LOCUS       NZ_CP093291            10291 bp    DNA     circular CON 24-MAR-2024
DEFINITION  Bacillus mycoides strain DSM 2048 chromosome, complete genome.
ACCESSION   NZ_CP093291
VERSION     NZ_CP093291.1
KEYWORDS    RefSeq.
SOURCE      Bacillus mycoides (Bacillus weihenstephanensis)
  ORGANISM  Bacillus mycoides
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 5285600)
  AUTHORS   Chiba,A., Peine,M., Kublik,S., Baum,C., Schloter,M. and Schulz,S.
  TITLE     Complete genome sequence of Bacillus mycoides, isolated from an
            agricultural field in Rostock, Germany
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5285600)
  AUTHORS   Chiba,A., Peine,M., Kublik,S., Baum,C., Schloter,M. and Schulz,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2022) Research Unit Comparative Microbiome
            Analysis, Helmholtz Zentrum Muenchen, Ingolstaedter Landstr.1,
            Neuherberg 85764, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 15-MAR-2021
            Assembly Method                   :: HGAP v. 4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 1166.0x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022630575.1-RS_2024_03_24
            Annotation Date                   :: 03/24/2024 09:23:57
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,704
            CDSs (total)                      :: 5,549
            Genes (coding)                    :: 5,305
            CDSs (with protein)               :: 5,305
            Genes (RNA)                       :: 155
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 108
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 244
            CDSs (without protein)            :: 244
            Pseudo Genes (ambiguous residues) :: 0 of 244
            Pseudo Genes (frameshifted)       :: 93 of 244
            Pseudo Genes (incomplete)         :: 175 of 244
            Pseudo Genes (internal stop)      :: 63 of 244
            Pseudo Genes (multiple problems)  :: 79 of 244
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 07:31:00
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4135638
            Orig. end                         :: 4145929
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP093291.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Research Unit Comparative
            Microbiome Analysis, German Research Center for Environmental
            Health, Ingolstaedter Landstr. 1 85764 Neuherberg.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10291
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="7"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10291
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10291
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5291](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5291
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            158..1201
                     /locus_tag="MN093_RS21440"
                     /old_locus_tag="MN093_21440"
     CDS             158..1201
                     /EC_number="1.2.1.11"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1219: N-acetyl-gamma-glutamyl-phosphate reductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017561445.1"
                     /locus_tag="MN093_RS21440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MN093_21440"
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="WP_131247169.1"
                     /transl_table=11
                     /translation="MSEKSYHVAVVGATGAVGQKIIALLERVTKFNIEEITLLSSKRSA
                     GKIVQFKGREIKIQEAKSTSFEGVDIVFFSAGGEVSRQYVNHAVSSGAIVIDNTSEYRM
                     EHDVPLVVPEVNAHTLREHKGIIAVPNCSALQMVTALQPIRKSFGLERIIVSTYQAVSG
                     SGIHAIHELKEQAKSMLTGEEVKSTILPAKKDKKHYPIAFNVLPQVDIFTDNDFTFEEV
                     KMIQETKKILEDPNLKMAATCVRVPVVSGHSESVYIELEKEATVAEIKEALLNAPGVIL
                     QDNPSEQIYPTPLYAEGKRDTFVGRIRKDPDTPKGFHLWIVSDNLLKGAAWNSVQIAET
                     LVEKGII"
     gene            complement(1309..1728)
                     /locus_tag="MN093_RS21445"
                     /old_locus_tag="MN093_21445"
     CDS             complement(1309..1728)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017674076.1"
                     /locus_tag="MN093_RS21445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MN093_21445"
                     /product="hypothetical protein"
                     /protein_id="WP_241875545.1"
                     /transl_table=11
                     /translation="MNKSKPVDELTIEDLKQNPIWEWAIDEECDETWVKPVETINFTEE
                     LNGSIVLGELIIHNDEKSPMMCSIDIENNEVLISSIVFYNEKENEYFAIEDVVKKVEMP
                     ISINIYLTINGEPRFLKFSANKIDIYKNTIKTNLN"
     misc_feature    complement(1938..1951)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: medium, score: 21.79"
                     /tool="antismash"
     gene            2120..3244
                     /locus_tag="MN093_RS21450"
                     /old_locus_tag="MN093_21450"
     CDS             2120..3244
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016092615.1"
                     /locus_tag="MN093_RS21450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MN093_21450"
                     /product="C45 family peptidase"
                     /protein_id="WP_241875546.1"
                     /transl_table=11
                     /translation="MYQIKGYFSSLKGNHYEIGKQQGEFVKKHPYLIPQFIQQENLIWD
                     NHWTESRNVLKQYCPGINEEIEGFCEVLKIPSRNMMYYYQTLLKAGCSHCAVLPKKTDL
                     KHTYVLRNFDLSPKIDDMRFCSTHVEGAYAHSGFSTQYFGRTEGVNEHGLSITFSACGQ
                     PVGNIEGLRKPMVNGLQCFAVIRVILEKCRNTHEATSLIKEMPIASNINIIVADPLNAA
                     HIEIFDGHKSITTINEESQAFTVSTNHAVSSPIQKLTNRKLEQSTKRYHILHEHLNRNE
                     QVSMESLKGLVEEEYPIGVTVHNYEEWFGTLHSVLIDLQDRTLNICFGSPLLNEWYSLK
                     VGESLPFSEVNVNFKNKTYADFWREEKNEMTPKD"
     gene            3298..3561
                     /locus_tag="MN093_RS21455"
                     /old_locus_tag="MN093_21455"
     CDS             3298..3561
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002085637.1"
                     /locus_tag="MN093_RS21455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MN093_21455"
                     /product="hypothetical protein"
                     /protein_id="WP_131247165.1"
                     /transl_table=11
                     /translation="MLQAMLPIVLILFLVALMYITYQKRKKAGIKGLKSSLTSICCFLI
                     ATLNLFAYWLHFGGIFTWLVSIVLLLVGAYFTKYIPISKKVH"
     gene            complement(<3674..>3760)
                     /locus_tag="MN093_RS21460"
                     /old_locus_tag="MN093_21460"
                     /pseudo=""
     CDS             complement(<3674..3760)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675870.1"
                     /locus_tag="MN093_RS21460"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /old_locus_tag="MN093_21460"
                     /product="integrase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MISLTLRVYSHVLPNMQEDAAKTFAKSIF"
     misc_feature    3841..3852
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: medium, score: 18.58"
                     /tool="antismash"
     gene            3942..4169
                     /locus_tag="MN093_RS21465"
                     /old_locus_tag="MN093_21465"
     CDS             3942..4169
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="MN093_RS21465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="MN093_21465"
                     /product="hypothetical protein"
                     /protein_id="WP_002085632.1"
                     /transl_table=11
                     /translation="MKGDDKNHEIRFKQIERTLKYALDNDQRQIIELKYFGSEKVKDSY
                     VYNELMMRRDSFYENKKIAIRLIATALGII"
     gene            5001..5291
                     /locus_tag="MN093_RS21470"
                     /old_locus_tag="MN093_21470"
     CDS             5001..5291
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03601"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016120187.1"
                     /locus_tag="MN093_RS21470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MN093_21470"
                     /product="heterocycloanthracin/sonorensin family
                     bacteriocin"
                     /protein_id="WP_131247161.1"
                     /sec_met_domain="TIGR03601 (E-value: 4e-37, bitscore:
                     124.8, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNKFQQELQALSLNDYQSGNIVYWDQQNQYPYYYIIDDARRCGGC
                     GRCGGGGRCGGFRCFGCFGCFSCFGCGGCSNCSNCSNCFDGFNGTTVTFEY"
     gene            complement(5624..6526)
                     /locus_tag="MN093_RS21475"
                     /old_locus_tag="MN093_21475"
     CDS             complement(5624..6526)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1014: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000614455.1"
                     /locus_tag="MN093_RS21475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MN093_21475"
                     /product="LysR substrate-binding domain-containing protein"
                     /protein_id="WP_165497915.1"
                     /transl_table=11
                     /translation="MISIELRQLEYFLAVSKELHFTKAAEKLNISQPSLSQQIRALEHE
                     VGMPLFDRIGKKISLTEAGRVLLSHSKTIFHEVEQARSAIQDLNGLHHGSLTIGSLLTV
                     VNYLLPPAILDFNKQYPNIELSVLGLRTGEIREKLLQNELDIGITFLPVQDKEIVSIPL
                     YKSELTLVVPTSHSLTERKYVSIEELQDYPLILLPKNFYLTELITSHCQNFNFKPKPIL
                     EISTMESLIQMVAQGMGITVLPKPYIDFLQNEHIQAIKIENPTPTIDIGLIYRKDKYMC
                     AATREFIEQLKLTVHSLQN"
     gene            6656..7981
                     /gene="gabT"
                     /locus_tag="MN093_RS21480"
                     /old_locus_tag="MN093_21480"
     CDS             6656..7981
                     /EC_number="2.6.1.19"
                     /GO_function="GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA]"
                     /GO_process="GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (34-374). E-value: 6.7e-122.
                     Score: 398.7. Matches aSDomain:
                     nrpspksdomains_MN093_RS21480_Aminotran_3.1"
                     /codon_start=1
                     /gene="gabT"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002200502.1"
                     /locus_tag="MN093_RS21480"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MN093_21480"
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_241875547.1"
                     /sec_met_domain="Aminotran_3 (E-value: 8.5e-132, bitscore:
                     437.9, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLKSSVLPGSYSQVLQKRYEEAVPTGIYHLTPLYVKEARGAIITD
                     VDGNQFIDFAGGIGMQNVGHCHPKVVRAVQKQAESFIHPCFHVTPYENYITLAEKINEK
                     VPGTSKKKTMFANSGAEAIENAVKIARKVTGRSKVISFERGFHGRTLMTMSLSTKETPY
                     KAGFGPFVSDMHILPYPYYYRAEEGVSPEEVDSQVIYQFEKFLEEVSPEKVAAIVMEPV
                     QGEGGFIVPSISFVKRLREICNQYGILMIMDEVQTGFGRTGKWFATEHFHIEPDIMTLS
                     KSIAGGLPLSAIVGRAELMDIPGRGQIGGTFAGSPLSCAAGLAVFEIMEEEKLVERAQQ
                     IGNRIMSHLHRMQLKYEVIGDVRGIGAMVAMELVTNRITKEPAKELTVKLIEDFWKNGL
                     ISIGAGIFDNVLRFLPPLVISNEEIDRGFEIINESFEKLCRK"
     aSDomain        6758..7777
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MN093_RS21480_Aminotran_3.1"
                     /evalue="6.70E-122"
                     /label="MN093_RS21480_Aminotran_3.1"
                     /locus_tag="MN093_RS21480"
                     /protein_end="374"
                     /protein_start="34"
                     /score="398.7"
                     /tool="antismash"
                     /translation="VKEARGAIITDVDGNQFIDFAGGIGMQNVGHCHPKVVRAVQKQAE
                     SFIHPCFHVTPYENYITLAEKINEKVPGTSKKKTMFANSGAEAIENAVKIARKVTGRSK
                     VISFERGFHGRTLMTMSLSTKETPYKAGFGPFVSDMHILPYPYYYRAEEGVSPEEVDSQ
                     VIYQFEKFLEEVSPEKVAAIVMEPVQGEGGFIVPSISFVKRLREICNQYGILMIMDEVQ
                     TGFGRTGKWFATEHFHIEPDIMTLSKSIAGGLPLSAIVGRAELMDIPGRGQIGGTFAGS
                     PLSCAAGLAVFEIMEEEKLVERAQQIGNRIMSHLHRMQLKYEVIGDVRGIGAMVAMELV
                     "
     gene            7996..8685
                     /locus_tag="MN093_RS21485"
                     /old_locus_tag="MN093_21485"
     CDS             7996..8685
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /GO_function="GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262982.1"
                     /locus_tag="MN093_RS21485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MN093_21485"
                     /product="MOSC domain-containing protein"
                     /protein_id="WP_241875548.1"
                     /transl_table=11
                     /translation="MTKVYEILSLNLGLPKEVMYGGKLIQTGINKKQVKEPVFLSFLKF
                     NGDGQADLVHHGGGDKAVCVYTGDHYPYWEKELNQELVYGAFGENITVSGMREEDVCIG
                     DTFQLGEAIVQVTQPRQPCFKLAKKYNIPKLPLYFQDTGYTGFYFRVLKEGWILPVDTL
                     KLLKPDPKGVSILFANRIMHKEKQNSEGLKRILEVNALSTSWRKTFEKRVKGEEISTKE
                     RLEGTKE"
     gene            8948..9832
                     /locus_tag="MN093_RS21490"
                     /old_locus_tag="MN093_21490"
     CDS             8948..9832
                     /EC_number="2.3.1.-"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526760.1"
                     /locus_tag="MN093_RS21490"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MN093_21490"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_241875549.1"
                     /transl_table=11
                     /translation="MGNVEKMANFLLEEPRREQLFPLFEEVFGITSRTLQDFSEKGYWD
                     YTYKALSFLQDDNAIANVAAFSLPLLINGEKINATGIQSVMTHPNYRRRGLMKQLFSKM
                     IEEIDEQCECAVLFTENPELYKPFGFQIVQEYLMTVSYENKGYKDSQLKKLDFYNEEDR
                     QLIHEIIENGQRLSNKFSTLNYRSSFYLNMYDSTWNENLYYSEKLDALIVFTVENQKLK
                     LFGVFAPVIPILDELCEEISGEFTEIEFYFYPDQLGIDDVQYTELQTSKYLMVRSNKEL
                     DFKGYKFPVLTEF"
     misc_feature    complement(9916..9932)
                     /note="TFBS match to NtrC, Nitrogen regulatory protein C,
                     confidence: medium, score: 22.8"
                     /tool="antismash"
ORIGIN
        1 aaaatgtttt aacatgcctc atttccataa atgatacctc ttttattatc atgatttgtg
       61 aatgattata tcatgtattt caatttttca aatcattaaa tttcttttaa aattaagttg
      121 tggagatatt cattcaattg aaattggagg agaaaaaatg agtgaaaaga gctatcatgt
      181 agctgtagtt ggggctacag gcgcagtagg gcaaaaaatc attgcattgt tagagagagt
      241 aacgaaattt aatatagaag aaattacatt actctcgtca aaacgatctg ctggtaagat
      301 agtacaattt aaaggacgag agattaaaat acaagaggca aaatcaacta gttttgaagg
      361 tgtagacatt gtatttttta gtgctggagg agaggtttct agacaatatg taaatcatgc
      421 agtttctagt ggtgcaatcg taattgacaa cacaagtgaa taccgaatgg aacacgatgt
      481 accactcgtc gttccagaag taaatgcaca tactttaagg gaacataaag ggatcattgc
      541 agttccgaat tgctcagcac tacagatggt aacagctctt cagccaattc gaaaatcatt
      601 tggtttagaa cgaattattg tttcaacgta tcaagccgta tcaggctcag gaattcatgc
      661 gattcatgaa ttaaaagaac aagctaagtc aatgcttaca ggtgaagaag tgaagagtac
      721 tatattacca gcgaaaaaag ataaaaaaca ttatccaatt gcatttaatg ttttgccgca
      781 agtggatata tttacagaca atgatttcac attcgaagaa gtaaagatga ttcaagagac
      841 gaagaaaatt ttagaagatc caaatctgaa aatggcagct acttgcgtca gagttccagt
      901 tgtatcgggg cattctgaat cagtttatat tgaacttgaa aaagaagcga ccgttgcaga
      961 aattaaagaa gcactactga atgcaccagg tgttatttta caagataatc ctagtgaaca
     1021 aatttatcca acgccattat atgcagaggg taaaagagat acatttgtag gtagaatacg
     1081 caaagatccg gatacgccaa aaggattcca tctttggatt gtttctgata atttattaaa
     1141 aggtgcggct tggaactcag ttcaaatcgc agagacattg gtggaaaagg ggattattta
     1201 gtataaattt atataaacat tgaaaatagg aggatatatg ggaaaccgtt aattataatg
     1261 gtttcccatt ttttacttaa tgagtattct cctaacttca atttgaattt aatttaagtt
     1321 agtttttata gtgtttttat aaatatcaat tttatttgca gagaatttta aaaatctagg
     1381 ctctccatta attgtgagat aaatattaat tgagattggc atctcaacct ttttcactac
     1441 atcttctata gcaaaatact cattttcttt ttcgttataa aaaacaatgg aactaattaa
     1501 tacctcgtta ttttcaatat caatactaca catcataggg gatttctcat cattatgtat
     1561 tattaattcg cccagtacta ttgaaccgtt taattcttct gtgaaattta tagtttccac
     1621 tggttttacc cacgtttcat cgcactcttc atctattgcc cattcccata tcgggttttg
     1681 tttcaagtct tcaatagtca attcatcaac tggtttgctt ttattcatat acaattactc
     1741 cctttatcat aataaatggg tgattaaaaa aattctttct ttaactgaac aatatggcca
     1801 tttgcatccc tttgaaatac aagttttcgc attttcataa ttagttgaac gccaataaac
     1861 aggttcaatg tagagtttat tccaattgat attattttaa ctggtttcga aatactgtgc
     1921 tcatcctaat aacctctcga acacttgttc ttacggtaat tttaacaatg ttttgagtag
     1981 gtatcaaata gttattaact gacctgattg aaaagaaaaa aggattgtta atgtatgtgg
     2041 aaaagagcga aaaacatcaa gaatgaacaa gaagatttta gtataatatg gatatattca
     2101 atgtgagggg gaatttaatg tgtatcaaat aaaggggtat ttttcgtcac taaaaggaaa
     2161 tcattatgaa attggtaagc aacaaggaga attcgtaaag aaacatccgt atcttattcc
     2221 acaatttata cagcaagaaa atttaatatg ggataaccat tggacagaat ctagaaatgt
     2281 actaaaacaa tattgtccag gaattaatga agaaattgaa ggcttttgcg aagtattgaa
     2341 aattccttca aggaatatga tgtattatta tcagacatta ttaaaagcag gttgtagtca
     2401 ttgtgcagta ttacctaaaa aaactgattt aaagcataca tatgtattaa gaaattttga
     2461 tttatcaccc aaaatagatg atatgcgttt ttgttcgacg catgttgaag gtgcatatgc
     2521 acatagtggc ttttctactc aatattttgg ccgaacagaa ggagttaatg agcatgggtt
     2581 atctattaca ttttcagcat gtggtcaacc tgtagggaat attgaaggtt taagaaaacc
     2641 gatggtaaat ggtttgcaat gttttgcggt aattcgagta atattggaga aatgtagaaa
     2701 tacgcatgaa gctacatctt taataaaaga aatgcctata gcaagtaata ttaatataat
     2761 tgtagcggat cctttaaacg cagcacatat tgaaatattt gacgggcata aatcaataac
     2821 aacaattaat gaggaaagtc aagcttttac cgtatcaaca aaccatgcag tcagttcacc
     2881 tattcagaaa ttaactaata ggaaattaga acaatctaca aaaagatatc acattttaca
     2941 tgagcattta aatcgaaatg aacaggtgag catggaatct ttaaaaggat tagtagaaga
     3001 agaatatcct atcggggtga cggtgcataa ttatgaagag tggtttggga ctttgcattc
     3061 tgtattaatt gatttgcagg atcgtacatt gaatatttgc ttcggatcgc cacttttaaa
     3121 cgagtggtac tcattgaaag ttggagaaag ccttccgttt tccgaagtga atgtgaactt
     3181 taaaaataaa acttatgctg acttttggag agaagagaaa aatgaaatga ctcctaaaga
     3241 ttaaaaagga ataagatggt gaaatgaagg ggtgggaata gacaggaggg attgccattg
     3301 ctacaagcta tgttaccaat tgttctaatc ctttttctag tcgctttgat gtatataaca
     3361 tatcaaaaaa gaaaaaaagc ggggataaaa ggattaaaaa gttcattaac ttctatttgt
     3421 tgctttttaa ttgcaacctt aaatttgttt gcatattggc ttcattttgg cggtattttt
     3481 acttggctag tctcaatcgt attgttgcta gtgggggcgt attttacaaa atatattcca
     3541 atctcaaaaa aggtgcacta aaattacatg agtttatatg taatgtttta tttcaatgtg
     3601 ctatagatgc ctttaaatca atgcttttga gcatttcgat atgtgtttgc aaacattttg
     3661 taaacacata tcgaaatata ctcttcgcga atgttttcgc tgcatcttct tgcatattgg
     3721 gtaagacatg agaatatacc cttaaagtta aagagatatc aaatgccaat gacagaattc
     3781 atgaagtttg taaagtgcga gaacgaaggt gtttttgaca tcaagcagat gtacagcgct
     3841 gaaaaagctt tcaagaaaat gcagaaccac cgaaatctac attttgctta catgggtatg
     3901 cgattaaatg tagctggcaa gtggggtact atcgttggaa aatgaaaggt gacgataaga
     3961 atcatgagat acgttttaaa caaattgaac ggacattgaa atatgctctt gataatgatc
     4021 aacgtcagat aattgagttg aagtattttg ggagtgagaa agtcaaagat tcctatgttt
     4081 ataatgaatt gatgatgagg cgtgatagtt tttatgagaa taaaaaaata gctatccgtt
     4141 tgatagctac tgctttaggg attatatgaa cgtggtttta ttttagtgaa gatgacatta
     4201 tgatcttttg tgttactgat ataaattgtt atatgatacg tgtaatccat cttactcgct
     4261 ctgccttgtg gcagggcttt tttatataat aatataaatt aagtagttaa agaggtgtgt
     4321 ttaaatgagc tgggaagaat catatagaag agagcaacca tgtccatgtg gtaagggaaa
     4381 atatattgaa attatgaagg tgtatgcaat agaagaaata taaaaaagtg ctaacacgta
     4441 tgcggaatag acataaaaca tcgctgattt gccattcata aaaatagact tttcttagca
     4501 gaaagcacta attataaata cttataaaat tagctctgat ttagcagtta ttgaagcgaa
     4561 gaaaaataat gatgggagtg catatttgaa atacgaagat gaccaaacaa aattatggaa
     4621 tatacgacta tagaactaga aaatgataac ataaatctaa atgttattgt ttaaattcat
     4681 attttataat ttccatttgt aaaaatgagc ccatagtaaa aacaacctaa aaataggtat
     4741 atgaacaagg tacatcatct agtgataggt catattttgt aggtgtacgt attgaactta
     4801 gtttaacccg ctatttgtgg gcagtaaaac tcccacctca aaattcgact ggagcaaaga
     4861 agttagatgg gagtcgggct gcccgtaaac gccccactga ttaaagtttt actttatagc
     4921 gctatttcta cttagattta acgagcagaa atataggaaa gcatcgatgg ggttgtagtt
     4981 tataaacgga ggtcatgaaa atgaataagt ttcaacaaga actacaagcg ttaagcctta
     5041 atgattatca gtctggaaat attgtgtatt gggaccagca aaaccaatat ccatattact
     5101 atattataga tgatgctcgt cgctgtggcg gttgtggtcg ttgtggtggc ggtggtcgtt
     5161 gtggtggatt ccgttgtttt ggttgcttcg gttgctttag ttgttttggt tgtggtggtt
     5221 gctctaattg ttctaattgc tctaattgtt ttgatggttt taatggtact actgtaacat
     5281 ttgaatattg ataagagaca aaaaggaatc cattttgaaa aagattgatc gaaacggatt
     5341 cctactttgt gaagtattat ataattttta tgaaaatgta ctaacaagtt tgcaaacgga
     5401 cttgctaaca ccaatacgaa atagacataa taacaccaat acgaaataga cataaaatat
     5461 cgccgatgtg tagcttaata atacgataat aatttagcac ttataatgaa taataggtta
     5521 ttagaaaaga aaccataaaa tgtaatgttt tatatcatta taaaaagaga ttattctaat
     5581 agggcgttgt gtaacggctg cataggataa tctctttgta tgtttaattt tgtagagaat
     5641 gtactgttaa ctttagttgt tcaataaatt ctcgggtagc agcgcacata tatttatctt
     5701 tcctataaat aagtcctata tctattgtcg gagtaggatt ttcaatttta atagcttgta
     5761 tgtgctcatt ttgcaaaaag tctatatacg gttttggtaa cacggtaatc cccatacctt
     5821 gtgcgaccat ttggattaaa gattccattg tactaatttc taaaattggc tttggtttaa
     5881 agttaaagtt ttggcaatga gatgtaatta gttctgttaa atagaaattt ttaggtagaa
     5941 gaattaaagg atagtcctgt aattcttcaa tagatacata cttacgttca gttaaagaat
     6001 ggctggttgg cacaactaat gtaagttcac ttttatatag tgggatagaa acaatctctt
     6061 tatcttgcac cgggagaaat gtaataccaa tatctaattc attttgtaat aatttttcac
     6121 gaatttctcc tgtacgaagc cccaacacag agagttcgat atttggatat tgcttattga
     6181 aatctaatat agctggtggt agtaaataat ttactacggt taacaaagat cctattgtta
     6241 aggaaccatg atgtaatcca tttaaatctt gaatggcaga acgagcttgt tcgacttcgt
     6301 gaaagattgt tttactgtgc gataataaaa ctcttcctgc ttcagttaaa gatatttttt
     6361 taccaattcg atcgaaaagt ggcatgccta cttcatgttc taatgcacgt atttgttggc
     6421 ttagtgaagg ttgtgaaata tttaatttct cggctgcttt tgtgaaatgt aattctttcg
     6481 aaacagctaa aaagtattct aattgtcgta attcgattga aatcacctca agtttaatca
     6541 taggatatac ctatcattat tatataaata attgaattga acaatggttt tgtgggatat
     6601 ataatcactt tatcagaaat ggattgatta aaaaattaga tgaggtgaag aagaattgtt
     6661 gaaatctagt gtattaccgg gcagttattc acaagtactt caaaaaaggt atgaagaggc
     6721 tgtaccgaca ggaatatatc atttaacgcc tttatatgta aaagaagcaa ggggagcgat
     6781 aataacagat gtagatggaa atcagtttat tgattttgct ggtggaattg gtatgcaaaa
     6841 tgttggacat tgtcatccga aagttgtaag agcggtacaa aaacaagcag aatcatttat
     6901 acatccttgt tttcatgtaa ctccatatga aaattatatt actttagcgg agaaaattaa
     6961 tgagaaagtg ccaggtacaa gtaagaaaaa aacgatgttt gccaatagtg gtgcggaagc
     7021 gattgaaaat gctgtaaaga tcgcacgtaa agtaacaggg aggagtaaag taatttcgtt
     7081 tgaacgagga tttcatggga gaacattaat gacaatgtct ctatcgacca aagaaactcc
     7141 atataaagca ggatttggtc cgttcgtttc agacatgcat atattgccgt atccgtacta
     7201 ttacagagct gaagaaggag tttctcctga agaagtggat tctcaagtca tctatcagtt
     7261 tgaaaagttt ttagaagaag tatcacctga aaaagtagca gcgatcgtaa tggaaccagt
     7321 acaaggagag ggtgggttta ttgttccttc catttcattt gtaaaacgat tacgagaaat
     7381 ttgtaatcaa tacggtattt taatgataat ggatgaagtg caaactggat ttggccgaac
     7441 agggaaatgg tttgcaacgg aacattttca tatagaacct gacataatga ctttgtcaaa
     7501 atcaattgct ggcggtttac cattaagtgc tattgtcggt cgtgcggagt tgatggacat
     7561 tcctgggaga ggacaaattg gtggaacttt tgccgggagt ccgttatcat gtgcagccgg
     7621 tcttgctgta tttgaaataa tggaagaaga aaaactagtt gaaagggctc agcaaattgg
     7681 aaatagaata atgtcacatc tacatcgaat gcaattaaag tacgaagtga ttggtgatgt
     7741 tcgtggaatc ggtgcgatgg ttgcaatgga acttgtaacg aatcgcataa cgaaagaacc
     7801 tgcaaaagaa ctaacagtaa agctaattga agatttttgg aagaatgggc ttataagtat
     7861 tggagcgggg atatttgata atgtactacg ctttttacca cctctagtta tctcaaacga
     7921 agaaatagat agaggatttg aaataataaa tgagtctttt gaaaagttgt gccgcaagta
     7981 agaggaggga tttgaatgac taaagtatat gaaatcctat ctttaaatct aggattgcca
     8041 aaagaggtta tgtatggtgg gaagttaatt caaacaggga taaataaaaa gcaagtaaag
     8101 gaacctgttt ttttatcatt tttaaagttt aatggagatg gacaggcgga tttagttcat
     8161 catggagggg gagataaagc ggtttgtgtt tatactggtg accactatcc ttactgggaa
     8221 aaagagttga atcaggaact tgtatatggg gctttcggtg aaaatataac agtaagcgga
     8281 atgcgtgaag aagatgtttg tattggtgac acgtttcaac tcggagaagc gattgtacaa
     8341 gtaacgcagc caagacaacc ttgttttaaa ttagcgaaga aatataatat tccaaagttg
     8401 ccattatatt ttcaagatac aggatacaca ggtttttatt ttcgagtatt aaaagaaggg
     8461 tggatattac cggtagatac tttaaaattg ttgaagcctg atccaaaagg tgtgtcaatt
     8521 ttgtttgcga atcgcataat gcataaagaa aaacaaaact ctgaaggatt aaaaaggatt
     8581 ttagaagtaa atgcactgtc tactagttgg agaaagacgt ttgaaaagcg agtgaaggga
     8641 gaagaaatta gtacgaagga gcgacttgag ggaaccaaag aataggtgaa ttatatttgc
     8701 ataaaatggt gtatttctat tatcggaaag ataagtagga agcaccattt gttatataat
     8761 aaacatgata ttattcaaaa atcttaataa tagtttattc gacttgtttt ttaataatca
     8821 aaacataaaa taaattaaac catttttaaa agttttatga tactatattg atttgaaagg
     8881 cctatttttt gagagtaggt catataagta tatctaaatt aatggaatcg acgtatggag
     8941 ggacaacatg gggaatgttg aaaaaatggc aaactttcta ttagaagaac cgagaagaga
     9001 acagttattt cctttatttg aagaagtgtt tgggattacg agccgaacat tgcaggattt
     9061 ttcagagaaa gggtattggg attatacata taaagcatta tcatttttac aagatgataa
     9121 tgcgattgca aatgttgcgg cattttcact tccgctttta attaatggtg aaaaaataaa
     9181 tgcaacgggt attcaatcag taatgacaca tccgaattat cgtagacgag gattaatgaa
     9241 acaattgttt agtaagatga tagaggaaat tgatgagcaa tgcgagtgtg cggtcttgtt
     9301 tactgaaaat ccggaactat ataaaccatt tggttttcaa attgtgcaag aatatttgat
     9361 gacagtatca tacgaaaata aaggttacaa agattctcaa cttaaaaagt tagattttta
     9421 taatgaagag gatagacaat taatacatga gattattgaa aatggccaaa ggctttctaa
     9481 taaattttca acgttaaatt accgctcttc gttttattta aatatgtatg attcaacatg
     9541 gaatgaaaac ctttattatt cagagaaatt agatgcgctg attgttttta cagtagaaaa
     9601 tcaaaaacta aaattatttg gagtatttgc gccggtaatt ccgattttag atgaattatg
     9661 tgaggaaatc tctggggaat ttacggagat tgaattttat ttctacccag atcaattagg
     9721 aattgatgat gtacaatata cagaattaca gactagtaag tatttaatgg ttcgaagtaa
     9781 taaagaatta gattttaaag gttataaatt cccggtatta actgagtttt aaatattaat
     9841 ttaaatgctt ttttatggag gaattatgaa aacatcatat ataccaaatc atattgagca
     9901 acttactgta atgaacgcac ctaaatttta caaacttgaa gacaatgata cattcattgt
     9961 ccattcgaaa gaagaaacag atttttggct gaaaacccat tatggttttg aagtaatgaa
    10021 tggccatgta ttttatgatg agataatgac agattttcag gctgaggttc aattgcgcat
    10081 gaatccaaat gcgacatacg atcaagcggg tttatttgtt atgatttctg agaagtgttg
    10141 gttaaaaaca tctttagaat atattcctga gggcccatca catttaggtg ctgtcgtaac
    10201 gaataatggc tattctgatt ggtcaacgca agactatcca aatgagtttg ctaagcaaca
    10261 attacgtttt cgaattattc gaaaacttgg c
//
