LOCUS       NZ_CP074380            22236 bp    DNA     linear   CON 27-MAY-2024
DEFINITION  Streptomyces sp. MST-110588 chromosome, complete genome.
ACCESSION   NZ_CP074380
VERSION     NZ_CP074380.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. MST-110588
  ORGANISM  Streptomyces sp. MST-110588
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7915503)
  AUTHORS   Booth,T.J., Bozhueyuek,K.A.J., Liston,J.D., Lacey,E. and
            Wilkinson,B.
  TITLE     Bifurcation drives the evolution of assembly-line biosynthesis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7915503)
  AUTHORS   Booth,T.J., Bozhueyuek,K.A.J., Liston,J.D., Lacey,E. and
            Wilkinson,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAY-2021) Molecular Microbiology, John Innes Centre,
            John Innes Centre, Norwich, Not US or Canada NR4, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 28-FEB-2016
            Assembly Method                   :: HGAP v. 3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 25.0x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022695595.1-RS_2024_05_27
            Annotation Date                   :: 05/27/2024 05:38:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,918
            CDSs (total)                      :: 6,828
            Genes (coding)                    :: 6,327
            CDSs (with protein)               :: 6,327
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 501
            CDSs (without protein)            :: 501
            Pseudo Genes (ambiguous residues) :: 0 of 501
            Pseudo Genes (frameshifted)       :: 350 of 501
            Pseudo Genes (incomplete)         :: 185 of 501
            Pseudo Genes (internal stop)      :: 14 of 501
            Pseudo Genes (multiple problems)  :: 45 of 501
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 12:56:23
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 512602
            Orig. end                         :: 534838
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP074380.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Barrie Wilkinson, John Innes
            Centre, Norwich.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..22236
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="lassopeptide"
                     /region_number="6"
                     /rules="(PF13471 and Asn_synthase or micJ25 or mcjC)"
                     /tool="antismash"
     cand_cluster    1..22236
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /kind="single"
                     /product="lassopeptide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..22236
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:12236](+)"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..12236
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
     misc_feature    complement(296..306)
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 18.59"
                     /tool="antismash"
     gene            301..747
                     /locus_tag="KGS77_RS02265"
                     /old_locus_tag="KGS77_02210"
     CDS             301..747
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010982100.1"
                     /locus_tag="KGS77_RS02265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_02210"
                     /product="hypothetical protein"
                     /protein_id="WP_242578435.1"
                     /transl_table=11
                     /translation="MATESTVTARARANVPRGSTLLQVAIGLQTLTIFLQAVSAGLLLA
                     SSYGETLHSVGARVTYGASMLYVLAAVLAWKPGGGSPRPIWHASGFLVLASAQVVLGIA
                     HVPSVHLPLGVLMFGLSVLALARTVASTRSRHISAPAHEPGVRG"
     gene            847..1698
                     /locus_tag="KGS77_RS02270"
                     /old_locus_tag="KGS77_02215"
     CDS             847..1698
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019891511.1"
                     /locus_tag="KGS77_RS02270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_02215"
                     /product="YwqG family protein"
                     /protein_id="WP_242578436.1"
                     /transl_table=11
                     /translation="MTQNTVDALHALAREHLPAEIAERWTGLLRPGLGLVNAGDSESDT
                     VVGRLGGQPELPAYEQWPVWEGHGPLSFIASLDCAALPSAALDIPLPTEGTLAFFYFDG
                     QLDDGEALVLPDDPDSWAGARVLYVPSGVPLVERAAPAGIEPYPEIRLAAHVETTAPYL
                     WHPVVDREFAALPDDHPLWSDDFQEALWDLPGAGHRVGGHADPVQDDVESEVAWGILGS
                     PPSGDPRIREEALRWVLLAQFDSDDDAGMMWGDCGTLYWLMRPEDLAARRFDRAKFTWQ
                     CG"
     gene            complement(1895..2569)
                     /locus_tag="KGS77_RS02275"
                     /old_locus_tag="KGS77_02220"
     CDS             complement(1895..2569)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003981451.1"
                     /locus_tag="KGS77_RS02275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_02220"
                     /product="hypothetical protein"
                     /protein_id="WP_242578437.1"
                     /transl_table=11
                     /translation="MTIGEAFWSGLKEWVRPPDRHAVLREAKARAAALLPPGEVVVDGH
                     TVEPGERVPEPPKPYRVDAFGIRPTVGDRVFNGVNRIDLVLDRLNPFNAAMDAWDRRNE
                     DRSAQWYGGWPSAAGRLAAALRPRTEEKYLSVLLLTGTEMHVIRVQLSHDRKKVAGAVE
                     HAYAVARQDITWLRDRKDVRHGTHEIGFADGSWVTVFFPLGRWGTLVEAFPHRLRHTDP
                     IP"
     gene            complement(2566..3522)
                     /locus_tag="KGS77_RS02280"
                     /old_locus_tag="KGS77_02225"
     CDS             complement(2566..3522)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003981450.1"
                     /locus_tag="KGS77_RS02280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_02225"
                     /product="hypothetical protein"
                     /protein_id="WP_242578438.1"
                     /transl_table=11
                     /translation="MGAGQAAGSALDRINYVLDWSNPLAATLDEVIKGVLKQFGVDDWF
                     DWVTGDSEKLEQTAEEWRSAARDLRGVVEDLVAERKSVQRVWESEAAEGFSKTMTDFEQ
                     ALLGEADDMDTVAELLEMAGAACAIAEQAMLDLLVEIVESVIAAAATTAILSLLTAGAA
                     AAIGPLAGAANVAYRAAKATRIAARLADKLADLARRTQALAKLSRLRRYLRGLDRAQIK
                     EWKGARGRIFESVRGGQRPEMTDIAQYLAWRQGKKLAKGIVGADPGGAVTGSIMENGPG
                     LAEEAYEYGKRPPSRSFDERMGQNPDQARKTVREAFG"
     gene            complement(3522..3833)
                     /locus_tag="KGS77_RS02285"
                     /old_locus_tag="KGS77_02230"
     CDS             complement(3522..3833)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003981449.1"
                     /locus_tag="KGS77_RS02285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_02230"
                     /product="hypothetical protein"
                     /protein_id="WP_242578439.1"
                     /transl_table=11
                     /translation="MSQGTNVQSDALRAYARAAESAADRIAKIRSRTAALDLSDGVFGQ
                     LPEANNLRSDYIDQKEQSGTDLEDSAEMLRSIADAVRGSADNYDANEEDTARGFKGGA"
     misc_feature    complement(4006..4021)
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 22.56"
                     /tool="antismash"
     gene            4118..4720
                     /locus_tag="KGS77_RS02290"
                     /old_locus_tag="KGS77_02235"
     CDS             4118..4720
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_function="GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1032: RNA
                     polymerase, sigma-24 subunit, ECF subfamily"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015661359.1"
                     /locus_tag="KGS77_RS02290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_02235"
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="WP_242578440.1"
                     /transl_table=11
                     /translation="MAAREARPEDVPQESDAGSTEDRFEIQARLLAADEQAFRTVYEQY
                     APLVRAVAARVTRDHCAVEDVVQVVFSQLWERPLSFDPARGSLRTWCAVAAHHRAVDWL
                     RRERKHRKPSPPDDGPAHRDETADTVIGGLLTLRVRSAVAQLPDTLRVPLLLAYYGGRT
                     YRQVATELGIPEGTAKSRLRTALRALAKELAEEGVEP"
     gene            4717..5889
                     /locus_tag="KGS77_RS02295"
                     /old_locus_tag="KGS77_02240"
     CDS             4717..5889
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /locus_tag="KGS77_RS02295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_02240"
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="WP_242578441.1"
                     /transl_table=11
                     /translation="MSEDGLGPATPEGHARARTLLAAWVLHACSPTETAAVTTHLRACE
                     QCTAEVRTLASAVAELAGAEPADVGMTGVRPAGPQSAEGQSAEAQSAGAERRNTGAPAG
                     PDSETYRRTAAGSFARRPPVPRELPAYVRPYAAQAATLDAVLRELTPDEWRLETVEGWS
                     VAQLVAHLAATDGLLTEETGGEVTGPVPVVRGEGVAMRTRTYTSWAADVPPGAVHTAWR
                     TQSEALRSALTHDGPGPGHHLLTLGGGGLPLPVADHAVGRAFETWVHTRDIGLRTGHRL
                     PPPTRASLTAIADLGVRLLPLALRARGTPLGDRVLRVELTGPGGGTWLLEDSGPATGRP
                     DAGFTLDTLEFCLLAGGRRTPAHISAHTHLTGDHSLARSALAAAPTFSGP"
     gene            6241..>7113
                     /locus_tag="KGS77_RS02300"
                     /old_locus_tag="KGS77_02245"
                     /pseudo=""
     CDS             6241..>7113
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1184: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014043619.1"
                     /locus_tag="KGS77_RS02300"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_02245"
                     /product="MFS transporter"
                     /pseudo=""
                     /transl_table=11
                     /translation="MSATTSASVDGAIPSARPPLKGWPAVVSVMLGIFAIVTTEILPIG
                     LLTSIGSDFTVSDSVAGLMMTMPGILAAISAPLITVATARVGRRLMLCVFMLLLALANL
                     LAAAASSYWLVLVSRVVVGVTIGGFWSIGSGIAERLVPPGSVGKATAVIFSAVPLGSVL
                     GVPVGTFIGEPAGWRTAFVVLGALAMGVLALLLGSMPPLSPVQATRLSVLRGMLRGTDT
                     RFALLLTFLAVLAHFGTYTYVTPFLEQVAHVGEGRITGVLLVYGAAGILGNFLGGARVA
                     TYPRTVFGLA"
     gene            complement(7165..7701)
                     /locus_tag="KGS77_RS02305"
                     /old_locus_tag="KGS77_02250"
     CDS             complement(7165..7701)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007385084.1"
                     /locus_tag="KGS77_RS02305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_02250"
                     /product="SUKH-4 family immunity protein"
                     /protein_id="WP_242578442.1"
                     /transl_table=11
                     /translation="MFSPNELITFPAEELTDIAHEQSVDFLRQVGIPFRDNPWFDLPDG
                     SPGCLKKLGECYKDLGIRWTNLPEEAGNWVVFGMIPYDDLALDVVTGAVYCLPDDRSEI
                     YLFNKDLPSFVHFLYLLEKGKPDYDADRDEEQEDWEEAARHIEAQMREVDPKALEFSDS
                     RWHDVLEYIVEPEIP"
     misc_feature    complement(7695..7709)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 20.59"
                     /tool="antismash"
     misc_feature    7696..7707
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: weak, score: 15.72"
                     /tool="antismash"
     gene            7919..8113
                     /locus_tag="KGS77_RS02310"
                     /old_locus_tag="KGS77_02255"
     CDS             7919..8113
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003986972.1"
                     /locus_tag="KGS77_RS02310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_02255"
                     /product="hypothetical protein"
                     /protein_id="WP_242578443.1"
                     /transl_table=11
                     /translation="MSAVREHLRAPHLSLPRQLPAGSARSLPMLDAVVEVLRAAGEDVH
                     VVYAAHGDVFKVVPRQEAD"
     gene            8238..8837
                     /locus_tag="KGS77_RS02315"
                     /old_locus_tag="KGS77_02260"
     CDS             8238..8837
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF013750.4"
                     /locus_tag="KGS77_RS02315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_02260"
                     /product="carbohydrate-binding module family 14 protein"
                     /protein_id="WP_242578444.1"
                     /transl_table=11
                     /translation="MKTVLLRSAAAFVAALALLLTGSAGSLAASAAPAAPSAPADTVYG
                     CPASGVRYLPYPDDAHKVVYCEAGYAYVTTCPADLIWNIRYEACDIPSDAHKYATSLAT
                     GTAHLVLAPLQVKGLNARLTWRDGRPLGGATLTFSTTTGTALCTARTDRNGWTSCDSAP
                     GLNVPVDTLRAGYQVTYKGIVTLGASQARGAVSTTS"
     misc_feature    complement(8916..8932)
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 21.05"
                     /tool="antismash"
     gene            9270..9419
                     /locus_tag="KGS77_RS02320"
                     /old_locus_tag="KGS77_02265"
     CDS             9270..9419
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF033521.1"
                     /locus_tag="KGS77_RS02320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_02265"
                     /product="keywimysin-related RiPP"
                     /protein_id="WP_242578445.1"
                     /transl_table=11
                     /translation="MKNDERVEDRELQVYEAPEMFEVGSFQEDTGYIGRKKTEPIAQIP
                     RSFW"
     gene            9591..10040
                     /locus_tag="KGS77_RS02325"
                     /old_locus_tag="KGS77_02270"
     CDS             9591..10040
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="KGS77_RS02325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="KGS77_02270"
                     /product="hypothetical protein"
                     /protein_id="WP_242578446.1"
                     /transl_table=11
                     /translation="MLTHRSGRPWVAGRLADGRIRCAEHGDTRLAVIGQCAATPAELAS
                     AAARITDVAQLDALGTAWAGSFHLIASVGGRLRVQGSASGLRRVFHGLFGGVVIAADRA
                     DVLASLLDAPLDRSALALRLLGDVPHPLGDRPLWKNVTAVPRARV"
     gene            10001..11461
                     /locus_tag="KGS77_RS02330"
                     /old_locus_tag="KGS77_02275"
     CDS             10001..11461
                     /GO_function="GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA]"
                     /GO_process="GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: Asn_synthase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1177: asparagine synthase (glutamine-hydrolyzing)"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /locus_tag="KGS77_RS02330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_02275"
                     /product="asparagine synthase-related protein"
                     /protein_id="WP_242578447.1"
                     /sec_met_domain="Asn_synthase (E-value: 1.1e-37, bitscore:
                     129.2, seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEKRHSSAPGSRLTLSPDGRQHTVKRWWQPPEPVLSLEAGARALR
                     QALEDAVRVRTSGPEPISTDLSGGLDSTTLTALAVQDRASLTAFTMENDDAADDDAHWA
                     KIAAAHLPHLDHVLFPGHELPGFYEGLTGLDDVPDEPSIALLSAPRLRAGRDRATAHGS
                     RLHLDGFGGDQLLTGHPAHYHDLLRSRPLLALRRLRTQHLLTGTGHPAGSSVRTLLDGG
                     SYRRWFTAGTEALAGGRPRRPRNPLFDWGDALRLPPWLTPEAVGLVTDALREAARAAQP
                     LAPTRGRHSDLLAVQDAGRMIRQFHQLVEGAVGTTAGGPVGRTAGRTDGGREFTPASPF
                     LDDRVADACLSVRPEERVTPWEFKPLIKAAMADVLPGELLRRRTKGDGSTIAAEGFERS
                     RRELAELWENSRLAELGLIDPAPLRELFRRPYTARLHDGAMPVTLGCELWARAAVPAGP
                     AASAGSAGFTGSAGATGSAAPVAGGARA"
     aSDomain        11506..11655
                     /aSDomain="Lasso_Fused_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="RRE-containing fused peptidase in a lasso
                     peptide cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_KGS77_RS02335_Lasso_Fused_RRE.1"
                     /evalue="2.10E-09"
                     /identifier="RREFam003.1"
                     /label="KGS77_RS02335"
                     /locus_tag="KGS77_RS02335"
                     /protein_end="50"
                     /protein_start="0"
                     /score="28.3"
                     /tool="antismash"
                     /translation="MTLKLRADVSTADTEYGTVLLDGSTGRYWQLNATASLALRTLLEG
                     GTAEE"
     aSDomain        11506..11757
                     /aSDomain="Stand_Alone_Lasso_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="Stand-alone RRE protein in a lasso peptide
                     cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_KGS77_RS02335_Stand_Alone_Lasso_RRE.1
                     "
                     /evalue="5.90E-26"
                     /identifier="RREFam002.1"
                     /label="KGS77_RS02335"
                     /locus_tag="KGS77_RS02335"
                     /protein_end="84"
                     /protein_start="0"
                     /score="81.3"
                     /tool="antismash"
                     /translation="MTLKLRADVSTADTEYGTVLLDGSTGRYWQLNATASLALRTLLEG
                     GTAEEAARRLTTGYDVEESRARADVEKLLAGLLAAKVAE"
     gene            11506..11763
                     /locus_tag="KGS77_RS02335"
                     /old_locus_tag="KGS77_02280"
     CDS             11506..11763
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: Stand_Alone_Lasso_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Lasso_Fused_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF05402"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015656042.1"
                     /locus_tag="KGS77_RS02335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_02280"
                     /product="lasso peptide biosynthesis PqqD family chaperone"
                     /protein_id="WP_242578448.1"
                     /sec_met_domain="Stand_Alone_Lasso_RRE (E-value: 1.4e-23,
                     bitscore: 81.3, seeds: 25, tool: rule-based-clusters)"
                     /sec_met_domain="Lasso_Fused_RRE (E-value: 5.2e-07,
                     bitscore: 28.3, seeds: 20, tool: rule-based-clusters)"
                     /sec_met_domain="PF05402 (E-value: 2.2e-12, bitscore: 45.7,
                     seeds: 461, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTLKLRADVSTADTEYGTVLLDGSTGRYWQLNATASLALRTLLEG
                     GTAEEAARRLTTGYDVEESRARADVEKLLAGLLAAKVAEA"
     gene            11760..12236
                     /locus_tag="KGS77_RS02340"
                     /old_locus_tag="KGS77_02285"
     CDS             11760..12236
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: PF13471"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004949361.1"
                     /locus_tag="KGS77_RS02340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_02285"
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="WP_242578449.1"
                     /sec_met_domain="PF13471 (E-value: 3.9e-27, bitscore: 93.2,
                     seeds: 84, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTVVPHRADLRLTPGRRLLTALAVYAARRLARKPPAHIRTVLTR
                     LRKGARPATYAEAQTAREAVLAVSLRCAGPEGCLPRSLATVLLCRAGGTWPAWVVGVRA
                     RPPFGAHAWVEAEGRMVDEEAGPEYFRALFTVPAARSAGAEQNLGKTSGTSKQQ"
     gene            12318..12545
                     /locus_tag="KGS77_RS02345"
                     /old_locus_tag="KGS77_02290"
     CDS             12318..12545
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608014.1"
                     /locus_tag="KGS77_RS02345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_02290"
                     /product="hypothetical protein"
                     /protein_id="WP_242578450.1"
                     /transl_table=11
                     /translation="MAASPPVIVHPPTPSGGRRVTLRGRFAGLAHGRGDVAEFLRRAGL
                     APDSEDIDLDDPELIEWRGGDLDTWPEPQG"
     gene            12742..12924
                     /locus_tag="KGS77_RS02350"
                     /old_locus_tag="KGS77_02295"
     CDS             12742..12924
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018514676.1"
                     /locus_tag="KGS77_RS02350"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_02295"
                     /product="hypothetical protein"
                     /protein_id="WP_242578451.1"
                     /transl_table=11
                     /translation="MSPTPPGCAGTSLRAAEANEAIRQFVRARRGHVWTAEDRSRYRQL
                     LETWLVAVHGSVSGA"
     gene            13265..13861
                     /locus_tag="KGS77_RS02355"
                     /old_locus_tag="KGS77_02300"
     CDS             13265..13861
                     /EC_number="2.1.1.63"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019984988.1"
                     /locus_tag="KGS77_RS02355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_02300"
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="WP_242578452.1"
                     /transl_table=11
                     /translation="MALHTTAPATAFPPTPPSTVTATATATATAAGSSTLYASIGSPLG
                     ELLLVGEESPTARGGIALASLSMSGQKGAAIVQDGWRRAPEAFAEIAEQLAAYFDGART
                     HFDIDRVIGRGTAFQQRVWDALETIPYGTTTSYGALAEAAGISRAAVRALGTAIGANPL
                     LIVRPCHRVIGANGTLTGYAGGLERKRRLLDLEQA"
     gene            13858..14661
                     /locus_tag="KGS77_RS02360"
                     /old_locus_tag="KGS77_02305"
     CDS             13858..14661
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020139894.1"
                     /locus_tag="KGS77_RS02360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_02305"
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="WP_242578453.1"
                     /transl_table=11
                     /translation="MTTRVPSAVDSADRPGRPAWTDDPGGTDEPGGTDGTDRLGRADWP
                     SLTDELNAYGCALTPRLLSTRECREIAELYDEAERFRATVDMARHRFGSGQYRYFNRPY
                     PEPVQRLREAFYPRLLPIARDWAEKLRRPAPWPDTLEEWLAMCHRADQARSTAILLRYE
                     EGDWNALHRDLYGDLVFPLQVVIGLDEPGTDYTGGEFLLVEQRPRAQSRGTATVLRQGH
                     GLIFTTRDRPVRSTRGWSTGPVRHGVSTVRSGRRHALGLVFHDAA"
     gene            14920..15588
                     /locus_tag="KGS77_RS02365"
                     /old_locus_tag="KGS77_02310"
     CDS             14920..15588
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007445943.1"
                     /locus_tag="KGS77_RS02365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_02310"
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="WP_347404425.1"
                     /transl_table=11
                     /translation="MYDHGSPSRPRTSPAPGAVHVPAWLTLEQQRELVTACRDWARGPV
                     PIRHTKLPRGGVMSVQTVCIGWHWQPYAYTRTADDVNGARVAEFPDWMVELGRRALVDA
                     YGEESAGDGYTPDTALINFYDGDGRSKLGMHQDKEERSDAPVVSLSIGDTCVFRFGNTE
                     TRTQPYTDIELASGDLFVFGGPSRWAYHGVPKTYPGTGDPATGLARGRLNITMRVTGLP
                     "
     gene            15679..15897
                     /locus_tag="KGS77_RS02370"
                     /old_locus_tag="KGS77_02315"
     CDS             15679..15897
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016574711.1"
                     /locus_tag="KGS77_RS02370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_02315"
                     /product="DUF2277 domain-containing protein"
                     /protein_id="WP_242578455.1"
                     /transl_table=11
                     /translation="MCRSIKTLRPPATPEVTDEDIRAAALQYVRKVSGFRAPATHNREV
                     FEHAVDAVAAATQDLLAGLRVRGSSAH"
     gene            complement(15974..16836)
                     /locus_tag="KGS77_RS02375"
                     /old_locus_tag="KGS77_02320"
                     /pseudo=""
     CDS             complement(15974..16836)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010037575.1"
                     /locus_tag="KGS77_RS02375"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_02320"
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MAPGTTGTGADADAGPSSAPASSPARAAGPGLRVMSFNVRYASDV
                     GPHSWPERRPVMRALLRAAAPHLLGTQEGCTASSARWQRTSVTTTPGWERGGPGAAATS
                     SWRSSMTSADWSRSSTTTSGCPTARI"
     misc_feature    16919..16933
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.7"
                     /tool="antismash"
     gene            complement(17016..17632)
                     /locus_tag="KGS77_RS02380"
                     /old_locus_tag="KGS77_02325"
                     /pseudo=""
     CDS             complement(17016..17632)
                     /GO_function="GO:0010181 - FMN binding [Evidence IEA]"
                     /GO_function="GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014181370.1"
                     /locus_tag="KGS77_RS02380"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_02325"
                     /product="flavin reductase family protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MRIDFDPAAVSRNDFYRLLTATVVPRPIAWVSTIDRDGVPNLAPH
                     SFFTISCVAPPIVQFTAVGRKDSLRNVEETGQFVVNLADERHFRQINDTAVDFPRGVNE
                     FERAGIDSEPSLRVKPPRVAASPVAFECELHSTLLLGDSTVVFGRVVHAAVSEEVMVDG
                     HPEITKLRPLSRLGKDEWGRSGTYGSCPVSGSPTGRGQRRPS"
     misc_feature    17630..17645
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 17.19"
                     /tool="antismash"
     gene            18020..18442
                     /locus_tag="KGS77_RS02385"
                     /old_locus_tag="KGS77_02330"
     CDS             18020..18442
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="KGS77_RS02385"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="KGS77_02330"
                     /product="hypothetical protein"
                     /protein_id="WP_242578456.1"
                     /transl_table=11
                     /translation="MCPPLQGHIDRATEFAPEDEERQRQDFLAGTVPAAGAGIAVPGDG
                     PGGPLDALFMIFVHEDQRTFGPAPQLKEAFALLDRYGAVGLHDQLSVGTGWAGMGGRDP
                     LVKLKLQFHDALSGSTGIVLAAHRNATGGGMSSAAA"
     gene            18439..18594
                     /locus_tag="KGS77_RS02390"
                     /old_locus_tag="KGS77_02335"
     CDS             18439..18594
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="KGS77_RS02390"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="KGS77_02335"
                     /product="hypothetical protein"
                     /protein_id="WP_242578457.1"
                     /transl_table=11
                     /translation="MIALTSLERMDRATSRPGATFADALEVSILLEVGTSPVLERMIDI
                     YDWPRP"
     gene            complement(18671..19249)
                     /locus_tag="KGS77_RS02395"
                     /old_locus_tag="KGS77_02340"
     CDS             complement(18671..19249)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003971372.1"
                     /locus_tag="KGS77_RS02395"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_02340"
                     /product="restriction endonuclease"
                     /protein_id="WP_242578458.1"
                     /transl_table=11
                     /translation="MLLTQWLLAHLWVLALVGVLAALTAGGYVHRARQRAAWDRVRRQA
                     LRLRPAQLDALDHRDFETAVRDLMRRDGCRAERLGGAGDDACDVKATDPLGRVWAVQCK
                     HRRDGLRGQAVGTGVLQQVNGTAKPVHRADVAVVVTNGRFSSKAIPWGHRHGIHLVDRA
                     VLVEWAGGSRPLWEILDRLPPPRRSVTFN"
     misc_feature    complement(19217..19219)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            19660..20154
                     /locus_tag="KGS77_RS02400"
                     /old_locus_tag="KGS77_02345"
     CDS             19660..20154
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006606503.1"
                     /locus_tag="KGS77_RS02400"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_02345"
                     /product="glutathione peroxidase"
                     /protein_id="WP_242578459.1"
                     /transl_table=11
                     /translation="MSADLYGIPLRTLAGEPVSLADYRGRALLVVNVASKCGLTPQYAG
                     LERLQERFGARGLSVLGFPCNQFAGQEPGTAEEISTFCSTTYGVSFPLFEKTEVNGPDR
                     HPLYAELTRAQDAEGESGDVQWNFEKFLVNGQGEVVGRFRPRVEPESEEVVRAVEAVLP
                     G"
     gene            complement(20269..20664)
                     /locus_tag="KGS77_RS02405"
                     /old_locus_tag="KGS77_02350"
     CDS             complement(20269..20664)
                     /GO_function="GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /locus_tag="KGS77_RS02405"
                     /note="This anti-anti-sigma factor, or anti-sigma factor
                     antagonist, belongs to a family that includes characterized
                     members SpoIIAA, RsbV, RsfA, and RsfB; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /old_locus_tag="KGS77_02350"
                     /product="STAS domain-containing protein"
                     /protein_id="WP_242578460.1"
                     /transl_table=11
                     /translation="MLDEGRTFGSRVRPTSFGVVLELSGELDIFATTTLSQRIDELTNV
                     RRPDLVLDMSEVTFIDCGGLSLLCRARQRIAARDGRLRLAGVAASPSTQRLLRLTGMTH
                     CFDLCDPPPSAIPRTAATTNSDNSVIA"
     misc_feature    20866..20884
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 19.44"
                     /tool="antismash"
     misc_feature    21000..21014
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 18.37"
                     /tool="antismash"
     misc_feature    complement(21629..21642)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 19.57"
                     /tool="antismash"
     misc_feature    complement(21810..21822)
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 19.11"
                     /tool="antismash"
     misc_feature    complement(21983..21997)
                     /note="TFBS match to NrdR, Represses ribonucleotide
                     reductase encoding genes, confidence: weak, score: 21.32"
                     /tool="antismash"
ORIGIN
        1 cggctggtca ccgtcgtcag ccaggcgccg ggggacagga tctccgcccg tcggcttcgc
       61 ggcagcccgt accagcgtgc gtaggcttcc tgtacggcgt cctcggcctc ggtcaccgaa
      121 ccgagcaacc ggtacgcgac gttgatcagt tggcgtcgct cgccggctac ctcgtccgcg
      181 ccggccccca cactccccat gcttccggcc gcccctcgcc ttacctttcg cgggcccgtg
      241 tcgtcgggct ggtgagacct tatcgatctg ctgcccgagg gcggaaaagg ggaccgtgac
      301 atggccactg agtcgacggt gacggcaagg gcgcgggcga acgtaccgcg cggctccacg
      361 ctcttgcagg tcgcgatcgg gttgcagacc ctgaccatct tccttcaggc ggtctccgcc
      421 ggactgctgc tggcctcgtc ctacggggag acgctgcaca gcgtgggagc ccgtgtgacg
      481 tacggggcgt cgatgctgta cgtgctcgcg gcggtgctgg cgtggaagcc gggcggcggc
      541 tcgccccggc ccatctggca tgcgtccggt ttcctcgtac tggcctcggc gcaggtcgtg
      601 ctcggcatcg cccacgtccc ttcggtccac ctccccctgg gtgtcttgat gttcggcctg
      661 agtgtgctgg cactggcccg gaccgttgct tccacgcgta gccggcacat cagcgcacca
      721 gcacatgagc ccggcgtccg cgggtaaccg tgcggcacag cgtgagcacg gagggccggc
      781 cggtgcgggc ggtccggccc atgccccggg cggcccgttg tcagtggtcc cgtgcatgat
      841 cgacccatga cacagaacac cgtcgatgcg ctgcacgcgc tcgcccgcga acatctgccc
      901 gccgagatcg ccgagcggtg gaccggactg ctacggccgg gtctgggcct ggtgaacgcc
      961 ggggactcgg aatcggacac ggtcgtcggc cggctcggcg gtcagcccga gctgcccgcg
     1021 tacgagcagt ggccggtgtg ggagggacac ggtccgctgt ccttcatcgc ttccctggac
     1081 tgcgccgcgc tcccttccgc cgccctcgac atccccttgc cgacggaggg caccctggcc
     1141 ttcttctact tcgacgggca gttggacgac ggtgaggcat tggtcttgcc cgatgacccc
     1201 gacagttggg cgggggcgcg tgtactgtac gtgccctccg gcgtccccct ggtggagcgg
     1261 gcggcgcctg cgggtatcga gccctacccg gagatacggc tggcagccca tgtggagacc
     1321 acggccccct acctctggca tccggtggtc gaccgggagt tcgcggcgtt gcccgacgat
     1381 catccgctgt ggagcgatga cttccaggag gccctttggg acctcccggg cgccggacac
     1441 cgcgtaggag gccacgccga cccggtgcag gacgatgtgg agtccgaggt cgcctggggc
     1501 atcctgggca gtccgccgag cggcgatccg cgtatccgtg aggaggcgct gcgctgggtg
     1561 ctgctcgccc agttcgactc ggacgacgac gccggcatga tgtggggcga ctgcggcacc
     1621 ctgtactggc tcatgcgccc cgaggacctg gccgcacgcc gcttcgaccg ggcgaagttc
     1681 acctggcagt gcggctgagg ccggtcgggg tgtacctgac cgggaagcct ctggttgcgg
     1741 gaccggagtc tcgcgaagcg agctctgacg agcgcttatg agctagccgg gtgcccagtc
     1801 agggaggcgg atggccagga aaccggacgt tccgggcgtc ggacgttctg ggcgtcggac
     1861 gttccgggta tcggacgctc agcgaaccgg acgctcaggg aatcgggtcg gtgtgccgca
     1921 gtcggtgcgg gaacgcctcc accagcgtgc cccagcggcc gagagggaag aacaccgtca
     1981 cccacgaccc gtcggcgaag ccgatctcgt gcgtgccgtg ccgtacgtcc ttgcggtccc
     2041 gcagccaggt gatgtcctgg cgggcgacgg cgtaggcgtg ctcgacggcg ccggccacct
     2101 tcttgcggtc gtgggagagc tggacgcgta tcacatgcat ttcggttccg gtgagcagca
     2161 gcaccgacag gtacttctcc tcggtgcgcg ggcgcagggc cgccgccagc cgcccggccg
     2221 cgctcggcca gccgccgtac cactgcgcgc tgcggtcctc gttccgccgg tcccaggcgt
     2281 ccatggccgc gttgaagggg ttgagccggt ccagcaccag atcgatacgg ttcacaccgt
     2341 tgaacacccg gtcgccgacc gtgggccgta tgccgaaggc gtccacccgg tacggtttcg
     2401 gcggctccgg tacccgttcg cccggctcga cggtgtgccc gtccaccacc acctcgccgg
     2461 gtggcagcag cgccgccgcc cgcgccttgg cctcgcgcag gaccgcgtgc cggtcgggcg
     2521 gcctcaccca ctccttgaga cccgaccaga acgcttcacc gattgtcacc cgaacgcctc
     2581 ccggacagtc ttcctggcct ggtcggggtt ctgccccata cgctcgtcga agctccgcga
     2641 cggcgggcgc ttgccgtatt cgtacgcctc ctccgcgagc ccggggccgt tctccatgat
     2701 cgagccggtc acggcgccgc ccggatcggc accgactatc cccttggcca gcttcttgcc
     2761 ctgcctccac gccagatact gagcgatatc ggtcatctcg ggccgctgac cgccccgcac
     2821 gctctcgaag atccgccccc tcgcgccctt ccactccttg atctgcgcgc ggtccagtcc
     2881 gcgcaagtag cggcgcagcc gggagagttt ggccagggcc tgggtccggc gggcgaggtc
     2941 ggcgagcttg tcggccagcc tcgccgcgat acgggtcgcc ttggccgccc ggtacgccac
     3001 gttcgccgcg cccgccagcg gaccgatcgc cgccgccgcg cccgccgtca gcagcgagag
     3061 gatcgccgtg gtcgcggcgg cggcgatcac cgactcgacg atctcgacga gcaggtccag
     3121 catcgcctgc tcggcgatgg cgcaggccgc tccggccatc tccagcagct cggcgacggt
     3181 gtccatgtcg tcggcctcgc cgagcagtgc ctgctcgaag tcggtcatgg tcttgctgaa
     3241 gccttccgcg gcctcgctct cccacacccg ctggacggac ttgcgctcgg cgaccaggtc
     3301 ctcgacgacg ccccgcaggt cacgggccgc gcttcgccac tcctcggccg tctgctccag
     3361 cttctcgctg tcgccggtga cccagtcgaa ccagtcgtcg acgccgaact gcttgaggac
     3421 gcccttgatg acctcgtcga gggtggccgc gaggggattg ctccagtcca ggacgtaatt
     3481 gatgcggtcc agcgcactgc ccgcggcctg tccggcaccc atcaggcacc gcccttgaag
     3541 ccgcgcgccg tgtcctcttc gttggcgtcg tagttgtccg cggacccgcg tacggcgtcg
     3601 gcgatggagc gcagcatctc cgcgctgtcc tccaggtccg tacccgactg ctccttctgg
     3661 tcgatgtagt cggagcgcag attgttggcc tccggcaact gcccgaagac gccgtccgac
     3721 agatccagtg cggccgtacg actgcggatc ttcgcgatcc ggtcggccgc gctttcggcg
     3781 gcacgcgcgt acgcacgcag cgcgtcgctc tgaacgttgg tgccctgact catgagaccc
     3841 ccgttgccat tcctttgcga aatcatgaca cacgatgcac cggcgtacgt caatggaatc
     3901 cctgcacagc aggggctgtt gccccttttt ggccgatgtg gaagcagcgg gcacagccgg
     3961 caccctcgta cgtcagaacg tcagtggcac cgtgggtgac cgggaggatc gcgatcagcg
     4021 tggttcgtcg acatgttgac cggccggacg ggcgacggcg tcggacggtg ccggcccagg
     4081 cggtcccgga gccccgctct tacgggatac tgcccctatg gccgcccgtg aggcgcggcc
     4141 cgaggacgtg ccgcaggagt ccgacgccgg aagcaccgag gaccggttcg agatccaggc
     4201 ccgtctgctg gccgccgacg agcaagcgtt ccgcacggtg tacgagcagt acgcgccgct
     4261 ggtacgggcc gtcgcggccc gggtcacccg ggaccactgc gccgtcgagg acgtcgtcca
     4321 agtggtcttc tcccaactat gggaacgccc gctgtccttc gaccccgcgc gcggttcgct
     4381 gcggacatgg tgtgcggtgg ccgcccacca ccgggcggtc gactggctgc gcagggagcg
     4441 gaagcaccgt aaacccagcc ccccggacga cgggcccgcg caccgggacg agacggccga
     4501 cacagtgatc ggagggctgc tgacactgcg ggtcaggtcc gcggtcgccc aactcccgga
     4561 caccttgcgc gttccgctgc tgctggccta ctacggagga cggacctacc ggcaggtggc
     4621 caccgaactc ggcattcccg aaggtacggc caagtcgcgg ctgcgaacgg ccctgcgcgc
     4681 gctggccaaa gaactcgcgg aagaaggggt tgagccatga gcgaggacgg cttgggcccc
     4741 gccacgcccg aggggcacgc acgggcccgg acgctgctgg cggcgtgggt gctgcatgcc
     4801 tgctccccta cggagacggc tgccgtgacg acgcatctac gggcgtgcga acaatgcacg
     4861 gcggaggtac ggacgttggc ctccgccgtc gcggaactgg ccggagcgga gccggccgac
     4921 gtcggcatga cgggagtgcg accagccgga ccgcaatcgg ccgaaggaca atcggccgaa
     4981 gcgcaatcgg ccggcgccga acgtcgcaac accggagccc ccgccggccc ggactccgag
     5041 acgtaccggc gaacggccgc cggctccttc gcgcgccggc cgcccgtgcc gcgcgagctg
     5101 cccgcgtacg tacggcccta tgcggcgcaa gcggccaccc tggacgccgt actgcgcgaa
     5161 ctgaccccgg acgaatggcg gcttgagacc gtcgagggct ggagcgtcgc ccagttggtc
     5221 gcgcacctcg cggcgacgga cggcctgctg accgaagaga ccggcggcga ggtgaccgga
     5281 ccggtaccgg tcgtacgcgg ggaaggcgtc gccatgcgca cccgcactta cacttcctgg
     5341 gccgccgacg tgccccccgg cgccgtacac accgcctggc gcacccagag cgaagcgctg
     5401 cgctcagccc tgacgcatga cggcccgggc cccggccatc acctcctgac cctagggggc
     5461 ggcggcctgc cgctgccggt ggccgaccat gccgtcgggc gggcgttcga gacctgggtg
     5521 cacacccggg acatcggcct gcgcaccgga caccggctgc cgccacccac ccgcgcatcc
     5581 ctcaccgcca tcgccgacct cggcgtccgc ctgctgccgc tcgccctacg ggcccgggga
     5641 acgccgctcg gcgaccgcgt actgcgcgtg gagctgaccg gcccgggcgg cggcacctgg
     5701 ctgctggagg actccggccc cgccaccggc cggccggacg ccggattcac cctcgacacc
     5761 ctggagttct gcctcctggc cggaggccgc cgcacccccg ctcacatcag cgcccacacc
     5821 cacctgaccg gcgatcattc cctggcccgg tccgccctcg ccgccgcccc caccttctcc
     5881 ggcccgtaac tccaccgttt ttctgccgcc ggtctctcgc cgtacgcggc catcgagccg
     5941 ccttcgcgag ccgcgccctg ttcgcgacgg acttctgtcc ccggcttcgg gcacgtggcc
     6001 ttgcccgaag gcgaaggccg accgtgtcga agacagccag gccgacgaac cgcgcgcttg
     6061 gcccggaccg ggagcggatg aggagaaccc gcaaggaaac cgcgttgccg cgctgtccgc
     6121 gtggcgggaa tgcgacagca cccgtagagc gcgaggatgc cgtcgcagac ctgccagccg
     6181 gccggggctg gtgcctgatg agctgatcgc ggaatgactg ttcacgacag gacggaattc
     6241 atgtccgcaa ccaccagcgc atcggtcgac ggtgcgattc catcggctcg gcctccgctg
     6301 aaaggctggc cggccgtggt atccgtgatg ctggggatct tcgccatcgt cacgaccgag
     6361 atcctgccca tcggcctact gacctcgatc ggctccgatt tcaccgtctc ggacagtgtg
     6421 gccggactga tgatgacgat gccgggaatt ctcgcggcca tatccgctcc cctgatcacc
     6481 gtggccacgg cccgcgtcgg ccgtcgcctg atgctgtgtg tcttcatgct cctgctggcc
     6541 ctggcgaatt tgctggccgc ggcggcgtcc agctactggc tcgtgctggt ttccagggtc
     6601 gtggtgggag tcaccatcgg cggattctgg tcgatcggga gcgggatcgc cgagcggttg
     6661 gtgccgccgg gttccgtcgg aaaggcaact gccgtgattt tctcagccgt tccgttgggc
     6721 tccgttctcg gggtacccgt cggcaccttc atcggggagc cggccgggtg gcgtacggca
     6781 ttcgtcgtcc tgggcgcgct ggcgatgggg gtgctggcct tgctgctggg cagcatgccc
     6841 ccgttgtctc ctgttcaagc cacccggctg agcgtcctga gaggcatgct gcgtggcacc
     6901 gacacccgct tcgcgctgct gctgacgttc ctggccgtgc tggctcactt cggaacctat
     6961 acctatgtaa cgccgttcct ggaacaggtc gcccacgtcg gggagggccg tatcaccgga
     7021 gttctgctgg tgtacggagc cgcaggcatc ctcggcaact tcttgggcgg tgctcgcgtg
     7081 gcaacgtatc cgcggaccgt cttcgggctg gcggcccact tagccgggac ccggcgggat
     7141 gaaagcggtc cgttctgagg ggcgtcacgg tatctcgggc tcgacgatgt actccagcac
     7201 gtcgtgccac cgcgagtcgc tgaattccag tgccttcggg tcgacctctc tcatctgggc
     7261 ctcgatgtgc cgcgccgctt cctcccagtc ctcctgttcc tcatcccggt cggcgtcgta
     7321 gtccggcttg cccttctcca gcaggtagag gaaatggacg aaggagggga ggtccttgtt
     7381 gaagaggtag atctcggacc tgtcgtcggg aaggcaatag accgctcccg tgacgacgtc
     7441 cagtgcgagg tcgtcatagg gaatcatgcc gaagaccacc cagtttcccg cttcttcggg
     7501 gaggttcgtc cagcggatcc ccaggtcctt gtagcattca ccgagtttct tgaggcatcc
     7561 gggagatccg tcgggcaggt cgaaccaggg gttgtcgcga aaagggatac ccacctggcg
     7621 caggaagtcc acgctctgtt cgtgtgcgat atctgtcagt tcttccgccg ggaaggtgat
     7681 gagttcgttt gggctgaaca cggtttccag cgttgcccgg tcgatggggt tcgtcatgtg
     7741 gggggcctgt tctttcgtac gtcgacgttc tgcgtgagat gaacgattcc ggtggcgaaa
     7801 cagttccgcg cgggacccgt gggccggtct cggggcggtg cctcctcagc agacgttgag
     7861 caggtgttca tcgccgcgtt tcaggatggg gggaccaact cgtgattgag gatgtgacat
     7921 gagcgcagtt cgtgagcatc tccgtgctcc tcatctgtcg ttgccccgcc aactgcccgc
     7981 gggcagcgcg cggtcgctgc cgatgctgga cgcggtggtg gaggtgttgc gcgccgccgg
     8041 agaggacgtg catgtggtgt acgcggcgca cggcgacgtg ttcaaggtcg taccgcggca
     8101 ggaggccgac tgacggccgg tggcgggaac gagcggtcgg gattcggacg gaacgcgcca
     8161 agtggtgtag gagtggcgcg cacggaaggc gttgtccgcg atgctcataa cctcgtcgac
     8221 ggaaggaact ccttctgatg aagaccgtgt tgctccgttc agccgcggcg ttcgtcgccg
     8281 cactcgctct ccttctcacc ggatccgcag ggtcgctcgc ggcgtccgcc gcccccgccg
     8341 caccgtccgc ccccgccgat acggtctacg gctgtcctgc ctcgggggtg cgctacctcc
     8401 cgtacccgga cgacgcccac aaggttgtgt actgcgaggc cggctacgca tatgtcacca
     8461 cttgtccggc ggacctgatc tggaacatcc gctacgaggc ctgtgacatc ccctccgacg
     8521 cgcacaagta cgccacgtcg ctggccaccg gcaccgcaca cctggtcctc gccccgctcc
     8581 aggtcaaggg gctcaacgcg cggctgacct ggcgcgacgg ccgtccgctg ggcggcgcca
     8641 cgctcacctt cagcacgacg accggtacgg cgctgtgcac ggcccgcacc gaccgcaacg
     8701 gctggacctc ctgcgactcc gcacccgggc tcaacgtccc ggtggacacc ctgcgggccg
     8761 ggtaccaggt cacgtacaag ggcatcgtga ccttgggcgc ctcgcaggcg aggggcgcgg
     8821 tcagcaccac ctcctgacat ctgcctgccg ccggcgaccg gtcagccggg atcggtccgg
     8881 cccggggcac gccgtacgca tatcggcgta cggcgtgccc cattgtcatg cagcagaagg
     8941 agcgacggaa cggcggaata gcgacatggc ggggcgtccg ggttgcggcc ggccccaact
     9001 taactgccat cgacgcgatc tgactgaccg tcaagcgaaa tgccgcattc cactacgccc
     9061 ccggattcgg tcctgaaagg tcttgatctt cgcgatcgac cgtggttact gtttggttgg
     9121 acgccaggga attttttcac ggtgacggaa ttccgcgctg tttctgccgg ctaccggcgg
     9181 agcagtgttt ccattccgtc tgaatttccc gtgcacagaa ttcgctgcgt ctcgtaattt
     9241 ccatgtcagg agacggaggt gaatgcagga tgaagaacga cgagcgcgtg gaggaccgcg
     9301 aactccaggt ctacgaggcc ccggagatgt tcgaggtcgg ctccttccag gaggacaccg
     9361 gctacatcgg ccgcaagaag accgagccca tcgcccagat ccctcggagc ttctggtaaa
     9421 tcgcgtccga tgtgcacagg tactgacgtg ccgacgcact gacgtgctga cgtgccgagt
     9481 ccggtgagga ggaagaaatg gtcggtcccg ggaagcaata tttcgtggtt ctgccggacc
     9541 acgaaaccgc ggccacggcc gccgaccggc ttccgccgga caatgttcgg atgctgacgc
     9601 accggtccgg ccggccctgg gtggccggcc ggctggccga cggccgcatc aggtgcgccg
     9661 agcacgggga cacccggctc gccgtcatcg gccagtgcgc ggcgacgccc gccgaactgg
     9721 cgtcggcagc cgcgcggatc accgacgtgg cccagctcga cgcgctgggc acggcctggg
     9781 ccggcagttt ccacctcatc gcctcggtcg gcggccggct gcgcgtccag ggcagcgcct
     9841 ccggactgcg ccgggtcttc cacggcctgt tcggcggggt ggtgatcgcc gcggaccggg
     9901 ccgatgtgct ggcctcgttg ctcgatgccc cgctggaccg ctcggcgctg gcgctgcgcc
     9961 tgctcggtga cgtaccgcat ccgctgggcg accggccact gtggaaaaac gtcacagcag
    10021 tgccccgggc tcgcgtctga cgctctcccc cgacgggcgg cagcacacgg tgaagcgctg
    10081 gtggcagccc ccggagccgg tgctgtcgct ggaggcgggg gcgcgggcgc tacggcaggc
    10141 gctggaggac gccgtacgcg tacggaccag cggccccgaa ccgatcagta cggacctgtc
    10201 cggtggcctg gactccacca ccctgaccgc gctggccgta caggaccgtg cctcgctgac
    10261 cgccttcacc atggagaacg acgacgcggc cgacgacgac gcccactggg ccaagatcgc
    10321 cgcggcccat ctgccgcacc tcgaccacgt cctgttcccg ggccatgaac tccccggctt
    10381 ctacgagggg ctgacgggcc tggacgacgt acccgacgag ccgtccatcg cgctgctgag
    10441 cgctccccgg ctgcgggccg gccgggaccg ggcgaccgcc cacggctcgc gtctgcatct
    10501 ggacggcttc gggggcgacc agctcctgac gggtcatccg gcgcactacc acgacctgct
    10561 gcgcagccgt ccgctgctcg cgctgcggcg gctgcgcacc cagcacctgc tcaccggtac
    10621 cggccaccct gccggcagtt ccgtacggac cttgctcgac ggtggttcct accgccgctg
    10681 gttcacggcc gggaccgagg cactggccgg cggccggccc cgccgcccgc gcaaccccct
    10741 cttcgactgg ggcgatgccc tgcgccttcc cccgtggctc acaccggagg ccgtcggcct
    10801 ggtcaccgat gccctgcggg aggcggcccg ggcggcacag ccgctcgcgc ccacccgggg
    10861 ccggcacagc gatctgctgg cggtccagga cgccgggcgc atgatccgcc agttccacca
    10921 gttggtggag ggggcagtgg ggacgactgc tggggggccg gtcgggagga cggctggaag
    10981 gacggacggg ggaagggagt tcacgccggc ttccccgttc ctcgacgatc gggtcgccga
    11041 cgcctgcctg tccgtacggc ccgaagaacg ggtgaccccc tgggagttca agccgctgat
    11101 caaagcggcc atggcggacg tcctgccggg cgagttgctg cgacggcgga ccaaggggga
    11161 cggctcgacg atcgcggccg agggcttcga acggagccgg cgggagctcg ccgagctgtg
    11221 ggagaactcc cggctggccg agctgggact gatcgacccg gcgccgctgc gcgagctgtt
    11281 ccgccgtccg tacaccgccc ggctccacga cggggcgatg ccggtgacgc tcggctgtga
    11341 gctgtgggcg cgggccgctg taccggcggg accggctgct tcggccggtt cggcgggttt
    11401 caccggctcg gcgggggcca ccggctcggc tgctccggtg gccggtggcg cccgcgcctg
    11461 aagaggacga agaggacgag gacgggcacg atgaggacgg gaaccatgac gttgaaactg
    11521 cgcgccgatg tgtcgacggc cgacaccgag tacggaacgg tcctgctgga cggctccacc
    11581 ggccgctact ggcagctcaa cgccaccgcg agcctggccc tgcggaccct cctggagggc
    11641 gggacggccg aggaggcggc ccggcggctg acgaccggtt acgacgtcga ggagtcacgt
    11701 gcccgcgccg acgtggaaaa gctgctggcc ggactgctcg ccgccaaggt ggcggaggcg
    11761 tgagcacggt cgtaccgcat cgcgcggacc tgcggctcac gcccggacgg cggctgctca
    11821 ccgcgctcgc ggtgtatgcc gcccgccgtc tggcacgcaa gccgccggct catatacgca
    11881 ccgtactgac ccggttgcgc aagggtgccc gccccgccac ctacgccgag gcgcagaccg
    11941 cccgcgaggc cgtactcgcg gtgagcctgc ggtgtgcggg cccggaaggc tgcctgcccc
    12001 gctcgttggc gaccgtgctg ctgtgccggg cgggcggcac ctggccggcc tgggtggtgg
    12061 gggtacgggc ccgtccgccg ttcggggcgc atgcctgggt ggaggccgag gggcggatgg
    12121 tcgacgaaga ggcgggaccg gagtacttcc gtgcgctgtt cacggtcccg gcggcaaggt
    12181 ccgcaggagc ggagcagaac ctcggaaaaa cgagcggaac ctcgaagcag cagtgagaac
    12241 aaccgacaag tagagaccgt tggcgggaag tgggggtcac aaggtgcgat gttgtacgtc
    12301 ccgtcgcacc ctggggcatg gcagcgagtc cgcccgtgat cgtccatccg cccaccccgt
    12361 ccggcgggcg ccgcgtcacc ctgcgcgggc ggttcgccgg gctggcgcac ggccggggcg
    12421 atgtcgccga gttcctccgc cgggccggtc tggcgccgga ctccgaggac atcgacctgg
    12481 acgatcccga gctgatcgag tggcgcggtg gtgacctcga cacatggccg gagccgcagg
    12541 ggtgaacccc cgtaagacga tcgccggagg cggagagtgt gtgactgaac acactgcgcc
    12601 aacgaggtta cccgggaggt aacggaacac gactggtcag gaggccgcgt ccggccgtgt
    12661 cgcgacggag gggcgcgcgg gccgcggccg tgctccgctt cgcggggtgc gttgtcagtg
    12721 gctccctcta gcatgtctcc catgtcccca actccccctg gctgtgccgg cacgtcgctt
    12781 cgcgccgccg aggcgaacga ggcgatacga cagttcgtac gcgcccgccg cgggcatgtc
    12841 tggactgcgg aggaccggtc gcggtaccgg cagttgctgg agacgtggct ggtggccgtc
    12901 cacgggtcgg tcagcggggc ctgaacggcg agcggtcacg gacaccgccg ccccgccacc
    12961 gtacggcgcc atccgcgcac gtcaccacct cctcacacca tcaacatcac tccccctcct
    13021 cgcatcatct cccatctcca cgtccccgca acgattccgc gcatcatctc cggacatcgt
    13081 ctccgcgggt catctccacg tccgatttcc gccagcactc atgcccggcc cggcgcacag
    13141 tagtaagcgc ggcccacgag aatcggaggg gaccgactgc gcctcgctcg tgcctctccg
    13201 gcttcccgcc ggcctgtccg gcccgccgcg cctcactgaa cgcgatacga aagcacccca
    13261 cctcatggct ctccatacga ccgcccccgc caccgccttc cctcccaccc ctcccagcac
    13321 tgtcaccgcc accgccaccg ccaccgccac tgccgcggga agcagcacgc tctacgcgtc
    13381 gatcggcagt ccgctcggtg aactgctcct ggtcggcgag gagagcccga ccgcgcgcgg
    13441 cggcatcgcc ctggcctcgc tctccatgtc cggccagaag ggagccgcga tcgtgcagga
    13501 cggatggcgg cgcgcccccg aggcgttcgc cgagatcgcc gagcagctag cggcgtactt
    13561 cgacggagcg cgcacccatt tcgacatcga ccgcgtcatc ggccggggca ccgcgttcca
    13621 gcagcgggtc tgggacgcgc tggagaccat tccgtacggc accaccacca gctacggcgc
    13681 gctggccgag gcagcgggca tctcccgtgc cgcggtccgc gccctgggta cggcgatcgg
    13741 tgccaatccg ctgctgatcg tacggccctg ccaccgcgtc atcggcgcga acggcaccct
    13801 gaccggctat gcgggcgggc tggagcgcaa gcggcggctg ctcgacctgg agcaggcatg
    13861 accacccgtg tcccgtcggc ggtggacagc gcggacaggc cgggccgtcc cgcctggaca
    13921 gacgatccag gtgggacgga cgagccaggt gggacggacg ggacggaccg gctgggccgg
    13981 gccgactggc cgtccctgac cgatgagctc aacgcgtacg gctgtgcgct caccccgcgc
    14041 ttgctgagta cccgggaatg ccgggagatc gcggagctgt acgacgaggc ggaacggttc
    14101 cgcgcaaccg tggacatggc ccggcaccgc ttcggttccg ggcagtaccg ttacttcaac
    14161 cgtccgtacc cggagcccgt ccagcgactg cgcgaggcgt tctatccgcg cctgctgccg
    14221 atcgcccgcg actgggcgga gaaactgcgc aggccggcgc cctggccgga cacgctggag
    14281 gagtggctgg cgatgtgcca ccgggccgac caggccaggt cgacggcgat cctgctccgc
    14341 tacgaggagg gggactggaa cgccctccac cgcgatctgt acggcgatct ggtcttcccg
    14401 ctccaggtcg tcatcgggct cgacgaaccg ggcaccgact acacgggagg cgagttcctg
    14461 ctggtggagc agcgtcctcg tgcccagtcg cgaggcaccg cgaccgttct ccggcagggg
    14521 cacgggttga tcttcaccac ccgtgaccgg ccggtccgtt ccacgcgcgg ctggtccacc
    14581 gggccggtgc ggcacggcgt cagtacggtc cgctcgggcc gccgccatgc gctgggcctg
    14641 gtcttccacg acgccgcctg acagctcccc gcccgctctc gtctccgtcc gccgcggcac
    14701 tcaccatgag aagtaacgac ttcgcaagca tcgagaaggg atgaaaccgg ccgaatgacc
    14761 gcatcgccgc agcccggagc gcacagaccg tcccggcggc ccatgccgcc cggagatctc
    14821 acactgcccg gcttcggccc cccttccgat tccaatcctt ccgattccgg cccttgcgat
    14881 tccgcattct catccgcctg cggccccgcg gcggacgcgg tgtacgatca cggctccccc
    14941 tcccgacccc gcacgagccc cgcgccgggc gcggtgcacg tccccgcctg gctgaccttg
    15001 gagcagcagc gggaactggt caccgcctgc cgggactggg cgcgcggtcc ggtgccgatc
    15061 cggcacacca aactgccgcg cggcggggtg atgtccgtac agacggtgtg catcggctgg
    15121 cactggcagc cgtacgcgta cacccgtacc gccgatgacg tcaacggcgc ccgcgtcgcc
    15181 gaattcccgg actggatggt ggagttgggg cgccgggcgc tggtcgacgc gtacggggag
    15241 gagagcgccg gtgacggcta cacaccggat acggcactga tcaacttcta tgacggggac
    15301 gggcggtcca aactcggcat gcaccaggac aaggaggagc ggtccgacgc gccggtcgtc
    15361 tcgctcagca tcggtgacac ctgcgtcttc cgcttcggga acaccgaaac ccgcacccag
    15421 ccctatacgg acatcgaact ggcctccggt gacctgttcg tcttcggtgg accgtcccgg
    15481 tgggcgtatc acggggtgcc gaagacgtac ccgggcaccg gtgaccctgc caccggcctg
    15541 gcccgcggcc gtctgaacat caccatgcgg gtgacaggtc tcccgtaacc gccggctacg
    15601 gccgcctgcc acccattcct cccgctcccc ccttccctcc cccgtgccgg cgccgggccg
    15661 aggacgcaca ctggtcccat gtgccgaagc atcaagacac tccgtccgcc cgccaccccg
    15721 gaagtgaccg acgaggacat ccgcgcagcg gcgctgcagt acgtccgtaa ggtctccggc
    15781 ttccgtgcgc cggccaccca caaccgcgag gtgttcgagc acgccgtgga cgcggtggcc
    15841 gccgcgaccc aggacctgct cgcgggcctg cgcgtccgcg gcagctcagc tcactgatcc
    15901 cggctccggc ccgggcctcg gcctcgggcc ggagctcagg ctctgcctcg gcctcgcgta
    15961 ggttgccggc gtctcacaac tccaccagcg cctgcaccgg caggtgatcg ctggggaact
    16021 gtccgtcccg cgagaaggtg ttgatgtccg cacggcggat acggacggag ggcgaggtga
    16081 ggatccagtc gatccggtcg ccgtccggta ccagcggccg gtagccgtga aatgtcgcgt
    16141 acgcggagcc acgcgcctcg gcaccgtccc aactgtccag cagcgctcca ccgtcgagca
    16201 tggcggtgta gacggggttc ttgtgcgcgg ggacgttgaa gtccccggtg acgatgcgcg
    16261 gcagcgccgg gtccagcgca cgcaggcggt cggtgatcag tgccgccgac cgttcacggg
    16321 cgtactggtg ggcatggtcg agatgggtgt tcagtacgga gaactcggcc tccgtccgca
    16381 gatcccggaa gcgcacccac gtgaccatgc ggatgacggt gttgccccag gtcgccgaac
    16441 cgatcagata cgggctgtcg gacagccaga agtggtcgta ctcgatcggc tccaatctgc
    16501 ggacgtcata gaagaccgcc acgaactcgt cgcggctgcc cccggcccgc cccgttccca
    16561 gccaggcgta gtcgtcaccg aggtcctctg ccatctcgcg gagctggccg tacagccctc
    16621 ctgcgtaccg agaaggtgcg gagcggcggc ccgcagcagt gcgcgcatga cgggccggcg
    16681 ctccggccac gaatggggcc ctacgtccga ggcgtagcgc acgttgaagc tcatgacccg
    16741 cagaccgggg cccgccgcgc gggcagggga ggaggcaggg gcggagctgg ggccggcatc
    16801 ggcgtcggcg ccggtgccgg tggtgccagg ggcgattcgc tccccgaact cgggatcgtg
    16861 accgatacca gccatgccga ctccgatcta tccgggacct ttccaggacc tttccgggac
    16921 ctttccgaca cttctcgtgg atttccccgg cgacccaccg cctttgcccc cctccccctt
    16981 cccttgctcc tgccctctcc cctgcgtcta agccgtcact cggccgccgc tggcccctgc
    17041 cagtcggcga accggatacg ggacagctcc cgtacgtccc cgatcgtccc cattcgtcct
    17101 tgcccagccg cgagagcggc cgaagcttcg tgatctcggg gtgtccgtcg accatcacct
    17161 cttccgaaac agccgcatgc accacccgtc cgaagaccac ggtggaatca ccgagcagca
    17221 atgtgctgtg cagctcgcat tcgaacgcca ccggcgacgc cgcgacccgc ggcggcttga
    17281 cgcgcaggct cggttcactg tcgataccgg cccgctcgaa ctcgttcacc ccacgcggga
    17341 agtcgacggc ggtgtcattg atctgccgga aatgccgctc gtccgccagg ttgaccacga
    17401 actgcccggt ttcctcgaca ttgcgcagcg aatccttacg gcccaccgcc gtgaactgaa
    17461 cgatgggcgg agcgacacag gaaatggtga agaacgagtg cggggcgagg ttgggcacgc
    17521 cgtcccggtc gatcgtcgaa acccaggcaa tgggccgcgg aaccaccgtc gcggtcagca
    17581 gccgatagaa atcgttacgg gaaacggcag cgggatcgaa gtcaatacgc atgaggagca
    17641 gtatcccctg cgtacggact cgtcccgccc ctggtccgga ccttgggcgc ggcgccgcgc
    17701 ctcgttcggg aaccgcttgg ccaagcatga gcctgaagca tcagcctgct accgctcctt
    17761 cctccctcac cgcccctccc cgccaccccc atccggtatc tcttggctgt tccccgctac
    17821 cgggtcatcg ctgtgtggga gggtgccagc gtctgggacc gataccggga gggccacagt
    17881 ggaccgtgag cgtgaggaac gccgaaatac ggatcgccga agtacggatc gccgaagtac
    17941 ggatccccaa catggggaac gcgagcagca cggaagcaag gcaccgcacg aggggccgta
    18001 cgcggccgac cggccgggta tgtgccctcc gctccaggga cacatcgacc gcgccacgga
    18061 attcgcgccc gaggacgagg aacggcagcg ccaggatttc ctggccggca cggtgcccgc
    18121 ggccggcgcc ggtatcgccg tacccgggga cgggccgggc ggaccactgg acgcgctgtt
    18181 catgatcttc gtccacgagg accagcgcac cttcggcccg gctccccagc tcaaggaggc
    18241 attcgccctg ctggaccggt acggggcggt gggcctccac gaccagttgt cggtgggcac
    18301 cggctgggcc gggatgggcg gcagggaccc gctggtcaag ctcaagctgc agttccacga
    18361 cgcgttgtcc gggtcgaccg gcatcgtact ggcggcccac cggaacgcca cgggtggcgg
    18421 catgtcgtcg gcggcggcat gatcgccctc acctccctgg aacggatgga ccgggcgacg
    18481 tcccgccccg gtgcgacctt cgcggacgcg ctggaggtgt ccatcctgtt ggaggtcggc
    18541 acctcgccgg tcctggagcg catgatcgac atctacgact ggcccaggcc ctgaccttgc
    18601 tcacccctgg gcagccgtcc cgaacccgcg tcagtcagtc attcggccag tcggtcagtc
    18661 ggtcagtcgg tcagttgaag gtgaccgaac gccgaggcgg cgggaggcgg tcgagtatct
    18721 cccacagcgg ccgggagccg cccgcccact ccaccagaac cgcacggtcg accaaatgga
    18781 tcccgtgccg gtgcccccac ggtatcgcct tggacgagaa gcggccgttg gtgacgacca
    18841 cggcgacatc cgcccggtgc accggcttgg ccgtcccgtt gacctgttgc agcacccccg
    18901 tccccacggc ctgcccgcgc agcccgtccc gacggtgctt gcactgcacg gcccagaccc
    18961 ggcccaacgg gtccgtggcc ttcacgtcgc acgcgtcgtc accggcgcct cccagccgct
    19021 cggcccggca cccgtcgcgg cgcatcagat cacgtacggc cgtctcgaag tcccgatggt
    19081 ccagcgcgtc cagttgagcc ggccgcaggc gcagcgcctg gcgacggacc cggtcccacg
    19141 ccgcccgctg ccgcgcacgg tggacatacc ccccggccgt cagcgcggcc agcaccccga
    19201 ccagcgccag gacccataag tgggcaagca gccactgtgt cagcagcacg cccccgacgg
    19261 cagcggcact cccgacgccc accacggtat tccgccgctg ccgcgccttg ctgcgacgcc
    19321 ggacgggcac gcggctcacc aggcacctcc ccgcacggcg ctgttcacaa cggccggccc
    19381 accctcacac atcccgaccc gcctgacccg tacgaccacg accatccgcc tcaacccccc
    19441 tcgcgttcga gcccgttggc ttcctcacac ccaccagcac agcacacgcc actgacaacg
    19501 gcccctcccg agcacccacg cctggagcgg caccagttgc cgcgggcggc ggccggccgt
    19561 accgcgtgag gaggatcaag gaagacccgt tcgccgtgtg gtgttgtggt cgtcgatggg
    19621 ccgccgcgag cgcggggcct ggattgaggg gtatgagtga tgagtgcgga tctgtacggg
    19681 attccgttgc ggacgctggc cggggagccg gtatcgctgg ccgactaccg ggggcgggcg
    19741 ctgctggtcg tcaatgtggc gtccaagtgc gggctgacgc cgcagtacgc ggggctggag
    19801 cggcttcagg agcgcttcgg ggcacggggg ctgagtgtgc tgggcttccc ctgcaaccag
    19861 ttcgcggggc aggagccggg tacggccgag gagatcagta cgttctgctc cacgacgtac
    19921 ggcgtcagct tcccgttgtt cgagaagacg gaggtcaacg ggccggaccg gcatccgttg
    19981 tacgcggagt tgacccgcgc gcaggatgcc gagggcgagt ccggggacgt tcagtggaac
    20041 ttcgagaagt tcctggtgaa cgggcagggc gaggtcgtgg ggcgtttccg gccgcgggtg
    20101 gagccggaga gcgaggaagt ggtgcgggcc gtcgaggcgg tgctgccggg ctgaagccgc
    20161 cggggtggaa cgggtgatgc ggcactcggc ggagcgtgat gtgccgggtg ccgcatcggc
    20221 tgtggggatg aggtgaggga gggaaagatg tgggcgggtg ggcgggagtc aggcgatgac
    20281 cgagttgtcg gagttggtgg tggcggcggt ccgggggatt gcggagggag gggggtcgca
    20341 caggtcgaag caatgggtca ttccggtcag ccgcagcagg cgctgggtgc tggggctcgc
    20401 ggcgacgccc gccaggcgca gccggccgtc cctggcagcg atgcgctgcc gtgcccggca
    20461 cagcagggac aggccgccgc agtcgatgaa ggtcacctcg ctcatgtcca gtacgaggtc
    20521 gggacggcgt acgttggtga gttcgtcgat gcgctgcgag agtgtcgtcg tggcgaagat
    20581 gtcgagttct ccgctcagtt ccagtacgac gccgaagctc gtcggccgta cccggctccc
    20641 gaatgtccgc ccttcgtcca gcatggcgtc cccacctccg tacccgtgcg tattcggccg
    20701 cgcgcaggcg gtggcgggat gcgagaggtg aagggcccac ccggcgcgga accgtgcggg
    20761 aacgaccacc gtcggcgccg ggcatgcgcg cagttccagc ctccggcggg gtctcggtcg
    20821 gcggctgcgt caagcatgtc tacggttgcg cccaaccgtg tctagtactt gtctacggtt
    20881 tacccgctgg ccgcgcggct cagtccgcgc gcggcgtacg tatggaggtt ggctcaattc
    20941 agtactgtcc tgggccgact gtcatggacg gccgtggctc gttatggact gcggtggctg
    21001 gtcatggtcg gccaggggcc gcgtcgtcgc ccagggcgca gaggaacgac gggcccgggc
    21061 ggggccggag cgccggtgat cacccctggt caccagggag aggagtaggg ggcagcggct
    21121 ccgcctttac cggtggccgc gccggtccgc tctcgtggct ccggaggata cgtcgccaca
    21181 ggaggtgccg ggcggtttct cacccggtcg caggaacccg tggcgggcgc ggtggcgacg
    21241 ggtgcggacg cgtgccagcg tggctggagg gtggtgatct ggttcggacc gttttcggaa
    21301 ggggggaaac gccggacgac acccgaccga ccgaccagcc gaccaccggc cgatcgcatt
    21361 gccgacagcg gccgacagag gcgaggaccc tctcatggaa agacccagag tcctgatcgt
    21421 gggcggcggc ttcgccggtg tggagtgcgc gcgggccctg gaacacggcc tgccggcccg
    21481 ggaagccgag atcacgcttg tcacgcccaa caactaccag ctctacctgc cgctgctccc
    21541 ccatgtcgcc gccggagtgc tcaccccgca gtcgatcgcc ccctcgctgc gccgcatcct
    21601 gcgccgtacc cgcatcgtgc cgggcggggt gatcggcatc gacgccgcct ccaaggtctg
    21661 tgtcgtacgg ctgatcaccg gcgagaccac cgacgtccac tacgaccgcc tggtgctgac
    21721 gcccggcagc gtcacccgtt ccttcgacat ccccgggctg agcgagcacg cacgcggcat
    21781 gaagaccctc gccgaggccg cctacgtacg tgaccacgtc atctcgcagc tcgacatcgc
    21841 cgcggcctcc tcggacgcgg cggagcgcgc cgcccggctg cagttcgtgg tcgtgggcgg
    21901 cggatacgcg ggtaccgaga ccgtcgccta cctccagcgg ctcaccaacg cggcggccaa
    21961 gcgctacccg cgcctggacc ccgcccagat caagtggcac ctgatcgaca tcgcgccgaa
    22021 gctgatgccc gaactcggcg accggctcgg cggcaaggcc atggccctgt tgcgcaggcg
    22081 cggcacggac gtctcactgg gggtgtccgt cgccgaggtc accgcggaca ccgtgcgctt
    22141 cacggacgac cggctgctgc cctgccacac cctgatctgg acggcgggtg tcgccgccag
    22201 cccgctgatc ggcacgctgg gcgccgagac cgtacg
//
