LOCUS       NZ_CP074380            11053 bp    DNA     linear   CON 27-MAY-2024
DEFINITION  Streptomyces sp. MST-110588 chromosome, complete genome.
ACCESSION   NZ_CP074380
VERSION     NZ_CP074380.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. MST-110588
  ORGANISM  Streptomyces sp. MST-110588
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7915503)
  AUTHORS   Booth,T.J., Bozhueyuek,K.A.J., Liston,J.D., Lacey,E. and
            Wilkinson,B.
  TITLE     Bifurcation drives the evolution of assembly-line biosynthesis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7915503)
  AUTHORS   Booth,T.J., Bozhueyuek,K.A.J., Liston,J.D., Lacey,E. and
            Wilkinson,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAY-2021) Molecular Microbiology, John Innes Centre,
            John Innes Centre, Norwich, Not US or Canada NR4, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 28-FEB-2016
            Assembly Method                   :: HGAP v. 3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 25.0x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022695595.1-RS_2024_05_27
            Annotation Date                   :: 05/27/2024 05:38:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,918
            CDSs (total)                      :: 6,828
            Genes (coding)                    :: 6,327
            CDSs (with protein)               :: 6,327
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 501
            CDSs (without protein)            :: 501
            Pseudo Genes (ambiguous residues) :: 0 of 501
            Pseudo Genes (frameshifted)       :: 350 of 501
            Pseudo Genes (incomplete)         :: 185 of 501
            Pseudo Genes (internal stop)      :: 14 of 501
            Pseudo Genes (multiple problems)  :: 45 of 501
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 12:56:23
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1736627
            Orig. end                         :: 1747680
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP074380.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Barrie Wilkinson, John Innes
            Centre, Norwich.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11053
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="12"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..11053
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11053
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:6053](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6053
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    complement(772..786)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 16.24"
                     /tool="antismash"
     misc_feature    943..957
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 17.49"
                     /tool="antismash"
     gene            1023..2018
                     /locus_tag="KGS77_RS07655"
     CDS             1023..2018
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /locus_tag="KGS77_RS07655"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_242579697.1"
                     /transl_table=11
                     /translation="MPTQHRAIQSRQALIRSAAQTFIEKGVPASGMVEISRRARLSKGA
                     LYFHFSSKEDLTLAVRDEALGTLRELEEALVRSPHPARTAIRDFAIALLGRVDADVVMR
                     AGLQLRPETARDMDEAHLEQRWYTLFLDKAAADRNAGRLPPEADPRRIAQLVTSLVVGL
                     LHLGHDDRTWWDKDVITDMWSLLLHAGAPPSEAAGSEAPGSGSDAAPVAGPGPGDARKG
                     AVARTATGTGARTDTGTAVRTGSAAGTDTGGPAGGSGPAVRRAPAGAPRASAPKAPAPK
                     ASDISSTGSATGSATALLAPAVRAAAARTASVTARHGHRAADRSVTTDRS"
     misc_feature    1914..1916
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(2173..3141)
                     /locus_tag="KGS77_RS07660"
                     /old_locus_tag="KGS77_07565"
     CDS             complement(2173..3141)
                     /NRPS_PKS="Domain: PKS_ER (62-320). E-value: 4.7e-22.
                     Score: 70.4. Matches aSDomain:
                     nrpspksdomains_KGS77_RS07660_PKS_ER.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_zinc_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009716513.1"
                     /locus_tag="KGS77_RS07660"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_07565"
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="WP_277994317.1"
                     /sec_met_domain="ADH_zinc_N (E-value: 4.3e-18, bitscore:
                     64.2, seeds: 86, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVSRPEGAPGAGHLAAEEVPVPEPGPGRLLVRNRIMSVTAAMRTM
                     MTESDLPMPAFVPGRALWGPAVGEVVRASGGGFAPGDLVVHPYGWREYAVVEEERAHHA
                     DPAVLPDPAAYLSQGATAWGALTRTAPVRPGDTVFVTGAAGGVGAMAGQIARRLGARRV
                     IGSTGSPHKAERLRTELGYDDIVVRGAGPALEQLRRAAPEGVDVLLDTVGGEQLAAALA
                     VAATGARCALVGALTAQLGGAPAAVRVDPALMVARRITLAGFSRQDHPDLPGEWAAEFG
                     RGLRDGSLVFPHVRLRGIAQAPRALFELTEGRHIGSVLVEV"
     aSDomain        complement(2182..2955)
                     /aSDomain="PKS_ER"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_KGS77_RS07660_PKS_ER.1"
                     /evalue="4.70E-22"
                     /label="KGS77_RS07660_PKS_ER.1"
                     /locus_tag="KGS77_RS07660"
                     /protein_end="320"
                     /protein_start="62"
                     /score="70.4"
                     /tool="antismash"
                     /translation="WGPAVGEVVRASGGGFAPGDLVVHPYGWREYAVVEEERAHHADPA
                     VLPDPAAYLSQGATAWGALTRTAPVRPGDTVFVTGAAGGVGAMAGQIARRLGARRVIGS
                     TGSPHKAERLRTELGYDDIVVRGAGPALEQLRRAAPEGVDVLLDTVGGEQLAAALAVAA
                     TGARCALVGALTAQLGGAPAAVRVDPALMVARRITLAGFSRQDHPDLPGEWAAEFGRGL
                     RDGSLVFPHVRLRGIAQAPRALFELTEGRHIGSVLV"
     CDS_motif       complement(2677..2739)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_KGS77_RS07660_0001"
                     /evalue="1.60E-03"
                     /label="PKSI-ER_m5"
                     /locus_tag="KGS77_RS07660"
                     /protein_end="155"
                     /protein_start="134"
                     /score="11.2"
                     /tool="antismash"
                     /translation="GDTVFVTGAAGGVGAMAGQIA"
     misc_feature    complement(3149..3162)
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 18.85"
                     /tool="antismash"
     gene            complement(3207..3977)
                     /locus_tag="KGS77_RS07665"
                     /old_locus_tag="KGS77_07570"
     CDS             complement(3207..3977)
                     /EC_number="1.1.1.47"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_KR (11-174). E-value: 1.1e-14.
                     Score: 46.8. Matches aSDomain:
                     nrpspksdomains_KGS77_RS07665_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007452957.1"
                     /locus_tag="KGS77_RS07665"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_07570"
                     /product="glucose 1-dehydrogenase"
                     /protein_id="WP_242579700.1"
                     /sec_met_domain="adh_short (E-value: 1.8e-35, bitscore:
                     121.2, seeds: 230, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTDDRRMLDGKTVMITGASSGIGAAAARLFAAEGAAVVLFARREK
                     LLEALAEEIGASGGRAVAVAGDVIRKGDVERAVTTAVERFGRLDGAFNNAGYGTVGTKL
                     HEMDDADFDYVMDVNVRGVWNCLRYETAAMLETGGGSIVNTSSVAGVLATGVGAPYIAA
                     KHAVIGLTKAAASEYAEQGIRVNALAVGNTRTELLQAALDATPGLEETFLARAIQQRLA
                     VPAEVGQAAAWLLCDRSSFITGATVPVDGGWTAI"
     aSDomain        complement(3456..3944)
                     /ASF="KR domain putatively catalyzing L-configuration
                     product formation"
                     /ASF="catalytic triad S,Y,N inconclusive"
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_KGS77_RS07665_PKS_KR.1"
                     /evalue="1.10E-14"
                     /label="KGS77_RS07665_PKS_KR.1"
                     /locus_tag="KGS77_RS07665"
                     /protein_end="174"
                     /protein_start="11"
                     /score="46.8"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="TVMITGASSGIGAAAARLFAAEGAAVVLFARREKLLEALAEEIGA
                     SGGRAVAVAGDVIRKGDVERAVTTAVERFGRLDGAFNNAGYGTVGTKLHEMDDADFDYV
                     MDVNVRGVWNCLRYETAAMLETGGGSIVNTSSVAGVLATGVGAPYIAAKHAVIGLTKAA
                     "
     CDS_motif       complement(3849..3944)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_KGS77_RS07665_0001"
                     /evalue="9.30E-05"
                     /label="PKSI-KR_m1"
                     /locus_tag="KGS77_RS07665"
                     /protein_end="43"
                     /protein_start="11"
                     /score="15.1"
                     /tool="antismash"
                     /translation="TVMITGASSGIGAAAARLFAAEGAAVVLFARR"
     gene            4180..4797
                     /locus_tag="KGS77_RS07670"
                     /old_locus_tag="KGS77_07575"
     CDS             4180..4797
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003981105.1"
                     /locus_tag="KGS77_RS07670"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_07575"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_242579702.1"
                     /transl_table=11
                     /translation="MVKQERALRTRRAVLQAAAEVIGERGYGAATMAEIIQRAGVTKGA
                     LYFHFPSKDALARAVIEEQTDPFVPKETESRLQDAIDFTHEVAIALRDDALLQAGTRIA
                     VETTFSDTPMVPYQEWINIMTEIFGEARRRGELLPSVDPAKAAEFFVSSYTGVQLFSLA
                     ASGRQDLPARVTSLWKHILPGLASPGAMTHVDPQGRPHKTAA"
     gene            5001..6053
                     /locus_tag="KGS77_RS07675"
                     /old_locus_tag="KGS77_07580"
     CDS             5001..6053
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003981106.1"
                     /locus_tag="KGS77_RS07675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_07580"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_242579704.1"
                     /sec_met_domain="AfsA (E-value: 2.6e-23, bitscore: 80.5,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTSAALAAPHDTAGRRAGRVPSRPRVLDMPPLTTTVPREYVHRAA
                     LSEVFLTDWGYAGDDTWVVGAQWPRAHSFYGPAGGLHDPVLLVETIRQAGILLSHVAHG
                     VPLENSIIWQDMRYRLAPEALRTSATPAEIELHVRDEDLVRRGARLAGARQRYRVLRDG
                     AELATATLDFSCHSPAVYRRLRGAYCDLEHAYARRLPPPEPLLPPLVARDRTADVVLSP
                     TGRPHLWQLRVDTAHPVLFDHPVDHTPGMLLVEAARQAAQATAGPGFVLPVEMECAFER
                     YGELDAPCWVRATPRGHDVAGRFRVDIAVEQYDQPVFSARIAARPAGRSAENSFSHSGR
                     GSNGCPLPSN"
     gene            complement(6290..8682)
                     /locus_tag="KGS77_RS07680"
                     /old_locus_tag="KGS77_07585"
                     /pseudo=""
     CDS             complement(6290..8682)
                     /GO_function="GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1087: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010033845.1"
                     /locus_tag="KGS77_RS07680"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_07585"
                     /product="FAD-dependent monooxygenase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MAGEMRTGAALRVAVIGGGPGGLYAAALLKRLDPRRDITVWERNA
                     PDDTFGFGVVLSDETLGGIEHADPVVHRALRREFVRWDDIDIVHRGVTLTSGGHGFAAL
                     GRRRLLAVLHERCRDLGIRLLFRTPAPPAAELAASHDLVVAADGVHSATRAAHADVFRP
                     SLTTHRCRYIWLSADFAFDAFRFEIAETEYGVAQLHGYPYARRTDSRRTDSRRTDPRGP
                     DLGPAPGDATGADASTVIVEMREEVWRRAGLDRSTERESAQWCAKVFSDALYGQPLRTN
                     NSAWTAFRTVVNERWWHGRTVLLGDAAHTAHFSIGSGTKLAVEDALALAACLEEHAALP
                     DALAAYEAERRPVVASTQRAARASLEWFENLAMYLDQPPRRFAFNLLTRSRRVTHDNLR
                     LRDRGFVARVEEEAGVPPGTPPMFTPFELRGLTLRNRVVVSPMDMYSAPDGDGTPGDFH
                     LVHLGARALGGAGLVMTEMVCVSPQGRITPAVRDCTRPDTKRPGAVSPTSYTPRRPGPR
                     SASSSATPAARAPPA"
     gene            complement(8667..9494)
                     /locus_tag="KGS77_RS07685"
                     /old_locus_tag="KGS77_07590"
     CDS             complement(8667..9494)
                     /NRPS_PKS="Domain: ECH (19-267). E-value: 1.5e-50. Score:
                     163.7. Matches aSDomain:
                     nrpspksdomains_KGS77_RS07685_ECH.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1023: enoyl-CoA hydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004982203.1"
                     /locus_tag="KGS77_RS07685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_07590"
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="WP_242579706.1"
                     /transl_table=11
                     /translation="MSPFTGSADTTTTWQHIDVTREDGVVTVTLARPHKLNALTFEAYA
                     DLRDLLAELSRERSVRALVLGGEGRGFCSGGDVEEIIGATLGMDTAQLLDFNRMTGQVV
                     RAVRECPFPVVAALHGVAAGAGAVLALAADFRVADPSTRFAFLFTKVGLSGGDMGAAYL
                     LPRVVGLGHATRLLMLGDVVRAQEAERIGLISELTEEGRAHQAAHALARRLAEGPALAY
                     AQTKALLTAELDMPLAAAVELDAATQALLMNSADYAEFHAAFTEKRPPKWQGK"
     aSDomain        complement(8694..9437)
                     /aSDomain="ECH"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_KGS77_RS07685_ECH.1"
                     /evalue="1.50E-50"
                     /label="KGS77_RS07685_ECH.1"
                     /locus_tag="KGS77_RS07685"
                     /protein_end="267"
                     /protein_start="19"
                     /score="163.7"
                     /tool="antismash"
                     /translation="TREDGVVTVTLARPHKLNALTFEAYADLRDLLAELSRERSVRALV
                     LGGEGRGFCSGGDVEEIIGATLGMDTAQLLDFNRMTGQVVRAVRECPFPVVAALHGVAA
                     GAGAVLALAADFRVADPSTRFAFLFTKVGLSGGDMGAAYLLPRVVGLGHATRLLMLGDV
                     VRAQEAERIGLISELTEEGRAHQAAHALARRLAEGPALAYAQTKALLTAELDMPLAAAV
                     ELDAATQALLMNSADYAEFHAAFTEK"
     misc_feature    9576..9591
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: strong, score: 18.39"
                     /tool="antismash"
     misc_feature    complement(9581..9601)
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 18.94"
                     /tool="antismash"
     misc_feature    complement(9768..9777)
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.16"
                     /tool="antismash"
     gene            9853..10344
                     /locus_tag="KGS77_RS07690"
                     /old_locus_tag="KGS77_07595"
     CDS             9853..10344
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017946673.1"
                     /locus_tag="KGS77_RS07690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_07595"
                     /product="ATP-binding protein"
                     /protein_id="WP_242579707.1"
                     /transl_table=11
                     /translation="MASWRIALPHTTAAVPIARALIRKALAHGGGGESGSEGEGGGAYG
                     ARGGGTPGGAIRTGTDSHTAELLTAELVANAVEHTAGRTPIELVVELRPTGCQVEVHDA
                     DPSPVEGLDPRAPLPRPDPYAENGRGLLLIRTLSSASGCRVTDRGKATWFTLAPGRARS
                     "
     misc_feature    10696..10710
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 17.34"
                     /tool="antismash"
ORIGIN
        1 ccccaccgtg tcccctacat aggcacactc ccacggcggc accccgacct ccgccgccgc
       61 ccgcagcacc agctccgggt gcggcttgcc gcgccggacc acgtcctggc agacgaccgg
      121 cccgagcagg tcgcggatgc ccatcacttc gagcagggtt tcggcgctgc gcgtggcctt
      181 ggaggtggcc acggccagtt ctatgccgtg ggcgcgcagc gcggacagcc cggccgcgac
      241 gccggggaag agcagttggg gaccttcgcc cagaacctcg caggcgaact gctcgcggta
      301 gcgggagatg gcggtcagga cggtgtcatg atcctccggc atccccagga gctggccgaa
      361 ggcggtgccc ggcggcttgc ccatggcgga ggcggcctgt cgggtggtga ccgcgcggcc
      421 ctgctcggag gcgaccttga cgagcaggcg gctgatggcc ggccgggtgt tggcgagggt
      481 tccgtcgaga tcgaagatgg cggctttgat cgtctctgtg ctcatgcccc gcacggcgcc
      541 tccaggacgt acgccgactt ccagtgactc acgcattgct cgacaccttt ccgatttcct
      601 ctggatctgt ggatctatgg gtccgatcgg ggttcgtacg ccctcggaca tgggcagcgt
      661 gtcgaggaca cccgaaggaa gaggcggact cgccgtggta cgccaccgct tcctggtgac
      721 gtgcagccgt cgaggtggca tcgccggaag agacacttcc cgttactcgc ctccgcagat
      781 ttaaaaaccg cctgcgctgt ttgtcaatgc gcagggcgac gctccggtag ccctgggcca
      841 acaattacgg agccccaccg gaccggttaa ctcctcaaca acccttggat tacggcattc
      901 cgcccgctca cgggaacatg ggtgcacctg gatggccggt cagaggcatt ttgagaaccg
      961 cacggttggt ttgtatgtag actacgggcg ctcgtagccg gactagagac aggacggaca
     1021 ccgtgccgac tcagcatcga gccatccaaa gccgccaggc gctcatccgc tcggcggcac
     1081 agaccttcat cgagaagggg gtgcccgcat ccggaatggt ggagatcagc cgccgggcac
     1141 gactgagcaa gggagccctc tacttccact tcagctccaa ggaagacctc acgctcgccg
     1201 tgcgcgacga ggcgctgggc accctgcggg agctggagga ggcactcgta cggtccccgc
     1261 accccgcacg gaccgccata cgggacttcg ccatcgcact gctcggccgg gtggacgccg
     1321 acgtggtgat gcgggccgga cttcagctcc gcccggagac cgcccgcgac atggacgagg
     1381 cacacctgga gcagcggtgg tacacgctct tcctggacaa ggcggcggcc gaccgcaacg
     1441 ccgggcggct gcccccggag gccgaccccc ggcggatcgc ccagttggtg acctcgctgg
     1501 tggtggggct gctgcatctg gggcacgacg accggacatg gtgggacaag gacgtcatca
     1561 ccgatatgtg gagcctgctg ctgcacgccg gggcgccgcc ttccgaagcg gccggctccg
     1621 aagcgcccgg ctccgggtcc gacgccgccc ccgtcgccgg cccgggcccc ggggacgcac
     1681 ggaagggcgc ggtcgcgcgt acggctacgg gtacaggtgc gcgtacggat acgggtacgg
     1741 ctgtgcgtac gggctcggcc gcgggtacgg acaccggcgg gccggccggc ggatccgggc
     1801 cggccgtgcg cagggcgccg gccggcgccc cgagggcctc cgcgcccaaa gcacccgccc
     1861 ccaaggcgtc cgacatctct tcgaccggtt ccgcgaccgg ttccgccacg gcgttactcg
     1921 ctcccgccgt acgggccgcc gccgcccgta ccgcctccgt cacggcacgg cacggacacc
     1981 gggccgccga ccggtcggtc acaacagacc ggtcatgacc tctcggtcac gaccggtgaa
     2041 acgggaagcg ggggcacggt gcgagcagaa cgggggatca ggggcgtaca ggtcgtgagc
     2101 ggatagaagg tgcgcacggc gcgtcggccg accggagttg tccaccgctc ggacagcccg
     2161 gccccggcgc cctcacacct cgaccagtac ggaaccgatg tgccgcccct ccgtcagctc
     2221 gaacagggcg cgcggtgcct gtgcgatgcc gcgcagccgg acgtggggga agaccaggga
     2281 gccgtcccgc aggccccgcc cgaactccgc cgcccactcc ccgggcaggt ccgggtggtc
     2341 ctgccgggag aagcccgcca gcgtgatccg gcgggccacc atcagtgccg ggtccacccg
     2401 tacggctgcc ggggcacccc ccaactgagc ggtcagcgcg cccaccagcg cgcaccgggc
     2461 gccggtcgcg gccaccgcca gcgccgccgc gagctgctcg ccgcccaccg tgtcgagcag
     2521 gacgtcgacg ccctcgggcg ccgcccggcg cagttgttcc agggccggcc cggcgccgcg
     2581 cacgacgatg tcgtcgtagc ccagctccgt acgcagccgt tccgccttgt gcggggagcc
     2641 ggtgctgccg atcacccgcc gcgcgccgag ccggcgggcg atctgtccgg ccatggcgcc
     2701 gaccccgccg gccgcccccg tgacgaacac ggtgtcccct gggcgcaccg gggccgtacg
     2761 ggtcagcgcg ccccaggccg tggcgccctg ggagaggtac gcggcggggt cggggagcac
     2821 cgccgggtcc gcgtggtggg cccgctcctc ctcgacgacg gcgtactcgc gccagccgta
     2881 ggggtgcacg acgagatcac cgggggcgaa gccgccgccg gaggctcgga ccacttcgcc
     2941 gaccgcgggg ccccacagtg cccggcccgg cacaaaggcg ggcatgggga gatcggattc
     3001 ggtcatcatc gtgcgcatgg cggcggtgac ggacatgatc cggttgcgga ccagaagccg
     3061 gccggggccg ggttcgggca ccgggacctc ctcggccgcg aggtgtccgg cgcccggcgc
     3121 gccctcgggg cggctcacca gacgtatctc acggtgggtg gcgggaacgg ctccgccggg
     3181 accgtgggag ctgcggggtc gcacggtcag atcgccgtcc agccgccgtc gaccgggacc
     3241 gtggcgccgg tgatgaacga ggagcggtcg cacagcagcc aggccgcggc ctgtccgacc
     3301 tcggccggga cggccagccg ctgctggatc gcccgggcga ggaaggtctc ctccaggccg
     3361 ggcgtcgcgt ccagggccgc ctggagcagt tcggtgcggg tgttgccgac cgccagggcg
     3421 ttgacgcgga tgccctgctc ggcgtactcg gaggcggcgg ccttggtcag gccgatgacg
     3481 gcgtgcttgg ctgcgatgta cggggcgccg acgccggtgg ccagtacgcc ggcgacgctg
     3541 gaggtgttga cgatggaccc gccgcccgtc tccagcatgg cggccgtctc gtagcgcagg
     3601 cagttccaca ccccgcgtac gttgacgtcc atgacgtagt cgaagtcggc gtcgtccatc
     3661 tcgtgcagct tggtgccgac ggtgccgtag ccggcgttgt tgaacgcgcc gtccaggcgg
     3721 ccgaagcgtt ccacggccgt ggtgaccgcg cgttccacgt cacccttgcg gatgacatcg
     3781 ccggccaccg cgacggcccg cccgccgctc gccccgatct cctcggcgag cgcctccagg
     3841 agcttttcgc gccgggcgaa gagcaccacc gcggcgccct cggcggcgaa caggcgggcc
     3901 gccgccgcgc cgatcccgct ggaggccccg gtgatcatca ctgtcttgcc gtcgagcatt
     3961 cgccgatcat cagtcatggg ggcagtaaaa caggcgccgt gacggccgga aaaaccggcc
     4021 tcggcagccc tttccaacca cttttcaata ccgcgtggtt ggcttatttc gagccgcgaa
     4081 aatagactgc gggtcaagct cggggggatc accgcggatg ccggcccgag gttccgcgag
     4141 agcgacgttc acgacagaac agaagaagga gggtgaccgg tggtcaagca ggaacgtgca
     4201 ctgcgcacac gacgcgccgt cctccaagcg gcggccgagg tcatcggcga gcgcggctac
     4261 ggagccgcca ccatggccga gatcatccag cgcgccgggg tgaccaaggg agccctgtac
     4321 ttccacttcc cctccaagga cgccctggcg cgggccgtca tcgaggagca gaccgacccc
     4381 ttcgtcccca aggagaccga gtcccgcctc caggacgcca tcgacttcac ccatgaggtg
     4441 gccatcgccc tgcgggacga cgcgctgctc caggccggca cccggatcgc ggtggagacg
     4501 accttcagcg acacgcccat ggtgccgtac caggagtgga tcaacatcat gacggagatc
     4561 ttcggcgagg cacgccgacg gggcgagctg ctgccctcgg tggaccccgc caaggcggcc
     4621 gagttcttcg tgtcctccta caccggcgtg cagctcttct cgctggcggc ctcggggcgc
     4681 caggacctgc ccgcgcgggt gacgtcgctg tggaagcaca tcctccccgg gctggcctcc
     4741 cccggcgcca tgacgcatgt cgacccccag ggaaggccgc acaagaccgc cgcctgagtc
     4801 cgcctccggc gccccttcac gctcctgccc ggctgttcac ccgcttccgc ccctgtggcc
     4861 tcccccctga aaatcaggca gcacggactg gaaaacaaac cggctggtct gtatcttggt
     4921 cgccgtcggt ccgccgtcgg tccgccgtcg cgtccatggg cacgggccgc tgagctctac
     4981 ccaccaggga gggaatccgg atgaccagcg cagccctcgc cgcgccgcac gacaccgccg
     5041 gccgccgggc cggccgtgtg ccgtcccgtc cccgcgtcct ggacatgccg ccgctgacga
     5101 cgacggtgcc ccgcgagtac gtacaccggg ccgcgctgtc cgaggtgttc ctgaccgact
     5161 gggggtacgc cggggacgac acatgggtgg tcggcgcgca gtggccgcgg gcgcacagct
     5221 tctacggccc ggccggcggc ctgcacgacc cggtgctgct ggtcgagacc atacggcagg
     5281 cgggcatcct gctcagtcac gtggcccacg gggtgccgct ggagaactcc atcatctggc
     5341 aggacatgcg ctaccgcctg gcccccgaag cactgcggac gtccgcgacc cccgccgaga
     5401 tagagctgca cgtgcgcgac gaggacctgg tgcgccgcgg cgcccggctg gccggcgccc
     5461 gccagcgcta ccgcgtcctg cgcgacgggg ccgaactggc caccgccacc ttggacttct
     5521 cctgccacag cccggccgtc taccgccggc tgcgcggcgc gtactgcgac ctggagcacg
     5581 cctacgcccg gcggctgccc ccgccggagc cgctgctgcc gccgctggtc gcacgcgacc
     5641 gtacggccga tgtggtgctc tcccccaccg gccggccgca tctgtggcag ttgagggtgg
     5701 acaccgcaca ccccgtgctc ttcgaccacc ccgtggacca tacccccggc atgctgctgg
     5761 tggaggccgc ccggcaggcc gcgcaggcca cggcgggccc cggattcgtc ctgccggtgg
     5821 agatggagtg cgccttcgag cggtacggcg agctggacgc gccctgctgg gtgcgggcca
     5881 cgccgcgcgg ccatgacgtc gcgggccggt tccgcgtgga catagcggtg gaacagtacg
     5941 accagcccgt tttttccgcc cgaatcgccg cgcgtccggc aggccgatcg gcggaaaact
     6001 ccttttcgca ttccggccgt ggctcgaacg gctgccccct tccgtcgaat tgaatgccgg
     6061 gcgccgctta gtgccccatt cgacgcgaaa aagccgctgc cccacgcctg aacccgccgc
     6121 cccaatacgg aatggtccgt accttcgttg tccgatgtcc ggagcggcgg gtccggtatg
     6181 ccaggtaccg actccggtat gccgagtacc ggctccggaa tggcgggtcc gggaccaacc
     6241 ggcggcgagg gccgggcgtc accgcggcca cacgtcaccg ggccagatgt caccgcggca
     6301 gggagagccg cggtcgcggc gcgtccgtac ggcccgtggg aggggtccgg ctgcccgccc
     6361 ggtacgggag cggccacggc gcgccgggcc cggcatagtc ctgctcggcg gcggcatgca
     6421 gcgtccagtg cgggtcgtac agatgcgggc gggccagcgc gcacaggtcc gcgcgcccgg
     6481 ccaggaccag tgagttgacg tcgtcccagg aggagatggc gccgaccgcg atgacgggaa
     6541 cgcccacggt gttgcggatc cggtcggcga acggcgtctg gtaggagcgg ccgaagtcgg
     6601 ggcgctcgtc cggcacgacc tgcccggtcg agacgtccag ggcgtccgcg ccgtgcgccg
     6661 cgaaggcgcg cgcgatctcc accgcgtcct ggcaggtcgt gccaccctcg gcccagtccg
     6721 tggcggagat gcggaccgtc atgggccgct cggccggcca cacctcccgt accgcgtcga
     6781 agacctccag ggggtagcgc agccgggcgg cgaggtcacc gccgtaggcg tcgcggcggt
     6841 ggttggtgag cggggagagg aaaccggaga gcagatagcc gtgtgcgcag tgcagttcca
     6901 gcaggtcgaa cccggcccgc gcggcggcct gcgccgaccg tacgaactgc tcgcgcacgg
     6961 tgcgcagttc ggcggcggtc agggcgtgcg ggacctggtt gacaccgggg cggtacggga
     7021 tcggggaggc ggccaccacc ggccagttgt gctccggcag cggctggtcg atgccctccc
     7081 acatcaggcg ggtggagccc ttgcggccgg agtggccgag ctggacgccg atcgcggtcc
     7141 cgggcgcctg ggtgtgtacg aagtcggtga cacggcgcca ggccgcttcg tgtccgggcg
     7201 cgtacagtcc cgtacagccg gggtgatacg gccctgcggg ctgacgcaga ccatctccgt
     7261 catcaccagt ccggcgccgc ccagggcccg cgcgccgagg tggacgaggt ggaagtcacc
     7321 gggggtgcca tcgccgtccg gggcgctgta catgtccatc ggggagacca ccacgcggtt
     7381 gcgcagggtc agtccgcgca gttcgaaggg cgtgaacatg ggcggcgtgc cgggcggtac
     7441 gccggcctcc tcctcgacgc gggccacgaa tccccggtcg cgcagccgca ggttgtcgtg
     7501 ggtgacgcgg cggctgcggg tcaggaggtt gaaggcgaac cggcggggcg gctggtccag
     7561 gtacatcgcc aggttctcga accactccag gctggcgcgg gcggcacgct gggtggaggc
     7621 cacgacgggc cggcgctcgg cctcgtacgc ggccagcgcg tcggggaggg ccgcgtgctc
     7681 ctccaggcac gcggcgaggg cgagggcgtc ctcgacggcg agcttggtgc cggagccgat
     7741 ggagaaatgg gcggtgtggg cggcgtcgcc gagcaggacc gtacggccgt gccaccagcg
     7801 ctcgttgacg accgtacgga aggcggtcca ggcggagttg ttggtgcgca gcggctgtcc
     7861 gtacagggcg tcggagaaga ccttggcaca ccactgggcc gactcgcgtt ccgtactgcg
     7921 gtccaggccg gcccgccgcc agacctcctc gcgcatctcg acgatgacgg tgctggcgtc
     7981 ggcgcccgta gcgtctcccg gggcggggcc gaggtccggg cctcgggggt ccgtacggcg
     8041 ggagtccgta cggcgggagt ccgtacgacg ggcgtacggg tagccgtgga gctgggcgac
     8101 gccgtactcg gtctcggcga tctcgaagcg gaaggcgtcg aaggcgaagt cggcgctgag
     8161 ccagatgtag cggcagcggt gggtggtcag gctcggacgg aagacgtcgg cgtgggcggc
     8221 gcgggtggcg ctgtgcaccc cgtcggcggc gaccaccagg tcgtgggagg cggcgagttc
     8281 ggccgcgggc ggcgccgggg tccggaacag cagacggatg cccaggtccc ggcagcgctc
     8341 gtgcaggacg gccagcagcc ggcggcggcc cagggccgcg aagccgtgtc cgcccgaggt
     8401 gagggtcact ccgcggtgca cgatgtcgat gtcgtcccag cggacgaact cccggcgcag
     8461 cgcgcggtgg acgacggggt cggcgtgttc gatgccgccg agggtctcgt cggagagcac
     8521 gacgccgaag ccgaaggtgt cgtcgggggc gttgcgttcc cagacggtga tgtcccggcg
     8581 cgggtcgagg cgtttgagga gggcggcggc gtacaggccg cccggtccgc ccccgatgac
     8641 ggcgacccgc agggcggcac cggtcctcat ttcccctgcc atttgggggg ccgcttctcg
     8701 gtgaaggcgg cgtggaactc ggcgtagtcg gcgctgttca tcagcagggc ctgggtggcg
     8761 gcgtccagtt cgacggccgc ggccagcggc atgtccagtt cggcggtgag cagcgccttg
     8821 gtctgggcgt acgcgagggc cggcccctcg gccagccggc gggccagggc gtgcgccgcc
     8881 tggtgggccc gcccctcctc cgtcagctcg ctgatcagcc cgatgcgctc ggcctcctgc
     8941 gcgcggacca cgtcacccag catgagcagg cgggtggcgt gccccagacc caccacccgc
     9001 ggcagcaggt acgccgcccc catgtcgccg ccggacagtc cgaccttggt gaagaggaag
     9061 gcgaagcggg tggaggggtc ggcgacgcgg aagtcggcgg ccagcgcgag caccgcgccg
     9121 gctccggcgg cgaccccgtg gagggcggcg accaccggga aggggcactc acgcacggcc
     9181 cggacgacct gtccggtcat ccggttgaag tccagcagtt gcgcggtgtc catgcccagg
     9241 gtggcgccga tgatctcctc gacgtcaccg ccggagcaga agccgcgccc ctcgccgccc
     9301 aggacgaggg cgcgtacgga ccgctccctg gacagctccg cgagcaggtc gcgcaggtcg
     9361 gcgtaagcct cgaaggtcag ggcgttgagc ttgtgcggac gggcgagggt gacggtgacg
     9421 acgccgtctt ccctggtcac gtcgatgtgc tgccatgtgg tggtggtgtc ggcggatccc
     9481 gtgaacgggc tcatgggctg attgccccct tcagccggac ggtcggcggg tgcctggacg
     9541 gtcgctgtcg ctgcccttcg aagttatcac cggtctgtga ctgtcgtcac gagtacgcga
     9601 tagagccgga tgcgtacgcc gtggagccgg cccggcaggg acgcgcgtgg ggcgacaggt
     9661 ggggcacagg cagcacgcag gtgggacaca cgggacatac ggagacgcgc gcggacacac
     9721 gtggacgcgc gcgggagccg aggggacccc gccttccacc gcgccccggc cgccttcgta
     9781 cggtgggggg acccccataa ccccacccgg aaccctcgcg aacggatcag cgctgtgccg
     9841 aaaccttccg ccatggcctc ctggaggatc gccctgccgc acaccacggc cgccgtcccg
     9901 atcgcccgcg ccctgatccg caaggcgctg gcgcacggcg gcggaggcga gagcgggagc
     9961 gagggcgagg gcgggggtgc gtacggcgcc cggggaggcg gcacgccggg cggcgccatc
    10021 cgtacgggga cggacagcca caccgccgag ctgctgaccg cggagctggt cgccaacgcc
    10081 gtggaacaca ccgccggccg tacgcccatc gaactcgtcg tggaactgcg gccgaccggc
    10141 tgccaggtgg aggtgcacga cgccgacccg tcccccgtgg aggggctgga cccccgggct
    10201 cccctgcccc gccccgatcc ctacgccgag aacggccgcg gcctgctgct gatacgtacc
    10261 ctcagctcgg cctccggctg ccgcgtcacc gaccggggca aggccacctg gttcaccctc
    10321 gccccgggcc gggcgcggag ctgaccgcgg ccaccggcgg cccggcgggt gccccttccg
    10381 gggactcgtc cccggacgcg accggggacc cgtccagggg ctcggcccgg gacccgtccg
    10441 cgcctgcggt cgtcgccagc cgggagcgcc ggcgcccgta cgccgagtag accagcgacc
    10501 ccgccaccag gaaagcggcg aactggagcc aggtcgccgg acccgtcccg tagatcaggt
    10561 agaggcagaa ggcgatgccg agcacggggc tgacggggaa gagcgggaca cggaagcggc
    10621 gcgggaggtc gggccgggtc cggcgcagca ccatcacgca cacgttgacg acggccatga
    10681 cggccagcgt cccgatggtc gtcaggttca tcacgacgtc cagagggacg accgcggccg
    10741 gcaccgcgaa gacgaccgcg acgatccagg tgttggcgac gggcgtggag gtccgcgggg
    10801 agatccgttc gaagacccgg ggcaccagtc cgtcccggga catcgacatc aggatgcggg
    10861 tctgcccgta catgacggcc aggacgacgg aggcgatggc cacgaccgcg ccgaaggcga
    10921 tgatcccgcc gccgacggcc gagccggtga cccggtcgac gaccagggag agggccgcgg
    10981 gcttgtcgcc gacctggccg gcgccgagcg cgccgatggc ggtcagggcc accaggcagt
    11041 acaggaccgt gac
//
