LOCUS       NZ_CP060140            20933 bp    DNA     circular CON 28-MAR-2024
DEFINITION  Nodularia sphaerocarpa UHCC 0038 chromosome, complete genome.
ACCESSION   NZ_CP060140
VERSION     NZ_CP060140.1
KEYWORDS    RefSeq.
SOURCE      Nodularia sphaerocarpa UHCC 0038
  ORGANISM  Nodularia sphaerocarpa UHCC 0038
            Bacteria; Cyanobacteriota; Cyanophyceae; Nostocales;
            Aphanizomenonaceae; Nodularia.
REFERENCE   1  (bases 1 to 5451672)
  AUTHORS   Ahmed,M.N., Wahlsten,M., Jokela,J., Nees,M., Stenman,U.H.,
            Alvarenga,D.O., Sivonen,K., Poso,A., Permi,P., Koistinen,H. and
            Fewer,D.P.
  TITLE     Suomilide, a new member of the aeruginosin family
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5451672)
  AUTHORS   Ahmed,M.N., Wahlsten,M., Jokela,J., Nees,M., Stenman,U.H.,
            Alvarenga,D.O., Sivonen,K., Poso,A., Permi,P., Koistinen,H. and
            Fewer,D.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-AUG-2020) Department of Food and Environmental
            Sciences, University of Helsinki, Viikinkaari 9, Helsinki FIN-00014,
            Finland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 3; SPAdes v. 3.11.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 77.0x
            Sequencing Technology             :: PacBio RSII; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022376295.1-RS_2024_03_28
            Annotation Date                   :: 03/28/2024 07:15:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,496
            Genes (coding)                    :: 4,450
            CDSs (with protein)               :: 4,450
            Genes (RNA)                       :: 60
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 11 of 46
            Pseudo Genes (incomplete)         :: 34 of 46
            Pseudo Genes (internal stop)      :: 13 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 12:16:42
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1568781
            Orig. end                         :: 1589714
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP060140.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This genome has a base modification file available.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..20933
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="terpene"
                     /region_number="6"
                     /rules="(PT_phytoene_like or phytoene_synt or Lycopene_cycl
                     or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA
                     or TS_Pyr4)"
                     /tool="antismash"
     cand_cluster    1..20933
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /kind="single"
                     /product="terpene"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20933
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="False"
                     /core_location="[10000:10933](-)"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..10933)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
     misc_feature    complement(1147..1161)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 21.93"
                     /tool="antismash"
     misc_feature    complement(2039..2052)
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: weak, score: 20.08"
                     /tool="antismash"
     misc_feature    2110..2126
                     /note="TFBS match to NtrC, Nitrogen regulatory protein C,
                     confidence: medium, score: 21.83"
                     /tool="antismash"
     misc_feature    complement(2268..2283)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 21.91"
                     /tool="antismash"
     gene            2335..4023
                     /locus_tag="BDGGKGIB_RS06315"
                     /old_locus_tag="BDGGKGIB_01284"
     CDS             2335..4023
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012407708.1"
                     /locus_tag="BDGGKGIB_RS06315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BDGGKGIB_01284"
                     /product="NAD(P)H-quinone oxidoreductase subunit 4"
                     /protein_id="WP_239730680.1"
                     /transl_table=11
                     /translation="MMADQFPWLTAIVLLPFIAALVIPVLPDKDGKWIRWYALGVGVVD
                     LILMCYAFWHNYDASNATFQLEENYTWMPQLGLNWAVAVDGLSVPFVLLAGLVTTLAIF
                     SAWQVDRRPRLFYFLMLVLYSAQVGVFVAKDLLLFFIMWEVELIPVYLLICIWGGQKRR
                     YAATKFLIYTAAASIFILVAALAMGLYGGGNVSFDLVDLGLKDYPLNLELLLYAGLLIA
                     FGVKLAVFPMHTWLPDAHGEASSPVSMILAGVLLKMGGYGLIRLNLGILSDAHIYFAPV
                     LAILGVVNIIYGALNSFAQGNMKRRLAYSSISHMGFVLLGIASFTDLGISGAMLQMISH
                     GLIASVLFFLAGVTYDRTRTMIMDDLGGLGQVMPKVFALFTISAMASLALPGMSGFVGE
                     LAVFVGITTSDVYSSAFSIVTVFLAAVGVILTPIYLLNLLRQVFYGTSATLICDVNNAA
                     FENQEDEGTACFGTDCLLPDETVYSDAKPREVFIAACFLVLIIGIGFYPKLAMQMYDVK
                     TVAVNAQVRQSLMTISQTNPRVYAQGLLVPNIPSAEVAPVLGVVK"
     gene            4080..4490
                     /locus_tag="BDGGKGIB_RS06320"
                     /old_locus_tag="BDGGKGIB_01285"
     CDS             4080..4490
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006196188.1"
                     /locus_tag="BDGGKGIB_RS06320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BDGGKGIB_01285"
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="WP_239730681.1"
                     /transl_table=11
                     /translation="MKRRDFINWVGLGAIISSLPVAIAACSDQTIASDDWQTVGTVAEL
                     DKNGQLSVKNLPSVNVLVVGTSKSENLIAVNPTCTHSGCTVTWQTEGNKFNCPCHGAEY
                     GSDGQVQKGPATKALKTYTAKIENNSVLVKQN"
     gene            4516..5829
                     /locus_tag="BDGGKGIB_RS06325"
                     /old_locus_tag="BDGGKGIB_01286"
     CDS             4516..5829
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006196186.1"
                     /locus_tag="BDGGKGIB_RS06325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BDGGKGIB_01286"
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="WP_239730683.1"
                     /transl_table=11
                     /translation="MVNHINQLLVQAQAAADAADWSLLTQYLQQLILKLDSTQPEVDQN
                     RQDVLKLALSILDMGDFQQRWDIAKILRQLGNIAIPPLIEIVEDEDAEEDLRWYAVRTL
                     GEFQQPDAIISLVDLLKTSENQELKAMAASALGQMGTAAIKALTALLAEEETRVLAVRS
                     LAYIRTQETITPLLSVVEDSQAAVRSATLEALSSFHDQRVPPVLLKALDDVAATVRRAA
                     VQGLGFRPDLSTELDLVKRLQPRLSDFNLEVCCAAAIALSRMGTDEAAKHLFEVFISPN
                     TPLKLQLEIIRALVWVETLSGLEYLQESLYHTTSEILWQEIITVLGQVQKPQLMTSAAK
                     ILLAVLQSQHLVTVANPLGARIKSAIALGLGQLGNNQAVEALILLLADANQIVRLNAIA
                     ALKKLDGELAYQQLQQLAHNSTLTPDLQQGIAIALAEW"
     misc_feature    6006..6024
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: weak, score: 23.7"
                     /tool="antismash"
     gene            6202..6972
                     /locus_tag="BDGGKGIB_RS06330"
                     /old_locus_tag="BDGGKGIB_01287"
     CDS             6202..6972
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804066.1"
                     /locus_tag="BDGGKGIB_RS06330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BDGGKGIB_01287"
                     /product="hypothetical protein"
                     /protein_id="WP_239730685.1"
                     /transl_table=11
                     /translation="MNSIFPVYDTLREGKGLFYLSSVAVAALTILGSGLPAIAETVDTQ
                     MLAESSVNATSSDAMTLQQFPNAQTEEVANRTLTPVPGTAVTSSVALIPESKLTVQSAP
                     TQVAQADIDVRRSSPGVRNYVGFAGNIGIAGGDSSLGDGNFAIVSKVGLTNNLSIRPSG
                     VFGNDTTILVPITYDLKIQQADPFDEPLGIAPFVGVGAALKTGDDSKTALLITGGIDFP
                     LNPQFTATASVNAGFFRETDVGLLLGIGYNFSGF"
     gene            7334..7939
                     /locus_tag="BDGGKGIB_RS06335"
                     /old_locus_tag="BDGGKGIB_01288"
     CDS             7334..7939
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006196182.1"
                     /locus_tag="BDGGKGIB_RS06335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BDGGKGIB_01288"
                     /product="hypothetical protein"
                     /protein_id="WP_239730687.1"
                     /transl_table=11
                     /translation="MNQPKETSDNTTNFVLEKQKEKELTHDVLLLLQNLISREETTVKL
                     VLDSLYDVGSINLINHKFRSRTLNRTLKLISRTSKPVFRIFAWRWFQKNGSQLITNWLE
                     SKVTFKSTEPPQVASAQLVVENPEVKLNSLTKPQYQLQEVKHLRSQVTLLTGILAGVMT
                     VFGGSFVWLGYSLERSHMQRIVDLQTQVKTLEASVNEP"
     gene            complement(8166..9473)
                     /locus_tag="BDGGKGIB_RS06340"
                     /old_locus_tag="BDGGKGIB_01289"
     CDS             complement(8166..9473)
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1282:
                     extracellular ligand-binding receptor"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006196181.1"
                     /locus_tag="BDGGKGIB_RS06340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BDGGKGIB_01289"
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="WP_239730688.1"
                     /transl_table=11
                     /translation="MPRINTALALSIATIAAGLLLGACDNANNTNSTTEVTPGNTTDSS
                     QGLKIGSLLPATGDLASIGQQMAGSVPLLVETVNACEGVNGQPVTLVEVDDQTDPRAGV
                     AGMTKLATLDKVAGVVGSFASSVSTAAVSVAVPNQVMMISPGSTSPVFTQKAQKGDFKG
                     FWARTAPPDTYQALALAQLANQKGFKRVSTVVINNDYGVGFEKAFVETFEKLGGTVVNK
                     DKPIRYDPKAQTFDTEAAAAFAGKPDAVIAVLYAETGSLLLKTAYQRGLTEGVQIMLTD
                     GVKSPNFPDQVGKDADGKYLLAGAVGTVPGSDGKALADFNKLWQEKKGTSPGEYAPQAW
                     DAAALLVLAAQAAKENTGVGISNKIREVANEPGIEVTDVCAGLKLLREGQDINYQGASG
                     NVDVDANGDVVGVYDVWSVEADGKIKVIDKVNPSPK"
     tRNA            complement(9701..9792)
                     /anticodon=(pos:complement(1578537..1578539),aa:Ser,
                     seq:gct)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="BDGGKGIB_RS06345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="BDGGKGIB_01290"
                     /product="tRNA-Ser"
     gene            complement(9701..9792)
                     /locus_tag="BDGGKGIB_RS06345"
                     /old_locus_tag="BDGGKGIB_01290"
     gene            complement(10001..10933)
                     /gene="crtB"
                     /locus_tag="BDGGKGIB_RS06350"
                     /old_locus_tag="BDGGKGIB_01292"
     CDS             complement(10001..10933)
                     /EC_number="2.5.1.32"
                     /GO_function="GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /GO_function="GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="crtB"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: PT_phytoene_like"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: phytoene_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017320902.1"
                     /locus_tag="BDGGKGIB_RS06350"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BDGGKGIB_01292"
                     /product="15-cis-phytoene synthase CrtB"
                     /protein_id="WP_239730690.1"
                     /sec_met_domain="phytoene_synt (E-value: 1.1e-94, bitscore:
                     314.7, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="PT_phytoene_like (E-value: 1e-84,
                     bitscore: 282.4, seeds: 61, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLQLPDSPPRMKTLVSVDESYKLCRQLIAKYSATFYLSTLLLSEE
                     KRPAIWAIYAWCRRTDELVDGPGCAMTTPETLDLWEQQLESIFAGHPVENYDVALADTV
                     RRFPMDIQPFRDMIAGQQMDLYRSRYETFEDLYLYCYRVAGTVGLMSTSVMGLDETRNT
                     APWNCQQQPYMPTAEEIALGIAKQLTNILRDVGEDAQRGRIYIPLEDLARFNYTEQDLF
                     KGVVDDRWRSLMQFQINRARQFYTKAQRGITYLSTDARWPVWAALMHYSRILTIIERND
                     YNVFTQRAYVPQWQKLRSLPVAWMRSQVL"
     gene            complement(10917..12359)
                     /gene="pds"
                     /locus_tag="BDGGKGIB_RS06355"
                     /old_locus_tag="BDGGKGIB_01293"
     CDS             complement(10917..12359)
                     /EC_number="1.3.5.5"
                     /GO_function="GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA]"
                     /GO_process="GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="pds"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1222: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015083306.1"
                     /locus_tag="BDGGKGIB_RS06355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BDGGKGIB_01293"
                     /product="15-cis-phytoene desaturase"
                     /protein_id="WP_239730692.1"
                     /transl_table=11
                     /translation="MRVAIAGAGLAGLSCAKYLTDAGHTPIVLERRDVLGGKVAAWKDS
                     DGDWYETGLHIFFGAYPNMLQLFKELDIEDRLQWKEHSMIFNQPEAPGTYSRFDFPDLP
                     APLNGVVAILRNNDMLTWPEKIKFGLGLIPAMVQGQKYVEEMDKYSWTEWLRKQNIPER
                     VNDEVFIAMCKSLNFIGPDEISATILLTALNRFLQEKNGSKMAFLDGSPTERLCQPIVD
                     HITQGGGEVRLNAPLKEILLNPDGTVKGFLLRGLNGAEDEVLTADLYVSAMPVDPLKVM
                     LPEPWKQFECFQKLKGLEGVPVINVHLWFDRKLTEIDHLLFSRSPLLSVYADMSNACRE
                     YANSERSMLELVLAPAKDWITKSDEEIVTATIAELEKLFPDHFGQENPAKLLKSHVVKT
                     PRSVYKATPGCQQFRPSQKTPIANFYLTGDYTMQRYLASMEGAVLSGKLTAQAISQALP
                     VANSSDDLQTPTRPPATNAATA"
     misc_feature    12380..12389
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: strong, score: 18.31"
                     /tool="antismash"
     misc_feature    complement(12870..12886)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 25.97"
                     /tool="antismash"
     gene            12924..13751
                     /locus_tag="BDGGKGIB_RS06360"
                     /old_locus_tag="BDGGKGIB_01294"
     CDS             12924..13751
                     /EC_number="2.4.-.-"
                     /GO_function="GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006198229.1"
                     /locus_tag="BDGGKGIB_RS06360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BDGGKGIB_01294"
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="WP_239730693.1"
                     /sec_met_domain="Glycos_transf_2 (E-value: 8.4e-21,
                     bitscore: 72.6, seeds: 256, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQTPTTENCSNSDEIIVSMLEHITPLILTYNEAPNIRRTLEHLTW
                     AKKIIVIDSYSTDTTLEILNSYAQVQVLKREFDTHGKQWNYGLEQVKSPWVLSLDADYI
                     VTDDLISEMAALPVDGEIDGYFVRFKYCVFGKPVRNTRILPARQVLFQKSKAIYLDDGH
                     TQVLQLTGKSAVLSGYLYHDDRKSFSRWLWSQERYTVLEAKKILETPINELDWVDRIRR
                     QKILAPGMVFLYCLIIEGWIFDGWHGWYYVFHRVLAETILAIRIIEAEKLKRD"
     gene            complement(13778..14674)
                     /locus_tag="BDGGKGIB_RS06365"
                     /old_locus_tag="BDGGKGIB_01295"
     CDS             complement(13778..14674)
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089: methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006198228.1"
                     /locus_tag="BDGGKGIB_RS06365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BDGGKGIB_01295"
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="WP_239732029.1"
                     /transl_table=11
                     /translation="MLKEVSKNCSQYFAQPNKIDSWDEDIIICQILNPTPAIQANSYYF
                     GHPEWGLNYFQSCHRNEKFVELWQAVIGSWQDKVVVDIGCGPGNVYASLKEFCGEPQLL
                     LGVDVSLGALKMARQLGYTAVLADAQNLPFVSGFADLVILNASLHHCDDMAKVLQEAAK
                     LVRPGGLLITDHDPQQSAYQFKGLGSLLWKLRLPIYKYMKRGGHSTAEEQFWSLGTEVH
                     HKASDGVTSEMFYDILKPMGFAVKVYPHNHTVGAEALKGNYGRPFWKYRLAQRLSGIDP
                     NSVEAALSLMCVATRLD"
     misc_feature    14673..14690
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 18.54"
                     /tool="antismash"
     gene            14974..15819
                     /locus_tag="BDGGKGIB_RS06370"
                     /old_locus_tag="BDGGKGIB_01296"
     CDS             14974..15819
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006198227.1"
                     /locus_tag="BDGGKGIB_RS06370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BDGGKGIB_01296"
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="WP_239730695.1"
                     /transl_table=11
                     /translation="MLTKTSKNYYAEQILNKLEQCKSALKKEFIIGNRINTCYLDDLLN
                     PEDVLKIYHAFPDKKNLLQLKDLREYKYVGIQLDQYHPLLEEIIYAFQDTRIVNLIAEI
                     TGLEQLMPDEYLYAGGVSLMEYGCFLNPHLDNSHDKDISNYRVLNLLYYVTPNWEEDYG
                     GNLELWDQNLNQPCRTIHSKFNRLVIMVTNKTSWHSVSPINYHGRRCCVSNYYFSPKPA
                     DTSKYYHVTSFRGRPEQNLRDIVLRGDASLRNLVRKIFKHGIKKPHFYRNRVLNNTASK
                     "
     gene            16174..17259
                     /gene="nagZ"
                     /locus_tag="BDGGKGIB_RS06375"
                     /old_locus_tag="BDGGKGIB_01297"
     CDS             16174..17259
                     /EC_number="3.2.1.52"
                     /GO_function="GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="nagZ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015140866.1"
                     /locus_tag="BDGGKGIB_RS06375"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BDGGKGIB_01297"
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="WP_239730697.1"
                     /transl_table=11
                     /translation="MAGLQELERFGHHLILGISGTSLNDEDKRILSELKPVGVIYFAKN
                     FLDVTPYAVWLENFKNLNDQIREYTERDSMFMTLDHEGGRVVRTPLPITRFPYALLCRT
                     QAKEVAKATALELKSLGINVSWSPVADIFSHPQNPIIGPRAFGRNPETTAEGVCEYYRG
                     LEEGGILGCAKHFPGHGDTSKDSHIELPILNLSLEDLRIRELIPFKALISGEIPLIMTA
                     HILFPQIDPQFPATLSPAILKKILREELGFEGVVVSDDLDMKAVSDMFTQSGTIARAFN
                     AGCDLFIVSRNINSLSIERTYQMAGDFADSLSRGSLDESVVTAARDRIEKLLAVTPQYL
                     IHALDKNILQQHAQLAIACCY"
     gene            complement(17281..17679)
                     /locus_tag="BDGGKGIB_RS06380"
                     /old_locus_tag="BDGGKGIB_01298"
     CDS             complement(17281..17679)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016861728.1"
                     /locus_tag="BDGGKGIB_RS06380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BDGGKGIB_01298"
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="WP_239730699.1"
                     /transl_table=11
                     /translation="MNGSNRLAKKSLPASQQIENAHAHDIEEQTNHTHEHGKSVHPHVH
                     SEESLRKIVNRLSRIEGHVRGIKTMVQHNSPCPDVLLQIAAVRGALDKVARIVLDEHLT
                     ECIARAAQEGNMEVEIQQLKAALDRFLP"
     gene            17958..19175
                     /locus_tag="BDGGKGIB_RS06385"
                     /old_locus_tag="BDGGKGIB_01299"
     CDS             17958..19175
                     /EC_number="3.4.21.-"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012409104.1"
                     /locus_tag="BDGGKGIB_RS06385"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BDGGKGIB_01299"
                     /product="HhoA/HhoB/HtrA family serine endopeptidase"
                     /protein_id="WP_239730701.1"
                     /transl_table=11
                     /translation="MQFSQISSSIRQFSSYVLALIIGVTLTVSTWQVLPSQAEPAPYSE
                     TAADASDIIPQGQSPANAAIGSSSFVTAAVNRVGTAVVRIDTERTITRRMDPFMEDPFF
                     RRFFGESLPQQLPSEQLRGLGSGFIIDKGGLVLTNAHVVDQADKVTVRLKDGRTYEGKV
                     QGIDEVTDLAVVKINPDKDLPVAPLGSSDAVQVGDWAIAVGNPLGFDNTVTLGIVSTLK
                     RSSAQVGIADKRLDFIQTDAAINPGNSGGPLLNERGEVIGINTAIRPDAMGIGFAIPID
                     KAKAIALKLQRDGKVIHPYLGVQMVTLTPELAKQNNTDPNSPIQIPEINGVLVMRVVPD
                     SPAASAGMRRGDVIVQIDREPVTSADKLQNLVENSRLGQLLQVKVQRGNQIQLLSIRTA
                     ELQDPA"
     gene            19496..20677
                     /locus_tag="BDGGKGIB_RS06390"
                     /old_locus_tag="BDGGKGIB_01300"
     CDS             19496..20677
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1039: aldo/keto reductase family oxidoreductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010996912.1"
                     /locus_tag="BDGGKGIB_RS06390"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BDGGKGIB_01300"
                     /product="aldo/keto reductase"
                     /protein_id="WP_239730702.1"
                     /transl_table=11
                     /translation="MLYRRFGRTELQMPVFSCGGMRYQFKWQDVPHSEIPADNQANLEA
                     TIRRSLDVGINHIETARGYGTSEMQLGQILPKFPREKLIVQTKVSPVADAQKFRKTFEQ
                     SLRNLQLDYVDLLGIHGINHAESFENTMRPGGCLDVARQLQKEGKVKFIGFSTHGSKEV
                     IIQAINTNQFDYVNLHWYYINQWNWEAITAANNHDMGVFIISPSDKGGMLYKPPQKLVN
                     ICAPLSPMVFNDLFCLSHQNVHTLSVGASRPEDFDEHLKTLEYLDQAEEILPPILSKLE
                     QEAIASLGEDWVKTWETNLPTWEKTPGEVNIRVILWLLNLAIAYDLVEYAKMRYNLLGH
                     GSHWFPGNKADKLDELDLSLSLANSPHAEKIPQLLAQAHQMLAGAEVKRLSKT"
ORIGIN
        1 ctggatgagt aaatctcgta atactggctg taagtcttcg cagggtatgc ctttttgtac
       61 gcagttgcct aaatgcgcgt ctttgccaac tgtgccaccc atatatatgt ctacgccttc
      121 tacaggtttg ccatcttttc tagctttggt tcccatcaag ccgatatctg caacttgagg
      181 ctgtccgcag gagttagggc aaccagtcca gtgaattcgc acgggacgag ttagggttaa
      241 ctcagatgct aaggcttgag ccatttccaa ggcgcggctt ttggtttcaa ttagggcaaa
      301 gttacaaaac tgtgcgcctg tgcaagaaac aagcgatcgc ctcaacatat ccgggttaac
      361 tgcaaacctc tgtagtagtg gttctgttaa aaatgtttcc aaacgcgagt caggaatatt
      421 ggggatgatg atgttttgct caactgtaaa ccgaacttca ccatcggcgt aaacctctgc
      481 tagactcgca atttcaaaca tatcttgagc agatagccgc cccacaggaa tatttaagcc
      541 tacgtagttt aatcctggct gcttctgttt atatacccca atgtggtcgc gtttttccca
      601 gtcgatttcg tctttagcgg ctgcgggtaa cagtgtttta cccaaacgct tttctacttc
      661 agcacgaaat ttttctatcc cccattcatc aagtagccac atcaagcgag acttgaggcg
      721 gttagcgcgt aagccgttat cacgataaac ttctaaaact gctctacata cagctactac
      781 atcctctgga gacacccaag cattgagagg aatcgccgcc tcacagcgtt tggctgagaa
      841 aaagccaccg acaaggatgt taaagccgaa tgtttgtgtt gagttaaagg ctggaataaa
      901 ggctaaatcg ttaatttctg cgtgaactga attatcacga ccacctgcga tcgcaatatt
      961 aaatttccgt ggtaaattac taaactctgg gtttccttta cctttgttgg tgagcatatc
     1021 ttgaatttgc tgcaccactt ccctggtgtc aaataactca tccgcatcta acccagccac
     1081 aggatctccc gtgatattgc ggacgttatc cattccagat tggacggtgg ttaaaccaac
     1141 aaagtcaaat ttctgaaata tatctggtaa atcttcaatt ctgaggccac gcagttgaat
     1201 attctgtctg gtagtaatat cagcactgcc atcatctccg taacgctgta atactgccgc
     1261 taaaacacgc aactgagggc tagttagaac accgttaggt atccgcatcc gcatcataaa
     1321 cttacctgga gtgacagggc gaaaaaatac acctagccac ttgaggcgat ggtcgcggtc
     1381 gtttacgtcc attgcttccc aacctagaga agcaaatttt tctatctcct ccttgacagc
     1441 aagcccatct ttctcggctt tgagtttctc aaacttattg aggttagttt tggtagtagt
     1501 ttctgtatct gtcatgaact gactctcatt gcttatggct aatccagcga gaacattgga
     1561 cgctagttgc ccaatatcaa ttaatcccta gtcttttgat tactgagtct aggaaacact
     1621 tatatatatc tatttgtggc taatttctcg tactccagag cctcacctgg tgactcatct
     1681 gctacttgat gacaagcaag atgcaagtct atcgagttaa ctcaaattgt agttagttgg
     1741 taaacggttg taatctttcg catttagaaa atctgtcaag caggtttggt tactgtttat
     1801 gtaacgattt cgatacttat caggttaagg tgagtttcta gttaaaatcg tataaattgt
     1861 tacggttttt tgatataaat gtaaaaactg tattgattca atgcaatttc tgcatgacag
     1921 tcttttttat ttcaaaaata atctttgaca cacgccgttt tcattcccgt atgtaaatcg
     1981 ggaagaagaa cgtatcgctt tagctttcac ggagcgtctc cccttgggct accgtgtaca
     2041 aacaagtgat tgaatcaccc caaatccttt aaaagtaggg tgtgttatgc cgaagggcca
     2101 ccgcaccatc ccagattttc ggtgcgttag gactaaagtc cataacgcac cctaccaaaa
     2161 gaaaatgagt gcaggtaata ttttccaact tgtgtgtaca ccgtagctcc ccaggggaga
     2221 tgcaagaaag gaaagctggt gttacacctt ttttgggatc aatccaggtt aaaaaattat
     2281 taaaaagttg aataatatga catgatctct aaataagtat tatttcttag tgatatgatg
     2341 gcagatcaat ttccctggct gaccgcgatt gtcctgctac cattcattgc tgctctggtt
     2401 atccctgtcc tacccgataa agacggcaag tggatacgat ggtatgctct gggtgtgggc
     2461 gttgtagact taatcttgat gtgctacgcc ttttggcaca attacgatgc cagcaatgca
     2521 acttttcaac tagaagaaaa ttatacatgg atgcctcaat taggacttaa ttgggcagtt
     2581 gcggtggatg ggttatcagt tccctttgta cttttagccg gactggtgac gacactggcg
     2641 attttttcag cttggcaagt tgaccgcaga ccccggcttt tctatttcct gatgctagtg
     2701 ctgtactcgg ctcaggtagg ggtatttgtc gccaaagact tgctgctatt tttcattatg
     2761 tgggaagtag agctaattcc tgtctatcta cttatttgta tttggggtgg gcagaagcgt
     2821 cgttacgccg cgacaaaatt tttaatatat acggctgcgg cttctatatt tattctcgta
     2881 gcagcattgg caatggggct ttacggtggt ggtaatgtca gttttgattt agttgatttg
     2941 ggactgaagg attaccccct gaatttagaa ctgctgctat atgcaggatt gctgattgcc
     3001 tttggtgtca agttggctgt gttccccatg cacacttggt taccagatgc tcacggtgag
     3061 gcttcgtctc cagtatcgat gattttggca ggtgtgctgc tgaagatggg cggatacgga
     3121 ttaattcgcc ttaatttagg aatcctttct gatgcacata tttactttgc accggttcta
     3181 gcgattctgg gagttgtcaa tattatctat ggtgctttga actccttcgc ccaaggaaat
     3241 atgaagcgtc ggctggctta ttcgtcgatt tctcacatgg gatttgtact gctgggtatt
     3301 gcgtccttca ctgatttggg tattagtggc gcaatgttgc agatgatttc ccacggttta
     3361 attgcgtcgg tgctgttttt cttggcaggt gtgacttacg atcgcactcg tacaatgatc
     3421 atggatgatc taggcggtct tggtcaggta atgccgaaag tatttgcact atttacaatc
     3481 agcgcgatgg cttctctggc tcttcctggt atgagtggct ttgtgggcga acttgcggtt
     3541 tttgtgggga taactaccag cgatgtctat agttcagcct tttccattgt gacggttttc
     3601 ctcgccgcag tgggagttat tctcactcca atttatctac tcaacctgct gcgtcaggtc
     3661 ttttacggca cgagtgcaac actaatatgt gacgttaata atgcagcttt cgagaatcag
     3721 gaggatgagg gaacggcttg ttttggtact gactgtctcc tacctgatga aaccgtttat
     3781 agcgatgcta aaccgcgtga ggtgtttatt gctgcctgtt ttttagtgct gattatcggt
     3841 attgggtttt accccaagct ggctatgcaa atgtatgatg tcaagactgt ggcggtaaat
     3901 gctcaggtgc gccaatctct catgactatt tcccaaacaa atcctcgcgt ctatgctcaa
     3961 ggtttgttag ttcctaatat cccatctgcg gaagttgcgc ctgttttggg agttgttaag
     4021 taattttgtt gacctgttaa aataatgctc aataagtatt acagagaatt tccacaacta
     4081 tgaaacgtcg tgatttcatc aattgggtag gtttgggtgc aataattagt agcttacctg
     4141 tagcgatcgc ggcttgttct gaccaaacaa ttgcatctga cgattggcaa acggtgggta
     4201 ctgtggcaga gttagataaa aatggtcaat tgtcagtcaa aaacttgcca tctgtcaatg
     4261 ttttagtggt gggtacatcg aaaagcgaaa atctcattgc tgttaatcct acttgtactc
     4321 actcaggttg caccgtgaca tggcaaactg aaggcaataa atttaattgt ccttgtcatg
     4381 gtgcagaata tggcagtgat ggtcaggtgc aaaaaggccc ggctacaaaa gcactcaaaa
     4441 cttacactgc caaaattgag aataattctg ttttagtgaa acaaaattag tcctccccac
     4501 tccctatttg caagcatggt gaatcatatc aaccagcttt tagtccaagc acaagccgca
     4561 gccgatgcag ccgattggtc attactgact caatatctcc aacagttgat attgaaacta
     4621 gactctaccc aaccagaagt ggatcaaaat cggcaagatg tgctgaaatt agcactctca
     4681 attttagaca tgggagattt tcagcaacgt tgggatattg ctaaaatatt gcggcaattg
     4741 ggaaatattg ctatcccccc attgattgag attgtcgaag atgaagatgc tgaagaagat
     4801 ttacgttggt atgcagttcg cactttgggg gagtttcaac agccagatgc cataatctct
     4861 ttagtggatt tgctgaaaac cagtgaaaat caagaactta aagcgatggc tgcatcagct
     4921 ttagggcaaa tggggactgc ggcgattaag gctctgactg cacttttggc tgaggaagaa
     4981 acaagagttt tggcggtgcg atcgcttgct tacattcgca ctcaggagac gattacacct
     5041 ttattgagtg ttgtcgaaga ttcacaagcc gcagtccgca gcgccactct cgaagccctc
     5101 agcagctttc atgatcaacg tgtaccacca gttctattaa aagctttaga tgacgttgct
     5161 gccactgtca gacgtgcagc agtgcagggt ttaggttttc gccctgattt atctacagaa
     5221 ttagatttag tgaagagatt gcaacccaga ttatctgatt ttaatcttga ggtttgttgt
     5281 gcagctgcga tcgctctttc tcgaatgggt actgatgagg cggcgaagca tttatttgag
     5341 gtgttcattt ctcccaatac accactgaag ctgcaattag aaattatccg cgctttagtt
     5401 tgggtggaaa ctttatctgg tttggaatat ttgcaagaat cactctatca taccacatca
     5461 gagatacttt ggcaagaaat tattacagtt ttggggcaag tccagaagcc gcagttaatg
     5521 acatcagccg ccaaaatttt gttggctgtt ttgcagtcac agcatttagt cacagtggca
     5581 aatcctttgg gagctagaat taaaagtgcg atcgccttag gtttggggca gttaggaaac
     5641 aatcaggctg ttgaagcttt gattttattg ttggcagatg ctaatcaaat tgtcagatta
     5701 aatgcgatcg ccgcactgaa aaaactggat ggggaacttg catatcaaca attacagcaa
     5761 ttagcccata attccacact cacaccagat ttgcagcaag gaatagcgat cgccctggca
     5821 gaatggtagc gcataactag gtccgcagga aaaaaatctc ccccggctaa aaaatagcac
     5881 agaggagatt tacagttaaa gttcgtcagg tgatcggaat agctacttat ttagtattaa
     5941 tcaccattac tttaattccc cctagagata gataaatatc atttgctctt caattcacca
     6001 ccaaataccg tatatttatc gagtgcgatc gcttattaac ttctcatttc taatacccag
     6061 tacccagtat ttttaacaag acttacgtct acttaaagat gtgataactg attttgtgat
     6121 gttacagttt gaatatcgcc caattttgtg gaatcatcag cacagaatgc cgattgcggc
     6181 catatttagt aaggagtgaa cgtgaatagt atttttccag tctacgacac gctacgcgaa
     6241 ggcaaaggtt tgttctactt gtccagtgta gctgtagcag ccctgacaat cttgggtagt
     6301 ggtctgcctg ctattgctga aacagtcgat actcaaatgc tagctgagtc ttctgtaaat
     6361 gcaacatctt ccgatgccat gactttacag caatttccca acgctcaaac cgaggaagtg
     6421 gctaatcgaa cactcacacc agtcccaggg acagcagtaa cttcatcagt cgccctaatt
     6481 cccgaatcaa aactgaccgt tcaatctgct cctacacagg tagcacaagc tgatattgat
     6541 gttcgtagat cgtctcctgg cgtgagaaac tatgttggtt ttgctggtaa cattggtatc
     6601 gctggtggtg actcatcttt aggtgacggc aattttgcca tcgtcagtaa agttggactg
     6661 acaaataacc tatccatcag accatcaggt gtgtttggta acgacaccac aatcctggtt
     6721 cccattactt acgacttaaa gatacagcag gcagatccat ttgacgaacc attagggata
     6781 gccccttttg tgggagtagg tgcagcgctt aaaacaggcg atgattccaa aactgctttg
     6841 ttaataactg gtggtataga ttttcctcta aatcctcaat tcacggccac agcttccgtg
     6901 aatgccggat tttttagaga gactgatgtc ggtttgttgt taggaattgg ttacaatttt
     6961 tctggctttt aagcacgtaa tatgaaaaat aaaaggcaaa agtaatttat acttttgcct
     7021 ttggcaattt taaagtttat taatttttaa tcaacactga atagattata caaataagat
     7081 aatagttata tcgagttata tcggagacat catgagtatc acttgaaaat acagagtttt
     7141 ttatcttgat ataatcccta aattctgcga taatatgcaa aatatctgct atcacagcta
     7201 aattaccaac agcaatacaa acagttgcca aataatgcga ctttcttggt tgggatatgg
     7261 ttttgatttt ggcaagaatt attccagttt atgatcacaa ataaatattc atgaaaaact
     7321 aacctttata tatatgaatc aaccaaaaga aacatctgac aacacgacaa attttgtgct
     7381 agaaaagcaa aaagaaaaag aactcaccca tgatgttcta ctattgctac aaaatttaat
     7441 ttctagagaa gaaactacag tcaagctagt attagattct ctgtatgatg taggatcaat
     7501 taacctgatt aaccacaagt ttcgttctcg gacgctcaat agaactctga aactaatttc
     7561 ccgaacatcc aagccagtat ttagaatttt tgcttggcgc tggtttcaaa agaatggttc
     7621 tcaattaatt actaactggc tagaaagcaa ggtgactttc aaaagcacag agccaccgca
     7681 agtagcctca gctcaattgg ttgtggaaaa tccagaggta aagctgaatt ctctgaccaa
     7741 accgcaatat cagcttcagg aagtgaaaca tcttcgctcc caggtgacat tgctcacagg
     7801 tattttggct ggagttatga ctgtgttcgg tggtagcttt gtctggttag gttattccct
     7861 agagcgatcg catatgcaaa gaattgtaga cttacaaact caggtaaaaa ctctcgaagc
     7921 cagcgtaaat gagccatagt agcgaaaagt cattagtcat tagtcattag tcattagtca
     7981 ttagtcatta gtcattagtc attagtcatt agtcagtgag ttttttcgcc caggatagct
     8041 tgcgtggcgt agctacgggg cggggtatac accctcaaca actaatgact agtgacaact
     8101 aaagtcctct ctacaagacg ctaggtgaac actaaaaacc tttaacttcc agcctgtggg
     8161 agctactatt taggactagg gttaactttg tcaatcacct taatcttgcc atctgcttcc
     8221 acactccaaa catcgtagac accgacaaca tcaccattgg catcaacatc aacattgccg
     8281 cttgcgcctt gatagttaat gtcttgaccc tctcgcagta acttcagccc cgcgcagaca
     8341 tcagtaactt ctataccagg ttcattagca acttcacgga ttttgttaga aataccaaca
     8401 cctgtgtttt ccttcgcggc ttgtgctgcg agtaccaaca aagccgcagc atcccaagct
     8461 tggggagcgt attccccagg tgaagtgcct tttttctctt gccatagctt gttaaaatct
     8521 gctaatgctt taccatcgga accgggtact gtacccacag ccccagctaa gagatatttg
     8581 ccatcagcgt ctttacctac ttggtcagga aaattgggtg atttgactcc atctgtgagc
     8641 ataatttgca caccctcagt caaacctcgc tggtaagcag ttttgaggag cagactacct
     8701 gtttcagcat agagaaccgc tatcactgca tctggttttc ccgcaaaagc agcagcagct
     8761 tcggtgtcaa atgtttgggc tttaggatcg tagcggatgg gcttatcttt attaactaca
     8821 gttccaccca atttttcaaa agtttctaca aaggcttttt caaaccctac cccatagtcg
     8881 ttattaatta caactgtgga aactcgcttg aagccttttt gattagctag ttgagctaat
     8941 gctaatgctt ggtaggtatc aggaggagct gttcgcgccc aaaagccttt aaagtcgcct
     9001 ttttgtgctt tttgggtaaa cactggactg gtactaccag gagaaatcat catcacttga
     9061 ttaggtacgg caactgagac agcagcagta gaaacgctac tagcaaagga accaactaca
     9121 ccagctactt tatccagagt tgctaatttg gtcataccag ccacaccagc tctaggatcg
     9181 gtttggtcgt ctacttctac gagggtgact ggttgcccat tcactccttc gcaagcgttc
     9241 acagtctcca ctagcaaggg aactgagcct gccatttgct gtccaatgga agctaaatcg
     9301 ccagttgctg gtaataaact accaattttt aacccttgac tactatcggt tgtgttccct
     9361 ggagtcacct cggttgtact attggtgtta ttagcattgt cgcaagctcc caaaagcaaa
     9421 cctgctgcta tggtagctat acttaacgct aaggcagtat taattcttgg cataattgac
     9481 ttttcactcc tcagatattt aggagcctaa aagtaagatt caaactaggt ttttaagtta
     9541 gcccaatcat cataggctcc agagaataaa taatagcatc tacccagagg agtaaaactt
     9601 ttaccaatac atcaatattt attggtttgt ttgatattag tttagctagg tctacccaaa
     9661 cttgagttct gggtaaacac taggttcaaa tcactaaaaa cggagaggga gggattcgaa
     9721 ccctcggtac ggagttacct gccgtacaac agattagcaa tctgtcgctt tcgaccactc
     9781 agccacctct ccaggtggtc tacagataac aacattagca gactttttcg agttagtcaa
     9841 gtctcagtga gcaaataatt cgtaattcgt aattaataga cagagctact taatttatca
     9901 atggataaaa atgaaatgcc gtcaatgttt caggttttcc tgaatttatt tatggggcgc
     9961 aaaacttacg aattacgaat tattttgact actgacctaa ttacaggact tgcgatcgca
    10021 tccaagctac tggcaaagaa cgtaattttt gccattgggg tacgtaagca cgttgagtaa
    10081 aaacgttgta atcgttgcgt tcaatgattg ttaaaattcg actgtaatgc atgagagccg
    10141 cccatacagg ccaacgggca tcagtggata ggtaagtaat acctctttgg gctttagtat
    10201 aaaactgtct agctctgtta atttgaaact gcatcaggga gcgccagcga tcatcgacta
    10261 cacctttaaa caagtcttgt tcggtgtaat tgaatcgcgc caaatcttcc agaggaatat
    10321 aaatgcgccc acgttgtgca tcttcgccca catctcgaag gatgttggtg agttgcttgg
    10381 caattcccaa ggcaatttct tcagcagtcg gcatataagg ctgttgttga caattccacg
    10441 gggctgtatt tctggtttca tccaaaccca tgactgaagt tgacattaag cccacagtac
    10501 cagcgacgcg gtaacagtag aggtataaat cctcaaaagt ttcatagcga ctgcgatata
    10561 aatccatttg ctgaccagca atcatatccc ggaagggctg aatgtccatg ggaaagcgtc
    10621 gaacagtatc agccaaagct acatcataat tttctactgg atgtccagca aaaattgatt
    10681 caagctgctg ttcccataaa tctagggttt ctggcgtagt catggcacat ccaggaccat
    10741 cgaccagttc atctgtacgg cgacaccaag cgtaaatcgc ccaaatagca ggacgttttt
    10801 cttcgctcag gagcaaagtg cttaggtaaa aagtcgcgga atacttggct ataagttgcc
    10861 gacaaagttt gtaggactcg tctacagaga ccagcgtttt catgcgcgga ggggaatcag
    10921 gcagttgcag cattcgttgc gggcggtcgg gttggcgttt gcaggtcgtc tgaggaattt
    10981 gctaccggga gtgcttgtga aatcgcttgc gctgtcagct taccagaaag tacagcacct
    11041 tccatactgg ctaagtagcg ttgcatggta taatccccag tcaaataaaa gttggctatg
    11101 ggggtttttt gcgaggggcg gaactgttgg caacctgggg tcgctttgta aactgaacgc
    11161 ggtgttttca ccacatgaga tttcagcaat ttagcgggat tttcctgacc aaaatggtca
    11221 gggaagagtt tttccaactc agcaatcgtg gcggtaacaa tttcctcatc cgatttggtg
    11281 atccaatctt ttgccggagc taagactaat tccagcattg agcgctcaga attagcatat
    11341 tcacgacaag cattgctcat atcagcataa acgctgagga ggggcgatcg cgaaaatagt
    11401 aggtgatcaa tttctgtcag tttacgatca aaccacaaat gtacgttaat tacgggtacg
    11461 ccttcgagtc cttttagctt ctggaaacac tcaaattgct tccaaggttc tggtaacatc
    11521 accttcaggg ggtcaactgg catagccgat acatacaaat cagccgttaa cacttcatct
    11581 tctgccccat ttaaccctct gagcaaaaat cctttgacag taccatcagg gttcagcaaa
    11641 atctctttta acggagcatt taggcggact tcaccaccac cctgggtgat gtggtctact
    11701 atgggctgac acaatcgctc tgtgggtgaa ccatctaaaa acgccatttt tgagccgttt
    11761 ttttcttgga gaaagcgatt cagggcagtt agcaagatag tggcagaaat ttcatccggg
    11821 ccaataaagt tgagtgattt gcacatcgca ataaacactt catcattgac tcgttcggga
    11881 atgttctgct tgcgtagcca ctctgtccaa gagtatttgt ccatttcctc aacgtatttc
    11941 tgcccctgaa ccattgctgg aatcagacct aagccaaatt taattttttc tggccatgtc
    12001 agcatatcgt tgtttctcag aattgccacg acaccattta agggggctgg caaatctggg
    12061 aaatcgaagc ggctgtaagt tcctggcgct tcgggctggt tgaaaatcat ggagtgttct
    12121 ttccactgca atcgatcttc aatatccagt tctttaaata gctgcaacat attgggatat
    12181 gcgccaaaga agatgtgtag acctgtttcg taccagtctc catccgagtc tttccacgcc
    12241 gccaccttgc cacccaatac atcccggcgc tccaagacaa tgggagtgtg acctgcgtct
    12301 gtgagatatt tcgcgcagga tagccctgct agtccagcac ccgcgatcgc tactcgcatt
    12361 tcaacttact gcctttcaat attttttgat ccttttgtcg ttctcattat acgttgcaat
    12421 ccgttacatt tggcaggggt agtgtgatcg atcaagcaaa aaccgctctc aaaagccatt
    12481 ttaatgagat acacccacag cagcagagcg ccagcatcat taccactcaa gcactcaatg
    12541 attcgtaagt tatctactaa cttttgccag tagtctcaaa tattctccag atatacgtta
    12601 atttatgtat cttttttggt aaagtccaac atattttttt atctagtgct ataattaaat
    12661 gtttttctag caatcactgt agtccacata cagtccttaa ataaaatatt tgttctcaat
    12721 ccagacgagt acgagaatca gcactcacaa gatgccccag aatagttagt gggtagtaac
    12781 aagcgacgac ggtaatcacc aaaattacga tctaactgcg gaaaaccatc taatttcctg
    12841 cctaaaaatt gacaaattcc aacttttgta tgttatccaa taacaagaat caatgacaaa
    12901 ctaaataaaa tagctcatct cctatgcaaa cacctactac agagaattgc tctaattctg
    12961 atgaaattat tgtatccatg ttagaacaca ttactcctct gattctcact tataatgaag
    13021 cgcccaatat tagacgcacc cttgagcatc tcacttgggc taaaaaaatc atagttattg
    13081 atagttatag tactgataca accctggaaa ttctcaactc ttatgcacaa gtccaggtat
    13141 taaaacgtga atttgatacc cacggtaaac aatggaacta tggcttagag caagtgaaat
    13201 ctccctgggt actttcccta gatgcagatt atatcgttac agatgattta atctctgaaa
    13261 tggccgcttt acctgtggat ggagaaattg atggctactt tgtcaggttt aagtactgtg
    13321 tctttggcaa acccgttcgt aataccagaa tactccctgc gcgtcaagtt ctgtttcaaa
    13381 aaagcaaagc tatttatctt gatgatggac atacacaagt tttacaactg actggtaaat
    13441 cagctgtact ctctggttat ctctaccatg atgatcgcaa atcatttagt cgttggttat
    13501 ggtcacaaga acgttataca gtactggaag ccaagaaaat actagaaact cccatcaacg
    13561 aacttgattg ggttgatcgt attcgcagac aaaaaatctt ggctcctggg atggtatttc
    13621 tctattgctt aattatcgaa ggctggattt tcgatggttg gcatggttgg tattacgtat
    13681 ttcatcgtgt tttagccgaa actatcttgg caatacggat aattgaagca gagaaattaa
    13741 aacgtgatta acgaaactag catacccata tttagaatca atccaggcgt gtggcaacac
    13801 acatcaagga caatgccgct tctacagagt tgggatctat acctgataat ctctgagcta
    13861 gacgatactt ccaaaatggc ctaccgtaat taccctttaa tgcttctgca cctacagtat
    13921 ggttatgggg gtaaacttta actgcaaagc ccattggttt gaggatgtca tagaacatct
    13981 ctgatgtcac accatcagag gctttgtgat gtacctctgt tcccaaagac cagaattgct
    14041 cctcggcggt ggaatgtccc ccccgtttca tatacttata aattggcaac cgtaatttcc
    14101 acaatagtga acctaaacct ttgaactgat aagcactttg ttggggatcg tgatctgtga
    14161 ttaaaagccc accaggacgt accaatttag ctgcttcttg caaaactttt gccatatcat
    14221 cacaatgatg caagctggca ttaaggatca caagatcagc aaacccagaa acaaaaggca
    14281 gattttgagc atctgctagc actgctgtat atccgagttg acgagccatt ttcagtgcgc
    14341 ccagagaaac atctacaccc agtaacaact gcggttcacc acaaaactct ttgagagagg
    14401 catagacatt accagggcca caaccgatat ccaccactac tttgtcttgc caactaccaa
    14461 tcacagcttg ccacagttcc acaaactttt catttctgtg acaggattga aaataattta
    14521 atccccattc tggatgccca aagtagtagc tgttggcttg gatagctggg gtgggattta
    14581 aaatctggca aataataata tcttcatccc agctatcgat tttgtttggt tgagcaaaat
    14641 actgcgagca gtttttactt acttctttca atatgtaatc cattcgttaa gcgtgtattt
    14701 acttaaaatg ttggtttttt gtgtcctggt actaagtata agaaattact tttattttga
    14761 aagataattt cttgaagaat tatgtatact catcgttgac tatcttaact tagtataaag
    14821 ttatcaaaaa aaatacagaa ataattaata tttttgtgaa ttaaaactgt ctcatataaa
    14881 aatttgattt tttttcctat ttagttatgc cgtttttgta aaaatgctat attatatgca
    14941 aatctttttt actaaatccg gaaaaaatta tcaatgctaa ccaaaacttc aaaaaattat
    15001 tatgccgagc aaattttaaa taaactagaa cagtgtaaat cggctttaaa aaaagaattc
    15061 atcattggca accgaataaa cacgtgttat ttagatgact tattaaatcc cgaagatgta
    15121 ttaaaaatat atcatgcatt tcccgataaa aaaaacttgt tgcagttaaa agacctgaga
    15181 gaatataagt acgttggtat acaattagac cagtaccatc ctcttttaga agaaattatc
    15241 tatgcctttc aggataccag aatagttaac ctgattgctg aaataacggg attggaacaa
    15301 ttgatgccag atgaatacct ttatgctggt ggcgttagcc tgatggaata tggctgtttt
    15361 ttaaatcctc atctggataa ttcccatgat aaagatatta gtaactatcg tgttttgaat
    15421 ttactgtatt atgtaactcc caattgggaa gaagattatg gcggtaattt agaactttgg
    15481 gatcaaaatt taaaccagcc ttgtagaact attcacagta agttcaatcg gctagtgatt
    15541 atggtgacta ataaaacttc ttggcactct gttagcccaa ttaattatca tggtcggcgc
    15601 tgttgtgtat ctaactacta tttttcccca aaacctgctg atacaagcaa gtattatcat
    15661 gttacttctt ttcgaggtcg cccagaacag aatctgagag atatagtttt gagaggagac
    15721 gcaagcttac gaaatctagt gcgtaaaatc tttaaacatg gaattaaaaa gcctcatttc
    15781 tatagaaata gagtattaaa taatacagct tccaaatgag agtagaagat caattcagtg
    15841 gaaataacca tgtcttgttg ccaaaattta ctttgttgac acttccacgc gataacgact
    15901 cggactcatg ggataacaga acagaagttt gccctcagtg tattgcccat ctagtaggca
    15961 actgaatttt gacaatttta tacattaagc atattatttt atttttttgc atatttgctc
    16021 cagatacata gctaggagca tgataaagtc aggcgattta tgaagttgcc aaaagttcaa
    16081 aaattagcat agaagtgtag gataatacct tatttctatt tgctcaaagt agaaagtccc
    16141 agttgttggt tacacaacta taggtaaagc attatggcag gattgcaaga gttagagcgc
    16201 tttggacatc acttgatact tgggatttca ggtacttcat taaatgatga ggataaacgc
    16261 atactgagtg aattaaaacc agttggggtg atttattttg ctaaaaactt tctggatgtt
    16321 acaccttatg cagtttggct agaaaatttc aagaatctca acgaccaaat cagagaatat
    16381 actgaacgtg actcaatgtt tatgacgtta gatcacgaag ggggacgggt agttagaaca
    16441 ccactaccaa ttacccgatt tccttatgct ttgttgtgta gaacacaggc aaaagaagta
    16501 gcaaaagcca cagcgttaga gttgaaatca ctgggaataa atgtatcctg gtcacctgta
    16561 gcagatattt tttcccatcc tcaaaatccg attatcgggc ctcgtgcctt tggtcgcaac
    16621 ccggaaacaa ctgctgaagg tgtctgtgaa tattacagag gacttgagga agggggaatt
    16681 ttaggatgtg ctaaacactt tcctggacat ggagacacta gcaaagattc tcatattgaa
    16741 ttaccaatac tcaatcttag tttagaagac ttacggattc gggaacttat accatttaaa
    16801 gcgctgattt cgggggaaat tccgttgatt atgacagcgc atatcctgtt tcctcagata
    16861 gatccgcaat ttcctgcgac actttcccca gctatcctga agaagatcct ccgggaagaa
    16921 ttggggtttg aaggtgtggt tgtatctgac gatctcgata tgaaagctgt ttcagatatg
    16981 tttacccaaa gtgggacaat agcgcgagcc tttaatgctg gctgtgattt gtttattgtc
    17041 tcgcggaata ttaattcatt atctattgaa cgtacttatc aaatggcagg agattttgct
    17101 gattcgttaa gtaggggtag cctggatgaa tcagtggtga cagcagctag agacagaatt
    17161 gagaaattat tggcggtaac tccccaatat ttgatccatg ctctggataa aaatatacta
    17221 cagcaacacg cacaattggc gatcgcttgt tgttactagg ggagtgggga gtggagaatc
    17281 ttaaggcaaa aaccgatcta aagcagcttt taactgttga atttccactt ccatattgcc
    17341 ttcttgagct gctcttgcaa tacactcagt taaatgttca tctaaaacaa ttctcgcgac
    17401 tttatccaaa gcgccccgca cagccgcaat ttgtaataaa acatcaggac aagggctatt
    17461 gtgctgcacc attgttttaa taccgcgaac atggccttct atccgagata gccgattcac
    17521 aatttttcgt agcgactctt cactatgaac gtgaggatgt accgactttc catgctcatg
    17581 agtatgattt gtctgctctt ctatatcatg agcgtgggcg ttttctattt gctgggatgc
    17641 cggcaaagat tttttagcta atcggtttga tccgttcatg ggttcatgtt gccaaagcac
    17701 tgttactatt aagatcctag cctagaagat aattggtaat cagaaaaata tttgtagcaa
    17761 ttaacgatcg ctttggttaa atgattccag ggtgaaaccc gaaccttgaa agggcgaatc
    17821 tccttaatag ctattttgtt aaatattata ttaattttta ttataggtag tttacgacca
    17881 atcatctgca attctgtaaa aataactgta aatggatttt ttaggcaaaa attaggctgc
    17941 catattaggt tgtaattatg caattctccc aaatatcaag ttctatacgt caattcagtt
    18001 cttatgtgtt agccctaatt atcggggtga cgctaacggt cagcacttgg caggtattgc
    18061 cttctcaagc agaaccggca ccttattctg agactgctgc ggatgcttca gatattattc
    18121 cccaagggca atcaccagca aatgcggcta taggtagcag tagctttgtc acggcggctg
    18181 taaatcgtgt gggaacagca gtagtaagga ttgatactga acgcactatt actcgtcgga
    18241 tggacccttt tatggaagac ccattttttc ggcgattttt tggtgagagt ttgccgcaac
    18301 agttgccttc tgaacaattg cgcggtctag gttcggggtt tattattgat aaaggtggtc
    18361 tggttttgac caatgcccat gtagttgatc aggctgataa agtgacagtg cgcctcaaag
    18421 atggtcgtac ctatgaaggc aaagttcaag gtattgatga agtcactgat ttagccgtgg
    18481 tgaagattaa ccctgataaa gatttacctg ttgcgccttt gggttcttcg gatgcagtac
    18541 aagtgggaga ctgggcgatc gccgtgggaa atccgttagg atttgataat actgtgactt
    18601 tgggaattgt cagcactctc aaacgttcta gcgcccaagt gggaattgct gacaaacgtt
    18661 tagactttat tcaaaccgat gcggcgatta atcctggtaa ctccgggggt ccgttattga
    18721 atgaacgtgg cgaagtgatt gggattaata cggctattcg tcctgatgct atgggtattg
    18781 gctttgcgat tcctattgat aaggctaagg cgatcgcctt gaaattgcaa cgtgacggta
    18841 aagttattca cccctattta ggtgtgcaaa tggtgacttt aacgcctgaa ctcgcaaagc
    18901 agaataatac tgatcccaat tctccgattc aaataccaga aattaatggt gttttggtga
    18961 tgcgagttgt acctgattcg cctgctgcat ctgctggtat gcgtcgtggg gatgtgattg
    19021 tgcaaattga tcgcgaacct gtcactagtg ctgataagtt acaaaatctt gtggaaaata
    19081 gtcgtctcgg tcagttgttg caggtgaaag tccaacgcgg taatcagata cagttgcttt
    19141 caatccgcac tgctgagtta caagatccgg cttaggaatc agcccaggtg ggcatctctg
    19201 ccctccccca caagatggat aatttatttc tgggaaatct cagtaggtgg ttgaaattaa
    19261 atataaattg tagtgtggtc atcttgcctt cgcactacgg ttatctacga attaattaca
    19321 cctacctact tatagcggtt ctcatcttta taggtactac aagaatgatt cgaccacaga
    19381 taaacacaga taaacacaga taaacacaga taaattctga cctcagcaga ctaggaaacg
    19441 ctttattcgc gaaatttttg tagatttaaa ttggcaatga ctgcaaaatt gactcatgct
    19501 ttatagaaga tttggccgca cagaattaca aatgcctgtt ttttcttgtg gcgggatgag
    19561 ataccagttc aaatggcaag atgtccctca ttcggaaatt cctgcggata atcaggcaaa
    19621 tctagaagcg actattcgcc gatctctaga cgtgggaatt aatcacattg aaactgcccg
    19681 tggatatggt acatcagaaa tgcaattggg gcaaatattg cccaagtttc cccgtgagaa
    19741 attaattgtg cagacaaaag tcagcccagt cgcagatgct cagaaatttc gcaaaacatt
    19801 tgaacaatca ctgcgaaatc tccagttaga ttatgttgat ttactgggca tacatggcat
    19861 taatcatgct gaatctttcg agaatactat gcgtcccggt ggctgtttgg atgtggcgcg
    19921 acagttgcaa aaagagggga aagtaaaatt tattggcttt tctactcatg gatctaaaga
    19981 ggtaattatt caggcaatta atactaacca atttgattat gtcaacctgc attggtacta
    20041 cattaatcaa tggaattggg aggcaattac agccgctaac aatcatgata tgggggtatt
    20101 tattattagt ccttcagata aaggagggat gctatataaa ccaccgcaaa agttagttaa
    20161 tatttgcgct cctttaagtc cgatggtgtt taatgattta ttttgtctga gtcatcaaaa
    20221 tgtacatact ttgagtgtgg gtgcatccag acctgaagat tttgatgaac atctcaaaac
    20281 tttagaatat ttagaccaag cggaggaaat tctgccgcca attttgagta aattagaaca
    20341 agaagcgatc gcctctttag gagaagattg ggtcaagact tgggagacta atttacctac
    20401 ctgggaaaaa acaccgggag aagtaaatat tcgggtaatt ttgtggttgt tgaatctggc
    20461 gatcgcctac gatttagtag aatacgccaa aatgcgctac aacttattgg gtcatggtag
    20521 tcactggttc ccaggtaaca aagcggacaa actagatgaa cttgacctga gtctatctct
    20581 cgccaatagt ccccacgccg aaaaaattcc gcaattatta gcacaagccc atcaaatgtt
    20641 agccggtgct gaagttaagc ggttatcaaa aacttagtca ttagtcatta gtcattggtc
    20701 attggtcatt agtcattagt cattagtcat tagtcatttc cctactccct actccccact
    20761 ccccactgta taaagataat tccgtaccga gaagatgagg aaacgccata acacttttaa
    20821 aatggtgaat ataaactaac tctaattttt cttattttta aggacttcct cctgtagtaa
    20881 aacttatggc attttcttct attgtgcgtg ctttagcgcg atcgcctctc agt
//
