LOCUS       NZ_CP023711            20549 bp    DNA     circular CON 06-APR-2024
DEFINITION  Paenibacillus polymyxa strain C12 chromosome, complete genome.
ACCESSION   NZ_CP023711
VERSION     NZ_CP023711.1
KEYWORDS    RefSeq.
SOURCE      Paenibacillus polymyxa
  ORGANISM  Paenibacillus polymyxa
            Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae;
            Paenibacillus.
REFERENCE   1  (bases 1 to 5793823)
  AUTHORS   Sun,D., Qiu,J. and Shen,M.
  TITLE     De Novo Assembly of the Paenibacillus polymexa Strain C12 Genome
            Using PacBio Single-Molecule Sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5793823)
  AUTHORS   Sun,D., Qiu,J. and Shen,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2017) College of Biotechnology and Bioengineering,
            Zhejiang University of Technology, Chaowang Road, Hangzhou 310000,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 2.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 105.2x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022649565.1-RS_2024_04_06
            Annotation Date                   :: 04/06/2024 06:53:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 4,973
            Genes (coding)                    :: 4,899
            CDSs (with protein)               :: 4,899
            Genes (RNA)                       :: 156
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 110
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 26 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 01:37:22
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 593417
            Orig. end                         :: 613966
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP023711.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..20549
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="cyclic-lactone-autoinducer"
                     /region_number="5"
                     /rules="(TIGR04223 or AgrB)"
                     /tool="antismash"
     cand_cluster    1..20549
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(TIGR04223 or AgrB)"
                     /kind="single"
                     /product="cyclic-lactone-autoinducer"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20549
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:10549](-)"
                     /cutoff="20000"
                     /detection_rule="(TIGR04223 or AgrB)"
                     /neighbourhood="10000"
                     /product="cyclic-lactone-autoinducer"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..10549)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(TIGR04223 or AgrB)"
                     /neighbourhood="10000"
                     /product="cyclic-lactone-autoinducer"
                     /protocluster_number="1"
     misc_feature    910..924
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.99"
                     /tool="antismash"
     misc_feature    complement(1004..1018)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.88"
                     /tool="antismash"
     misc_feature    complement(1078..1093)
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 21.04"
                     /tool="antismash"
     misc_feature    2310..2325
                     /note="TFBS match to CsoR, Copper-responsive repressor,
                     confidence: weak, score: 22.48"
                     /tool="antismash"
     misc_feature    2836..2849
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: medium, score: 21.31"
                     /tool="antismash"
     misc_feature    3058..3072
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 21.3"
                     /tool="antismash"
     gene            complement(3194..4366)
                     /locus_tag="CPY53_RS02150"
                     /old_locus_tag="CPY53_02195"
     CDS             complement(3194..4366)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1244: amidohydrolase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013371417.1"
                     /locus_tag="CPY53_RS02150"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CPY53_02195"
                     /product="M20 family metallopeptidase"
                     /protein_id="WP_017427740.1"
                     /transl_table=11
                     /translation="MLEKWVQELKDGEQELIAWRRYLHQHPELSFEETNTSAFIADQLR
                     SFGIEVRTNVGGNGVLGFLEGGQPGRTIAFRADFDALPIQDEKDAPYKSTVPGVMHACG
                     HDGHTAALLGVARVLSHHRKTLKGKLVFIFQHAEEKPPGGAKFMIEDGCLDGVEAVYGI
                     HLSSEIPLGKIGLKGGPAMAAADAFSIEINGKGGHGARPHQTVDSIVIGSQIVNGLQQV
                     VSRRVDPTESAVLTIGVFQAGTAFNVIADKAKIEGTVRTFNKDIRKEVENEIRSIVKGL
                     TDAYHAGYEIDYLNGYPALVNAEAETERVRELVSRLYGADAFMDLKPAMGAEDFAYYLE
                     QRPGAFIIVGARNEDERTHFAHHHPRFDFDERALLISGHIFLALALEYLQ"
     gene            complement(4595..5104)
                     /locus_tag="CPY53_RS02155"
                     /old_locus_tag="CPY53_02200"
     CDS             complement(4595..5104)
                     /EC_number="2.3.1.-"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017427739.1"
                     /locus_tag="CPY53_RS02155"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CPY53_02200"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_017427739.1"
                     /transl_table=11
                     /translation="MGYNIKSELKLSIKSVPESQKDIIVQLMQFYLYDFTAYLRIDVTE
                     KGTFPAYPDLYQYWTSGEQKLPFLIWRQDIPVGFALVDRMSSNREADYYMAEFFVLRPY
                     RHNGVGTWAAHELFGRFNGRWKVTQMSTNRPAQSFWRKVIGSYTNGEYREQTHPVRGNI
                     SQFFNT"
     misc_feature    5088..5104
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 23.71"
                     /tool="antismash"
     gene            5214..6314
                     /gene="xerS"
                     /locus_tag="CPY53_RS02160"
                     /old_locus_tag="CPY53_02205"
     CDS             5214..6314
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /gene="xerS"
                     /gene_functions="other (smcogs) SMCOG1077: phage integrase
                     family protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019687399.1"
                     /locus_tag="CPY53_RS02160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CPY53_02205"
                     /product="tyrosine recombinase XerS"
                     /protein_id="WP_017427738.1"
                     /transl_table=11
                     /translation="MNIQKIIDRRKLDEKLPDLPWYVQQFMDYKLPDLSPSTLLEYVRD
                     YEAFFSWLRSEGLSAGETNAQVTLEELETLHMDSITGFRLFLSTKREGSNSRITISRKL
                     SSLRSLFHYLSQIAEDENFYPLLKRNIMAKVEIKRVHKPKDTAAKLKGKILEEEELLEF
                     IGYIYEGYGQDLADNKQALYSYELNKERDACIASLILNSGLRVSEIVNLNMDDLDINNK
                     LLYVYRKGNNDETFKTPVYFREQSKDDLALYLSLRHTRYRAPKKEKALFVARPNGSAEG
                     KRMTKRAIQAMIIKYAKRFGKPYLTVHKLRHSFATDYYLQNDIYKTKEQLGHASTETTE
                     VYAHLTDKTMSQAIERRIETQTDSAN"
     gene            complement(6323..6874)
                     /gene="mobB"
                     /locus_tag="CPY53_RS02165"
                     /old_locus_tag="CPY53_02210"
     CDS             complement(6323..6874)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /GO_function="GO:0005525 - GTP binding [Evidence IEA]"
                     /GO_process="GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="mobB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013371420.1"
                     /locus_tag="CPY53_RS02165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CPY53_02210"
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="WP_016820736.1"
                     /transl_table=11
                     /translation="MKTKYPFVCQIVGFKNTGKTTFLGGLIRYAQQSGLRVAVIKHDGH
                     EFEMDHEGTDTYKHRQAGAEGVAIVSAGRTAILEEHSRPVIGLIRHFQAYDCVLLEGFK
                     QEKYPKLIMVREQSDAQLVQELTNVQGVVVWEYHMDWAQDYYSDEQTVQIFPADASEPA
                     GCWLLEQIQRHKMGFQTEQG"
     gene            complement(6871..8148)
                     /gene="glp"
                     /locus_tag="CPY53_RS02170"
                     /old_locus_tag="CPY53_02215"
     CDS             complement(6871..8148)
                     /GO_component="GO:1990586 - divisome complex [Evidence
                     IEA]"
                     /GO_function="GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="glp"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013371421.1"
                     /locus_tag="CPY53_RS02170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CPY53_02215"
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="WP_017427737.1"
                     /transl_table=11
                     /translation="MKQTPLNTEKFQRKAVKVEEAQSRIASYVQQGEREDVGLEQAHGR
                     YLAMDLTAPHPYPRFRRSGMDGYAILASDTEVCSTGQEIWLHVIDEIPCGTVSDKRVET
                     GMAVRIMTGAQLPEGADTVVMLEATQLREENGQTYVGLKKRMEVGKNVTQIGHEIKEGQ
                     LLLSKGTCVGAGHVSVLATFGVHRVPVYRKPRVAVFSTGSELLAVDEPLQPGKIRNSNT
                     YMLASQIREAGGEPFILQAIHDDLNLARASVREAIAAYDIVVTSGGVSVGDFDIMGDLV
                     RQEDVNMLFNKVTMRPGSVTTAAVIDGKLLFALSGNPGACFVGCELFVRPTIQMMLSSA
                     HPYLQTWTAKLGADYTKVNNYTRFVRSSLEIRSGQVYAIPARVDESSAMVTIKDSDCLI
                     VVPPTTEGLHEGEEVHVLVLQGGQVT"
     gene            complement(8344..8583)
                     /locus_tag="CPY53_RS02175"
                     /old_locus_tag="CPY53_02220"
     CDS             complement(8344..8583)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016820734.1"
                     /locus_tag="CPY53_RS02175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CPY53_02220"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_016820734.1"
                     /transl_table=11
                     /translation="MVADKNENVTCTSFYTIKMARLASGYSELIACNILGMSMNELKHI
                     EEDSSDLTIARAQQMSSLYEVSIDSIYFGKNNIT"
     gene            8672..8887
                     /locus_tag="CPY53_RS02180"
                     /old_locus_tag="CPY53_02225"
     CDS             8672..8887
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /locus_tag="CPY53_RS02180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CPY53_02225"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_016820733.1"
                     /transl_table=11
                     /translation="MQKLTRGKCLLKEELKSHGRTQSWLARRTGYSRQQISNWIAGRDK
                     MSFEAAVLISRILECKAEDLYEWELA"
     gene            complement(9157..9891)
                     /locus_tag="CPY53_RS02185"
                     /old_locus_tag="CPY53_02230"
     CDS             complement(9157..9891)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016820732.1"
                     /locus_tag="CPY53_RS02185"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CPY53_02230"
                     /product="hypothetical protein"
                     /protein_id="WP_016820732.1"
                     /transl_table=11
                     /translation="MEEGKRILATPLLDDNSLGDCSFFCENHLVAIELWKPKSNYHIPL
                     FHTSHGRFTVPTTLHECSVGLPTFCNLDGSNLVNITQVDKIVTGDYGGGQVVFKNQDIK
                     ESINSANLSRWKQIYENAMNADREIRYIFGSEIKVVGKAAVSGFFKVGNMLSVDMWEPK
                     KNYYVPRFHSGDRSYSIGLTAQACREAFPYLYPAYKDTLINLELVCEIESNAFGGLVRF
                     EGSDFTCSMSHNKLKALKKLWK"
     gene            complement(9897..10028)
                     /locus_tag="CPY53_RS25565"
     CDS             complement(9897..10028)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017427735.1"
                     /locus_tag="CPY53_RS25565"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_277933267.1"
                     /transl_table=11
                     /translation="MDKKLARALSISLGNYANESTKKEKTIFGAKPVLAELKKTEKN"
     gene            complement(10001..10549)
                     /locus_tag="CPY53_RS02190"
                     /old_locus_tag="CPY53_02235"
     CDS             complement(10001..10549)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_process="GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     cyclic-lactone-autoinducer: AgrB"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /locus_tag="CPY53_RS02190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CPY53_02235"
                     /product="accessory gene regulator B family protein"
                     /protein_id="WP_242210984.1"
                     /sec_met_domain="AgrB (E-value: 2.2e-20, bitscore: 71.0,
                     seeds: 181, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLETLSRHIASEIKKADPDGPTSVEVMEYVIGVKITEISAVLMVA
                     VMAWITGHFWGALLALATIMFVRRFSGGVHFSNLTLCVCFTTAICVTIPFVSLSLSTIL
                     IINACSLLMFVVYAPNHFIYINKTKHHKYYKTICILVCILNIFIQSHIICLSLIIQAFS
                     ILPLWKGGERKWIRNWREL"
     gene            complement(10500..11213)
                     /locus_tag="CPY53_RS02195"
                     /old_locus_tag="CPY53_02240"
     CDS             complement(10500..11213)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016820730.1"
                     /locus_tag="CPY53_RS02195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CPY53_02240"
                     /product="hypothetical protein"
                     /protein_id="WP_016820730.1"
                     /transl_table=11
                     /translation="MLSAFLYIILGFFDALAAVTLVLKLYMLPVWENRRKIIFYAMGIA
                     LFSFLMREVIGMPKFDFPLQYLLMVLFLRFGLGIKTYLAAFSAGSGLTAYINLQLFVYI
                     FANFFGVAQPGVISDTAGSSIYVIQLASIFTAYFISFVMGKYNFGFSFIIQPPHDFLKG
                     ENYLSPLNKMFIIGSLISFVTILVTLYMLYNSNTIGLLSISLLTFGISYFLSEKGDYED
                     ARNAIEAHRIRNKES"
     misc_feature    11334..11348
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 21.08"
                     /tool="antismash"
     gene            11480..11713
                     /locus_tag="CPY53_RS02200"
                     /old_locus_tag="CPY53_02245"
     CDS             11480..11713
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016820729.1"
                     /locus_tag="CPY53_RS02200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CPY53_02245"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_016820729.1"
                     /transl_table=11
                     /translation="MALSDNFKKLRIKYHLTQQELAEIAGVTNKAISAWESGLSEPRMG
                     AIEKIARHLHIKKSNIIEEDGIEHISPDRRSK"
     misc_feature    complement(11490..11502)
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 19.31"
                     /tool="antismash"
     gene            complement(11847..12602)
                     /locus_tag="CPY53_RS02205"
                     /old_locus_tag="CPY53_02250"
     CDS             complement(11847..12602)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013310483.1"
                     /locus_tag="CPY53_RS02205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CPY53_02250"
                     /product="DUF4247 domain-containing protein"
                     /protein_id="WP_016820728.1"
                     /transl_table=11
                     /translation="MSKRTWQYALKIIVALSLFVSVLSGCGAPNVKDTYPLESVSGSGN
                     STSYVYRAAGKTVPEVAEELKEQRTPEQISPQSNERMFLVYSDEWYHLQQDPAKKEDTL
                     IEIDSKKYVEQNYSSSFLKGYLTATVLDALFDSLRGSGSYRGYSSRDVYRPSQGQYRAP
                     TAQEKKAIPPITVERKGSVTRRGKNSDSSVGSSGSLFDRGSSSSPSRGSISRDRGSSGG
                     SSIFDSPRKSYSKPKTRSGSGKISRRSRR"
     gene            complement(12616..13122)
                     /locus_tag="CPY53_RS02210"
                     /old_locus_tag="CPY53_02255"
     CDS             complement(12616..13122)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010348250.1"
                     /locus_tag="CPY53_RS02210"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CPY53_02255"
                     /product="DUF4178 domain-containing protein"
                     /protein_id="WP_017427734.1"
                     /transl_table=11
                     /translation="MSVWKRISNLFAKPEPPKAEKSMLHLNPGDICEVSLVTYEVVGRV
                     HNRARNAVVLTLQDGNQVSYLHIEERETLQFGLYQAIDGRLDNPEEVPTILELDDTTYH
                     LEEQYFGHVTVMGRAPFRQGGEQHVWQYQSDDYHLLRVEWQDGRFMLYEGEEVITGDVK
                     VIRAS"
     misc_feature    13099..13113
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: strong, score: 24.68"
                     /tool="antismash"
     gene            complement(13147..13554)
                     /locus_tag="CPY53_RS02215"
                     /old_locus_tag="CPY53_02260"
     CDS             complement(13147..13554)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016820726.1"
                     /locus_tag="CPY53_RS02215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CPY53_02260"
                     /product="DUF350 domain-containing protein"
                     /protein_id="WP_013371429.1"
                     /transl_table=11
                     /translation="MHFLETMKAMLVWTGAGAILLFVLMYVDSLFTKYKDFAEVKAGNM
                     AVTVRMIMKLIAQGYILSGSIATANNLLEALVYSVIAFIILLVLEVIVRLLLRYWAQFD
                     LDKGTQEGKIGFGLFSGALHLVGALIITACF"
     gene            complement(13684..14352)
                     /locus_tag="CPY53_RS02220"
                     /old_locus_tag="CPY53_02265"
     CDS             complement(13684..14352)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007432515.1"
                     /locus_tag="CPY53_RS02220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CPY53_02265"
                     /product="PspA/IM30 family protein"
                     /protein_id="WP_013371430.1"
                     /transl_table=11
                     /translation="MSIFKRLRDLTMSNINAIIDKAEDPVKMTDQYIRDMQEDLEDAEK
                     AVAAQIAIEKRFKQQYEEQAALVKKREEQAHTAAKAQNVDLARRALEEKKSAEQKMNEF
                     QAGYEQNKAAADNLRGKLDEMRRQLTEMKNKRETLVARYNAAKAQTEINKAMNGFSSDT
                     ATAGLKRMEEKMLQAEARAEASNEMASKEKSLDEEFKELDKKSAVDDELAALLKQYENK
                     "
     gene            complement(14439..15461)
                     /locus_tag="CPY53_RS02225"
                     /old_locus_tag="CPY53_02270"
     CDS             complement(14439..15461)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1217: NADH:flavin oxidoreductase/NADH oxidase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013371431.1"
                     /locus_tag="CPY53_RS02225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CPY53_02270"
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="WP_016820725.1"
                     /transl_table=11
                     /translation="MTDLFTPYQLKGLSLRNRIVMPPMCQYSVEAKDGKPTDWHYVHYV
                     SRAVGGTGLIIVEMTAVHPDGRITDRDLGIWSDEHIPAYRKMVEEVHKYGAKIAIQLGH
                     AGRKAEDAEPPVGPSSIPFSERFKEPHALTRAEVKELVLAYKEGARRAVEAGFDTVEIH
                     GAHGYLIHQFHSPLTNTREDEYGQDLSLFGVEVIRAVKEVLPADMPVLMRISAKEYVQD
                     GYDVEYIAAIGERYRDAGVDIFHISSGGEGPIGSSGGPSARAGYQVDLAHTIREKLNVP
                     VIAVGLLDDYEDARRVVQHNEADLVAIGRAMLRDPYWALHASRALKVQADIPEPYLRGF
                     "
     gene            15765..16571
                     /locus_tag="CPY53_RS02230"
                     /old_locus_tag="CPY53_02275"
     CDS             15765..16571
                     /EC_number="3.-.-.-"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1235: polysaccharide deacetylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016820724.1"
                     /locus_tag="CPY53_RS02230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CPY53_02275"
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="WP_016820724.1"
                     /transl_table=11
                     /translation="MRYRGVLPCLALLLAFSSFPVHADTPPNIGTPHPKEATVATSVYQ
                     SKDRATPTLAMLRKKHADIFKMCGPHTRQIALTFDDVPDSRYTPQVLDVLHANGIKATF
                     FIVGHRAEKHPGIIRRIIREGHAIGNHSYTHPDFRKATPERFRHQITKTERILEASAGF
                     RPRLIRPPYGEITEPQIQWARRHGYMIVNWNVDSLDWKGLSKTQIQRNVIPATRPGSII
                     LMHAGGGQSSNLQGTVQALPGLIHSLKAKGYKFVTVPRLLHTSERK"
     gene            complement(16771..17439)
                     /locus_tag="CPY53_RS02235"
                     /old_locus_tag="CPY53_02280"
     CDS             complement(16771..17439)
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_function="GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /GO_process="GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019687404.1"
                     /locus_tag="CPY53_RS02235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CPY53_02280"
                     /product="3D domain-containing protein"
                     /protein_id="WP_019687404.1"
                     /transl_table=11
                     /translation="MKHLKHIGKTMKFAVLAAAIAGLAQTAGVANAASLHTAKEGDTFF
                     LLAQRYGVSVDDLTKANPDIASNNIYAGLQIKVPDEKSVKAASDSKPQLLTEKAPANER
                     PTIEAWGKTFNYSKVVDVKATAYSAAADENGKWGAVDYYGNPLKLGTIAVDPKVIPMGT
                     KVLVTGYSHPGLPDHSFVAVARDQGSAITGKRIDIFIPGNKSFVNDFGFQNVKLYVIDK
                     "
     regulatory      complement(17446..17615)
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /regulatory_class="riboswitch"
     gene            complement(17859..18581)
                     /locus_tag="CPY53_RS02240"
                     /old_locus_tag="CPY53_02285"
     CDS             complement(17859..18581)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013371435.1"
                     /locus_tag="CPY53_RS02240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CPY53_02285"
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="WP_013371435.1"
                     /transl_table=11
                     /translation="MITVKQLRKSFGKNEILKGIDIDIAKGEVVVIIGPSGSGKSTFLR
                     CMNLLEQPTEGEILFEGEPITARGHNINATREKMGMVFQHFNLFPHKTILQNLTLAPTQ
                     VKKQSGKEAEKIALELLRTVGLEDKKNTYPNQLSGGQKQRIAIARALAMQPHVMLFDEP
                     TSALDPEMVGEVLDVMKKLAEGGMTMVIVTHEMGFAREVGDRIIFMDNGQIIEQGTPEQ
                     VFGTPSHDRTRDFLAKVL"
     gene            complement(18568..20031)
                     /locus_tag="CPY53_RS02245"
                     /old_locus_tag="CPY53_02290"
     CDS             complement(18568..20031)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1091: glutamine-binding lipoprotein glnH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013371436.1"
                     /locus_tag="CPY53_RS02245"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the opines
                     (in Agrobacterium) octopine and nopaline, etc.; The
                     N-terminal region of this protein, as described by
                     TIGR01726, is a three transmembrane segment that identifies
                     a subfamily of ABC transporter permease subunits, which
                     specificities that include histidine, arginine, glutamine,
                     glutamate, L-cystine (sic), the opines (in Agrobacterium)
                     octopine and nopaline, etc; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /old_locus_tag="CPY53_02290"
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="WP_242210985.1"
                     /transl_table=11
                     /translation="MKKAYRFTALVLTAVILLLAGLPMLTAYGSEGKPQKKLVVGMSAD
                     FPPYEFHKVINGQDTIVGFDVEIAKEIAKDLDAEMVPEDMSFDSLLPALQSGRVDLVLS
                     GMTPTDERRKSIDFSDVYYRSKQVIMVRPEDKDKYSTMAALKGEKLGAQKGSIQEEIAQ
                     KVPNASVTSLDKISDLVLQLRTKRINAVVIEDTVAKGYTLDGTVTLAKAVPEDEGAETA
                     VGIRKGNAELLASVNKTLARLKADGSIEKFSTEASALFADRQVSSNSIVDIFLKYRSFY
                     ATGIGYTLLLSALGVLFGFIIGLIISLLRMSGIRIIEWLGTAYVEILRGTPMLVQLMII
                     HYGVALTLGVNFTPLQSGIITLSLNSSAYLAEIFRAGIQAVDRGQMEAARSLGMGRGKA
                     MRHVILPQAFKSILPAIGNEFITIIKESSIISTIGMVDIMYQAQVIKNITYEGLSPFVI
                     AAAIYFVMTFSLSKLLGLWERKLRASDHR"
     misc_feature    complement(20092..20105)
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: strong, score: 19.49"
                     /tool="antismash"
ORIGIN
        1 gctagggaaa cgggtgagct tcctgtattg gttattttaa acttggaact gatcgtatta
       61 cttgttgcag atacattccc attgaaggat tgcaccttca gagcaccctc gcctgttccc
      121 ggctcacttg gcgtatcccc tttcaccgta atcgtgaaca acgcatcatt gcctgtacta
      181 aaatggaagt tgagcgttgt ctctccttta ggaagtttag ataaatactt gttggtaaga
      241 ataactgttt cttcactaac cgtataatct tcacctttgt tcagtgccac actcccctgc
      301 ttgatcgctt ctagtgtatt accgttaaga gtaagcttga cagtaatatc gatattactg
      361 ttagccactt cataggttgc agccgtcggc gtaatacttg cttgcccggt agatggctgg
      421 tcaccaccca cacgatctgc caccagaatg ccgcggccat tcgttcccag ataagcgcgt
      481 ccgtaaaccc gagggtcccc aataatggtg gtcactcgag catattgatg cttgtcatca
      541 ttaatgcgca cccagtttgc tccagcatcg tccgaacgat aaaatccacg agttccgtca
      601 atcttaccaa cgataaacac tgctgcattg gtgcgattgg gtgctgcttt gccaaagcct
      661 acaaaatccg cctcctgcac atgatcaagc ttggtgaatg taatacctga atccttggaa
      721 tgccataagc cgtatggccc tccgtcctcg gagcctccag caaaccacac atctccttcc
      781 gctcctcgta cagcaccaat atctgcattc cctacacttg gcaaaccagt aactgtggat
      841 ggtgtgaagg atgcgcctcc atcaacactt acatatattt tcccagcagc aaacgcgtag
      901 tatttgttag gatttactcg gtcagaggcg atctttgcat tagctggaac tccggtgctt
      961 gctgtccatg agtttcctcc ggttttggaa taaaatacac ctttgtccga tgtactccac
     1021 agcaatccat taccatttgc cgctacagcg atgctgcctc ctcctgctgt accggaaggc
     1081 tcgctattag ctttgtacca gttcgctccg ccatcattgg ataatccgat agatttggcg
     1141 tttggatctt ttttatagtc cgctttacct acccgagcca taaaagaagg gcttaactcg
     1201 gcataatcaa tgctttcgct gcttgtaaag gttggattat ccagcatttt agcaggagcc
     1261 ttgaacaggt catcatgtct gaatccggag acatcaccca ctgcactcac cagaggagct
     1321 ccagttggag ggctcaccaa atccaaaatc gccgtttctt ctaccgcttt agcggctacc
     1381 gaaatgtcca gcttacctcc cttgtcccaa ttggtcagat ttttagagcc ataaatcgta
     1441 gctcctgtgc cgtacagcat atgatctgaa ttaaatgaat ctatttccag atctccaatc
     1501 atccagccca gcttgggcga tacttccggc ggtgcaggat tggcattgaa agtcagccaa
     1561 ggcgcaccgg atatatccag attgtacttc agcttacgag ctggataacc atcaaattcc
     1621 caaatagggc tccaggttgc cccacgatca gtactgcggt aaatcgtttc atccggccac
     1681 caggcattca acgttgcaac cataagtgta cctggcttct gggcgtccac tgtcagtcca
     1741 ccatatccaa aatgattgtc tgcgctgctg ctggcaacgg ggctaatgtt agtccactgt
     1801 ccactggctg tattcagttt ccacagctca ccctttgttc catcatacgg tccagctcca
     1861 tcgctgtaag aaatatacag gtcgccatct ggcgataaaa cgccgtgatg tggcaaatag
     1921 cctgtaggct ggcccgctat agcagaccag gttgtcccgc catcagtact gcgatagaca
     1981 ctttgctttt tatctgcgac acccacatag atggtctgtg ttgctttact ggaagagccc
     2041 gtttttttgt caaaagtgat ccaggctaaa ccgacaatat cactggtgta ttcattggaa
     2101 gggtcctgta catacgtccc cggattcgga aaactactca ccttgctcca ggtcgccccg
     2161 taatcggagc ttttccacaa tccattacca cttcgagctc cgaagaataa tatattgttt
     2221 ttattcggat caattgtcag acgttcaccc atcgagcgtc ccggcatatt gcctcctacc
     2281 ttgaagggga gcttcgtcgt ctgccacgta ttccccttgt cggtagagcg cataatttgc
     2341 ccgttttgtt tatcccagga attagtatac gtgcctgtag ctatatatac acgattcgga
     2401 tccacagggt cggtggctag tgcatcgaca ccatttttgt tccaatcttc ccagcccaca
     2461 gaatccatca gcggaatcca gctttcactc gcaggattcc agcggtaagc tcctccaata
     2521 tctgtacggg catagatcag atctttttcc gtttcattaa aaataatacc aggtacaaaa
     2581 ccacctccac cgcctgtaac gacacttttc caagtataat cgtcacttgg agcagcatgg
     2641 gtgggtgcta tcatgcctgt ccaggaagca cccgctacta taagcgccaa acatacaatt
     2701 ctcaacctgc tttttgcctt agtcaatgaa ttcaacctcc ttctccgtaa tcatttaaaa
     2761 atgcaagtca tttcgagcta aaagtaagca gtcaattgcg gattcgttcc tatcgttatt
     2821 attccattca tcaatcaaac aagcgttctc tcaagctcac ctccagtaat ccaatagatt
     2881 tgcaacctct tttttgttag cgcttacatc ggtgtctgct caaaagattc gtcatctttt
     2941 tctcatttcc ttgtgtgaaa aatcaagata atgtaatagg atcttctgct tgataacttg
     3001 taataaaaga gttggcttcc acttacagtt gatacctctt ataaattact aatatgaatt
     3061 tttcatataa tgaattattt tctttaaatt tcattcgaac gaggtaccaa aatactttga
     3121 tttacagcaa aaaaccgcgc tatgcgcggt tcgggcttcc gtaatgctat ggctattcat
     3181 taataggttg aaattactgc aaatattcaa gcgccagcgc taaaaaaata tgtcctgaga
     3241 tgagtaacgc acgctcatca aaatcaaaac gaggatggtg gtgagcaaaa tgggttcgtt
     3301 catcctcatt acgagcgccc acaataataa aagctccagg tcgttgttct agataatagg
     3361 caaaatcctc tgctcccatg gccggtttta agtccataaa agcatctgct ccatacagcc
     3421 tactgactag ctcacgtacc cgttctgttt cggcctcggc attcaccaga gcaggataac
     3481 cattcaaata atcaatttcg tatcccgcat gataagcatc cgttagacct tttacgatac
     3541 tacgaatttc gttttcgact tccttgcgta tatccttatt aaacgtacgt acggtccctt
     3601 caattttggc tttatctgca attacattaa aggcagttcc tgcttgaaat accccaatcg
     3661 ttaacactgc tgattccgta gggtccactc tgcgactcac gacctgttgt agtccgttca
     3721 caatttgact tccaataaca atggaatcca ctgtctgatg cggtctggcg ccatgtccgc
     3781 ccttgccgtt gatttcaatt gaaaaggcat cggcagcagc cattgccggc ccacccttta
     3841 agccgatttt gcccagtgga atttccgagg aaaggtgaat accatatact gcttccaccc
     3901 catccagaca accatcctca atcatgaatt tggctccgcc aggcggtttt tcttcggcat
     3961 gttgaaaaat aaacacaagc tttcccttca gtgtcttacg gtgatggctt aacactcgtg
     4021 ccactcctag caacgcagct gtatgaccat catgtccaca agcatgcata acaccgggaa
     4081 cggtcgattt ataaggcgca tccttttcat cctgaatagg aagtgcatca aaatcagcac
     4141 gaaatgcaat cgttctacct ggctgcccgc cttctaaaaa accgagcaca ccgtttccac
     4201 cgacattcgt tcttacttca ataccaaaag agcgtaattg gtctgcgata aatgcagatg
     4261 tattcgtttc ctcaaaagaa agctctggat gctgatgcaa atatcttctc cacgcaatca
     4321 gttcttgttc accatctttt agttcctgaa cccatttctc cagcatatta ccctcttttc
     4381 ctattgaatt tatatacttc tatccttatc ttaccataaa tgattccaat aagttatata
     4441 tgaattacca aataactgta tacctgcgtt tttaccaatg tcaatgcaag ttctaattgg
     4501 aacgatccat gaacagaagg cttgaaatga caacaaaaaa gccgacaaaa tattctcctc
     4561 ggcttttttc tggtggtttt ctattatgaa tttttcatgt attgaaaaac tgacttatat
     4621 tccctcgtac tggatgtgtt tgctccctat actcaccatt cgtatagctt ccaatgacct
     4681 tgcgccaaaa ggattgggct ggtctattcg tgctcatctg agtcaccttc catcgcccat
     4741 taaaacgtcc aaacagctca tgagccgccc aagtgcccac gccattgtgt cgataaggac
     4801 gaagtacaaa gaactcagcc atgtaatagt cagcttctcg attgctagac attcggtcaa
     4861 cgagtgcaaa gcccacagga atatcctgtc tccagatcaa aaaaggcagc ttttgttcgc
     4921 cgctcgtcca gtattgatac aagtctggat aagccgggaa tgtccccttc tcagtgacat
     4981 caattcgcag ataggctgta aaatcgtata agtaaaattg catgagctgg acaataatgt
     5041 ccttctgcga ctctggtaca ctctttatgg acagcttcaa ttctgatttg atattatatc
     5101 ccatactttc acctctcttg gcggtcagct tgttctttac tttgattact cccatccagt
     5161 gctgttcaaa atatgctaaa ataaatgtat tatgaccgga cgaggtgaac cctatgaaca
     5221 tacaaaagat catcgaccgg cgcaaactgg atgaaaagtt gccggatttg ccatggtacg
     5281 tacagcaatt tatggattac aagcttcccg acctatcccc ctctactttg ctggaatatg
     5341 tgcgggatta cgaagctttt ttttcatggc ttcgctcgga aggcttgtct gccggagaaa
     5401 cgaatgccca agtgacactg gaagaattag agacactcca tatggatagc attacaggtt
     5461 ttcgtttgtt cctgtctacc aaacgcgaag gctccaattc ccgcatcacc atttcacgta
     5521 agctatcgtc cttgcgttca ctctttcatt atttgagcca aatcgctgag gatgaaaact
     5581 tttatccgtt gctcaagcgc aatattatgg ccaaagtcga aattaagcgc gtacataaac
     5641 ccaaggatac ggccgccaag ctcaaaggaa aaatactgga agaggaagaa ctgcttgaat
     5701 ttatcggcta tatttatgaa ggctatggac aagatttggc tgataacaag caagccctat
     5761 actcctatga attgaacaag gagcgagatg cctgtatcgc aagtcttatt ctgaactcgg
     5821 gactgcgtgt atccgaaatc gtcaacttga acatggacga tctcgatata aacaataaat
     5881 tgctgtatgt gtaccgtaaa ggaaataacg atgaaacttt taaaacgcca gtatatttcc
     5941 gcgagcaatc caaagatgat ctcgcacttt atttatcctt aagacatacc cgttatcgcg
     6001 ctccaaaaaa agaaaaagct ctttttgtcg cgcgacccaa cggcagtgct gagggcaaac
     6061 ggatgactaa aagagcgatt caagcaatga tcatcaaata cgccaagcgg ttcggcaagc
     6121 cctatttgac cgttcacaag ctccgtcatt cctttgctac agactattat ttacaaaatg
     6181 atatttacaa aaccaaggaa cagctgggtc acgcttcgac agaaacaacg gaagtctatg
     6241 cccacctgac tgacaaaacg atgtctcagg cgatcgaacg acggatagag actcagactg
     6301 actccgctaa ttagtagccc tctcatccct gctctgtctg gaatcccatt ttgtgcctct
     6361 gaatctgttc gagtagccag cagcctgccg gttcactggc atcggcagga aatatttgca
     6421 ccgtttgttc atctgagtag tagtcctgag cccaatccat gtggtactcc caaactacta
     6481 ctccctgtac atttgtcagt tcctgaacaa gctgcgcatc cgattgttcc cgcaccatga
     6541 tcagcttcgg atatttttcc tgcttgaaac cttccagcaa cacacagtca taggcctgaa
     6601 aatgccggat gagtccgatc acaggccgac tatgttcctc taaaatggca gttcttcctg
     6661 ccgaaacaat cgctacgcct tcggcacccg cctgtcggtg cttatacgta tctgtcccct
     6721 catgatccat ctcaaattca tgaccatcat gcttgatcac agctacacgc agtcctgact
     6781 gctgcgcata acgaatcaaa cctccgagga aggttgtttt tcctgtattt ttgaatccga
     6841 cgatttgaca tacaaatgga tatttcgttt tcacgtcacc tgtccgcctt gaagcaccag
     6901 tacgtgcacc tcttcacctt cgtgcaagcc ttctgttgtg ggaggaacta cgatcagaca
     6961 atcgctgtcc ttaatcgtaa ccatagcact ggattcatct actcgcgcag gaatcgcata
     7021 tacttgccca ctacgtatct ccagagaact gcgtacaaag cgggtatagt tgtttacctt
     7081 cgtataatct gctcctagct tcgccgtcca ggtttgcaaa tacggatgtg ccgaagataa
     7141 catcatttga atagttggtc gtacaaacag ctcacagcct acaaagcatg caccgggatt
     7201 gccagagagc gcaaataaca gcttaccgtc tatcactgcg gctgtggtta cactacctgg
     7261 acgcatcgta accttgttga acagcatatt tacatcttcc tgtcgtacga gatcacccat
     7321 aatatcaaaa tcacccaccg acactccacc actcgtaacc acaatatcat aggcagctat
     7381 cgcttctcgg acagaagcac gagcaaggtt caaatcatca tgaatggctt gcagaatgaa
     7441 cggctctcct cctgcctctc taatttgaga agccagcata tatgtattac tgtttcgaat
     7501 tttccccggt tgtaacggct catccactgc gagcaattct gatccagtgg aaaagacagc
     7561 cacccgtggc ttcctgtaaa ctggcacccg atgtacacca aaagtagcta gtaccgagac
     7621 atgccccgct cccacgcaag ttcccttact gagcaaaagc tgaccttcct taatctcatg
     7681 accaatttgt gtcacatttt tgccaacttc catacgcttc ttcaagccaa catatgtctg
     7741 tccattttcc tcacgcagct gtgtcgcctc cagcatcacc accgtgtccg ctccttcagg
     7801 tagttgtgct cctgtcatga tgcggactgc cattcccgtc tccacccgct tgtccgatac
     7861 ggtgccacaa ggaatttcat caatcacgtg tagccagatt tcctgaccgg tcgaacagac
     7921 ttcagtgtcc gaggcaagga tcgcatagcc gtccatacca gaacgacgaa agcggggata
     7981 cggatgtggc gcagtcaaat ccatcgccag atagcgacca tgtgcttgct cgagccctac
     8041 gtcctccctt tcaccctgtt gaacatagct agctatcctg ctttgtgctt cttccacctt
     8101 aactgcttta cgttggaatt tttctgtgtt taatggcgtc tgtttcatgt tctcactccg
     8161 ttcttctaaa ataccctctt tctgttataa cgctaaaacc gttatctggc aaatccgaac
     8221 ggtgtttatt ggatactgca agtctctatc cgccaaaaaa cagcctgaat atcatatcgc
     8281 ggctgttttt ggtagatttt gcttttttgt tttgcatgtc atcacgtcag ttttctacgt
     8341 atattaggtt atattatttt taccgaaata gattgaatct attgaaacct catacaacga
     8401 ggacatttgc tgcgctcgag ctattgttaa atcggatgaa tcttcctcta tatgttttaa
     8461 ttcattcatt gacataccaa gtatattgca agctattaac tcactataac ctgaagcgag
     8521 tcgtgccatc tttattgtat aaaagctagt gcaagttaca ttttcatttt tatctgcaac
     8581 catccaatca actccacaat ataattgata aaaaagtaat ttacctgtat actctgaacg
     8641 aaacactttt cccctgttag gagcaagtaa ggtgcaaaaa cttactcggg ggaaatgcct
     8701 tttgaaagag gagctcaaat ctcatggacg cactcaatca tggctcgccc gacgaactgg
     8761 atattcgcgg caacaaatat ccaactggat cgcgggacgt gacaaaatgt catttgaggc
     8821 ggcagtcctc atttctagga tattggagtg taaagctgag gacttatatg agtgggaact
     8881 tgcctgacaa acaggcattt ccttcggacc gtttgtacat gcggcattta cattatatat
     8941 cccgctttca ccctcctttt cttatatgta gattgttttc atcttaattt aaaagattgt
     9001 tatttttttc ataatcaaat tttatcacat agaaaatgat taaaaacggg aaatatgtcg
     9061 aattaaatcg aacccgtccg gcttatccta gtctaactga ttattgactt attcatttaa
     9121 atctggtaac aaaaatgacc cctgaatttt catgggtcat ttccacaatt ttttaagtgc
     9181 tttaagttta ttatgagaca tcgagcaagt gaagtctgat ccttcgaatc gtaccaaacc
     9241 tccaaatgcg ttagactcaa tctcacaaac caattctagg tttatcaggg tgtctttata
     9301 agcaggatat agatacggaa aagcttccct gcaagcttga gcggttagcc cgattgaata
     9361 agagcgatct ccagagtgaa aacgcgggac ataataattt tttttaggtt cccacatatc
     9421 cacactaagc atattcccta ctttaaaaaa accgctcact gccgctttac ccactacctt
     9481 aatttccgat ccgaaaatat aacgaatttc tctatcggca ttcattgcat tctcatatat
     9541 ttgtttccat cgagataaat ttgctgaatt tatactttct ttaatgtctt gatttttaaa
     9601 aacaacctgc ccccctccat aatctcccgt aacgatcttg tccacttgtg tgatgttaac
     9661 caaattagat ccgtccaaat tgcaaaaagt gggtaagcct acggaacatt catggagggt
     9721 ggtgggaact gtaaatcttc catgagaagt atgaaaaagc gggatgtgat aattgctttt
     9781 gggcttccac aattcaatcg caactaggtg attctcacaa aaaaaagagc aatctccaag
     9841 tgaattgtca tcaagcaatg gagtagctag gattcgcttt ccctcttcca ttaaattcag
     9901 ttcttttctg ttttcttcag ctcagctagt acaggtttag cgccaaaaat tgttttttct
     9961 tttttggtag actcgttagc gtaattacca aggcttatac ttaaagctct cgccaatttc
    10021 ttatccattt tcgctcacct cctttccata aaggaagaat actgaatgct tgtataatta
    10081 aagataaaca tattatgtga gattgtataa aaatatttaa tatgcaaacc aaaatacata
    10141 tcgttttgta atatttgtga tgtttggtct tgttaatata gataaaatga ttgggagcat
    10201 atactacaaa cattaataat gaacaagcgt taattattaa aatcgttgat aaacttaaag
    10261 atacgaaagg tatagttaca catatggccg ttgtaaaaca gacacaaagg gtaaggttgc
    10321 tgaagtgtac ccctcctgaa aatctcctta caaacatgat cgtagccagg gctagtaatg
    10381 ccccccaaaa atgtcctgtt atccaggcca taactgcgac cattagtact gccgaaattt
    10441 cggtaatttt cacaccaatc acatactcca tcacttctac acttgtaggt ccatcaggat
    10501 cagctttctt tatttctgat gcgatgtgcc tcgatagcgt ttctagcatc ctcgtaatct
    10561 cctttctctg ataagaagta cgaaattcca aaagtcagca atgagatgga aagtagtcct
    10621 atcgtattgg agttatagag catatacaaa gtaacaagaa tcgtgacaaa tgatattaag
    10681 gagcctataa tgaacatttt attaaggggt gataggtaat tttcaccttt caaaaaatca
    10741 tgtgggggct gaatgataaa tgagaaacca aagttgtatt tccccatgac aaaagaaata
    10801 aagtacgcag taaaaataga agctagctgt ataacgtaaa tggaagagcc agctgtatca
    10861 cttataacac ctggttgtgc tactccgaaa aagtttgcaa agatatatac aaatagctgt
    10921 agattaatgt aagctgtaag accggaacca gcagaaaaag ccgcaagata tgttttgatc
    10981 cctaaaccaa atcttaaaaa gagaaccatt aacaaatact gaagcggaaa atcaaattta
    11041 ggcatgccta taacttctct catgagaaag gaaaacaagg ctatgcccat tgcatagaaa
    11101 attatttttc ttcgattttc ccacacggga agcatataaa gcttcaagac caaagtaact
    11161 gccgctaaag catcgaaaaa accaagtata atatataaga aagcagataa cacagttaca
    11221 tcatcctcct atttcgcctg atgtaattgt atattttact aatagatgtg gcaatgaaat
    11281 aatctagtaa actggaattt tttgtatgaa tatgagatga gattttctcc ccctggtttt
    11341 ctttttcaaa ctcgacatcc attcgtggta ataaaaaaag tcaccccaag tgagcgacgt
    11401 tgcttttgcc aatgtattat gttggttttt tttcgatatc aaggttataa tctacttaat
    11461 ttggggtgga gggattattg tggctttaag tgacaatttc aaaaaattac gtataaaata
    11521 tcatttgact caacaagagc ttgctgagat cgctggggtt accaataaag caatttcagc
    11581 ttgggagagc ggactttctg aaccacggat gggtgctata gaaaagatag ctcgtcatct
    11641 ccatatcaaa aaaagcaata tcattgaaga agatggcatt gagcacataa gccctgatcg
    11701 caggtcaaaa tagacggcaa taaaatcgaa gtgagggagc atccattcaa tttacattag
    11761 taaatgttaa acaacaaaaa aaggaactgt gcaagacagt tccacagttc cgcaaacaac
    11821 tttagaagaa aataaaatat tacccatcat cgtcgacttc gccggctaat tttacctgag
    11881 ccgctgcgtg ttttaggctt actataagat ttgcgcggtg aatcaaatat gctggaacca
    11941 ccgctgctgc ctcggtcacg tgaaatcgag cctctgctcg gtgaactgct ggagcctcga
    12001 tcgaatagac ttccgcttga tccaacgctg gaatccgaat ttttccctct acgggtaact
    12061 gaaccttttc gttccaccgt tatcggcgga atcgcttttt tctcctgtgc tgttggagca
    12121 cgatattgcc cttgtgaagg tcggtataca tctctgctcg aataacctcg ataggagcca
    12181 gacccccgca atgaatcgaa cagagcatcc agcacagttg cagttaagta tccttttaga
    12241 aagctggaac tgtaattttg ctcgacatac tttttactgt cgatttcaat gagtgtatct
    12301 tccttcttag ctggatcttg ctgcaaatgg taccattcat cgctatatac cagaaacatc
    12361 cgctcattgc tctgaggcga tatttgttcc ggtgtacgct gttctttcaa ttcctccgct
    12421 acttcgggaa ccgttttgcc tgccgcccga tacacgtacg aggtcgagtt tccgctacca
    12481 ctaacggatt ccagcggata cgtatctttc acatttggcg ccccgcagcc gcttagcacg
    12541 gatacgaaca agctcagcgc cacaatgatc tttaatgcat attgccaggt gcgtttgctc
    12601 atgtgtaacc ctctcctagc ttgcccgaat aaccttgacg tctccggtaa tcacttcttc
    12661 tccttcataa agcataaagc gaccgtcctg ccattctacc cgcaatagat ggtaatcatc
    12721 agactggtac tgccaaacat gttgctctcc accctgccgg aaaggagcac ggcccataac
    12781 ggtaacatgc ccaaaatact gttcttccaa atgatacgtt gtatcatcca gttctaaaat
    12841 ggtcggtact tcctccgggt tgtctagtct gccatcgata gcctgatata gtccgaattg
    12901 cagtgtttcc cgttcctcaa tatgaagata actcacttga ttaccatcct gaagcgtcaa
    12961 aacaacggca ttccgggcac gattatgtac acgacctacc acttcatagg tgacaagcga
    13021 cacttcacaa atatccccag gatttaagtg aagcatgctt ttttccgctt tcggcggttc
    13081 aggcttggca aatagatttg aaattcgttt ccatacactc atggtcatat ttcctcctta
    13141 ctttgcctag aagcatgctg taatgatcaa ggcacccacc aaatgcagcg ctcctgaaaa
    13201 cagaccgaaa ccaattttac cttcttgggt acctttgtcc agatcaaact gtgcccagta
    13261 ccgcaacagc agacgcacaa tcacttccaa aacaagcaag atgataaagg caatcacaga
    13321 atacacaagc gcttcgagca aattattagc tgtagcaatt gaacccgata aaatgtatcc
    13381 ttgtgcaatc agcttcataa tcatccggac tgttacagcc atatttccgg cttttacttc
    13441 ggcaaaatcc ttatactttg taaataaaga atcgacatac attaatacga acagcaaaat
    13501 agcacctgcg cccgtccata ccagcatcgc tttcatcgtt tctaaaaaat gcatttgcta
    13561 ccatcctcca catcttcttg actacatata ttgatttacg cccccaggca gggaaaagcg
    13621 aaggagataa cgtttctcct tcgcttactg agccaccaca ctagcggcaa tgcttaaaag
    13681 ttcttatttg ttttcgtatt gtttcagcaa agcagcaagt tcgtcgtcca cagcactttt
    13741 cttatccagc tctttgaact cttcatccag cgatttttcc ttggaagcca tttcgttgct
    13801 cgcttcagca cgtgcttctg cttgcagcat tttttcctcc atacgcttta atccagcagt
    13861 tgccgtatcc gagctaaaac cattcatcgc tttgttaatt tcagtttgcg ctttagctgc
    13921 attataacgg gcaaccagtg tctcgcgctt gtttttcatt tcagtcagct gtctgcgcat
    13981 ttcatccagc ttaccacgca ggttgtcagc tgcagctttg ttttgctcat aaccagcttg
    14041 gaactcgttc attttttgtt cggcagattt cttttcttcc agcgcacgac gagcaagatc
    14101 cacattttgt gcttttgcag ccgtatgagc ctgttcttcg cgttttttca ccaaagctgc
    14161 ctgttcttcg tattgctgtt taaatctctt ctcaatagca atttgagcgg ctaccgcttt
    14221 ctctgcgtct tccagatcct cttgcatatc acgaatgtat tgatccgtca ttttaactgg
    14281 atcttctgcc ttgtcgataa tggcgttaat gttagacatc gtaagatctc ttaatcgttt
    14341 gaaaatagac atgtgaagtt cctcctagaa ttgaatgctt aaattttaag ctctgtacac
    14401 atgtttacgc aagctttgca aagaggtttc aaaaatagct aaaaaccgcg tagataaggc
    14461 tctggaatat ccgcctgtac ttttagcgcc cgtgatgcat gcagcgccca atagggatca
    14521 cgcagcatgg cacgccctat ggctaccaga tcagcttcat tgtgctgaac gactcggcgg
    14581 gcatcctcat aatcatccaa taagccgaca gcaatgacag gcacattcag tttctcacgg
    14641 atggtatgag ccaaatccac ctgataacca gcacgcgcag acggacctcc gctggaacca
    14701 atcggaccct caccaccaga agaaatatgg aaaatgtcta cacccgcatc acgatatcgt
    14761 tcccctatgg ctgctatgta ttcaacatca tatccgtctt ggacatattc tttggcagat
    14821 atccgcatta gcacaggcat atcagcagga agtacctctt taacagcacg tatcacctca
    14881 acaccaaaca gtgataaatc ctgcccatac tcatcttctc gagtattggt taaaggagaa
    14941 tgaaattgat ggattagata gccgtgagcg ccatgaatct ctaccgtgtc aaaaccagct
    15001 tctaccgccc ggcgcgcccc ctctttgtag gcaagaacca gttccttgac ttctgctcgc
    15061 gtcaaagcgt gcggctcttt aaatcgttca ctaaagggga tcgaggacgg gcctacaggt
    15121 ggctcagcat cttcagcctt gcgtccggca tggcctaatt gaatggcaat tttggcacca
    15181 tacttgtgga cttcctctac cattttacga tatgcaggaa tatgttcatc actccaaatg
    15241 cccaaatcac gatccgtaat gcgcccatcc ggatgaaccg ctgtcatttc cacaattatg
    15301 agtccagtcc ctcctaccgc gcgactcaca taatggacat aatgccaatc cgtcggtttg
    15361 ccgtccttgg cctccacgct gtattggcac attgggggca tgacaatccg atttcttaaa
    15421 gaaagcccct ttaattgata aggagtaaac aagtcggtca cttttatcca tccttccctc
    15481 aattctaagt ctgtagcatt atgaatagca tccccattat acaccagctg tcacggtata
    15541 ccgaatcctt tgtaaggcta aaacttacga aaatgctcca gttttcgttc atgtttaaga
    15601 gaatatgcgc gtttacttcg tcatcttcac ggatatccgc ttcatgatgt gcttctcttc
    15661 agtcgtgtag gaattcactc aatcaccagc ataattttaa taggacaggt acataatagc
    15721 tgagagcacc actaggtacc actgcacgga aagggggaat acaaatgcgg tacaggggag
    15781 tgttgccatg tttagcactg ttactagcat tttcaagttt tcccgttcac gctgacacgc
    15841 ctcccaacat cggaacgccc catcccaagg aagctactgt cgctacttcg gtatatcaaa
    15901 gtaaagatcg agctactcca acattagcga tgctacgaaa aaagcatgcg gacattttta
    15961 aaatgtgcgg cccgcacacc cgccaaattg ccttaacctt tgatgatgtg cccgattcaa
    16021 ggtatacgcc gcaggttttg gacgtattac acgcaaacgg tatcaaagct acgtttttta
    16081 tcgtcggcca tcgtgcggaa aagcatccgg ggatcatacg ccgcatcatc cgcgagggtc
    16141 atgctatagg aaaccattcc tatactcatc cggatttcag gaaggccaca ccagaacgct
    16201 ttcgccacca gattacaaaa acagaacgta ttttagaagc cagcgcaggt tttcggccca
    16261 gattgatacg accaccctat ggtgaaatca cggagcctca aattcagtgg gcgcgtcgtc
    16321 atgggtacat gatcgtcaat tggaatgtgg actctctaga ctggaaggga ttaagcaaaa
    16381 cacagattca gcgtaacgtg atcccggcta ccagacctgg ctccattatt ttaatgcatg
    16441 caggtggagg tcaaagttcc aacttgcaag gtactgtcca agctttgccg ggtctaatcc
    16501 actctttaaa agctaagggc tacaagtttg tcacagtccc ccggctactt cacacatcgg
    16561 aacgaaaata acagtatgtc taagcctcgt atataccaac aacgtaaaaa ggctgccttg
    16621 acgagcagaa tgcttctgct tgtaagacag ccttttttag cgataaacga ccgtactgat
    16681 aagaagtcgt cccagttata tgttgtgtgc tataatatta agtttacata atatatatta
    16741 tgtttaatga attcaaatat atgcacaatg ctatttatca ataacgtaca gtttcacatt
    16801 ttggaagccg aagtcgttca cgaatgattt gttgccaggg ataaagatat caatccgctt
    16861 tccggtaata gcgctgccct gatcacgggc aacggctaca aaagaatggt ctggcaagcc
    16921 gggatgggaa tagcctgtaa ctagcacctt tgttcccata ggaatgactt tcgggtctac
    16981 tgcaattgta cccagtttaa gcggattacc gtagtaatcg actgcacccc atttgccatt
    17041 ttcatcagct gcggctgaat atgctgttgc tttaacatca acaactttac tgtaattgaa
    17101 tgttttgccc catgcttcaa ttgttggcct ctcattcgct ggtgctttct ccgtaagaag
    17161 ctgtggtttc gaatccgaag ccgccttcac acttttttcg tctggcactt taatttgtag
    17221 acctgcatat atgttgttgg acgcaatatc cgggttagcc ttcgtcaagt catctaccga
    17281 taccccataa cgttgagcaa gcaaaaagaa agtatcgcct tctttggcag tatgaagcga
    17341 agcagcattt gctactccag ctgtctgtgc caatcctgca atcgctgcgg caagcaccgc
    17401 aaatttcatg gtttttccaa tatgttttaa gtgtttcaaa gatatgtacc tcctctgtta
    17461 tgcctgcgaa gttagctgtc ggattcggac tcaggagaag ccctatgctt gtgcaattca
    17521 cccctaggca tattccacaa catctatatg acgcattgga ataattgccg ttgttacgtc
    17581 ccccgcactt cccatggttt gagcccagca cctgagtaat tgttatcatt gctgctgccc
    17641 cggtaagttt tggcttactg cataatatca caattttgta accttatctg catgaaaatg
    17701 ttgggaacct tgaacatcat actatatcca ttacaatttt gtcatcaaaa atcggttttt
    17761 tgttacgaaa atatggagat atggtgacaa aaagagaaaa gccctccgtg aaatatcatc
    17821 acgagagggc tttgtcaata attcataaaa gcattttctt acagcacttt agccagaaaa
    17881 tcacgggtac gatcatggct tggagttccg aaaacttgct ccggcgtacc ttgctctata
    17941 atttgaccgt tgtccataaa gatgattcgg tctcctacct cacgagcaaa gcccatttca
    18001 tgcgtcacga tcaccatcgt catgccgcct tcagcaagct tcttcatcac atccagaacc
    18061 tctccaacca tttcagggtc cagggcagag gtaggttcat caaacagcat tacgtgcggc
    18121 tgcatggcga gagcacgtgc tatcgctata cgttgctttt gaccgccaga taactggttc
    18181 gggtaggtat tcttcttatc ctcaagaccc acggtccgca ggagctccaa cgcaattttc
    18241 tccgcttctt tccccgattg cttcttcacc tgcgttggtg caagcgtcag attctgcagt
    18301 attgttttat gcggaaacag attaaaatgt tggaaaacca tccccatctt ctctcgagtc
    18361 gcattaatat tatgaccacg ggccgtaatc ggctcaccct caaacaagat ttccccttct
    18421 gttggctgtt ccaacagatt catacaacgc aaaaacgtac ttttccccga accactcggg
    18481 ccgataatga cgactacttc acctttggcg atatctatat caatgccttt taaaatttca
    18541 ttcttaccga atgatttacg cagttgttta acggtgatca ctggctctca acttcctttc
    18601 ccataaaccc aacagcttag ataagctaaa tgtcattaca aaataaatag cggctgcgat
    18661 tacgaacgga cttaagcctt cgtaggtaat attcttaatg acctgggctt gatacataat
    18721 gtccaccatc ccaatggtcg aaataataga agattcttta atgatcgtaa tgaactcatt
    18781 accaatagca ggcaatatag atttaaatgc ttgcggtaaa ataacgtgac gcatcgcttt
    18841 gcctcgtccc atcccgagcg agcgggcagc ttccatctgt ccacggtcta cggcctgaat
    18901 accagcacgg aaaatttcag ccaaatatgc cgaactattc agagacaacg taattatacc
    18961 ggactggagt ggcgtaaaat taacacctaa cgtcaaagca actccgtaat gaatgatcat
    19021 aagctgcacc agcataggtg tgccacgcag tatctctaca tatgcggtac ctagccactc
    19081 gataatacga ataccactca tccgtagcaa tgatatgatc aggccaataa tgaatccgaa
    19141 cagtactccg agcgcagaca gcaatagagt gtagccgata cccgtagcgt agaaactgcg
    19201 atatttcaaa aatatatcta caatgctatt cgaagaaacc tgtctgtcag caaacagtgc
    19261 actcgcttct gtagaaaact tctcaatcga accgtccgct ttcaggcgag ccaacgtttt
    19321 atttactgaa gcaagcaatt ccgcatttcc tttacggatg cctacagcag tttctgctcc
    19381 ttcatcttca ggtactgctt tggcaagtgt tacggtaccg tctagcgtgt atcctttagc
    19441 aacggtatct tcgattacaa ccgcgttgat acgcttggtc cgaagctgga gtaccagatc
    19501 ggatatttta tccaatgagg ttaccgaagc attaggtact ttttgcgcaa tttcttcctg
    19561 gatactcccc ttctgcgcac cgagcttctc tccttttaga gcagccatcg ttgaatattt
    19621 gtctttgtcc tctggacgaa ccataatgac ttgcttggaa cgatagtata catccgaaaa
    19681 atcaatgctc tttctgcgtt catctgtcgg tgtcatacca gacagcacca agtctacgcg
    19741 accactttgc agagcaggca gcaaagagtc aaaactcatg tcctctggga ccatctctgc
    19801 atccagatcc ttggcgattt ccttggcaat ttcaacatca aacccgacaa tggtatcctg
    19861 accgttaata actttgtgaa actcatacgg cgggaagtca gcactcatac ccactacaag
    19921 ttttttctgt ggctttcctt ccgatccgta tgcagtgagc ataggaagac cagccaacag
    19981 caagataacc gccgtcaaaa ccagggcggt aaaacgatat gcctttttca atcttttttt
    20041 ctctccttaa attctttata ataaataaga ttgtgtcatt ataaatgaat atgcatgatt
    20101 atgcaatgaa tgttatagta caattttagt atatctatcg cagtacctac attacagcat
    20161 atataaagga gttttcagca tgaattttaa acagaccatt caacacacca ttgaacagca
    20221 tgccgagcga tttaaggaca tttcgttgta tataggcgca aaccctgaac ttggaaacga
    20281 ggaattcctg gcctctgccc gtctaaaaga agaacttgcc tttcacggtt ttgaagttca
    20341 ggcacccata ttgggtatag aaacggcctt tattgctact tacaaagcag tcaagcctgg
    20401 accagttgtt gcttttctgg cagaatatga cgccctgaaa ggtttagggc acgcctgtgg
    20461 acaccatttg atatgcatga tgagtacagg cgctgccgtc ggtctcaaat cgatcatcga
    20521 caagactggt ggagaagtac gtgtttacg
//
