LOCUS       NZ_JAKRKC020000001     10873 bp    DNA     linear   CON 09-DEC-2024
DEFINITION  Actinomadura luzonensis strain ATCC 31491 Contig00003, whole genome
            shotgun sequence.
ACCESSION   NZ_JAKRKC020000001
VERSION     NZ_JAKRKC020000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Actinomadura luzonensis
  ORGANISM  Actinomadura luzonensis
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Streptosporangiales; Thermomonosporaceae; Actinomadura.
REFERENCE   1  (bases 1 to 6358096)
  AUTHORS   Shi,X. and Du,Y.
  TITLE     Genome draft of Actinomadura sp. ATCC 31491
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6358096)
  AUTHORS   Shi,X. and Du,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-APR-2022) School of Medicine, Zhejiang University, 866
            Yuhangtang Road, Hangzhou, Zhejiang 310058, China
REFERENCE   3  (bases 1 to 6358096)
  AUTHORS   Shi,X. and Du,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-FEB-2022) School of Medicine, Zhejiang University, 866
            Yuhangtang Road, Hangzhou, Zhejiang 310058, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. v1.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 89.6x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022664455.2-RS_2024_12_09
            Annotation Date                   :: 12/09/2024 18:46:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 10,232
            CDSs (total)                      :: 10,155
            Genes (coding)                    :: 10,004
            CDSs (with protein)               :: 10,004
            Genes (RNA)                       :: 77
            rRNAs                             :: 5, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 36 of 151
            Pseudo Genes (incomplete)         :: 126 of 151
            Pseudo Genes (internal stop)      :: 3 of 151
            Pseudo Genes (multiple problems)  :: 14 of 151
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 16:31:17
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1064191
            Orig. end                         :: 1075064
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAKRKC020000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10873
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="2"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10873
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10873
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5873](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5873)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            286..804
                     /locus_tag="MF672_RS04970"
                     /old_locus_tag="MF672_004970"
     CDS             286..804
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="MF672_RS04970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="MF672_004970"
                     /product="hypothetical protein"
                     /protein_id="WP_242376477.1"
                     /transl_table=11
                     /translation="MRAVLALLALLAPLAAAACGISPTDVQDRGNAPTIRIPPPSKTIY
                     LIRDGKLALEPADVGDDTVESLLGALFDASSQPLGDRETALRGFTYLRTKDSLNPVQRD
                     EMQLPRTLTLTVYIRGEGTLSDLAKAQIVCTAQQDAAYEQVKIVRDNENRPPKDEGRYT
                     CGDLRGATS"
     gene            complement(794..2113)
                     /locus_tag="MF672_RS04975"
                     /old_locus_tag="MF672_004975"
     CDS             complement(794..2113)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020545136.1"
                     /locus_tag="MF672_RS04975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MF672_004975"
                     /product="PhoH family protein"
                     /protein_id="WP_242376514.1"
                     /transl_table=11
                     /translation="MSSSNPTTQPAKRTYVLDTSVLLADPAALTRFAEHDVVLPIVVIT
                     ELEGKRHHPELGYFARQALRYLDDLRIKHGRLDEPMPTPTGDGTLRVELNHSDPSILPV
                     GFRLGDNDTRILTVARHLAAEGCDVVLVSKDLPMRVKAASIGLTAEEYRAELVHESGWT
                     GMAEIQVTAEDVETLFEHGTADIEEARELPTHTGLRLLSARGSALGRVLPDKSVRLVRG
                     DREVFGLHGRSAEQRIALDLLMDPEIGIISLGGRAGTGKSALALCAGLEAVLERRQHRK
                     VVVFRPLYAVGGQELGYLPGTEGEKMGPWAQAVYDTLGAVTTPEVIEEVLDRGMLEVLP
                     LTHIRGRSLHDAFVIVDEAQSLERGVLLTVLSRIGANSRVVLTHDIAQRDNLRVGRHDG
                     VVAVVEKLKGHPLFAHVTLTRSERSPIAALVTEMLEDITM"
     gene            complement(2327..3088)
                     /locus_tag="MF672_RS04980"
                     /old_locus_tag="MF672_004980"
     CDS             complement(2327..3088)
                     /GO_function="GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020545137.1"
                     /locus_tag="MF672_RS04980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MF672_004980"
                     /product="isoprenyl transferase"
                     /protein_id="WP_242376476.1"
                     /transl_table=11
                     /translation="MGLRDLVYRLYERRLEAKLSRDSIPRHVGVIIDGNRRWARAMGMS
                     DVSTGHRKGADKISELLGWCRETGVEVVTVWMLSNDNLNRPREELEPLLRIIEDVTREL
                     VDEGWRIIPVGALDLLPARTANVLKNAKEASSHRPGLIVNVAVGYGGRREIADAVRSLL
                     QEQASKGASIEDLVEVLDVEHIAEHLYTRGQPDPDLLIRTSGEQRLSGFMLWQSAHSEF
                     YFHEAYWPDFRRVDFLRALRDYAARHRRYGS"
     misc_feature    complement(3095..3110)
                     /note="TFBS match to CsoR, Copper-responsive repressor,
                     confidence: weak, score: 22.0"
                     /tool="antismash"
     gene            complement(3140..3673)
                     /locus_tag="MF672_RS04985"
                     /old_locus_tag="MF672_004985"
     CDS             complement(3140..3673)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020545138.1"
                     /locus_tag="MF672_RS04985"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MF672_004985"
                     /product="hypothetical protein"
                     /protein_id="WP_242376475.1"
                     /transl_table=11
                     /translation="MGGLRCLDGSYLSVTPVACRDSVGAPYEVTLELHRDGDPYGSVGE
                     RCGWLLSRLARGVDEAREGAAPSWADPDDRFPGDEPDGELFSFRYRSRSGPAGGELSCR
                     LRTIPLWEGGRWRVTRRAYVEAWGATGVGLRAVLTSAELSSFVRGLVREAEGCLEGGDL
                     ARNAIEGAGTGETR"
     gene            complement(3702..4577)
                     /locus_tag="MF672_RS04990"
                     /old_locus_tag="MF672_004990"
     CDS             complement(3702..4577)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020545139.1"
                     /locus_tag="MF672_RS04990"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MF672_004990"
                     /product="hypothetical protein"
                     /protein_id="WP_242376474.1"
                     /transl_table=11
                     /translation="MRIVNMGEWPLDEHVHEPGGEPLWNESYYFDFVAGDGELAGYVRL
                     GLYPNWKRAWYWACLVTPGGLAASVIDHDAPLPAGGDPALTVRGAGYEASQQAGEPFGA
                     ARVRLVSDALSLDLEWRTAGGVYGYSLTPRYEIPCEVTGTIDGRPFRGHGERDHSWGVR
                     DWWSISWMWSSARLSDGTFLHGMRANLGLDLPWPAFTVPPGGELAHAGGFAAATTFDGD
                     RPAETVLRFPGLAVTARPVAFAPVTLVSPENAVAAFPRALCRFEAEDGRSGHGWTEWHQ
                     PPGWRSHAWV"
     gene            complement(4597..5004)
                     /locus_tag="MF672_RS04995"
                     /old_locus_tag="MF672_004995"
     CDS             complement(4597..5004)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="MF672_RS04995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="MF672_004995"
                     /product="hypothetical protein"
                     /protein_id="WP_242376473.1"
                     /transl_table=11
                     /translation="MSPPEEERPEEERPEEERLAEAQARLAEAQARVVRALVAAGEVPE
                     GFDQARMRAQAASLVAKRRAIVARIRPDTAVAAGPKLAAEFAAYARARTEPPPGYRADA
                     DDFAAWLRERGLMAAEDRPRRRSRWWSRLVP"
     gene            complement(5001..5873)
                     /locus_tag="MF672_RS05000"
                     /old_locus_tag="MF672_005000"
     CDS             complement(5001..5873)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020545141.1"
                     /locus_tag="MF672_RS05000"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MF672_005000"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_242376472.1"
                     /sec_met_domain="DUF692 (E-value: 1.2e-82, bitscore: 275.5,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRGDRSGGMTGERDDGNGRTELGVGIGWRSELDLTVERLAGVDFV
                     EAIAENLRPGDLPESLRLLRAKGVPVIPHGVSLGIGGAEPPSPGRLAHLAACAEALDAP
                     LVSEHLAFVRGGGLEAGHLLPIPRTRESLRVVTENVRRAQDALPVPLALENVAAIFGWP
                     DDELTEGRFLAELVERTGVRLLVDVANLYTNQVNLGLSAVAALDDLPLERLAYVHVAGG
                     YEHQGIWHDTHTTAAPEPVLDLLTELCARAAPPGVLLEWDDEFPSDAVLAAELGRIRAA
                     MAAGAERVR"
     gene            complement(5905..6678)
                     /locus_tag="MF672_RS05005"
                     /old_locus_tag="MF672_005005"
     CDS             complement(5905..6678)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR04222.1"
                     /locus_tag="MF672_RS05005"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MF672_005005"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_242376471.1"
                     /transl_table=11
                     /translation="MDLVLLGSAAVVAAFVLRTALELRGEHAAALAAGAGARPRPLGPY
                     ELAYVAGGARRVADTALAVLAGAGHVRVARGGTVHRVHAPASGRDPVEEAVLGVLASRS
                     GLPVTALRVETGRTLAMDALARRLAGAGLVLAPGAFERAARLAGRLRWAAVVVLAGEVL
                     TLVTIVVQGVRRSAVAALAVLAATGVFALVAAGRQRRAARATVTGAGLESLEAARAGHP
                     RGTAEEAYAVALYGLGAVRDPALQVELAAGDRGGG"
     gene            complement(6787..7770)
                     /locus_tag="MF672_RS05010"
                     /old_locus_tag="MF672_005010"
     CDS             complement(6787..7770)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR04222.1"
                     /locus_tag="MF672_RS05010"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MF672_005010"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_242376470.1"
                     /transl_table=11
                     /translation="MGFVLLVAALAVVVLVNVAGGRVEEARRRVAEAPPGGGGHFLGPY
                     ELAYLAGGPRRAINTALAVLATAGAVRVSRGSRVTAVHGARPSPVPIEQAALDALASRP
                     GGYRAAELRRELESQPALAALAAELEARGLLVPRGAFATAWRRRDQLAAVTWLAAGFVA
                     LVVTLKVTGALSGDEVFGGTVLFGVFAVIVGFGSCRKHGRRLRNVVTREGREVLRAARA
                     HHPRGVRDPGSLALAVGVPVALYGLADAGDPALCDELAAGDPGGDCAGACGTYSGGDGP
                     SYGDSYGGSDFGGSDSGGSSGCGSGSDGGSSCGGGGCGGGCGGGGD"
     gene            complement(7846..9639)
                     /locus_tag="MF672_RS05015"
                     /old_locus_tag="MF672_005015"
     CDS             complement(7846..9639)
                     /EC_number="4.1.1.32"
                     /GO_function="GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA]"
                     /GO_function="GO:0005525 - GTP binding [Evidence IEA]"
                     /GO_function="GO:0017076 - purine nucleotide binding
                     [Evidence IEA]"
                     /GO_process="GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133322.1"
                     /locus_tag="MF672_RS05015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MF672_005015"
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="WP_242376469.1"
                     /transl_table=11
                     /translation="MSLTVEVPAPTSNSELAAWVKEIAALTEPDRIEWCDGSEEEWTRL
                     TNLLVEQGTFIRLAARPNSFYAKSDPSDVARVEDRTFICSERPEDAGPTNNWIAPAEMR
                     RTFGELFKGCMRGRTMYIVPFCMGPLGGEISQVGVEITDSPYVAVSMRIMTRMGERALR
                     LIEERGDFVKCVHSVGAPLEPGQDDVPWPCSSTKYISHFPETREIWSYGSGYGGNALLG
                     KKCYALRIASVMARDEGWLAEHMLILKLTPPSGETRYIAAAFPSACGKTNLAMLQPTIP
                     GWKVETIGDDIAWMRFGEDGRLYAINPEAGFFGVAPGTGEVTNANAIRTLWGNSIFTNV
                     ALTDDGDVWWEGLTDEPPAHLTDWKGRDWTPDSDEPAAHPNARFTTPAAQCPTIAPEWQ
                     DPKGVPISAILFGGRRATAVPLVTESLSWEHGVFLGANVASEKTAAAEGKVGELRHDPF
                     AMLPFCGYNMGDYFGHWLEIGRREGAVLPRVYYVNWFRKDAGGRFLWPGFGENARVLKW
                     IVDRLNGEAKAVPTPIGLLPDHLDTEGLDLPEDDLRTLLSVDREIWREEAKLIPAHFEK
                     FGEHLPKELWQEYHALLDRLG"
     misc_feature    9704..9719
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: weak, score: 20.63"
                     /tool="antismash"
     misc_feature    complement(9756..9774)
                     /note="TFBS match to DmdR1, Iron(II)-dependent repressor,
                     confidence: weak, score: 22.41"
                     /tool="antismash"
     misc_feature    9782..9795
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.32"
                     /tool="antismash"
     misc_feature    9783..9797
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 14.69"
                     /tool="antismash"
     misc_feature    complement(9785..9802)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 18.54"
                     /tool="antismash"
     misc_feature    9788..9803
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 17.49"
                     /tool="antismash"
     misc_feature    complement(9805..9820)
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: medium, score: 22.4"
                     /tool="antismash"
     misc_feature    complement(9824..9839)
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: medium, score: 22.39"
                     /tool="antismash"
     misc_feature    complement(9846..9861)
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: medium, score: 21.47"
                     /tool="antismash"
     gene            complement(9887..10411)
                     /locus_tag="MF672_RS05020"
                     /old_locus_tag="MF672_005020"
     CDS             complement(9887..10411)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020545144.1"
                     /locus_tag="MF672_RS05020"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MF672_005020"
                     /product="hypothetical protein"
                     /protein_id="WP_302893160.1"
                     /transl_table=11
                     /translation="MATDDDVTSEVRGWFTGRLPEGWFTGAPQIIRDREEIAVIGALPD
                     PPGDTPDNERAALVEGLARRFREETRERRIEIAREAERRFRQKVSWGVSCGGLTVMFTT
                     LSVPVMTRLRQSERQVLDTLVAAGVARSRSDALAWCVRLVGRNADTWLAELRDALQHVD
                     RVRAAGPDVNS"
     misc_feature    10411..10424
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 13.79"
                     /tool="antismash"
     misc_feature    complement(10413..10426)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 13.73"
                     /tool="antismash"
     misc_feature    10535..10550
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: weak, score: 20.55"
                     /tool="antismash"
     misc_feature    complement(10684..10696)
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 19.46"
                     /tool="antismash"
ORIGIN
        1 cgtcggcaac gcgctcaagc acggcggccc cccggtcgtg gtcaacgcca ggagcacgga
       61 gaacgggctg gaggtcaagg tgcgcgacca cggcaggggc atcccgcgcg aggcgctgcc
      121 gcacgtcttc gaccgcttct tcaaggccgg cgcgggccgg gcccgcagcc agggcagcgg
      181 cctcggcctg gccatcgccc gcgcgaacgt gcacctgcac ggcggcacca tctccgtgcg
      241 cgcccaggac cccgggaccc tcttcacggt ctggctgccg cactcatgag agccgtcctc
      301 gccctcctcg ccctcctcgc cccgctggcc gccgccgcct gcggcatctc gcccaccgac
      361 gtgcaggacc ggggcaacgc gcccaccatc aggatcccgc cgccgtccaa gacgatctac
      421 ctgatccggg acggcaagct cgcgctggag ccggccgacg tcggcgacga caccgtggag
      481 agcctgctcg gcgcgctgtt cgacgcctcc tcgcagccgc tcggcgaccg ggagacggcg
      541 ctgcgcgggt tcacgtacct gcggaccaag gactcgctca acccagtgca gcgcgacgag
      601 atgcagctcc cgcgcacgct gacgctgacg gtctacatcc ggggcgaggg cacgctgagc
      661 gacctcgcca aggcgcagat cgtctgcacg gcgcagcagg acgcggcgta cgagcaggtc
      721 aagatcgtcc gggacaacga gaaccgcccc ccgaaggacg aggggcggta cacctgcggc
      781 gatctgcgcg gcgctacatc gtgatgtcct ccagcatctc ggtgaccagc gcggcgatcg
      841 gcgagcgctc ggagcgggtc agcgtcacgt gcgcgaacag cggatggccc ttcagcttct
      901 ccaccaccgc caccacgccg tcgtgccggc cgacccgcag gttgtcgcgc tgggcgatgt
      961 cgtgggtgag cacgacccgc gagttggcgc cgatccggct cagcaccgtc agcagcaccc
     1021 cgcgctccag cgactgggcc tcgtccacga tcacgaacgc gtcgtgcagc gagcggccgc
     1081 ggatgtgcgt cagcgggagc acctccagca tgcccctgtc gagcacctcc tcgatcacct
     1141 cgggcgtggt gaccgcgccc agcgtgtcgt agaccgcctg cgcccacggg cccatcttct
     1201 cgccctcggt gcccggcagg tagccgagct cctggccgcc gacggcgtag agcgggcgga
     1261 agacgaccac cttgcggtgc tggcggcgct ccaggacggc ctccagcccc gcgcagagcg
     1321 ccagcgccga cttgccggtg ccggcccggc cgcccagcga gatgatgccg atctccgggt
     1381 ccatcagcag gtcgagcgcg atgcgctgct ccgcggagcg gccgtgcagg ccgaacacct
     1441 cgcggtcgcc gcgcaccagg cgcaccgact tgtccggcag cacccggccg agcgccgagc
     1501 ccctggccga cagcagccgc agcccggtgt gcgtgggcag ctccctggcc tcctcgatgt
     1561 cggccgtgcc gtgctcgaag agcgtctcga cgtcctcggc ggtgacctgg atctcggcca
     1621 tcccggtcca gcccgactcg tgcaccagct cggcgcggta ctcctcggcg gtcaggccga
     1681 tggaggccgc cttgacccgc atgggcaggt ccttggagac cagcacgacg tcgcagccct
     1741 cggcggccag gtgacgggcc accgtgagga tgcgcgtgtc gttgtcgccg aggcggaacc
     1801 cgacgggcag gatggaagga tcgctgtgat tgagctcaac cctgagcgtg ccgtcccccg
     1861 tcggcgtcgg catgggctcg tcgagcctgc cgtgcttgat ccgcagatcg tcgagataac
     1921 gcagggcctg cctggcgaaa taaccgagct ctggatggtg tcgcttgcct tccagctcgg
     1981 tgatcacgac gatcggcaga accacgtcgt gctcggcgaa gcgggtcagg gccgccgggt
     2041 cggccaggag gaccgacgtg tcgagcacgt aggtacgctt ggccggctgg gtcgtagggt
     2101 tgctcgagga cactgccaca cgttctcccc cgggcgcccc acgcgcgggc gccctgcaga
     2161 cgaggcggga atcggaccgg acccgcgttc cccgaccgtg acgtgccggt ggtgccgggt
     2221 tccggccccc ctcggcaagt catcgtgcaa aggcgggccc tctccggagt gtccgacctg
     2281 tggccggaca ctttcaacgt tacgtcctcg atacccgaat gtcgtgtcaa gaaccgtaac
     2341 gtcgatgtct cgcagcgtaa tcgcgcagcg cccgcaggaa atccaccctg cggaagtcgg
     2401 gccagtaggc ctcgtggaag tagaactcgg aatgagcgct ctgccagagc atgaagcccg
     2461 acagacgctg ctcgcccgag gtccggatga ggaggtccgg gtcgggctgg ccgcgagtgt
     2521 agagatgctc ggcgatgtgc tcgacatcga gtacctcgac gaggtcctcg atgctcgccc
     2581 ccttgctcgc ttgctcctgg agcagtgagc gcaccgcatc ggcaatctca cgtcttcctc
     2641 catacccaac tgcaacgttc acaatcaggc ctggacggtg tgatgaagct tcttttgcat
     2701 ttttcaggac attcgccgtg cgggcgggga gcagatcgag cgccccgacg gggatgatcc
     2761 gccagccctc gtccaccagc tcgcgcgtca cgtcctcgat gatgcgcaga agcggctcca
     2821 gctcctcacg cggccggttg aggttgtcgt tggagagcat ccacacggtg acgacctcga
     2881 cgcccgtctc ccggcaccag ccgagcagct cggagatctt gtcggcgccc ttgcggtgcc
     2941 cggtggagac gtcgctcatg cccatggcgc gcgcccagcg gcggttgccg tcgatgatga
     3001 ccccgacatg ccgggggatg ctgtcccggg acagcttggc ctccagccgg cgctcgtaca
     3061 gccggtagac caggtcgcgc aggcccacgg agtgtcctcc ccagcggtat gccttcgtca
     3121 atgacccaac aggcaattat caccgcgttt cgcccgtgcc tgctccctct atcgcattcc
     3181 tcgccagatc tccgccttcc agacatcctt cggcctcccg cacgagaccg cgcacgaacg
     3241 acgacagctc ggcggaggtc agcaccgccc gcaggccgac gcccgtggcg ccccacgcct
     3301 ccacgtaggc gcgcctggtc acccgccacc ggccgccctc ccacagcggg atcgtgcgca
     3361 ggcggcagct cagctcgccg cccgccgggc ccgacctgct gcggtagcgg aaagagaaca
     3421 gctcgccgtc cggctcgtcg ccggggaagc ggtcgtcggg gtcggcccag gacggggccg
     3481 cgccctcgcg ggcctcgtcc acgccgcgcg ccaggcggct cagcagccag ccgcagcgct
     3541 cgcccacgct gccgtacggg tcgccgtcac ggtgcagctc cagcgtcacc tcgtacgggg
     3601 cgccgacgct gtcgcggcac gccaccggcg tgacgctcaa gtaggagccg tcgaggcagc
     3661 gcagtccccc catgcaccct gcgtttcccg ggcgtcaccc gctaaaccca ggcgtgcgag
     3721 cgccatcccg gcggctggtg ccactccgtc cagccgtgcc ccgagcggcc gtcctcggcc
     3781 tcgaaccggc acagcgcgcg cgggaacgcc gccaccgcgt tctccggcga caccagcgtc
     3841 accggcgcga acgccaccgg ccgcgccgtc accgcgaggc cggggaagcg cagcacggtc
     3901 tccgccggcc ggtcgccgtc gaacgtggtg gccgccgcga agccgccggc gtgcgcgagc
     3961 tcgccgcccg gcggcacggt gaacgccggc cacggcaggt cgaggccgag gttggcgcgc
     4021 atgccgtgca ggaaggtgcc gtcggacagc cgggcgctgc tccacatcca ggagatcgac
     4081 caccagtcgc ggacgcccca gctgtggtcg cgctcgccgt ggccgcggaa ggggcggccg
     4141 tcgatcgtgc cggtgacctc gcaggggatc tcgtagcgcg gggtcaggga gtagccgtag
     4201 acgccgcccg ccgtgcgcca ctccaggtcg agcgagagcg cgtcggagac cagccggacc
     4261 cgggccgcgc cgaacggctc gccggcctgc tgggacgcct cgtagcccgc gccccgcacg
     4321 gtcagggcgg ggtcgccgcc ggcggggagc ggcgcgtcgt ggtcgatcac ggacgcggcc
     4381 aggccgccgg gggtgaccag gcaggcccag taccaggcgc gcttccagtt ggggtagagg
     4441 cccagccgga cgtagccggc gagctcgccg tcgccggcga cgaagtcgaa atagtaggac
     4501 tcgttccaca gcggctcgcc gccgggctcg tggacgtgct cgtccagtgg ccactcgccc
     4561 atgttgacga tgcgcatgtc gcctcccggg cgcagcttac ggcacgagcc tggaccacca
     4621 gcgggacctg cggcggggcc ggtcctccgc cgccatcagg ccgcgctcgc gcagccaggc
     4681 ggcgaagtcg tcggcgtcgg cgcggtagcc gggcggcggc tcggtgcgcg cgcgggcgta
     4741 cgcggcgaac tcggcggcca gcttcggccc cgccgccacg gcggtgtcgg gacggatgcg
     4801 cgccacgatg gcccgccgct tggcgaccag gctggccgcc tgggcgcgca tccgggcctg
     4861 gtcgaagccc tcgggcacct cgcccgccgc gaccagcgcc cgcaccaccc gggcctgcgc
     4921 ctccgccagc cgggcctgcg cctccgccag tcgctcctcc tcgggccgct cctcctcggg
     4981 ccgctcctcc tcgggcgggc tcatcggacc cgctccgcgc ccgcggccat cgcggccctg
     5041 atgcgcccca gctcggcggc gagaaccgcg tcgctcggga actcgtcgtc ccactccagc
     5101 agcacgccgg gcggggccgc gcgggcgcac agctcggtca gcaggtcgag gaccggctcg
     5161 ggcgcggccg tggtgtgggt gtcgtgccag atgccctggt gctcgtaacc gccggcgacg
     5221 tgcacgtacg ccaggcgctc cagcggcagg tcgtcgagcg cggccaccgc cgacaggccc
     5281 aggttgacct ggttggtgta gaggttggcc acgtcgacca gcagccgcac ccccgtccgc
     5341 tcgaccagct cggcgaggaa gcggccctcg gtcagctcgt cgtccggcca gccgaagatg
     5401 gcggccacgt tctccagcgc cagcgggacg ggcagcgcgt cctgggcccg gcgcacgttc
     5461 tcggtcacca cccgcagcga ctcccgggtg cgcgggatcg gcagcaggtg ccccgcctcc
     5521 aaaccgccgc ccctgacgaa cgccaggtgc tcgctgacca gcggcgcgtc cagcgcctcc
     5581 gcgcaggcgg ccaggtgcgc cagccggccg ggggacggcg gctcggcccc gccgatcccg
     5641 agcgacaccc cgtgcgggat caccggcacg cccttggccc gcagcagccg cagcgactcc
     5701 ggcaggtcgc cgggccgcag gttctcggcg atggcctcga cgaagtccac gcccgccaga
     5761 cgctcgacgg tgaggtccag ctccgaccgc cagccgatgc cgacgcccag ctccgtccgc
     5821 ccgttcccgt cgtcccgctc ccccgtcatg ccgccgctcc tgtctccccg cacctcggtt
     5881 ctccttcccg tgacgatccc gtcgtcagcc gccgccccgg tcgcccgccg ccagctccac
     5941 ctggagagcg gggtcgcgga ccgcgccgag gccgtacagc gcgaccgcgt acgcctcctc
     6001 ggccgtcccc cgcggatgcc cggcccgcgc cgcctccagc gactccaggc ccgcgccggt
     6061 caccgtggcg cgcgcggccc gccgctgccg cccggccgcg accagcgcga acacccccgt
     6121 cgccgcgagc acggccagtg cggccaccgc cgaccggcgc acgccctgga ccacgatcgt
     6181 caccagcgtc agcacctcgc cggcgagcac caccaccgcg gcccaccgca gccgcccggc
     6241 cagccgggcg gcccgctcga aggcgccggg ggcgagcacc aggcccgccc cggcgagccg
     6301 gcgggcgagc gcgtccatgg cgagggtgcg gccggtctcg acgcggagcg cggtcaccgg
     6361 cagccccgag cgggaggcga gcacgcccag caccgcctcc tccaccgggt cgcgccccga
     6421 ggcaggggcg tgcacgcggt gcacggtgcc gccgcgcgcc acccgtacgt gaccggcccc
     6481 ggccagcacc gcgagcgccg tgtccgccac ccgccgcgcc ccgccggcca cgtacgccag
     6541 ctcgtacggc ccgagcggcc gcggccgcgc cccggccccg gccgcgaggg cggcggcgtg
     6601 ctccccccgc agctccaggg ccgtgcggag gacgaacgcg gcgaccaccg ccgcggagcc
     6661 gagcaggacc agatccatcc gagccctccc tccgaaggca gcccgacggg ccgggtaccc
     6721 ggaagacgag tggagcgggc gggaaggttc cgcctccggg gccacacttc cgtccacgcc
     6781 gggaagtcag tcgccgcccc cgccgcagcc gccgccgcaa ccgccgccgc cgcagctcga
     6841 cccgccgtcc gacccgctcc cgcagccgga ggagccgccc gagtccgagc cgccgaagtc
     6901 cgagccgccg taggagtcgc cgtaggacgg cccgtcgccg ccgctgtacg tcccgcaggc
     6961 gccggcgcag tcgccgcccg ggtcgccggc cgccagctcg tcgcagagcg ccgggtcgcc
     7021 cgcgtccgcc agcccgtaca gcgccaccgg cacccccacg gccagcgcca gcgagcccgg
     7081 gtcgcgcacg ccgcgcggat ggtgggcgcg cgcggcccgc aacacctcgc ggccctcccg
     7141 cgtcacgacg ttccgcagcc gcctgccgtg cttgcggcag gacccgaagc cgacgatcac
     7201 ggcgaacacc ccgaacagca ccgtcccgcc gaagacctcg tcgccgctca gcgcgccggt
     7261 caccttcagc gtcaccacca gcgccacgaa ccccgccgcc agccacgtga cggccgcgag
     7321 ctggtcgcgc cgccgccacg ccgtcgcgaa cgccccgcgc ggcacgagca gcccgcgcgc
     7381 ctccagctcg gcggcgagcg cggccagggc gggctgcgac tccagctcgc gccgcagctc
     7441 ggcggcccgg tagccgccgg ggcgggaggc gagcgcgtcc agcgcggcct gctcgatcgg
     7501 caccggcgaa ggccgcgccc cgtgcacggc ggtgacccgg gagcccctgg agacgcggac
     7561 ggcgccggcc gtcgcgagga cggccagcgc cgtgttgatg gccctgcggg gcccgcccgc
     7621 caggtacgcc agctcgtacg ggcccaggaa gtgcccgccg ccgcccggcg gagcctcggc
     7681 gacgcgccgc ctggcctcct cgacccggcc gcccgccacg ttgaccagca cgaccacggc
     7741 gagcgcggcc acgagcagga cgaaccccac gatgcccccc aatgcgtcgg tacggcggca
     7801 ggcgccaagg tagggccagg ccgccgcacc gcacaagggc ggccgctacc cgagccggtc
     7861 gagcagcgcg tggtactcct gccacagctc cttcggcagg tgctcgccga acttctcgaa
     7921 gtgggccggg atcagcttgg cctcctcgcg ccagatctcg cggtccacgc tgagcagcgt
     7981 gcgcaggtcg tcctcgggca ggtccaggcc ctcggtgtcg aggtggtcgg gcagcaggcc
     8041 gatcggggtc ggcacggcct tggcctcgcc gttgagccgg tcgacgatcc acttcagcac
     8101 gcgggcgttc tcgccgaagc ccggccacag gaagcggccg ccggcgtcct tcctgaacca
     8161 gttgacgtag tagacgcgcg ggagcacggc gccctcgcgc cggccgatct ccagccagtg
     8221 gccgaagtag tcgcccatgt tgtagccgca gaacggcagc atggcgaacg ggtcgtggcg
     8281 cagctcgccg accttgccct cggccgcggc ggtcttctcg gaggcgacgt tggctccgag
     8341 gaagacgccg tgctcccagc tcagcgactc cgtgaccaga ggcacggcgg tggcgcggcg
     8401 accgccgaac aggatcgccg agatgggcac gcccttgggg tcctgccatt cgggggcgat
     8461 ggtcgggcac tgcgcggccg gggtggtgaa gcgggcgttc gggtgggcgg ccggctcgtc
     8521 gctgtccggc gtccagtcgc ggcccttcca gtcggtcagg tgcgcgggcg gctcgtcggt
     8581 caggccctcc caccacacgt cgccgtcgtc ggtgagggcg acgttggtga agatgctgtt
     8641 gccccagagc gtcctgatcg cgttggcgtt ggtgacctcg ccggtgccgg gggcgacgcc
     8701 gaagaagccg gcctccgggt tgatggcgta caggcggccg tcctcgccga agcgcatcca
     8761 ggcgatgtcg tcgccgatgg tctcgacctt ccagccgggg atcgtgggct ggagcatggc
     8821 gaggttggtc ttgccgcagg cgctcgggaa ggcggcggcg atgtagcggg tctcgccgga
     8881 gggcggggtg agcttcagga tgagcatgtg ctcggccagc cagccctcgt cgcgggccat
     8941 gacgctggcg atgcgcagcg cgtagcactt cttgccgagc agcgcgttgc cgccgtagcc
     9001 ggagccgtag gaccagatct cgcgggtctc ggggaagtgg ctgatgtact tggtggagct
     9061 gcacggccag ggcacgtcgt cctggccggg ctccagcggg gcgccgacgg agtgcacgca
     9121 cttgacgaag tcgccgcgct cctcgatgag gcgcagcgcc cgctcgccca tgcgggtcat
     9181 gatccgcatg gagacggcca cgtacgggga gtcggtgatc tcgacgccga cctgcgagat
     9241 ctcgccgccg agcgggccca tgcagaacgg gacgatgtac atggtccggc cgcgcatgca
     9301 gcccttgaac agctcgccga acgtccggcg catctccgcc ggggcgatcc agttgttggt
     9361 gggcccggcg tcctccgggc gctcggagca gatgaaggtg cggtcctcga ccctggccac
     9421 gtcgctcggg tcggacttgg cgtagaagct gttggggcgg gcggcgagcc ggatgaacgt
     9481 gccctgctcg acgaggaggt tcgtcaggcg cgtccactcc tcctcggagc cgtcgcacca
     9541 ctcgatccga tcgggctcgg tcagggctgc tatctctttc acccacgcgg ccaattcgct
     9601 attgctcgtc ggcgcgggca cttccacggt aagggacacg actgcgactc ctccactctc
     9661 tcagggccca gtcatcatta acgacgaagc ggcccgaaag ccaattggcg tagacctgtt
     9721 ggctgggccg tcgtcgcagg caggggccct ggcgattggg ggagcctacc caaccgcgga
     9781 ggatttaaaa gagaattaac attgactggt ccagtccaat tccgctggtc taaaccaatt
     9841 tccgcagtgg tttagtccat tgctccggcc gtttgccctg gacgctctag gaattgacgt
     9901 ccgggccggc ggcgcgcacg cggtcgacgt gctggagagc atcgcggagc tcggcgagcc
     9961 aggtgtcggc gttgcggccg accaggcgga cgcaccaggc gagcgcgtcg ctgcggctgc
    10021 gggccacgcc ggcggccacg agcgtgtcga ggacctgccg ctccgactgg cgcagccggg
    10081 tcatgaccgg caccgacagt gtggtgaaca tcacagtgag gccgccgcag gagacgcccc
    10141 aggacacctt ctgccggaac ctgcgctcgg cctcgcgggc gatctcgatg cgccgctcgc
    10201 gggtctcctc cctgaagcgc cgcgccagcc cttcgacgag ggcggcgcgc tcgttgtccg
    10261 gggtgtcgcc gggcgggtcg ggcagcgcgc cgatgaccgc gatctcctcg cggtccctga
    10321 tgatctgcgg ggctccggtg aaccagccct cggggagccg tccggtgaac cagccccgga
    10381 cctccgacgt cacgtcatcg tctgtagcca tgtaatcaac attacacagt tgcaggcgac
    10441 gcgcacggga gggaaggaag gtgtcgcccg gtggagatgc ctagcggacc tgcgcggcgg
    10501 cgatcgggct cacccgcacg acgccggcca ggttcctggt gtagaccttc gagatcttga
    10561 cgacgtcgcc ggtgcgcggc gcctggatca tctgcccgtt accccaataa atcgccacat
    10621 gggagatgta gtccgggttc gtggggtcgg accgccagaa gatgaggtcg cccggctgcg
    10681 cctgcgacag cggcacctgc gccccggtca cccactgctg gtgcgtcacg cgcggcatgc
    10741 gcacgcccgc ctgcgcgaag gcccactgga cgaggcccga gcagtcgaag gtgtcggggc
    10801 cctcggcgcc ccacacgtac ggccgcccca gcttggaggc ggcggcccgg agcgccacct
    10861 ggatctgctc ggc
//
