LOCUS       NZ_CP092907            20729 bp    DNA     circular CON 07-APR-2024
DEFINITION  Enterococcus faecalis strain LJX 0909 chromosome, complete genome.
ACCESSION   NZ_CP092907
VERSION     NZ_CP092907.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecalis
  ORGANISM  Enterococcus faecalis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2812373)
  AUTHORS   Liu,R. and Fan,W.
  TITLE     Metformin significantly reduce the alkali resistance of standard and
            wild E. faecalis in normal and high glucose environment
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2812373)
  AUTHORS   Liu,R. and Fan,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAR-2022) School and Hospital of Stomatology, Wuhan
            University, Luonan Street, Wuhan 430075, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unlcycler v. V0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 255.1x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022557275.1-RS_2024_04_07
            Annotation Date                   :: 04/07/2024 08:03:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,843
            CDSs (total)                      :: 2,765
            Genes (coding)                    :: 2,717
            CDSs (with protein)               :: 2,717
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 24 of 48
            Pseudo Genes (incomplete)         :: 17 of 48
            Pseudo Genes (internal stop)      :: 16 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 15:20:35
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1566534
            Orig. end                         :: 1587263
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP092907.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..20729
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="cyclic-lactone-autoinducer"
                     /region_number="4"
                     /rules="(TIGR04223 or AgrB)"
                     /tool="antismash"
     cand_cluster    1..20729
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(TIGR04223 or AgrB)"
                     /kind="single"
                     /product="cyclic-lactone-autoinducer"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20729
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:10729](-)"
                     /cutoff="20000"
                     /detection_rule="(TIGR04223 or AgrB)"
                     /neighbourhood="10000"
                     /product="cyclic-lactone-autoinducer"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..10729)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(TIGR04223 or AgrB)"
                     /neighbourhood="10000"
                     /product="cyclic-lactone-autoinducer"
                     /protocluster_number="1"
     misc_feature    108..123
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: strong, score: 28.99"
                     /tool="antismash"
     misc_feature    579..593
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 19.08"
                     /tool="antismash"
     gene            complement(1998..3386)
                     /locus_tag="MK744_RS07590"
                     /old_locus_tag="MK744_07590"
     CDS             complement(1998..3386)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1005: Drug
                     resistance transporter, EmrB/QacA"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010712556.1"
                     /locus_tag="MK744_RS07590"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MK744_07590"
                     /product="MFS transporter"
                     /protein_id="WP_010712556.1"
                     /transl_table=11
                     /translation="MIDRKKVILYTCCMSLFVVTMDVTVVNVALPSIQSDFHTNLSTLQ
                     WVTDGYTLMVASLLLLSGSTADRIGRKRVLQLGLACFGLASFLCGISQTPGQLIAFRML
                     QGIGGSMLNPVAMSIITQVFTEKLERAKAIGLWGSVTGISLGMGPIIGGLIVSYFSWRY
                     VFFVNVPIIAAAIILTQKFVPESKVEKTAKNDFVGQALMILFLFSSIYSIIGLPRKGLF
                     APDILSTGIIGCLAIVIFFIYEYNIDNPLINPRFFLSIPFTSASFLAIFGFIIYNGYLF
                     LNTLYLQEMRGFSPLEAGLSTIPLAFVSFLVAPRAGEMVGRIGTKRPIMLCGISMLAVS
                     FLQLFVTKTTPMIILFVIYIFLGIGFGMLNSPITITAIEGMPLSQSGTAAAIAVTCKQI
                     GNSLGVALPSLLITKPITSSLTRTPFTNVWLLFGCCAIAIIFLSYLSNSPLAKKSLRRV
                     RFYF"
     misc_feature    complement(3648..3664)
                     /note="TFBS match to NtrC, Nitrogen regulatory protein C,
                     confidence: weak, score: 20.68"
                     /tool="antismash"
     gene            complement(3768..4622)
                     /locus_tag="MK744_RS07595"
                     /old_locus_tag="MK744_07595"
     CDS             complement(3768..4622)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1014: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821679.1"
                     /locus_tag="MK744_RS07595"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MK744_07595"
                     /product="LysR family transcriptional regulator"
                     /protein_id="WP_002357233.1"
                     /transl_table=11
                     /translation="MTIEKLEYFYTIAKYNSISKAASELHVSKSTLSASLKDLESELGH
                     LLFDRNGNSLTLNSYGDKIVQSVYIILNEAKKMKLNLHEMIENPVMRLGFGNTSLMYKV
                     TENEDQLNRFWECYHGSSFELLNKLENHELDFVITSADVNSPVLKKEQLIELKMYLCVS
                     REIKQEIEEEGFTCLTNYPFLFLPHHLDHLEATKSVLEMLQLTSPLVCCYDTLMLTRLI
                     EKSKGVYAVISLRKEQLQEIDSKLFFLPIERKQKFYLYRNVSSSVFVQPGQIKATLQKL
                     LET"
     gene            5013..5843
                     /locus_tag="MK744_RS07600"
                     /old_locus_tag="MK744_07600"
     CDS             5013..5843
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002397393.1"
                     /locus_tag="MK744_RS07600"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MK744_07600"
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="WP_002369253.1"
                     /transl_table=11
                     /translation="MKINYLGTGAAERIPGIFCNCKLCKYAREKKGREIRTQTQLIIDD
                     VLLIDFPGDSYLHLLQYDLCFADFDSLLISHWHSDHFYGEDLAYRMSGYALDIPNRLTV
                     YGNEAVKTFYQRAFDLEGRQDDTRLHYQVIQPYKCFTIANYTIYPLPAQHGNFSEDCFI
                     FVIDDGKDAFLSTHDTGYFTSEMFEYLEKSHLLLSIVSLDCTSQTNETGNSHMNWEENL
                     KLIAELKERKLVTDKTIYVANHFSHNGGLSYAEMAALSQKHEIITSYDGLEILT"
     gene            complement(5991..6845)
                     /gene="sprE"
                     /locus_tag="MK744_RS07605"
                     /old_locus_tag="MK744_07605"
     CDS             complement(5991..6845)
                     /codon_start=1
                     /gene="sprE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364098.1"
                     /locus_tag="MK744_RS07605"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MK744_07605"
                     /product="serine proteinase SprE"
                     /protein_id="WP_002379530.1"
                     /transl_table=11
                     /translation="MKKFSIRKISAGFLFLILVTLIAGFSLSANAEEYIVPAESHSRQK
                     RSLLDPEDRRQEVADTTEAPFASIGRIISPASKPGYISLGTGFVVGTNTIVTNNHVAES
                     FKNAKVLNPNAKDDAWFYPGRDGSATPFGKFKVIDVAFSPNADIAVVTVGKQNDRPDGP
                     ELGEILTPFVLKKFESSDTHVTISGYPGEKNHTQWSHENDLFTSNFTDLENPLLFYDID
                     TTGGQSGSPIYNDQFEVVGVHSNGGIKQTGNHGQRLNEVNYNFIVNRVNEEENKRLSAV
                     PAA"
     gene            complement(6894..8426)
                     /gene="gelE"
                     /locus_tag="MK744_RS07610"
                     /old_locus_tag="MK744_07610"
     CDS             complement(6894..8426)
                     /EC_number="3.4.24.30"
                     /codon_start=1
                     /gene="gelE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369251.1"
                     /locus_tag="MK744_RS07610"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MK744_07610"
                     /product="M4 family metallopeptidase coccolysin"
                     /protein_id="WP_142957143.1"
                     /transl_table=11
                     /translation="MMKGNKILYILGTGIFVGSSCLFSSLFVAAEEQVYSESEVSTVLS
                     KLEKEAISEAAAEQYTVVDRKEDAWGMKHLKLEKQTEGVTVDSDNVIIHLDKNGAVTSV
                     TGNPVDQVVKIQSVDAIGEEGVKKIIASDNPENKDLVFLAIDKRVNNEGQLFYKVRVTS
                     SPTGDPISLVYKVNATDGTIMEKQDLTEHVGSEVTLKNSFQVTFNVPVEKSNTGIALHG
                     TDNTGVYHAVVDGKNNYSIIQAPSLATLNQNAVDAYTHGKFVKTYYEDHFQRHSIDDRG
                     MPILSVVDEQHPDAYDNAFWDGKAMRYGETSTPTGKTYASSLDVVGHEMTHGVTEHTAG
                     LEYLGQSGALNESYSDLMGYIISGASNPEIGADTQSVDRKTGIRNLQTPSKHGQPETMA
                     QYDDRARYKGTPYYDQGGVHYNSGIINRIGYTIIQNLGIEKAQTIFYSSLVNYLTPKAQ
                     FSDARDAMLAAAKVQYGDEAASVVSAAFNSAGIGAKEDIQVNQPSESVLVNE"
     gene            complement(8661..10004)
                     /gene="fsrC"
                     /locus_tag="MK744_RS07615"
                     /old_locus_tag="MK744_07615"
     CDS             complement(8661..10004)
                     /codon_start=1
                     /gene="fsrC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002402207.1"
                     /locus_tag="MK744_RS07615"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MK744_07615"
                     /product="sensor histidine kinase FsrC"
                     /protein_id="WP_142957144.1"
                     /transl_table=11
                     /translation="MILSLLATNVLLASSFIVFVFLRVTLIKIECKIPLLSLLIVINLC
                     SFAALMLGYSWLIYALTVVVFTGFLLIHKKRFSIFKAIFLSVFTLLMVSFINYTEQTIL
                     SVFFQQIYQNKLLWIASNVLLLLINIWIALKIPNSVFLRLNRVLENSRIFFGCLLLLLI
                     LLLLFVFLISPEISPDFMRGFVTVNSSKLELLISVGLFLILIGLVIEAYLEEQRINTQL
                     LNNLTIYTEKIESINEELAMFRHDYKNLLYSLQIAISYEDILEIKRIYEETIAPTKKII
                     DNEEFELMKLNRLKNMELKALISMKINTAKQAKLKVIVDVPEVFILDTSIDLVVVIRLL
                     AILLDNAIENSAKSELKMFAISIFNKDETQEFVITNSVQAEFDFKVMKKTKFSSKSNPE
                     EHGWGLLYVKEIVDFSDQFDLQTSFKEGSVTQHLIIEKNHNSKKVVNE"
     gene            complement(10001..10729)
                     /locus_tag="MK744_RS07620"
                     /old_locus_tag="MK744_07620"
     CDS             complement(10001..10729)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     cyclic-lactone-autoinducer: AgrB"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634271.1"
                     /locus_tag="MK744_RS07620"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MK744_07620"
                     /product="accessory gene regulator AgrB"
                     /protein_id="WP_002377181.1"
                     /sec_met_domain="AgrB (E-value: 1.1e-31, bitscore: 106.9,
                     seeds: 181, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLIDWILKNIMDMDQEDQSGKTQWTKYYLTVYFSGLFNFLMILIL
                     SVLFGTLSETFIVYVVLIFLRPVAGGWHAKTKWLCRLESIVIYVAIPFVLKNSSVSLPF
                     IYKILLICLLVVLFYWYAPQGTAIEPVQPSDLNVLKKQSLIRVCLLILCSLFVKEKIAS
                     VILYGLVIQGLMILPVTKNLIEGSVFMKFGKKIIKNVIEKRVAKVSDGVGTKPRLNQNS
                     PNIFGQWMGQTEKPKKNIEK"
     gene            complement(10818..11549)
                     /gene="fsrA"
                     /locus_tag="MK744_RS07625"
                     /old_locus_tag="MK744_07625"
     CDS             complement(10818..11549)
                     /codon_start=1
                     /gene="fsrA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011109506.1"
                     /locus_tag="MK744_RS07625"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MK744_07625"
                     /product="response regulator FsrA"
                     /protein_id="WP_142957145.1"
                     /transl_table=11
                     /translation="MAIYILEDQIIQAKALEALLTNILHSRNIYNETIHLFSRSDELLQ
                     VAHQDAQLNIFFLDIQMNNHIQAGFEVAKEIRKTDSESLIVFISTHTELVLTSYKYMVS
                     ALQFIQKNVDFLDFQKEVETCVDTYIQQKENIKTKSEYIIINLKASSIKMDINDIYFFQ
                     TEYDHRVSMVGKNFKREFYGTLSKIEQLHPDLIRVHQSIIINKKYASKLNYKTHLLTMR
                     DGTEVPVSRRYYTQVKALFLT"
     misc_feature    11662..11677
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 17.55"
                     /tool="antismash"
     gene            complement(11969..13972)
                     /locus_tag="MK744_RS07630"
                     /old_locus_tag="MK744_07630"
     CDS             complement(11969..13972)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401391.1"
                     /locus_tag="MK744_RS07630"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MK744_07630"
                     /product="amidase domain-containing protein"
                     /protein_id="WP_241408173.1"
                     /transl_table=11
                     /translation="MKRKKIKNQLLVLTSILVLLSLEVAPVVTLAAELPSSSLQTALSS
                     KTTITSEEKVIETTKTTETTETTETTETTETTVATSTTSTSSSSSESNSSTDTTTESTS
                     QSTTETTTTNTSSETKKEPTEPTVSSEITQPVEQSQPPQVPVTKQEPEEPIQVPEATNN
                     VVEENQAVSLNPSLKVDEIASSNLKGYELPLLSSFGEKKRAVVVAEALRHVGKTKKEFN
                     LTEQALTSSFLAQKIYQQLFKIDIGSTPQEQMTFGKVRSIEEAEPGDLIFWQTAEGKTL
                     QNGVYLGQGKYLIAAAEADSKEEPEVIAQLENIYTAKQQPDSKEEIRLVVTNPFKEFTL
                     TEYGKEVLATYGASFEMQKSEQTTAFIKKIGETARELGEKYDVFASVMIAQAILESGSG
                     ESQLAKEPYYNLFGVKGSFQGNSVSFSTKEADQRGQLYTISAGFRDYGGYNDSLQDYVQ
                     LLRQGIDGNQDFYKPAWRSEAKNYLQATRFLTGKYATDKQYDNKLNSLIAVYNLTQFDL
                     PKTVDGLIIQSKNKLSEAEQQQMHFPVYDGINYNRSGSYPVGQCTWYVYNRFKQLGTSV
                     DEFMGNGSDWGRKGRALGYQVSSLPKAGRAISFQPGVAGADNQYGHVAFVEAVTSDGII
                     ISESNVINDQTISYRVLPNVIAYSSGVTYIGA"
     gene            complement(14054..19681)
                     /locus_tag="MK744_RS07635"
                     /old_locus_tag="MK744_07635"
     CDS             complement(14054..19681)
                     /GO_function="GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016633936.1"
                     /locus_tag="MK744_RS07635"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MK744_07635"
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="WP_185938075.1"
                     /transl_table=11
                     /translation="MGSNFKEEKMNKEYQGKKIYGKKHWLTFLLVSSSVLAFHSFVTMD
                     SQVVDAQEQTAKEDVADSATSVGAIVSIEKAEKNFVITYASGKKAQISILNDHLFRYHL
                     DPTGKFEEYPTPNDPKHVAKITAKTMADYGTQAFEQTNVTDSGNQFILENNGLKIMFEK
                     ESALMKVLDKKKDQVILEETAPLSFKNDKATQTLKQSSQENYFGGGTQNGRFTHKGTAI
                     QIVNTNNWVDGGVASPNPFYWSTAGYGVVRNTWKPGNYDFGSHDPQTTTTTHEGTDFDA
                     FYFFNDSSAGILKDYYELTGKPALMPEYGFYEAHLNAYNRDYWVKVAEGTAGAVKFEDG
                     NFYKEYQPGDLGNLNGTLESLNGEKENYQFSARAVIDRYKKNDMPLGWFLPNDGYGAGY
                     GQTDSLDGDVQNLKEFTDYAQANGVEVGLWTQSNLHPADPKNPKKGERDIAKEVSVAGV
                     KALKTDVAWVGYGYSFGLNGVEDAANVFVKETDGAVRPMIVSLDGWAGTQRHAGIWTGD
                     QTGGQWEYIRFHIPTYIGTSLSGQPNVGSDMDGIFGGKNKEVNIRDFQWKTFTPVQLNM
                     DGWGSNPKTPFAFDQEATDLNRAYLKLKSMMMPYNYSIAKESVDGLPMVRAMALEFPNE
                     GTAYTKDSQYQYMWGPNLLVAPIYNGNQDEAGNSIRDGIYLPDEKQVWVDLFTGEKYQG
                     GRVLNGVKTPLWKVPVFVKDGSIIPMTNPNNNPKEIQRDQRSFLIYPNGATSFNMYEDD
                     GISTSYEAGQSATTKINSQGPKSNEKGDLTVTIEPTKGSYSGFVDERSTTLDLLASEAP
                     ESVTAMVGGTEVTLKQAANKEEFLAGTNLYYFDKEFQVNQYLSEASGEKLNQSALSVKL
                     AKQSVTAKDVQITVKGFINKGTVDGGNTTVDDQLTIPANVAINEEKTTPSSLTLQWDQV
                     TEATSYEVERDGTVFGNIQTNTATFDGFSFLSEHTFRVRAVGKNGVSEWSEPIKGKTQD
                     DPYKETINQVKATSNLPEQPGAELKKLTDKDLSTGWHTNWSTGIANPSDGNFLSLKFDL
                     GAEYQMDKIEYLPRDNAGNGNILQLQYRTSKDGANWTEFSEPINWKQDALTKTIETKDQ
                     AYRFVEMKVLKSVGNFGSGREMLFYKQPGTEGILHGDITNDGTIDENDAMSYRNYTGLE
                     SVDSDFNGYVEKGDLNKNGVIDAYDISYVLRQLDGGIEIPDVEEIAGGLSLAVVNENGK
                     DTYLPGDTLTFTLKGQDLKNINALSTKMSFDSSKFELVGQPATTNNTKQMENYSKYRKH
                     SNDVENLYLVLSNQGNKQLLNGSMDLVTFKVKVKETTRVKRATTVEQPLQFDMSQGLLV
                     GQGFQQATLSDFSVTVKPTELVDKELLQALITLNQARVEKEYTPETWAIFKPILDEAVA
                     VLANEQATQTDVSAAVENLEKAVEQLEKMPDVANKADLEKAIQEGLAKKPSDGQEFTEE
                     TKKVLEESLAAAQKVFAQEKVTQEEIDQATKTLREAIAQLKEQPVAVDKETLKEKIAQA
                     RGIKPEEGYQFTKETEKQLQEAIQAAEAIVAKETATKEEVSAALNALETAMAQLKEVPL
                     VNKDQLQEVVKRAQQVTPSEGHQFTASSLQDLQKALLAAKNTLENPAANQKMIDEAVAE
                     LTSAIDGLQEEALVTDKKALEAMIAKAKAIKPSAGKEFTSESKARLTEAIDQAEGILDD
                     KNARQEQIDTAEKNVKTALDSLEEQVLQTDKTKLKELLQKAETLKPKAGKQFTKASQEA
                     LAEAIKQAKALVEDPSATQEAVDKCLSILAQAIEAMAEEPISSNSTGNNGNHGTVSGTG
                     SVTSTGKGTATGGTTTKTSTSGTLPKANEVVSPIWSISGFLLIVSIGLGKLFFKNKKDQ
                     N"
     gene            complement(20185..20580)
                     /locus_tag="MK744_RS07640"
                     /old_locus_tag="MK744_07640"
     CDS             complement(20185..20580)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407764.1"
                     /locus_tag="MK744_RS07640"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MK744_07640"
                     /product="hypothetical protein"
                     /protein_id="WP_002361986.1"
                     /transl_table=11
                     /translation="MDGMFANLTLRERRLLKQAQVGATISFILLFVPILTISLLHTSDL
                     LAKWLGIVGVIMVLPLLYFAWQILKIAYKDQKDNPTPPLWVPKVYGIGLSINPYHRFGK
                     LIFILLAVLIVGIIISLLFTPASQINH"
ORIGIN
        1 tttttcaaaa gctacatcat agttgtagtc ctttggttgc acttcttgga atccttgtgg
       61 tttataaaaa cgtaaaaggt tttcctgatt taatttgtca gataaactta acgatttttt
      121 cacccaatcg ttatctttcg tgacttctgc ttttaaatct gctggaactt ggtctaattt
      181 ttttccttga ttattgtaga tttccccacc tatataagaa aattgaggcg ttacaaaatc
      241 gccatttcta aaaggaacca cttgtttata ttgaggagat aaaaggtccg tgccaaaatg
      301 gatataattt ttatcatcga cacctaaaag atggtacagt gtcggtaaaa cgtcaatttc
      361 cccaccatat tcgtgcatga ttttcccttg cgtatagcct ggcatgtaaa acatcaaagg
      421 tactctttgc aattgcgcat tgtcatagtt tgtccacgtt tttggatcgc ggttgaaggc
      481 tttacataag tccttgttgt tcgcatctga aataccgaag tggtcaccat aaataacaaa
      541 aatagaatga ttgtataaac ttgttgtttg caagtaatgg aaaaattgtt ctagcgattg
      601 atccaaatag tgcgctgttc gaacataatc atccacaatc gaattgcctg tgttcaattt
      661 aggaaacggg aagttttggt catccgtata ataaggggta tgattggtca cagttaaaaa
      721 cttcgcatag aatggctgct gtaaatgttc aagctcacta gctgattcac gcaacaattg
      781 tttatctaaa ataccgtaac ctagcatttt gtcatctttt gaaaaatatg accgatcaaa
      841 aaagtattga tatcctaagt ttttatacac atggtctcga ttccaaaaac tccccacatt
      901 tccatggaaa acagcggttg tatagccttt ttgtcctaaa acagcgggag ccgcttcaaa
      961 agtattgtct gatcctaatt gtgtaaaaag agagccttgc gggagcccga atgttcctgt
     1021 ttctaacatg ttctccgcat cactcgtttt tccttgccct acttcatgaa agaaattatc
     1081 aaaactgatt gtcgctttgt tttgatagag ctgatttaaa aacggtgtaa cctcttggcc
     1141 ttggactttc atattaatca aaaattgttg aaaactttct aaatgaatca caaatacatt
     1201 ttttcctttg gcaatcccgg catactgcgg attaagcggt aaattatttt gcttggtaaa
     1261 tttcatgatt tcaggtaatt cgtttgaact agcttctgct cttacctcat ttgacatggc
     1321 tgttttgaca ccatcgatgg ctgtgaatgc atccaagcca agatatttta caatataatt
     1381 acggtcaaaa attctttgaa gcaattgagg acgatcggtt tcgcttaaaa ataagttaac
     1441 cgaaaagagc aagacactta aacaactaat tgagatcgcc aatcttctgt tgataggttt
     1501 agtcataaag acttgatgat tccgtcgctg tttaaaagag taaaatacca aaacgagcag
     1561 cactaaagta tctagccaat aaaagacatc gtgaaccctc attaaggcaa gtaaatcacc
     1621 agaaattcct tctgaaactt ttgaaaagcc aaaaacagat tggatggtaa taaagtcagt
     1681 gaattcacga taaaaaataa tattcaaata taaaattaat gtatttaaaa catttaaaag
     1741 agttaagcta agcataaatg ttttatgctt tttaaaatac aaactaaaac caaataataa
     1801 cagcgtagta gcaactggat tgaaccataa aatttctcgc tggagactgc ccttaacgcc
     1861 caatgagaaa tctaggtaat acgccagaat cgtttttgcc caaaagagta aaacaaaaaa
     1921 taaaaagagt cctaatcggt tatttatttt tttcatctaa gtttgtcttc ctttcccttc
     1981 aaaaaaatct tgatttgtta aaaataaaac ctaactctgc gcaatgactt tttggctagc
     2041 ggagaattgg ataaataact taaaaagata atcgcaatgg cgcaacatcc aaataaaagc
     2101 catacgtttg taaaaggtgt tcgagtaagc gaactagtaa taggctttgt aattaaaaga
     2161 cttggtagcg caacccctaa agaattgcca atttgcttac atgtcaccgc aatggctgct
     2221 gccgttcctg actgagaaag tggcattcct tcaatcgctg taatggtaat cggcgaattt
     2281 aacatcccaa acccaatgcc taaaaagata taaataacaa ataaaataat cataggcgtt
     2341 gttttagtta caaataattg taaaaagcta acagccaaca ttgaaatacc acaaagcata
     2401 ataggacgtt tcgtccctat tctccctacc atttcgccgg ctctcggggc aacaagaaaa
     2461 ctaacaaaag ccaaaggaat ggttgataag ccagcttcca acgggctgaa gcctctcatc
     2521 tcttgcaaat atagcgtgtt taaaaataaa tagccgttat atatgataaa gccaaaaata
     2581 gctaaaaaag aagccgatgt aaatggaata gataaaaaga aacgcgggtt gattaacgga
     2641 ttgtcaatgt tatattcata aataaagaaa atgacaatgg ctaaacagcc gattatccca
     2701 gtacttaaaa tatctggcgc gaaaagccct tttctaggta gtccgataat ggaatagata
     2761 gaactaaata gaaaaagaat catcaatgct tgaccaacaa aatcattttt cgccgttttc
     2821 tctacttttg actcaggtac aaacttttgt gtaaggatga ttgccgcagc aatgatcggt
     2881 acatttacaa aaaagacata ccgccagcta aaataagaaa caattagtcc gccaataatc
     2941 gggcccatgc ctaaggaaat ccctgtaaca gagccccaca agccaattgc tttcgcacgt
     3001 tctaactttt cggtaaatac ctgtgtaata atagacattg ctaccggatt taacatagaa
     3061 ccaccaatcc cttgcaacat gcgaaacgcg atcaattgcc ctggggtttg cgaaatccca
     3121 cataggaaag acgctaaacc aaaacaggct aagcccaatt gaagaactcg tttacggcca
     3181 attcggtctg ctgtagaccc agataacaat aataaggatg ctaccattaa agtgtagcca
     3241 tctgttaccc actgtaatgt agacagattc gtgtgaaaat cactttgaat ggatggtaac
     3301 gccacattga caaccgtaac atccatggtc actacaaaca aactcatgca acaagtatac
     3361 aaaataactt tttttctatc gatcataaaa acagtccttt ctagcaacta cataaaacag
     3421 gaaaagcaac aatgacactt aaacttctaa cgacacagtt atcgcaagta ctcacgtatc
     3481 aacagttctt tatattcctt gccatttagt tattttatac ttaaaaaaat aaatctttag
     3541 atagccaaac tatacaccag aaccccttta aattgaaccg taatagatac gatttatcga
     3601 ttcttaagtt attaaataat tgaacattct gttcagaaat atcgaacagc cccataattg
     3661 agcatctctt ttaaacaatg aacaacaaaa atagccttat ccgcagcaga agtaaatcta
     3721 agcgaataaa gctatttttg gtaactattc caaagtaatt ttaatggcta agtctctagc
     3781 aacttttgta aagttgcctt gatttgacca ggttggacaa aaaccgaact ggaaacattt
     3841 cggtataaat aaaatttttg ttttcgttca atcggtaaaa agaagagttt agaatcaatt
     3901 tcctgcagtt gttctttccg aagagagatt accgcataaa ctcctttact tttttcaatg
     3961 agacgagtaa gcatcaatgt atcgtagcaa caaacaagcg gacttgttaa ttgcaacatt
     4021 tctagcacac tcttggtagc ttctaaatga tctaagtgat gcggcaaaaa tagaaatgga
     4081 tagttggtca aacaggtgaa gccttcttcc tcaatttctt gtttaatttc tcttgaaaca
     4141 caaagataca tttttagttc aattaattgt tctttcttta aaactggact attcacatct
     4201 gcagaagtaa tcacaaaatc caactcatga ttctccaatt tattcaataa ctcaaacgat
     4261 gaaccatggt aacattccca aaaacgattc agttgatcct cattttctgt cactttatac
     4321 atcaaggacg tatttccaaa acctaaacgc atcactggat tctcaatcat ttcatgcaaa
     4381 ttaagtttca ttttcttagc ctcgtttaaa atgatatata cagattggac gattttatca
     4441 ccataggaat ttaacgtcaa agaattaccg ttacggtcaa aaagtaaatg gcccaattca
     4501 ctttccaaat cttttaatga cgcacttaag gttgatttac taacatggag ttcagaggct
     4561 gctttggaaa tactattata ttttgcaatg gtataaaaat attctaattt ttcaatagtc
     4621 atcttttgtc atccctttta ttcaaattca tttgcccacg caacctgttc aaaacacgcc
     4681 tcactcttta tcaatagcgt agttattttt gctctaataa gctatgtcca tcttgttatt
     4741 tttctataac taatagcaga tggatatgaa agtgcctttg gtaaaacttt ttttcggaaa
     4801 tagtacgagc attcgcagta aattcggctt atcaaaaaag gcgtctagct ttttctattt
     4861 tacgcatatt tacttaagaa attccatttc aattcttctc taaaaaaggt aagatggaca
     4921 caacaaatgt ttcagtttcc atgcccattt actttaaggt tcagcgttat ctactaagta
     4981 tgagaatgtc aaagaaaggt taggtactcc caatgaaaat caactattta ggtacgggtg
     5041 cagcagaacg gattccagga attttttgta attgtaaatt atgtaagtac gctcgagaga
     5101 aaaaaggacg agaaattcgt acacaaactc aattaattat tgatgatgtc ctgctgattg
     5161 attttccagg ggattcttat ttacacctgt tgcagtacga cttgtgtttt gcagactttg
     5221 actctttact aatctctcat tggcattccg atcattttta cggcgaggat ttggcttatc
     5281 gaatgtctgg ttatgcatta gatattccca atcgtttaac tgtttacgga aatgaagcag
     5341 ttaagacgtt ttatcagcgc gcctttgatt tagaaggtcg tcaagacgat acccgattac
     5401 attatcaggt tattcagccc tacaaatgtt ttactattgc gaactatacg atttatcccc
     5461 ttcctgcaca acatggaaat ttcagtgaag attgttttat tttcgttata gacgatggta
     5521 aagatgcttt tttatccacc catgatacag gttactttac gtcagaaatg tttgaatatc
     5581 ttgaaaagag ccacttactt cttagtattg tttctttaga ttgtactagt caaacaaacg
     5641 aaactggcaa tagtcatatg aattgggaag aaaatttaaa attaattgca gaattaaaag
     5701 aaagaaaact cgtcacagat aaaacgattt acgttgccaa ccatttttct cacaatggcg
     5761 gcttgtctta tgcggagatg gcggctttat ctcaaaagca tgaaatcatt acgagttatg
     5821 atggtttaga aatactaact taatcggatt atttgccttc ttttcagcac tattatatgc
     5881 ttttaaccga taattacaag gtacttttat cgtaacttac actatcttaa aaaacaaaaa
     5941 tactcgagat ttcccgtgat tctggaaatt ccgagtattt ttatggttct ttacgctgct
     6001 ggcacagcgg ataaacgttt attttcttct tcattcactc gattaacaat aaagttataa
     6061 ttcacttcat ttagtctttg accatgattt cctgtttgct taatgccacc attggaatga
     6121 acaccaacta cttcaaactg atcattatag attggtgaac cagattgacc accagttgta
     6181 tcgatatcat aaaatagtaa tggattttct aagtctgtaa agttagatgt aaacaaatca
     6241 ttttcatgag accattgtgt gtggtttttc tcacctggat agcctgatat tgtgacatgg
     6301 gtatctgaag attcaaactt tttcaaaaca aatggcgtta aaatttctcc caactctggg
     6361 ccatctggac gatcgttttg tttgccaaca gtcacgaccg caatatccgc attcggggaa
     6421 aaagctacat caatcacttt gaatttgcca aatggtgtcg cactgccatc tcgacctgga
     6481 taaaaccaag catcatcttt ggcattcgga tttaatactt tggcattctt aaaactttca
     6541 gccacatgat tattggtgac aattgtatta gttccaacaa caaagcctgt tcctaaagaa
     6601 atatagcctg gtttactggc aggggaaatg attcttccga ttgacgcaaa aggcgcttcg
     6661 gttgtatctg ccacttcttg tcttctgtct tcagggtcca gtaacgatct tttttgtcgt
     6721 gaatgacttt cggcaggaac gatatactct tctgcatttg cagacaagct aaaaccggct
     6781 atcaaagtta ctaaaatcag aaacaaaaaa ccagcactaa tttttcgtat ggagaacttt
     6841 ttcataagat tatgccactc cttatccatt tttatttaat tggggaattt ttttcattca
     6901 ttgaccagaa cagattcact tggttggttt acttgaatat cctctttagc tccgattcca
     6961 gcagagttaa aggctgctga caccactgaa gctgcttcat cgccatattg aacttttgca
     7021 gcagcaagca tcgcatcacg agcatcactg aattgtgctt taggtgttaa gtaatttact
     7081 aacgagctgt agaaaatagt ctgtgctttt tcaatgccta agttctggat aatggtgtaa
     7141 ccaatccgat taataattcc actattataa tgaacaccgc cttgatcata ataaggcgtt
     7201 cctttatacc gtgctcgatc gtcgtattga gccatggttt ctggttgtcc gtgtttactt
     7261 ggcgtttgta aatttcgaat acctgttttt cggtcaacac tctgagtatc cgcaccaatt
     7321 tctggattag atgcacccga aataatataa cccatcaaat cagaataaga ttcattcaac
     7381 gcacctgatt gtcctaaata ttctaaaccg gcagtatgtt ccgtcacacc atgtgtcatt
     7441 tcatgaccaa ctacatctaa agaggaagca tacgtttttc ctgttggtgt acttgtttca
     7501 ccataacgca ttgcttttcc atcccaaaaa gcattgtcat aagcatctgg atgttgttca
     7561 tcaacaactg acaagatggg catccctcga tcatcaatac tgtgtcgttg gaaatgatct
     7621 tcataatatg tttttacaaa ttttccatgc gtataggcgt caacagcatt ctgatttaat
     7681 gtcgctagtg atggcgcttg aataatagaa taattatttt tgccatcaac tactgcatgg
     7741 taaacccctg tgttatccgt tccgtgtaaa gcaattcccg tattgctttt ttcaactggt
     7801 acattaaacg ttacttgaaa agagtttttt aacgttactt cactaccgac atgttccgtt
     7861 aaatcttgtt tttccataat tgttccatct gtagcattta ctttataaac caatgatatg
     7921 gggtcaccag ttggtgaaga agttactctg actttataaa ataattgccc ttcattattt
     7981 acacgtttgt caatagctaa aaagacaaga tctttatttt ccggattatc agaagcaata
     8041 atttttttaa ctccttcttc accgattgca tcaaccgatt gaattttcac aacttgatca
     8101 actggatttc ctgtaacact tgttactgca ccgtttttat ctaaatgaat aatcacatta
     8161 tctgaatcaa cagtaacgcc ttccgtttgc ttttctaact taagatgctt catcccccac
     8221 gcgtcttctt ttcgatctac aaccgtatat tgttcagcag ctgcctcaga aattgcctcc
     8281 ttttccaact tcgataaaac tgttgaaact tcactttctg aataaacttg ttcttctgcg
     8341 gctacaaaaa gtgaagaaaa tagacatgaa cttccaacaa agatgcctgt acctaaaatg
     8401 tataaaattt tatttccctt catcaaacaa ttaactcctt ccccagtttc cttttatttc
     8461 ttttcccaac aaaagtattg ctttatcctc cctaattttt cccgttaaac gaccgcccct
     8521 ttccctgcgt aaatatttgt tgctattttc attatagaca actacctaac cggcactatc
     8581 taaattcctt aatcagccga catttttccc tgtattgccc ctcatatctc aataccatag
     8641 caaaaaagtt gttaacaaat tcattcgtta acaacttttt tactgttatg atttttttca
     8701 ataattaaat gttgagtgac cgatccttct ttgaaggacg tttgtaaatc aaattgatct
     8761 gaaaaatcaa caatttcttt tacatataac aatccccaac cgtgctcttc tgggttactt
     8821 ttagaactaa atttggtttt cttcataacc ttaaaatcaa attcggcttg gacactattt
     8881 gttatcacaa actcttgtgt ttcatcttta ttgaaaattg agatagcgaa cattttcaat
     8941 tctgattttg cactgttttc aatcgcgtta tctaatagaa tagccaacaa acgaatcaca
     9001 accactaagt cgattgatgt atcaagaata aatacttctg gcacatcaac aatcactttt
     9061 agttttgctt gctttgcggt attaattttc atacttatga gtgccttaag ttccatattt
     9121 ttcaaacgat ttaacttcat aagttcaaat tcttcattat caataatttt tttggttggt
     9181 gcaatcgttt cttcataaat tcttttgatt tccagaatat cttcgtatga aatagcaatt
     9241 tgtaaactat acaataaatt tttataatca tgacgaaaca tcgctagctc ttcgttaatg
     9301 gattctattt tttcagtata aatcgttaaa ttattcaata attgagtgtt gatacgttgt
     9361 tcttccaaat aagcttcaat gactaagcca atcagaatta aaaataaacc tacacttatt
     9421 aataactcca atttagaact atttaccgtg acaaatcctc gcataaagtc aggtgaaatc
     9481 tctggcgaaa tcaaaaacac aaaaagtaac aacagaatca acaataaaag taaacaacca
     9541 aaaaaaattc ggctattttc taacacacga tttaatctta aaaaaacact attgggaatt
     9601 tttaaagcaa tccagatatt tataagcaac agaagaacat ttgaggcaat ccataataat
     9661 ttattttgat aaatctgttg aaaaaaaaca cttaaaatcg tttgctccgt gtaattgata
     9721 aacgaaacca taagcaatgt aaaaacggat aaaaatatcg ctttaaaaat tgagaacctc
     9781 tttttgtgaa tgagtaaaaa tcctgtaaaa acaacgactg tcagcgcata aatcaaccaa
     9841 gagtagccca acattaatgc cgcaaacgaa caaagattaa tgacaataag caatgaaagt
     9901 aacggtattt tacattcaat cttgatcaat gttactcgta aaaagacaaa aacgataaag
     9961 ctagatgcca gcaaaacgtt agtagctaat aacgacaaaa tcatttttca atattctttt
    10021 taggtttttc agtttgtccc atccattgtc caaatatatt gggcgaattt tgatttaatc
    10081 taggcttagt tcccacacca tcactgactt ttgcaactct tttttcaata acatttttaa
    10141 ttattttttt accaaatttc ataaaaacac ttccttcaat taaatttttt gttacaggga
    10201 gtatcatcag accttggatg acgagaccgt agagtattac tgaagcaatc ttttctttga
    10261 caaacagact acataaaata agtaaacaca cccttataag gctttgcttt ttgagcacgt
    10321 ttaaatcaga tggctgaaca ggttcaattg ctgttccttg tggcgcatac caataaaata
    10381 atacgactaa gaggcaaatc aatagaattt tataaataaa cggtaagctc acagaagaat
    10441 ttttcaatac aaatggtatg gcgacataga taacaatgct ttctagacga cagagccatt
    10501 tagtttttgc atgccagcca cctgcgacag gccgtaaaaa aatcagtacg acgtatacaa
    10561 taaaggtttc gcttaacgtc ccaaataaaa ctgataaaat cagaatcatc agaaaattaa
    10621 ataagccaga aaaataaacg gttagataat actttgtcca ttgtgttttt cctgattgat
    10681 cttcctgatc catatccata atatttttta gaatccaatc gattagcata tcgccctcct
    10741 cttcaagtat tgcactaaat tataacgaat atagcgacta atagatagat aattccttaa
    10801 ttagtcatta tccctcccta agtaagaaat agtgccttga cctgagtata atatcttctt
    10861 gagactggta cttccgttcc gtctctcata gttaaaagat gcgttttata atttaatttt
    10921 gacgcatatt ttttattgat gatgattgat tgatggactc taatcaaatc tggatgcagt
    10981 tgttcaattt ttgataacgt tccataaaat tctcttttaa aatttttacc aaccattgac
    11041 acacgatgat cgtattccgt ttgaaagaaa taaatatcat taatatccat ttttatagaa
    11101 gacgctttta agttaatgat aatatattca ctttttgtct ttatattttc tttttgttga
    11161 atatatgtat cgacacatgt ttcgacctct ttttggaaat ctagaaaatc cacgttcttt
    11221 tgaataaatt gaagagctga aaccatatat ttgtaggaag ttaaaactaa ttctgtgtga
    11281 gtagaaataa atacaatcag actttcactg tctgtttttc tgatttcttt agcaacctca
    11341 aatcctgcct ggatatgatt gttcatttga atatctagga aaaagatatt tagttgggcg
    11401 tcctggtgtg ccacttgtaa taattcatct gaacgtgaaa ataaatggat tgtttcatta
    11461 taaatatttc ttgaatgtag gatatttgtt aacaatgctt ctaacgcttt tgcttgaata
    11521 atttggtctt ctaatatata aatagccatt tgttcactca tccctttctc aagaagatat
    11581 aaaattacac aacaaaagac aaatgattat aatgttcaat caatttatac accaataaaa
    11641 gagataaaaa aattgattgt ctgttagtat attcacataa atttagtata gcgatttcag
    11701 tagtttcctg caaacaaaaa cataaaataa caaagtatca agaaaaagta ctttggacta
    11761 ttcatctttt gctaacgaat catgaggact attgtctctt cgttgatgaa aaacaaagaa
    11821 tcaaccgcca aactgttctt gtcattttat acatgacaaa atcaattcat tggtcgattc
    11881 tggttatttt aatttgttag ttcacacatg gatcattgtg ctaatttttt acttaaacat
    11941 ccacagttct tcctgttgaa tgagtcgatt aagcgccaat gtaggtcacg ccactactat
    12001 aagcaattac attaggtaat acacgatacg aaatcgtttg atcattgatg acattgcttt
    12061 cagaaataat gattccatca gaagtgaccg cttcaacaaa agcaacatgt ccatattgat
    12121 tatctgcgcc agcaactcct ggttggaaac taatcgcacg accagcttta ggcaatgaac
    12181 taacttgata gcctaatgca cgtccttttc ttccccagtc actgccattt cccataaatt
    12241 catccacaga agtgcctaac tgtttgaaac gattatagac ataccacgta cactggccca
    12301 cgggatagct accacttcga ttataattga tcccatcata gactggaaaa tgcatttgtt
    12361 gctgttccgc ttccgacagc ttatttttag attggataat caaaccgtcc actgtttttg
    12421 gtaaatcaaa ttgcgttaag ttataaaccg caattaacga atttaattta ttatcgtatt
    12481 gcttgtctgt tgcatattta cctgttaaaa aacgcgttgc ttgaaggtag tttttggctt
    12541 ccgaacgcca cgctggttta taaaaatctt gatttccgtc aatcccttgt cggagtaatt
    12601 gaacataatc ttgcaatgag tcattatagc caccgtaatc gcgaaaccca gcggaaattg
    12661 tataaagttg gcccctctga tcagcctctt ttgttgaaaa gctaacacta tttccttgga
    12721 acgaaccttt cacgccaaag agattataat aaggttcttt ggctagttga ctttcgccag
    12781 agccactttc taaaatagcc tgagctatca tcactgatgc aaaaacatcg tatttttcgc
    12841 caagttctct ggctgtctca ccaatttttt taataaacgc tgtggtttgt tctgacttct
    12901 gcatttcaaa tgacgcacca tatgttgcta aaacttcttt gccatactcc gttaatgtga
    12961 actccttaaa aggattagtc actaccaagc gtatttcctc ttttgagtct ggttgttgtt
    13021 tggcagtata gatattctct aactgagcga ttacttctgg ttcctcctta gaatccgctt
    13081 ccgctgcggc aattaaatat tttccttggc caagataaac accattttga agcgtttttc
    13141 cttcagctgt ttgccaaaaa atcagatcac caggttctgc ctcctctata gaacgaactt
    13201 taccaaatgt catttgttct tgcggtgtac tgccaatgtc tattttaaat aattgttggt
    13261 aaattttttg ggcaaggaaa ctactggtca atgcttgctc cgtcaaatta aattcttttt
    13321 ttgttttccc aacgtgtctt aatgcttcag ccaccacgac tgcccgtttc ttttcgccaa
    13381 aagacgacaa cagtggaagt tcgtagcctt ttaaatttga agaagcgatt tcatctactt
    13441 tgagggacgg atttaacgat acagcctgat tttcttcaac aacgttattg gtggcttcag
    13501 gaacttgaat aggttcttca ggttcttgct tggtcacagg aacttgtggt ggttgcgatt
    13561 gttcaacagg ctgcgtgatt tctgatgata ccgttggctc tgtaggttct ttttttgttt
    13621 cagaactagt atttgtggtt gtcgtttctg tcgttgactg actcgttgat tcggtagtcg
    13681 tatcggtaga actatttgat tcagatgacg aactactagt ggacgttgta gatgtagcaa
    13741 cagttgtttc ggttgtttcg gttgtttcgg ttgtttcggt tgtttcggtt gttttggttg
    13801 tttcaattac tttttcttcc gaagtaattg tcgttttcga ggacagagct gtttgtagcg
    13861 agctacttgg taattctgca gccaacgtaa caactggagc gacttccaaa ctcagaagaa
    13921 cgagaatact tgttaaaaca agtagttgat tcttgatttt ctttcttttc attcgtaaaa
    13981 atcctctcct attacttact tgcgcatttg cacactgttt ttgtacaaat gcgccttgta
    14041 agttaatgat acgctaattt tggtcttttt tatttttgaa gaataatttt cctaaaccaa
    14101 tacttactat caatagaaaa ccactgattg accagattgg cgaaacaact tcatttgctt
    14161 ttggcaaagt gccagaagtg ctggttttcg ttgtcgttcc acctgttgct gtgccctttc
    14221 ctgtgctggt tacgctacca gtcccgctaa ccgtgccatg atttccattg ttgcctgtag
    14281 agttgctact gattggctct tctgccattg cttcaatggc ttgtgctaaa atacttaaac
    14341 atttgtccac agcttcttgc gtcgcacttg ggtcctcaac aagtgctttt gcttgtttaa
    14401 ttgcttcagc taaagcttct tgcgatgctt ttgtaaattg ttttcctgct ttaggtttca
    14461 atgtttctgc tttttgaagc agttctttca actttgtttt atccgtttga aggacttgtt
    14521 cctctaaact atccaaagca gtttttacat ttttttcagc agtatcaatt tgttcttgcc
    14581 gagcattttt atcatctaaa atcccttccg cttggtcaat tgcttcagtt aagcgcgctt
    14641 tagactcgct ggtaaattct tttccggcag atggtttgat tgcttttgct ttggctatca
    14701 ttgcctctaa ggctttttta tctgtaacta acgcttcttc ttgtaaccca tcgatggcac
    14761 tagttaattc agcaaccgct tcatcaatca ttttttgatt agctgctggg ttttccaatg
    14821 tgtttttagc tgctaaaagc gctttttgta aatcttgtaa cgaggaggca gtaaattggt
    14881 gcccttcact tggagtgact tgctgcgctc gtttgacaac ttcttgtaat tggtctttat
    14941 ttacaagggg tacttctttt aattgagcca ttgcagtttc caatgcgttt agagccgctg
    15001 agacttcttc ttttgtcgcc gtttctttcg ctacgattgc ttcagccgct tgaattgctt
    15061 cttgcaattg tttttctgtt tcttttgtaa attggtatcc ttcttccggc tttattcctc
    15121 gggcttgagc gattttctct tttaaggttt ccttatcaac ggcgacgggt tgttctttta
    15181 attgtgcaat tgcttcacgc aatgttttag tcgcttggtc tatttcttct tgggtaactt
    15241 tttcttgagc aaatactttt tgagctgctg ctaatgactc ttcgagaact tttttagttt
    15301 cttcggtgaa ttcttgccca tcacttggtt ttttcgccag cccctcttga attgcttttt
    15361 ctaaatcagc tttattcgct acatcaggca ttttttccaa ctgttctaca gccttttcta
    15421 gattttccac agcagcagaa acgtctgtct gtgtggcttg ctcattggct agaacagcta
    15481 ctgcctcatc taagattggt ttaaagattg cccaagtttc aggcgtatat tctttttcga
    15541 cacgtgcttg atttagcgta attagcgctt gtaataactc tttatctact agctctgtgg
    15601 gcttcactgt tacagaaaaa tctgaaagtg tggcttgttg gaatccttgg cctactaata
    15661 agccttgact catatcaaat tgtaatggct gctcaacagt tgtcgcacgt tttacgcgag
    15721 ttgtttcttt tactttaact ttaaaggtaa ctaagtccat tgaaccattt aacaattgtt
    15781 tatttccttg attactaaga actaaatata gattctccac atcatttgaa tgtttccgat
    15841 atttagaata attttccatt tgttttgtat tattcgtggt tgcaggctga ccgacaagtt
    15901 caaatttgct agaatcaaag gacatttttg tgcttaacgc attaatgttt tttaagtctt
    15961 gacctttaag tgtaaaggtt aatgtatcgc caggaagata agtatccttg ccattttcat
    16021 taaccacagc aagggataaa ccgcccgcaa tttcttcgac atcaggtatc tcaatcccac
    16081 catctaattg tcgtaaaaca taactgatat cataagcatc aattacccca tttttattta
    16141 aatcaccttt ttcaacatag ccattaaagt cactgtccac cgattctaag cctgtatagt
    16201 ttcgataact cattgcatca ttttcatcaa tcgttccatc gttagtaatg tctccatgta
    16261 agattccttc tgttcctggt tgtttgtaaa agagcatctc acgaccagag ccaaaattac
    16321 ccaccgattt taataccttc atttcaacaa aacgatacgc ttggtccttc gtttcaatgg
    16381 tttttgtcaa agcatcttgc ttccagttaa taggttctga aaactctgtc caattagctc
    16441 catctttcga cgtccgatac tggagctgca aaatatttcc gttgccggca ttgtctcttg
    16501 gcaagtattc aattttatcc atttgatact ccgcaccaag atcaaatttt aaagacaaga
    16561 aatttccatc acttggatta gcgatgccgg ttgaccagtt cgtatgccaa cctgtactca
    16621 aatctttatc tgttaatttc ttcaattctg cgcctggttg ttctggtaaa ttacttgttg
    16681 ctttgacttg attaattgtt tctttatagg gatcatcttg tgtttttcct ttaatcggtt
    16741 cactccattc agagaccccg ttttttccaa cagctcgaac tcgaaaagta tgttcgctta
    16801 aaaaggaaaa tccatcaaat gttgctgtat tcgtttgaat atttccgaaa acagtcccgt
    16861 cacgctctac ttcatagctt gttgcctcag ttacttgatc ccattgaaga gtcaaagaag
    16921 atggcgttgt tttttcttca ttgattgcga catttgccgg aatagttaac tggtcatcga
    16981 ccgttgtatt tcctccgtca accgttcctt tattaatgaa acctttgaca gttatttgaa
    17041 catctttggc ggtcacagat tgtttcgcta gttttactga taacgctgat tggttcagtt
    17101 tttctcctga agcttcagaa agatactgat tcacttggaa ttctttatca aaataatata
    17161 gatttgtgcc agctaaaaat tcttctttat tagctgcttg tttcaaggtt acttctgttc
    17221 cccccaccat tgctgttacg ctttcgggcg cttctgaagc tagcaaatca agcgtggtgc
    17281 ttcgttcatc aacaaagcca ctataacttc cttttgtcgg ttcgatagta acggtcagat
    17341 cccctttttc gtttgattta gggccttgac tatttatttt agtcgtagct gattgcccag
    17401 cttcataact agttgaaatt ccgtcatctt catacatatt aaaagaagtg gcgccatttg
    17461 gataaatcaa gaaagatcgt tgatcccgtt ggatttcctt tggattgttg tttggattcg
    17521 tcattgggat aatactgcca tctttgacga aaacgggcac cttccacagc ggtgttttta
    17581 ctccatttaa gacacggcca ccttgatatt tttcgcctgt aaataagtca acccagactt
    17641 gtttttcatc tggtaagtag attccatcac ggattgaatt tcctgcctca tcttgattgc
    17701 cattataaat tggggcaacg agtaaattgg gtccccacat atattggtat tgagaatctt
    17761 ttgtatatgc tgttccttca ttaggaaatt ctaaagccat ggcccgtacc attggtagtc
    17821 catcaactga ttccttggca atactgtagt tataaggcat catcattgat tttaatttta
    17881 gatacgctcg gttcaaatct gtggcttctt gatcaaaagc aaatggcgtt tttggattgg
    17941 agccccagcc gtccatattt agctgcactg gtgtaaaagt tttccattgg aaatcacgta
    18001 tgttgacctc tttatttttt ccaccaaaaa ttccatccat atctgaaccg acatttggtt
    18061 gtccagacaa cgaagtcccg atatatgttg ggatatggaa gcgaatatat tcccattgac
    18121 cacccgtttg atcgcctgtc caaataccag cgtgccgttg ggttccggcc caaccatcta
    18181 atgaaacaat catcggacga actgcgccgt ctgtttcttt tacaaaaaca ttcgcggcat
    18241 cttctacacc atttaagcca aaggaatacc catacccgac ccacgccacg tcagttttga
    18301 gagctttaac accagcgaca ctaacttctt tcgcaatatc ccgttctcct tttttaggat
    18361 ttttaggatc cgctggatgg agattagatt gggtccacag ccccacttca acaccattgg
    18421 cttgagcata atcagtaaat tcttttaagt tttgtacatc gccgtccaat gaatctgttt
    18481 ggccatagcc agcaccgtag ccgtcatttg gcaagaacca ccctaacggc atatcgtttt
    18541 tcttatatcg atcaataact gcgcgtgcag aaaattgata attctctttt tctccattta
    18601 aagattctaa cgtgccattt aagtttccta aatcgcctgg ttggtattct ttgtaaaaat
    18661 taccatcttc aaacttaact gctcccgcgg ttccttctgc tactttcacc caatagtccc
    18721 gattatacgc gtttaaatgt gcttcataga acccgtattc tggcatcaaa gctggtttcc
    18781 cagttaattc atagtaatct tttaaaatac ctgctgagga atcattaaag aaataaaaag
    18841 catcaaaatc tgttccttcg tgcgttgttg tggttgtttg tggatcatga cttccaaaat
    18901 catagttgcc aggtttccat gtatttcgaa caacgccata ccctgcagtt gaccaataaa
    18961 atgggttagg cgatgctact ccgccatcta cccaattgtt cgtattgaca atctggatag
    19021 ctgttccttt atgcgtaaat cgaccatttt gagttcctcc gccaaaataa ttttcttgac
    19081 tactttgttt taacgtttgt gtagctttat catttttaaa ggatagcgga gcggtttctt
    19141 ctaaaatgac ttgatccttt ttcttatcta gaactttcat taaagcgctt tctttttcga
    19201 acataatttt taaaccatta ttttctagaa taaattggtt tcctgaatct gtgacgttcg
    19261 tttgctcaaa ggcttgtgtg ccatagtcgg ccattgtttt agccgtaatt ttcgcaacat
    19321 gctttggatc atttggcgtt ggatattctt caaatttacc agttggatct agatgatagc
    19381 gaaaaagatg gtcgtttaaa atactaattt gtgctttttt gccagaagca tacgtaatga
    19441 caaagttttt ctccgctttt tcaatcgaca caattgctcc gactgacgtt gctgaatcag
    19501 ctacgtcttc ttttgctgtt tgctcttgcg catcaaccac ttgagaatcc atcgtaacaa
    19561 acgaatggaa cgccaataca gacgaactca ctaataaaaa tgtcaaccag tgctttttac
    19621 cgtaaatttt tttaccctga tactctttgt tcattttttc ctccttaaaa ttgctcccca
    19681 tagaatagtt ccgacacaat tactcgcaaa atccaaaacc caacaaagtt ttatccagat
    19741 gaaaagtcat aacgattttc ttagtttact gagactgtta gaaagtcagt ttcagtaacc
    19801 ccttcctaaa cacatccttt caataaaata ttaaatgaaa acaatctatt ttcatcttat
    19861 ataaaaattt tagtacagaa tcacaatttc tctatgaatt cgttttcatt tgttgactac
    19921 caatttttca cttttttaat aacataatta tcgtatgatg gtttttttgt gtttgtcaac
    19981 ggttaattat agcagccacc attaacgagt attaaccctt gataaacctg ttaattcact
    20041 tataatgatg attttattgc gaaaatatct acatcagaaa aagaaagact tttttcaaaa
    20101 atctaatacc tgttactcaa ctttagtcta tttaaaaata atcaacaccc tcctgctcag
    20161 caagagggtt tttaataatt gtttttagtg attgatttga cttgctggtg tgaaaagcaa
    20221 actaatgatg atgccaacta tcagtaccgc tagcaatata aaaattaact ttccaaaccg
    20281 gtgataggga ttgatcgaaa gcccaatccc ataaactttc ggaacccaca aaggaggcgt
    20341 tggattatct ttttgatctt tataggctat ctttaaaatc tgccacgcaa aatacaacaa
    20401 tggtaaaacc ataattaccc caactatacc tagccatttt gctaataagt cactagtatg
    20461 caaaagactg attgtcagaa ttggtacaaa caaaagaata aagctaattg tggctcccac
    20521 ttgtgcttgt ttaagcagtc gtctctcacg gagagtaaga ttcgcaaaca ttccatccat
    20581 cctttcatgg ttttcagatt aaatcaacga atcactaaaa aactaaatta actgcgaaaa
    20641 tcaatgacca tgcgcccgcg aatttttcct tgttccattt cttcaaaaat agcattaatt
    20701 tcttctggtt gacgtgtttg acaaacagg
//
