaatccc gtgtgaatct
     7081 ggcgggacca cccgctaagc ctaaatattc cctggtgacc gatagcggat agtaccgtga
     7141 gggaatggtg aaaagtaccg cgggagcgga gtgaaatagt acctgaaacc gtgtgcctac
     7201 aagccgtggg agcgtcgcat gcgtgcttgc acgtatgtcg tgactgcgtg ccttttgaag
     7261 aatgagcctg cgagtttgcg gtatgttgcg aggttaaccc gggtggggta gccgtagcga
     7321 aagcgagtcc gaagagggcg ttgagtagcg tgcccaagac ccgaagcgga gtgatctagc
     7381 catgggcagg ttgaagcgga ggtaagactt cgtggaggac cgaacccacc agggttgaaa
     7441 acctggggga tgacctgtgg ttaggggtga aaggccaatc aaactccgtg atagctggtt
     7501 ctccccgaaa tgcatttagg tgcagcgtcg tgtgtttctt gccggaggta gagcactgga
     7561 taggcgatgg gccctaccgg gttactgacc ttagccaaac tccgaatgcc ggtaagtgag
     7621 agcgcggcag tgagactgtg ggggataagc tccatggtcg agagggaaac agcccagagc
     7681 atcgactaag gcccctaagc gtgtgctaag tgggaaagga tgtggagtcg cagagacaac
     7741 caggaggttg gcttagaagc agccaccctt gaaagagtgc gtaatagctc actggtcaag
     7801 tgattccgcg ccgacaatgt agcggggctc aagcacaccg ccgaagtcgt gtcattgcgg
     7861 tattacccct aacggggatc gtgatgggta ggggagcgtc gtgtgccggg tgaagcagca
     7921 ctggaaggta gttgtggacg gttcacgagt gagaatgcag gcatgagtag cgatacacac
     7981 gtgggaaacg tgtgcgccga ttgactaagg gttcctgggt caagctgatc tgcccagggt
     8041 aagtcgggac ctaaggcgag gccgacaggc gtagtcgatg gacaaccggt tgatattccg
     8101 gtacccgctt tgaagcgcca aacatcgaat caggcgatgc taagtccgtg aagccgccct
     8161 gagctcttcg gagtgtaggg gagtggtgga gccgacggac cagacttgta gtaggtgagt
     8221 gatggggtga cgcaggaagg tagtccagcc cgggcggtgg ttgtcccggg gtaagggtgt
     8281 aggccgtgcg gtaggcaaat ccgtcgcacg ttaaggctga gacctgatgc cgagccgatt
     8341 gtggtgaagt ggatgatcct atgctgtcga gaaaagcctc tagcgagttt catggcggcc
     8401 cgtaccctaa accgactcag gtggtcaggt agagaatacc gaggcgttcg ggtgaactat
     8461 ggttaaggaa ctcggcaaaa tgcccccgta acttcgggag aaggggggcc attgctggtg
     8521 aggggtcttg catcctgagc tggtggtggc cgcagagacc agcgagaagc gactgtttac
     8581 taaaaacaca ggtccgtgcg aagccgtaag gcgatgtata cggactgacg cctgcccggt
     8641 gctggaacgt taaggggacc ggttagtgac ctttcggggt tgcgaagctg agaacttaag
     8701 cgccagtaaa cggcggtggt aactataacc atcctaaggt agcgaaattc cttgtcgggt
     8761 aagttccgac ctgcacgaat ggcgtaacga cttctcgact gtctcaacca taggcccggt
     8821 gaaattgcac tacgagtaaa gatgctcgtt tcgcgcagca ggacggaaag accccgggac
     8881 ctttactata gcttgatatt ggtgttcggt tcggcttgtg taggataggt gggagactgt
     8941 gatctcgtca cgccagtggc gggggagtcg ttgttgaaat accactctgg tcgtgctgga
     9001 tgtctaacct gggtccgtga tccggatcag ggacagtgtc tggtgggtag tttaactggg
     9061 gcggttgcct cctaaagggt aacggaggcg cccaaaggtt ccctcagcct ggttggcaat
     9121 caggtgttga gtgtaagtgc acaagggagc ttgactgtga gactgacggg tcgagcaggt
     9181 acgaaagtag ggactagtga tccggcggtg gcttgtggaa gcgccgtcgc tcaacggata
     9241 aaaggtaccc cggggataac aggctgatct tccccaagag tccatatcga cgggatggtt
     9301 tggcacctcg atgtcggctc gtcgcatcct ggggctggag tcggtcccaa gggttgggct
     9361 gttcgcccat taaagcggta cgcgagctgg gtttagaacg tcgtgagaca gttcggtccc
     9421 tatccgctgt gcgcgtagga gtcttgagaa gggctgtccc tagtacgaga ggaccgggac
     9481 ggacggacct ctggtgtgcc agttgttctg ccaagggcat ggctggttgg ctacgttcgg
     9541 aaaggataac cgctgaaagc atctaagcgg gaagcctgct tcgagatgag ggctcccacc
     9601 tcctttgagg ggttaaggct cccagtagac gactgggttg ataggccaga tatggaagcg
     9661 ccgtaaggtg tggagttgac tggtactaat aggccgaggg cttgtcctca gttgctcgcg
     9721 tccactgtgt aggttctgaa gtaacgaact cgccttgctg gccggagttg ttatcttcat
     9781 agtgtttcgg tggtcatagc gttagggaaa cgcccggtta cattccgaac ccggaagcta
     9841 agcctttcag cgccgatggt actgcagggg ggaccctgtg ggagagtagg acaccgccga
     9901 acaaatttta gagaaagccc cgtagggaac ctggttccct acggggcttt ttcgcgtttt
     9961 tggaagctgt aagccagtcc tgtgcattgc tgagcccggg tcatgaagcc ggtcggcggc
    10021 gcgggagatg gtaaagggtg tgcagggctg ccaggcgggt ggcgcgaagg atgcgcaacg
    10081 gcgcgtagac gtctttgtcg tgccacagag ccgggatgtt gtcggtgacg cactcggcct
    10141 ggaggatacg gcgggcggag gtcagggcct gccgcgcggc tccggtctgc ggcgtacggc
    10201 ccagcaggat ccacaccgcg tacgcggtct cctccacggt cccgtttgcg ccgtggcccc
    10261 agcgtccacc gggcagacgg gtggccagcg cccaggccga cgcgcgggcc agagctggat
    10321 gggcgaccgg cccggcgaat gcggccagcg cacggacaca gcaagcggtg gcgtagtagg
    10381 gggaggcgtg ccacttgtcg ctccaggagc cgtcgggatg ctgctgggcg agcagccagt
    10441 cgatggtccg acggatgacg gaccggtagc gcccgcggtc ggcggggtga cgcgccacgt
    10501 acaggacaag agcctccagg gcgtgggcgt tggtgctgga ggagggggac cgttcctcgg
    10561 ggtagcagca gaagtggtcg tcggtgaagt acggcatgag actgtcgggg cgtatgtggt
    10621 cgccgtggtg caggagggcg tagagaacgg cagcggtgtc gtcggcgtcg gggggaagac
    10681 cggggctgcc gggagcgccg gcctcaccca gcgcatcgcg cagaccggcg aggagcgcgg
    10741 gcggcggggt gtgcgggacg ccgtgatggg cgaagagccc tgcgatccag gcgcgttcga
    10801 aggtggtgat gggggcggcg acggggaccg ggccaccgta gcggctctgt atacggtgca
    10861 ggtagcggag gccggcctgt cgccgctcgc tgtcgtgtgg ccggcccagc caggcggcgg
    10921 tggcagccgg ggaacagctc acggccccct cgtacggacg gatgtgacgg tcggggtgtt
    10981 cggtggccgg ggcgaggatc tcccaggtgt gccagagctt ttccggtagt ggttctccct
    11041 ggtagaccgc agcgcgcagc tcggccagga ccgaaggaag gcaccctttc ggccgggcga
    11101 gaagtatgga ggaggcccag ggcagaagaa agggtggccg actgcgtgcg cagcggccca
    11161 ggtgctggtg gatccggtcg atcagggcgg gaacgatgag ttcgaccgcg acggtgtcgg
    11221 gcagcgtcgg ggggcactgg tggttgccga gcgagtgact gaggcgggtg acggcgagtg
    11281 cggtggtgtt gaccagttgg gggcggggga cgtggacatc gtcgccgcgg gcgaggacgg
    11341 tgagcagtgc ctcggccgcg ctgagggtct gcagcagtcc gtgttcggcg gtgccccagg
    11401 tgccgtcggg gttctgacgc tcgcaaagaa agcggacccg ccggctgtgg ccctccagcc
    11461 acggggcgtc ggacaccagg cgggcggtct cgtacaccga tggtgtgctc tgcccgaggt
    11521 cgtcggcggt tgcctcgttc agcagcttgg ctgcatgcag gtcaaaggga gtgtggttct
    11581 tcacggtgga cttccgttcc gggcagttcg aaaagcaggc atgcgaccga gcggcggaaa
    11641 cacggctcgg cacccggtag ccgtgggagc actctggtga tgcggaggta tttcgctccg
    11701 gtgcttgtcc ggtgccgcct cctgctgtcc tatggggaca gcaggaggcg ggaaggttca
    11761 ccggaatcat cggtagaagt gattggcttc gtaccagccg ttggaactca ccagtactgc
    11821 cattccctgg gcgaaagccc tcagttgggg gtcgggcgaa gccgcgagct cgtccgacag
    11881 gcggcggacc cgggcgatct ctgcgtcgag gatcgccacg gaccggtcga ctgcctcgtc
    11941 caggccgtat gcatggtgcg cggcgagtac gccgggcagg gagaacggca gccctccgcg
    12001 aacgtcgtgt tccctgaagg cggagtagat gtcgttcacc cagccgatgt cgttggccac
    12061 agccatccgc aggtcgtgaa cggtcgggga atcgcgtacc tcggggggca ggtcgcattc
    12121 catggccagc tcggcgaaga ggacgctgat gtgtccgtag accacatgcc tacggagcac
    12181 cgtgaagtct tcgagtgtga cgtcggccgg gtcctccagc agggcttcac agagcattgc
    12241 ggcgaaggcg gcgtggtacg ccatcacgac gcgctctgtc atgcgaccac ttgcggtggc
    12301 gcggagccgg gccacgaaag cagccagggc cgaggcgaag ccgggctcgg tgggacgggc
    12361 accgggttcc agcatgcgca tcatggtggt tatgcgtcgt gcgaagcccg gcatatcggc
    12421 tcgggcgcgt tcctcgcagt ggacgtcgtc gaatacggtc agccaccaca tgccctcccc
    12481 gatgatctcc gccatgaccg ggtcggtgac tttccaccac cccagatgga tccccagggc
    12541 cagacgtgag cggtattccc tgtcacggac caccgaattt tccatgaggt ggtgctcttc
    12601 cgcccaggcg cggatgtgcc gttccacggc atgtccgatc cgggtgaatt cgggaggctg
    12661 tgcagcccat actcccgcca gttggggaaa atgccgctgc aggctttcgt acatcggtgt
    12721 tcggtcggtg ccgtcattga ccttgttcca ggccctgacc ggttcggtgg tcatcgtggg
    12781 tgcctttcgg agcgggctgc ggcgcatgct ctgtgctgtc tgtttcgggt ttcttgtccg
    12841 ccgtgggtga cgggggctca gaggggggaa gaggcgtggc aggccggcag gcaggtggcg
    12901 tgccgggagt cacggaggag gcaggcagag acggagatgg tggggcggag gaagcagcac
    12961 ggccctcgtc aggaccggtc gggcgagcgg cggacgacgc gcgttgtggt gtcagcgttg
    13021 aagaagccgg cgaagacgcg gtgagcgatc tgtttgtggc tgcggcgggt gggggctgca
    13081 ccggttggtt gggaggttgg ttgggagatg gccagggtga cgccatcgcg ggtgggggga
    13141 cgatgcgtac gtcggcggtt atggggggtg tggtggcgca ggctgcggcg gtgaggaggg
    13201 tgcacagggc gagggccagg agcagggttc gcatggggga gccttccgtt ggggattggc
    13261 ttgactgaat ggcgagtcag gaggcgctga gggtttctac gaattttttg aggccggtga
    13321 agaatttggt gatgccgtgg gccatgtcca ggagagtgtg ggcggtctgg acgggggcta
    13381 tggctacgag aaagaggaag gtgatcagga ccagccagaa gagcagtcct cggggttggc
    13441 tcagcacggc ggtgtctccc tccggtgggg ttcggccgcg ggcgtgcgcg tatcaggtgg
    13501 gtggctgatg gtggcggtgg cgtctccacc aggtgagggc gaggagtacg gcgagggagt
    13561 cgcggaggac tttgtggttg ctgagtggga ggttttgtag ggcgcggttc atggggagct
    13621 ggttgacctg gacggtgagt tcgcgattcg acaggtcgcg gatgatgctg cgggtctgct
    13681 ggggtgcggt ggcgctgccg aggatgaggt cgagggcggt gcgggcggcc tgtttctcgg
    13741 agagggcttc ggtggcgagg cggaaggcga tgtgccggaa ttcctcttcg aagatgtcga
    13801 tgagggacag ttcggggcac aggttgcgga tggagccttc gaggttggcg aaggacttgc
    13861 ccagtacgga gatcatgggg ctggttttga tgccgcgttg ggtggagtgt tccaggatgg
    13921 cggtgagggt gacgccgaag ttgagttctt ccagggtggc ggtggcgatc tgggggacga
    13981 gggcggccat gtcctcgatg aagtcggtga cttcggccca gggagtggcg tggcccatct
    14041 cgatccaggc tttggcggtg gcggcgccgt cgttctgggc gaggctgagc aggaccagca
    14101 ggatgctggt gctcaagggg cggtcgatgc ggccgaccat gccccagtcg atgaggtggg
    14161 cgggctggcc ggggtggacg aggatgttgc cggggtgggg gtcggcgtgg aaggtgcggg
    14221 tcaggaagta ggagcggtac atgaaggcga gcaggtcccg cccgatgccc gcgcgttcct
    14281 ccagagaaaa gtcgtggggg cgggcgtcgg cgatggagca gcccggggcc aggctctgga
    14341 tcaggacgcg gggggtggcg gagatcgggg tggggacggt gaggtgtttg aagtccgcgg
    14401 tgtgtgtgcg ggcttggtgc atgttgcggg cttcgagggt gaagtcgagt tcggggcgca
    14461 tggcgtcgaa gacgacgccg agcatggcgc tcacatcgat gacggcgttg aagcgggggg
    14521 actggcgggc gaggaagcgg gccgccttgg tcagcagggc catgtcggct tcgaccaggg
    14581 ggcgtacgcc ggggcgctgg atcttcaggg ccaccggggt gccgtcggcg agggtggcgg
    14641 cgtagacctg ggccagggag gcggtgccca gggggcggtc ggtgtcgatg tcgcggaagc
    14701 cggctgccca cagtgggccg aggtcgccgg ccatgaccgg ttcgaagtcg gcgaaggggg
    14761 acgggctgac ctggtcgtgc agcgtctgca gttcctcgat cagggtgggg gagaccaggt
    14821 cgggacgggt ggagaggatc tggccgacct tgatgtagaa ggggcccagg ctctccaggg
    14881 cctcgcgcag cgcgcgggcc cggtcacgtg cggcactctc accgtcggcg tcgcggtgcc
    14941 gggtgtgccg ggcgagggtg tgcgcgccct cgcggacgag cagggtgccg aacgtggtga
    15001 ggaccaggcg ggcacggccg gttgccatgg gcagctccaa taaaggcgcg ggaggccggg
    15061 gtgcggggct cagcgtcctc cggtgggcgt ggcaccgcgg cgtggcgggt cgcccagcag
    15121 cgtggccgga ttcagggggc tgtgggctgc cgcggtgggg acccacgccc cgagtgccca
    15181 ggcgatcacc tcggccagta cctggtacgt acagtcctcg ccggtccggt ggtcgcgtac
    15241 gacgaactgg cggctcgcac gcaggtcctg gatcagttcg tcgagggtga cgggttcgtg
    15301 cagccgtacg tcgatgagcc ggccgccgcc gtgccggttc aggacgcggt cgggcggcgg
    15361 ccccggccgg tgccggtgcc ggcgcggcga accggctccg gccgggggca ggcggccggg
    15421 accgcccggc accgtcacga cgacggctgc ttggcgcgcc ccgcgggctg ggcggcgacg
    15481 gtcccctcct gctgctggcg cagcagctcc tcgatctgcc cgagcttgga ccacagggcc
    15541 gccatctcca gttgcgggct ccgtgcctcg tccgggcccg tacggcgccg ctcggtgtcc
    15601 cgctcggtgt cccgttcgcg ctccgggcgc aggctcctgc tcagcgagtc gcgggtgtcg
    15661 tgctccaggt cgcagacgct gtcgagggcg tcgtcgagca tgtccttgat gtcgtcgacg
    15721 atgttcgaga cggtcttggt gatgccggta cggcgggcca tggtgtctgc tccttctggt
    15781 tctctacggg ggtccggtgt ggcgcgggtg gcggtcggcc gtcaggccag tggcgaggcc
    15841 ggtggtcagg ccggtgcgtc cgggggcggc ggcccggtgg tccggggcac gaggttgcgg
    15901 ggcagggcga aaagctggtg ctcctcggcc gcccgtacca cgtcgatgcg tgtgaatccg
    15961 tacgtggcga gccggtccag gacgccgtcg acgaggaggt cgggaaccga ggcgccggag
    16021 gtgacaccga ccgtccgtac gccggtcagc cagccctcgt cgatgtcctc ggcggtgtcc
    16081 accaggcggg ccgcgcccgc gccggcgtcc agggcgacct ccacgagccg tacggagttg
    16141 gaggagttgc gggagccgac gacgaggacc aggtcggcgt cggcacccag ccgtttgacc
    16201 gccgcctggc ggttctgtgt ggcgtaacag atgtcgtcgc cgggcgggga gagcaggtgc
    16261 gggaagcggg cccggagggc ggcgaccgtg gccgtcgtct cgtcgacgga cagcgtggtc
    16321 tgggacagcc aggcgacctt ctccgggtcg cggacctcga cggtcgcggc gtcgtcgggg
    16381 ccgtcgacca cggtgatgtg gtcgggggcc tggccgctgg tgccgatgac ctcctcgtgc
    16441 ccggcgtggc cgatcagcag gatgtcgtaa tcctcgcggg cgaaccgtag ggcctccttg
    16501 tggaccttgg tcaccagcgg gcaggtcgcg tcgatggtgg tcagacggcg ggcggcggcc
    16561 tcctcatgga cgccgggggc gaccccgtgg gcggagagga tgacggtggc gccttccggg
    16621 acctcggtgg cctcgtcgac gaagacggcg ccccggtgct ccagggactt cacgacgtac
    16681 ttgttgtgga cgatctcgtg gcgtacgtag acgggcgcgc cgtacagctc caaagccttc
    16741 tcgacgacgg tgacggcgcg ggcgacgccg gcgcagtagc cgcggggagc cgccagcagt
    16801 acccggcgcc gggcggtcgg tgtgctcata gggcacgctc cgtgagggtg tgggcgacct
    16861 cgcgcagcgc gtcccgggcg tcggcgggga agggcgcgca gtccaaggcg cgcagagcgg
    16921 tgtgcaggtg ggagtcgatg agcgtctcga cccgggcgcg ggcaccggtg ccggccagga
    16981 cggtacgcaa ggtggcggcc tgttcctcgt ccaggtccgg gtcgccgacc aggtggcgca
    17041 gcgtccgcgc ctggcgggcg tcggcgtggc gcagggccag ggcgagcagg acggtgcgtt
    17101 tgccttcccg caggtcgtcc aggacggatt tgccggtgac ggcggggtcg ccgaagacgc
    17161 ccagcaggtc gtcgcgcagt tggaacgcct cgcccagcgg cagggcgtag gcgctcagcg
    17221 cggcccgtac ggcaggcccc gaaccggcca gggcggcacc caggtgcagc gggtgctgga
    17281 cggtgtactt ggccgtcttg tagcggacga cggccagcgc ccgctccacg tcgtcggtca
    17341 gttgttcggc ggtgtccagg tccatgtact ggccgtagag caccgcggtg cgcatggcgt
    17401 ccagcgtggg cagggcgacg gtgagctggg ccgtgctcag acccgcgccg ttgagcatct
    17461 cctgggagca ggccagcgcc aggtcgccca gcaggatggc ggcgtgctca ccgaactcga
    17521 tgtgatgcgt acggtgttga tggcgctcgg caagggtacg gtgaacggtg gggtggccgc
    17581 ggcgggtgtc gctgcggtcc atgatgtcgt cgtggatcag ggcgaaggcg tggaagagct
    17641 ccagggaggc cgccgcccgg agcaccgggt cggcctcgcc ccgcccgcgg gcggcgtacc
    17701 agcccaggac gcacatcagc gggcggatgc gcttgccacc ggcgaacagg aagtcgcgca
    17761 ggacctccag cgggagcgcg ggcaggtttt cggtgcgcgc ggcgtacgcc ttgacggtca
    17821 ggtagtggtc caggcaggaa tcgatccgct tgcgcaggag ggggaggtcc agcggcgttt
    17881 ccaaagcggc ggtggtcatg acgccggctc cggttcctgg tttccgccct gcccggcccg
    17941 gggctgcccg gtctcggtcc gtccggcctg ggcctgttcc acacgagact gttcggcctg
    18001 ggcctgttcc acacgggcct gttcggcctg ggcttcgccg aggcgcaggg cctcttcgac
    18061 gagagcctcg acgatcttcg attcgggtac ggtcttgatg acggcgccct tacggaagat
    18121 ctggcccttg ccgttgccgg acgcaacgcc gagatcggcc tcgcgggcct cgccggggcc
    18181 gttgaccacg cagcccatca ccgccacccg cagcgggtgt gggaagccgt ccaaagccgc
    18241 ggccacccgt tcggtgagcc ggtagacatc gacctgggca cggccgcagg aggggcagga
    18301 gacgatctcc agttgccgtc ggcgcaggcc cagggactgc aggatctgca ggccggcctt
    18361 gacctcttcg accggcggcg cggtcagcga gacccggatg gtgtcgccga tcccctcggc
    18421 caggaggatg gcgaaggcgg tggcggattt gatggtgccc tgcgggggtg gtccggcctc
    18481 ggtcaccccc aggtgcagcg ggtagtcgca ggcggcggcg agccggcggt aggcggcgac
    18541 cgtcgtcagc gggtcgtggt gcttgacgga gatcttcagg tcggtgaagc cgtgctcctc
    18601 gaacagcgag cactcccaca gcgcggattc caccagggcc tgagggctgg ccttgccgta
    18661 cttggccagc agccgccggt ccagcgagcc ggcgttgaca ccgatccgga tcggcacttt
    18721 cgcgtcggag gcggcccggg cgatctcctt gaccttgtcg tcgaacttct tgatgttgcc
    18781 ggggttgacg cggaccgccg cacacccggc gtcgatggcg gcgaagacgt actgcggctg
    18841 gaagtggatg tccgcgatca ccgggatcct cgacttggcg gcgatggcgg gcagcgcgtc
    18901 cgcgtcgtcc tgggagggca ccgccacccg tacgatctgg cagccggccg cggtcagttc
    18961 ggcgatctgc tgcaaggtgg cgtcgatgtc ggtggtgggg gtggtggtca tcgactggac
    19021 ggagaccggg gcgccgccgc ccaccgggac atcgccgacg tggagctggc gggagcggcg
    19081 gcgctccggg gtctggggag gtgcggcggc cggggacggg ggccggggcg ggtatggggg
    19141 tgagccggcc ggccgggcgc ggacctcggg tatgggcagg ttggtggtgg tcatcgaagc
    19201 ctccgggggt ggtgcgcgtc atcaagggcg gggagcggga cgtacggggc ggtgggcgcg
    19261 tacggagtgg cgggcgggta cgtacgggac agtgagtgcg tatggagcgg tgggtgcgta
    19321 cggagcggtg ggcgcgtacg gacccttcag catgtacgga ccgttcggcg cgtacgggac
    19381 ggcgggtgcg tcatcggcgc cggtgccggg cgttccaggc caggtggacg gcggtggcga
    19441 tgccgccggc gtccaggccc gtggcagcca gcagttcggc gcgcgaaccg tgggtcagaa
    19501 aggtccgggg cagacccaga ctgtgtacgg gggcggcgac cgcggcgtcg gtgcaggcct
    19561 gggccagcgc ggagcccacc ccgccggtac gggcgcagtc ctccaccgtg acgaccagcg
    19621 tgtgacgggc ggcgagctgc accagctccg gcggcagcgg tagcacccag cgcggcgcgg
    19681 cgacggtggc ctccacaccg cgcgcggcca gccgttcccg ggcggccagg cagggagcgg
    19741 ccatcgcccc cagacccacc agcagcacgt cgcgcccggg agccgagagc agatccacct
    19801 gccccatacg gcccaccgcc ggtacgtccg cgcccaccgc tcccttgggg aaacgcaggg
    19861 cggtggggcc gtcctggtcg gccagcgcct ctcccagcag ggtgcgcagc gtggtggcgt
    19921 cccgtggggc ggccagccgc aggccgggga ccagggagag cagggtgtgg tcccacatgc
    19981 cgtgatggga ggggccgtcc gggccggtga tgccggcccg gtccaggacc agggtgaccg
    20041 gcagccggtg cagggcgacg tccatcagca actgatcgaa ggcgcggttg gcgaaggtgg
    20101 cgtagagggc gacgaccggg tgcaggccgc ccatggacag cccggcggcc gagcacatcg
    20161 cctgctgttc ggcgatgccc acatcgaaga cccgccccgg gaagcggtcg gcgaacggcc
    20221 ccagcccggt cgggcgcagc atcgccgcgc acaccgccac cacgtcctcg cggccggcgc
    20281 ccagctccgc cagctcgccg gcgaagacat cggtccaggt gggcgcagcg gtgtcgcggg
    20341 cggtaccggt ggccgggtcc acgacgccca ccgcgtgcag gcagtccgcg tcatcggtct
    20401 ccgccggtac gtagcccttg cccttgacgg tgacggtgtg caccagcacc gggccgccga
    20461 tttcgcgcgc ctgccgcagc gccgcctcca ccgcgtccag gtcgtggccg tccaccggac
    20521 ccagataggt gaatcccagg caggtgaaga ggttgcgcgc ctcgggcggg ccgtccacat
    20581 aggcgcttac gggggcatcg gcctgggcca acggccgcct cagggcgctc aggtgatcgg
    20641 ccagtgcccc ggtggtgggc gcgtaggaac ggccgttgtc gttgagcacc accgcgatac
    20701 ggcggcgccc ggccgtcccc agattgttca gcgcctccca ggccagcccg ccggtcaacg
    20761 cgccgtcccc gatcaccgga acgacccacc ggtcgccttc gccccttgcc gcgaaggctt
    20821 ttcccagacc gtccgcgtac gccaggctgg tggaggcatg ggagttctcc acccagtcgt
    20881 gcaccgattc gctgcggctg ggatatccgg acaacccgcc ccgctgccgc agccggtcga
    20941 agccgtgcgc acgtccggtg atgattttgt gcacgtacgc ctggtgcccg gtgtcgaaga
    21001 tcagggcgtc gcggggcgag tcgaaaaccc ggtgcaacgc cagagtcagc tccaccgcac
    21061 ccaaattggg gccgaggtgg ccgccggtcc cgcagacgtt ctcgatgagg aaggttcgta
    21121 tccgttcggc caccgcgacc gtttcgcgcg gcgataacgc ccgcagggcc tgcggtgacc
    21181 accgtatggc agccatggca gtgtcaccgt cgtcatcggc agccggtgga agccgcgtcg
    21241 gactcggggc aagacgcatg gatttccccc tgagcactgg ttatccggga acggacgtct
    21301 gatgggcggc gggcaccgcc cacatgcaca tccctgcacg cagggaggaa ccatccggcg
    21361 tcgaaaagac gattcccccc tggcgtcggc cgccggcccc gcggcccccg agcgatctcg
    21421 catcgctcct tccgcggccc tgccgagcag ccacttcgag cccaccgcaa accgccgccg
    21481 ccggcaaccc cgtacggacg gcgcgcggag tgacgccctt cagcacttcc cgggcgtacg
    21541 cgccggccca ccgagcacac gccatcaggt ccgctcaccc caggcgcaca cccggaccgc
    21601 ggcggtccgc gccggtgggc ggtcgctgtt ccggccctgc ctcgtacggt gttccggcag
    21661 ctcaggacct ggccggacca cgacggccag ggcatcttca ggcatccccg gagcatcccc
    21721 gggacaccac cctgttcctc cacccactcc agacacccgc tgtcgcacat cgccgcgcac
    21781 ggtgatgccg cgcgctcagc attgcgcgcg tgcgacgacc gtcagcagac cgttcacttc
    21841 tgcgcgccct tggcggaatc acgcgacccg ccggtgtccc cgggccttga ggggccgggg
    21901 tgctccggag tgctcggagg tgctccggga gagtcgggta gtccggcgtg gtccgggcca
    21961 aggggcgtat atcagccacc ccttgatcac gaccggttac gccccctagc atcgagaacg
    22021 gtcatgcgcg gccgatcgcc ggcggtgcgc tggaggagtt gcccggctgc ccgtacggcc
    22081 ggccgttcgg ccatgaccgg gccccgggga ccggtggcga tacgaagccg gtcgacgcca
    22141 tgcgccggcc cgcgttcagc gacacctgga accctggcgg gccgccgatg cccgccatca
    22201 acgaggaggc cgtcccatgg aatccgtcgc gctgatcacc ggcggttcga ccggaatcgg
    22261 tgccgccacc gcccgcgccc tgctcaagca gggccaccgt gtggccgtca ccggacgcga
    22321 cgccggccgc ctggctgcct tcgccgcctc cgccggagcg ggggagcaac tgctgacgtt
    22381 caccggtgac gccagtgacg cgagccatgt cgcctccgcc gtacgtcagg tcgtcgacac
    22441 ctggggccgg ttggacaccg tcatcgccaa cgccgggttc tccctgcccg gcaccctgga
    22501 ggaccaggac cccgaggcga tgcgtgccat ggtcctcacc aatgtcctcg gcccgtccct
    22561 gctggtacgg gagacactgc cgcacctcag ggagtccaag ggccggatcg tgatcgtcgg
    22621 ttcggtcgcc ggcgtcaggc acacgcccgg caacctgtac tcggtcacca agtgggccgc
    22681 gcacgccctc gccgagaaca cccggctcct ggtcaccaag gacggagtcg gcgtcaccct
    22741 cgtcgccccc gggatcgtgg acaccccgtt ctgggacaac cgtggcggac gccccgatac
    22801 gtcaccggcg atgaccgccg agcagatcgc cgaggccatc ctcttcgccg tcaaccagcc
    22861 cgagggcatg gacgtcaacc acatcaccat gcgaccgctc ggccaggcca actgacctcg
    22921 cgctcgcgcc ctgcccacga aagccgactc gaacaagcgg tcgagtgcac gcgtccgtac
    22981 gcgatggcgc gcggtgagtc gtacgcgatg tcgcgcggcg aggccgcacc ggtcatttga
    23041 cctgcccatg acgatgggca ggtcaaggca cttggtccgt cggcgatgtg ctcccggaca
    23101 aagaagagcc tcaccggtgt tttcgctggt gaggctcggt ttgcgccccc ggcaggactc
    23161 gaacctgcgg ccaagtgctt agaaggcacc tgctctatcc actgagctac gggggccggt
    23221 ggtggacgtg gatcacgggc cgcggacaag gataggggtc cgtgtgcctc gtcctggttg
    23281 cttcacctcc gtggcacgtt gtggaggttc ggtgaagcgg tcccgataat cgcaggcagg
    23341 tgcgaatcgc gcaccgcttt tggcgtctcg cgcggcgggt gttgtgtact cgttatgcct
    23401 gagtctctcc tgtcccatgt gccccgtgtg tcactgattc cgatttgtac gggttcagac
    23461 ggcgcacacg agggggtata cgcttcaaaa acccgccaaa attgggcatt ctgcacgtgt
    23521 ggcgacctcg gacgtacgac cccggatcat cgacgcgctg tccgcactgc gcgaccgcgt
    23581 cgatgccgca cgcttcccac tgccacttcc cggtgccgcc cgcgcccgac gcggccggtg
    23641 tgagctgctc gcccagctcg acgactatgt gatgccccgc ctgcgctccc ctcaggcgcc
    23701 cttgctcgcg gttgtcggcg gatccaccgg ggcgggcaag tccacgctcg tcaattcgct
    23761 ggtcggacgg cgggtgagtg aggcgggggt gctgcggccg acgacgcgta cgcccgtatt
    23821 ggtgtgccac cccgacgatc accattggtt cgccggacag cgggtgctgc cacagcttgc
    23881 caggatccgg acaccggggc aggaggacgc gggggccggt gcggcggccg gggcggaggc
    23941 gctgcggatc gagacggacg aggcgctgcc cagtgggctc gcgctgctgg acgcccccga
    24001 catcgactcg ctggtcgccc gcaaccggga gctggcggcc gagctgatct gtgccgccga
    24061 tgtgtgggtg ctggtcacca ccgccgcgcg ctacgcggac gcggtgccgt ggcacctgct
    24121 gcgtacggcg aaggagtacg acgtcacgct ggtcaccgtg ctcgatcgcg taccgcatca
    24181 gatcgccctg gacgtctcgg cacggtacgc ggcgctgctc gcacgggcgg gcctggccga
    24241 tgtcccccgg ttcaccatcc ccgagctgcc cgaatcggcg ggtggcggaa gcggactgct
    24301 gcccgcgacg gcggtggccg ggctgcgcga gtggctggaa cggcatgccc gggaccccat
    24361 cgcccgtacc gccgccgccc accgcaccag cgccgggatc atcgcctccc tgcgccgccg
    24421 tatgccggcg ctggccgggg cctgcgcggc gcagcacgcc gccgcgctgc gcctggccga
    24481 ccgggtcgag gaggcgtacg aggaggccgc ggagcgggtg cggaaggaga tcgccgaggg
    24541 ggaggtgctc tccggggacg cccgcgccca ctggagggac cacgcgctcg gtggacgccc
    24601 tgatgagctg ctggacgcgc tcacccgcgg gctcacctcc ctgctgcgct gcgctgtgga
    24661 gcaggcggac gagcggatcg ccgacgcgtg gcgccgcgac tcggtggctt tggccgctcg
    24721 gccggatacg gcggctgtgg gggtgctggg ggagggatgg gtgaacgggc cggaggtgag
    24781 gccggggatg aggttggggg tgaggccagg gatgaggtcg gggatgaggt ggcggacggg
    24841 gcggccgggc gggtgggggt tctcgtacgg cgttggcggc gctgccttga ggaactggcc
    24901 gaggacgagg tgcgggaggt gcgggaggaa cgtggggcgc gaggggcacg aggagcacga
    24961 ggggctatgg gggtacgagg ggtgggcacc ggggagcgtg cgggtgcgct cgacgcggag
    25021 gaagcggcgg cgctgctggc catcgcgctg ctcggcgggc ggcgggcgcg ggcggcgggg
    25081 cagcacctgg cgggactgct gggggcccat ggcgcgctgc ggctgcggga ccggggggaa
    25141 cggttgctca ggacgtacct ggagcacgcg ctggacggtg agcgggaacg acggctggct
    25201 ccgctggagt ggtggggagt gccgacggcc catcaggtcg ggctgatcgc cgcgctgtcc
    25261 gtattgcaga gggagcagcg cgagaagagg gagatggtgg cgcggtgagt gtttctgcgg
    25321 ggtcttggga ggcgaagggg aggggtgctt cgagtgaggt gggaggggag gagggtggcg
    25381 agtgtgggct ggtcgaggcc ggtgaggtgg gtgaagcgag tgaagggggt ggagtgggcg
    25441 aagtcagtga agtcggtgaa gtgggtgatg tgagaggtgt gagggaggag gttgaggggt
    25501 gggagggcgg gccggggggc tggatggggt gcgtggggtg tgggacgacg ggctgatcgc
    25561 gcgcagggcg ccggagggag gcccggcggg gtttgcggcc gagcggcgac gggcgcgggc
    25621 ggcggccgtg gcggaggcgc gtatgagagc accgggttgg gtggggctgg ccggaatggt
    25681 ggattcggag gggctggcgg atttgatggg ggtggccgac gggggcgata tgtggggcgg
    25741 ccgtgcgggc gaggacgccg gtacggactg gagcgcggac cggaacgcgg accggaacgt
    25801 gagcgggtac ggacgcgcgt cggccgggga gcggcgggcg gcggccacgg acgggctgcg
    25861 gaccaggctg gacgcgctgc gggaactggt cggcctgtcc cgtacgaggc tggacgggga
    25921 gacgctggcc gaggcggggc aggtgctgtt cgcggcggaa gagaggcgcc ggctgtccag
    25981 gaaccacacg gtggtcgcca tcgcgggagc gaccgggagc ggcaagtcgt cgctgttcaa
    26041 cgcgttggcc gggcaggaac ggtccgaggt gggggtacgt cgtccgacga cttcggagcc
    26101 cgtggcctgt gtgtgggcgg gtggccggtc aggggcggac gggctgctgc accggttggg
    26161 gattccggag gcgcggaggt gtgtgccggg gaacggctcg gggaccgggc ctgggaccgg
    26221 ttcgggggcc ggggccgggg gcgtggccgg tgcgatgggg ggaggcggga gtgggagcgg
    26281 gagcagggtc gggggtggat ccgggatctt cgggacagac ttcgcgtacg gctccgggag
    26341 cgccgccggg ctggacggac tcgtactgct ggatctgccc gaccacgact cggcggcagg
    26401 cgggcaccgc gagcaggtcg accggctgct gaaactggtg gacgcggtga tctgggtggt
    26461 cgacccggag aagtacgcgg acgccgtgct gcacgaaagg tatctgcggc ctttggcggg
    26521 ctacgcggag gtcaccttcg tggtgctcaa tcaggtggac cggctgcccg gcgaggccgc
    26581 cgaccaggtg gtggacgatc tgcgccggct gctggacgag gacgggctgg cgctggggag
    26641 cacggtgagc cgggtgcggt ggtgctcgcg ctgtcggcgg ccaccggcga aggggtcggt
    26701 gaactgcgcg agctgctcgg gcagttcgtc gcggagcgag gtgccgcggg ccggcggctg
    26761 accgctgatg tggacgcggt ggcggcgcgg ttgcggcccg tgtacgtggc ggaggggcgg
    26821 atcggcctga cggagggggc gcgtgaggac ttcgaggacc aactggccga ggcggtcggg
    26881 gcgatggcca tggggcgtgc ggcggaacgt gactggctgc ggcacgagga atgggcctgc
    26941 gggacggcgt gggggcggtt ccgtggacgt cgcgcggcgc ggccggcggc acggcgggtg
    27001 ggggcagggg gagcggcgcg gcggtctaag ggctggtcag gggcgcggtc ggaggatcgg
    27061 cgggggacgt ggtcggagga tcggctgcgg tcggatcaac tggcgcgccg gtctcggctc
    27121 gttcgcgtcg gggggctgtg gcgcctatgg ggagggcgga agctgaggcg agccggtgcg
    27181 ggtgcgggtg ccggagtgag tgtgggcgcg ggtgcgggcc cgggggcttg gggcggtggc
    27241 ggagtggggc ccggcgtgcg gccgtcggcc ggcagcggga tcttgggcgg cagtggaatc
    27301 ttgggcggca gtggtgctga tgctttggcc ggcagcggtg cgctgggcgg cagcggtgct
    27361 ctggaccggc gggcggcggc tcggccggtg gtggagcaag ccgtacgggc cgtggccggc
    27421 gaggcggctc gcggactgcc cgtgccctgg gcgcaggcgg tacgggaggc ggccgagcgg
    27481 ggggccgagg ggctgccgga ggcgctggac gccgtggtgg cggctgccga cggcgcgctg
    27541 cgggccgggc cgcccgtgaa gccgcggtgg tggacggtgg ccgccgccgc acagggactt
    27601 ctgctggccc tgcaagtggt cggcgtgatg tggctgctgg gagcggtgac gggagtgacg
    27661 ggggaggtgg cctggccggt ggcgttgttg ctcggcgcgg tggggacggc cggcgggccg
    27721 gcgctggcgt gggcgtgcgg gctgatggca cgtggaccgg cgcggcggta cgggcaggag
    27781 gccgaacggc ggctgcgcga cgcgtcggcg gcgtgcgggc gggcccgggt cctggaaccc
    27841 gtcgcggccg aactgttccg ctaccgggag gtgcgtgaac agtacgcggt ggcggcggg
//
