LOCUS       NZ_CP093291            10312 bp    DNA     circular CON 24-MAR-2024
DEFINITION  Bacillus mycoides strain DSM 2048 chromosome, complete genome.
ACCESSION   NZ_CP093291
VERSION     NZ_CP093291.1
KEYWORDS    RefSeq.
SOURCE      Bacillus mycoides (Bacillus weihenstephanensis)
  ORGANISM  Bacillus mycoides
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 5285600)
  AUTHORS   Chiba,A., Peine,M., Kublik,S., Baum,C., Schloter,M. and Schulz,S.
  TITLE     Complete genome sequence of Bacillus mycoides, isolated from an
            agricultural field in Rostock, Germany
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5285600)
  AUTHORS   Chiba,A., Peine,M., Kublik,S., Baum,C., Schloter,M. and Schulz,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2022) Research Unit Comparative Microbiome
            Analysis, Helmholtz Zentrum Muenchen, Ingolstaedter Landstr.1,
            Neuherberg 85764, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 15-MAR-2021
            Assembly Method                   :: HGAP v. 4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 1166.0x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022630575.1-RS_2024_03_24
            Annotation Date                   :: 03/24/2024 09:23:57
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,704
            CDSs (total)                      :: 5,549
            Genes (coding)                    :: 5,305
            CDSs (with protein)               :: 5,305
            Genes (RNA)                       :: 155
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 108
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 244
            CDSs (without protein)            :: 244
            Pseudo Genes (ambiguous residues) :: 0 of 244
            Pseudo Genes (frameshifted)       :: 93 of 244
            Pseudo Genes (incomplete)         :: 175 of 244
            Pseudo Genes (internal stop)      :: 63 of 244
            Pseudo Genes (multiple problems)  :: 79 of 244
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 07:31:00
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4898862
            Orig. end                         :: 4909174
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP093291.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Research Unit Comparative
            Microbiome Analysis, German Research Center for Environmental
            Health, Ingolstaedter Landstr. 1 85764 Neuherberg.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10312
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="11"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10312
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10312
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5312](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5312
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    396..411
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: strong, score: 18.39"
                     /tool="antismash"
     misc_feature    complement(899..914)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 17.4"
                     /tool="antismash"
     misc_feature    910..922
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 20.68"
                     /tool="antismash"
     gene            <939..1076
                     /locus_tag="MN093_RS25475"
                     /old_locus_tag="MN093_25480"
                     /pseudo=""
     CDS             <939..1076
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002203238.1"
                     /locus_tag="MN093_RS25475"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="MN093_25480"
                     /product="YolD-like family protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="YHEDGYLLTSYMTIVDIDLLNSAVICTDSFYNKMKLQFSNIIDVK
                     "
     gene            complement(1437..>1681)
                     /locus_tag="MN093_RS25480"
                     /old_locus_tag="MN093_25485"
                     /pseudo=""
     CDS             complement(1437..>1681)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016126852.1"
                     /locus_tag="MN093_RS25480"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /old_locus_tag="MN093_25485"
                     /product="hypothetical protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="NNLNIGKRVVSKVDNLCFYDSPY"
     gene            1880..2203
                     /locus_tag="MN093_RS25485"
                     /old_locus_tag="MN093_25490"
     CDS             1880..2203
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653290.1"
                     /locus_tag="MN093_RS25485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MN093_25490"
                     /product="hypothetical protein"
                     /protein_id="WP_215576463.1"
                     /transl_table=11
                     /translation="MKDKILELVPRSVKKLLQKKVKDGNRCIIDLVVDDATAYGFEPEQ
                     VIPCIMAGYYVYESTTYPSEMEEEWKLNLVFHAANRSAMKLLSYGKYKAKQSESGWMRY
                     TNP"
     gene            complement(2345..2845)
                     /locus_tag="MN093_RS25490"
                     /old_locus_tag="MN093_25495"
     CDS             complement(2345..2845)
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1250: DinB"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002188491.1"
                     /locus_tag="MN093_RS25490"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MN093_25495"
                     /product="DinB family protein"
                     /protein_id="WP_131245500.1"
                     /transl_table=11
                     /translation="MANHVENLYNYHVWANQRIIDHLKTLSPEKYQKEMKSVFSSVSKV
                     VSHLYLVEYGWLNTLEGQSMNEAMQSSFQIQEKIEKLPIEDLEMEFHTLTSRFKTFLNR
                     QEDMEKRIMLDNPWAGLRETSISEMVLHVVNHGTYHRGNISAMLHQLGDSSVMTDYAFY
                     WYS"
     gene            <3457..3567
                     /locus_tag="MN093_RS25495"
                     /old_locus_tag="MN093_25500"
                     /pseudo=""
     CDS             <3457..3567
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002148758.1"
                     /locus_tag="MN093_RS25495"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="MN093_25500"
                     /product="alpha/beta hydrolase"
                     /pseudo=""
                     /transl_table=11
                     /translation="VIISKINHRISLANPELFNSLVENWIEHDALPKEVN"
     gene            3642..4295
                     /locus_tag="MN093_RS25500"
                     /old_locus_tag="MN093_25505"
     CDS             3642..4295
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080302.1"
                     /locus_tag="MN093_RS25500"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MN093_25505"
                     /product="DUF2306 domain-containing protein"
                     /protein_id="WP_131245499.1"
                     /transl_table=11
                     /translation="MNKKTWFIFTFSAILVASYAVVQYFIMDGFQAGFVQMKLMFLSKM
                     SAFWYIMLFIHIAASVVALVIGPFTLSTKFREKNISRHQIIGKIYIIGILFGGVSGLYL
                     SFYATGGLVGKLGFGLLSVFWLTSAYQALNRVKNKKIQDHRNWMIRNYSLTFAAVTLRI
                     WLPLFVLLFGLKHFELSYAVIAWLAWVPNLIIAELFIRQSLNKGQQQKNADLHF"
     gene            4446..4556
                     /locus_tag="MN093_RS25505"
                     /old_locus_tag="MN093_25510"
     CDS             4446..4556
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000505686.1"
                     /locus_tag="MN093_RS25505"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MN093_25510"
                     /product="YjcZ family sporulation protein"
                     /protein_id="WP_000505681.1"
                     /transl_table=11
                     /translation="MGFGGSCGGGCGFAGGFALLVVLFILLIIVGASCFC"
     gene            5001..5312
                     /locus_tag="MN093_RS25510"
                     /old_locus_tag="MN093_25515"
     CDS             5001..5312
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03601"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002059557.1"
                     /locus_tag="MN093_RS25510"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MN093_25515"
                     /product="heterocycloanthracin/sonorensin family
                     bacteriocin"
                     /protein_id="WP_215576464.1"
                     /sec_met_domain="TIGR03601 (E-value: 3.4e-36, bitscore:
                     121.8, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKMNQFQQELQALSLNDYQSENVVYWDQQNQYPYYYIEGDARRCG
                     GCGRCGGCGGRCGGGGFRCFGCFGCFNCGGCGGCGGCSNCFDGFNGFNGFNGTTVTFE"
     gene            complement(5882..6145)
                     /locus_tag="MN093_RS25515"
                     /old_locus_tag="MN093_25520"
     CDS             complement(5882..6145)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="MN093_RS25515"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="MN093_25520"
                     /product="hypothetical protein"
                     /protein_id="WP_234879430.1"
                     /transl_table=11
                     /translation="MLKRNAKYILGENSEATSKYSLFLGSYVKLGAMMGMPFLGIANPK
                     VFLASKPFGKEPDLYENENVEVIYLLRLMNAIFVLVCWKENK"
     gene            complement(6570..7418)
                     /locus_tag="MN093_RS25520"
                     /old_locus_tag="MN093_25525"
     CDS             complement(6570..7418)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078040.1"
                     /locus_tag="MN093_RS25520"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MN093_25525"
                     /product="DUF2935 domain-containing protein"
                     /protein_id="WP_131245497.1"
                     /transl_table=11
                     /translation="MNRDETSLHPDTGVTSVMFVERSLNEIRFWSRIMKEHSFFLRLGF
                     RCEDTQLIEEANQFYRLFEHIEQIAYSYTNETDPGQIKRFNSEVQQAATNIWGFKRKIL
                     GLILTCKLPGQNNFPLLVDHTSREANYFRKRLIELNEGKLDALPDAIIKENVFFLRIMA
                     DHAKFIGHLLDPSERKLVDTARNFSNDFDELMYQAIDLESMKPQSQTVPLLDQFLDQNR
                     VSVTSLRDFKKTARDLIEQCKIKSIIHPLLADHVFREADRFLEIIDMFDAHLTNIKSQS
                     R"
     misc_feature    complement(7532..7552)
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 25.31"
                     /tool="antismash"
     gene            complement(7589..7732)
                     /locus_tag="MN093_RS25525"
                     /old_locus_tag="MN093_25530"
     CDS             complement(7589..7732)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="MN093_RS25525"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="MN093_25530"
                     /product="hypothetical protein"
                     /protein_id="WP_165497884.1"
                     /transl_table=11
                     /translation="MESIEYIAIGSRGIVKNNLNVYIGVVAHRYQAKVAPGKLQKKSSL
                     LI"
     gene            complement(7800..8720)
                     /locus_tag="MN093_RS25530"
                     /old_locus_tag="MN093_25535"
     CDS             complement(7800..8720)
                     /EC_number="2.1.1.-"
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000337841.1"
                     /locus_tag="MN093_RS25530"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MN093_25535"
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="WP_215577151.1"
                     /transl_table=11
                     /translation="MCVKQGEASVTSLVSAFGRAYHSEFESPKIFDDYIAKDLILQKER
                     NNIEMNMVQGIHFFNKEIAEQFQDNPKEILKWITQVQLSPTPLARAAYCERVLLHEITL
                     GAKQYVILGAGLDTFSFRHRELENKIEIFEVDHPSTQQFKKIRVKEAELEIPNNLHFVS
                     MDFTKGFSYEQLRNEGFENKKTFFSLLGVTYYLTKEELSSLIECLFESVPAGSSIVFDY
                     PDENLFTEKGLSNRVENMVKMAAVGGEPMKSCFSYTEMESLLEKSGLLIYEHLSPEDIH
                     TLYFEGRNDYLEAFETVHYVHAVKR"
     gene            complement(8909..9382)
                     /locus_tag="MN093_RS25535"
                     /old_locus_tag="MN093_25540"
     CDS             complement(8909..9382)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002128099.1"
                     /locus_tag="MN093_RS25535"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MN093_25540"
                     /product="DUF4188 domain-containing protein"
                     /protein_id="WP_215576465.1"
                     /transl_table=11
                     /translation="MGAKIFNGRHIANSKDQEFVVFIIGMRINNLLKFWKWIPVFNAMG
                     PMIKELYQNPQWGFLHTEFLFSWRKVILIQYWKGFDELVNYAHGKNHSYAWKSYNNKIK
                     DNGSVGVFHETYQIEKGASEAIYVNMPKTGLSRATEHIPVSQDTNTAQKRMGK"
ORIGIN
        1 caccttcaca agaatgaagc aatttcggtt cgttagaacc ttatatgcac ctgtctgcta
       61 gtaggcgttg atgacagttt cttgcttacc acgttccaat tgacttagct atacatatat
      121 ccttagatgc ttgctttaag cctgttatct ttacaccttc atcgttagat gttccgaatt
      181 tcatatcatt tactcttact tttcggtaga taacgtcgtc attgacgaca tacacatttc
      241 taatgtatta agcgtttaga cccgtacatt cgcaagttcg ccagtccatc tggacattct
      301 caccatagat attttgtagc atcccgacct atccatcacc atatgatttt tcacttaggt
      361 tttgttcagc ccacttcacc tagcttcata caatttgtga ctactatcac gccttgcatg
      421 taggagtatc agataagtag tttcagctca acctgacggc tttcattcct actttcagtc
      481 aatcagttgt tgttatcaag ttgataaccc tcctatttcg tgtttgtaca cttatgagga
      541 aacgtgtcgc acaaataacg cttttatatg aaagtcgtac acatttttga cacatcaacc
      601 atcacaatta cttgaaaact cgtgataata ttaattcgtc gaatacacca aatttcgaca
      661 ccgtacgaca ataatatctc tcctataaaa tccctgtccc cgcagggatt ttctctttta
      721 ttcattactt tttgttgcac tgaattccca tcctgaataa aataacttaa catggtaaaa
      781 tatcatttgg atgggagtcc aatacatatt attaaaatta agttggttca agtcggagga
      841 aggcacctta gggtgtcttt ttttacggta aggcattaat gttttctatg tctcgattag
      901 tgaataattt cacattttgc aataacatca aaagctttta tcatgaagac ggttatttac
      961 tcactagcta tatgactatt gttgatattg atctgctaaa tagtgctgtg atatgcacgg
     1021 attcttttta taacaagatg aaactacagt tttcaaatat aatcgacgta aaataaaatt
     1081 cacccaacaa aaaagaaaag gagaaacctc ctcttttctt tttaatttaa acttagaact
     1141 aagtgtagcc ttgaaataaa gtttgattaa attaacttaa taaactttga taaaagagca
     1201 aataaaaaaa gcgtggttcg aaaaaagatt gttcaaaaca cgctttttct atattcaatt
     1261 aaatcatcct tttataaaaa agtttgataa tctttgtgat cattccttca ttaaagcgcc
     1321 cgattgtgga ataagaaaaa atcctgcaca ttaacacagg atttttaatc aaactttttt
     1381 ataaattatc aaactttgtt ttatggccac aaagtgacat ctaaaagtta gacacgttat
     1441 ttcattaggc agcttcttgg gcatgggccc ggtattgtac cgggctcatg ccttttagtt
     1501 ttgcctttgc ttttgtgtat tttatattgt ggatccattg acagttactt ttgcgcctat
     1561 agtaaatctt aatcctgcat ctacagatgc ttacaacatt attatcctgc taatatggag
     1621 aatcatagaa acataggttg tccactttag acacgacacg ttttcctata tttaaattgt
     1681 ttcgttccaa tcgactgcaa aatgaacgaa tacgtttttc cctgtctcac cagatttctt
     1741 ggatttaaag ccattttccc ttttagggtg atatagtcct taagatcttg ttaaacctgt
     1801 gcaattacgg ttggaatttc attctgtttg acacatatcc ccaggctatc tggacatgat
     1861 aaaagatgag gtgactgaca tgaaggataa aatattagaa ttagtaccaa gatcggtaaa
     1921 aaaattactc cagaaaaaag ttaaagatgg aaaccgatgc atcattgatt tggttgtgga
     1981 tgatgcaact gcttatggat ttgaaccgga gcaagtaatc ccctgtatca tggcgggtta
     2041 ttatgtttat gaatccacca cctatcccag tgaaatggag gaagaatgga agctgaactt
     2101 ggtttttcat gcggccaatc gctcggcaat gaagcttctg tcttatggga agtataaagc
     2161 gaaacaatct gaaagtggat ggatgagata tacaaatcca taaggtttaa ttaacataca
     2221 ctaactagct tggtgagcat gaggcaccgt cacatgccca tcaacttgag atcttgaaat
     2281 acccccaagt attttggtaa aaaatggatt actcttctct tgcctattta taatgaataa
     2341 catttcaaga ataccaataa aaagcataat cagtcataac tgaagaatca cccaactgat
     2401 gtaacatagc tgaaatattc cctctatgat aggttccatg attcacaacg tgtaagacca
     2461 tttcagatat acttgtttct cttaatcctg cccatggatt atccaacatt attctctttt
     2521 ccatatcttc ctgtctgttc aaaaaggttt taaaccgaga tgttaaagtg tgaaattcca
     2581 tttctaagtc ttcaatgggc agtttctcaa tcttttcttg aatttgaaat gaagattgca
     2641 ttgcttcatt catactctga ccctcgaggg tattaagcca tccatattcc accaaataga
     2701 gatgagatac aacctttgaa acagaagaaa agacactttt catttccttt tgatatttct
     2761 caggagaaag tgtcttaaga tgatcaataa tccgttgatt tgcccataca tgatagttat
     2821 atagattttc tacatgattt gccatacaat cgcctcctta tcatcacgtt ttttaatatt
     2881 ctttatcaga atgaattatt cctttaaata ttattacttg tctgatgaga tcttcaacta
     2941 aatggcccga ttgctgaata agaaaaaatc ctgcacatca acacaggatt ttgatcaaac
     3001 ttttttacat attatcaatc tttattttaa acgatcagac ttttttataa attattaaac
     3061 tttgttttaa agccacactt tttacaattt aggtcttgat aactttaaaa cggttaaact
     3121 ttatttcaaa gccacaggaa tctctccgtg gagataatgg gaaatgctgt atttaagctt
     3181 aggaatttcg ttttgggggc aacctatttt gttaggctga tggcgatgag gtggtacccc
     3241 taaacaagca attatctggt gtcgatttac atttaaggtt gccccatcca tatttgcata
     3301 agatgggcct gctgcttgca taactattgc ggtcattgct ggaaccgtga cacctactcc
     3361 taaattgatt attgagaaaa taattgctaa taacaaacat ggcacagatg gataaattaa
     3421 ccagaacttc cttttttgtt gtatgattaa ttaagtgtta ttatttctaa aattaatcac
     3481 agaatttctt tagcaaatcc tgaactgttt aatagcttag tcgaaaactg gattgaacat
     3541 gatgccctgc cgaaagaagt taattaagtt ctcttttaac cctagataaa cagcaatata
     3601 tgttctaatt cccttaaatg ttgcaaggag gtggtgaaat tatgaataaa aaaacttggt
     3661 ttatatttac tttttcagct attttagtag ctagttatgc tgttgtccaa tattttatta
     3721 tggatggatt tcaagcaggg tttgttcaaa tgaagcttat gtttctatct aaaatgagtg
     3781 cgttttggta cattatgtta tttatacata ttgcagctag tgtagtagca ttagtaattg
     3841 gtccttttac attatccaca aaatttagag aaaagaatat cagtcgccat caaataatcg
     3901 gaaaaatcta tataatcggt atattatttg gcggcgtttc aggactttat ttatcctttt
     3961 acgctacggg aggattggta ggaaagcttg gtttcggttt gttatccgta ttttggctaa
     4021 catcggcata tcaagcatta aacagagtta agaataaaaa aattcaagac catcggaatt
     4081 ggatgatcag aaattattcc ttaacctttg cggcagttac attaagaatt tggctacctt
     4141 tatttgtatt gctatttgga ctgaagcatt tcgaacttag ttatgctgtt attgcttggt
     4201 tagcgtgggt gccaaattta atcattgcag aattatttat aagacaaagt cttaataaag
     4261 gacaacaaca gaaaaatgct gatttacatt tttgatgcaa tttatacgta actcggctga
     4321 atcccttagg gtgtcttttc aaaaggacct gctcaattaa tgcgtaaaac cataaagtaa
     4381 aatgaatccg ttaaaataca tgagtgacct cttgttttat cctcttatct aaaggaggaa
     4441 ctattatggg ctttggtggt agttgcggcg gaggctgcgg ttttgctgga ggatttgctt
     4501 tactagtcgt attgtttatt ttattaatca tagttggagc ttcttgcttc tgctaaacaa
     4561 gctatcggaa aggacactct tatatgggtg tcttttcttt atggaaattt ctggaattac
     4621 ttatatatgg gtgtcttttc tttatgcatt aaaaagccca cctatttccg taggtagact
     4681 atatgtgatt ttgtacttgt gcacgattga attatagaag aaactcctaa tcttcgttat
     4741 acctctacag taataatttt acgtactata caactgcaca ttcagaagac aataatgttg
     4801 aggcattttc atcttaaaaa aatgagccca tagtaaaaac aaccttaaaa taggcatatg
     4861 aacaaggtgc atcatctagt gataggtcat attttgttgg tgtacgtatt gaacttagtt
     4921 tatagcgcta tttctactta gatttaacga acagaaatat aggaaagcat cgatggggtt
     4981 ttagtttata aacggaggtc atgaaaatga atcagtttca acaagaacta caagcgttaa
     5041 gccttaatga ttatcagtct gaaaatgttg tgtattggga ccagcaaaac caatatccat
     5101 attactatat tgaaggcgat gctcgtcgct gtggcggttg tggtcgttgt ggcggttgtg
     5161 gtggtcgttg tggtggtggt ggattccgtt gttttggttg cttcggttgc tttaattgtg
     5221 gcggttgcgg tggttgcggt ggttgctcta attgttttga tggttttaat ggttttaatg
     5281 gttttaatgg tactactgta acatttgaat aaagtttgat tagacatgtt ttaaaaatcc
     5341 ttatttagtg agaaccgaaa agaattcatt ttgaataaaa tttgaacaaa aacagagtaa
     5401 ttattgtgga ttagtgtaca aatttcacaa aaaagtccga ttaaaagttt tcaagaaatt
     5461 tgctacagca atattttctt taaaaatccc ctcatggtac gactttatta taaacatcgc
     5521 atcttttttc aaagcatcgt gactttattt caaacccaca tatttaacgg atataaagta
     5581 gatctatcag acttaccttt atataatgaa gaatttttct aagtgttttt ccaggcgtgt
     5641 aaaataagat ggcgtgtaac tttataaaat ataaaaacaa tagaatactt tattaagaag
     5701 caggatattc taaaacaatc caaagtgcat cgttttcata ctgtgcccgc tttgaagata
     5761 ctaaataatg atgcgaacta ttaataaaat cagtagctcg gactatcact gcggaatctt
     5821 gatcctcctt taatattgta tgaggtaata gcctagctaa atcctcttcc tcagaggaag
     5881 gtcatttgtt ttctttccaa caaactagca caaaaattgc attcattaat cttaaaagat
     5941 atattacttc tacattttca ttctcatata aatcgggttc tttcccaaat ggtttacttg
     6001 ctaaaaaaac tttcggattc gctatcccta aaaacggcat acccatcata gcgcctaact
     6061 ttacatacga acccaagaac aagctatatt tacttgttgc ttctgaattt tctcctagaa
     6121 tatatttcgc attccgcttc aacaaattag aacttttccc ctttctcata ccatagtata
     6181 agattttcat tacctacttg cttaacacaa gatgattata ctcaatactt atttcttctt
     6241 caggcgaaat atcttcagaa aaatatttaa ggataaatgc atttccataa tcatctaatt
     6301 tcgagagtac catgttgtta caattctcgc acatatcttt aacaacttgc ttttttggtg
     6361 ataacggtat tgccttcccg tcaattctcc taatatcctc aatttataaa aagaaattat
     6421 acattaaaaa gactataaac aaaggggtta aaaatccctt tgggaatatt taataaaata
     6481 acgtatgaat ccatcacaag gaaaagaagt taacaataaa ttgttaactt ctcagcttaa
     6541 agccaaatag tatctttcct tatcttctac tatctagatt gtgatttaat atttgtaaga
     6601 tgagcatcga acatatcaat tatttcaaga aatctatcag cttcacgaaa aacatggtct
     6661 gctaataatg gatggatgat actctttatt ttacattgct caattaaatc acgtgccgtt
     6721 ttcttaaaat cccgaagaga tgtgactgac acacgatttt gatctaaaaa ttgatctaaa
     6781 agaggaactg tttgagattg tggtttcata gattctaagt caattgcttg atacatcaat
     6841 tcatcaaaat cattactaaa attccgggct gtatctacaa gctttctttc cgatggatca
     6901 agaagatgac caataaattt agcatggtct gccataattc ttaaaaagaa aacattttct
     6961 ttaataatag catcaggaag ggcatctaat ttaccttcat ttaattcaat taaacgtttt
     7021 ctaaaataat tagcttccct acttgtatgg tcaactaaca gtggaaaatt attttgtcct
     7081 ggcaatttac atgtgagaat taatcctaga attttccgtt taaatcccca aatattagtt
     7141 gcagcttgtt gtacttctga attaaatctt tttatttgcc caggatctgt ttcatttgta
     7201 taggaatacg ctatttgttc gatatgttca aacaatcgat aaaattgatt agcctcttcg
     7261 attagttggg tatcctcgca tctaaacccc aatcgaagaa aaaaagaatg ctccttcatg
     7321 attctagacc aaaaacgaat ttcatttaat gatcgttcaa caaacattac agacgtaaca
     7381 cccgtatcag gatgtaatga ggtttcatcc ctattcattc ttaatccccc tcagtaaata
     7441 ctaagctagt tccattctta tgagtaaata tatatataaa taccatcaat aaaatttcat
     7501 gatgaataat gataaatctc gtatacatgt aaaattaacc taagctatca ttatcgatag
     7561 cccaaaaaag ctcactcctt atctattttt atataaggag tgagcttttc ttctgaagtt
     7621 ttcctggcgc aaccttagct tgatagcgat gtgcaactac ccctatataa acatttaaat
     7681 tattcttcac aatcccccta cttccaatag caatatactc aatactttcc atcactacac
     7741 ctcatttcct tttttcataa aaaaagacac agagattcct ccccatgcct actaatcccc
     7801 tacctcttca ccgcatgcac ataatgcact gtctcaaaag cttctaaata atcatttctt
     7861 ccttcaaaat ataatgtatg aatgtcctct ggtgataagt gctcataaat gagtaagccc
     7921 gacttttcta acagagattc catttcggta taagaaaaac atgatttcat cggttctccg
     7981 cctaccgcag ccatttttac catgttttcg actcgattag acaatccttt ttccgtaaat
     8041 aagttttcat cagggtaatc gaaaacgatg gaactccctg ctggaaccga ctcaaataaa
     8101 cattctatta aactggaaag ttcctctttc gttaaatagt acgtaacacc taaaagacta
     8161 aagaaagttt ttttattttc aaatccttca ttgcgtaact gttcatagga aaatcctttc
     8221 gtgaaatcca ttgaaacaaa atgaaggtta tttggaattt caagctctgc ttcttttact
     8281 cttatcttct taaactgttg cgtagaagga tgatcaactt caaatatttc tattttattt
     8341 tctaattctc ggtgtctaaa actgaacgtg tctaagcctg caccgagtat gacgtattgt
     8401 tttgcgccta gtgtaatttc atgtagtaat actctttcac aatatgccgc acgtgctaaa
     8461 ggtgttggtg ataactgaac ttgcgtaatc cattttaata tttcttttgg attatcttga
     8521 aactgctctg caatctcttt attaaaaaag tgtattcctt gaaccatatt catttcaata
     8581 ttgttacgtt ctttttgtaa aattaaatct ttagctatat aatcatcgaa aattttaggg
     8641 ctttcaaatt cactatgata cgccctaccg aaagctgata ctaacgacgt tacacttgct
     8701 tcaccttgtt tcacgcacat acactccttc attttcacaa caaaaatagg gttcccctgg
     8761 ccaggagaac cctattatac accttatttt tacattggta aattatagca taaataagtt
     8821 tttttgtaaa gttttcagcc tgctaacact ccgacacttt gtatattctc cggcaggaac
     8881 atcgaaatta tatacacaat tttccgaatt atttacccat tcttttttga gcagtattag
     8941 tatcttgaga tactggtata tgttcggtag ctctagataa acctgttttt ggcatattaa
     9001 catagattgc ttcagaagca cctttttcta tttgataggt ttcgtgaaaa acaccgacac
     9061 tcccattatc tttaatctta ttattgtatg atttccaagc atatgaatga ttttttccgt
     9121 gagcatagtt tactaattcg tcgaatccct tccagtattg aattaagatt acttttctcc
     9181 aactaaataa gaattctgta tgtaagaaac cccattgtgg attttgatat aattctttaa
     9241 tcataggtcc catagcatta aaaactggta tccacttcca aaattttaat aggttattaa
     9301 tacgcatacc aataataaaa acaacaaatt cttgatcctt gctattggct atatgcctgc
     9361 cgttaaaaat tttagccccc actttaatcc ccctttttta ctgaacataa ataatgtgca
     9421 gctcccaaat aaaccatatc attttcccat taattttcca attactttaa catagattat
     9481 gaaaggctgt acttaacaat acaaggtata cgtttcccac cttttttatt attaacagct
     9541 attattaata tttgctctaa atctcctttt tcaccaggaa cattttcata atatatttca
     9601 ataacgatag cattgccgcc cctttctaaa cagaatattt tttagttcat ttgagttagg
     9661 acaacatcac actccttcgt attttttgca agactagctt tcaaccttga gggaacaccc
     9721 aaaagctaat caactcccct actctatgaa ataaataagc gaatttaaaa attacataat
     9781 catagattat tatgcaaata cttcctctag gcgccgaaca tggtatataa taataaaata
     9841 atctacttct atatgctcaa ttttacacaa aacagctcat gtatatgaaa gaaaagaggt
     9901 acaaaaatat gaaaacaaaa caaacaattg ctgcgtctac actagctcta gcgatgattg
     9961 ctggcgcaaa ctctactcat gcggaagtaa ctgatgctac tccccagcaa agtacgggag
    10021 atcgattagc tgaaattaag caacataaac aagagttaga tgcgaaattg cagcagcaca
    10081 aagaaaatgt ggatcaaaca ttaaatgaac ttaaccaagt taaggaaaat gttgatacaa
    10141 aggtgaatga actacatgaa cgtaaacaag ttgcagatga aaaaattggc gagattaaac
    10201 aacataaaca agagttagat gcaaaacttc agcaagacaa acaaatcgcc gaagataaaa
    10261 tcgctgaaat taaagagcat aaaaaacaag tagaagataa agttgctgaa at
//
