LOCUS       NZ_CP093291            10276 bp    DNA     circular CON 24-MAR-2024
DEFINITION  Bacillus mycoides strain DSM 2048 chromosome, complete genome.
ACCESSION   NZ_CP093291
VERSION     NZ_CP093291.1
KEYWORDS    RefSeq.
SOURCE      Bacillus mycoides (Bacillus weihenstephanensis)
  ORGANISM  Bacillus mycoides
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 5285600)
  AUTHORS   Chiba,A., Peine,M., Kublik,S., Baum,C., Schloter,M. and Schulz,S.
  TITLE     Complete genome sequence of Bacillus mycoides, isolated from an
            agricultural field in Rostock, Germany
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5285600)
  AUTHORS   Chiba,A., Peine,M., Kublik,S., Baum,C., Schloter,M. and Schulz,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2022) Research Unit Comparative Microbiome
            Analysis, Helmholtz Zentrum Muenchen, Ingolstaedter Landstr.1,
            Neuherberg 85764, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 15-MAR-2021
            Assembly Method                   :: HGAP v. 4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 1166.0x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022630575.1-RS_2024_03_24
            Annotation Date                   :: 03/24/2024 09:23:57
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,704
            CDSs (total)                      :: 5,549
            Genes (coding)                    :: 5,305
            CDSs (with protein)               :: 5,305
            Genes (RNA)                       :: 155
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 108
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 244
            CDSs (without protein)            :: 244
            Pseudo Genes (ambiguous residues) :: 0 of 244
            Pseudo Genes (frameshifted)       :: 93 of 244
            Pseudo Genes (incomplete)         :: 175 of 244
            Pseudo Genes (internal stop)      :: 63 of 244
            Pseudo Genes (multiple problems)  :: 79 of 244
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 07:31:00
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4368602
            Orig. end                         :: 4378878
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP093291.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Research Unit Comparative
            Microbiome Analysis, German Research Center for Environmental
            Health, Ingolstaedter Landstr. 1 85764 Neuherberg.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10276
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="10"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10276
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10276
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5276](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5276)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(309..1439)
                     /gene="dnaN"
                     /locus_tag="MN093_RS22645"
                     /old_locus_tag="MN093_22645"
     CDS             complement(309..1439)
                     /EC_number="2.7.7.7"
                     /GO_component="GO:0009360 - DNA polymerase III complex
                     [Evidence IEA]"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /gene="dnaN"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394467.1"
                     /locus_tag="MN093_RS22645"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MN093_22645"
                     /product="DNA polymerase III subunit beta"
                     /protein_id="WP_131246264.1"
                     /transl_table=11
                     /translation="MEFIVNHKQFTQALSEVSKAISTKTLIPILSGIKITADQSGITLI
                     ASNSNIFIEKFIPISIEGVQITTILKAGSIVVPAKYFIEIIKKMPSDILIKSMNEQLIT
                     IQSDEITLNLNGFSANDFPNVPFIDEHAEIQVEMEQLIEVFKQTVFATAKNESRPVLTG
                     VHFVIDHNKLICAATDSHRLALREIPISSHAKLNCIVPSSTISELLKLMNSNSNLVYIY
                     LSESHIIFKFGTITLYSRLIEGKYPNISGLIPNESQTVINIDRKKILQGVDRSSLLASE
                     RANNNVNLEIIDECTIQISSNASQIGKISEKQQIDAIHGEKQLNISFDGRYMVDTLKAI
                     KEDRVTLSFGGSMRPILIEAGEQSAAVYLISPVRTY"
     gene            complement(1470..1913)
                     /locus_tag="MN093_RS22650"
                     /old_locus_tag="MN093_22650"
     CDS             complement(1470..1913)
                     /EC_number="3.6.-.-"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757615.1"
                     /locus_tag="MN093_RS22650"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MN093_22650"
                     /product="NUDIX hydrolase"
                     /protein_id="WP_131246265.1"
                     /transl_table=11
                     /translation="MKKVNVTYALLYDETHEKLLMVKNKGENGSYYTLPGGAVKFGETL
                     EEAAIREVKEETGLDISVKGVCSISEAFFEERGHHAIFFNFLGEITGGKICISRPKEIE
                     EITWMELDSAEPYLRIPEHLKSLLQKKETVPYIFNGTIIHQSS"
     gene            complement(2197..3405)
                     /locus_tag="MN093_RS22655"
                     /old_locus_tag="MN093_22655"
     CDS             complement(2197..3405)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1137: Major
                     facilitator superfamily MFS 1"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814376.1"
                     /locus_tag="MN093_RS22655"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MN093_22655"
                     /product="OFA family MFS transporter"
                     /protein_id="WP_241875572.1"
                     /transl_table=11
                     /translation="MKQTSINPLLIVLGTIIVQIGLGTIYTWSLFNQPLVSKFGWNLNS
                     VAITFSITSFSLSFSTLFAGKLQQKFGLRKLIATAGIVLGLGLILSSQVSSLPLLYLLA
                     GVVVGYADGTAYITSLSNLIKWFPNRKGLISGISVSAYGMGSLIFRYINGNLINSLGVS
                     QAFLYWGIIVLLLVLIGSFFLREAIVSNTATETLHNDYTPREMMKTKQVYLLFFMLFTS
                     CMGGLYLIGMVKDIGVQLVGLSAATAANAVAMIAIFNTIGRIILGTLSDKIGRLKIVSA
                     TFIIIGLSVFTLSFIPLNYGIYFACVASVAFCFGGNITIFPAIVGDFFGLKNHSTNYGI
                     VYQGFGFGALAGSFIGALLGGFQPTFITIGVLSIISFVISILIRPPNAEKKKEIKQLNR
                     KIA"
     gene            complement(3684..3827)
                     /locus_tag="MN093_RS22660"
                     /old_locus_tag="MN093_22660"
     CDS             complement(3684..3827)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017657232.1"
                     /locus_tag="MN093_RS22660"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MN093_22660"
                     /product="hypothetical protein"
                     /protein_id="WP_002013225.1"
                     /transl_table=11
                     /translation="MKNEVNKNKKQNIKDVNTEQNAIYSDPKDAANMQTVPQPKDFEEI
                     EY"
     gene            complement(3859..4887)
                     /locus_tag="MN093_RS22665"
                     /old_locus_tag="MN093_22665"
     CDS             complement(3859..4887)
                     /EC_number="3.1.1.103"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1053: beta-lactamase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002085304.1"
                     /locus_tag="MN093_RS22665"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MN093_22665"
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="WP_241875573.1"
                     /transl_table=11
                     /translation="MYKYEKLISWVENIKETNQSSTAALCIIKDNKIVLEHYSGTHSNT
                     PTSKKVTISSQFNVASARKSYLGLMVAYAVYEGKIKSLDDEAVNYFKEYDPSLLGKTTI
                     RHLVTHSHGLNETDDGTAFREFEPGQAWAYRDINVRMVTQLIYQLYNKSFPELLKERVF
                     IPADFKKTGWRIKQDDNLVKVVVNPDEDAISSVGAVDDGSEKNLFVSAREFAFWGNLHL
                     NKGFLNGKQIVPKEVIALATSLQSPEYTNKELPQNGLFWFVQNEPALFSELGERVPKGS
                     YQILGITGPTILVIPEYNVVVAKMYNKRYNYGGDNYLYYLREFSNLVADTFSSSKNTMV
                     RV"
     gene            complement(5001..5276)
                     /locus_tag="MN093_RS22670"
                     /old_locus_tag="MN093_22670"
     CDS             complement(5001..5276)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03601"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001869.1"
                     /locus_tag="MN093_RS22670"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MN093_22670"
                     /product="heterocycloanthracin/sonorensin family
                     bacteriocin"
                     /protein_id="WP_001001869.1"
                     /sec_met_domain="TIGR03601 (E-value: 7.5e-39, bitscore:
                     130.3, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNEFQQELQALNLNAYQPGNVVYWDQQQNQYPYYYIQDDARRCGG
                     CGGGGRCGGCGGRCGGCGGGRCGGCVGCVGCVGCFSCSNCWNWWFI"
     misc_feature    5290..5305
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 26.71"
                     /tool="antismash"
     gene            complement(5476..5922)
                     /locus_tag="MN093_RS22675"
                     /old_locus_tag="MN093_22675"
     CDS             complement(5476..5922)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836819.1"
                     /locus_tag="MN093_RS22675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MN093_22675"
                     /product="NUDIX domain-containing protein"
                     /protein_id="WP_241875574.1"
                     /transl_table=11
                     /translation="MKSKFHHIVRAIMIKDEKLLVAEYIGHHYFLPGGHVEIGESAENA
                     LIRELREELGVTCSIKQFLGVIEKQWQAKETLHHEINHIFEIDSNELKSDFTPISKESH
                     LAFHWIDFNKENINSYTIMPTPSVKELLERKLSDELLTCWISNF"
     gene            complement(6295..6864)
                     /locus_tag="MN093_RS22680"
                     /old_locus_tag="MN093_22680"
     CDS             complement(6295..6864)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002065694.1"
                     /locus_tag="MN093_RS22680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MN093_22680"
                     /product="DUF4256 domain-containing protein"
                     /protein_id="WP_002013217.1"
                     /transl_table=11
                     /translation="MKESNKKELSLEQREELLQTLKDRFENNMNRHKGLEWANVQERLD
                     ANTEKLWSLNEMERTGGEPDVVDFDKEKGEYIFYDCSAESPKGRRSVCYDLEALESRKK
                     HKPENNAIDMATAMGIELLTEEEYRTLHNLENFDMKTSSWVQTPSDIRELGGALFCDYR
                     FGHVFVYHNGAESYYAARGFRGSLRV"
     gene            complement(7048..7512)
                     /locus_tag="MN093_RS22685"
                     /old_locus_tag="MN093_22685"
     CDS             complement(7048..7512)
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002136577.1"
                     /locus_tag="MN093_RS22685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MN093_22685"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_241875575.1"
                     /transl_table=11
                     /translation="MNVQLKIVTRENWEDALKLQVKENQINFVPAVAVSLAKVYIKPDG
                     ENVEYIPFAIYDGDLIVGFIMHAFVRESTDMYWINGFIIDEKEQGKGYGKAALQEIINL
                     IKNNFKECEEIRLTVHKDNISAKKLYECYDFKPLGNVYDGEEVYRLLLLK"
     gene            complement(7590..8513)
                     /locus_tag="MN093_RS22690"
                     /old_locus_tag="MN093_22690"
     CDS             complement(7590..8513)
                     /EC_number="3.2.2.-"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016087665.1"
                     /locus_tag="MN093_RS22690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MN093_22690"
                     /product="nucleoside hydrolase"
                     /protein_id="WP_241875576.1"
                     /transl_table=11
                     /translation="MEKVLFFGDPGIDDSFAIMYGLLHPEIEIVGIVTGYGNVEHIHAA
                     HNAAYILQLANRQDIPVISGATGPLTNEITTYYPEIHGPEGLGPIHVPESVLSIPIYNF
                     GSIAAILVKYGEDLTIVDVGRSTSLAIAFNLWNDLMLNIKGIYLMGGVFLEAGNVTPLA
                     EANVYGDPIASQIVFQQAKNITLFPLNVTNKTVLTPSVFTCILVNTQNPFKSIMKPLYD
                     YYLSAYQKLNPSIEGPLLHDVLAISGLVNPTFLNYTSRKVTVDICGETKGQTFADFRPH
                     SKSEGARIALQLDEEKFIQDFMKIML"
     misc_feature    8762..8778
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 22.1"
                     /tool="antismash"
     gene            complement(8796..9707)
                     /locus_tag="MN093_RS22695"
                     /old_locus_tag="MN093_22695"
     CDS             complement(8796..9707)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1166: transporter,
                     EamA family"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002136580.1"
                     /locus_tag="MN093_RS22695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MN093_22695"
                     /product="DMT family transporter"
                     /protein_id="WP_241875577.1"
                     /transl_table=11
                     /translation="MHNKKWDFRIVCAHAFTILIWGTAFPGIRMGLEASPPEHLTLLRL
                     LIASLILFLFSLIYKLRLPDLKDIPVIFIFGALGFTIYHIALNYGEKSVNAGPASLIVS
                     VTPILTAILASVFLNEKMKLNGWIGGVISFVGIAFLSLSQGDSIQLNSGGLLILLAAIS
                     ESLFFVFQRPYLKKYGFLPFTIYTIWSGTVFMLIFLPGIYEEIVAAPIEVTLSVIYLGL
                     FPTVLPYIALAYITSHASASEATSSLYLTPVTACFIAWIWLGEVPTFVSIIGGGITILG
                     VVIAHLTFNKDDYRQIESEKVW"
ORIGIN
        1 gagtgtgata tgtaaattca ttaagtagct ctactaaata gacatacatg ccaccagaat
       61 atgaggaatc ctcccattta ataatgcctt taaattgaat ttgattttgt tcaacatcta
      121 tatttgtttc ttcttttatt tcacgaattg cattttctaa cggtgtttct cctttttcta
      181 tcttccctcc tacaccgttc cataatcctt ttacaggatc atattctcta tttaacataa
      241 gtatttcttc atttcttttt ataaaacata aagtgtgctt gtacataatt taattccttc
      301 ctttgttctt aataagttct aacaggcgat attaaatata ctgctgcaga ttgttcacct
      361 gcttcaatta atatcggtct catggaaccg ccaaaactta aagtaactct atcttctttt
      421 attgctttta aagtatcgac catatatcgt ccgtcgaaag atatatttaa ttgcttttca
      481 ccgtgaatcg catctatttg ttgtttctcg gatattttac caatctgaga agcattagaa
      541 gagatttgta ttgtgcattc gtcgatgatt tctaagttaa cgttgttgtt tgcccgttca
      601 ctagccaata aacttgaccg atctacacct tgtaaaatct tttttctatc tatattaata
      661 actgtttggg attcatttgg aattaggccg gatatattag gatattttcc ttcaataagt
      721 cttgaataca acgtaattgt accgaattta aaaataatgt gactctctga aagatatatg
      781 tatactaaat ttgaattact gttcattaat tttagtagtt cactaatagt ggaacttggt
      841 acaatgcaat ttaatttcgc atgtgaagaa atgggtattt cacgtaaagc cagtctgtgc
      901 gaatcagtag cagcacaaat taatttgtta tgatcaataa cgaaatgtac gccagtaaga
      961 actggtctag attcattttt agcagttgca aagacagttt gtttgaatac ttcaatcaac
     1021 tgttccattt ctacttgtat ttctgcatgt tcgtctataa atggtacatt tggaaagtcg
     1081 ttcgccgaga atccatttaa gtttaaagta atttcatcag attgaattgt aatcaactgc
     1141 tcattcatgc tttttattaa aatatcactt ggcattttct taataatttc aatgaaatac
     1201 tttgctggta caacaatact tcctgctttt aaaatagttg taatttgtac accttcaatt
     1261 gaaataggta taaatttttc aatgaaaata ttcgaattac ttgcgattaa agtaattcca
     1321 gattgatctg ctgttatttt tattccggat aatataggaa ttaaagtttt tgttgagata
     1381 gctttactta cttctgaaag agcttgtgta aattgtttgt gattaacgat aaactccatt
     1441 tttcccccgc cttaaatgaa atactatctt tatgaagatt gatgaataat agttccatta
     1501 aaaatataag gaactgtttc tttcttttgt aaaaggcttt ttaagtgttc tggtatacgt
     1561 aaataaggtt ctgctgaatc taattccatc caggtaatct cctcaatctc ttttggccgt
     1621 gatatacaga ttttccctcc ggttatttct cctaaaaaat taaagaagat tgcatgatgt
     1681 cctctctcct caaaaaaggc ttcactaatg gaacaaaccc cttttacaga tatgtctagc
     1741 cctgtttctt ctttcacttc acgaattgct gcctcttcta acgtttcccc gaatttaact
     1801 gctccacctg gtaaagtgta ataagaacca ttttcccctt tgtttttaac cattaatagc
     1861 ttttcatgag tttcatcata tagaagtgcg tatgtaacat ttactttttt catatgtaac
     1921 ccccttgaat ggtaaaaatt ttctgtcttt atttcattct atcattaaat tgtttggatt
     1981 cataaaaagt aatttgagta aataactaat acgaaacgat atcgtgttca tagacacaca
     2041 aaagaaaaga gcatattttg aaaaagatta atcataatac actctttaat gattcattaa
     2101 gtacagggaa aaaattagat tttttgaatg tcatccattg tttatacatt tttttgtaaa
     2161 gcaaaagagc cctagtataa ggactctttc ttagcattaa gctatttttc gatttaactg
     2221 ttttatttcc ttcttctttt ctgcatttgg agggcgaatt aatatagaga taacgaatga
     2281 tataatactt aaaacaccga ttgtaatgaa ggttggttga aaccctccaa gtagtgctcc
     2341 aatgaatgat cctgcaagtg caccgaatcc gaaaccttgg taaacaatcc cgtagtttgt
     2401 actatggttt tttaatccga agaaatcccc gacaatggct gggaatatgg tgatattacc
     2461 accaaagcaa aaggcgacac ttgctacaca agcaaaataa atcccgtaat ttagtggaat
     2521 aaaacttaaa gtgaagacgg acaatccaat aataataaat gtagcagaga cgatttttaa
     2581 tcgacctatt ttatctgata acgttccaag aataattcta cctattgtat taaagattgc
     2641 gatcattgcg acggcattag cggcagtcgc tgcactaaga ccaacgagct gcaccccgat
     2701 gtcttttacc ataccgatta aatacagtcc gcccatacat gatgtgaata acatgaaaaa
     2761 taagagatat acttgttttg ttttcatcat ttcacgcggc gtataatcat tgtgtaatgt
     2821 ttcagttgca gtgttactta caattgcttc acgtaagaag aatgatccta tcaacactaa
     2881 tagtaagacg ataatacccc aatataaaaa tgcttgtgat actccaagac tgttgataag
     2941 atttccgttt atatatctaa agattaagct tcccattcca tatgccgaaa cagatatacc
     3001 agaaataagt cctttacggt ttggaaacca tttaattaaa ttagatagtg atgtaatata
     3061 tgctgttcca tcagcgtatc caacgacaac accggctaat aaataaagta atggtaatga
     3121 ggaaacttgt gaactaagta ttaaaccgag tcctaaaaca attcctgcag tagcgataag
     3181 tttacgaagt ccaaattttt gttgtagctt tcctgcaaat agagttgaga atgataaaga
     3241 aaaactcgta attgaaaacg tgatcgcgac tgaattgagg ttccatccaa acttacttac
     3301 aaggggttga ttaaataaac tccatgtgta aattgttcct aggccaattt gaacaatgat
     3361 tgtacctaga acaattagca atggatttat agatgtttgt ttcatgctaa ctgtcccctt
     3421 cttttatcat gacatgaatt ttcacagtta gtatatccat gataaattgc aagatactgt
     3481 acggaaaatg gaaattgagt tagcttacac aaaaagacac ttcatcaaga agtgcctttt
     3541 tgtaggttat atacagcatt taaagaaagc tttgttaaga taagacattt tcgaatgctt
     3601 atgtatatat gttatcaaat aatttgctga aagtgatgag acttaagacg tatttttctt
     3661 tcataaataa gtcggagaga aaattaatat tcaatttcct caaagtcttt cggctgcggt
     3721 acagtttgca tattagctgc atctttcgga tcgctataaa tcgcgttttg ttctgtgttt
     3781 acatccttta tattttgctt tttattttta ttcacttcat ttttcattaa agttcactcc
     3841 tttttattat aggagtagtt ataccctaac catagtattt ttactgctac taaatgtatc
     3901 agcgactaaa ttgctaaatt cacgtaaata atataaataa ttatccccac catagttgta
     3961 tcttttattg tacattttag caacgacgac attatattct ggaatgacta atatcgttgg
     4021 tcctgtaatt cctaatattt gataggaacc tttaggaact cgctcaccaa gttcgctaaa
     4081 taaagcaggt tcattttgaa cgaaccaaaa caacccgttt tgaggtagtt ctttatttgt
     4141 atactctgga ctttgtaagc tcgtagcaag tgctataact tcttttggca caatttgttt
     4201 accatttaaa aaccctttat ttaaatgaag gttaccccaa aatgcaaatt ctctcgcaga
     4261 gacaaaaaga tttttctcgg agccgtcatc tactgcacca acactactta ttgcatcttc
     4321 atctgggttt acaacaacct taactaaatt atcatcttgt tttattctcc atccagtttt
     4381 tttgaaatca gcaggtatga atacacgctc ctttaatagt tcaggaaaac tcttattata
     4441 cagttgataa ataagctgcg tcaccattct tacattaata tctctgtacg cccaagcttg
     4501 tcccggctca aattcacgaa aagctgtccc atcatcggtt tcatttaaac cgtgtgaatg
     4561 agttactaaa tgtcttatcg ttgtctttcc aagtaatgaa ggatcatatt ctttaaaata
     4621 atttacagcc tcgtcatcaa gagattttat tttcccctca taaacagcat aagctaccat
     4681 taatcctaaa tagctttttc ttgcggaagc gacattgaac tgtgaagata tggttacttt
     4741 tttactagta ggagtatttg aatgcgttcc actataatgc tctagcacaa tcttattatc
     4801 tttaataata caaagtgctg ccgtagaact ttgatttgtc tctttaatgt tttctaccca
     4861 tgaaattaat ttttcatatt tgtacaaata aatatccccc taaaacatat aaaaataaca
     4921 gcatgaaaga aaagtttcaa tacattataa ctttgcatct gcacttttct ttcatgtgaa
     4981 atattataga tctataatac ttaaataaac caccagttcc agcaattaga acagctgaaa
     5041 caaccaacac agcctacaca gcctacacaa ccgccgcaac gtccgccgcc acaaccacca
     5101 cagcgaccac cacaaccgcc acaacgtccg ccaccaccgc atccgccaca acgacgtgca
     5161 tcgtcttgaa tgtagtaata tggatattga ttttgttgct gatcccaata cacaacatta
     5221 ccaggttgat aagcattaag gtttaacgct tgtagttctt gttgaaattc attcattatg
     5281 aaaccctcca tttacaaatg taaattccgt ccaaattttc gagtttattt acttttaaaa
     5341 ataaagtaga aataaggcta tagactaaat gcgtacatca acaaaatatg actttacact
     5401 aggtgatgta ccttgtttat gagcctattt tctttatatt cttttagtga acggaattaa
     5461 tataaataga gacaattaaa agttgctaat ccagcaagtt aatagttcat cacttaattt
     5521 tctttctagt aattctttta ctgaaggtgt cggcattatt gtataggaat ttatattttc
     5581 tttattaaaa tcaatccagt gaaatgctaa atgggactct ttagatattg gagtgaaatc
     5641 ggattttaac tcatttgaat ctatttcaaa aatgtggtta atttcatggt ggagagtttc
     5701 tttggcttgc cattgttttt ctatgacgcc taaaaattgt tttatactgc aagttactcc
     5761 gagttcttct cgtaactctc ttattagtgc attctctgct gattccccaa tttcaacatg
     5821 gccaccgggt aaaaaatagt gatgtcctat gtattcagca actagtaatt tttcatcttt
     5881 tatcatgata gctcgtacaa tatgatggaa tttacttttc attttgtatt cctcctaatc
     5941 attcacttta tacttgctcg gaaattttca tttaaaagcg ggataatata aatcataggt
     6001 gtttcatatg ggtgaatttc attgataatt gcaagagtac cttttacaag ttctcgttta
     6061 tacgttaatt aaaactaata attttgataa atgattctaa aaaagagtat gtttatgaag
     6121 aaaattatca aaacatactc ttaatatttt tagttgattc attcttttac aataaggttt
     6181 gattagccct attctttgcg gcctcaaaat cgaagccgta gctaaagtat gcaatcacta
     6241 ctattctcta agcaaatcta ttctctactt caaatttacc tgcctgtaca aattttagac
     6301 tcttaacgaa ccacgaaagc ctcttgcggc atagtaagat tctgccccat tgtgatacac
     6361 aaagacgtgt ccaaagcgat aatcgcaaaa aagggcaccg ccaagttctc taatatcaga
     6421 aggtgtttgc acccaactcg acgtcttcat atcgaaattt tcaagattat gtaatgtacg
     6481 atattcttct tccgttaata gttcaatacc catggcagtt gccatatcaa tagcgttatt
     6541 ttctggttta tgtttctttc ttgattctag tgcttcaaga tcgtaacaaa cacttctacg
     6601 acctttagga ctttctgctg aacaatcata aaaaatatat tcgccttttt ctttatcaaa
     6661 atcaacaaca tcaggttcgc caccagttcg ttccatttcg ttgagcgacc acaacttttc
     6721 agtattagca tccagccttt cttgaacatt agcccattct aaacctttat gacggttcat
     6781 attattctca aaacgatctt tcaatgtttg tagtaattct tcacgttgct ctagtgacaa
     6841 ctctttttta ttgctttctt tcatattagt tcctccattg atgtttttat taatgatagt
     6901 ttaagtatat ataaatactg ttttataata aaatgtttaa ataattgcgt gaattgagtt
     6961 tgcgataatt tatatgaatt agtacaacca attctccgca atatgaccct ttaacggaac
     7021 aacttattgt ttcaaaacag tacatcttta tttcaacaac aataagcgat atacttcctc
     7081 accgtcatat acatttccta atggtttaaa atcataacat tcatatagtt tctttgcaga
     7141 aatattatct ttatggacag ttaatctaat ttcctcacac tctttaaagt tattttttat
     7201 taagttaatg atttcctgta atgctgcttt tccatatcct tttccttgct ctttttcatc
     7261 aatgataaat ccgttaatcc aatacatatc tgttgactct cgtacaaaag catgcataat
     7321 aaaaccaact ataaggtcac catcatatat agcgaatgga atatattcga cattttcacc
     7381 atctggtttt atgtatactt tagcaagtga tactgccact gccggaacaa agtttatttg
     7441 gttttcttta acttgtaact ttaatgcgtc ttcccaattt tctctcgtaa caatttttaa
     7501 ttggacgttc actattttaa gctcctttaa aaagagaatt tatcttttat attcaacgga
     7561 gtgaaatgat tccctttttc gacatcaact tataacatga ttttcataaa atcctgaata
     7621 aatttttcct catctaattg taacgctata cgcgcccctt cagatttaga gtgtgggcga
     7681 aaatcagcaa aagtttgtcc cttcgtttcg ccacatatgt ctacagtcac ttttcgagat
     7741 gtataattta aaaatgtagg attcactaaa ccgcttatag caagtacatc gtgtaataat
     7801 ggcccttcaa tagatggatt gagtttttgg tatgctgata aataataatc gtacaggggt
     7861 ttcataatcg acttaaatgg attttgtgta ttaactaaaa tgcaagtaaa tacacttggg
     7921 gttaaaaccg ttttatttgt tacatttaaa gggaataacg ttatattttt cgcttgctga
     7981 aatacaattt gacttgctat aggatctccg tacacgtttg cctctgctaa tggagtaaca
     8041 ttccctgctt ctaaaaagac accacccata agataaattc cttttatatt taacatcaag
     8101 tcgttccata aattaaaagc aatcgctaac gaggtagatc ttcctacatc gacaattgtt
     8161 aaatcttctc catactttac aagaatcgca gcaatactac caaaattgta tataggaata
     8221 gataacacac tttccggtac atggatgggc cctaatcctt ctggtccatg aatttcaggg
     8281 taatacgttg taatttcatt tgtaagtggg cctgttgcac cgctaattac aggaatatct
     8341 tgtctgtttg ctaactgtaa aatatatgcg gcattatgag cggcatgtat atgttcaaca
     8401 tttccatatc cagttacgat accaacgatt tcaatttcag gatgcagtaa gccatacata
     8461 attgcaaacg aatcatcaat acctgggtct ccaaaaaata aaaccttctc caagaaatgc
     8521 actcctttca taggtaagat aattatgtat atgtggaaat aatttaaatc tatacagttc
     8581 taagcaaata ttaatggtga gtaatgtatt gataaaaaag aactagcatt atgctagttc
     8641 ttttgcagat aaataatcta ccagtttaat taaatctcgt acaaatattg gactagctga
     8701 ttttaatata gattttttca ctatatttat agaattgaag gtcttttatt aatccgaaat
     8761 tttgcattta aattgcaaat actcgcaggg atgtcttacc atactttttc actctcaatt
     8821 tgcctataat catctttatt aaatgttaaa tgagcaatca caactccaag tatagtaatt
     8881 ccccctccaa ttatcgaaac aaaggttggc acctcaccta accatatcca agcgataaaa
     8941 catgcagtta ctggtgttaa atatagagaa cttgttgcct cagaagcact ggcatgggat
     9001 gtaatatatg ctaatgcaat atacggcagt actgtcggga acaaacctaa atatataaca
     9061 ctcaaagtaa cttctatagg agctgctaca atttcttcat aaatccctgg caaaaaaata
     9121 agcataaata cagtaccaga ccaaattgta tatattgtaa atggcaagaa accgtacttt
     9181 tttaagtatg gcctttgaaa aacaaaaaat agactttctg aaattgccgc caataatatt
     9241 agtaatcccc cactattcaa ttgaatggaa tctccttgac ttaacgataa aaaagcaatt
     9301 cctacaaaac taattacacc accgatccaa ccatttaatt tcattttttc attcaaaaaa
     9361 acagaagcaa gaattgccgt taatataggt gttactgata caattaaact agcaggtcca
     9421 gcattcacac tcttttcacc gtagttcagt gcaatatgat aaattgtaaa tccaagagca
     9481 ccaaatataa aaattactgg aatatctttc aaatcaggta aacgtaattt gtatataagt
     9541 gaaaacagaa aaagaatcaa agaagcgatt aataatcgta gcaaagtaag gtgttcaggg
     9601 ggggaagctt caagccccat ccgaattcct ggaaaagctg ttccccatat aagaatcgta
     9661 aaagcgtgag cacatacaat acgaaaatcc cattttttat tatgcatatt ttctctccct
     9721 ctccaataaa ccagccattt tctaattacc aattatttct tttcatcata tttaatagtt
     9781 gatacaatgt cttcaaccag ttaatcctcg aaaaaaccaa ccactttgca tttttaaaat
     9841 gaatgaggga gtttttaata tgtctactga taaaaaactg cctaaatatc ggcagataat
     9901 agactatatg aaagaaaaaa ttgaatacgg agaatggccg attggaagta aggttcctag
     9961 ccagcggcag ttagcgaaat tatttcatgt aaatcgtagt actgtaataa ctgcactaga
    10021 agaacttatg gcagatggct taattcaagg gcaaatcggg tcaggaacaa ttgtaacgaa
    10081 taatacatgg tcattattag ccaccaatcc cccgcccgat tggagtgaat atgtaaaggc
    10141 tggtattcat aaaccaagca agttaatggt acgagaaata aatgaagcag aagcaaataa
    10201 aaaattgatt catcttagta agggagaact tgcacctcat cttttcccgt tagaaactat
    10261 gcaattcatt atgcgt
//
