LOCUS       NZ_CP074380            21182 bp    DNA     linear   CON 27-MAY-2024
DEFINITION  Streptomyces sp. MST-110588 chromosome, complete genome.
ACCESSION   NZ_CP074380
VERSION     NZ_CP074380.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. MST-110588
  ORGANISM  Streptomyces sp. MST-110588
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7915503)
  AUTHORS   Booth,T.J., Bozhueyuek,K.A.J., Liston,J.D., Lacey,E. and
            Wilkinson,B.
  TITLE     Bifurcation drives the evolution of assembly-line biosynthesis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7915503)
  AUTHORS   Booth,T.J., Bozhueyuek,K.A.J., Liston,J.D., Lacey,E. and
            Wilkinson,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAY-2021) Molecular Microbiology, John Innes Centre,
            John Innes Centre, Norwich, Not US or Canada NR4, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 28-FEB-2016
            Assembly Method                   :: HGAP v. 3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 25.0x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022695595.1-RS_2024_05_27
            Annotation Date                   :: 05/27/2024 05:38:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,918
            CDSs (total)                      :: 6,828
            Genes (coding)                    :: 6,327
            CDSs (with protein)               :: 6,327
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 501
            CDSs (without protein)            :: 501
            Pseudo Genes (ambiguous residues) :: 0 of 501
            Pseudo Genes (frameshifted)       :: 350 of 501
            Pseudo Genes (incomplete)         :: 185 of 501
            Pseudo Genes (internal stop)      :: 14 of 501
            Pseudo Genes (multiple problems)  :: 45 of 501
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 12:56:23
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 140246
            Orig. end                         :: 161428
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP074380.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Barrie Wilkinson, John Innes
            Centre, Norwich.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..21182
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="terpene-precursor"
                     /region_number="2"
                     /rules="(PT_FPPS_like and not PT_HPP)"
                     /tool="antismash"
     cand_cluster    1..21182
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PT_FPPS_like and not PT_HPP)"
                     /kind="single"
                     /product="terpene-precursor"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..21182
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="False"
                     /core_location="[10000:11182](-)"
                     /cutoff="10000"
                     /detection_rule="(PT_FPPS_like and not PT_HPP)"
                     /neighbourhood="10000"
                     /product="terpene-precursor"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..11182)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(PT_FPPS_like and not PT_HPP)"
                     /neighbourhood="10000"
                     /product="terpene-precursor"
                     /protocluster_number="1"
     gene            329..>1018
                     /locus_tag="KGS77_RS00715"
                     /pseudo=""
     CDS             329..>1018
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF039623.3"
                     /locus_tag="KGS77_RS00715"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="DUF5995 family protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MSVARHGDLVCQGPVLGCVRDARRTLTRLRDGLGCDHKAPFPSIY
                     VHLEQSLERAAARPGAFDEPAWLAGSLNTGFVNLYLSAYDADQAGRPVPRAWRTAFRAA
                     RSADVNAGQDVLLGANAHIQRDMPYVLARLGLRRPDGSSRKPDYDRFQAVLDRAYGPAV
                     REIARHYDPVVALADERWNPIAGYTAHELFHLWREQAWVHARRLIDADGAAARARVVHA
                     IETNAAHW"
     gene            complement(1310..2194)
                     /locus_tag="KGS77_RS00720"
                     /old_locus_tag="KGS77_00715"
     CDS             complement(1310..2194)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1039: aldo/keto reductase family oxidoreductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015036271.1"
                     /locus_tag="KGS77_RS00720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_00715"
                     /product="aldo/keto reductase"
                     /protein_id="WP_242578019.1"
                     /transl_table=11
                     /translation="MQYRTIGDTTVSAIGLGAMPLSIEDRPDEERAVATLHAALDAGVT
                     LIDTADSYHWHAGESGHNELLIARALACYSGDTSDVLVATKGGRGRPGDGSWTVTGSPE
                     HLKRAAESSLKRLGVEAIGLYQLHKPDPNVPWADSVGALRELLDAGTIRAAGISNVNTG
                     QIHEARTILGDGLVSVQNQYSPAVRDSEAELRLCTELGLAFLPWSPLGGITRSSLDGPS
                     GPASAGTAFHRIAAGRGVSPQQIALAWLLARSPVMIPVPGASRPASVQNSAQAAELVLS
                     GTEVKELDGTLPN"
     misc_feature    complement(2483..2496)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 18.28"
                     /tool="antismash"
     gene            2483..3406
                     /locus_tag="KGS77_RS00725"
                     /old_locus_tag="KGS77_00720"
     CDS             2483..3406
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1014: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009999719.1"
                     /locus_tag="KGS77_RS00725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_00720"
                     /product="LysR family transcriptional regulator"
                     /protein_id="WP_242578021.1"
                     /transl_table=11
                     /translation="MIDVQRLRVLRAVAEHGSFNQAAAALLITPSAVSQHVAALERSLG
                     TQVAARSTRGVTLTRAGQIMVEAAEAVTAELAYARQQIDRLGTGRTKLTVATFTSGGRI
                     LLPGALARLAAAHPDTEIHVMEGEPEVTLPLVRQGTADLALAYHFDGPLPVRPGDRTGM
                     EWSELLDDPLYVVLPRGHRLAGHGSLDLSELAAEPWVLGCLKTEAYLRRYAELAGFAPL
                     VRGSTTDYFFACSLVAAGVGISLVPSIALTPAPADLHIVPVGPPGPARHIGVATAARSR
                     HQPQVATLIRVLREQASAIGASRPSM"
     misc_feature    3465..3478
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 19.24"
                     /tool="antismash"
     gene            3610..3810
                     /locus_tag="KGS77_RS00730"
                     /old_locus_tag="KGS77_00725"
     CDS             3610..3810
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018540183.1"
                     /locus_tag="KGS77_RS00730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_00725"
                     /product="DUF1918 domain-containing protein"
                     /protein_id="WP_242578023.1"
                     /transl_table=11
                     /translation="MHASKGDRLVVHGRVVGKQDRVVEIVEVLGPNGTPPYRVRAENGH
                     ETIMTPGPDSVVDHRKGSGPA"
     gene            complement(3922..5835)
                     /locus_tag="KGS77_RS00735"
                     /old_locus_tag="KGS77_00730"
     CDS             complement(3922..5835)
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1293: aldehyde oxidase and xanthine dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /locus_tag="KGS77_RS00735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_00730"
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="WP_347404412.1"
                     /transl_table=11
                     /translation="MTWRKVSRRPTSSTSRPSVSPAQHAHLETHAALGRLDDAGRLVVR
                     TSTQAPFLVRRILCALFGLPEDRVRVVAGRVGGGFGGKQEMLVEDVVALAVLRTGRPVK
                     LEYTRAEQFTGATTRHSCTVTVRAGARRDGTLTALRVRAVYDTGAYGNHGAAVLACGCT
                     EPLALYRCANKKVDGYAVYTHTVPAGAFRGYGRGQVGFAVECAVDELARRLAVDPVAFR
                     RRNVIRAGDPLVTGGAAPGDDVDITGFGLDECLAAIRTRTAAAAGEAAGQAGSASGEKG
                     SAPGEEDSVHGRVDPASGGVAAEPGGPDPPSVPAPPGWLTGEGCAVSMLITSPIGGHVG
                     TVEISLEPDGTYRLTAGVPEIGSGTTTTLVQLAADALATTPGRITVHQADTGSLGHDSG
                     MFGSAGTVVTARAALRAARCLAAAICDYAAELTSTAGLTGSGCRLREDSVVCGPAPSLS
                     LAYLARTAARTSGRTLKATGRCEGTARSAGFQAHWTRVAVNPATGEIRVLDSVQAIDAG
                     HVLNPAQARGQAEGAIAQALGAALWEEVALDERGEVTTTSLRHYRLAAFAEVPVGRVRF
                     VRTSDPVGPMGAKSLSEGCYLPVAPALANAVRDATGVRFTDLPLRADRVWDTLHHSRHD
                     RG"
     gene            complement(<6017..6817)
                     /locus_tag="KGS77_RS34795"
                     /pseudo=""
     CDS             complement(<6017..6817)
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF013482.4"
                     /locus_tag="KGS77_RS34795"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MRRKGRERGEGGVRFWVRVNGRRYVKPPRAGQCLRTYLREQGWYG
                     VKKGCDTGDCGACAVQVGSMVLPSCLYPAFRAHGRTVTTVEGLGSASGALHPVQRRFLD
                     VRGFQCGFCTPGLIMTTQALGDEALADLPQALKGHLCRCTGYRAVEDAACGVRPAASTA
                     AADPAGRDIVTGAARYTLDVDPPPGMLHLKLARSPHAHARITDIDLSDALRVPGVRAVL
                     THHDDPGRHFSTAQHEHPEDDPADTRILDDIVRHIGQRVAAVVAD"
     gene            complement(6789..7634)
                     /locus_tag="KGS77_RS00740"
                     /old_locus_tag="KGS77_00735"
     CDS             complement(6789..7634)
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018100026.1"
                     /locus_tag="KGS77_RS00740"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_00735"
                     /product="FAD binding domain-containing protein"
                     /protein_id="WP_242578024.1"
                     /transl_table=11
                     /translation="MDLHTVEEVSQLGQQGRWRAGDAYLAGGTYLFAEPRPRLRRLVDL
                     GHTGWQPLRRTPAGLEIAATCTVAELVREITDTPLVAQCCRAFSSSPAVWRTATVGGNL
                     CAALPIAPMISLTATLDAVCLLYVPQDGTERTMPVTRLVTGARRTALREGELLRSVTLP
                     GRSLAGRTAFRRASLYRLGASAALVTGLLDAWDGAVSLTVTASTVRPVRLCFGPRPDGI
                     EVRAALERAVAPGEWCHDVYGEAAWRRHLTFRFAEEIRCELATEAGAAGEDEAEGAGKG
                     "
     gene            7928..8524
                     /locus_tag="KGS77_RS00745"
                     /old_locus_tag="KGS77_00740"
     CDS             7928..8524
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020114281.1"
                     /locus_tag="KGS77_RS00745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_00740"
                     /product="dihydrofolate reductase family protein"
                     /protein_id="WP_242578029.1"
                     /transl_table=11
                     /translation="MRVVISEFMSLDGIVQAPGGPDEDTDGGFAHGGWSHPFFDPEVVG
                     GAFSDALTKAEALLFGRRTWQTMAGAWPERGGEPFADRMNSIQKYVVSDTLDDDKLTWN
                     NTTRIPGDDAIARVRELRRADGGDLLIMGSPTLVRALVAEDLVDELRLMIMPVLLGGGK
                     TIFPADGRLRTLELVSSVTSDTGAHVCTYRRVAER"
     gene            complement(8694..9881)
                     /locus_tag="KGS77_RS00750"
                     /old_locus_tag="KGS77_00745"
     CDS             complement(8694..9881)
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980504.1"
                     /locus_tag="KGS77_RS00750"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_00745"
                     /product="oxygenase MpaB family protein"
                     /protein_id="WP_242578033.1"
                     /transl_table=11
                     /translation="MTTETTARYTDATLDDLRTQGDPLADATVAAMFERGEVRDFNTLM
                     RFFTTAGHKLPDGLPQSAASYLETTGMPPKWVDWDAMERARLFFMDNAAHINTGLSFAA
                     MPASYAVPRVAKLLASTHSMDYPSRRMANTGQFVTYLMQTNAFEEGSKFIPAAQKVRLL
                     HAAVRHHLTTSGRWDTERHGTPLCQEDMIGGQICFSILVLDAMHRLGIHMSEEGAEAYY
                     YAWRVVGSMLGCDMEAAPDNLTEARAYADLYLVRNLGPSEDGVRLNNQLMRMYEDVVPG
                     TIFDPIVPATIRFLVGDTIADWLQIPRSHWDTAAKAVPLVLGALETIEDSSPLGEWALD
                     KAGSLLSAFELGSLTRGRVMQYAIPEELKSDYGVRSQQARNQRWVPPAPTSAGAF"
     gene            complement(10001..11182)
                     /locus_tag="KGS77_RS00755"
     CDS             complement(10001..11182)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene-precursor: PT_FPPS_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1182: Polyprenyl synthetase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980503.1"
                     /locus_tag="KGS77_RS00755"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="polyprenyl synthetase family protein"
                     /protein_id="WP_242578035.1"
                     /sec_met_domain="PT_FPPS_like (E-value: 1.1e-25, bitscore:
                     88.9, seeds: 145, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MHDTTGFKRRIDTYLRRLADEEEAELLRVSSELAPLCAQLRSSLM
                     SGKRLRAAFLYWGWRACGQPDCDGVIKAAAAMELVHAAACTHDDIIDDSRLRHGLPTAH
                     VAFAESAFAESAFTENAFAENAFTKSQVGTSTDSGIGTGGGAGTGGREMTAGDPCGLRR
                     GRSTALAMMLGDLLMGYAGQVFATCGLPGAYVARTVPLWSSLLRETVAGEFLEILRTGG
                     VVPGVAESLEVARYKTAKYTVERPLHMGATLGGASRGVLAAFTGYGLPLGEAFQLRDDL
                     LGTFGDPAETGKSNLDDLRDKKPTALLALTVSLLSPDDRRLLAKTMAGPSLDESDAALV
                     RELMEHSGARRRTEEMIAERIARARAALDSVALPADARAALGALAVSAADRSL"
     gene            complement(11175..11492)
                     /locus_tag="KGS77_RS00760"
                     /old_locus_tag="KGS77_00760"
     CDS             complement(11175..11492)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="KGS77_RS00760"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="KGS77_00760"
                     /product="hypothetical protein"
                     /protein_id="WP_242578037.1"
                     /transl_table=11
                     /translation="MPEQEERTDAAAPAAATGRSSTAGLEQAALLAVGLADLVLERLRR
                     TAERGQQVLRRSDLREVLSDGVNDLRIRGELASRRVTPSTENYLELMACRAAKRAESTH
                     A"
     misc_feature    complement(11664..11681)
                     /note="TFBS match to HipB, Bacterial antitoxin HipB,
                     confidence: weak, score: 20.94"
                     /tool="antismash"
     gene            complement(11786..13084)
                     /locus_tag="KGS77_RS00765"
                     /old_locus_tag="KGS77_00765"
     CDS             complement(11786..13084)
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /locus_tag="KGS77_RS00765"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_00765"
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="WP_242578039.1"
                     /transl_table=11
                     /translation="MDQGALITELAESRHDDAATARIVRQLAEPGAEAVPGLVAALPTV
                     PRKVMLELVKALRLIGPPAFDAVLAAIARDEEVPDRWELGQAMRAFDARCIDRYVAALA
                     HPRIDIRQEALWGLGNIGAEAAGAVPAMLPFLDDRDSRTRHRAGEAIRSIGRSAAPRLR
                     EIRREGPAHLRRQALAALALVGGEADIDAKDLRALERLVRIKITQDEPQSLPEYRWLAV
                     PAATYEGLFDVMGLHDRRPCTISMGLSAMEDDMALVQAADGKKELVYRVFVTPELDGWR
                     LIYADTALWEQHWDFHDIIERLSAVCGEAQVYFQDDHSDSMLWAVAKDGVLERGYWRYS
                     DPEWIGEPLDWEKQVKDDDDDSSLDEEGEDERDDDLDSVTNASGVINAGIAACHLSIDP
                     ESVGYDTSMSGHGWLAVTAEGVGHTPFTGVLRI"
     misc_feature    complement(13107..13119)
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 19.19"
                     /tool="antismash"
     gene            complement(13210..13677)
                     /locus_tag="KGS77_RS00770"
                     /old_locus_tag="KGS77_00770"
     CDS             complement(13210..13677)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006124791.1"
                     /locus_tag="KGS77_RS00770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_00770"
                     /product="hypothetical protein"
                     /protein_id="WP_242578040.1"
                     /transl_table=11
                     /translation="MEAVWTSVVAVGGTLLGAVVTHVFQRLASRRSEIFTRSEALRQER
                     IATYSAYAAAVEEYRRGQADRWYRKLADPDGEAFIAARDQAHHLRTAARQALYRVTLLT
                     EDRDVVLAAERAYQCTWEISTAQVQSEQDTLDASSRQAIEAFVSRAASLVR"
     gene            complement(13867..14604)
                     /locus_tag="KGS77_RS00775"
                     /old_locus_tag="KGS77_00775"
     CDS             complement(13867..14604)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="KGS77_RS00775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="KGS77_00775"
                     /product="hypothetical protein"
                     /protein_id="WP_242578042.1"
                     /transl_table=11
                     /translation="MRELDGFEDELIERFKEHPVLANIPRLPDEDFAAILLQRRFVSLA
                     FTPSYDLAIDLLRDEVGLRIARTILREEYPDSHGNTRSHREDMKEDLLQLGISRRALVE
                     TKPTEATKKAINDTFELIADAGARDADADVRLLTILRFWGEVLVSVEYGRLWERIGPLL
                     TRNGENRSRFYYPHYVHDAKAHPLATVSLLSTTHSDRLATRLSELLARQGSTACFREAE
                     ERSLQLKTRFYDQFLLAPDRVGT"
     misc_feature    complement(14119..14121)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(14625..15467)
                     /locus_tag="KGS77_RS00780"
                     /old_locus_tag="KGS77_00780"
     CDS             complement(14625..15467)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="KGS77_RS00780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="KGS77_00780"
                     /product="hypothetical protein"
                     /protein_id="WP_242578044.1"
                     /transl_table=11
                     /translation="MGSGIRYRVERIALALMATAGIVVLLADLLGWLDKLAPGGALPKI
                     TLLILSTVTLFLLLEVDRLKVLDHVNTQLSKLDIDAVARDLKQEHYGGVVQVHRHFPEE
                     KFTGLVEAAGREVTILQTWVPNLHWFSSALRKAIVDRRVQVRILLLHPASPVARLRDEA
                     LRTVRDPALDEDVKASVERCLSILESIFREVPEGDRARLQVRVYNSLPSVAVYKADEHY
                     LVSSFLHGQLAIDSTQIEIDGGETVMGRGVQKELDMLWRVGCDVDLRDWRASVNTISL"
     gene            15904..17151
                     /locus_tag="KGS77_RS00785"
                     /old_locus_tag="KGS77_00785"
     CDS             15904..17151
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1282:
                     extracellular ligand-binding receptor"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014146253.1"
                     /locus_tag="KGS77_RS00785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_00785"
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="WP_242578051.1"
                     /transl_table=11
                     /translation="MRVRRRACGEEGRAEHGTAHGADLVVGVVAARTGRLASLGDPLAF
                     AVSLVEPRLTRITNGHRTHAVRLVCRDSRSTAEGARRAVRELVADEGARIVLTLAGTQV
                     LPAAADACEEAGVPCVSSTFPWQVYYYGRGASDVAPFRWTYHFCWGLDAIAATFADLWE
                     LAGGPGRTVGCLWNDGPQGGWSRDTERGFAGTARARGHHLVDPAAYSEPAADLSGHVAA
                     FEAAGTDIVTSAATGRDLALFRATAVERGSTPRLITCSRWLAYPPSATSGDRQRPAQAN
                     VATLVYWTPSHPYRSSLDGMTAAQLAEAYEQATGRQWLQPLGPAYALFEVAAYALRTAE
                     DPTDPAAVAAAVGRTRLETVAGTLDWTAGPVPNVATVPLAGGQWQPGRRHDYELAVLTN
                     PHVDGLVADADLMPLR"
     gene            17237..18022
                     /locus_tag="KGS77_RS00790"
                     /old_locus_tag="KGS77_00790"
     CDS             17237..18022
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /locus_tag="KGS77_RS00790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_00790"
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="WP_242578053.1"
                     /transl_table=11
                     /translation="MPARPDGRDTVRVLDVRSADYRRAFELLLAGTDEKAVTHARLSAL
                     VARLPARSVFVDIGAGAGDTTAHVGRSFARTVAVEPNASLREALRRACPEAEILPRPVE
                     SVELPAGSADLVLCCHVLYYLPRATWAATVRRMLGWLRPGGEIWILLQNADNACLRMVR
                     HFTGAHFDLAPLAAELREGRDGPVAACSLERLPCAYRTARLDDAVDVAEFMLNVPSLAR
                     QDPLPALQELCDYVRRECADPAGGYRIDHSQDLLRVRRA"
     gene            complement(18323..19510)
                     /locus_tag="KGS77_RS00795"
                     /old_locus_tag="KGS77_00795"
     CDS             complement(18323..19510)
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /GO_function="GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1006: acyl-CoA dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019067356.1"
                     /locus_tag="KGS77_RS00795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KGS77_00795"
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="WP_242578054.1"
                     /sec_met_domain="Acyl-CoA_dh_N (E-value: 2.2e-12, bitscore:
                     46.2, seeds: 790, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_1 (E-value: 8.2e-06, bitscore:
                     24.7, seeds: 49, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTIDEATVPTTAPDTARSLRPVLERHAAETDAAAVFPLASVEALR
                     AGGLFGLLVPRRYGGLGGDLRDLTEVAQILAGGCLSTAMIWATHCQQTDVLVRHAGPRL
                     RDALLPRIARGELYLTSVTTGPDEVGHLLGATAPLRRQGRLLSLDREAPVVTGGEHADG
                     FLVTMRADENSTGNRVSLVYADRAQLRLEIRTGWDALGMRGTRGVGVRLRGDVPADQVV
                     GEPGGFRDVATDSMTVTGHLGWAACWLGTARSALAGLVGLPRSPRRQPGAEPRSDLAAE
                     RLARVRLDLELAGAYLHRVRDEVAALRTAGEPLGTPAVQIHLNTLKVAVSELTFSAVDR
                     MIQLTGLPTGYLKDAPVPLERHLRDLRSAALTCANDHLLTATGPLVLLDRPVQLA"
     misc_feature    complement(19801..19812)
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 18.46"
                     /tool="antismash"
ORIGIN
        1 caacggtcaa cggtcaacgg tcaacggtca acggattgat catgcaggga acgcggtgcc
       61 ccggccagcc ttgttgccac ttcggcaaca ccactcggcc tcgaacgcag cccccgccct
      121 gccgagtacg ccgcgtacac cccgtacgcc gctcgttgcc cgtacgcgaa tcgctcgttg
      181 gtcccctcga cgcgctcgac cggccgcgac gacccgacaa gcggggggca gaacggtgtt
      241 caagctcagg acagccagag tggcgggtgc gctcgcgctg tcggtggtgc tgtccatgac
      301 gggaacggtg gccgccgatg cctcccagat gtcagtggca cgccacggcg atctcgtctg
      361 tcaggggccg gtcctcggct gcgtacgcga cgcgaggcgc accctgaccc gtctccgcga
      421 cggcctcggc tgcgaccaca aagccccctt cccctccatc tacgtacatc tggagcagtc
      481 gttggaaagg gccgccgcac ggccgggcgc gttcgacgaa cccgcatggc tggccggcag
      541 cctcaacacc ggattcgtga acctgtacct gtccgcctac gacgcggacc aggccggcag
      601 acccgtgccg cgcgcctggc ggaccgcctt ccgcgccgcc cgcagcgccg acgtcaacgc
      661 cggccaggac gtgctgctcg gcgccaacgc gcacatccag cgcgacatgc cgtacgtcct
      721 ggcccggctc ggcctgcgcc gccccgacgg ctcctcacgc aagccggact acgaccgctt
      781 ccaagccgtc ctcgaccgcg cctacggacc tgccgtacga gagatcgccc gccactacga
      841 cccggtggtg gcgctggccg acgagcgctg gaacccgatc gccggctaca ccgcccacga
      901 actcttccat ctgtggcgcg agcaagcctg ggtccacgcc cgccggctca tcgacgccga
      961 cggcgccgcg gcgcgcgccc gtgtcgtcca cgcgatcgag acgaacgcgg cccactgggc
     1021 cggtctcctg gccgtcgtcc aagtccccgg ctaccgctcg atccgcgaca cgtactgccg
     1081 ggcccgtccg ccggcctcga cgccggtccc ggcgcggtcg ggtgggccgg gctcattctc
     1141 ctccgtcgca ccgggcgagg cggccccgcg gcccaggagg cacctgacga cgcgcccgtt
     1201 ccccgtatcc gtatccgtat gtcacccgag ccctgcactc gcgcgatgga ggagttgcac
     1261 ctcctcttca gccgaccggg ctccaaggcg agctgccctt ccaagggtcc tagttcggca
     1321 gcgtgccgtc cagttccttg acctccgtcc cgctcagtac gagttcggcg gcctgggccg
     1381 agttctggac ggatgccggg cggctggcac ccggcaccgg gatcatcacc ggtgaacggg
     1441 ccagcagcca ggccagggcg atctgctgcg ggctgacccc gcgtccggcc gcgatccggt
     1501 ggaaggccgt gcccgcggag gccgggcccg acgggccgtc gagagagctg cgggtgatgc
     1561 cgccgagcgg gctccagggc aggaaggcca ggcccagctc cgtgcacagc cgcagttcgg
     1621 cctcgctgtc acggacggcc ggcgagtact ggttctgtac ggagaccagc ccgtcaccga
     1681 ggatcgtccg cgcctcgtgg atctggccgg tgttcacgtt ggagatcccc gcggcgcgga
     1741 tcgtgcccgc gtcgagcagt tcgcgcagcg cgccgacgga gtcggcccag gggacgtttg
     1801 ggtccggctt gtgcagttgg tagaggccga tcgcctccac gccgagccgc ttgagcgagg
     1861 actcggcggc gcgcttgagg tgttcggggc tgccggtgac cgtccagctc ccgtcgccgg
     1921 gccggccccg gccgcccttg gtggcgacga gtacgtccga ggtgtcaccg ctgtagcagg
     1981 cgagggcacg ggcgatgaga agttcgttgt ggccgctctc gcccgcgtgc cagtggtagc
     2041 tgtcggcggt gtcgatgagc gtgacgcccg cgtcgagggc cgcgtggagg gtggcgacgg
     2101 cccgctcttc gtccgggcgg tcctcgatgg acagcggcat ggcgcccagg ccgatggcgc
     2161 tgacggtggt gtctccgatg gtgcggtact gcatgggtgg tgttctccag ctcgggcgac
     2221 gggcagggca ggtgtggcgg gggtagtggg tgggtgcagc aggtgtggtg ggggccgggt
     2281 gcggccccgg aagacccggg cggcgacggc ggccggcacc ggggtgtagc agacctgatc
     2341 gacgaccacg tcgaaggtgc gggcgcccgg cgccgcgccc cggctgccgc cgatgacgca
     2401 gatgctttgc atgcgcccac cttggcaacc taggctcatc agcagaagta ccggcttact
     2461 gcacaggcgt taaggaaaac tgttgatcga cgtccagcgg ctgcgggttc tgcgagcggt
     2521 ggccgagcac ggcagcttca accaggccgc cgcggccctg ctcatcaccc cctcggccgt
     2581 ctcccagcac gtggcggccc tggagcgcag cctcggcacc caggtcgcgg cgcgcagcac
     2641 acgaggcgtc accctcaccc gggccggtca gatcatggtc gaggcggcgg aggcggtcac
     2701 cgcggaactc gcctacgcca ggcagcagat cgaccggctc ggtacgggcc ggaccaagct
     2761 gaccgtcgcc acgttcacca gcggcggcag gatcctgctg cccggtgccc tcgcccggct
     2821 tgccgccgcg cacccggaca ccgagatcca cgtcatggag ggcgagccgg aggtcaccct
     2881 gcccctggtc cggcagggca ccgcggacct cgcgctcgcc taccacttcg acggcccgct
     2941 cccggtgcgg cccggcgacc gtacgggcat ggagtggagc gagctgctgg acgatccgct
     3001 gtacgtcgtc ctgccccggg gccaccggct ggccggccac ggctccctcg acctgtccga
     3061 actcgcggcc gagccctggg tactcggctg cctcaaaacc gaggcgtacc tgcggcgtta
     3121 cgccgaactc gccggcttcg cccccctggt acggggcagc acgaccgact acttcttcgc
     3181 ctgctcgctc gtcgccgcgg gcgtggggat ctcgctggtg ccgtccatcg cgctcacccc
     3241 ggcaccggca gatctccaca tcgttccggt cggaccgccg ggcccggccc gccacatcgg
     3301 tgtcgccacc gccgcccgca gccgccacca gcctcaggtc gccacgctga tccgggttct
     3361 ccgggagcag gcgtcggcca tcggtgcgag tcgtccatcg atgtgaggtg gccggcacta
     3421 ggtgaccagc gcgaggtgac cggcaggcat caggaacaag tcccgtcact cgccgtgacg
     3481 gtcgccgaat agccctcatt ccgtacgtgc gcggatacgc tctgtcccaa cggcgcggcg
     3541 cgatcgccca acggcgttca tcggtcaccg cgcacggact caacggactc agcagagagg
     3601 gacgcaacga tgcacgcctc aaaaggggac cgtctggtgg tgcacggacg agtcgtgggc
     3661 aaacaggatc gcgtcgtgga gatcgtcgag gtgctcggcc cgaacggcac gccgccctac
     3721 cgcgtacggg ccgagaacgg ccatgaaacc atcatgactc ccggaccgga ctccgtcgtc
     3781 gaccaccgca agggatcggg cccggcctga cggcggcacc acgccgcatc accaccacgc
     3841 caagggcccg cccacgcggg cccttgtgca ccggcggaca gaacagagga acggagcaga
     3901 ggaacggtgg gagaggccgg ttcagcctct gtcgtgccgg gagtggtgga gcgtgtccca
     3961 cacacggtcc gcccgcagcg gcaggtcggt gaaccggacg ccggtggcat cgcgtacggc
     4021 gttggccagc gccggagcga cgggcaggta gcagccctca ctgagggact tggcgcccat
     4081 cggtccgaca gggtcggacg tccgcacgaa acgcacccgg ccgacgggta cttcggcgaa
     4141 ggccgccagc cggtagtggc ggagcgaggt ggtggtgacc tcgccgcgtt cgtcgagggc
     4201 gacctcttcc cacagtgccg cgcccagggc ctgggcgatg gccccctcgg cctgtcccct
     4261 ggcctgcgcg gggttgagca cgtgaccggc atcgatggcc tgcacgctgt ccaggacgcg
     4321 gatctcgccg gtggccgggt tcacggccac ccgcgtccag tgggcctgga agccggccga
     4381 gcgtgcggtg ccctcgcagc gtccggtggc tttcagggtc cgtccggagg tacgggcggc
     4441 ggtccgcgcc aggtacgcca gcgacagcga aggcgccggt ccgcacacca ccgagtcctc
     4501 ccgcaggcgg cagccggagc ctgtgagtcc ggccgtagag gtgagttcgg cggcgtaatc
     4561 gcagatggcc gcggccaggc atcgggctgc ccggagggcg gcacgggcgg tgacgaccgt
     4621 accggcggaa ccgaacatgc cggagtcgtg gccgaggctg ccggtgtcgg cctggtggac
     4681 ggtgatgcgg ccgggtgtgg tggcgagggc gtcggcggcg agctgcacca gcgtggtcgt
     4741 cgtgccgctg ccgatctctg gcacgccggc cgtcagacgg taggtgccgt ccggttcgag
     4801 ggagatctcc accgtaccga cgtgtccgcc gatcggactg gtgatgagca tggacacggc
     4861 gcagccctcg ccggtgagcc agcccggcgg cgccggaact gacggcgggt ccggcccgcc
     4921 gggttccgca gccacgccgc ctgatgcggg atcgacccgg ccgtgcaccg aatcctcttc
     4981 gccgggagcc gagcccttct cgccggaggc cgagcctgcc tggccggctg cctcgccggc
     5041 cgctgccgcc gtacgggtac ggatcgcggc gaggcactcg tcgagcccga aaccggtgat
     5101 gtccacgtcg tcccccggcg ccgccccgcc cgtgaccagc gggtcaccgg cgcggatgac
     5161 attgcgccgg cggaaggcga ccgggtcgac cgccaggcgg cgggccagct cgtcgacggc
     5221 gcactccacg gcgaagccga cctggccgcg gccgtaaccg cgaaaggcac cggccgggac
     5281 ggtatgggtg tagacggcat aaccgtcgac cttcttgttg gcgcaccggt acagcgcgag
     5341 cggttcggtg cagccgcagg cgagcacggc cgcgccgtgg tttccgtagg ccccggtgtc
     5401 gtacacggcc cgtacgcgca gcgcggtgag ggtgccgtcg cgacgggcgc ccgcccggac
     5461 ggtgacggtg caggagtgac gggtggtggc accggtgaac tgttcggcgc gggtgtactc
     5521 cagtttcacc gggcggccgg tgcgcagtac ggccagcgcc acgacgtcct ccaccagcat
     5581 ctcctgcttg ccgccgaaac cgccgccgac ccggccggcc accacccgta cccggtcctc
     5641 cggcaggccg aagagagcgc acaggatgcg ccggacgagg aaaggggcct gggtgctggt
     5701 ccggacgacg agccggccgg cgtcgtcgag ccggccgagc gccgcgtgtg tctccagatg
     5761 ggcgtgctgg gcgggggata cggaaggtct gctcgtactg gacgtcggcc tccgcgaaac
     5821 ctttctccac gtcacccgcc tccccgtgga tctcggcggc gatgttgcgc gaggggcggg
     5881 cgatgccgga cccggggccc ttgtcgtgga cgacgggcgc acctggttcc atggcgcgtt
     5941 cggggtcgtg gacggccggc agcacttggt aggtgacgcg cagacggcga cagccttcct
     6001 cggccgcggc ctcggtgtcc gccacgaccg ccgccacccg ctgtcctatg tggcgcacga
     6061 tgtcgtccag gatccgggtg tcggccgggt cgtcctcggg gtgttcgtgc tgtgcggtgg
     6121 agaagtgccg cccggggtcg tcgtggtgcg tcagtacggc gcgtacgcct ggcacccgca
     6181 gcgcgtcgga gaggtcgatg tccgtgatgc gggcgtgggc gtgcggcgat cgtgccagct
     6241 tcaggtgcag catccccggt ggcggatcga cgtccagggt gtagcgggcc gctccggtga
     6301 cgatgtcgcg gcccgccggg tcggctgctg ccgtcgaagc ggcgggccgc acgccgcagg
     6361 cggcgtcctc gacggcccgg tagccggtac agcggcacag gtgccccttc agggcctgcg
     6421 ggaggtcggc gagcgcctcg tccccgaggg cctgggtggt catgatcagg ccgggcgtgc
     6481 agaatccgca ctggaagccc cggacgtcga ggaaccgtcg ctgcacgggg tggagggcac
     6541 cggatgcgct gcccagcccc tcgaccgtgg tcacggtccg cccgtgcgcc cggaaggcgg
     6601 ggtacagaca actgggtagg accatggaac ccacctgtac ggcgcaggca ccgcagtcac
     6661 cggtgtcgca ccccttcttc accccgtacc agccctgctc gcgcagatag gtccgcaggc
     6721 actgaccggc gcgcggcggc ttcacatacc gccgcccgtt gacccgtacc caaaacctca
     6781 cccctccctc acccctttcc cgccccttcc gcctcatcct cccctgccgc ccctgcctct
     6841 gtggccagct cacaccggat ctcctcggcg aaacggaacg tcaagtgccg ccgccacgcc
     6901 gcttccccgt acacgtcatg gcaccactcc cccggcgcca ccgcccgttc gagcgccgcc
     6961 cgtacctcga tgccgtcagg ccgcggaccg aaacacaggc gtacggggcg caccgtcgaa
     7021 gcggtcacgg tcagcgacac ggccccgtcc cacgcgtcca gcaggccggt gaccagcgcg
     7081 gccgaggcgc ccagccggta cagcgaagcc cgccggaaag ccgtgcgccc ggccagcgag
     7141 cggccgggca gggtgaccga gcgcaggagt tccccttccc gcagagccgt gcgccgcgcg
     7201 ccggtcacca gacgggtcac cggcatggtg cgctcggtac cgtcctgggg gacgtacagc
     7261 aggcatacgg cgtccaaggt ggcggtgagc gagatcatgg gggcgatcgg cagcgcggcg
     7321 cacaggttgc cgccgacggt ggccgtccgc cacaccgccg gcgacgagga gaaggcgcgg
     7381 cagcactgcg ccaccagcgg cgtgtcggtg atctcccgta cgagttcggc caccgtgcag
     7441 gtcgcggcga tctccagccc cgccggggtc cgccgcagcg gctgccaccc ggtgtggccg
     7501 aggtccacca gccggcgcag tcgggggcgt ggctcggcga agaggtacgt accacccgcc
     7561 aggtacgcgt caccggcccg ccaccggccc tgctgcccca gctggctcac ctcttccacg
     7621 gtgtgcagat ccacgcctca ccccgccagg tcccccgagc gcaactcgca actccaccga
     7681 aacagtccac cagtcaccca gccccctccc cacgcacctt cctcacccct catccacgcc
     7741 cctgcttcca cccattcgct ccaccacctc acccatacgc cgagcaccga cgtcagggcc
     7801 cttgcccggc cggagtgcga gggcaggcgc gtgcgctcgg cgacggagga ggcggttgtg
     7861 cgccgagtca aaacccggtt cgatcagggc gcggcgagct gccggccgat acagaggagt
     7921 gagacccatg cgcgtagtga tcagtgagtt catgagcctg gacgggatcg tgcaggcccc
     7981 gggcggaccc gatgaggaca ccgacggggg cttcgcccac ggtggctggt cgcacccgtt
     8041 cttcgatcct gaggtggtgg gcggcgcctt ctccgacgcg ctgaccaagg ccgaggcgct
     8101 gctgttcggg cgccgtacgt ggcagacgat ggccggggca tggccggagc gaggtggcga
     8161 gccgttcgcc gaccggatga actccattca gaagtacgtg gtgtccgata cgctcgacga
     8221 tgacaagctg acctggaaca acaccacacg tatccccggt gacgacgcca tcgcccgtgt
     8281 ccgggagctg cgccgggccg atggcggcga tctgctgatc atggggagtc ccacgctcgt
     8341 acgcgccctt gtggccgagg atctggtcga cgaactccgg ctcatgatca tgccggtgct
     8401 cctcggcggc ggcaagacga tcttcccggc cgacggcagg ctccgcacgc tggagctggt
     8461 ctcctcggtc accagtgaca cgggggcgca cgtctgtacc taccggcggg ttgccgagcg
     8521 gtagggagaa ggcacgggac ggcgtacgct cacggcgtcc cggtacgagg cggtcggccg
     8581 ggccgccggc cgagcggtgc gtcagtcgac cggtcggccg gttcgtcggt cgatcgctcc
     8641 gccggccggc cgggcccccg ttccaccgcg atcgtccagg cgcgtgccgc cggtcagaac
     8701 gcccccgccg atgtcggagc cggtggcacc caccgctggt tacgtgcttg ctgcgagcgc
     8761 acaccgtagt cggacttgag ctcctccgga atggcgtact gcatcacccg gccgcgtgtg
     8821 agcgagccga gttcgaaggc ggagagcagg ctgcccgcct tgtccagtgc ccactccccc
     8881 agcgggctgc tgtcctcgat ggtctccagc gcgccgagga cgagaggcac ggccttggcc
     8941 gccgtgtccc agtggctgcg gggaatctgc agccagtcgg cgatggtgtc gcccacgagg
     9001 aagcggatcg tggccggcac gatcgggtcg aagatcgtgc cggggaccac gtcctcgtac
     9061 atccgcatca gttggttgtt cagccgtacg ccgtcctcgg acgggcccag gttccgtacg
     9121 aggtagagat cggcgtacgc gcgggcctcg gtgaggttgt ccggcgccgc ctccatgtcg
     9181 cagccgagca tggaaccgac gacccgccag gcgtagtagt acgcctccgc gccctcctcg
     9241 ctcatatgga taccgaggcg gtgcatcgcg tccagtacga ggatcgagaa gcagatctgc
     9301 ccgccgatca tgtcttcctg gcacagcggc gtgccgtggc gctccgtgtc ccagcgccct
     9361 gatgtggtca ggtggtggcg caccgcggcg tgcagcaggc ggaccttctg ggcagccggg
     9421 atgaacttgc tgccctcctc gaaggcattg gtctgcatca ggtacgtgac gaactgcccc
     9481 gtgttcgcca tccggcgtga ggggtagtcc atggagtgcg tcgaggccag cagtttcgcc
     9541 acgcgcggta cggcataact ggccggcatc gccgcgaagg acaggcccgt gttgatgtgg
     9601 gcggcgttgt ccatgaagaa cagccgtgcg cgctccatgg cgtcccagtc cacccacttc
     9661 gggggcattc cggtcgtttc caggtaggac gccgccgact gcggcaggcc atcgggcagt
     9721 ttgtgtcccg cggtggtgaa gaaacgcatg agggtgttga agtcccgtac ctctccgcgt
     9781 tcgaacatgg cggccacggt ggcgtcggcg agcgggtcac cctgggtgcg cagatcgtcc
     9841 agtgtcgcgt cggtgtaccg ggcggtggtc tcagtggtca cgggttcctc gtctctctca
     9901 cagggactgg tcggttgccg aacacgcagg gactgatcgg ttgcgcgcac tctcagagac
     9961 cggtcggcca tgaactcacc aggagcggtc ggtcatgcat tcacagggat cggtcggctg
    10021 ctgagaccgc cagtgcaccc agcgccgcac gcgcgtccgc cggcagtgcc acggagtcca
    10081 gcgccgcgcg ggcccgcgcg atccgctccg cgatcatctc ctcggtacgc cggcgggcgc
    10141 cgctgtgctc catcagctcc cggacgagtg ccgcgtcgga ctcgtccagg ctcgggccgg
    10201 ccatggtctt ggccagcagc cgccggtcgt ccggggagag gagcgatacg gtcagcgcga
    10261 gcagtgcggt gggcttcttg tcccgcagat cgtccaggtt cgacttcccc gtctcggccg
    10321 ggtcgccgaa ggtgcccagc aggtcgtcgc ggagctggaa tgcctcaccc agcggcagcc
    10381 cgtacccggt gaaggcggcg agaacacccc gggaggcacc gccgagggtg gcacccatgt
    10441 gcagcggtcg ctccacggtg tacttggccg tcttgtaccg ggccacctcc agggactcgg
    10501 cgactccggg tacgacaccc ccggtgcgca ggatctccag gaactccccc gccacggtct
    10561 cgcgcaggag cgatgaccac aggggcaccg tccgtgccac atacgcgccg ggcaggccgc
    10621 aggtggcgaa gacctggccg gcgtagccca tcagcaggtc gcccagcatc atggccagcg
    10681 cggtggagcg gccccggcgg agtccgcagg ggtccccggc cgtcatctcc cggcctccgg
    10741 tcccggcacc gcctccggta ccgataccgc tgtcagtact ggtgccgact tggctcttgg
    10801 tgaaggcgtt ctcggcgaag gcgttctcgg tgaacgcgct ctcggcgaag gcgctctcgg
    10861 cgaaggcgac atgggccgtc ggcaggccgt gccgcagccg gctgtcgtcg atgatgtcgt
    10921 cgtgggtgca cgcggcggca tgtaccagtt ccatggccgc cgccgccttg atcacaccgt
    10981 cgcagtccgg ctggccgcag gcgcgccagc cccagtacag gaaggcggcg cgcaaccgct
    11041 tcccgctcat gagcgagctg cgcaactgcg cacacaaagg ggcgagttcg ctggagacgc
    11101 gcagcaactc ggcttcttcc tcgtcggcca gccgacgcag gtacgtatcg atccgccgct
    11161 tgaaccccgt ggtgtcatgc atgggtcgac tcggccctct tcgccgcccg gcaggccatc
    11221 agctcaaggt agttctcggt gctcggggtg acgcgccggg aggcgagctc gccgcggatc
    11281 cgcaggtcgt tcaccccgtc gctgagaacc tcccgcagat cggaccggcg cagcacctgc
    11341 tgaccccgct ccgcggtccg ccgcagccgt tcgagcacga gatcggcgag acccacggcg
    11401 agcagggccg cttgctcaag tccggccgtg gagctccggc cggttgctgc ggcaggtgcg
    11461 gcggcgtcgg tgcgttcttc ctgttcgggc atgtccgtac tcccgtactc ccgtgccgat
    11521 gcgatcgtct gatcgtccag gaacaaccag tgaaccgatc gacgcgggcg ggtgccgctg
    11581 ttccgccact cgatcccccg cgagtggtgc gtatttcata cgaaggagtg acgatcaccc
    11641 catcccgcgg acaccgtctc ccctatccgc tatagcagtt atgccggtga ctcctgcgag
    11701 ccggtcatcc ggtcaccaac tcctgtccga ctcctgctcg caaagagcct tccgccccgg
    11761 ggcccggccg ctgccctccc cgacctcata tgcggagcac tccggtgaac ggggtgtgac
    11821 cgacgccctc ggcggtgacg gccagccagc cgtggccgct catggacgtg tcgtagccga
    11881 cactctcggg gtcgatcgag aggtgacacg cggcgatgcc cgcattgatc acccctgagg
    11941 cattggtgac ggagtccagg tcgtcgtccc tctcgtcttc gccctcttca tcaaggctgg
    12001 aatcgtcgtc atcgtccttg acctgctttt cccagtccaa aggttcgccg atccactccg
    12061 gatcgctgta gcgccaataa ccgcgctcca gaacaccgtc cttggccacg gcccagagca
    12121 tcgagtcgct gtgatcgtcc tggaagtaga cctgcgcctc gccgcacacc gcgctgagcc
    12181 gctcgatgat gtcgtggaag tcccagtgct gttcccacag ggccgtatcg gcgtagatca
    12241 gccgccagcc gtcgagttcc ggcgtcacga agacccggta gaccagctct ttctttccgt
    12301 cggcggcctg gacgagggcc atgtcgtcct ccatggccga caggcccatg gatatggtgc
    12361 aggggcggcg gtcgtgcaga cccatgacat cgaacagccc ctcatacgtg gccgcgggga
    12421 cggccagcca tcggtactcg ggaaggctct gcggctcgtc ctgggtgatt ttgatgcgta
    12481 cgagccgttc gagagcccgc aggtccttgg cgtcgatgtc cgcctcgcca ccgaccaggg
    12541 cgagcgcggc cagcgcctga cggcgcaggt gggcggggcc ttcgcggcgg atctcccgca
    12601 ggcggggagc ggcactgcga ccgatggacc ggatggcttc accggctctg tgacgggtgc
    12661 gggagtcccg gtcgtcgagg aacggcagca tggcgggcac cgccccggca gcctccgcac
    12721 cgatgttgcc caggccccac agtgcttcct gccggatgtc gatccgcgga tgtgccagcg
    12781 cggcgacata ccggtcgatg cagcgcgcgt cgaaggcacg catggcctgc cccagctccc
    12841 accggtcagg cacctcctca tcgcgggcga tcgccgccag cacggcgtcg aacgccggcg
    12901 gcccgatcag ccgcagcgcc ttcaccagtt cgagcatcac cttccgcggc accgtgggca
    12961 gcgcggcgac gagcccgggc accgcctcgg cgcccggctc ggccagttga cgtacgatcc
    13021 tcgccgtcgc cgcatcgtcg tgacgtgact cggcaagctc ggttatgagt gctccctgat
    13081 ccatggctgg acagtaaccg aacccggtga cagcgccatc gcccccggtc gacgtgcagg
    13141 cctcgccccg tcctcggtgt cacaccaggg cgccggggcc cgcccgacga gaacgtcgtt
    13201 cgcggcatct caacggacca gggaagcggc acgggacacg aacgcctcta tggcctgcct
    13261 ggaactggcg tccagtgtgt cctgttccga ctgaacctgt gcggtcgata tttcccatgt
    13321 gcattggtaa gcccgctcgg ccgcgaggac cacgtcccgg tcctcggtga gcaaggtgac
    13381 ccggtagagc gcctgccgtg ccgcggtacg gaggtggtgg gcctgatcgc gcgcggcgat
    13441 gaacgcctcc ccgtccgggt ccgccagttt gcggtaccag cggtccgcct ggcctcggcg
    13501 gtactcctcc accgccgcgg cgtacgcgct gtatgtggcg atgcgttcct gccgtagcgc
    13561 ctcggaccgg gtgaatatct cgctgcgccg ggacgccagg cgctggaaga catgcgtgac
    13621 cacggctccc aggagggtgc cgcccaccgc taccacactc gtccagacag cttccatggc
    13681 gccagccgat cacacgaaga ggcggtgcgc cgccgcatcc ggaccgcgag tggcgcggat
    13741 caccggcggc cggccccgtc gcgtacacgc tccatacgat cgacgtactt gccccggtcc
    13801 cgacccggcc acccgccctc gttacttact ccgggcctcc gctgctcgcc gccgcccgta
    13861 ctcgcgtcac gtaccgacac ggtccggtgc cagcaggaac tggtcgtaga agcgtgtctt
    13921 gagttgcagg gaacgctcct cggcctccct gaagcaggcg gtcgatccct ggcgggcgag
    13981 gagttcactc aaccgcgtgg ccagacggtc ggagtgggtg gtggacagca gggagacggt
    14041 ggcgagcggg tgtgctttgg cgtcgtggac gtagtgcggg tagtagaagc gcgagcggtt
    14101 ctcgccgttg cgcgtcagta agggccctat ccgctcccac agccgcccgt actcgacgga
    14161 gaccaggacc tcgccccaga agcgcaggat cgtcagcagc cgtacgtccg cgtcggcgtc
    14221 gcgggcgccc gcgtcggcga tcagttcgaa ggtgtcgttg atggccttct tggtggcctc
    14281 cgtcggcttc gtttcgacca gggccctcct ggagatgccg agctgtagca ggtcctcctt
    14341 catgtcctcg cggtgcgagc gggtgttccc gtggctgtcc gggtactcct cgcgcaggat
    14401 cgtccgtgcg atccgcaggc ccacctcgtc acgcagcaga tcgatcgcca ggtcgtagga
    14461 cggggtgaac gcgagcgaga cgaagcggcg ctgaagcagg atcgcggcga agtcctcgtc
    14521 cgggagccgg gggatgttcg ccagtaccgg gtgttccttg aaccgctcga tgagttcgtc
    14581 ctcaaaaccg tccagctctc gcatgtccgt atcctctccc ccgttcacag actgatggtg
    14641 ttgacggatg cacgccagtc acgaaggtcc acgtcgcagc cgaccctcca cagcatgtcc
    14701 agttccttct ggactcccct gcccatgacg gtctcgccgc cgtcgatctc tatctgggtg
    14761 gagtcgatgg cgagctggcc gtgcagaaac gagctgacca ggtagtgctc gtcggccttg
    14821 tagacggcga ccgacggcag ggagttgtag acccgcacct ggagccgtgc ccggtccccc
    14881 tccggtacct cccggaatat ggattccagg atggacagac agcgttcgac gctcgctttc
    14941 acgtcttcgt cgagggcggg atcccgtacg gtacgcagcg cctcgtcacg caaccgcgcc
    15001 acgggcgagg ccgggtgcag caacaggatg cgtacctgga cgcgccggtc gacgatggct
    15061 ttcctcagcg cgctgctgaa ccagtggagg ttgggcaccc aggtctgcag gatggtgacc
    15121 tcgcggccgg ctgcctccac caacccggtg aacttctcct cggggaagtg ccggtgcacc
    15181 tgcaccacgc cgccgtagtg ctcctgcttg agatcgcgcg ccacggcatc gatgtcgagc
    15241 ttggagagct gtgtgttgac gtggtcgagc actttcaggc gatcgacctc cagcaggagg
    15301 aacagggtca ccgtactgag gatgagcaac gttatcttcg gcagcgcgcc gccgggtgcc
    15361 agcttgtcga gccacccgag gaggtcggcg agcagcacca cgatgcccgc cgtggccatc
    15421 agggccagcg cgatccgttc gacccggtag cgaatgccgc ttcccatccc ccgctccagc
    15481 ctctgattgg ccgtcaccag cctcctggcc gaccgtagcc cccagccccc ggttggctgt
    15541 catcggcgac gaccgtacac cgcgacactg acaacaagag gcgttttgcg gcaaagcgac
    15601 aacactgacg cgagaacgcc gccggctccc cgcgccgccg gctccccgcg ccgccgggcg
    15661 ggcgacgcac agcggacagg acgatgcggc cgggctctca gctcatgtgc ttcatcccgt
    15721 ctttccttcg cctcggttcg cttccttacg caccccttcg cttcctgtcg tttcctgctc
    15781 gttctcgtac gtccgccgga agggtgaccg cgcgtacccg taccgtccgc cggccggtta
    15841 tccccaagcg cgtgcgcgtg agtgtgcacg tcacagcagg gagagcggac gtggaggcgg
    15901 acgatgcggg tacgaaggcg ggcgtgcggg gaggaaggcc gggcggagca cgggacggca
    15961 cacggggcgg acctggtcgt gggggtcgtg gccgcacgga ccggacggct ggcgagcctg
    16021 ggagacccgc tggcgttcgc cgtgtccctc gtggagccac ggctcacccg gatcaccaac
    16081 ggccaccgca cccatgccgt acgcctggtg tgccgggaca gccggtccac cgcggaaggg
    16141 gcgcggcggg cggtacgcga actcgtcgcg gacgagggcg cgcggatcgt cctgacactg
    16201 gccggtaccc aggtgctgcc ggccgccgcc gacgcctgcg aggaagcggg tgtgccgtgc
    16261 gtgtcgagca ccttcccgtg gcaggtctac tactacgggc gcggggcgag cgatgtcgcg
    16321 ccgttccgct ggacgtatca cttctgctgg gggctggacg cgatcgccgc gacgttcgcg
    16381 gacctgtggg agctggcggg cggtccggga cgtacggtgg ggtgcctgtg gaacgacggt
    16441 ccgcagggcg gctggtcacg tgacacggag cgggggttcg cgggtacggc gcgggcgcgc
    16501 ggccatcacc tggtcgaccc ggccgcgtac agcgagcccg ctgccgacct gtccggccac
    16561 gtcgcggcgt tcgaagcggc gggcacggac atcgtcacga gcgcggccac cgggcgcgac
    16621 ctggcgctct ttcgcgccac ggcagtcgag cgcggcagca caccccggct gatcacctgc
    16681 tcacgctggc tggcctaccc tccctcggcc accagcggtg accggcagcg gcccgcgcag
    16741 gcgaacgtgg ccaccctggt ctactggacg ccctcccacc cgtaccgctc ctctttggac
    16801 ggcatgaccg ccgcccagct cgccgaggcg tacgaacagg ccacgggccg gcagtggctc
    16861 cagcccctcg ggccggcgta cgcgctgttc gaggtcgccg cgtacgccct gcggaccgcc
    16921 gaggacccca ccgacccggc cgcggtggcc gcggcggtcg gccgtacccg tctggagacc
    16981 gtcgcgggaa cgctggactg gacggcgggg ccggtgccga acgtcgcgac cgtgccgctg
    17041 gccggcgggc agtggcagcc gggccgccgc cacgactacg agctggcggt cctgaccaac
    17101 ccgcacgtcg acggcctggt cgccgacgcc gacctgatgc cgctgcggta gcgcggtgag
    17161 agcaggccga gcgcgccggg acggggaagt atgcgaagca ggcacatcag cggggctcgt
    17221 ccatggggag gttgacatgc ccgcacggcc cgatggccgc gacacggtac gcgtcctgga
    17281 cgtgaggagt gcggactacc ggagggcgtt cgaactactg ctggccggta cggacgagaa
    17341 ggccgtgacc cacgcccggc tgagcgcgct cgtggcgcgg ctgcccgccc gttcggtgtt
    17401 cgtggacatc ggcgccggtg ccggtgacac caccgcacac gtcggccggt ccttcgcccg
    17461 gaccgtcgcg gtggagccca acgcctccct gcgcgaggca ttgcgccggg cctgtccgga
    17521 ggccgagatc ctgccccggc ccgtggagtc ggtcgagctg ccggccgggt cggcggatct
    17581 ggtgctgtgc tgccatgtgc tctattacct gcctcgcgcg acctgggcgg ccaccgtacg
    17641 ccggatgctg ggctggctgc ggccgggcgg ggagatctgg atcctgctgc agaacgcgga
    17701 caacgcgtgt ctgcgcatgg tccggcactt caccggtgcc cacttcgacc tggcgcccct
    17761 ggccgccgaa ctgcgcgagg gccgtgacgg tcccgtcgcc gcctgctccc tggagaggct
    17821 gccctgcgcc taccgcacgg cccggctcga cgacgccgtc gacgtcgccg agttcatgct
    17881 caatgtgccc tcactggccc ggcaggaccc gctgcccgcc ctgcaagagc tgtgcgatta
    17941 cgtacggcgt gaatgcgccg acccggccgg gggctaccgt atcgaccact cgcaggatct
    18001 gctgcgcgta cgccgtgcat gagtgcctgc gccgggcccg gtcggatcgg accgtgcggc
    18061 ctggtcaggc ggtgtccatc ggtcagggag tcgtcggtca tcgggaaact ccgtccaaga
    18121 ggggcggcgg tggacggcgg agagccgggc aggcggacgg acgcggcgtc ggccgcccgg
    18181 ctgctggggt ccagtggcgc caggagcgtc cagattccgg ccgggtcggt gaagttggtg
    18241 atgaagttga tgatgacgtt ggtggcggtc aacaggaggg gacgggccga aggtaacgga
    18301 acgctgtgga accgtggcgg cgtcaggcca gttggaccgg gcggtcgagc aggacgaggg
    18361 ggcccgtcgc ggtcagcagg tggtcgttgg cgcaggtcag cgcggccgag cgcaggtcac
    18421 gcagatggcg ttccagggga accggtgcgt ccttgaggta gccggtgggc agtccagtga
    18481 gctggatcat gcggtccact gcgctgaatg tcagctcgga taccgccact ttgagggtgt
    18541 tgaggtggat ttgcacggcc ggggtgccca gcggctcccc ggccgtacgc agtgccgcga
    18601 cctcgtcccg tacccggtgc agataggcgc cggccagctc caggtcgagg cggacccggg
    18661 cgagccgttc cgccgccagg tccgaacggg gctccgcgcc gggctgccgc cgcggagagc
    18721 gtggcaggcc gaccagtccg gccagggcgg agcgggccgt gcccagccag cacgcggccc
    18781 atcccaggtg cccggtcacg gtcatgctgt cggtcgccac gtcacggaat ccgccgggct
    18841 cgccgacgac ttggtccgcc ggtacgtccc cgcgcagccg cacgcccaca ccgcgggtgc
    18901 cgcgcatgcc cagcgcgtcc cacccggtgc ggatctccag ccgtagctgt gcccggtcgg
    18961 cgtagaccag gctgacccgg ttgccggtgc tgttctcgtc ggcgcgcatg gtgaccagga
    19021 agccgtcggc gtgttcgccg ccggtgacca cgggcgcctc gcggtccagg gacagcaggc
    19081 ggccctgtct gcgcagtggc gccgtcgcgc cgagcaggtg tccgacttcg tccggtccgg
    19141 tggtcaccga ggtgagatac agctccccgc gcgcgatgcg cggcagcagg gcgtcccgca
    19201 gccgcgggcc tgcgtgccgg acgagcacgt cggtctgctg gcagtgggtg gcccagatca
    19261 tcgccgtgga caggcagcct cccgcgagga tctgcgccac ctcggtcagg tcccgcaggt
    19321 caccgcccag tcccccgtac cgccgtggca ccagcaggcc gaacagcccg ccggcccgca
    19381 gcgcttcgac cgaggccagc gggaagaccg ccgcggcgtc cgtttcggcc gcgtgccgtt
    19441 cgagcaccgg tcgcagggag cgagcggtgt ccggcgcggt cgtcggcacc gtcgcctcgt
    19501 caatggtcat ttcttgctcc aacggggatc gcggatcggg gtacggaccc gcgtacggaa
    19561 tcgggtacgg gctcagggtc ggacacgggg catgggatgg gcgggcgcga cacagttcca
    19621 ggcgggtggc taagacggcc cggcggtgac gcagcgggtt ctcgcgtccc cggaagtccg
    19681 cgaggagccc cgcctcgcgc ccggtccggg ccggtcggcg ccacgcagtc cgcagtacgg
    19741 tgccggggtc cgcgtccgcg tccgcgggca tgccgcacgg ggaggatcga cgtgctcgtc
    19801 gcgttcggtt cccggggaca ggtgaacagg tccgcgctcc ggcgcgggcg gcagcggctc
    19861 gcggacggcc aggcccagca agagccggag agccgcacag gcggactggc ggacaggcgg
    19921 acaggcggac agcacagcgg gtatcacctg aactggtggc gacgccggtc cagatccgtc
    19981 ggtccggcct tacggacctc ccgggattcg cgttcggaca gggcagagga catgggccgc
    20041 tgactcctcg ccgcggtacg gaacgacgtc cgcagagcga gaacggggcc cctcaccttt
    20101 cccggcgtac tcgccgatgg ggtggcagga cgcggtggtg cactgcccag atgtcccaca
    20161 gtgccccatt accggaggtc gccgccaggg ggaatgcggg ggcgcgagaa gcggtacggg
    20221 ttccacggga acggccgtgt gccataccgc gtgccccttc cggtcaccgc gcgcgggcgt
    20281 gccccgacgg ggcggcgcgg gacggccgac gtacagattt cactcttgcg aagcgttgcc
    20341 tgggcgcgtc ttcgcacgac cggacggccc ggtgatcaca caccgacttc acacaccggc
    20401 ttcacacacc cgcttcgaga acccggcggg ccggcgaggc gggtggcgtc tacagccgat
    20461 gggcagtcat cgccgcccgc cctatttatc atcgtaagcc accaaataga cgccctctgt
    20521 taccttgctc tcacggatgc cacccaccgc gcgcggaccc cggcgcggcg gcgggcgctg
    20581 aattcggccg gtcgaccgaa cgacctccga caaatccagg taacgatcga acaggaatgg
    20641 acggaccatg tccggcgaga cgatcagcga tctgtacgag gtccgtgacg agcgcttccg
    20701 taccggcggc tgcgccagtg gtgacgtacg gctggagcgg ctgtactccg actgccgctg
    20761 ggccgagggg ccgctctacc tgcccgcctg gcggcagctc atctggagcg acatccccaa
    20821 cgaccgtctg ctgcgctggg acgaggcgag cggcacggtc ggcgtcttcc gctcccccgc
    20881 cgggtacgcc aacggcaaca cgctcgaccg cgagggccgg ctgatcacct gtgagcaggg
    20941 caatcgccgg gtcacccgca ccgaacacga cggccgcgtc accgtcctcg ccgaccgctt
    21001 cgagggcaag cggctcaaca gtcccaacga cgcggtcgta cgctccgacg gctccatctg
    21061 gttcaccgac ccggacttcg ggatcatcag cgactacgag gggcaccgcg cccggagcga
    21121 gaccggcagc cgcgacctgt atcggatcga cccggcggca ggccgggtcc accgggtcgc
    21181 gg
//
