LOCUS       NZ_CP074380            13419 bp    DNA     linear   CON 27-MAY-2024
DEFINITION  Streptomyces sp. MST-110588 chromosome, complete genome.
ACCESSION   NZ_CP074380
VERSION     NZ_CP074380.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. MST-110588
  ORGANISM  Streptomyces sp. MST-110588
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7915503)
  AUTHORS   Booth,T.J., Bozhueyuek,K.A.J., Liston,J.D., Lacey,E. and
            Wilkinson,B.
  TITLE     Bifurcation drives the evolution of assembly-line biosynthesis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7915503)
  AUTHORS   Booth,T.J., Bozhueyuek,K.A.J., Liston,J.D., Lacey,E. and
            Wilkinson,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAY-2021) Molecular Microbiology, John Innes Centre,
            John Innes Centre, Norwich, Not US or Canada NR4, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 28-FEB-2016
            Assembly Method                   :: HGAP v. 3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 25.0x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022695595.1-RS_2024_05_27
            Annotation Date                   :: 05/27/2024 05:38:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,918
            CDSs (total)                      :: 6,828
            Genes (coding)                    :: 6,327
            CDSs (with protein)               :: 6,327
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 501
            CDSs (without protein)            :: 501
            Pseudo Genes (ambiguous residues) :: 0 of 501
            Pseudo Genes (frameshifted)       :: 350 of 501
            Pseudo Genes (incomplete)         :: 185 of 501
            Pseudo Genes (internal stop)      :: 14 of 501
            Pseudo Genes (multiple problems)  :: 45 of 501
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 12:56:23
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4914553
            Orig. end                         :: 4927972
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP074380.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Barrie Wilkinson, John Innes
            Centre, Norwich.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..13419
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="hydrogen-cyanide"
                     /region_number="21"
                     /rules="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD) and DAO)"
                     /tool="antismash"
     cand_cluster    1..13419
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /kind="single"
                     /product="hydrogen-cyanide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..13419
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:8419](+)"
                     /cutoff="5000"
                     /detection_rule="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /neighbourhood="5000"
                     /product="hydrogen-cyanide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..8419
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="5000"
                     /detection_rule="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /neighbourhood="5000"
                     /product="hydrogen-cyanide"
                     /protocluster_number="1"
     misc_feature    397..413
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 17.35"
                     /tool="antismash"
     misc_feature    402..415
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.5"
                     /tool="antismash"
     misc_feature    complement(405..421)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 17.12"
                     /tool="antismash"
     gene            458..895
                     /locus_tag="KGS77_RS21595"
                     /old_locus_tag="KGS77_21440"
     CDS             458..895
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019071757.1"
                     /locus_tag="KGS77_RS21595"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_21440"
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="WP_242584351.1"
                     /transl_table=11
                     /translation="MNQRQHKKLSRRLFVAILSGDTATVKAVLRAGIAADQPDGDGTTP
                     LYLASVNGEADIVRLLLKAGACPDTESGGGPGAEGTALCAAACWGHTETVRELLAYGAD
                     PNRREDHGTGRSPLDWANAGPHPETAGLLTAAGATVGGRTI"
     gene            complement(1006..1686)
                     /locus_tag="KGS77_RS21600"
                     /old_locus_tag="KGS77_21445"
     CDS             complement(1006..1686)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004000452.1"
                     /locus_tag="KGS77_RS21600"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_21445"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_242584353.1"
                     /transl_table=11
                     /translation="MNATQTPHGGCAKEKKKSGSGMPKTVDRDEQRRQIGDALLRLASE
                     RGLDEVSVRTVAAHSGRSPGAVQKYFRSKDEMLAFAAELAGERIEARMGRVDPGLPLRA
                     ALRDLVLATLPVDAERRAEAAAQLAFAGRAAHHAALAEVRRRIDREVREALAGWLASAG
                     YGHGPDPAADCLAVADAVIALSDGLALRMLYTPQDHEALLHALDRGLEALVPPHPPTDG
                     PSAP"
     misc_feature    complement(1760..1774)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.93"
                     /tool="antismash"
     gene            1826..2299
                     /locus_tag="KGS77_RS21605"
                     /old_locus_tag="KGS77_21450"
     CDS             1826..2299
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004000453.1"
                     /locus_tag="KGS77_RS21605"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_21450"
                     /product="DUF1707 domain-containing protein"
                     /protein_id="WP_242584355.1"
                     /transl_table=11
                     /translation="MDDRSGRSEQSDRREPAIRVSDQDREGALAVLSAALSEGRLDVKE
                     HAARSQLALEARTSEELTELTADLPAPPLTREERDRADLREWLAEWRYWLGGGVILSAI
                     WGVQCLKDHELRFYWPLAPLGIWAAVLVAIALWPRGKGDQEKGGQGKGGHGKK"
     gene            2472..>3026
                     /locus_tag="KGS77_RS21610"
                     /old_locus_tag="KGS77_21455"
                     /pseudo=""
     CDS             2472..>3026
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014142837.1"
                     /locus_tag="KGS77_RS21610"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_21455"
                     /product="hypothetical protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MGRGAMALARVAVVVRAPAGWLWWPGLLAAGTGALVPGLTGRRIG
                     LFAGAALFVLGAFVVFAVRRKRYMTLAGAGSRAGRSVILQDRAVTLRLWRRAHRWWLLV
                     AFLAAVASSFALPAAGGLLMAGCAVGLWAKALWLGRWERAHDALLWVRPESAVRGGAAG
                     KDVRGYLTTGVAAGDAGPGGGK"
     gene            complement(3243..4739)
                     /locus_tag="KGS77_RS21615"
                     /old_locus_tag="KGS77_21460"
     CDS             complement(3243..4739)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Orn_Arg_deC_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Orn_DAP_Arg_deC"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1264: diaminopimelate decarboxylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003950382.1"
                     /locus_tag="KGS77_RS21615"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_21460"
                     /product="diaminopimelate decarboxylase"
                     /protein_id="WP_242584357.1"
                     /sec_met_domain="Orn_Arg_deC_N (E-value: 4.2e-33, bitscore:
                     113.4, seeds: 15, tool: rule-based-clusters)"
                     /sec_met_domain="Orn_DAP_Arg_deC (E-value: 5.4e-09,
                     bitscore: 34.8, seeds: 500, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSATPDRPPSVPSDTSPDAFSDTCSASSDTSSTSGADRHRAVRAA
                     VERGLVGPDQPVAGLLDVTGIRRSAAELHAAFNRFMPPGTPVLHAFAVKAASLVPVLRL
                     LADEGLGCEVASPGELALARAAGVPASRTVLDSPAKTPAELREALDLGIAVNADNPEEL
                     ERIDAYIRRTGRTGGADGPGRTGRTGGTSGTGDPAPTTSPLGLRVNPQIGGGRIGAMST
                     ATTTSKFGFALRDPGARDQVVRACLDRPWLTRLHAHVGSQGMAPALMAEGVQALFDLAE
                     EVNEKAGRAQITTLDIGGGLPVNFASDTTAPDYDAYAALLHSAVPGLFSGRYGLVTEFG
                     RSLLAKHGTVLARVEYAKSAGGRPIAVTHAGAQVATRTVFAPAAWPIRVAAYDGRGRPK
                     TGAPVPQDVAGPCCFAGDLVAEDRPLPLLASGDHVALLDTGAYYFSTHFAYNSLPRPGV
                     YGYTTAEDGTVRFATVRSPQTLEELTTESGAAHRDSLSGL"
     gene            5001..6428
                     /locus_tag="KGS77_RS21620"
                     /old_locus_tag="KGS77_21465"
     CDS             5001..6428
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /GO_function="GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1103: FAD dependent oxidoreductase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018514374.1"
                     /locus_tag="KGS77_RS21620"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_21465"
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="WP_242584359.1"
                     /sec_met_domain="DAO (E-value: 2.2e-61, bitscore: 207.1,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRVTRVQTGQPVVGHPDVIVVGSGIIGAACAESLTRRGIRVTVLD
                     RGPLASGTTASGEGNLLVSDKPPGPELALTQASLERWPRLLAELGGGGAYGDARDGAYG
                     AAFDGAHGGAYGGARGGAYGCEYEAKGGLVVALEDAGAQRLRAFAAAQRGAGVQVHDIS
                     PDEVSTYEPHLTPGVRAAAYYPQDAQLQPVLAATTLLAAVRARGAEVRTGVTALGVEQA
                     ADGRVTAVRTCAGPLPCGAVVNACGPWAGRFAEAAGAPLPVLPRRGMVLVTTALPPGTV
                     RHKVYDAGYVGAVGSGEADLRTSAVVESTHGGTVLIGSSRQRVGFDATVRVAVLRELAR
                     NAMALFPALGRVPVIRAYGGFRPYTPDHLPVIGEDPRRPGLWHATGHEGAGIGLAAATG
                     ELLAGLYAGEPPLVDPAPFRVDRPGLGLGADPERIRTSGSGSGPGSDSGSGPGSGAGPG
                     PGSGSGAEGSEGLSCSV"
     misc_feature    5433..5435
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            6416..6691
                     /locus_tag="KGS77_RS21625"
                     /old_locus_tag="KGS77_21470"
     CDS             6416..6691
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Fer2_4"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018839558.1"
                     /locus_tag="KGS77_RS21625"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_21470"
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="WP_242584361.1"
                     /sec_met_domain="Fer2_4 (E-value: 1.6e-23, bitscore: 81.2,
                     seeds: 114, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFRVTVDGERRPARPGQTVAAVLLAAGRVSWRTTRGGRPRGVFCG
                     IGVCHDCLVVVNGLPDVRACRREVEDGDDIRTQNGAELPSAEADRG"
     gene            6684..7289
                     /locus_tag="KGS77_RS21630"
                     /old_locus_tag="KGS77_21475"
     CDS             6684..7289
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pyr_redox_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /locus_tag="KGS77_RS21630"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_21475"
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="WP_277994249.1"
                     /sec_met_domain="Pyr_redox_2 (E-value: 1.4e-07, bitscore:
                     29.7, seeds: 64, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MADGEGTAAGPGAGAEAGSGAGRNTGRKAGRNAGPNAGRQAGPVV
                     VIGAGPAGTQAALAAADAGVRVILVDDGPRTGGQYHRRAPDAPVPAWEREIDAHPRIER
                     LSRTAVWALEPLPHAPHAPDDPCAPHVPDAPHAPQDPATPAPREPKALPPRGPSTSSRA
                     PPTPPAGRPVPSVRPPWSCAPARTTGHCRSPAGTCRAW"
     gene            7253..8419
                     /locus_tag="KGS77_RS21635"
                     /old_locus_tag="KGS77_21480"
     CDS             7253..8419
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Fer2_BFD"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Pyr_redox_2"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /locus_tag="KGS77_RS21635"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_21480"
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="WP_242587607.1"
                     /sec_met_domain="Pyr_redox_2 (E-value: 6.3e-09, bitscore:
                     34.1, seeds: 64, tool: rule-based-clusters)"
                     /sec_met_domain="Fer2_BFD (E-value: 4.3e-10, bitscore:
                     38.5, seeds: 211, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPFPGWDLPGVVTAGAAQALAKGQRTAIGRRVVLSGTGPFLLPVA
                     SSLLAAGARVLAVYEAGSPAAWLRSPLAALRDGRGKLPELAGYAAELARHRVPYRPRHA
                     VVAAHGGERVESVSVARLTPNWRIRPGSERRVTGVDAVCVGYGFTPQLELALAAGCALR
                     ANAWVAVDAWQATSVPGVYAAGEPTGIGGAELAAAEGEVAGIAAAAHLGARPDLPRADA
                     ARRRVLAGRRFARLLAAAHPVRPGWRSWLRPDTLICRCEEVTYGELRAGAREHGADGMR
                     ALKLTTRVGLGPCQGRICARNAAELLTGLPAGPATGLPAGLATGLPGLPAGLPDPHTAD
                     RRPLAQPVRLADLAAVPAEPLPDHSEPQSDRITQEPDHPASERTPYDE"
     gene            8409..9440
                     /locus_tag="KGS77_RS21640"
                     /old_locus_tag="KGS77_21485"
     CDS             8409..9440
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /GO_process="GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /GO_process="GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019358495.1"
                     /locus_tag="KGS77_RS21640"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_21485"
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="WP_242584363.1"
                     /transl_table=11
                     /translation="MTNDVTPPTEPPTKSSTKPSAKPSPQAQAPAESPARASSAALTGV
                     IVATTLPYAEDATAPAGLRPDLDRYAEHCRRLVDNGCHGVGPNGSLGEYSSLTDEERRA
                     VARTAVAAVGRDATVVVGVHGVGAHQARHWAELAAEDGADGVLCLPPTLYRANTSEVVE
                     HFTQVAAVGLPVMVYNNPLDTRVDLTPEMLREIAAIDNVVAVKEFSGDIRRILHIKEHA
                     PGLEVVAGADDLVLEALLMGATGWFAGFPNVFPAECVRLYDLARAGRLAEARALYEPLV
                     AAFRWDSRTEFVQAIKHSMDLVGCYGGPCRPPRGPLDAALLKQLDADMQRAVQAVQALE
                     AAD"
     gene            9440..10534
                     /locus_tag="KGS77_RS21645"
                     /old_locus_tag="KGS77_21490"
     CDS             9440..10534
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016576245.1"
                     /locus_tag="KGS77_RS21645"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_21490"
                     /product="proline racemase family protein"
                     /protein_id="WP_277994250.1"
                     /transl_table=11
                     /translation="MAAERSRPTHTSRPPRAPRWAAGARRFSVVDSHTEGMPTRVVTDG
                     FGVIPGATMAARRRHFVEHLDHLRRLLMNEPHGHPAMSGAILQPPTRPDADWGVVYIEV
                     SGCLPMCGHGTIGVATVLVETGLVTVTEPVTTVSLDTPAGLVRAEVAVRDGRAEHVTIH
                     NVASYAHELDATVEVPALERDLGLDTAEIRYDMAYGGNFYAITPLAALGIPFTTARKDD
                     ILAAGLAVMDAINKHNRPVHPTDPTISGCRHVQFTAPGTPGPGGSDARNAMAIHPGWFD
                     RSPCGTGTSARMAQLHARGELPLHQDFVNESFIGTRFTGRLVGTDRVGDRPAVTPTVTG
                     RAWITGMGDHYLDPTDPFPEGFVL"
     gene            complement(10687..11697)
                     /locus_tag="KGS77_RS21650"
                     /old_locus_tag="KGS77_21495"
     CDS             complement(10687..11697)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1016: LuxR family
                     DNA-binding response regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003955806.1"
                     /locus_tag="KGS77_RS21650"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_21495"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_242584365.1"
                     /transl_table=11
                     /translation="MLVALGLKSIHESVYQAMLKHDTWGVAELAAHLAFTEDEVRAALD
                     ALFELRLIRESFDAPGRLRAVDAGTALRALLDRQHEELLRRQRQAADRHAAIARMIAQA
                     AAHAWDHGAALPAGGAAPGGTEQLLGMDAVQQRLEQLAEEAAEEILTFMPGGAQSAAAL
                     DAARRNDARVLARGVSMRTVGLDSLRQDPPTLAHARWLTGRGAAFRTVAALPPRMIVAD
                     RRAALVPLDPADTRKGVLYVTCPGVIASLTALFLQVWEHATSLGADAPAPRPGLSDTER
                     DLLHLIGQGYTDEAAATRLHISPRTARRMMAAIMERLGARSRFEAGLKAAREGWL"
     misc_feature    complement(11509..11511)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            12121..12396
                     /locus_tag="KGS77_RS21655"
                     /old_locus_tag="KGS77_21500"
     CDS             12121..12396
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="KGS77_RS21655"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="KGS77_21500"
                     /product="hypothetical protein"
                     /protein_id="WP_242584367.1"
                     /transl_table=11
                     /translation="MRLTLITLTGTGHPPPHGLQAPLLVDLLWAAATPADRVEHVSARA
                     GPGHIDLGVYTRAPYGESARAAVAVVRRALALNSSLRGWALTRVRE"
     misc_feature    complement(12421..12436)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.11"
                     /tool="antismash"
     gene            12432..12749
                     /locus_tag="KGS77_RS21660"
                     /old_locus_tag="KGS77_21505"
     CDS             12432..12749
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014152270.1"
                     /locus_tag="KGS77_RS21660"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_21505"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_242584379.1"
                     /transl_table=11
                     /translation="MTENRDGLDDDMDNGVDSRMGGDSFQDVLSMLSEREREVLAHLAA
                     GCTYGTIARRMGLSSHTVDTYIRRIRGKTGVANRVELTLLALAAGSHGKGGKVPSGTAK
                     P"
ORIGIN
        1 cgctggtcga ggacctgccg gccggagccc ggagcttcgc cactgcccca ggccactacg
       61 acttctccag caagcgatgt gtgaaggacc tggaactccg gcacgtccgg ggtatcggat
      121 ccgatgaccg gcagatggag atccacttcc ggcacaactg ctggaagcac gacgaagacc
      181 tggtcatccg ctacacgggc gtcttcggct gccatgccgc gagcgccggg ggcgaggact
      241 ggccacatct ggggaccgtg atcctcgacg aggtcctgcc ccaccaggac ggctgcagcc
      301 acgagatcgc gttctggaac ggctcgctca ccgtcgtctg ccgtgacctc acagcgacct
      361 ggacccaggc tgactgcccc ggcaatccat gaaatccagg aaatctataa caatatgact
      421 ggcttcgcca cgtatccggt cgttaacctc ggccctcatg aaccagcggc agcacaagaa
      481 gctctcccgg cgcctgttcg tcgcgatcct gtcgggtgac accgccaccg tgaaggcggt
      541 actgcgggcc gggatcgcgg ccgaccagcc ggacggtgac ggtacgaccc ctctgtacct
      601 ggcgtccgtc aacggtgaag cggacatcgt ccgcctgctc ctcaaggccg gcgcctgccc
      661 cgacaccgag agcggcggcg gacccggcgc ggagggcacg gcgctgtgcg cggctgcgtg
      721 ctggggacac accgaaacgg tgcgtgaact gctggcgtac ggtgccgatc ccaaccggcg
      781 cgaggatcac ggaaccggcc gctccccgct ggactgggcc aacgcgggcc cccaccccga
      841 aaccgccgga cttctcaccg cggcgggagc caccgtcggc gggcggacca tataggacca
      901 cgtagcgggg gcgtcccggt ccggcacctt cggcatgtgg catttctttt cgcccggccg
      961 gtcacagggg cgaattcatc gttctcgtat gtggtgtcca gccccctatg gggcgctcgg
     1021 tccgtcggtg ggcgggtgtg gggggaccag cgcttccagg ccgcggtcca gggcgtgcag
     1081 cagggcctcg tggtcctgtg gggtgtacag catgcgcagt gccagaccgt ccgagagtgc
     1141 gatcacggcg tcggcgacgg ccaggcagtc cgcggccggg tcggggccgt gcccgtaccc
     1201 ggcggaggcc agccagcccg cgagtgcctc gcgtacctcc cggtcgatcc ggcggcggac
     1261 ctcggcgagt gcggcgtggt gggcggcgcg gccggcgaag gcgagctggg ccgctgcctc
     1321 ggcccggcgt tccgcgtcga ccgggagggt ggccagcacc agatcccgta gcgcggcgcg
     1381 gagtgggagg ccggggtcga cgcggcccat gcgtgcctcg atgcgctcgc ccgccagctc
     1441 ggcggcgaag gcgagcatct cgtccttgga gcggaagtac ttctgcaccg ctccggggga
     1501 gcggccactg tgcgcggcca ccgtacggac gctgacctcg tccaggccgc gttcggacgc
     1561 caggcgcagc agggcgtcac cgatctgccg gcgctgttcg tcccggtcca ccgttttggg
     1621 catgccgctc ccgctcttct ttttctcttt cgcgcagccg ccgtgtggcg tctgagtggc
     1681 gttcatgtgg cgtccgtcca gcttgccccg gcttccgccg aagatcgccc ggcccgccca
     1741 cggctcttta ggatacagtc gtattctgaa gatacggccc ggggtgtacc gcccggggat
     1801 ccagtaccga agggggactc catccatgga cgaccggagt ggccgcagtg agcaaagtga
     1861 ccggcgcgaa cctgccatcc gcgtctcgga ccaggaccgc gagggcgcgc tcgcggtgct
     1921 ctccgccgcg ctctccgagg gccggctcga cgtcaaggaa cacgcggccc gctcgcaact
     1981 ggccctggag gcccgtacga gcgaggaact caccgaactg accgccgacc tgcccgcccc
     2041 gccgctgacc cgcgaggagc gcgaccgcgc ggacctgcgg gaatggctgg ccgagtggcg
     2101 ttactggctc ggcggcggcg tgatcctttc ggcgatctgg ggtgtgcagt gtctgaagga
     2161 ccatgaactg cgcttctact ggccgctggc gccgctgggg atctgggcgg ccgtactggt
     2221 ggcgatcgcg ctctggccgc gggggaaggg tgatcaggag aagggcggtc aggggaaggg
     2281 cggtcacggg aagaagtgag ttctcgcctc gtgcgtagtc cttgcctgct gcttgcgcgg
     2341 tcgtagggga ctcttcgctc accgggggac tcgtcgcgta ccggggactc gtcgcgtact
     2401 caggaaatcc tcagacgtac tggctaccat ccgccgccgt aggaacggat ccgggcagga
     2461 ggaggtcggt cgtggggcgc ggagcgatgg cgctggcgcg ggtggcggtg gtggtacggg
     2521 cccccgccgg atggctgtgg tggccggggc tgctggccgc ggggacgggt gcactggtgc
     2581 cggggctgac cggccggcgg atcgggttgt tcgccggagc ggcgcttttc gtgctcggtg
     2641 cgttcgtggt cttcgcggta cggcgcaagc ggtacatgac gctggcgggc gcggggtcgc
     2701 gggccggcag gtcggtgatc ctccaggacc gggcggtcac gctgcggctc tggcgacggg
     2761 cccaccgctg gtggctgctg gtcgcgttcc tggccgcggt cgcctcatcc ttcgcgctgc
     2821 ccgccgccgg cggcctgctg atggccggct gcgcggtcgg cctgtgggcc aaggcgctgt
     2881 ggctggggcg ctgggagcgg gcgcacgacg cgctcttgtg ggtacgtccg gagtcggccg
     2941 tacggggcgg cgcggcgggc aaggacgtgc gcggatatct gacgaccggt gtggcggcgg
     3001 gggacgcggg gccgggcggc gggaaggcgc cggtccggcg agaggccggc aaggcggcgg
     3061 ggcgggcagg ccggcaggcc ggtgggcagg ctggtcggca ggccggtggg ccggtgggcg
     3121 gtcgaccgcc ggggccgctg acgccgtgcg gacggccggg tagccgggcc gctgacctgc
     3181 cgggccggcg gcccgctgga ccggaagcgg tgccgagcgg tgcccagcgc agccgagcgg
     3241 agtcagagcc cggagaggct gtcgcggtgg gcggcgccgc tttcggtggt cagctcctcc
     3301 agggtctgcg gggagcgtac ggtcgcgaag cgcacggtgc cgtcctccgc cgtcgtatag
     3361 ccgtaaacgc cgggccgggg cagggagttg taggcgaagt gggtggagaa gtagtaggcg
     3421 ccggtgtcca gcagggcgac gtggtcgcct gacgccagga gcggcagcgg gcggtcctcg
     3481 gcgaccaggt cgccggcgaa gcagcagggc ccggcgacgt cctgcggcac cggcgcgccg
     3541 gtcttggggc ggccccgccc gtcgtacgcc gcgacccgga tcggccaggc ggccggggcg
     3601 aagaccgtac gggtggcgac ctgggcgccc gcgtgggtca cggcgatcgg ccggccgccg
     3661 gcggacttgg cgtactcgac gcgggccagg accgtgccgt gcttggcgag cagggacctg
     3721 ccgaactcgg tgaccaggcc gtagcggccg gagaagagcc cggggacggc ggagtgcagc
     3781 agggccgcgt acgcgtcgta gtcgggcgcc gtggtgtcgg aggcgaagtt cacggggagg
     3841 ccgccgccga tgtccagggt ggtgatctgt gcccggccgg ccttctcgtt gacctcctcg
     3901 gcgaggtcga acagcgcctg tacgccttcg gccatgagcg cgggagccat gccctgggag
     3961 ccgacgtggg cgtgcagccg ggtcagccac ggacggtcca ggcaggcccg tacgacctgg
     4021 tcgcgggcgc ccgggtcgcg cagcgcgaag ccgaacttgg aggtggtggt ggcggtgctc
     4081 atcgcaccga tgcgaccgcc gccgatctgc gggttgacgc ggaggccgag gggggaggtg
     4141 gtgggtgcgg ggtcgccggt gccgctggtg ccgccggttc tgccggttct gccggggccg
     4201 tcggctccgc cggtacgtcc cgtccgccgg atgtacgcgt cgattctctc cagttcctcc
     4261 ggattgtcgg cgttgacggc gatgcccagg tccagtgcct cgcgcagttc ggccggggtc
     4321 ttggccgggg agtccaggac ggtccgggac gcggggaccc cggcggcgcg ggccagcgcc
     4381 agttcgccgg ggctggcgac ctcgcagccc agcccttcgt cggcgagcag ccgcaggacg
     4441 gggacgagcg aggcggcctt gacggcgaag gcgtgcagga ccggggtgcc cggcggcatg
     4501 aaccggttga aggcggcgtg gagttcggcg gcggaccggc ggattcctgt gacgtccagg
     4561 agaccggcca ccggctggtc ggggccgacg agcccgcgct cgacggcggc ccgtacggca
     4621 cggtggcggt ccgctccgga ggtggaggag gtgtcggagg aggcggagca ggtgtccgag
     4681 aaagcgtcgg gggaggtgtc ggaggggaca gaggggggcc ggtcgggcgt ggcgctcatg
     4741 ggaccacttc accattcacc gggtccggct gccagggaaa ccgcggaccc cggctgccgc
     4801 cgctcggacg gggccgagcc tcttacggtt tgcggcgcat ccggcccccg acaccgatcg
     4861 ttcgctaccg attttccgct actgatcgtt cgctgtccgt cgccgatcag ttgtgccgag
     4921 gtgttgacca cgcggtggcg gcggcaatag cgtcacggcg ctccggtgca aagatccctc
     4981 gcggaacggc ggtgcggtgc atgcgagtca cgcgcgtcca gaccggtcaa cctgtggtcg
     5041 gccaccccga cgtgatcgtc gtcggaagcg ggatcatcgg cgccgcctgc gccgaatccc
     5101 tcacccggcg cggcatacgg gtcaccgtcc tggaccgcgg gccgctcgcc tccggcacga
     5161 ccgcgagcgg cgaaggcaat ctcctggtct ccgacaagcc gccggggccc gaactcgcgc
     5221 tcacacaggc ttcgttggag cgctggccgc ggcttctggc ggaactgggc ggcggcggtg
     5281 cgtacggcga tgcgcgcgac ggtgcgtacg gcgctgcttt cgatggtgcg cacggtggcg
     5341 cgtacggcgg tgctcgcggt ggtgcgtacg gctgcgagta cgaggccaag ggcggcctcg
     5401 tcgtcgccct cgaagacgcg ggcgcccagc ggttacgcgc cttcgccgcg gcccagcgcg
     5461 gcgccggcgt ccaggtccac gacatctcgc cggacgaggt gtccacgtac gaaccgcatc
     5521 tgacgcccgg cgtccgcgcc gccgcctact acccccaaga cgcccagctc cagccggtgc
     5581 tggccgccac caccctgctc gccgccgtcc gtgcccgcgg tgccgaggtc cgtacgggcg
     5641 tcaccgcgct cggtgtggag caggcggcgg acggacgggt cacggccgta cggacctgtg
     5701 ccggtccgct gccgtgcggc gcggtcgtca acgcctgcgg accctgggcg ggccggttcg
     5761 ccgaggcggc cggcgccccg ctgcccgtac tgccgcgccg gggcatggtc ctggtcacca
     5821 ccgcgctgcc gccgggcacg gtccggcaca aggtgtacga cgccgggtac gtgggcgcgg
     5881 tcggcagcgg cgaggcggac ctgcggacct cggcggtggt cgagtcgacg cacggcggaa
     5941 ccgtgctgat cggctccagc cggcagcgcg tcggcttcga cgcgaccgtg cgggtcgcgg
     6001 tcctgcggga gctggcgcgc aacgcgatgg cgctgttccc ggcgctggga cgggtgccgg
     6061 tgatacgggc ctacggcggc ttccgcccgt acacccccga ccatctgccg gtcatcggtg
     6121 aggacccgcg gcggcccggt ctgtggcacg cgaccgggca cgagggggcg gggatcggcc
     6181 tggcggcggc caccggcgaa ctcctggccg ggctgtacgc gggcgagccg ccgctcgtgg
     6241 accccgcgcc gttccgcgtg gaccggccgg ggctgggact gggggcggac cctgagcgga
     6301 tccggacgtc ggggtcgggg tcagggccgg ggtcggattc agggtcgggg ccaggttcag
     6361 gggcgggccc gggtccgggg tcagggtcgg gggcggaagg atcggagggg ctgtcgtgtt
     6421 ccgtgtgacg gtggacggtg agcggcggcc ggcccggccg ggccagacgg tggccgcggt
     6481 cctgctggcc gcgggccggg tgtcctggcg tacgacccgg ggcgggcgcc cgcgcggcgt
     6541 cttctgcggg atcggcgtct gccacgactg cctggtcgtc gtcaacgggc tgccggacgt
     6601 acgggcctgc cgccgcgagg tcgaggacgg cgatgacatc cgtacgcaga acggggcgga
     6661 actgccctcg gcggaggcgg accgtggctg acggggaggg aacggcggcc ggaccgggtg
     6721 ccggggcgga ggcgggatcg ggcgccgggc ggaacaccgg gcggaaggcc gggcggaacg
     6781 ccgggccgaa cgccggacgg caggccgggc cggtagtggt gatcggggcc gggcccgccg
     6841 gcacccaggc cgcgctcgcc gccgccgacg cgggcgtccg cgtcatcctt gtggacgacg
     6901 gcccccggac cggcggccag taccaccgac gcgccccgga cgcgccggta cccgcctggg
     6961 agcgggagat cgacgcccat ccgcgcatcg aacggctctc ccgtacggcg gtctgggccc
     7021 tggaacccct gccacacgcc ccgcacgctc cggatgaccc ctgcgccccg cacgtcccgg
     7081 acgccccaca cgccccgcag gacccggcca cccccgcgcc ccgcgagccg aaggcgctcc
     7141 ctccgcgtgg gccgtccacc tccagcaggg ccccgccgac gccgccggcg ggccgccccg
     7201 taccgtccgt gcgtccgccc tggtcctgtg caccggcgcg tacgaccggg cactgccgtt
     7261 ccccggctgg gacctgccgg gcgtggtgac ggccggcgcg gcccaggcgc tcgccaaggg
     7321 gcagcggacg gcgatcggcc gccgcgtggt cctctccggc accggcccgt tcctgctgcc
     7381 cgtcgcctcc tcgctgctgg cggccggtgc ccgggtactg gccgtgtacg aggcgggctc
     7441 ccccgccgcc tggctgcgct ccccgctcgc cgctctccgg gacggccgtg gaaaactccc
     7501 cgaactcgcc gggtacgcgg ccgaactggc ccgccaccgc gttccctacc gcccccgcca
     7561 cgcggtcgtc gcggctcacg gaggggagcg cgtcgagtcg gtgagcgtcg cccgtctcac
     7621 ccccaactgg cggatacggc ccggcagtga gcggcgggtg acgggcgtgg acgcggtgtg
     7681 cgtcgggtac gggttcacgc cgcagctcga actcgccctc gccgcggggt gcgcgctgcg
     7741 tgcgaacgca tgggtggccg tcgacgcctg gcaggccacc agcgtccccg gcgtgtacgc
     7801 ggcgggcgag ccgaccggca tcggcggcgc ggaactggcc gccgccgagg gcgaggtggc
     7861 cggtatcgcg gcggcggccc acctcggcgc gcggccggac ctgccccgcg cggatgccgc
     7921 ccgccgccgc gtcctggcgg gccgccgctt cgcccggctg ctggccgccg cccatcccgt
     7981 acggccgggc tggcggtcct ggctccggcc ggacaccttg atctgccgct gcgaggaagt
     8041 gacgtacggg gaactgcgcg ccggtgcccg ggaacacggt gcggacggta tgcgtgccct
     8101 caagctgacc actcgtgtgg ggctgggccc gtgccagggc cggatctgtg cccgtaacgc
     8161 ggccgagctc ctgaccggcc ttcctgccgg ccccgcgacc ggtctcccgg ccggccttgc
     8221 taccggtctc cccggtctcc cggcgggtct ccccgatccg cacaccgccg accgccgtcc
     8281 gctggcccag ccggtccgcc tcgcggacct cgccgcggta ccggccgagc ccctgccgga
     8341 tcactcggaa ccgcaatccg accgcatcac tcaggaaccc gatcaccccg cttccgagag
     8401 gacgccatat gacgaatgat gtgacgcccc cgaccgagcc cccgaccaag tcctcgacca
     8461 agccgtcggc caagccctcg ccccaggccc aggcaccggc cgagtccccg gcccgcgcct
     8521 cctccgccgc gctcaccggt gtcatcgtcg ccaccaccct cccgtacgcc gaggacgcca
     8581 ccgcgcccgc cgggctgcgg cccgacctgg accggtacgc cgagcactgc cgccggctcg
     8641 tcgacaacgg gtgtcacggt gtgggcccca acggctcact gggggagtac tcctcgctca
     8701 ccgacgagga gcggcgcgcg gtcgcccgta cggccgtggc ggccgtcggc cgggacgcga
     8761 cggtggtcgt cggcgtgcac ggcgtcggcg cccaccaggc ccggcactgg gcggaactgg
     8821 ccgccgagga cggcgccgac ggcgtactgt gcctgccgcc gaccctctac cgcgccaaca
     8881 cctccgaggt ggtggagcac ttcacgcagg tcgcggccgt cggcctgccc gtcatggtct
     8941 acaacaaccc tctggacacc agggtcgacc tcacccccga gatgctgcgc gagatcgccg
     9001 cgatcgacaa cgtcgtcgcg gtcaaggagt tctccggcga catccggcgc atcctccaca
     9061 tcaaggagca cgcgcccggg ctggaggtcg tggccggcgc cgacgacctg gtgctggagg
     9121 cgctgctgat gggggccacc ggctggttcg ccggcttccc caacgtcttc cccgccgagt
     9181 gcgtacgcct gtacgacctc gcccgcgccg gccgtctggc cgaggccagg gccctgtacg
     9241 agccgctggt cgccgccttc cgctgggact cgcgcaccga gttcgtccag gcgatcaagc
     9301 actccatgga cctcgtcggc tgctacggag gcccctgccg gccgccgcgc ggaccgctgg
     9361 acgcggccct cctcaagcag ttggacgccg acatgcagcg cgccgtccag gccgtccagg
     9421 ccctggaagc ggccgactga tggcggcgga gcggtcccgg ccgacccaca cctcccgccc
     9481 gccccgggcc ccacggtggg cggccggggc ccgccgcttc agcgtggtcg actcgcacac
     9541 cgagggcatg cccacgcggg tcgtcacgga cggcttcggg gtgatccccg gcgccaccat
     9601 ggccgcccgc cgccggcact tcgtcgaaca cctcgaccac ctgcgccggc tgctgatgaa
     9661 cgagccgcac gggcacccgg cgatgagcgg cgcgatcctc cagccgccca cccgcccgga
     9721 cgccgactgg ggcgtggtct acatcgaggt ctccggctgc ctgcccatgt gcgggcacgg
     9781 gacgatcggg gtggcgaccg tcctggtgga gaccgggctg gtgacggtca ccgagcccgt
     9841 caccaccgtc agcctggaca ccccggcggg gctggtccgg gccgaggtcg ccgtccgcga
     9901 cggccgcgcc gaacacgtca ccatccacaa cgtcgcctcc tacgcccacg aactcgacgc
     9961 gaccgtcgag gtgccggccc tggaacggga cctcggcctg gacaccgccg agatccgcta
    10021 cgacatggcc tacggcggca acttctacgc catcaccccg ctcgccgccc tgggcatccc
    10081 cttcaccacc gcccgcaagg acgacatcct ggccgccggg ctggccgtca tggacgccat
    10141 caacaaacac aaccgtcccg tccaccccac cgatcccacg atctccggct gccgccacgt
    10201 ccagttcacc gccccgggca cccccgggcc gggcggcagc gacgcccgca acgccatggc
    10261 catccacccc ggctggttcg accgctcacc atgcggcacg ggcaccagcg cacgcatggc
    10321 ccagctccat gcccgtgggg aactccccct ccaccaggac ttcgtgaacg agtccttcat
    10381 cggcacccgc ttcaccgggc gcctggtcgg caccgaccgg gtcggcgacc gtccggccgt
    10441 cacgcccacg gtcaccggcc gcgcctggat caccggcatg ggcgaccact acctggaccc
    10501 gaccgacccc ttccccgagg gcttcgtcct ctgattcgtc tgattcgtcc gctgtttcgt
    10561 ccgctgattc cgatgacgtc acgccggtga ccgccggccg gggcacggcg cgcggagcca
    10621 ggcggcccgg cgcacggcgt acggcgtacg gcacacagac ccaggcgtac gacgctcacg
    10681 ccggtctcaa agccaccctt cccgggcggc cttgaggccg gcttcgaagc ggctgcgggc
    10741 gcccaggcgc tccatgatgg ccgccatcat gcggcgggcc gtacggggcg agatgtgcag
    10801 cctggtcgcg gcggcctcgt ccgtgtatcc ctggccgatc aggtggagca ggtcgcgttc
    10861 ggtgtcgctc aggcccgggc ggggtgcggg cgcgtcggcg cccagtgagg tggcgtgttc
    10921 ccagacctgg aggaacagcg cggtcaggga cgcgatgacg ccggggcagg tcacgtacag
    10981 gacgcccttg cgcgtgtcgg cggggtccag ggggacgagc gcggcgcggc ggtcggcgac
    11041 gatcatgcgc gggggcagcg cggccaccgt acggaatgcg gcgccgcggc cggtgagcca
    11101 gcgtgcatgg gccagggtcg gcgggtcctg ccggaggctg tccaggccga cggtgcgcat
    11161 gctgacgccc cgggcgagca cccgggcgtc gttgcggcgg gcggcgtcca gcgcggcggc
    11221 ggactgggcg ccgccgggca tgaaggtgag gatctcctcg gcggcctcct cggcgagctg
    11281 ctccagccgc tgctgtacgg cgtccatgcc caggagctgt tcggtgccgc ccggggcagc
    11341 gccgccggcg gggagggcgg cgccgtggtc ccaggcgtgc gcggcggcct gggcgatcat
    11401 gcgggcgatg gccgcgtgcc ggtccgccgc ctgccgctgc cggcgcagga gttcctcatg
    11461 ctgccggtcc agcagggcgc gcagtgcggt gccggcgtcc acggcgcgta accggccggg
    11521 tgcatcgaac gactcgcgga tcagccgcag ttcgaacagg gcgtccaggg cggctcgtac
    11581 ctcgtcctcg gtgaaggcga gatgggcggc cagctcggcg acgccccagg tgtcgtgctt
    11641 cagcatcgcc tggtacaccg actcgtgtat cgacttcagt cccaacgcta cgagcacgcg
    11701 tccactcctg ccgtctgcgg tgacgtacgt ttctgctggg gcgggggagt gctactgccc
    11761 caaggtgcgc gcattgtcca ccggtgcgca tcaacaacgg atcaacggcc gtacgggtgg
    11821 gtgcgtacgg gtacgggagc gggccgcgcc ctcggggccg ttgaggctgg tggtgtgcct
    11881 gtggctcccg gcaagaggtg cggcgaggtc tggcacaaaa cggcctggca ggttccgccg
    11941 tggccaagac ccttttccgc ccgtgggcat cgggcgaaag tactgctcac cgggcggccg
    12001 gtacgtcccg tacgtacccg ccacagacct cgcacatcag acaggggtgt tcccatggct
    12061 cttttgattc tgcgcgccac cggcagcctg atctgggact gagcgcgcac ggcaaccggt
    12121 atgcgcctga ccctgatcac cctgaccgga accggtcatc cgccgccgca cgggctccag
    12181 gccccgctgc tggtggacct gttgtgggcc gcggcgaccc cggccgaccg ggtcgagcac
    12241 gtcagcgcgc gggccggccc cggacacatc gacctgggcg tctacacccg tgccccgtac
    12301 ggggaatccg cccgcgccgc cgtggcggtc gtccgccggg ccttggcgct gaattcctcc
    12361 ctccgcggct gggccctgac ccgggtccgg gaatagcggt cacgctttca ccagaaaggc
    12421 gggacgacgt cgtgacggaa aacagagacg gtttggatga cgacatggac aacggtgtgg
    12481 acagtcgcat gggcggcgac agttttcagg acgtgctttc catgctctcc gagcgcgagc
    12541 gcgaggtgct ggcccatctc gcggcgggct gcacgtacgg cacgatcgcc cgccgcatgg
    12601 gcctgagttc gcacaccgtg gacacctata tccggcgtat ccgcggcaag accggggtgg
    12661 ccaaccgcgt agagctcacg ctgctcgccc ttgccgcggg cagccacgga aagggcggga
    12721 aggttccctc cggtaccgcg aaaccgtgac tgtcggccgg ttcgccggcc cggcagagta
    12781 atccccgtaa gcagcggccg ggccacaaag gccggaccgc ggcggacaaa ggaaaaccgc
    12841 ggacgtctca accggagact cccggaggac acggaaagag ggattgacat ggcgaaggcc
    12901 atcactcgcg cgctcggcat gaagtcgaag gtcggcggcc gttacatcca cttcgacgcc
    12961 ttcccgaagg cgaccatggg cggccgttac atccacgtcg gctgaacagg ccggtgaccg
    13021 tgggccggag cggcctctgc tcccggacgc cgctccggcc cgccgggtga aaagaaaaga
    13081 aggggacagc ggacggacat ggccatggct gcggaagaac tggcggcacc gggcggccgg
    13141 gaaatcctgg gaccgcgcgg caggcccctg ctgggaaacg tacgggacta cgagaaggac
    13201 cgcgtcggct ttctgcgggc gaaccacgcg cggtacggcg atttcttccg cttcgacgac
    13261 acgtcggtcg tcgtggccga tcccgacgcc attcgtgacg tccatgtccg caccaacacc
    13321 gatttcctct ccgaggacgc gatcctttcc ggcggccccg cgccgacggg tgagaccagc
    13381 ccgtggatgc gggcccgccg ccccgcctgg aaagccctg
//
