LOCUS       NZ_CP061870            20171 bp    DNA     circular CON 25-MAY-2024
DEFINITION  Bacillus subtilis strain FX-1 chromosome, complete genome.
ACCESSION   NZ_CP061870
VERSION     NZ_CP061870.1
KEYWORDS    RefSeq.
SOURCE      Bacillus subtilis
  ORGANISM  Bacillus subtilis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 4266037)
  AUTHORS   Huang,S.
  TITLE     Identification and cellulase producing ability analysis of Bacillus
            subtilis FX-1 based on Genome-wide data
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4266037)
  AUTHORS   Huang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2020) Technology Innovation Center for
            Exploitation of Marine Biological Resources, Third Institute of
            Oceanography, Ministry of Natural Resources, 184 Daxue Road, Xiamen,
            Fujian 361005, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: FALCON v. 0.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 490.0x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022647025.1-RS_2024_05_25
            Annotation Date                   :: 05/25/2024 05:42:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,620
            Genes (coding)                    :: 4,349
            CDSs (with protein)               :: 4,349
            Genes (RNA)                       :: 123
            rRNAs                             :: 11, 10, 10 (5S, 16S, 23S)
            complete rRNAs                    :: 11, 10, 10 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 166 of 271
            Pseudo Genes (incomplete)         :: 103 of 271
            Pseudo Genes (internal stop)      :: 45 of 271
            Pseudo Genes (multiple problems)  :: 38 of 271
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 16:12:52
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2077898
            Orig. end                         :: 2098069
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP061870.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..20171
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="glycocin"
                     /region_number="3"
                     /rules="(glycocin or sublancin)"
                     /tool="antismash"
     cand_cluster    1..20171
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(glycocin or sublancin)"
                     /kind="single"
                     /product="glycocin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20171
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:10171](+)"
                     /cutoff="20000"
                     /detection_rule="(glycocin or sublancin)"
                     /neighbourhood="10000"
                     /product="glycocin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..10171
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(glycocin or sublancin)"
                     /neighbourhood="10000"
                     /product="glycocin"
                     /protocluster_number="1"
     misc_feature    500..515
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 21.41"
                     /tool="antismash"
     misc_feature    complement(501..514)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: strong, score: 22.43"
                     /tool="antismash"
     gene            666..917
                     /locus_tag="IE382_RS10855"
                     /old_locus_tag="IE382_10805"
     CDS             666..917
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389631.1"
                     /locus_tag="IE382_RS10855"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IE382_10805"
                     /product="PH domain-containing protein"
                     /protein_id="WP_041336407.1"
                     /transl_table=11
                     /translation="MLGINVEKSNENLTINWQLSKIEIPIKEINDVFLDPNYGGEEKSA
                     VRIGFPYGSTDRVVIKTANNTYILFTTNAASIMSKIVS"
     gene            1008..1541
                     /locus_tag="IE382_RS10860"
                     /old_locus_tag="IE382_10810"
     CDS             1008..1541
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017696906.1"
                     /locus_tag="IE382_RS10860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IE382_10810"
                     /product="SMI1/KNR4 family protein"
                     /protein_id="WP_041336408.1"
                     /transl_table=11
                     /translation="MEEGKLIHLTLAGIKEAVSKYGTFPMIQPGGFVLEDATFEFNEPA
                     TEDEIRKLESHFNVSLPKDYKEFLALHNGLELLDGIEILSIEDVMKYNETEDLPEKCFL
                     IGYHFDGRYVIDSNRYEKGNLDYLFYLDSIEHFDEAVDLKANFEIWFDRLLSSNGNKYW
                     EVERDAKAYYKNIN"
     gene            1674..3476
                     /gene="yobL"
                     /gene_synonym="rttL"
                     /locus_tag="IE382_RS10865"
                     /old_locus_tag="IE382_10815"
     CDS             1674..3476
                     /codon_start=1
                     /gene="yobL"
                     /gene_synonym="rttL"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389781.1"
                     /locus_tag="IE382_RS10865"
                     /note="YobL, as found in Bacillus subtilis, is part of a
                     toxin-immunity pair, and mediates inter-strain competition.
                     The cognate immunity protein is YobK; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /old_locus_tag="IE382_10815"
                     /product="LXG family T7SS effector ribonuclease toxin YobL"
                     /protein_id="WP_041336409.1"
                     /transl_table=11
                     /translation="MKVFEADSLLSEADKRTKEYKELRSQMVKLKKAFKAVADLDDSEF
                     SGKGADNIKAFYHDHVGVTDQWIDLIDMKIAFLSSMSAKLEDAKMSDAYIEESFLEHEL
                     ANAYTKSKSIMSEQKKAMKDILNDINDILPLEIFSTEDFKDKLSSADDKREKTIDKLNK
                     LDEDLKTEYAETEPNEQFIQQDFKKLQESTGKGKNATPIHYNAKAYRESDIHKKKGDIE
                     KHSEAYLSVKKEEAKDREIKELKKKLNDGVSDPVEYLEIAKKVGYENLEPAQVQLAVQI
                     EQAKQLEGAGEITWDIVKGVGVGLYDVGKDTVTGIWDFITDPGETLSALGNAVIHPVKT
                     YDTISAAIEESYQKDMVNGDAYSRSRWVTYAVGSVAVAVFGTKGAGAINKADAAGKVIN
                     KASQVGKKIKDVKIPDLLPYNPKYDFAMAGGVPYNVVDSQSLKNELLTNAKKIPDGTRK
                     PFTGQKISPPWLNKEKYEAYEIKGKVKAKGKVKDVSRRVYTMKDIDINQKTEFGVTNLQ
                     LMKNGNAPYAKDGTQINLHHLIQEEPGPMLEIPNSLHTKYSDVIHKLKSDGESFRNDKA
                     LKAQYESFRKRYWKWRAKQFENGN"
     misc_feature    1699..1716
                     /note="TFBS match to HipB, Bacterial antitoxin HipB,
                     confidence: weak, score: 22.23"
                     /tool="antismash"
     gene            3486..3944
                     /gene="yobK"
                     /locus_tag="IE382_RS10870"
                     /old_locus_tag="IE382_10820"
     CDS             3486..3944
                     /codon_start=1
                     /gene="yobK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389780.1"
                     /locus_tag="IE382_RS10870"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IE382_10820"
                     /product="type VII secretion system immunity protein YobK"
                     /protein_id="WP_041336410.1"
                     /transl_table=11
                     /translation="MIYSKVENFINENKQNAIFTDGASHENIGRIEENLQCDLPNSYKW
                     FLEKYGAGGLFGVLVLGYNFDHASVVNRTNEYKEHYGLTDGLVVIEDVDYFAYCLDTNK
                     MKDGECPVVEWDRVIGYQDTVADSFIEFFYNKIQEAKDDWDEDEDWDD"
     gene            4034..4492
                     /locus_tag="IE382_RS10875"
                     /old_locus_tag="IE382_10825"
     CDS             4034..4492
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014479998.1"
                     /locus_tag="IE382_RS10875"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IE382_10825"
                     /product="YrhA family protein"
                     /protein_id="WP_014479998.1"
                     /transl_table=11
                     /translation="MWVNLLEEIRKTEAKYGDELNSPVTDQEIRNFEEAVLGKFPVNEI
                     PSGYKKFLQTVNGLDFNGLVIYGLDQELLREENDEEVYGFIETNEQWHENDEQKKYLFF
                     GDSDTAWYCLDVIQNEYLELDKPSGTLMNKFNDFNAMLADALKVSLDN"
     gene            4548..5102
                     /gene="yokK"
                     /locus_tag="IE382_RS10880"
                     /old_locus_tag="IE382_10830"
     CDS             4548..5102
                     /GO_process="GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="yokK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014479997.1"
                     /locus_tag="IE382_RS10880"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IE382_10830"
                     /product="SMI1/KNR4 family protein"
                     /protein_id="WP_041336412.1"
                     /transl_table=11
                     /translation="MSFNNVKQKLKEFLIDAKTDPRINKNEQPKEIEMNIGKQLPSDYK
                     DFLKEYGGCYLESKKTSDEIEYDVCYKPIEKDPWMGKGDDTQLLEGFYGLANDHDSLQK
                     AIDTYSDRFPRNIIPIASSAGGNEICMDIDNGKILFWDHELSHPDKDFFLIANSFEEFV
                     FSLVDEPIEADKEDDGILYIE"
     gene            5162..5695
                     /gene="yokL"
                     /locus_tag="IE382_RS10885"
                     /old_locus_tag="IE382_10835"
     CDS             5162..5695
                     /EC_number="2.-.-.-"
                     /codon_start=1
                     /gene="yokL"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1111: GCN5-related N-acetyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_390038.1"
                     /locus_tag="IE382_RS10885"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IE382_10835"
                     /product="GNAT family protein"
                     /protein_id="WP_014479996.1"
                     /transl_table=11
                     /translation="MAVSYWTGENLILRAIQPEDIVIFDSLDDEILRNMDSLHFPRSAN
                     NMREWVEEQLEKDEFRFIAVDSDNNIVGIIETFDCDRKNGTFGYYLAVFEPYRGKGFAK
                     EMILMVLRFFFLELAYQKVNTTVYSFNNPSIRLHEKLGFMKEGQLRKIIFTKGAYYDGF
                     CFGMTREEFELNHG"
     gene            complement(5982..6098)
                     /gene="bsrG"
                     /locus_tag="IE382_RS10890"
                     /old_locus_tag="IE382_10840"
     CDS             complement(5982..6098)
                     /codon_start=1
                     /gene="bsrG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009513970.1"
                     /locus_tag="IE382_RS10890"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IE382_10840"
                     /product="type I toxin-antitoxin system toxin BsrG"
                     /protein_id="WP_009967548.1"
                     /transl_table=11
                     /translation="MTVYESLMIMINFGGLILNTVLLIFNIMMIVTSSQKKK"
     gene            6330..6797
                     /gene="yolA"
                     /locus_tag="IE382_RS10895"
                     /old_locus_tag="IE382_10845"
     CDS             6330..6797
                     /codon_start=1
                     /gene="yolA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_390037.1"
                     /locus_tag="IE382_RS10895"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IE382_10845"
                     /product="YolA family protein"
                     /protein_id="WP_041336414.1"
                     /transl_table=11
                     /translation="MKKRITYSLLALLAVVAFAFTDSSKAKAAEALPLYYLQITGITSD
                     GNDFAWDNITSSQTKAPNVLKGNKLYVKARFMGYTKLTVITGKDGKNLLYNGTAKMFKS
                     DAILGQNKVVIGWDKYFEIPMDALQDNSIQIKALSSGTTFVYSQKIDFERE"
     gene            6803..7159
                     /locus_tag="IE382_RS10900"
                     /old_locus_tag="IE382_10850"
     CDS             6803..7159
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_390036.1"
                     /locus_tag="IE382_RS10900"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IE382_10850"
                     /product="hypothetical protein"
                     /protein_id="WP_041336416.1"
                     /transl_table=11
                     /translation="MISTSNYDVTKDPKFLAFCSLATEGYGQNNTQQSSIGFNEFCEKL
                     EKNEIVKPENKKSTLDLNKSLEDWKLRFTAGGNLSTMDSQVLDVIKNAYNLGMVNKDNM
                     LLRNEAINAYRNSI"
     gene            complement(7202..7537)
                     /locus_tag="IE382_RS10905"
                     /old_locus_tag="IE382_10855"
     CDS             complement(7202..7537)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389777.1"
                     /locus_tag="IE382_RS10905"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IE382_10855"
                     /product="hypothetical protein"
                     /protein_id="WP_041336417.1"
                     /transl_table=11
                     /translation="MKKRLIGFLVLVPALIISGITLIESNKKAPEEVLESAWDEFGLFS
                     FQIGITDPAITIGMDQTKSETKLREYLEHNLSREAKEKYKIYILKDDINKLEKEHREYL
                     KANNPNK"
     misc_feature    7632..7647
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 20.94"
                     /tool="antismash"
     misc_feature    complement(7633..7646)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: strong, score: 23.81"
                     /tool="antismash"
     misc_feature    complement(7663..7676)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: strong, score: 24.43"
                     /tool="antismash"
     gene            7710..8042
                     /gene="yolD"
                     /locus_tag="IE382_RS10910"
                     /old_locus_tag="IE382_10860"
     CDS             7710..8042
                     /codon_start=1
                     /gene="yolD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_390034.1"
                     /locus_tag="IE382_RS10910"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IE382_10860"
                     /product="YolD-like family protein"
                     /protein_id="WP_041336419.1"
                     /transl_table=11
                     /translation="MLRDRGTIKWTSMMLPEHLTQLKQDLIDVSKIEKPSLDDQQIEEM
                     DLLVSEALEFNKELRFKLFNNGFVENVTGRVHYINFEQHRLHVKDQNDNTVYINMNDII
                     GVTYND"
     gene            8035..9285
                     /gene="uvrX"
                     /locus_tag="IE382_RS10915"
                     /old_locus_tag="IE382_10865"
     CDS             8035..9285
                     /codon_start=1
                     /gene="uvrX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_390033.2"
                     /locus_tag="IE382_RS10915"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IE382_10865"
                     /product="UV damage repair protein UvrX"
                     /protein_id="WP_064627413.1"
                     /transl_table=11
                     /translation="MIDYSQFPRKNILCVDMKSFYASVSAVTRGLNPLTCYLAVVGNTE
                     RQGSVVLAASPALKKDFGIKTGSRLFEIPEDPRIHVVNPQMKLFIRVSTEITKLFYRFV
                     PEKCVHTYSIDESFLDAGKENPEEMAKAIQSSMRREFGLMCTVGIGDNMLLSKLALDLE
                     SKKTKSGIARWRYEDVPNKLWKVRPLSKMWGIGGRMERNLNRMGISTIGQLAKFPLELL
                     EKKFGIMGNQLYYHAHGIDLSEIGAPLMQGQISFGKSQILLRDYTRSEEIKAVLLEICE
                     EVARRARTHNKVGRTISLGIGYSKDELGGGFHRSKTIDLPTSITMDIYRCCLMLFNKFY
                     SGKTVRSVSVTLSNIEDDVNQQLSLFEVDNEKRRKLGFVMDGIRSKYGSKAILRAVSYT
                     PAGTALQRAGLTGGHKS"
     gene            9387..9704
                     /gene="sunI"
                     /locus_tag="IE382_RS10920"
                     /old_locus_tag="IE382_10870"
     CDS             9387..9704
                     /codon_start=1
                     /gene="sunI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_390032.1"
                     /locus_tag="IE382_RS10920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IE382_10870"
                     /product="sublancin immunity protein SunI"
                     /protein_id="WP_009967545.1"
                     /transl_table=11
                     /translation="MEYVVMIIILLALFFIFTVFLNTRYSFDEKCLVLKFGLSKTEIPI
                     NQIVSIKESDKYGVADNIDYKIGMPYAQPDRIVIETTNKRFLVFLNGAQQFIQKYKRVS
                     V"
     misc_feature    9750..9763
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     medium, score: 24.81"
                     /tool="antismash"
     gene            10001..10171
                     /gene="sunA"
                     /locus_tag="IE382_RS10925"
                     /old_locus_tag="IE382_10875"
     CDS             10001..10171
                     /codon_start=1
                     /gene="sunA"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     glycocin: sublancin"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_390031.1"
                     /locus_tag="IE382_RS10925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IE382_10875"
                     /product="bacteriocin sublancin-168"
                     /protein_id="WP_009967544.1"
                     /sec_met_domain="sublancin (E-value: 1.8e-43, bitscore:
                     144.6, seeds: 2, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEKLFKEVKLEELENQKGSGLGKAQCAALWLQCASGGTIGCGGGA
                     VACQNYRQFCR"
     gene            10229..12346
                     /gene="sunT"
                     /locus_tag="IE382_RS10930"
                     /old_locus_tag="IE382_10880"
     CDS             10229..12346
                     /codon_start=1
                     /gene="sunT"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_390030.1"
                     /locus_tag="IE382_RS10930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IE382_10880"
                     /product="sublancin transporter SunT"
                     /protein_id="WP_003246186.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 6.5e-34, bitscore:
                     114.7, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNKKKKYVHTKQFNSHDCGLACISSILKFHNLNYGIDFLLDLIGD
                     KEGYSLRDLIVIFKKMGIKTRPLELQENKTFEALKQIKLPCIALLEGEEYGHYITIYEI
                     RNNYLLVSDPDKDKITKIKKEDFESKFTNFILEIDKESIPEKEKDQKKHSYFFKDILFR
                     NKLIVFVILLTSLFVVGLAVAGSFYIKFLVDLIIPRSLRESLITITLIFISMVLIRCIF
                     DFVRSYLIIKLSYKVDKEMSNVYFNKVTKLPINFFENREDGEVISRFNDGIYIKDFFSA
                     NFVTAIIDIILILGLGVILYRTNNILFLTIILPILLLSCLAILFFDHLKKKNQKLMEDK
                     AKSTSLLINFLKNMTTVYSLNKTSFFLEKFHLTYDKQLNSTFSVAKAVISNEILKGLIQ
                     NSFTIIILWVGTRQVLNDSMSLGTLLFINTLAAFLLSSLDRILSMQSDLQQAHVASIRF
                     FDVVNYPVQQDSNENLTELDFIQNIKTVNLNIGADPMRYIVEDINLILDRKDKVLIIGE
                     SGTGKSTFAKSLSKLYKVPDKSIYLNGLDINRYDHLSIRKRIVYIDENPFLFKGTIKEN
                     LCMGEIFDQNEIENACIMSQCHEFICNLDKQYSYKLSENGSNLSTGQKQRLALARAILH
                     QPQVLILDESLSNIDPDNTKLIYETLHRMDCLIILITHNDPSNFKYNKKLVFRNNRIIE
                     SSYSENKEYSI"
     gene            12343..12756
                     /gene="bdbA"
                     /locus_tag="IE382_RS10935"
                     /old_locus_tag="IE382_10885"
     CDS             12343..12756
                     /codon_start=1
                     /gene="bdbA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_390029.1"
                     /locus_tag="IE382_RS10935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IE382_10885"
                     /product="disulfide bond formation protein BdbA"
                     /protein_id="WP_003230920.1"
                     /transl_table=11
                     /translation="MKKWIVLFLVLIAAAISIFVYVSTGSEKPFYNDINLTQYQKEVDS
                     KKPKFIYVYETSCPPCQEIKPELNEVIKKEKLKVQALNIEEKENYNTEFLDKYNLNKTP
                     TILYYKDGKEKDRLEGYRSASQIEKFFDKNGDR"
     gene            12756..14024
                     /gene="sunS"
                     /locus_tag="IE382_RS10940"
                     /old_locus_tag="IE382_10890"
     CDS             12756..14024
                     /codon_start=1
                     /gene="sunS"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_390028.1"
                     /locus_tag="IE382_RS10940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IE382_10890"
                     /product="SunS family peptide S-glycosyltransferase"
                     /protein_id="WP_004399050.1"
                     /sec_met_domain="Glycos_transf_2 (E-value: 1.6e-19,
                     bitscore: 68.4, seeds: 256, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKLSDIYLELKKGYADSLLYSDLSLLVNIMEYEKDIDVMSIQSLV
                     AGYEKSDTPTITCGIIVYNESKRIKKCLNSVKDDFNEIIVLDSYSTDDTVDIIKCDFPD
                     VEIKYEKWKNDFSYARNKIIEYATSEWIYFIDADNLYSKENKGKIAKVARVLEFFSIDC
                     VVSPYIEEYTGHLYSDTRRMFRLNGKVKFHGKVHEEPMNYNHSLPFNFIVNLKVYHNGY
                     NPSENNIKSKTRRNINLTEEMLRLEPENPKWLFFFGRELHLLDKDEEAIDYLKKSINNY
                     KKFNDQRHFIDALVLLCTLLLQRNNYVDLTLYLDILETEYPRCVDVDYFRSAILLVDMQ
                     NKLTSLSNMIDEALTDERYSAINTTKDHFKRILISLNIQLENWERVKEISGEIKNDNMK
                     KEIKQYLANSLHNIEHVLKGIEV"
     gene            14021..14467
                     /gene="bdbB"
                     /locus_tag="IE382_RS10945"
                     /old_locus_tag="IE382_10895"
     CDS             14021..14467
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /gene="bdbB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_390027.1"
                     /locus_tag="IE382_RS10945"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IE382_10895"
                     /product="disulfide bond formation protein B"
                     /protein_id="WP_009967541.1"
                     /transl_table=11
                     /translation="MNTRYVKSFFLLLFFLSFFGTMASLFYSEIMHFKPCVLCWYQRIF
                     LYPIPIILLIGLLKKDLNSIFYVVFLSSIGLIIAFYHYIIQLTQSKSVVCEIGTNSCAK
                     IEVEYLGFITLPLMSSVCFALIFGIGLKLIIKSKKLKQNQHVYN"
     gene            complement(14523..14789)
                     /gene="bhlB"
                     /locus_tag="IE382_RS10950"
                     /old_locus_tag="IE382_10900"
     CDS             complement(14523..14789)
                     /GO_function="GO:0140911 - pore-forming activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="bhlB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_390026.1"
                     /locus_tag="IE382_RS10950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IE382_10900"
                     /product="phage holin"
                     /protein_id="WP_004399099.1"
                     /transl_table=11
                     /translation="MFENIDKGTIVRTLLLAIALLNQIMVMLGKAAFIINEEDINHLYD
                     CLYTIFTIVFTTSTTTAAWFKNNYITAKGKKQKQVLKKENLFK"
     gene            complement(14800..15012)
                     /gene="bhlA"
                     /locus_tag="IE382_RS10955"
                     /old_locus_tag="IE382_10905"
     CDS             complement(14800..15012)
                     /codon_start=1
                     /gene="bhlA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_390025.1"
                     /locus_tag="IE382_RS10955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IE382_10905"
                     /product="BhlA/UviB family holin-like peptide"
                     /protein_id="WP_003246119.1"
                     /transl_table=11
                     /translation="MEMDITQYLSTQGPFAVLFCWLLFYVMKTSKERESKLYNQIDSQN
                     EVLGKFSEKYDVVIEKLDKIEQNFK"
     gene            complement(15100..16203)
                     /gene="blyA"
                     /locus_tag="IE382_RS10960"
                     /old_locus_tag="IE382_10910"
     CDS             complement(15100..16203)
                     /codon_start=1
                     /gene="blyA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_390024.1"
                     /locus_tag="IE382_RS10960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IE382_10910"
                     /product="N-acetylmuramoyl-L-alanine amidase BlyA"
                     /protein_id="WP_004399108.1"
                     /transl_table=11
                     /translation="MSVFTNSYIPVNKYTRPGLKLQGVKKCVLHYTANPGAGADNHRRY
                     FSNAQVYASAHIFVDKAEAICIIPLNEVAYHANDIQQRDSAGNPYRGVAALKPNANFLS
                     IGVEMCLEKDGSFHSDTVERTEDVFVELCNKFGLDPIDDIVRHYDITHKNCPAPWVSNS
                     QKFVDFKNRVKAKMSGKSVSKASPTKPTTSSPSSSSAVSGSLKSKVDGLRFYSKPSWED
                     KDVVGTVNKGIGFPTVVEKVKVGSAYQYKVKNSKGTTYYITASDKYVDVTGSVKTSSSA
                     PKTTSTSSSSSSIKSVGKIKIVGVSSAAIVMDKPDRNSSKNIGTVKLGSTISISGSVKG
                     KNNSNGYWEVIYKGKRGYISGQFGSTI"
     gene            complement(16223..16441)
                     /locus_tag="IE382_RS10965"
                     /old_locus_tag="IE382_10915"
     CDS             complement(16223..16441)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009513969.1"
                     /locus_tag="IE382_RS10965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IE382_10915"
                     /product="hypothetical protein"
                     /protein_id="WP_041336430.1"
                     /transl_table=11
                     /translation="MQSKIGDFTVNELEQIKNECVRLHLNYGLGIPLTKKIHNLFHEIY
                     GTSNNNEIQFNEFRNRYENGEFEALFN"
     gene            complement(<16439..17260)
                     /locus_tag="IE382_RS10970"
                     /old_locus_tag="IE382_10920"
                     /pseudo=""
     CDS             complement(<16439..17260)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_390023.1"
                     /locus_tag="IE382_RS10970"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="IE382_10920"
                     /product="hypothetical protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MARKISDYHLKKREETQKKFIDLLAQNNYIHISGDMVNSKTKVKV
                     RCRHNHTWQVNYEHFKKGTRCPECRIIEGSLKKRLNISTVKSRYALKGYEILSTYKNCH
                     SKLKAKCPEGHIWEHLPSNFFKGEECFQCKGAKKYTVECAQAAFSDRGFIPLFDTYHHN
                     KENLPFLCKEHIDLGVQYAPLHNMVRGLANCRKCYLLLFTGENSSRWKGGISSLNKTLR
                     EAVYEVWTKPSLEKYSFKCAITNSTKDLHVHHYKKNFSEIVKEALSNLSFEL"
     misc_feature    17219..17234
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: medium, score: 23.76"
                     /tool="antismash"
     gene            complement(17428..19983)
                     /locus_tag="IE382_RS10975"
                     /old_locus_tag="IE382_10925"
     CDS             complement(17428..19983)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014417854.1"
                     /locus_tag="IE382_RS10975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IE382_10925"
                     /product="peptidase G2 autoproteolytic cleavage
                     domain-containing protein"
                     /protein_id="WP_041337019.1"
                     /transl_table=11
                     /translation="MGFKYYDKNTGSYVPMSIELLKSDGVSYTAPSIKQTFEDILKQVS
                     SVSSSVTEKVDGLSNQIGNIDDFHITGTNLVEKILNALIQRRVSVTDFGAKGDGVTDDT
                     AAFNKAFEMGYAEVFVPAGTYMVKGLKVPSYTRLYGTGKLSVIKLHKDAPAYSHVITTV
                     QNSKCIIFENLLLDWNLQKTNNSISSGPNSSCLNITNSQFVWVNNVHAKDAGLHGFDVT
                     SPSYNSLTDTEDVYQPGGSKYVWINNCTATNFGDDGFTTHFSEYVFITNCYSYDGNGSA
                     HTSGASNTNGFEIDDGSMKVWLINCVSKNNCRGFEAKAHEHAPAAQNVTFLNCVSENDI
                     RSFDFRHIGFHKASDPESKTARNIMASNCTAIKPIFNDRLYAGMTPRALVISAYKNVNI
                     SNFTAIGDPSYDYKGNPAIATQYKSRNITFNNVSSSGFKTAGADIYIYGGSQKSDFVSL
                     SNINVLESALIGIRIGSAIKNASVNQASLIGYSKEGSIGLYCTNSQVDINAVNCDKYAI
                     PSKIAGKTYTSFVPKNIKGGTRIATTSGYAAKNTSLVAASSGGGQATGTASAVIATTGG
                     SKADGPRNVVIASSGGSKTTSEGSRSMVAASNNSSIEGTGSSRMVIASQGVANKTGYTV
                     ALGYAATGAPSTANTKIQLDAKNGNINLTGQVKGASTFSDYAEYFESIDGKAIPSGYFV
                     TLEGDKIRKANAGDKVLGVISETAGVVLGEAAFNWQGRYLKNEFGGLIYEDIDVTVTNE
                     DGTQRIETKTVPKENPYYEPAEDYIARSDRPEWNVVGMFGQIFVRIDGTVAAGDRIIPK
                     AGKGSKSEDGSGYYVMRITTPYSQERGYGVALCLITPTI"
ORIGIN
        1 ttagcaatgt ttcctgaaca gatttgccat ccacataaac ataagctaat agtcttccgt
       61 atttatctct gcggtcacct ttatcaaatt caagctgcag tttaccactg ttgaccaatt
      121 ctttgttccg tttagatgca tcctcaccgt atggttgaac acaagaattt ggtttcttag
      181 tttcaggtgt atcaatgagc aagtagcgga ctgtgtctac atttccattg tagcttactt
      241 taattgtgtc accatcaaca gctctgtcta atgtcacatc gaccaattcc tttttgtctt
      301 tctctttaga tttgttagta gttttttctt tagtagcttc cttttgttct gttttctttt
      361 ctacatctgt agacgcttgt ggtgtttctt tttctgtgct tactttttca gaatcatttg
      421 aactgcaggc tgccaaagat aagctcaaag tgaatgccgc aaaacctaat aatactttct
      481 tcaacttcat ttcccctttt agaatgtttg ttctctattt atatcggtaa accatcaata
      541 attataatcc tgtatccaat tattgtgaat ttaatttttc cttaatatca tcgcatttct
      601 ctttaaaact tccaggcaca tgttaaaatt tcactgaatt catagtcaaa aggggaatgt
      661 tttttatgct tggaatcaac gttgaaaaat caaatgaaaa cttaactatc aattggcagc
      721 tatccaaaat tgaaattcca atcaaagaaa tcaatgatgt atttcttgat ccaaactatg
      781 ggggagagga aaaaagtgca gtaaggattg gatttcctta tggctctacg gatagggtag
      841 tgattaaaac agcaaataac acttacattt tgttcaccac taatgctgct tctattatga
      901 gtaaaattgt ttcctaatac caataaatgt cctattagta atgtaatgct ttccttatac
      961 tgtatgacat ggcgaaatct agattattgt tgataggggg atgggatatg gaagaaggca
     1021 aacttattca tttgacactt gctggtatta aagaagctgt ttctaagtat ggcacttttc
     1081 caatgattca accaggtggt ttcgtattag aagatgccac atttgaattt aatgaacctg
     1141 caacagaaga cgaaattaga aaattagaat cacatttcaa tgtaagttta cccaaagatt
     1201 ataaggagtt cctagctcta cataatggat tggagttact tgatggaatc gaaattctca
     1261 gtattgaaga tgttatgaaa tataatgaaa cagaagacct accagaaaag tgttttttga
     1321 ttggatatca ttttgatgga aggtatgtta tagattcaaa tagatatgaa aaagggaact
     1381 tagattacct attttatttg gattcaattg aacattttga tgaagcggtg gatttaaaag
     1441 ccaattttga aatttggttt gatcgacttt taagttcgaa cggaaataag tactgggaag
     1501 ttgaaagaga tgctaaagct tactataaga acataaatta aagatgcatc caaagatttt
     1561 ttaggtttca catgttaatc attaaaggca gtcatatttg gctgtctttt tctgttattg
     1621 aaatagttga ttgtggtaaa atttacatag agtaagaatg gaaggagaat gatatgaagg
     1681 tttttgaagc tgattcacta ctctctgaag cggataaaag aaccaaagag tataaggaac
     1741 taaggtcgca aatggtgaag ctcaaaaaag cgtttaaagc tgtagctgac ctagacgaca
     1801 gcgagttttc aggtaagggt gcagacaaca ttaaagcatt ttatcatgat catgtcggtg
     1861 ttactgatca atggattgat ttaattgaca tgaagattgc tttcttaagc agcatgtctg
     1921 caaaacttga agatgctaag atgtctgatg cttacataga agaatccttt ttagaacacg
     1981 agttggctaa cgcctataca aaatcaaaat ccattatgtc tgaacagaaa aaagcaatga
     2041 aagacatatt aaacgacatc aatgatattc ttccccttga aatattttca acagaagact
     2101 tcaaagacaa actttcttct gcagatgaca aacgtgaaaa aacaatcgat aagctaaaca
     2161 agcttgatga ggatttaaaa acagaatacg ctgaaacaga gcctaacgaa cagttcatcc
     2221 agcaagattt taaaaagctg caggaatcaa caggcaaagg gaagaacgcc actccgattc
     2281 actataacgc caaagcgtat agagaaagtg acatacataa gaaaaaaggc gacatagaga
     2341 agcattctga agcttactta agcgtgaaga aagaagaagc aaaggaccgt gaaatcaagg
     2401 aattaaagaa aaaacttaat gacggtgtct ctgaccctgt tgaatattta gagattgcta
     2461 agaaagtagg gtacgagaat ctggagccag ctcaagtgca gcttgcagtg caaattgaac
     2521 aagcgaagca gctagaagga gcaggagaaa tcacctggga tatcgtaaaa ggtgtaggag
     2581 taggcttata tgatgttggt aaagacactg taaccggcat atgggatttc atcaccgatc
     2641 caggagaaac actatcagca ctgggaaatg cagttattca tccagtgaaa acttacgata
     2701 ccatttcagc agcaatagaa gagtcatatc aaaaggatat ggtgaatgga gatgcctatt
     2761 ctagatcaag atgggtaacg tatgctgttg gttcagtggc tgtagctgtt tttggaacca
     2821 aaggtgcagg agccattaac aaagcagatg cagcgggcaa ggtcataaac aaagcaagtc
     2881 aggtagggaa gaaaataaaa gacgtcaaga ttccagattt gctgccttat aatcctaagt
     2941 acgactttgc tatggcaggg ggtgtgccat acaatgtggt tgatagccaa agccttaaga
     3001 atgaactttt gactaatgcg aagaaaatac cagatggaac aagaaaaccg tttactggcc
     3061 aaaagataag tcccccatgg ctaaataaag aaaagtatga agcgtatgaa ataaagggga
     3121 aggtaaaggc gaaggggaaa gttaaagatg taagtcgaag agtgtatact atgaaagata
     3181 tagatattaa tcaaaaaaca gaattcggcg ttacaaatct tcagctaatg aaaaacggga
     3241 atgcccctta tgcaaaagat ggcacacaga ttaatctaca tcacttgatt caagaagaac
     3301 ctggcccaat gcttgaaatc ccgaacagtt tacatacaaa atatagtgat gtcattcata
     3361 agcttaagtc tgatggtgaa agttttagaa atgataaggc tttaaaagca caatacgaga
     3421 gcttcagaaa gagatattgg aagtggagag cgaaacaatt tgagaatgga aattgaggtg
     3481 tttaaatgat ttattcgaag gttgaaaact ttattaatga aaataaacaa aatgctatat
     3541 ttacagatgg cgcaagtcat gaaaacattg gtcgcattga agaaaacctc caatgcgatc
     3601 tccccaacag ttataaatgg tttttagaaa aatacggtgc tggtggttta ttcggcgttt
     3661 tagttttagg ctacaacttt gatcatgcat cagttgtaaa tagaactaat gaatataaag
     3721 aacactatgg ccttactgat ggacttgtgg ttattgaaga tgttgattac tttgcttact
     3781 gtctagatac aaataaaatg aaagacggag aatgccctgt agttgaatgg gatagggtaa
     3841 ttggttatca agatactgtt gcagacagct ttattgaatt tttttataat aagattcagg
     3901 aagcgaaaga tgactgggat gaggatgaag actgggacga ttaagcaaaa gtattgcaat
     3961 tgatgattaa gctcttgcta agagactttt cttcgattac gagttggcgt agtaataaat
     4021 taggggcagt attatgtggg taaatttatt agaagaaatt cgaaaaactg aagcaaagta
     4081 tggagatgaa ttaaattctc ctgttacaga ccaagaaata agaaattttg aagaagcggt
     4141 attaggtaag ttccctgtaa atgaaattcc ttcaggatat aaaaaattcc ttcaaactgt
     4201 aaacggatta gattttaatg gcttggtaat ctatggattg gatcaagaat tactgagaga
     4261 agaaaatgat gaagaagttt atggttttat tgaaacaaat gaacagtggc acgaaaatga
     4321 tgagcagaaa aaatatctgt tttttggtga ctcagataca gcttggtatt gtttagatgt
     4381 aattcagaat gaatacttag agcttgataa accctctgga acactgatga acaaatttaa
     4441 tgattttaac gccatgctag ctgatgcatt gaaagttagt ttagacaatt aaacaaaagt
     4501 attgcaattg ttcattgcta aagatgtgca atgcggaggg gattaatatg agcttcaata
     4561 atgtcaaaca aaagttgaaa gagtttttaa ttgatgcaaa aacagatccc agaatcaata
     4621 aaaatgaaca acccaaagaa attgaaatga acataggaaa acaattacct tctgattata
     4681 aggacttttt gaaggaatat ggcggctgtt atttagaaag taaaaaaaca tcagatgaga
     4741 ttgagtatga cgtttgctat aaacctatag agaaagatcc ttggatgggc aagggcgatg
     4801 atacacaatt gttagaaggt ttttatggtt tagcgaacga tcatgacagt cttcagaaag
     4861 caatcgatac atattctgat cgctttccta gaaatattat tcctatcgca agctctgcag
     4921 gcgggaatga aatatgcatg gatatcgata atggaaagat attgttttgg gatcatgaat
     4981 taagtcatcc cgacaaagac tttttcttaa ttgccaattc gtttgaggag tttgtcttca
     5041 gtttagttga tgaaccaatt gaagctgata aagaagatga tggaattctg tatattgaat
     5101 aagatgatga cttactaagc agttaagaaa gcgtatggaa ataactggag ggaaagttaa
     5161 aatggcagtt agttattgga caggggagaa tcttatactg cgagcaattc aacctgaaga
     5221 tatcgttata tttgattcgt tagatgatga aattctgcga aatatggact cgctacattt
     5281 tcctcgttca gctaataaca tgagagaatg ggtagaagaa caattagaaa aagatgaatt
     5341 ccgttttatt gcagttgata gtgataataa tatagtaggt ataattgaaa cctttgactg
     5401 tgataggaaa aatggtacct tcggttatta tttggctgta ttcgagccat ataggggaaa
     5461 aggatttgcc aaagaaatga tcttgatggt tcttcgcttt ttctttttgg aacttgctta
     5521 tcaaaaagta aatacaactg tttattcttt taataatcca tccatacgat tacatgaaaa
     5581 attaggtttc atgaaagagg gacaactaag aaagatcatt ttcactaaag gtgcttatta
     5641 tgatggtttt tgctttggaa tgacaagaga agaatttgaa ctgaatcatg gataatatca
     5701 cttgtgcatc gataaagtac tggattccaa taaaataatg gttttatcca taaaaacaat
     5761 tttgcaaatt atttcaatgg ttttacttaa tgtgttataa tcagaactgt aagacatttg
     5821 attggctata gcgtatttct ttaatgagaa atgcataaaa taaaaagacc agggtgttgg
     5881 cgcaccccgg ctctgtacaa aaagctgccc gtcaaagggc ttgctcgatg ttcaatctgc
     5941 gtagacctaa ccctttaagg ttcctaagct caagggaagg tctatttttt cttttggctt
     6001 gacgttacaa tcatcattat attgaagatc aacaagacgg tatttaatat caatccgcca
     6061 aaattgatca ttatcattaa tgattcgtaa acagtcattt tcccaccccc tttcataggg
     6121 gatgagcaag actcccttga gtgagcaaac tttatgtaca ggaaatatta tactatacat
     6181 aaatgtcctt gtcgttaaaa attcacaata aattgacaat atgaaaagaa cttgcttaat
     6241 gcaggttctt tttttatttc aaatattaaa caattccctt caaatgacga tatttaacat
     6301 gtaaacttgt accaaggagg atttaggaaa tgaagaagag aattacatat tcactgcttg
     6361 ctcttctagc agttgttgct ttcgctttca ctgattcatc aaaagcaaaa gcggcagaag
     6421 cattaccact ctactatcta caaattacag ggattacatc agacggaaac gattttgctt
     6481 gggataatat cacttctagt caaaccaaag cgccaaacgt tttgaagggg aataaactat
     6541 atgtgaaagc tcgttttatg ggttatacca agttaacagt tatcacaggc aaagacggta
     6601 agaatctgtt gtataacggc acagctaaaa tgtttaaatc tgatgctatc cttggtcaaa
     6661 ataaggtggt aataggctgg gataaatatt ttgagattcc catggacgca cttcaagata
     6721 actctataca gattaaagca ttgagctcag gcaccacttt tgtttacagt caaaaaattg
     6781 attttgaaag agagtgaccc aggtgattag cacttctaat tatgatgtaa ctaaagatcc
     6841 aaaattctta gctttttgta gcttggcaac agaaggatat ggtcagaata atactcagca
     6901 atcttccata ggttttaacg agttttgcga aaaacttgag aaaaatgaga ttgttaaacc
     6961 cgagaataaa aaaagtacgc ttgatttgaa taagtcttta gaagactgga aattgagatt
     7021 tactgcaggc gggaaccttt ctacaatgga ttcgcaggta ttagatgtta tcaaaaatgc
     7081 ttataactta ggcatggtta ataaagataa tatgctatta cgaaatgaag caattaatgc
     7141 ttatagaaat tctatttaaa taatgaaaac aatgcccagc ttaaataaag agctgggcat
     7201 tttacttatt ggggttattt gctttcaaat actctcgatg ttctttctcc aatttattaa
     7261 tatcatcttt taatatatag atcttatatt tttcctttgc ttctcttgac agattatgtt
     7321 ctaaatattc acggagcttt gtttcacttt tggtttgatc catgccaatt gtaattgctg
     7381 gatctgttat tccaatttga aaactaaata aaccaaattc atcccaagca ctttctaaaa
     7441 cttcttcagg tgcttttttg tttgattcga ttaaagtaat acccgatata atcaaagcag
     7501 gaactaagac caaaaatcca attaacctct tcttcattca ccatcacact ctccctcgtc
     7561 gaaaagaatc tctcctttat taatactacg aatatcatta ttgacctttt attttatttc
     7621 caacatttac atcgaacttt tgttctggtt tatcttgaat taagaacgtt tgttctgtta
     7681 atattagatc aataaaggag tgggtcgata tgcttagaga tcgaggaaca atcaaatgga
     7741 catcaatgat gcttccagaa catttaacac aacttaaaca agatttgatt gatgtgtcaa
     7801 aaattgaaaa accatcctta gatgaccaac aaattgaaga gatggatctt ctcgtctccg
     7861 aggcacttga atttaataaa gagttgagat ttaaactctt taacaatgga ttcgttgaaa
     7921 atgtcaccgg cagagtccat tacattaatt ttgaacaaca caggcttcat gtaaaagacc
     7981 agaatgacaa tacagtttat atcaacatga atgacatcat aggagttaca tacaatgatt
     8041 gattactcac aatttccacg aaagaatata ctttgtgtcg atatgaaatc cttttatgct
     8101 tctgtatcgg ctgtaacaag ggggcttaat cctttaacat gttatcttgc cgttgtaggg
     8161 aatacggaga gacagggaag tgtagtatta gctgcatctc ctgcacttaa aaaagatttt
     8221 ggaatcaaaa caggatcgag attgttcgag atacctgaag atccaagaat acacgttgta
     8281 aatccgcaaa tgaagctttt catcagagtt tcgactgaga ttacaaagct gttttacaga
     8341 tttgttcctg agaaatgtgt ccatacgtat tcaattgatg aatctttttt agatgcagga
     8401 aaagaaaatc ctgaagaaat ggccaaagca attcaaagta gcatgcggag agagtttggc
     8461 ctgatgtgta cagttggaat tggtgacaat atgttgctca gtaagcttgc acttgacctg
     8521 gagagtaaga aaacaaaaag tggtattgca cgttggagat atgaagatgt gccaaataaa
     8581 ctctggaagg ttcgcccttt gtctaaaatg tgggggatag gagggaggat ggaaagaaac
     8641 ctaaatcgga tgggaatatc gactataggt cagttagcca aatttccttt agagctgctt
     8701 gaaaagaagt tcggaataat gggaaaccag ttgtactacc atgctcacgg aattgattta
     8761 tcagaaatag gtgctccgtt gatgcaaggt cagattagtt tcggtaagag tcagatttta
     8821 ctgagggatt acacaaggag tgaagagatt aaggcggttc ttctggagat ttgtgaagaa
     8881 gtcgcaagaa gggcacgtac acataataaa gttggtcgaa caatcagtct gggaattggg
     8941 tacagtaagg atgagcttgg tggtggtttt catcgttcca aaacaattga tcttcctaca
     9001 agtatcacga tggatattta tagatgctgc ttgatgcttt ttaataagtt ttactcgggt
     9061 aagactgtga gaagtgtctc agtcacgtta tcgaatattg aggatgatgt taatcagcag
     9121 ctgagtttat ttgaagtgga taatgaaaag agaaggaaac tcggttttgt aatggatggg
     9181 attagaagta aatacggctc taaagcgatt ctgagagcag tttcttatac accagcagga
     9241 actgcacttc aacgagctgg attaacaggt gggcataaga gttaagataa atttaaactt
     9301 atataacaca tcgcttaaag tttttttgtt ttaaaaactt aaaaaacatg gtaaaattat
     9361 ataaaaacat aagaaagagt gattatatgg aatatgtagt tatgataatc attttattag
     9421 cacttttctt tatttttact gttttcctaa atacacgtta tagttttgat gaaaaatgct
     9481 tagtcttaaa atttggttta tctaaaacag aaattccaat taatcaaata gttagtatta
     9541 aagagtcaga caagtatgga gttgcagata atatcgatta taaaattggt atgccatatg
     9601 ctcaaccaga tagaattgtt attgaaacta caaataagcg ttttctagtt tttttaaatg
     9661 gagctcaaca atttattcaa aagtataaaa gggttagtgt ttgaacataa aaaagtacct
     9721 tcttacaata gaaggtactt ttttgtatct ataattatta aaaatttacc taaattttta
     9781 tcattattaa ttcaaaataa atccataata gtcaatttta tttagtgtat tacaaccaat
     9841 tctgtttatt gataggtaat aaagtttttt ttctatgatt tatgaacaag tttccttata
     9901 attctcaaaa aaaaataaaa aatatggttg aatttagatt tatcttcctt tatattaaaa
     9961 aatgtaatcc ggattgcaaa caaatgggga ggttttacaa atggaaaagc tatttaaaga
    10021 agttaaacta gaggaactcg aaaaccaaaa aggtagtgga ttaggaaaag ctcagtgtgc
    10081 tgcgttgtgg ctacaatgtg ctagtggcgg tacaattggt tgtggtggcg gagctgttgc
    10141 ttgtcaaaac tatcgtcaat tctgcagata aaacatttgt agagggaata ttttaaatat
    10201 tccctcatat ttaaagcggg gattgaaatt gaataagaaa aagaaatatg ttcatactaa
    10261 acagtttaat agtcatgatt gtggactagc ttgtatctcg tcaattttaa agtttcataa
    10321 ccttaactat ggaattgatt tcttactaga cctaattggg gataaggaag gctatagttt
    10381 aagagactta attgttattt ttaagaagat ggggataaaa actaggccac ttgaattgca
    10441 agaaaataag acattcgaag ccctaaaaca aataaagctc ccttgtatag ctttgttaga
    10501 aggggaggaa tatggacatt acataacaat atacgaaatt agaaataact atttacttgt
    10561 tagtgatcct gataaagaca aaataactaa aataaaaaaa gaggattttg aaagtaaatt
    10621 cacaaacttt atattagaaa ttgacaaaga gtcaattcct gaaaaagaaa aagatcaaaa
    10681 aaaacattct tactttttta aggacatact ttttagaaat aaattgatcg tttttgtgat
    10741 tttattgact tccttgttcg ttgtgggtct tgctgtagct gggtcgtttt atataaagtt
    10801 tctagttgac ctaattatcc caagaagctt aagagaatct ttaatcacaa tcactttaat
    10861 attcataagt atggtcttaa taaggtgcat cttcgatttt gtaagatcat atttgataat
    10921 aaaattgtct tacaaagttg ataaagagat gtcaaatgtt tattttaata aagtaacaaa
    10981 attacctatt aatttttttg aaaacagaga agatggagag gtaatttctc gattcaatga
    11041 tggtatatat attaaagact tttttagtgc taactttgtt actgcaataa ttgatataat
    11101 tttaatactg ggattaggag ttattttata tagaacaaat aacattcttt tcttaacaat
    11161 tattctcccg attttgctac tttcatgtct agcgattttg ttttttgatc atcttaaaaa
    11221 gaaaaatcaa aaactgatgg aggataaggc taaatctacc tctcttttaa ttaattttct
    11281 aaagaatatg acaactgttt attctttaaa taaaacctcg ttttttttag aaaaatttca
    11341 tcttacatat gataaacaat taaattcaac ctttagtgta gcaaaggcag ttattagcaa
    11401 tgaaatacta aaaggattaa ttcaaaactc ttttacaata attatcctat gggttgggac
    11461 aagacaagtt ctaaatgatt caatgagttt gggtacactg ctatttataa acacattagc
    11521 agcctttttg ctaagttcac tagatcgtat attgagtatg caatcagatc ttcagcaggc
    11581 acatgttgct tccataagat tttttgacgt agtaaactat ccagttcagc aagatagcaa
    11641 tgagaattta actgaacttg attttattca gaatatcaaa acagttaatc ttaatattgg
    11701 ggcagaccca atgcgttata tagttgaaga tattaattta atattagaca gaaaggataa
    11761 agtccttatt attggagaaa gtggtactgg aaaaagtacg tttgcaaaaa gtttgtctaa
    11821 attgtataaa gtacccgata agtcaattta tttaaatgga ttagatatta atcgatacga
    11881 tcatttatca ataagaaaaa ggattgtata tattgacgaa aatccttttc tttttaaggg
    11941 aactattaaa gagaatcttt gcatgggaga gatttttgat cagaacgaaa ttgaaaacgc
    12001 gtgtataatg tcccaatgcc atgaattcat ttgtaacttg gacaagcagt acagttataa
    12061 attgtctgaa aacggttcca atctatctac aggacaaaaa caacggttag cattagcaag
    12121 agcaatttta catcaaccac aagtattaat tttagatgag tcattatcta acattgatcc
    12181 ggataacaca aaattgattt atgaaacctt acacaggatg gattgtttaa ttattcttat
    12241 aactcataat gacccaagta acttcaaata caataaaaaa ttagtattca gaaacaatag
    12301 gattatagag tcgagctact cggaaaataa ggagtattct atatgaaaaa gtggattgtt
    12361 ttatttcttg ttttaatagc agcagccatt agtattttcg tttatgtttc tacaggtagc
    12421 gaaaaacctt tttataatga tataaattta actcaatatc aaaaagaagt agactctaaa
    12481 aaacctaaat ttatttatgt ttatgagaca agttgtcctc cttgtcaaga aataaaacct
    12541 gagttaaatg aagtaattaa aaaagaaaag ttaaaagtac aggctttaaa tattgaagaa
    12601 aaggaaaatt ataacactga atttttagat aaatataatt tgaataaaac tccaacgatt
    12661 ctctattaca aagatggcaa agaaaaagat cggttagagg gctatagaag tgcaagccaa
    12721 atagaaaagt tctttgataa aaatggtgat agataatgaa actgagtgat atttatttgg
    12781 aattaaagaa aggctatgcc gattctttat tgtattcaga tttgtcattg ttggttaata
    12841 taatggaata tgaaaaagat attgatgtga tgtcaattca atctttggtt gcaggttatg
    12901 aaaaatcaga tactcctaca ataacatgcg gtattatagt ttataacgaa agcaagagaa
    12961 ttaaaaagtg tttaaatagt gttaaagatg attttaacga gattattgtt ctagattcat
    13021 actccactga tgataccgtt gatattatta aatgtgattt tcctgatgtt gaaattaaat
    13081 atgaaaagtg gaagaatgat ttttcctatg ctagaaataa aattatagag tatgctactt
    13141 ccgaatggat ttattttatt gatgcagata atttatactc taaagaaaac aaagggaaaa
    13201 tagctaaagt agctagagtt ttagagtttt tttctattga ttgtgtagtt agtccatata
    13261 tagaagaata tactggacat ctatattctg atacacgaag aatgtttcgg ctcaatggta
    13321 aagttaaatt tcatgggaaa gtgcatgaag aacctatgaa ttataatcat agtctacctt
    13381 ttaatttcat tgtgaacctt aaggtttacc ataatggata taatccttca gagaataata
    13441 taaaatcaaa aacacgaagg aatataaatc tcacagaaga aatgttaaga ttggagcccg
    13501 aaaacccaaa atggttattc tttttcggca gagaactaca tttacttgat aaagatgaag
    13561 aagcaattga ttatctgaaa aaatcaataa acaactataa aaaatttaat gatcaaagac
    13621 attttataga tgctttagtg ctattatgta ctttattatt gcagagaaat aattatgttg
    13681 acttaacttt atatttggat atattggaaa ctgaatatcc aagatgtgtt gatgttgatt
    13741 actttagatc tgcaattttg ttagtagata tgcaaaataa acttacttct ttaagcaata
    13801 tgattgatga agctcttaca gacgagagat acagtgctat aaatacaaca aaagatcact
    13861 ttaaaagaat tttaataagc cttaatattc aactcgaaaa ttgggaaaga gtaaaagaaa
    13921 tatcagggga aattaaaaat gataatatga aaaaagaaat taaacaatat cttgccaact
    13981 cactccacaa tattgaacac gtcctgaaag gaattgaagt atgaatacaa gatatgtaaa
    14041 atcatttttt ttattactgt tttttctctc tttctttggc acaatggcta gtttattcta
    14101 cagtgagatc atgcatttca aaccatgtgt tctatgttgg tatcaaagaa tatttctata
    14161 tcctatacct attatcttac taataggctt attaaaaaaa gatcttaatt cgatatttta
    14221 tgttgttttc ctttcatcaa ttggattgat tattgcgttt tatcattata ttatccaact
    14281 tacacaaagc aaaagtgtcg tatgtgaaat tggaaccaac agctgcgcaa aaattgaagt
    14341 agagtatcta ggctttatta cattaccctt aatgagttca gtatgttttg cattgatatt
    14401 tggtatagga ctgaaattaa ttatcaaaag caagaaatta aaacaaaatc aacatgtata
    14461 taattgaata agaagtaacc cgccttgtaa gagggagggt tacacaggta atccgaaaaa
    14521 agctatttaa acaagttctc tttttttaga acttgttttt gtttttttcc ttttgcagtt
    14581 atgtaattgt ttttgaacca tgctgcggta gtagtactgg ttgtgaagac gatagtgaaa
    14641 attgtatata aacaatcata taaatgattt atgtcctctt cgttaatgat gaatgctgct
    14701 ttacccagca tcaccattat ttgattgagt aaagctattg cgagcaaaag agtcctaaca
    14761 attgtgcctt tatcaatatt ctcaaacatt gaattcctcc tacttaaaat tttgttcgat
    14821 tttatctagc ttttcaatta caacatcgta cttttcactg aatttaccca gtacttcgtt
    14881 ttgagaatcg atttgattat aaagtttcga ctctctttcc ttactagttt tcattacgta
    14941 gaaaagtagc caacaaaata aaacagcaaa tggcccctgg gtacttaaat attgtgttat
    15001 atccatttcc ataaaatgtt caccacctta gtaccataga aaaggatatt tactgaaaca
    15061 tattgatcaa taaacatcct ccgagaaaat tgaatataat tagattgttg atccaaactg
    15121 tcccgagata tatccacgtt tacctttata aataacttcc cagtagccat tggaattgtt
    15181 tttaccttta actgaaccag aaattgaaat agtgcttcca agcttaactg tgccaatatt
    15241 tttagaacta tttcgatcag gcttgtccat tacgattgca gcgcttgata caccgacaat
    15301 tttgattttt cctacggatt taatagatga ggaacttgaa gaagttgatg ttgtttttgg
    15361 ggcagaggaa gaggttttaa ctgatcctgt aacatcaaca tatttgtcag aagcagtgat
    15421 gtaatatgta gtgccttttg agttcttaac tttgtattga taggcagatc caactttaac
    15481 tttctctaca actgtaggaa atccgatgcc tttatttact gtgccgacaa catctttatc
    15541 ttcccaagat ggttttgaat agaagcgaag tccgtcaact tttgatttta gtgaaccact
    15601 tactgctgat gaagaggaag gggaggaggt tgttggttta gttggagaag cttttgaaac
    15661 agatttgcct gacatttttg cctttactcg atttttaaag tctacaaatt tttggctgtt
    15721 agatacccat ggtgcagggc aattcttatg ggtgatgtca taatgacgaa caatatcatc
    15781 aataggatct aaaccaaact tattacataa ttcaacgaac acatcctcag ttctttcaac
    15841 tgtatctgaa tggaatgaac cgtctttttc aaggcacatt tcaactccaa tagaaagaaa
    15901 gttagcgtta ggtttcagcg cagcaactcc tcgataagga tttccggcac tatctctttg
    15961 ctgaatatca tttgcatggt aagctacttc atttaatgga atgatacaaa ttgcttcagc
    16021 cttatctacg aaaatgtgag ctgatgcata aacttgtgca ttactgaagt atcttcgatg
    16081 attgtctgca cctgcacccg gattggctgt atagtgtagg acgcattttt tcacaccctg
    16141 taatttcaaa cctggtctag tatacttatt gactggaatg tagctattag tgaaaactga
    16201 cataaaaaca tctccttaat ttctaattaa aaagagcctc gaattcacca ttttcatagc
    16261 gatttcgaaa ctcgttgaat tgaatctcat tattgttgga tgttccataa atttcatgga
    16321 aaagattgtg gattttctta gttagaggga ttccgagtcc ataatttaaa tgaagtctta
    16381 cacattcatt tttaatttgc tcaagctcat tgacagtaaa gtcgccaatt ttagactgca
    16441 gttcaaaact taaattgctc agtgcttctt taacaatctc gctaaaattc tttttatagt
    16501 ggtgcacatg aaggtctttt gtagagtttg ttattgcaca tttaaatgaa tatttctcta
    16561 atgagggctt cgtccaaact tcataaacag cttctcttaa agttttattt aacgaagaaa
    16621 taccgccttt ccatctagag ctattttcgc ctgtgaaaag aagtaaataa cactttctgc
    16681 agtttgctaa acctcttacc atgttatgaa gtggagcgta ttgaactcct aagtctatat
    16741 gttccttgca tagaaaaggg agattttctt tattgtgatg gtatgtatcg aataaaggga
    16801 taaacccacg atcagaaaaa gctgcttgtg cacattcaac agtgtatttt ttagcacctt
    16861 tacattgaaa acactcttca cctttaaaga agttgctggg aaggtgctcc catatatgtc
    16921 cttcagggca cttagctttt agtttagaat gacagttttt ataggtgctt aaaatctcat
    16981 agcctttaag ggcatatcta cttttcacag tacttatgtt aagccttttt ttaagggagc
    17041 cttcaattat cctgcattct ggacaacgag tacctttttt aaagtgttcg tagtttactt
    17101 gccatgtgtg attatgtcga catctgactt tcacctttgt tttcgaattt accatgtctc
    17161 cagaaatgtg aatgtagtta ttctgagcta ataaatcaat aaatttcttt tgagtttctt
    17221 cacgtttttt taaatgataa tctgatatct ttcttgccat ctatccacct aataattaaa
    17281 tttgagcaca aaaaagagag aaggggacta aacccaatct ctctttatct cacgtatgct
    17341 cttgttatct ctgtttttca agtatgtatt ttgaataaaa tctatatttt atctagaacg
    17401 taaacacctc cttaattttt aatgcattta gattgtaggg gtgataagac atagcgctac
    17461 accatatcct cgttcttgtg aatacggggt tgtgatacgc attacatagt aacctgaacc
    17521 atcctcagat tttgaccctt taccggcttt aggaattata cgatcaccag ctgcaacagt
    17581 cccatcaata cgtacaaata tctggccaaa cattcctacg acattccatt caggccgatc
    17641 agaccgagca atataatctt cagcaggttc ataataagga ttttctttcg gaactgtttt
    17701 agtttcgatt ctttgtgtac cgtcttcatt tgtaactgtt acatcaatat cttcgtaaat
    17761 caaaccgcca aattcgttct tcaaataacg gccttgccag ttaaatgctg cttcgcctaa
    17821 aactacacca gctgtttcag aaatcactcc taaaacttta tctcctgcgt tggcttttct
    17881 gattttgtca ccttctagag ttacaaaata accagaagga atggctttac catcaatcga
    17941 ttcaaaatac tcggcgtaat ctgagaaagt tgaggcacct ttaacttgac cggtaagatt
    18001 aatattccca ttctttgcat ctaattgaat ctttgtattt gctgtagagg gtgcacctgt
    18061 agcagcatat cctaaagcaa ctgtgtaacc tgttttattg gcaacacctt gggaagctat
    18121 gaccatccta gatgatccag tgccttcaat tgaagagtta tttgaagcgg caaccattga
    18181 acgactgcct tcagatgtgg ttttagaacc gccagatgaa gcgattacta cgttacgagg
    18241 gccatcggct ttagatccac cagttgttgc tataactgca gaagcagtac ccgttgcttg
    18301 tccaccacca gaagaagcag ctacaagact agtattttta gcagcgtagc cagaagttgt
    18361 cgcaatcctt gtgccacctt taatattttt tggtacaaag gatgtatagg tttttccggc
    18421 aatcttcgag gggatagcgt atttatcaca attgactgcg ttaatgtcaa cttgagagtt
    18481 tgtacaatac aagccaatag acccttcttt gctgtaacca attaaacttg cttgatttac
    18541 actagcgttc ttaatagcac tgccaattct gatgccaatt aatgcagact caagaacatt
    18601 tatatttgat aaggacacaa agtcagattt ttgacttcct ccataaatat aaatatctgc
    18661 tccagctgtt ttaaatccag atgaagaaac attattaaaa gtaatgttcc tgcttttata
    18721 ttgagttgca atagctggat tacctttata gtcataagaa gggtcaccga tagctgtgaa
    18781 attagaaatg ttaacatttt tgtaagcaga aataaccaaa gctcttggtg tcattccagc
    18841 gtataaccta tcgttgaaaa ttggttttat tgctgtgcag ttagaagcca taatatttct
    18901 tgcagtttta gattctgggt cactagcttt atggaatcca atgtgtctaa aatcaaaact
    18961 tcgaatatca ttttcagata cacaatttaa aaaggtgaca ttctgtgctg ctggtgcatg
    19021 ttcatgagct ttagcttcaa atcctcggca gttattctta ctgacacagt tgatcagcca
    19081 cactttcatt gaaccatcat caatttcaaa tccatttgtg ttgctggcgc ctgatgtatg
    19141 agctgatccg tttccatcat atgaataaca atttgttata aaaacatact ctgagaagtg
    19201 ggttgtgaaa ccatcgtcgc caaagttcgt tgctgtacag ttattaatcc aaacgtattt
    19261 acttccacct ggttgataaa catcttctgt atcagtaagg gagttataag aaggagaggt
    19321 tacatcaaac ccgtgtaatc ctgcatcttt tgcatgaaca ttatttaccc atacaaattg
    19381 tgaattggta atgtttaagc aacttgagtt ggggccagag gaaatgctgt tgttagtctt
    19441 ctgaaggttc cagtcaagca agaggttttc aaaaatgatg cactttgaat tttgaacagt
    19501 tgtgatgacg tggctgtatg cgggggcatc tttatgaagt ttaataactg agagtttacc
    19561 tgttccatat aatctagtat aggatggaac tttcaatcct ttaaccatat atgttccagc
    19621 tggcacaaat acttctgcat atcccatttc aaaagctttg ttaaaggctg cagtgtcatc
    19681 agtcacacca tcgcctttag ctccaaaatc cgtaacgcta actctacgct gaattaatgc
    19741 atttaatatc ttctcgacaa ggtttgtacc cgtaatatga aaatcatcta tgttcccaat
    19801 ttgattagat agtccatcaa ctttctcagt gacgctactt gaaacactgc ttacttgttt
    19861 taggatatcc tcgaaagttt gcttaattga tggcgcagtg taagaaacgc catctgattt
    19921 aagcaattca atcgacatgg gaacatagga accagtattt ttatcataat atttaaatcc
    19981 catctaattc ctccaaatta aaaaagagac tagtagattc tctttctaat caatttcata
    20041 ccaaaccata cctgattcag gtgcaacatt tgatatgatt actctttgtt tttcaggtac
    20101 caagccttca tcttgatacc atatataatt tgtactaaac ggttcgacag cacttaataa
    20161 aatattgaaa c
//
