LOCUS       NZ_CP074380            11483 bp    DNA     linear   CON 27-MAY-2024
DEFINITION  Streptomyces sp. MST-110588 chromosome, complete genome.
ACCESSION   NZ_CP074380
VERSION     NZ_CP074380.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. MST-110588
  ORGANISM  Streptomyces sp. MST-110588
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7915503)
  AUTHORS   Booth,T.J., Bozhueyuek,K.A.J., Liston,J.D., Lacey,E. and
            Wilkinson,B.
  TITLE     Bifurcation drives the evolution of assembly-line biosynthesis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7915503)
  AUTHORS   Booth,T.J., Bozhueyuek,K.A.J., Liston,J.D., Lacey,E. and
            Wilkinson,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAY-2021) Molecular Microbiology, John Innes Centre,
            John Innes Centre, Norwich, Not US or Canada NR4, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 28-FEB-2016
            Assembly Method                   :: HGAP v. 3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 25.0x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022695595.1-RS_2024_05_27
            Annotation Date                   :: 05/27/2024 05:38:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,918
            CDSs (total)                      :: 6,828
            Genes (coding)                    :: 6,327
            CDSs (with protein)               :: 6,327
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 501
            CDSs (without protein)            :: 501
            Pseudo Genes (ambiguous residues) :: 0 of 501
            Pseudo Genes (frameshifted)       :: 350 of 501
            Pseudo Genes (incomplete)         :: 185 of 501
            Pseudo Genes (internal stop)      :: 14 of 501
            Pseudo Genes (multiple problems)  :: 45 of 501
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 12:56:23
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1662848
            Orig. end                         :: 1674331
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP074380.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Barrie Wilkinson, John Innes
            Centre, Norwich.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11483
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="11"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11483
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11483
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6483](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6483
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(216..893)
                     /locus_tag="KGS77_RS07365"
                     /old_locus_tag="KGS77_07265"
     CDS             complement(216..893)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018523640.1"
                     /locus_tag="KGS77_RS07365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_07265"
                     /product="AIM24 family protein"
                     /protein_id="WP_242579623.1"
                     /transl_table=11
                     /translation="MHSDLFASENMVKPATAPGMTQQNAKSLKYAVDGECYARQGAMVA
                     YRGDLRFEKQGQGVGKFIKRAVTGEGLALMAVRGRGEVWFAHEAANCFIVDLAPGDALT
                     VNGRNVLCFDPSLSYEIKIVKGAGMAAGGLFNSVFTGHGKLGIMCEGNPIVIPVSPQSP
                     VFVDTDAVVAWSASLQTSLHRSQSLGSMLRGGSGEAVQLRLDGEGFVVVRPSEARPEAA
                     AKH"
     misc_feature    complement(893..907)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 18.23"
                     /tool="antismash"
     gene            1144..1920
                     /locus_tag="KGS77_RS07370"
                     /old_locus_tag="KGS77_07270"
     CDS             1144..1920
                     /GO_function="GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018491671.1"
                     /locus_tag="KGS77_RS07370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_07270"
                     /product="peptidyl-tRNA hydrolase"
                     /protein_id="WP_242579624.1"
                     /transl_table=11
                     /translation="MTSTASNDHPVPPSGTADGDGPAEAFRGEASDRDEAPQFVLPLVV
                     RMERADPPARTDALETAARAVLVLLSDERATGDGEWAAAVRDWQDARIRKVVRRARGAE
                     WRRAQALPGITVTGERAEVRVFPPVPLNGWPKELARLQVSGTELDDPRPAAAPDPDRPV
                     LWLNPGLKMSAGKAMAQTGHGAQLAWWELSEAERTAWREAGFPLSVRVAETTAWRRLTH
                     SGLPVVRDAGFTEIAPGKTVVAEGGSRYCPLPGGRP"
     gene            complement(2104..2496)
                     /locus_tag="KGS77_RS07375"
                     /old_locus_tag="KGS77_07275"
     CDS             complement(2104..2496)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018569556.1"
                     /locus_tag="KGS77_RS07375"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_07275"
                     /product="darcynin family protein"
                     /protein_id="WP_242579626.1"
                     /transl_table=11
                     /translation="MSVTSATSVTSVEETAPPVTAFMLIKTMPEWLAMTPQERVNAFAT
                     EVMPAVQAKTKGVRSRFYDTEFYSTRVTDVWVWEADDHNAYQLLIDALRETPFWDRYFE
                     VVDLLVGTENGYARTYGVDPITTITT"
     gene            2643..3596
                     /locus_tag="KGS77_RS07380"
                     /old_locus_tag="KGS77_07280"
     CDS             2643..3596
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1125: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020548163.1"
                     /locus_tag="KGS77_RS07380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_07280"
                     /product="LysR family transcriptional regulator"
                     /protein_id="WP_242587338.1"
                     /transl_table=11
                     /translation="MTLVNNAEPVIDAQLAVALDALLTEQSVTRAAARLHTSPAAMSRT
                     LARLRRVLQDPLLVRAGQTMVPTPRAQALRDEAAAVVRRLGALLGPGTGVDPADLRTTF
                     TLQAADLVGAALAPGLLRLARQEAPGVSLRFRAEELEAGPALRDGHIDLEVGAIDHVDP
                     ETQVEELVTLRMTAAVRPGHPLAGKTLTPARLAAAEHVAVSRRGRFTGPLDTALAEHAL
                     HRRVTAVLPSHLAAMALAARSDVVCLIPTAPPGAAPSPITDAATTLGLRLLDIPLPLPP
                     VTIGMAWHPRHAADGAHHWLRDAVRRVLRAPGSPTA"
     gene            complement(3726..4658)
                     /locus_tag="KGS77_RS07385"
                     /old_locus_tag="KGS77_07285"
     CDS             complement(3726..4658)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF019976.4"
                     /locus_tag="KGS77_RS07385"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_07285"
                     /product="nuclease-related domain-containing protein"
                     /protein_id="WP_242579628.1"
                     /transl_table=11
                     /translation="MDEWRNRAADGTGSGGSEEDRESREGRESRERRERQERRERRTGR
                     ESRASRENRESRENRESRVSREDRGRPDDGRLSRSREVTDWEAKQETHYATDPDRLFLP
                     PDDDLAPNRPGETLRARLAEHPRAGLPLLLARLLGRHPPQDAWRGGLAGIQAVGAELDR
                     LSPGGWYVLHSVPLPPDTGIDHLLIGPGGVLCVSTHYVRKAYVWVGEEVVRAGRDRQPQ
                     PYVRWCRHNARRASYVLTRGCGFRVQARPVLAFVGAARVETDPALRDVQVIPDERGIAA
                     LGTLGGVLAPARIERIHTIARNRRTWLPA"
     gene            5001..6483
                     /locus_tag="KGS77_RS07390"
                     /pseudo=""
     CDS             5001..6483
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018888544.1"
                     /locus_tag="KGS77_RS07390"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="DUF692 domain-containing protein"
                     /pseudo=""
                     /sec_met_domain="DUF692 (E-value: 2.3e-96, bitscore: 320.8,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRHLGTGIGWRPEIATEIAALPGIDWVEVVAENICPGHLPPALQE
                     LRDRGTTVVPHGVSLGLGGADRPDAGRLAALAARAEALDAPLVTEHIAFVRAGGPLTAS
                     PPMEAGHLLPVPRTRDALDVLCENVRIAQDALPVPLALENIAALINWPDEEMTEGQFLA
                     ELVRRTGVRLLIDVANLHTNHVNRNEDPAVALDELPVEAIAYVHVAGGVERDGVWHDTH
                     AHPVTRPVLDVLGELCARVTPPGVLLERDEDFPDGTELAAELDAIRAVVTEVRHAAAA"
     gene            6480..7547
                     /locus_tag="KGS77_RS07395"
                     /old_locus_tag="KGS77_07300"
     CDS             6480..7547
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014175676.1"
                     /locus_tag="KGS77_RS07395"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_07300"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_242579630.1"
                     /transl_table=11
                     /translation="MTFAPLMFLAVLLSSLLLVIATVRFRWTPVKHRVSTWRSTPRGRD
                     LWESAFLAGGPGRVADVAITAMHQDRRLSIGGPGAVSVRRPEANGAVEEAVLEALDRIP
                     GGSLAAVRHAVMHSPAVQQIGDRLADLGLLRRPGPARPWRRLATVQIWLCVAAFAVSLV
                     LTVTGTVGHGPWELPYVWKTVPGAVLGVVVGAICRRALKRRLTEAGADALRAFRNKHRD
                     IRKGAGPTAAIGVALGGTAVLTVKSLRDQLTEAQLATGAAASGSSRSWWPSSGSSSGSA
                     SGSSDSSSTIWFLTNDSGAAWCGSSDGGSSCGGSSCGGGSSCGGSSCGGSSCGSSSGGS
                     SCSSSSCSSSSCGSS"
     gene            7683..>8426
                     /locus_tag="KGS77_RS07400"
                     /old_locus_tag="KGS77_07305"
                     /pseudo=""
     CDS             7683..>8426
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010983659.1"
                     /locus_tag="KGS77_RS07400"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_07305"
                     /product="DUF4142 domain-containing protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MRSLTGTGLVVLALLATLTALLFPIWSFANGTEGAGGTAGGAATQ
                     TVATQFGPLSALDRDFVARVRLAGLWELPAGQQAQERGTAQAVREAGDHLVQGHTELDR
                     RVREVAARLGLDLPSQPNEQQRHWLSELSAAHGPSYDRKFANILRLAHGKVFAVVAQVR
                     ATTRNSLVRGLADDANTTVLDHISVLEQTGLVDFDAIARDAATASPTPPPSAPPPSGGA
                     VPAAPPLRATPSVTYSLPPAASFPAP"
     misc_feature    8872..8885
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 18.52"
                     /tool="antismash"
     misc_feature    complement(8873..8886)
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 19.66"
                     /tool="antismash"
     gene            8882..9820
                     /locus_tag="KGS77_RS07405"
                     /old_locus_tag="KGS77_07310"
     CDS             8882..9820
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003984743.1"
                     /locus_tag="KGS77_RS07405"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_07310"
                     /product="DUF1996 domain-containing protein"
                     /protein_id="WP_242579633.1"
                     /transl_table=11
                     /translation="MTHPTDNHPAHGGPAAEDFADIRSVRPSVQRPPNGPGASTGTFTS
                     RCGRNENGHNNPDNFIVTPGVTNGAHHLHDYVGNLSTDAFSTDASLRAAGTTCAQGDRS
                     TYFWPVLRLRDDRGRQPGANGSANGNASGNADGNVGRALTPRDVSLTFLGNPAGKVTAM
                     PEALRVITGDAKAFTNGTANARAQWTCTGFEDRRLSDKYPLCPGGSQVVRLLDFPSCWD
                     GTSTDSANHRTHIVFPDRVTGRCPAATKAVPQLRMRLTYSVPARPLAFAVDSFPEQLHK
                     PVTDHADFADFMPRSLMAAAVRCINSGRRCG"
     misc_feature    complement(11144..11158)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 16.9"
                     /tool="antismash"
ORIGIN
        1 cgggaagacg gggcggaagg cgtgagagaa gaggggaaga cgtggcgatc atccgcgcca
       61 ccgtgccgcc ggacgcggcg gccggctcgg cctgcgcccc ctgagacggg gacacaggca
      121 ctccgacggg agcggggaac gccggggaag ctcaggtctc agagaaaggc cggcgggagc
      181 gcgggcactc cggcgagagt caggctctca gtccctcagt gcttggccgc cgcctcgggg
      241 cgggcttcgc tggggcgtac gaccacgaag ccctcgccgt cgagccggag ctgcaccgcc
      301 tcgccggagc cgccgcgcag catcgagccc aggctctgcg agcggtgcag ggaggtctgg
      361 agggacgcgc tccaggccac cacggcgtcg gtgtccacga agaccgggga ctgcggcgag
      421 acggggatga cgatggggtt gccctcgcac atgatgccga gtttgccgtg cccggtgaag
      481 acggagttga acagtccgcc ggcggccatc ccggcaccct tcacgatctt gatctcatag
      541 gagaggctgg ggtcgaagca caggacgttg cggccgttga cggtgagggc gtcaccaggt
      601 gcgaggtcga cgatgaaaca gttggcggct tcgtgggcga accacacctc gccccgcccg
      661 cgcacggcca tcagcgccag gccctcgccg gtgacggcgc gcttgatgaa cttgcccacg
      721 ccctgcccct gcttctcgaa ccgcaggtcg ccgcggtagg cgaccatcgc tccctggcgc
      781 gcgtagcact cgccgtcgac ggcgtatttg agggacttgg cgttctgctg ggtcatcccg
      841 ggcgcggtgg ccggcttgac catgttctcg gaggcgaaga ggtcgctgtg catggacatg
      901 ataatcgccc ggattgccac gatccgccag ttcagttcca cgcatgggtt ccacgtgtgc
      961 cgtccgcgcg ccgcgccgtc cgtacatgac atgtgctgcg cggtccgtac gacacacgtc
     1021 ggtccgtaca tccgcacgtc cgtacagtcc gtacggtccg tacgccccac cgtcacagcg
     1081 ccccaccgcc cttcgcgccc ggccgccgcg ggcaccgtcg gcgcaggctg gcagactggc
     1141 cgggtgacca gcacagccag caacgaccac cccgtccctc cctccgggac ggccgacggc
     1201 gacggcccgg cagaggcgtt ccgcggggaa gccagcgacc gggacgaggc gccccagttc
     1261 gtgctcccgc ttgtcgtacg gatggagcgg gccgatcccc cggcacgtac ggacgcgctg
     1321 gagacggcgg cacgggccgt cctggtgctg ctgtcggacg agcgcgcgac cggggacggc
     1381 gagtgggccg cggccgtacg ggactggcag gacgcccgta tccgcaaggt cgtacggcgg
     1441 gcccgcggcg cggagtggcg gcgggcacag gccctcccgg ggatcacggt caccggcgaa
     1501 cgggcggagg tacgggtctt cccgccggtg ccgctgaatg gctggccgaa ggaactggcc
     1561 cgattgcagg tctcgggcac cgagctggac gacccgcggc ccgccgccgc gccggacccc
     1621 gaccggcccg tgctgtggct caaccccggc ctgaagatgt cggcgggcaa ggcgatggcc
     1681 cagaccggtc acggcgcgca gctcgcatgg tgggaactgt ccgaggccga gcgcacggcc
     1741 tggcgcgagg ccggcttccc cctgtccgta cgcgtggcgg aaacgacggc ctggcgccgc
     1801 ctgacacaca gcggactccc cgtggtccgg gacgcgggtt tcaccgagat cgcgcccggg
     1861 aaaaccgtgg tggccgaggg aggcagtcgc tactgccctc tccccggtgg acgcccgtag
     1921 gcggtcgcct gcgcccggaa cgcacggccg ggcttgttgc accacatatc gggcctgttg
     1981 caccacctat gcggaccggg ctcgccacga cggccttgcc cgcaggcgcg gtcttcgcgt
     2041 gtacgggtgt gcgggcaagg ccgtcggtcc atcgctcctg ctcactcgtc cgcaggggcc
     2101 gtatcaggtg gtgatggtgg tgatggggtc gacgccgtag gtgcgggcgt agccgttctc
     2161 ggtgccgacg aggaggtcga cgacctcgaa atagcggtcc cagaacggtg tttcgcgcag
     2221 ggcgtcgatg aggagctggt aggcgttgtg gtcgtcggcc tcccagaccc agacgtcggt
     2281 gacgcgggtg gagtagaact ccgtgtcgta gaagcgtgac cggacgccct tggtcttggc
     2341 ctggaccgcg ggcatgacct cggtggcgaa ggcgttcacc cgttcctggg gggtcatggc
     2401 gagccactcg ggcatggtct tgatgagcat gaaggcggtg accggcggtg cggtttcctc
     2461 gacggatgtg acggacgtgg cggacgtgac ggacatggcg ggtgcctcca aggtggcggg
     2521 ttcaggatga cggcgggtca caagacctcg cgggtcgtac cgagtggtac gacctcatcc
     2581 tgcggagggc cgcggtgttg ccacaatggg aacttcagca acggatcatt gcctgaagtg
     2641 gaataaccct ggtgaacaat gcagagccgg tcatcgacgc ccaactcgcc gtcgcgctgg
     2701 acgccctgct gaccgaacag agcgtcaccc gcgccgccgc gcgcctgcac acctcccccg
     2761 ccgccatgag ccgtaccctc gcccgtctgc gccgcgtcct ccaggacccc cttctggtcc
     2821 gggccggaca gaccatggtt cccacgcccc gcgcccaggc cctgcgcgac gaggccgccg
     2881 cggtggtgcg ccgactggga gcgctgctcg gtcccggcac cggcgtcgac cccgcggacc
     2941 tgcgcaccac cttcaccctc caggccgccg acctggtcgg cgcggcactg gcccccggac
     3001 tgctgaggct ggcccggcag gaggcgcccg gggtttcgct gcggttccgg gccgaggagc
     3061 tggaggccgg gccggccttg cgcgacggcc acatcgacct ggaggtcggg gccatcgacc
     3121 acgtcgaccc cgagacgcag gtcgaggaac tggtcaccct ccggatgacg gcggccgtcc
     3181 ggcccggcca ccccctcgcc ggaaagaccc tgaccccggc ccggctcgct gccgccgaac
     3241 atgtcgcggt cagccggcgc ggccggttca ccggccccct ggacaccgcc ctggccgagc
     3301 acgcccttca ccggcgggtc accgcggtcc tccccagcca cctggccgcg atggccctcg
     3361 ccgcccgcag cgacgtcgtc tgtctcatcc ccacggcacc tcccggcgcc gccccctcgc
     3421 ccatcaccga cgccgccacc accctcggcc tgcgcctcct cgacattccc ctgccgctgc
     3481 cgcccgtgac catcggcatg gcctggcatc cccgccacgc cgccgacggg gcccaccact
     3541 ggctccgcga cgccgtccgc cgcgtccttc gcgcccccgg cagcccaact gcctgacgcg
     3601 gccgggcccc ttcggctgcg gggcagccgc gaccggggag gagtacgcgc ccgggtcctg
     3661 cgcggccgtc ggggctgtcg gggtcgtcgg ggccgactgt tacgtggtgc gcacggtcgg
     3721 ccgtttcagg cgggaagcca ggtgcggcgg tttctggcga tggtgtggat gcgttcgatc
     3781 cgggcggggg cgagtacgcc gcctagcgtt cccagggcgg cgatgccgcg ctcgtcgggg
     3841 atcacctgta cgtcccgcag cgccgggtcg gtctccaccc gtgcggcgcc cacgaacgcc
     3901 agtaccgggc gtgcctggac gcggaatcca cagccacggg tcaggacgta cgacgcgcgc
     3961 cgggcgttgt ggcggcacca gcgcacgtac ggctgcggct ggcggtcgcg gccggcccgg
     4021 acgacctcct caccgaccca tacgtacgcc ttgcggacat agtgcgtgct gacacacaga
     4081 acgccgccgg ggccgatgag cagatggtcg atgccggtgt cgggcggcag cggtacggag
     4141 tgcagcacgt accagccgcc gggcgagagg cggtccagct ccgcaccgac ggcctgtatg
     4201 cccgccaggc cgccgcgcca ggcgtcctgc ggcggatggc ggcccaggag acgggcgagg
     4261 aggagcggga gtccggcgcg cgggtgctcg gccaggcggg cgcggagcgt ctcgccgggg
     4321 cggttcggcg cgaggtcgtc gtccggcggc aggaacagcc ggtccgggtc ggtcgcgtag
     4381 tgcgtctcct gtttcgcttc ccagtcagtc acctcccggg aacggctcag gcggccgtcg
     4441 tcggggcggc cccggtcttc gcggctcacc cggctctcgc ggttctcccg gctctcgcgg
     4501 ttctcccggc tcgctcggct ttccctgccg gtccggcgtt cgcggcgttc ctggcgttcg
     4561 cggcgttcgc gactctcccg gccttcccgg ctttcccggt cttcctcgct gccgccggaa
     4621 ccggtgccgt ccgcggccct gttgcgccac tcgtccatgc cccaccaccc cgagggccat
     4681 agcagcagcc tcccggcccc cgtgcaagag ggacaaacat ttggctcccc ggaccgcgtg
     4741 caccgagccg tccactcctt gggcatcagc gcgtacgcga acgggtcgtc gcaggtcagg
     4801 gggatggagc aggggcgggg tgagtatgag gggcgcggtc gcgggacgta cggtgggatc
     4861 tgcgtccggc cctcggacgg ccaccaagct caaatcaacc tctgaaccgt ccgccccgag
     4921 ggttggcccg caccggacgg ggccatcaag taccggcaca ccgtccacgg cacccgtgcc
     4981 acgcagtgaa agggggagtc atgcggcacc tgggaaccgg catcggctgg cggccggaga
     5041 tcgccaccga gatcgcggcc ctgcccggca tcgactgggt ggaggtcgtg gccgagaaca
     5101 tctgtccggg tcatctcccg cccgcccttc aggaactgcg tgaccggggc accaccgtcg
     5161 tcccccacgg cgtctcgctc gggctcggcg gcgcggaccg gccggacgcc ggccggctgg
     5221 ccgccctcgc cgcccgcgcc gaggccctgg acgcgccgct ggtcaccgag cacatcgcgt
     5281 tcgtacgggc cggcggcccg ctcaccgcct caccgccgat ggaggccggg cacctgctgc
     5341 ccgtgccgcg cacccgcgac gcgctggacg tgctgtgcga gaacgtccgt atcgcacagg
     5401 acgcgctgcc cgtaccgctt gcgctggaga acatcgccgc cctgatcaac tggccggacg
     5461 aggagatgac cgaggggcag ttcctggccg agctggtacg gcggaccggt gtacggctgc
     5521 tcatcgacgt cgccaacctc cacaccaacc acgtcaaccg gaacgaggac ccggccgtgg
     5581 cactggacga gctgccggtc gaggccatcg cgtacgtcca tgtcgcgggc ggcgtcgaac
     5641 gggacggcgt ctggcacgac acccacgccc acccggtgac acggcccgtc ctggacgtgc
     5701 tcggcgagct gtgtgcccgc gtcacaccgc ccggggtgct gctggagcgc gacgaggact
     5761 tcccggacgg cacggagctc gccgccgaac tggacgcgat acgcgcggtg gtgacggagg
     5821 tacgccatgc cgctgccgcc tgagccggat acccagaggg ccgacgtacc ggaggccggt
     5881 atatcggaga ccgaagtacc ggaggccggc atatcgggga ccgaagcatc ggggaccggc
     5941 atatcgggga tcggtacgcc gaggaccgag acgcccgggc ccggtacgcc aaaggctcgt
     6001 ggaccggagc ccggtacgcc tcggactcgc acgacagacg cccccgcatc cggcaccgac
     6061 gccgcgcggc agcggctggc gctggcgcag actgcgctgc tgtccgccct cgtcgccggc
     6121 accccggccc cggagggctt cgaccgccgc cggctgcgcg tacagagccg ggcgctggcc
     6181 gccaagcgtg cctccgtggt cgccaaggtg gccccggagc tgccggagat cctcggcgcg
     6241 gactaccggg actccttcct cggctatgcg ttgcaccggc ccctgcacgg cggctaccgg
     6301 caggacgcgc tgaacttcgc ggcccatctg ctggccgggg acctgcccgc ggacccggcc
     6361 gcccggcggc ggctcgcgca gtggtggcgg gaccgggcgg gtccccggcc gccctcgtcc
     6421 cgcccctggg cccgattcct gcgagccgca cggctcgccc cgcaaagaaa gcaccactcg
     6481 tgaccttcgc accactgatg ttcctggcgg tcctcctctc gtcgctgctg ctcgtgatcg
     6541 ccacggtccg cttccgctgg acgcccgtga agcacagggt gtccacctgg cgcagcacgc
     6601 cgcgtggccg ggacctgtgg gaatcggcgt tcctggccgg cggccccgga cgggtcgccg
     6661 acgtcgcgat caccgccatg caccaggacc ggcggctgtc catcggcggc cccggcgcgg
     6721 tgtcggtacg gcggcccgaa gcgaacggcg cggtcgagga agccgtcctc gaagcgctgg
     6781 accgtattcc cggcggctcg ctggccgcgg tgcgccacgc cgtcatgcac agcccggcgg
     6841 tccagcagat cggtgaccgg ctggcggacc tggggctgct gcgccgcccc ggccccgccc
     6901 ggccgtggcg gcggctggcg accgtccaga tctggctgtg tgtggccgcc ttcgccgtca
     6961 gcctggtact gaccgtcacc gggacggtgg gccacgggcc ctgggagctt ccgtacgtct
     7021 ggaagaccgt gccgggggcc gtcctggggg tggtcgtcgg cgcgatctgc cgacgcgccc
     7081 tgaagcggcg gctgaccgag gccggcgccg atgccctgcg cgcgttccgg aacaagcaca
     7141 gggacatcag gaagggcgcg ggtcccaccg ccgccatcgg cgtggccctc ggcggcacgg
     7201 ccgtgctcac ggtcaagtcg ctgcgcgacc agctcaccga ggcgcagctc gccacgggcg
     7261 ccgccgcctc cggctcctcc cggtcctggt ggccctcctc gggctcctcc tccggctcgg
     7321 cctcgggctc ctccgacagc agctccacca tctggttcct gaccaacgac tccggcgcgg
     7381 cgtggtgcgg ctcgtccgac ggcggttcga gctgtggcgg gtcgtcctgc ggtggcggtt
     7441 cgtcgtgcgg cggttcgtcg tgcggcggct cgtcgtgcgg cagttcgtcg ggcggttcca
     7501 gttgcagcag ttcgagttgc agcagttcga gctgcgggag tagctgagtc gccaaggagc
     7561 gcgcggggcc cgggcaaccg gtcaacgtac cagcggggga cggccgttga gcgggcttcc
     7621 ccgaagggga gacaggcgtg ccccagggcg cactagcgtg cggtgccgca tcaggaggca
     7681 tcatgcgatc ccttaccggc accggactcg tcgttctcgc actgctcgcg acgctcaccg
     7741 ccctgctgtt ccccatctgg tccttcgcga acggtacgga aggcgccggc gggacggccg
     7801 gcggcgccgc cacccagacc gtcgccaccc agttcggccc gctgtccgca ctcgaccgcg
     7861 acttcgtcgc acgggtgcgg ctggccgggc tgtgggagct gcccgccggc cagcaggccc
     7921 aggagcgcgg tacggcccag gccgtgcgcg aggcgggcga ccatctcgtg cagggccaca
     7981 ccgaactcga ccggcgggta cgggaggtgg ccgcccggct cggcctcgac ctgcccagcc
     8041 agcccaacga gcagcagcgg cactggctgt ccgagctgag cgccgcgcac gggccctcgt
     8101 acgaccggaa gttcgccaac atcctgcggc tggcgcacgg caaggtcttc gcggtcgtcg
     8161 cccaggtccg cgccaccacc cgcaactcgc tcgtccgggg tctcgcggac gacgccaaca
     8221 ccacggtcct tgaccacatc tccgtcctcg aacagaccgg gctcgtcgac ttcgacgcca
     8281 tcgccaggga cgccgccacc gcctcgccga cgccgccccc ttccgcaccg ccgccctccg
     8341 gtggcgcggt cccggccgcc ccaccgctgc gtgccacacc ctcagtgacg tattcgctgc
     8401 ctccggccgc ctccttcccg gcccctcccc ggaaccggat ccccccgcca ccggggccgg
     8461 gacgggggcc ggggccggga cgggggccgg ggccgggacc gcccgcgcgt cccaggacgc
     8521 ctccggaccg gccggttccg ccgcccggcc acatcagcag gacgaacccg accagccccc
     8581 acaggacgag aagaagtgac cgcccatgcc aggccatcag cccacccccc gccaccgtcg
     8641 ccgcctcggc gcccagcatc tcgtgctcct cgcgctcttc gcgctgctcg gctccgccgg
     8701 tgtcttcgtc tccgtcacct cgaacgcatc ggctcgcaac gccgcctccc cttctgccag
     8761 ttcttccggc cctgccggtt ctgccagttc ttccggccct ggcgccgatt ccggttccag
     8821 ccccgcttcc cctcccgctc ccccgtcccg ggacacctct cccacaccgt cgttacccag
     8881 cgtgacacac cccactgaca accaccccgc gcacggcggt ccggccgccg aggacttcgc
     8941 cgacatccgc tccgtacgcc cctccgtaca gcgcccgccg aacgggcccg gcgcctcgac
     9001 cggcacgttc accagccgct gcggacgcaa cgagaacggc cacaacaacc ccgacaactt
     9061 catcgtcacc ccgggtgtca ccaacggcgc ccaccacctc cacgactacg tcggcaacct
     9121 gtccaccgac gccttctcca ccgacgcctc gctgcgcgcg gccggtacca cctgcgccca
     9181 gggcgacagg tccacgtact tctggcccgt gctgcgcctg cgcgacgacc ggggccggca
     9241 gccgggcgcg aacgggagcg ccaacgggaa cgcgagcggg aacgcggacg ggaacgtggg
     9301 ccgcgcgctg accccacggg acgtctccct caccttcctc ggcaacccgg ccgggaaggt
     9361 caccgcgatg cccgaggcgc tgcgcgtcat caccggtgac gccaaggcgt tcaccaacgg
     9421 gaccgccaac gcccgcgccc agtggacctg cacgggcttc gaggaccgcc ggctgagcga
     9481 caagtacccg ctgtgccccg gcggcagcca ggtcgtgcgc ctgctggact tccccagttg
     9541 ctgggacggc acctcgacgg acagcgccaa ccaccgcacc cacatcgtct tcccggaccg
     9601 cgtcaccggc cgctgccccg ccgccaccaa ggccgtcccc cagttgcgga tgcggctgac
     9661 ctactccgtc cccgcccggc cgctggcctt cgcggtcgac agctttcccg aacagctcca
     9721 caagccggtc accgaccacg ccgacttcgc cgatttcatg ccgcgaagcc tgatggcggc
     9781 ggccgtacgc tgcatcaact ccggccgccg ctgcggctga cgaggccacc gctgcggctg
     9841 acggaaggcc ggaaggggcg acgggggccg ggccggaaag gccgggcccc tctgcttcgt
     9901 cccatgcccc cctgctttgc cccacgcccc tctagttccg tcccggcccg gtacgccata
     9961 ccaccggttc ccgcccacgg caccttggcg ataacccgat cggacggggg cggccggccc
    10021 ggcgacggct cgcgggtgtc gcgtacccgc cccgccgtac gcggcccggc cgcacccgcg
    10081 cgttccgttc cgcgccccgt actccactcg tccggggcat agcctccgac accggcgccc
    10141 ggtgctttac ttcatcaaca ctcgcgccaa ttatcccttt tgtatgcaca ccctcgccgg
    10201 gcttcacggg ccccgcgccg cacgaaggga cggcatgtga gagcagcagc gttgtacggc
    10261 accgtcggat cactggtcgt gaccgcactg gtcgccttcc ctgccgacgg ggccgccgcg
    10321 cccgtcccgc cggcgccggc gcccccgatg gcggctccgg cgtactacac ccccaccccc
    10381 ctgaacacac cgccgacgac gcccaccact ccacggcccc ccacggtgac gccgacccgc
    10441 cccccggtcc agaccccggc ccccacctcc tcggcgacga gcccggtccg tccgcccatg
    10501 aaggacccgg tcaactcccc cagtcccgcg gtcgccccgt ccacggccaa gccgctcccg
    10561 gtcaggccgc ccacggtcac cctgcccgcg gtcgccgtac cgcccgcggc ccggccgccc
    10621 ctccccgtcg cccggcgcat cgccttcggc cgctgcgcgc ccgtggagca cctgccctcc
    10681 gtcgtggagt gcgggaaact caccgtgccc ctggactacg cccaccccga cggcaagaag
    10741 atcgatctga ccgtcagccg gctgcgctcc aaggggacgg ccgcggagcg ccagggcgcc
    10801 ctggtctaca accccggcgg tccgggcgcc tccagcatga agttccccat gtacagcgcg
    10861 atcggcacct tccgcaaact cctggacgcc tacgacttcg tgggctacgc gccacgcggc
    10921 gtcgaccgct ctgcgcccct gtcctgccag aagccggcgg aattcgccaa ggcgcccacc
    10981 gacagcccgc gcgtcccttc ggagcgctac aagcggcagc ggaccctcca ggcggcggcg
    11041 tacgcgcgcg gctgcgcccg taacgcgggc cggtccctgc gccacttcac gtccctgaac
    11101 aacgcccgtg acctggacat gctgcgggcc gggctgggcg agaagaagct gacgttcatg
    11161 ggcgcctcct acgggacgta cttcggcgcc ctgtacgcga cgctcttccc cgggcatgtc
    11221 cgccggatgg tcttcgacag cgtcgtcaac cccgatcccc ggcagatctg gtaccgcagc
    11281 aacctcgacc agaacatcgg cttcgagcgc cgctggcgcg actggcgccg gtgggtggcc
    11341 aagcacgaca agacctacca cctcggcacg acggagcagg ccgtgcaccg ctcgtacgag
    11401 accgcccggg accggctgcg cggcaaaccc gcgggcggca aggtcggccc gggacagttg
    11461 caggcggcct tcctgaagac ggg
//
