LOCUS       NZ_CP060140            23318 bp    DNA     circular CON 28-MAR-2024
DEFINITION  Nodularia sphaerocarpa UHCC 0038 chromosome, complete genome.
ACCESSION   NZ_CP060140
VERSION     NZ_CP060140.1
KEYWORDS    RefSeq.
SOURCE      Nodularia sphaerocarpa UHCC 0038
  ORGANISM  Nodularia sphaerocarpa UHCC 0038
            Bacteria; Cyanobacteriota; Cyanophyceae; Nostocales;
            Aphanizomenonaceae; Nodularia.
REFERENCE   1  (bases 1 to 5451672)
  AUTHORS   Ahmed,M.N., Wahlsten,M., Jokela,J., Nees,M., Stenman,U.H.,
            Alvarenga,D.O., Sivonen,K., Poso,A., Permi,P., Koistinen,H. and
            Fewer,D.P.
  TITLE     Suomilide, a new member of the aeruginosin family
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5451672)
  AUTHORS   Ahmed,M.N., Wahlsten,M., Jokela,J., Nees,M., Stenman,U.H.,
            Alvarenga,D.O., Sivonen,K., Poso,A., Permi,P., Koistinen,H. and
            Fewer,D.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-AUG-2020) Department of Food and Environmental
            Sciences, University of Helsinki, Viikinkaari 9, Helsinki FIN-00014,
            Finland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 3; SPAdes v. 3.11.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 77.0x
            Sequencing Technology             :: PacBio RSII; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022376295.1-RS_2024_03_28
            Annotation Date                   :: 03/28/2024 07:15:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,496
            Genes (coding)                    :: 4,450
            CDSs (with protein)               :: 4,450
            Genes (RNA)                       :: 60
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 11 of 46
            Pseudo Genes (incomplete)         :: 34 of 46
            Pseudo Genes (internal stop)      :: 13 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 12:16:42
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1927860
            Orig. end                         :: 1951178
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP060140.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This genome has a base modification file available.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..23318
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="lanthipeptide-class-ii"
                     /region_number="9"
                     /rules="cds(LANC_like and DUF4135)"
                     /tool="antismash"
     cand_cluster    1..23318
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="cds(LANC_like and DUF4135)"
                     /kind="single"
                     /product="lanthipeptide-class-ii"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..23318
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:13318](-)"
                     /cutoff="20000"
                     /detection_rule="cds(LANC_like and DUF4135)"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-ii"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..13318)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(LANC_like and DUF4135)"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-ii"
                     /protocluster_number="1"
     misc_feature    complement(445..458)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: medium, score: 21.16"
                     /tool="antismash"
     misc_feature    complement(652..665)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 20.35"
                     /tool="antismash"
     misc_feature    1259..1274
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 23.25"
                     /tool="antismash"
     gene            complement(2222..5500)
                     /locus_tag="BDGGKGIB_RS07725"
                     /old_locus_tag="BDGGKGIB_01571"
     CDS             complement(2222..5500)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015137987.1"
                     /locus_tag="BDGGKGIB_RS07725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BDGGKGIB_01571"
                     /product="S-layer family protein"
                     /protein_id="WP_239731049.1"
                     /transl_table=11
                     /translation="MALSWSNCYWRFKVASFVAMCAAYTASGDFAKAQITPDSSLGAES
                     SMLTPNVSIDGLPADRIDGGAIRGTSLFHSFREFNIGDGQRVFFANPGGIEHIFSRVTE
                     TNPSTILGTLGVNGGANLFLLNPNGIIFGPNARLELQGSFLASTAQSIKFTDGIEFSTN
                     NPSAPPLLTLNIPIGLQYGQDAGRIIVQGQGNELRINQETGEVLEVNRTGGLAVEPGQT
                     LALAGGEVLLEGGSLSAESGTVEVWSVQGEGLLGLTPTNPGWELSNQGVQNFQEIRLSQ
                     AAAIDTSGSEGGNIQIQGRRVALQDGSFILSLTRGEGSGGTISIRASESIEASGNAPNG
                     ESSLFLSETTGSGKAGDVEIYTEKLILQNGAQVSSSTFNQGSGGKIIVNASELVEATGI
                     AETGKFASGLFSVARQNTGEGGRVEVTTKRLVLRDGGQVSATTFSQGRGGNVLVNASDS
                     VEAIGIASIGSSGLFAASVSNGEGGTVEINTEKLILEDGGQVSAATFNQGTGGNILVNA
                     SKSVEVIGIAQNGNVASTLAAQTRGTGNGGTVEINTERLILQDGGQVSATTFNQGSGGN
                     ILVNASKSVEAIGQNGSFVSGLLAQTRGIGNGGTVEIKTQKLILQDGGRVTATTFNQGR
                     GGNILVNARESVTAIGFGSGLRAQTQGAGDGGTVTVNTGRFLASKGAQTLAAVFQNSKG
                     QGGNINIEADEIQLTGTSDNGDPTIVFATTRGEGDGGEITINTARLIVQEGAQLGAGTF
                     SSGEGGNVLIKASDSVELVGTVPQVPNRPFFRDQSGEQFPSGLFTGSQGIGTAGNLTIE
                     TDRLTLRDGAEIAVNNQGGGDAGNLEVLARNLLLDNKGVISATTTLGQGGNIILQIEEL
                     LLLRRNSQISTTAGIDLAGGDGGNISIDSGFIVAIPDENSDITANAFQGRGGNININAR
                     SIFNLEQRPSIPLNNTNDIDASSQFGLTGTVTVNTPDLDPSRGLLQLPENFTDVSQQIV
                     SSCNPGSSARRSSFTVTGRGGIARSPIELFQGEVSTARWITLDTINQHQISHLIHESLA
                     SSTPQIVEAQGWIFDNNGNVSLVAQLPNITSGGLSVSSGVCPVD"
     gene            complement(5528..5761)
                     /locus_tag="BDGGKGIB_RS07730"
                     /old_locus_tag="BDGGKGIB_01572"
     CDS             complement(5528..5761)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BDGGKGIB_RS07730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="BDGGKGIB_01572"
                     /product="hypothetical protein"
                     /protein_id="WP_239731050.1"
                     /transl_table=11
                     /translation="MKDKHKNLKDIDLFTKLTLSQQEQINGAIGQFTLPQLPPLPNGSS
                     TPLSPLPPLPPPEPEDDWYIIFLGRYLNFVAR"
     misc_feature    5847..5858
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: strong, score: 19.12"
                     /tool="antismash"
     misc_feature    5977..5993
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 23.59"
                     /tool="antismash"
     misc_feature    complement(5983..5992)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: strong, score: 18.31"
                     /tool="antismash"
     gene            complement(6059..7567)
                     /locus_tag="BDGGKGIB_RS07735"
                     /old_locus_tag="BDGGKGIB_01573"
     CDS             complement(6059..7567)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1029: RND family
                     efflux transporter MFP subunit"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015140178.1"
                     /locus_tag="BDGGKGIB_RS07735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BDGGKGIB_01573"
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="WP_239731051.1"
                     /transl_table=11
                     /translation="MVNHINADSLPIVESDEFLPPIGNWARLGSLVIVAGVGIAIALAS
                     IIEYKVTVKGQGSIRPTGELRIVQAATEGQVTKVFVKENQPVKKGDLLATIDDSSLQTQ
                     KSQLQNNIQQTQLQLLQIDAQVTALNRQIQAETEGKKRAVTSAEAALNRAQRNYRDQQI
                     NVKSEVEEAEANLKRAQNELTQAQVELRSARANLQSTEAAVSAAQAKRDRYQKVVQAGA
                     LSLNQLEEVQLDVAQQQHAVEVRQATVEAQQQIIAQQQQAVAAMRAKLQRVQTALNPID
                     AEVAIAQENIAQESASGQATLAILNREWEALFQQRIQIKNQIQRDTRELQQVESNLSQT
                     KITVTEDGIISQLNLRNSGQSVRVGEEIAQIFPSNAPLVIKAAVSPDDVSKLAKDQKVQ
                     MRVSACPYPDYGTLKGFVSQISEDTVKSPGNQLGSTVANASSPMQNSTSSFYEVTIVPE
                     SLFLGKTNHECTIQPGMQGRVDIISRSETVLKFLLRKARLIADW"
     gene            complement(7734..9962)
                     /locus_tag="BDGGKGIB_RS07740"
                     /old_locus_tag="BDGGKGIB_01574"
     CDS             complement(7734..9962)
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017747626.1"
                     /locus_tag="BDGGKGIB_RS07740"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BDGGKGIB_01574"
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="WP_239731052.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 3.6e-35, bitscore:
                     118.7, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKFQLIQQHSEEDCGAASLATIAKHYGKILSMSRCREAVGTRQEG
                     STLLGLKQGAEALGFNARAVKVTLEVFNEKTISLPAIIHWKGYHWVVLYGKQGNKYVIA
                     DPGAGIRYLEKKWLLEGWTNGVMLLLEPDPVRFVTQEDQPEKIGGVGRFLRRVWPYRAV
                     IAQTLLLNLVLGLLSLTSPFLLQILTDDVLVRGDRQLLTSVVIAVVVMHLVSSSLQLAQ
                     SNMVAHFAQRLQLGLIFEFGRQILRLPLNYYESRRSGEIVSRLEDIQQINQLISQAVVS
                     LPSQLFIALVSLSLMVFYSIKLTAVAGAIAILMTLSTILSLPTLQQKIRSVLVLSSENQ
                     GVLVETFKGAITLKTTAAAPQFWEEFQSRFSRLANLSFRTIQIGILNGIFSNLVSSIGS
                     IALLWFGSTLVIQQELSIGMLLAFNSMNGNFISFIKTTIDFVDEYTRAKTATQRLTEVI
                     DATPETLDNSRKPWVKIQSNADITCTNLNFHHPGRVELLQDFSLTIPGGKVIALIGKSG
                     CGKSTLAKLIAGLYPLQSGNIRFGIYNLQDLALDCFRQQVVLVPQEAHFWSRSIMDNFR
                     LGSPDITLEQIVQACQIAGADEFISNLPDKYQTVLGEFGANLSGGQKQRLAIARGIVNN
                     PAVLILDESTGALDPVSEAQVLNQLLTHRQGKTTILISHRPKVIQQADWIVMLEKGQLK
                     IQGSPEILSQQVGEHLDFLDGVDISRVNGLMKNPANFHRNGSSATSIC"
     gene            complement(10001..13318)
                     /locus_tag="BDGGKGIB_RS07745"
                     /old_locus_tag="BDGGKGIB_01575"
     CDS             complement(10001..13318)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: LANC_like"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: DUF4135"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1070: lanthionine synthetase C family protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016860136.1"
                     /locus_tag="BDGGKGIB_RS07745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BDGGKGIB_01575"
                     /product="type 2 lanthipeptide synthetase LanM family
                     protein"
                     /protein_id="WP_239731053.1"
                     /sec_met_domain="DUF4135 (E-value: 9.9e-113, bitscore:
                     375.1, seeds: 79, tool: rule-based-clusters)"
                     /sec_met_domain="LANC_like (E-value: 1.8e-74, bitscore:
                     248.7, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTLSQSTSNSTIKEDPFQKLPAAPSSTQSEAWYHALTLKERIALC
                     ADTSKTPKKNIDEKQAERLLERWRSQFSTGAFFDQRLALDGINENEFLELLGERINAVQ
                     DRCATPPAWLEGLTQAFARPMSFDAVALLQQWHNQPEVGFLYLIEPLLRQAIDDFRQGI
                     QKLGETHSSLPFDPDTIEAIVLVNWTQELLQLLKRTLVLELNVARLQGLLQGDTPQERF
                     ENFIQQLRHSDVAFALLEEYPVLARQLLIHIHHLSSFSLEFLTHLCQDWHTLQTTLGSN
                     ATSDKLLQLEGNLGDKHRGGRSVLIATFTNGWKVVYKPKSLAIDIHFQELLSWLNQRGD
                     HPTLRTVKVINRGSYGWVEFVKAEDCDTNIEIERFYERQGAYLAILYGLSATDFHFENL
                     IAVGEHPILLDLEALFHPPRERRDENNTEQLIVNKILQSVLKVGLLPMRLWFNSESEGL
                     DISGLGATAGQLSPDPLPYWEGAGTDQMQLKRSRLPLKEGDHRPKLQGKAVRLLDYISS
                     LINGFTAMYGLLLKHRDHLLSDSGPLAAFGEDEVRVIVRPTRFYGMLLYESFHPDVLRN
                     ALNRDRLWDKLWSEVKNDFDLVQIILAEREDLEQGDIPAFFTYPGSPDLWSSSGKRIAN
                     FFDESTMSSPARRFLQLSDNDLAQQTWYIRASLATTVTGDELKHASVSRPTISHSSNTV
                     ADQSQFLAAALAVGDRLESLAIQGGGDATWVGLALVDQRNWMLASSGVDLYTGLPGIAL
                     FLAYLGAVTHQERYTVLAQAAMKTLRHEVKQVKSAISSIGAFTGWGGLIYTLTHLGVLW
                     EQPELIAEAEETAALFPSLIEFDQQFDVIGGAAGCIVSLINLHHHAPSLLTLQTAIQCG
                     DWLLAHAQPMEGGLAWVANGFSKSPLSGFSHGAAGIAWTLFKLARFTGEERFETAARQA
                     IEFERTLFCQEEGNWFDRREAEIPEQGTEKVSPNLITAWCNGAPGIGLARLHCLSYLDD
                     SQIRAEINTALKTTLAHGFGGNHSLCHGDLGNLEFLLQASLILDQPQWKTEVDRLGSVI
                     LDSIDQQGWLCGVPLGVETPGLMTGLAGIGYELLRLAAPEFVPSVLVMEPPKVNSQV"
     misc_feature    complement(13306..13318)
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 19.27"
                     /tool="antismash"
     gene            complement(13446..13709)
                     /locus_tag="BDGGKGIB_RS07750"
                     /old_locus_tag="BDGGKGIB_01576"
     CDS             complement(13446..13709)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016878212.1"
                     /locus_tag="BDGGKGIB_RS07750"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BDGGKGIB_01576"
                     /product="mersacidin/lichenicidin family type 2
                     lantibiotic"
                     /protein_id="WP_239731054.1"
                     /transl_table=11
                     /translation="MSNIDIIRAWKDEEYRNSLSEEQRSMLPENPAGILEMADQEIQNL
                     VGGEARYASQPVQSAYLPSWLTNCKNISNAMSVTSTCCCFSR"
     gene            14020..14172
                     /locus_tag="BDGGKGIB_RS07755"
     CDS             14020..14172
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BDGGKGIB_RS07755"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_239731055.1"
                     /transl_table=11
                     /translation="MLSTYPNTTIGSANNRNVQWLYPNGRLLQTVATKWASVSPSSRRN
                     FNESR"
     misc_feature    14039..14055
                     /note="TFBS match to RicR, Copper-responsive regulator,
                     confidence: weak, score: 21.62"
                     /tool="antismash"
     gene            14324..14452
                     /locus_tag="BDGGKGIB_RS22735"
     CDS             14324..14452
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BDGGKGIB_RS22735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_272075674.1"
                     /transl_table=11
                     /translation="MIDFGDGGFTYFDNCSKVWRSSGVREIMYFLHIWQNTWSPVY"
     gene            14504..15679
                     /locus_tag="BDGGKGIB_RS07760"
                     /old_locus_tag="BDGGKGIB_01578"
     CDS             14504..15679
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_function="GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016877859.1"
                     /locus_tag="BDGGKGIB_RS07760"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BDGGKGIB_01578"
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="WP_239731056.1"
                     /transl_table=11
                     /translation="MQPRQGITEIFSTFVQLDADRFNVWATDSKLQRSIKRCVEAYPQQ
                     KSDNFWVLYWYKIWEIESNPLAVGHISAYLQEVCYWVSRKIAVNFISQFSIADCFQMAI
                     SCIYKILKNFNPEYSTNLKSYAEYAFERFLKDTLRLGKEADICTDWALLHKISRKRLVN
                     SLTNAGFNSQISNNYVLAWECFQELYTSDSKTIRQLGKPDSTRWQAMTQLYNRQSLSQV
                     TPETLEKWLSTCAKAVRDFLYPKFVSVDAFFNGQESGNLLDTLPADLPASLLTEIIAQE
                     ESTTRETQQAQLNQVLMDALAALDIQAQKLLQAYYQQQLTQQQISEQLEIKQYTVSRRL
                     SSIKRSLLTTLTQWSIDSLHISPTPVVVDAISKSLEEWLKNYYSHTHPPLK"
     gene            15694..16851
                     /locus_tag="BDGGKGIB_RS07765"
                     /old_locus_tag="BDGGKGIB_01579"
     CDS             15694..16851
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015137995.1"
                     /locus_tag="BDGGKGIB_RS07765"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BDGGKGIB_01579"
                     /product="DUF1822 family protein"
                     /protein_id="WP_239731057.1"
                     /transl_table=11
                     /translation="MFDAAVAFAVDNDLVLEMVPTSEPLEHNYSTPGGYQRAWLNQICL
                     TTFLDWLQGEITPNGRVHPNRATLPSFWEVVNGTAISFDNSRLVLLPTLAMDVDELRVP
                     QEWVDIPEWVADYYLAVQVNPDEGWMRILGYTTHQQVKTLGVYDPGDRTYSLESDHLIP
                     DLNVLWVTRQLYPQEIRRANVAPLTPLPQTQAANLLERLSHADIKFPRLEVPFPLWAAL
                     IAHGGWRQSLYELRQGISQPASIGRWLQDGVSNFSQQLGWEIQQFTALPSGMRSRENQT
                     AILGLSRQILIADNTYELRVFPKGNPEEQIWRFELRNANPENMIPVGLQLKLLTEDLQP
                     FPNNEDTATTPVEQLYVEVMLEPGEGLVWEVTPLPTEYEREILRF"
     CDS             16867..16977
                     /gene="allorf_1944727_1944837"
                     /locus_tag="allorf_1944727_1944837"
                     /protein_id="allorf_1944727_1944837"
                     /tool="antismash"
                     /transl_table=1
                     /translation="MIRKNYFFLNHRDTETQRNTKITNSSSGATLRLCVN"
     CDS_motif       16867..16953
                     /prepeptide="leader"
                     /note="peptide class: lanthipeptide"
                     /note="predicted sequence: MIRKNYFFLNHRDTETQRNTKITNSSSGA"
                     /locus_tag="allorf_1944727_1944837_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       16954..16977
                     /prepeptide="core"
                     /locus_tag="allorf_1944727_1944837_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class II"
                     /score="-5.00"
                     /molecular_weight="800.0"
                     /monoisotopic_mass="799.4"
                     /core_sequence="TLRLCVN"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MIRKNYFFLNHRDTETQRNTKITNSSSGA"
                     /leader_location="[16866:16953](+)"
     gene            16986..19622
                     /locus_tag="BDGGKGIB_RS07770"
                     /old_locus_tag="BDGGKGIB_01580"
     CDS             16986..19622
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1041:
                     transcriptional regulator, SARP family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015137996.1"
                     /locus_tag="BDGGKGIB_RS07770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BDGGKGIB_01580"
                     /product="CHAT domain-containing protein"
                     /protein_id="WP_239731058.1"
                     /transl_table=11
                     /translation="MFFTLKVWEVVKKKEYRKSKLINFCLLAVLSISICIVVTPAFANI
                     SQNLINQDSTSKIAESQILYERGRFADAVQILQQAVEEYQQQGDTLKQAVALSNLSLAY
                     QQLGHWQKAQIAITKSLNLQEFSQKERTNQNLALLAQSLDIQGRLQLLTGKSEASLATW
                     KNTEDIYTKLEDNNSIALTLLYQAQALRNQGFNKQAMEKLSQINKTLASQGDSLAKAAV
                     FLSLGATLENVGELEKSRLALEQSLEISQRLKSPQNIALSLLSLGNNARIQQKNLQAIA
                     LYEKAFEISTSPLTKVQARLNHLNLLIEEKEFAAAQILIPEIQSLLAELPASRPSIYAR
                     INFAHSLGSLNTPKAQIAQLLATSVQQARSLDDIRAEAYALGNLGSLYEKTQQFAEAMK
                     LTEQALILAQTSNAPEIKYLWQWQMGRLLKAQGNFTGAIARSAVPKAIAEYDAAIKTLE
                     SLRADLVAVNQDIQFNFRDNVEPIYRESVALILQNSGEKPNVKTLDKARTRIEALQLAE
                     LDNFFREACLQGERVLLDQLVDKENPNTAILYPIILPEELQVIVKIPQQPLRHHTVKKS
                     QVEVERTLAKLREYILEPDRTEEVQALSQQVYNWLIKPIASDLTTSKVNTLVFVLDGAL
                     RNIPLAALYDGEKYLVEKYAVALSLGLGLLAPRPLAQTPVKVLAAGLVQPPPGFPTFPP
                     LPGIKLEFDLIAQTGASTKQLLDQDFTSNTLENNVNTVPFNVLHLATHGQFSSRPEETF
                     ILANDGSINVLQFDNLLRSQIDNSRQILELLVLSACQTATGDNRATLGLAGAAIKAGAR
                     STIASLWHINDQSTAILIGEFYNELVNSKVTKAEALRRAQVKLLTEYPNYSRPGYWAAY
                     VLVGNWF"
     misc_feature    complement(22673..22689)
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: medium, score: 23.45"
                     /tool="antismash"
ORIGIN
        1 caccatctgt gcttgtaaaa agactccggc tatttcctgt gatttccctt gagcagaagc
       61 atagggagtc aatgaataat cacgaaagct ttcggctgtt gtgccaatta aaactatctt
      121 gtctttgact gcatcggctt gtagttgacc tgctaatgct tgttgtaaag taatgcgggg
      181 tgcgatcgct tctaagttag gaaaagagcg ataattgagt aaaatttggt gtcccaacgc
      241 gtcaatacct tggtaacctc cggtatgagc ctcaattggt tttaaggtta gctgacctaa
      301 ttgccatgca ccatcagggc taaattgtaa ctgaattcct tcagcatata aataacgtaa
      361 tgtcaactgt acatttaagg cataaggtac agtacaggga gatgagggag gaggagttaa
      421 cgccaacaga tgccgtcgca cgacatgatc ggcatcaatg acaatatcac tgaagccgag
      481 agcatcagga gcaacttccg gtggtggttt gatacctggt ttgccatttt gggggttgct
      541 aacttgacat actgccacaa agcgatcgct gtttctcatg cgttctgcca acctgggata
      601 agctggctgt acagcataat ctctgtatat atccaaacca atcacccgtg gttgatatgc
      661 ctccaatttt gccaacaact gatccagcgc ctcatctgat aaagaccccc gtctatcagg
      721 agattgggct tgcacatctt cttcagtaat ttcaattatc agtatacgag aatctggctt
      781 ttcctgggga cgcagacgca gcaagtggtc aaacgccatt aactcccatg attgcagtaa
      841 accaaaatac cgtactccca ttaacaatgg tgttataaat atactggcaa gcatcaccgc
      901 agataaagct tgtcgtcggt ttagaccttt cctagttgag gttttaggta cagaagatat
      961 ttcctgactc ttctggttga cgtgtaattc tcgccataat agaggcatcg ctgctggatt
     1021 ttgataaatt acaggcaacc aagtagcaca aggaaattga gtttctagtc cttgcaaccg
     1081 ttcccgcgct tctttgactg ctatgtataa agattcacca cgagcaaaag cttctaaaaa
     1141 atacttgagg aattcttgcg ccacccgatc aggaactggt tctcgcatga cgataatttg
     1201 gggtatatgc aaatctgcaa actctcgcgc caaacccaaa ccatcacaag aattaaaaat
     1261 tgccaaatgt aaacctcgtt ctactgcttt tcgcaagccg tactttaact gactaattgt
     1321 caaacactct gtttgattaa tataaatttt cccttgatta aggcttccat cagtagcact
     1381 atgtccggca aaaaacaaaa tctgccagtc ttgttcccat agctgatcag tgagttcttt
     1441 acttgatggc tcgactaaaa aggtagtatt tgcatgaggt aattctttta aaagctgact
     1501 atctgtttcg agatcaattc cctgactatt acccaaaatt gctaaaactt tcacctgcaa
     1561 ttgagggtgt gacttgcggc taagttgttc ataactaggc gcactcagag caacttcggc
     1621 tttgggatag cgttctaata aatcccacaa atgccaaggc agtttttgca atctcaggtc
     1681 attggtttgt actagtatgc ggatatggtc tgaaggtagc agtttttcta gcaatttttc
     1741 gcggatagag cgaaaactag tagatgatag ccaggtattt aactggagtt tcaaaacatc
     1801 cgcagccttt tcgcaatctg ccaaagaagg agtttgtggc gatcgcttag gaataccgat
     1861 agggcgacca gaaaatttaa gttgacagta aatagactgc cattgatggt aacaaagggg
     1921 aatatctgaa tctggtggta attcgccagt aatttcgaca attgggcgca cattttcatc
     1981 accaatttgc aacgtgacag gaaaccccgc ctcaaaactg ccagtgccta actttaaaac
     2041 tactaactta cccaacgtca gtaccccaga tatggcgata atatcacttc ccttagtaga
     2101 acttacgcaa aaaaccactc aaaccctcat aactctgtgt cctctgcgcc tggtgcggtt
     2161 ccttccttca taaatcaaga tgtcacacta atatatacga atgcgatcgc agtatatgca
     2221 atcaatcaac cgggcaaaca ccactagaga cagacaaacc accagaggta atatttggga
     2281 gttgagctac cagagataca ttcccattgt tatcgaaaat ccagccttgc gcctcaacta
     2341 tttgaggtgt ggaagaagca agactttcat gaatcagatg actaatttga tgctgattga
     2401 tcgtatctaa cgttatccat cgggctgtag atacttcacc ttggaataat tctatgggac
     2461 tgcgggcaat accgccccgt ccggtgacag tgaaagaact acgcctagcg gaacttccgg
     2521 ggttacaact agagacaatt tgttgcgaca catccgtgaa attttcaggt aattgtagca
     2581 aaccgcgact aggatctaaa tcaggtgtat taactgtgac tgtacctgtt aagccaaact
     2641 gagaactagc atcaatatcg ttggtattat ttagtggtat tgatgggcgt tgttccagat
     2701 taaaaatact ccgagcatta atattgatat tgccacctcg cccttgaaaa gcattggcag
     2761 tgatatcgct attttcatca ggtatggcaa cgataaagcc gctatcaatg gagatattgc
     2821 caccgtctcc accagccaga tcaatgcctg cggtagtcga gatttgactg ttgcggcgta
     2881 ataacaaaag ctcctcgatt tggaggataa tatttccacc ttgacctaat gtagtcgtag
     2941 ctgagatcac ccctttattg tctagcaaca ggttacgagc tagcacttct agattgcctg
     3001 catctcctcc tccctgattg ttcacagcaa tttcggctcc atctcgcagc gttaacctgt
     3061 cggtttcaat tgtcaagttt cccgcagtac cgataccttg agagccagta aacaaaccgc
     3121 taggaaactg ctcacccgac tgatctctaa aaaacggtcg attgggaacc tgtggcactg
     3181 taccaacgag ttcaactgaa tcagaagctt tgatcaaaac atttccacct tcaccgctac
     3241 taaaagtccc agcgcctaat tgcgctcctt cttggacgat taatcgagcc gtgttaattg
     3301 taatttcgcc tccgtcgccc tcaccgcgag tagtagcaaa cacaatggtg ggatcgccat
     3361 tatcagaagt gccagttaat tggatctcgt cagcctcaat attaatgttt cccccttgtc
     3421 ctttactgtt ctgaaaaacg gctgctaaag tttgcgctcc cttgctagct agaaaccttc
     3481 ccgtatttac cgtcacagta ccaccgtccc cagcaccttg ggtctgcgct ctcaaaccgc
     3541 tcccaaaacc aatcgctgtc actgattccc tggcattgac gagtatattg cctccccgac
     3601 cctgattaaa agttgtggca gttacccttc ccccatcttg gagaatcagc ttctgtgtct
     3661 taatctctac tgtgccgccg tttccaatcc ctctggtctg tgctagcaag ccactgacga
     3721 aactgccatt ttgcccaatg gcttccactg actttgaagc attgacaagt atattacctc
     3781 cactaccctg attaaatgtt gtggcgctca cctgtccgcc atcctggaga atcagccttt
     3841 ctgtgttaat ctctactgtg ccgccgtttc cagtccctct ggtctgtgct gctaaggtgc
     3901 tggcaacatt tccattttgg gcaataccaa tcacttccac tgactttgaa gcattgacaa
     3961 gtatattgcc tccagtaccc tgattaaatg tcgcggcgct tacctgtccg ccatcctcaa
     4021 gaatcagctt ttctgtgtta atctctactg tgccaccctc accattacta acactggcag
     4081 caaataagcc gctagaacca attgaggcaa taccaatagc ttccactgaa tccgaagcgt
     4141 tcaccagtac atttcctcca cgcccctgac taaaagttgt ggcgcttacc tggcccccat
     4201 ctcggagtac cagtcgctta gttgttacct ccactctgcc gccctcacca gtattctgac
     4261 gagcaacaga aaataagcca ctggcaaatt ttccagtttc agcgattcct gtggcttcga
     4321 ctaactctga ggcgttgaca ataatctttc ctccactgcc ttggttaaaa gtactagaag
     4381 atacttgcgc cccattttgg agaatcaatt tttctgtgta tatttctaca tcaccagctt
     4441 tgccgctacc tgtggtttca cttaaaaaga gactagattc tccattgggg gcgtttccac
     4501 tggcttcgat agactcagaa gctctgatgc tgatagttcc tccacttccc tcaccccgcg
     4561 tcagggacaa aatgaatgaa ccatcttgta gcgctactcg ccgtccctgg atttgaatat
     4621 tgccaccttc actaccactt gtatcaatag cagcagcttg ggaaaggcgg atttcttgga
     4681 agttctgcac tccttgattg ctcaattccc agccggggtt ggttggggta agacctagta
     4741 aaccctctcc ttgaacgctc caaacttcaa ctgtccctga ttcggcgctc aaacttccgc
     4801 cttccagcag gacttcacca ccagccagag ccaaggtttg accgggttct actgccaaac
     4861 caccagtgcg attgacctct agtacctcac cagtctcctg attaattcta agttcattac
     4921 cttgcccttg tacgataatt ctgcctgcat cttgtccata ctgcaagcca atgggaatat
     4981 tcagcgttag caaaggaggg gcagaggggt tattagtgct aaattctata ccatcagtaa
     5041 atttgatgct ttgggcagta ctggctaaaa aggaaccctg gagttcaagc ctggcattag
     5101 ggccaaaaat aatgccgttg ggatttagta aaaataaatt ggctccaccg ttgacaccaa
     5161 gtgtccccag aatcgttgat gggttagttt cagttactcg actgaaaatg tgttcaattc
     5221 ccccagggtt agcgaaaaag actctttgtc catcgccaat attgaattct cgaaaactgt
     5281 gaaataggct tgtacctctg atagctccgc catcaatgcg atcggcagga agaccgtcta
     5341 tgctaacatt tggagtcagc atagaacttt cggcacctaa ggaggaatca ggggtaatct
     5401 gagctttggc aaaatctccc gatgcagtgt atgctgcaca cattgctacg aaactagcta
     5461 ctttaaaccg ccagtaacaa tttgaccagc ttagagccat gatgccatcc tcaaaagtat
     5521 taggacttta ccttgctaca aaatttaagt accttcctaa gaaaattata taccagtcat
     5581 cctctggttc tggtggtggt agtggtggta gtggtgatag tggtgtagag gaaccattag
     5641 ggagaggagg tagctgaggt aaagtaaatt ggccaatcgc cccattgatt tgttcttgtt
     5701 gactgagagt cagttttgtg aacaagtcga tatctttcag gtttttgtgt ttgtctttca
     5761 tctttacctt tttttgtttc ttcagtgttt tactagcaga tgcagtttgc gaaacaatca
     5821 gcctgttata gcaatgacga atttgtgaaa acgcttacgt ttaattattc aactttaagt
     5881 tgaaactttt acggaatttt taatttagat taaaagagtt aaatttatat actattaaac
     5941 cacaaataac cgtattttta ctcaatgttt ttgatctctt tataaaaaaa cataggtagt
     6001 taaaatttca gagccttact caggtttttg agcattgatg cgatcgcaaa cacaataatt
     6061 accagtctgc aattaaccga gcttttctga gcaggaattt caggactgtt tctgatcgcg
     6121 aaatgatatc aactcttccc tgcattcccg gttgaatagt acactcatgg ttggtcttac
     6181 ctaagaacaa gctttctggg actattgtta cctcgtaaaa agaactagtg ctattttgca
     6241 tggggctaga agcattggcg actgtagatc caagctgatt tcctggagat ttgaccgtat
     6301 cttcagaaat ttgactaaca aaacctttca gagtaccgta atcgggataa ggacaggcag
     6361 aaacccgcat ctgtactttt tgatcttttg ccaacttact gacatcatca ggggaaacgg
     6421 cggctttgat gactaaagga gcattgctag ggaaaatttg agcaatttcc tcacccacac
     6481 gcaccgattg accagaattt ctcaggttta gttgagagat aatcccatct tcggtaactg
     6541 taattttggt ctgactaagg ttgctttcga cttgttgaag ttccctggtg tcacgctgga
     6601 tctgattttt gatttgaatc ctctgctgaa acaaagcttc ccattctcga ttgaggatgg
     6661 cgagagtggc ttggccgcta gcactttctt gagcaatatt ttcttgtgcg atcgccactt
     6721 cagcatcaat gggattaaga gcagtttgaa ctcgttgcaa tttcgctcgc attgcggcta
     6781 cagcttgttg ttgctgggca ataatttgct gttgcgcctc cacagtagct tgtcgcacct
     6841 caactgcgtg ttgttgttga gccacatcta actgcacttc ttctaattga ttcagagaca
     6901 aagcacctgc ttgaactacc ttttgatagc gatcgcgttt agcttgagca gcactcaccg
     6961 ccgcctcggt tgattggaga ttagcacgag ctgaacgcag ttccacctgt gcttgagtca
     7021 actcattttg cgctcgtttt aaattcgcct cggcttcttc tacttccgat ttgacattaa
     7081 tttgttgatc tcgataattg cgttgggcgc gattaagtgc ggcttcagca gaagtaaccg
     7141 cacgcttttt tccctcggtt tccgcttgaa tttgacgatt taaagctgta acttgggcat
     7201 cgatttgcag taattgcagt tgtgtctgct ggatgttgtt ttgcagttgg cttttttgag
     7261 tttgcagact ggaatcatcg atagtagcca gcaagtctcc ttttttcaca ggctgatttt
     7321 ctttgacgaa aacctttgtt acctgtcctt ctgtggctgc ttgtacgatt cgcaattctc
     7381 ctgtagggcg gatactacct tgacctttaa cagtgacttt atactcaatt atagaagcta
     7441 gtgcgatcgc tatccccaca ccagcgacaa tgaccaaact gcctaatctt gcccaattac
     7501 caataggcgg cagaaattca tcactttcaa ctatgggcag agagtctgca ttaatgtggt
     7561 taaccataac agttttttgg gtgttgctga atgagaatat ggatttgtgt cacgcattgg
     7621 cgctagcctc tccctttggg agaagggcgc aaaggcgcaa aggtacaaag aaaaattaag
     7681 gtaattttgc cgtttcattg gcaaattcag taactccgtt ttctgggtag agattagcaa
     7741 atgctggttg cagaacttcc attccgatga aagttagcgg gatttttcat caaaccattg
     7801 actcttgata tatccacacc gtcgagaaag tctaaatgct ccccaacttg ctgagataaa
     7861 atttctggag aaccttgaat tttcagttgt cctttttcta gcatcactat ccaatcagct
     7921 tgctgaatga ctttaggacg atggctaatt aaaatagtgg ttttaccttg gcgatgagtc
     7981 agcaattggt ttaagacttg ggcttcactt actgggtcaa gagcgccagt agattcatct
     8041 aaaatcagca cggctgggtt attcacaatc cctctcgcaa tggctaatct ttgcttttga
     8101 cccccggaga gattagcacc aaattcgcct aacaccgttt gatatttgtc cgggaggttg
     8161 ctgataaact catccgcccc agcaatttgg caagcttgca caatctgctc taaggtaata
     8221 tctggggaac ctaaacggaa gttgtccata atcgaccgac tccaaaagtg cgcctcttga
     8281 ggaactaaaa ccacttgttg gcggaagcag tctaaagcca aatcttgcag attataaatc
     8341 ccaaagcgaa tatttccaga ctggagggga tataaacctg caattaattt cgctaaggta
     8401 cttttaccac agccagattt accgatgagg gcaattactt taccgccagg aatagttagg
     8461 gagaaatctt gcaacagttc cactctgcct ggatgatgga agttgaggtt agtacaggtg
     8521 atgtctgcgt tactctgaat ttttacccaa ggctttcggc tattatctag agtttctggg
     8581 gtagcgtcaa taacttctgt gaggcgttgg gtagcagttt tggcacgggt gtattcatcc
     8641 acaaaatcga tagtagtttt aataaagctg ataaagttac cattcatgct gttaaatgcc
     8701 aagagcatcc caatagataa ttcctgctga attactagag tgctaccaaa ccagagcaag
     8761 gcaatactgc cgatgctgga tactaagttg gaaaatatgc cgttgagaat accaatttga
     8821 atagtccgaa agctcaggtt agcaaggcga ctaaatcgac tctggaactc ttcccagaat
     8881 tgcggagctg cggcggtggt tttgagagta atcgcacctt tgaaggtttc gactaaaacg
     8941 ccttggtttt ccgaggataa gactaaaaca ctacgaattt tttgctggag ggtgggcaat
     9001 gaaaggattg tggaaagagt cattaaaata gcgatcgcac cagctacagc tgtaagtttt
     9061 atactgtaga acaccatcaa gctcaaggaa actaaggcga taaataactg actcggtaga
     9121 ctgacaacag cttgagaaat taactgatta atttgttgaa tatcttctag gcgactgaca
     9181 atttcgccac tgcgacggga ttcataataa tttaaaggta agcggagaat ttgtcgtcca
     9241 aattcaaaaa ttagccctag ttgcagacgt tgtgcaaagt gagcgaccat atttgattgt
     9301 gctaactgta ggctactgct aactaggtgc ataacaacca cagcaatcac aacgctcgtc
     9361 aaaagttgtc tgtctccgcg aactagcaca tcatcggtga gaatttgcaa caaaaagggt
     9421 gaggtgagag acagcagacc tagaactaaa ttgagcaaca aggtttgagc aataacagca
     9481 cgataaggcc aaacacgccg caggaagcgt ccaacaccac caattttttc tggttgatct
     9541 tcctgagtaa caaagcggac tggatcaggt tctagtaaga gcatgacccc gtttgtccat
     9601 ccttccagaa gccacttttt ttctaaataa cgaatgcctg caccggggtc agcaatgacg
     9661 tatttattac cttgcttgcc atacaaaact acccaatgat aacctttcca gtggataatc
     9721 gctggcaggg aaatggtttt ttcgttgaaa acttccagag ttactttgac tgcccgtgca
     9781 ttgaaaccca gggcttctgc tccttgtttc aatcctaata gagtgcttcc ttcctgtcgt
     9841 gtgcctacag cttcccgaca tcggctcata ctaagaattt tgccataatg tttagcaata
     9901 gtcgcaagac tggcagcacc acagtcttct tcactatgct gttgaatcag ctggaatttc
     9961 atggtcagtt attgggtagc gttgcatact cgatttgttt ctatacttgg ctgttaacct
    10021 tgggaggttc cattactagc accgaaggaa caaactctgg tgctgctagg cgcaataatt
    10081 cataaccaat acctgctagc ccggtcatca gtcctggtgt ctcaactccc aagggaacgc
    10141 cacagagcca gccctgttga tcgatactgt cgagaatcac agatccaagg cggtcaactt
    10201 cagttttcca ctgaggctga tcaaggatga gactggcttg caacaggaat tctaagttgc
    10261 ccaaatctcc gtgacagagg gagtggttgc caccaaaacc atgagccaga gtggttttca
    10321 gcgccgtgtt aatttctgcc ctgatttgag aatcatcaag ataggaaaga caatgtaaac
    10381 gagccagccc aattcctggc gcaccattgc accaagctgt gataaggttg ggcgacactt
    10441 tctctgtccc ctgttctgga atttcagctt cacgccgatc aaaccaatta ccttcttctt
    10501 gacaaaagag ggtacgttca aattcaatgg cttgtctcgc agccgtttca aatcgctcct
    10561 cacctgtgaa ccgagctaat ttgaaaagag tccaagcgat acctgctgcg ccatgtgaaa
    10621 agcctgaaag cggactttta ctgaatccat tggcaaccca agctaaaccc ccctccatcg
    10681 gttgagcatg agccagaagc caatctccac attgaatagc cgtttgtaaa gttaacaaag
    10741 aaggggcatg gtgatgcaga ttaatcagac taacaatgca gcctgccgca ccaccaatca
    10801 catcaaactg ctgatcaaac tcgattaaac tggggaataa tgccgcagtt tcctccgctt
    10861 cagcgattaa ttccggttgt tcccaaagca ctcctaagtg agttagggtg tagattaatc
    10921 ccccccatcc ggtgaacgca ccaatagatg aaatggctga cttgacttgt tttacttcat
    10981 ggcgtagggt tttcattgct gcttgagcca agactgtgta acgctcttgg tgtgttaccg
    11041 ccccaagata agccaggaaa agggcaatgc ctggtagccc tgtataaagg tctaccccag
    11101 aggaagccag catccaatta cgttgatcaa ccagtgctaa tcccacccaa gtggcatctc
    11161 cgccaccttg tattgccaaa gactctagcc ggtctcccac tgctagggca gctgctagaa
    11221 actgactttg atcagcaaca gtatttgagg aatgagaaat tgttggtcga gagactgaag
    11281 cgtgtttaag ttcgtctccg gtgactgtag ttgccaagct tgcccggata taccaagttt
    11341 gttgagccag atcgttatca ctcaactgaa gaaaacgccg tgcaggagag gacattgttg
    11401 attcgtcaaa aaagttagca atccttttac cagagctact ccacaagtca ggtgaaccag
    11461 gataggtgaa aaatgccgga atatcacctt gctcaagatc ctctcgctcc gcaagaatga
    11521 tttgaaccaa atcaaaatca tttttgacct cactccaaag tttatcccac aggcgatcgc
    11581 ggtttaaggc gttgcgtaaa acgtctggat gaaagctctc atagagtaac attccataga
    11641 accgcgtagg acgcacaatg actcgcacct catcttcacc gaaagcagcc agaggtcctg
    11701 agtctgagag cagatgatcc cgatgtttca ggagtaatcc gtacatagcc gtgaaaccat
    11761 tgatcagcga cgagatgtaa tctagcagac gaaccgcctt tccttgcaac ttgggacggt
    11821 gatcaccttc tttcaaaggt aatcggctgc gtttgagttg catctggtct gtaccagccc
    11881 cttcccagta ggggagagga tcgggggata gctgaccggc ggtagcacca agaccactga
    11941 tatctaagcc ctctgattcg gaattgaacc aaagacgcat cggtagtagc ccgactttca
    12001 agacagactg aagaatttta ttgacaatca gttgctcggt attattttcg tctctcctct
    12061 ctctgggtgg atgaaagagt gcttccagat ccaggagtat aggatgttct cccaccgcaa
    12121 tcagattctc gaaatgaaaa tcagtcgcac tcaacccata caatattgct aaatatgccc
    12181 cttgtcgttc gtaaaatcgt tctatttcga tatttgtatc acaatcctct gctttgacga
    12241 actcaaccca accgtaggaa ccacgattaa ttactttgac agttcgcaag gttgggtggt
    12301 cacctcgttg attcaaccag gataaaagtt cctgaaaatg tatatcaatg gctaatgatt
    12361 tcggtttata aacgactttc cagccattag tgaatgtggc aatgagtact gaccttccac
    12421 cccgatgttt gtctcctaaa ttaccctcta gctgcaataa tttgtctgat gttgcgttgc
    12481 ttcccagagt agtttgaaga gtatgccaat cctgacaaag gtgggtaaga aactccagac
    12541 taaagctact cagatgatga atatgaatta ggagttggcg agctaaaaca gggtattctt
    12601 ctagaagggc aaaggccaca tccgaatggc gtagctgctg gataaagttc tcaaagcgct
    12661 cttggggggt atctccttgg agaagcccct gtagccgagc aacgttgagt tcaaggacta
    12721 aggtacgctt caagagctgg agcaattcct gagtccaatt gaccaaaact atggcttcaa
    12781 tggtgtctgg atcaaacggg agggaagagt gcgtctctcc tagcttctgg attccttgtc
    12841 ggaaatcatc aatggcttga cggagcaaag gctcgatgag ataaagaaat ccgacctctg
    12901 gttggttgtg ccattgctgg agtagcgcca cggcatcaaa agacatggga cgagcaaagg
    12961 cttgggtcag accttcgagc catgctggag gtgtagcgca acgatcttga acggcattta
    13021 tccgctcacc cagtaactct aggaactcat tttcattaat gccatcgagt gctagacgct
    13081 gatcaaaaaa cgcccctgtt gaaaattggg aacgccagcg ttctagaaga cgttcagcct
    13141 gtttctcatc tatgttcttt ttgggtgtct tagaggtatc agcacaaagc gctatacgtt
    13201 cttttaaagt cagggcgtga taccaagcct cggattgagt tgaggaggga gcggcaggta
    13261 gtttttggaa tggatcttct tttatggtcg agttgcttgt actttgtgat agtgtcatac
    13321 tgtaccttta tttatgcgta tttatacgat agttattatt aaaagtgggt gagcaatgac
    13381 agctcaccca tgatcattag taaaggcttt gtggaaaagg aaatgccttt ctagtgatct
    13441 cgtgattacc tgctgaaaca gcaacaggtt gaagttactg acatggcgtt gctaatattc
    13501 ttacaattgg taagccatga cggcaggtaa gcagactgaa caggttggga tgcatatctt
    13561 gcctcgccac cgactaggtt ttgtatttcc tgatctgcca tctcaaggat cccggcgggg
    13621 ttttcaggca gcattgagcg ctgttcttca cttaggctgt tgcgatattc ttcatctttc
    13681 caagcgcgga taatgtcgat attagacatg atttttttcc tttgatgttt tgtaatgatt
    13741 tttaggaata gacctttgcc ttcaggtatt aacctgtgct tttgtctctc tgtggctgta
    13801 tacgatggct ttatatgttt atgcaccagg gcgtgtcaaa aattaattga cggatagagg
    13861 cgcgtcaatt taatcccggc attttcagta gtaaattgcc aatctacttt acaattggca
    13921 aacaaactct gtgctggctt cagggagaat tacccaagat tccttcaagc aaggcttaat
    13981 ttcgtttttt ttaggacttg tggcacagtt tcatgggaaa tgctctctac atatcccaat
    14041 accactatcg gatctgctaa taaccgtaat gtccaatggc tatatccgaa tggtaggcta
    14101 ctgcaaaccg tcgctactaa atgggcttct gtttctccat ctagccgtcg taacttcaac
    14161 gaatctcgct gacgaggtac aagggcatta tctcagcttt cttccacaaa cctttgccgg
    14221 agacgctcat ggttctggtg ctgatattga aaatagcgct gattgactga tctttccatc
    14281 ccccatctgg gtgattcaca tctgctttca ggaggatctg agcatgattg attttggcga
    14341 cggcggcttt acctattttg ataattgttc taaagtttgg cgttcttcag gagtcaggga
    14401 gatcatgtat tttttgcaca tttggcaaaa tacctggagt cctgtgtatt agaattaagt
    14461 agatattcgt aaactctaaa ttttatatac tcaaaaaaac tttatgcaac cccgacaggg
    14521 cattacggaa atattctcga cttttgtgca gttggatgca gataggttca acgtctgggc
    14581 aacagatagc aaactccagc gaagtataaa aagatgcgta gaagcatacc cccaacagaa
    14641 atctgataat ttttgggtgc tttattggta caaaatctgg gaaatagaat ccaacccttt
    14701 agctgtggga catatttcgg cttatttaca agaagtctgc tattgggttt ctcgaaaaat
    14761 agccgtaaat tttatcagtc aattttctat tgctgattgt tttcagatgg cgatttcctg
    14821 tatttataaa attctcaaaa attttaatcc tgaatacagt acaaatttaa aaagctatgc
    14881 agaatatgct tttgagcgtt tcctcaagga cacattacgt ctaggaaagg aagcagatat
    14941 atgcacagat tgggcgttac tgcataaaat tagccgcaag cggttggtaa attctttaac
    15001 taatgcgggt tttaatagtc aaattagcaa taattacgtc ttagcttggg aatgttttca
    15061 ggaactttac accagtgaca gcaaaactat ccgtcaattg ggaaaaccag atagtaccag
    15121 atggcaagca atgactcagt tgtacaatag acaaagcttg agccaagtaa ctccagaaac
    15181 tttagaaaaa tggctgagta cctgcgctaa agctgtacga gactttcttt accccaagtt
    15241 tgtctctgtc gatgctttct ttaatgggca agaatcaggt aatctattag atacattacc
    15301 cgcagattta ccagcatcct tactcacaga aattattgcc caagaagagt ctacaaccag
    15361 agaaacacaa caagcccaat taaaccaagt tttgatggat gctttagcag cgttggatat
    15421 tcaggctcaa aaattactcc aagcttatta tcagcaacag ctgactcaac aacaaatctc
    15481 ggaacagcta gaaattaaac aatatactgt ctctcgccgt cttagcagca tcaagcgctc
    15541 attactcact accctaacgc agtggagtat cgatagtttg catatctcgc ctacacccgt
    15601 cgtagtggat gcaataagta aaagtctaga agaatggctc aaaaattatt atagccatac
    15661 ccacccacct ttaaagtaaa acctcttgag attatgtttg atgctgctgt tgctttcgct
    15721 gttgataatg acttggttct agaaatggtt ccgacatcag aacctctaga acataattat
    15781 tctacccctg gtggttatca acgtgcttgg ctcaatcaaa tttgtttaac tacattttta
    15841 gattggttac aaggggaaat tacacccaat gggagagtgc atcccaacag ggctacgtta
    15901 ccgagttttt gggaagtagt taatggtaca gctatcagtt ttgataactc tcgcttggtg
    15961 ttacttccta ctttagcaat ggatgtagat gagttgcgcg tacctcaaga atgggtggat
    16021 attcccgaat gggtggctga ttattactta gcggtacagg taaatcctga tgaagggtgg
    16081 atgagaattt tgggctatac aactcaccaa caagtgaaaa ctttgggggt ttatgatcca
    16141 ggcgatcgca cttacagttt agaatcagat catctcatcc cagacttgaa tgtgctttgg
    16201 gtaactcgcc aattgtatcc tcaagaaatc agacgtgcaa acgttgcacc cctaacacct
    16261 ttaccccaaa ctcaagcagc caatttactg gaaagattaa gtcacgcaga tatcaaattt
    16321 ccccgcttag aagttccctt tcccctttgg gctgcattaa tcgctcatgg cggttggcga
    16381 caaagcttgt atgaattgcg ccaaggaatt tcccaaccag catcaattgg gcggtggctt
    16441 caagacggcg tatcaaattt ttctcaacaa cttggttggg aaatacagca atttacagcc
    16501 ttaccttcag gaatgaggag tcgagaaaat caaacggcta ttttaggctt atctcgccaa
    16561 atactcatag ctgataatac ttatgaatta cgagttttcc ctaaaggtaa tccagaagaa
    16621 caaatttggc gttttgaatt acgcaatgca aacccagaaa atatgattcc tgtagggtta
    16681 caactcaaac tactcacaga agacttacaa ccatttccca acaatgaaga tacagcaaca
    16741 acgcctgtag aacaattgta tgtagaagta atgttagaac ccggtgaagg gttagtttgg
    16801 gaagtcacac ctttaccgac agaatatgag cgggaaatct tacgttttta atggacatat
    16861 tactaaatga ttcgaaaaaa ttattttttt ttgaaccaca gagacacaga gacacagaga
    16921 aatacaaaaa tcacaaactc ttcctctgga gcgactctgc gcctctgcgt gaactaaaat
    16981 atttgatgtt ttttacttta aaggtgtggg aagtggtaaa aaagaaagaa tatagaaaaa
    17041 gcaagttaat aaatttctgt ttgctggctg tgctaagtat atctatatgt attgtcgtga
    17101 ctccagcttt cgcaaatatt tctcaaaatc taataaatca agattcaact tcaaaaatcg
    17161 ccgaaagcca aatactatat gaaaggggta gatttgcaga tgctgtgcaa attttacagc
    17221 aagcggtgga agaatatcaa cagcagggag atactttaaa acaagctgtt gctttgagta
    17281 atctctcact ggcgtatcaa caattaggtc attggcaaaa agcgcaaata gctattacca
    17341 aaagcttgaa tttacaagaa ttttcccaaa aagaaagaac gaatcaaaat ttagccctct
    17401 tggctcaaag tctagatatt caaggacgct tacaactttt aacaggaaag tctgaagctt
    17461 ctcttgctac ttggaaaaat actgaagata tttacaccaa gctagaagat aacaacagta
    17521 ttgctttgac gctactttat caagctcaag cattacgcaa tcaaggtttt aataagcaag
    17581 ctatggagaa actatcacag ataaataaaa ctttagcgtc tcagggtgat tctttagcca
    17641 aagcagcagt ttttctttct ctaggtgcta cattagaaaa tgtgggagag ttagaaaaat
    17701 ctcgcctagc tttagaacaa agtttagaaa tttctcaaag gcttaaatca ccgcaaaata
    17761 tagctttaag cctgctgagt ttaggtaata atgctcggat tcaacagaaa aatttgcagg
    17821 cgatcgcatt atatgaaaaa gcatttgaaa tatcaacttc acccctgaca aaagttcaag
    17881 cccgactcaa tcatctgaat ttacttattg aagaaaaaga atttgcagcc gcacaaattt
    17941 taattcccga aattcaatct ttacttgcag aactaccagc ttcacgccca tcaatttacg
    18001 cccgcattaa ctttgctcac agcttgggta gtttaaatac tcccaaagct caaattgctc
    18061 aactcctggc tacatctgta caacaagccc ggagcttaga tgatatccgc gccgaagcct
    18121 acgctttagg caatttgggg agtttgtacg aaaaaacaca acaatttgct gaggctatga
    18181 agctgacaga acaggcgttg attttggctc aaactagcaa cgcgccggaa atcaagtatt
    18241 tgtggcaatg gcaaatgggt agactattaa aagcccaagg taatttcact ggtgcgattg
    18301 cgcggagcgc agtgccgaag gcaatcgccg aatatgatgc agcaataaaa acattagaat
    18361 ctctacgtgc tgacttagta gcagtcaatc aggatatcca gtttaatttc cgagacaatg
    18421 tagagccaat ttatcgtgag tcggtagctt taatactgca aaattctggc gaaaaaccaa
    18481 acgttaaaac attagataaa gctagaacac gaatagaagc tttacaatta gcagaactag
    18541 ataacttttt ccgagaagct tgcttacaag gagagagggt acttttagat caattagtag
    18601 acaaagaaaa tcccaacact gccatactct acccaattat tctgccagaa gaactgcaag
    18661 tcattgttaa aattcctcaa caaccactgc gccatcatac agttaaaaaa tcccaagtcg
    18721 aagttgagcg cactttggca aaactgcgag aatatatttt agaaccagat agaacagaag
    18781 aagtacaagc cctttcccaa caagtctata actggttaat taaacctatt gcatctgact
    18841 taaccacaag taaagttaat accctggtat tcgtactaga cggagcatta agaaatatcc
    18901 cactagcagc tctttatgac ggcgagaaat atttagtaga aaaatacgct gttgcgctga
    18961 gtttagggct tgggctatta gctcccagac ctctagctca aacaccagtg aaagtcctcg
    19021 ctgctggttt agtgcaacca ccgccgggtt tccccacatt tccaccctta ccaggaatca
    19081 agttagaatt tgacttaatt gctcaaacag gagcatctac taaacaacta ctagatcaag
    19141 actttaccag caatactcta gaaaacaatg taaatactgt accttttaat gtcttacatt
    19201 tggcaactca cgggcaattt agttctcgcc cggaagagac tttcatttta gccaatgatg
    19261 gtagcatcaa tgttttacag ttcgataact tactccgcag tcaaattgac aatagtagac
    19321 aaatcttaga attattagtt ttgagtgctt gccaaactgc cacaggtgac aatcgggcta
    19381 cattagggtt agcaggcgcg gctattaaag caggcgcacg cagcacaatt gcgtcactct
    19441 ggcacatcaa tgatcaatct acagctatct taattggtga attttacaat gaattagtca
    19501 atagtaaagt gactaaagcc gaagccttac gtcgcgctca agtaaaatta ttaaccgagt
    19561 atcctaatta tagtcgccct ggttactggg ctgcctatgt attagttggt aattggtttt
    19621 gagcaatcag gtgatattaa tgctgccaaa aatacgtatt tttctggttg tctcccagga
    19681 tatttttatt tagcagcatc acttctagac ctctgccaga tcaagccgaa gaagtagcaa
    19741 ctgtatgaaa tactaaaagc gcacttaccg taatcggatg atggcagtga tacatttaat
    19801 tattgttata aaaaaatact tacttgtgtt ttttaactta tacttctaca atacatgaaa
    19861 atccagcaaa taaataccta acaatgtccg aaaacttttg gaagtgggca gttatattag
    19921 caaccagtgg tgtgacaact accttgagtc atgctgcgat cgctcaaatt gtccccgatg
    19981 ctacattagg tgtagaaagt accatactca caccgaatgc cgatgtacgt ggtttacctg
    20041 cttctctcat agaaggtgga gccaccagag gcgtaaacct cttccaaagt ttctcagaat
    20101 tcaacgttgg cgatggacaa cgagtctact ttaataatcc cacaggtatt gaaaatattt
    20161 taactagggt aacaggtagc ggtctttcta atattttggg gacattgggt gtagacggac
    20221 aagctaatct atttttgctc aatcccaatg gaattgtttt tggtccgaat gcacagttag
    20281 acgtagccgg ttcgtttcta ggaactacag ctaatagttt tgtatgggaa aatggcttga
    20341 gcttcagcgc tacaaaccca gaacccgcac cattgattgc tgtcactttg gttccagggt
    20401 tgcagtatgg ggcaaattcg ccaggtgata ttacgagtac aggtaattta acagtagggc
    20461 aagatttgac attggcaggg ggtaatctta acttacaggg taatttacaa gcaggtagca
    20521 atttaaccct gaaagctcaa gatacagtaa atatcaatgg tagctactcg accactggtg
    20581 gtaattttaa cattgaacag ttagatggta gcttaggcaa tctagggagt aataatggct
    20641 ccgtgattag ggctagtggt gacgtgagct ttgcaagcta tacaggagct tcattgcaca
    20701 tcttagccgg gggaagtgta aatattccag gtcaggtaga aattactggt gcagctccca
    20761 caaatggact gactgagaca attacactat cagatgacac acaactaaat attgatggca
    20821 agaactctcc cacattagat atccgtgcag gtacaactgc ctttgatcct acaggactta
    20881 tacccaatcc tatcccaggt attgatcctc cagaacctat cgatcctatc ccaggtattg
    20941 atcctccaga acctatcgat cctatcccag gtattgatcc tccaccttca gaacctatcg
    21001 aacctatccc aggtattgtt actccaacaa actttggtaa cgagccaaca agcgcagata
    21061 ttaagatagg tggcattttt aatatttcag gagttgagtc taacagtggg cagatacttt
    21121 tgactaatca gtatcatccc aatgaattgg caggcaacat tgaatcgggt acaattttaa
    21181 cctatggaga tgtgataatt gatgcgagaa ataacattga aatcagtgga aatattgata
    21241 ctactattaa tgactctgta aacgccaacc ggacagctgg gaatattaaa ctttttgcag
    21301 aagaaaatat gatcattggt gctgacctgt tgtctaatgt caatggcacg gcagattcgc
    21361 cagtcggaaa tgggggtaag attacactta tcagcaagaa tggcacaatt gatacgacat
    21421 ctgggaatat aaactctaaa actcctaatg gaattgcggg agatgtaact cttgagtctg
    21481 ctggtaacat cagcgttggc aatatagaag cgtctagtaa tactaatagt aatgacccaa
    21541 attttagtac catcagcatg aaatctaggg agggttcagt tcagttagat gggactaggt
    21601 tgagtacaag caactctggt tctggcttgg ctggagatat cattatcgat gccaaagatg
    21661 aggtttctat tatcaatagt tcttctccag acgcaacacc cgaagaagac aacaaattag
    21721 gcatctttag tcaggggaac tcaggaaaca tatttattgg cacaaatttt actcctgaaa
    21781 aagtaacgat tgataattct gaactgaata gtgaagtgtt gggtggtaca ggcgatgcag
    21841 gcaatatcat aattaatgca ggctcaatat ccctgaacaa ttctcaactg atcacagata
    21901 acaacggctc tggactggag ggaagtatcg aagttattgc tagtcatcaa aatgtatcga
    21961 tggaaaatag cgcgataact agcgaaagta acaacgatca attccctgat gatgatgata
    22021 aagaaccctt tattctactt gcagctacac aaggatcagt tttgttgaac cagtctaggt
    22081 tgagtaccaa taattctggc tctggcttgg ctggagatat agaaatcaat gccaaaaatg
    22141 tggtttctat tctcaacagt aatgaaaata ataaaatatt caatgacaaa gaaacaggat
    22201 tatttagtga aggaaatttt ggacgtatct ttattggtct atctaatgta gctacagaag
    22261 atttatctcc gcaaaatgta acgattgata attctacttt gagtacaaaa aactctggtt
    22321 ctggctttgc tggagatata gaaatcaatg cccataattc aattgaaatc aacaatcatt
    22381 ctaaaatcaa aagcgaagga aattttggac gtatctttat tgggtcaagt gttctgtcgt
    22441 cagcagttgt acctcctaaa gaagtcatga ttaacaattc tgacttaaat accagtaata
    22501 gtgttacatc tggaggtgat agccaaataa ttaatgatcg ttcgggagat ataagtatta
    22561 actcccaaga aaaaatttct tttaataatg ctttaataac taccagcacc tttggagaag
    22621 gaaatgcggg aaagattgat atcaatacca atggttctgt gtctgttgac aatgccaaaa
    22681 ttttcagcag tgttcgtaat ggagcaatag gcaatgctgg caacatccag attcaggcag
    22741 attcacttga cttaaaaaat ggtgctgaac ttaatactga tacctctgga gaaggaaatg
    22801 cgggatttat tgatatcaaa accaatggtt ctgtgtctgt tgaaaatgcc aaaattgtca
    22861 gcgaagttaa aacagaagga accgggaatg ctggcaacat caatattcag acagattcac
    22921 ttgacttaaa aaatagttct gaaataagta ccagtacatc aggaaacggc aacgcacaag
    22981 atattttaat caccgcaaat aaatttgttt ctctatccaa ctttagcaac atcaacagtt
    23041 ctgtaagaga agacggcaaa ggtgaggctg gagaaattac tattaatgct caaacttttg
    23101 aagctttaaa tgttgggcaa gtgctaacca ccactactga tggtaatgct ggaaatatca
    23161 cactcaatgt tacagataaa ataacaatct ctggcagttc tccagagtcc aatattcctg
    23221 agtcaatgcg tgaaaagctg atatcttttg atgatgagaa taagagcaaa atatatccca
    23281 gtggtttgtt tgccaatact acagttgaat ctactgga
//
