LOCUS       NZ_CP084681            10984 bp    DNA     linear   CON 30-APR-2024
DEFINITION  Streptomyces inhibens strain NRRL30439 chromosome, complete genome.
ACCESSION   NZ_CP084681
VERSION     NZ_CP084681.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces inhibens
  ORGANISM  Streptomyces inhibens
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 9696490)
  AUTHORS   Barry,C.P., Gillane,R., Talbo,G.H., Plan,M., Palfreyman,R.,
            Haber-Stuk,A.K., Power,J., Nielsen,L.K. and Marcellin,E.
  TITLE     Multi-omic characterisation of Streptomyces hygroscopicus NRRL
            30439: detailed assessment of its secondary metabolic potential
  JOURNAL   Mol Omics 18 (3), 226-236 (2022)
   PUBMED   34989730
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9696490)
  AUTHORS   Barry,C.P., Palfreyman,R. and Marcellin,E.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-OCT-2021) EMS, Australian Institute for Bioengineering
            and Nanotechnology, Cnr College Rd & Cooper Rd, Brisbane, Queensland
            4103, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. APRIL-2018
            Assembly Name                     :: AIBN_shyg
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 239.0x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022221585.1-RS_2024_04_30
            Annotation Date                   :: 04/30/2024 04:58:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,775
            CDSs (total)                      :: 8,682
            Genes (coding)                    :: 8,305
            CDSs (with protein)               :: 8,305
            Genes (RNA)                       :: 93
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 158 of 377
            Pseudo Genes (incomplete)         :: 310 of 377
            Pseudo Genes (internal stop)      :: 38 of 377
            Pseudo Genes (multiple problems)  :: 116 of 377
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 23:32:03
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 8901122
            Orig. end                         :: 8912106
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP084681.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Australian Institute for
            Bioengineering and Nanotechnology, Building 75,Cnr College Rd &
            Cooper Rd, Brisbane City QLD 4072.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10984
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="27"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10984
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10984
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5984](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5984)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            313..540
                     /locus_tag="KI385_RS39950"
                     /old_locus_tag="KI385_40030"
     CDS             313..540
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608014.1"
                     /locus_tag="KI385_RS39950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KI385_40030"
                     /product="hypothetical protein"
                     /protein_id="WP_128503932.1"
                     /transl_table=11
                     /translation="MTEHPPVIVHPPTPSGGRRVTVRGQIVGLAHGRGDVAEFLRRAGV
                     AGPAEDIELDDPRLVEWRGGSLNDWPMPPA"
     gene            783..959
                     /locus_tag="KI385_RS39955"
                     /old_locus_tag="KI385_40035"
     CDS             783..959
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608013.1"
                     /locus_tag="KI385_RS39955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KI385_40035"
                     /product="hypothetical protein"
                     /protein_id="WP_238576892.1"
                     /transl_table=11
                     /translation="MSDGDDSGGYGDDSGVTGSPTRNSRSESLPNWSVLRRAMRWETLV
                     LVVVLVILAAAMS"
     gene            1150..2685
                     /locus_tag="KI385_RS39960"
                     /old_locus_tag="KI385_40040"
     CDS             1150..2685
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018092055.1"
                     /locus_tag="KI385_RS39960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KI385_40040"
                     /product="AlkA N-terminal domain-containing protein"
                     /protein_id="WP_238576893.1"
                     /transl_table=11
                     /translation="MTNQDSRYEAVRSRDARFDGAFFFAVSTTGIYCRPSCPATTPRRR
                     NVTFFPTSAAAQGNGFRACRRCRPDAVPGSAEWNARADVVGRAMRLIGDGVVDREGVSG
                     LADRLGYSARQVQRQLNAEVGAGPVALARAQRAHTARVLLQTTGLRAAEIAFAAGFASV
                     RQFNDTIKEIYALTPSELRAARPGKGARFGPVAPPATPGVLPLRLAFRGPYAAREIFDH
                     LQRRALTGIEDVTGEPGARTYRRTLRLPHGPGIAEVDEPPGDGWLDCRLHLTELRDLTT
                     AGQRVRRLFDLDADPYAVAERLGADPVLARLVAERPGLRSPGAPAPHELAVRAVLGQQV
                     SVAAGRTLGGALVTAYGEPLPEPSGALTHLFPRADDLAQASLAELGMPESRRTTLRTLS
                     AALAGGAVTLDAGADRDETERALLGLRGIGPWTAGYIRMRALGDPDVLLSGDAAVLAGV
                     RRAGASTAVLKAHAQAWRPWRSYAMHHFWNVSARNASAPRTTTRPHTTERTGP"
     gene            2682..3194
                     /locus_tag="KI385_RS39965"
                     /old_locus_tag="KI385_40045"
     CDS             2682..3194
                     /EC_number="2.1.1.63"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608011.1"
                     /locus_tag="KI385_RS39965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KI385_40045"
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="WP_128503936.1"
                     /transl_table=11
                     /translation="MTLYATLDSPLGELLLVGEESATAPGGTALASLSLPGQKGGATVQ
                     DGWTYDPAAFTEIVQQLHAYFDGKLTRFSIEYASTRGTDFQQRVWQALEAIPYGTTTTY
                     GRIAADIGAARGAVRAIGTAIGANPLLVVRPCHRVIAADGSLSGYAGGPERKRQLLGLE
                     GAVPDAA"
     gene            3260..3970
                     /locus_tag="KI385_RS39970"
                     /old_locus_tag="KI385_40050"
     CDS             3260..3970
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020117149.1"
                     /locus_tag="KI385_RS39970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KI385_40050"
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="WP_238576894.1"
                     /transl_table=11
                     /translation="MTTTTTKRVAAADWTALAGELDTVGCALTPRLLAPADCRALIALY
                     DEAERFRSTIDMARHRFGSGQYRYFADPLPEPVQALREAFYPHLLKIARDWADRLGRPA
                     PWPDTLGEWLTLCHKAGQTKPSQILLRYGAGDWNALHRDLFGEMVFPLQVVVGLDEQGT
                     DYTGGEFLLVEQRPRAQSRGTTTVLPQGHGLIFTTHDRPVRSTRGWSAGPVRHGVSTVR
                     SGHRHSLGLVFHEA"
     gene            complement(4016..4780)
                     /locus_tag="KI385_RS39975"
                     /old_locus_tag="KI385_40055"
     CDS             complement(4016..4780)
                     /NRPS_PKS="Domain: PKS_KR (7-181). E-value: 1.9e-10. Score:
                     32.9. Matches aSDomain:
                     nrpspksdomains_KI385_RS39975_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608009.1"
                     /locus_tag="KI385_RS39975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KI385_40055"
                     /product="SDR family oxidoreductase"
                     /protein_id="WP_238576895.1"
                     /transl_table=11
                     /translation="MGDLKGKTALVTGSSRGIGRAIAQRLAQDGALVAVHYGSNDLAAK
                     ETAEGIGSAGGQAFIIGAELGIPGDAEALFAAFDAELAAYGAEPGLDILVNNAALNFPG
                     HLDDVTPEEFDRTIAVNTKAPFFIAQHGLRRMRDGGRIINISSAVTSTALPSQIAYSVS
                     KGGMDTLTLTLAKEVGERGVTVNTIAPGWVETEVTAARRATPEGRAALAAYSVFNRIGQ
                     PADIADVAAFLASDDSRWITGQRFDVTGGSML"
     aSDomain        complement(4238..4759)
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_KI385_RS39975_PKS_KR.1"
                     /evalue="1.90E-10"
                     /label="KI385_RS39975_PKS_KR.1"
                     /locus_tag="KI385_RS39975"
                     /protein_end="181"
                     /protein_start="7"
                     /score="32.9"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="TALVTGSSRGIGRAIAQRLAQDGALVAVHYGSNDLAAKETAEGIG
                     SAGGQAFIIGAELGIPGDAEALFAAFDAELAAYGAEPGLDILVNNAALNFPGHLDDVTP
                     EEFDRTIAVNTKAPFFIAQHGLRRMRDGGRIINISSAVTSTALPSQIAYSVSKGGMDTL
                     TLTLAKEVGER"
     CDS_motif       complement(4688..4753)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_KI385_RS39975_0001"
                     /evalue="3.70E-05"
                     /label="PKSI-KR_m1"
                     /locus_tag="KI385_RS39975"
                     /protein_end="31"
                     /protein_start="9"
                     /score="16.4"
                     /tool="antismash"
                     /translation="LVTGSSRGIGRAIAQRLAQDGA"
     gene            complement(5001..5984)
                     /locus_tag="KI385_RS39980"
                     /old_locus_tag="KI385_40060"
     CDS             complement(5001..5984)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608008.1"
                     /locus_tag="KI385_RS39980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KI385_40060"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_281066951.1"
                     /sec_met_domain="AfsA (E-value: 1.4e-17, bitscore: 62.5,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDTMHVSGTVQPRAPHQLRLSRSQRSTPRTRPSARVPREFVHRP
                     LVDDILVTSWRRRDDSHFSLTAQWPHDHGYFIPNHGRHHLILTGETIRQAGLLLCHTEL
                     GVPVGHHFILGDLVYTTHPEHLAVGSGPTPLTIDVTCSKMRMRAGTMTSGRFDMTIRKG
                     GRIVATGHSNVTVASPAVYRRIRGERLTARRTLGPLPTSVPPRLTGSLSDSDVLLAPTD
                     RPDRWLLRIAPGHAAVVNPANDHVPGMVLLDAAQQAARVLTAPDAFVPYAFGTEFRRYA
                     EHATPCVIEARRVPSALPCTTTVQVTGSQDGEPVFVSTLTALDPRP"
     misc_feature    complement(6046..6059)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 15.3"
                     /tool="antismash"
     misc_feature    complement(6054..6074)
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 18.75"
                     /tool="antismash"
     gene            6159..6857
                     /locus_tag="KI385_RS39985"
                     /old_locus_tag="KI385_40065"
     CDS             6159..6857
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018092050.1"
                     /locus_tag="KI385_RS39985"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KI385_40065"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_238576897.1"
                     /transl_table=11
                     /translation="MAQQDRAIRTRRVILEAAASVFDDQGYDRATIAEVLERAGVTKGA
                     LYFHFASKEQLALAVLEEHVVDIAVEPQKIKLQEFVDAGQVLAFRLRSDPIQRGAARLA
                     VEQGSNRLDRKRSMLSWTRFVEGLLTEAKERGEVLESIVVHETAELFVGAFAGLQMMSH
                     ALTNQADLCYRLTVFFEHTLPSVAVPAILAKLNLDPERGAKLDTALQGKVLAEAEADAD
                     VNADTDVALK"
     misc_feature    complement(6902..6916)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.2"
                     /tool="antismash"
     misc_feature    complement(6916..6933)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 15.01"
                     /tool="antismash"
     misc_feature    6920..6938
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: weak, score: 20.41"
                     /tool="antismash"
     misc_feature    complement(6921..6939)
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: weak, score: 20.03"
                     /tool="antismash"
     misc_feature    complement(6925..6934)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 14.58"
                     /tool="antismash"
     misc_feature    complement(7053..7068)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 16.26"
                     /tool="antismash"
     misc_feature    7054..7067
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 18.18"
                     /tool="antismash"
     misc_feature    7114..7127
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: medium, score: 21.19"
                     /tool="antismash"
     gene            7329..9725
                     /locus_tag="KI385_RS39990"
                     /old_locus_tag="KI385_40070"
     CDS             7329..9725
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1005: Drug
                     resistance transporter, EmrB/QacA"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016574653.1"
                     /locus_tag="KI385_RS39990"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KI385_40070"
                     /product="MFS transporter"
                     /protein_id="WP_238576898.1"
                     /transl_table=11
                     /translation="MTRTTTDQLPGRAATTAAAPTVPDPAVVGRRRTRGIVPVLAFSGI
                     VVAVMQTLLVPVIKDLPVLLNTAPSNATWVMTATLLAGAVSTPIMGRLGDLYGKRRMLL
                     TSLAVMVVGSLICGFTSDLLTMIVGRALQGFAMGAIPLGIGIMRDELPRERLGSAMGLM
                     SSSIGVGGGLALPAAALVAQHANWHTLFFGAAGLGVLSMLLTLLVVPETAVRAPGRFDV
                     VGAIGLSAGLVALLLPITKGSDWGWGSPTTLGLFGIAALILLLWGVMELRIADPLVDLR
                     TSARREVLLTNLASITVGVAFYAVSLVLPQLLQLPKSTGYGLGQSMVVAGLCVAPLGLT
                     MMLVAPLYARIAARRGPKASLLLGMLVIALGYGAGIGLMSAPWQTVIIAVVLGAGIGLA
                     YSSLPALIIGAVDPSETGAANGLNTLMRSIGTSVSSAVIGMVLAHMSTSAGPVTVPTMA
                     GFRVAFLIATASVLVGAALASFLPSQRKASRPTLVAQSTDGAEEADGAVGVPGARGGGD
                     PEGVERLAAAATEPTVRTLPERAERGGFRGRVVDASGAPVPGASVTLIDRQGRQAGVTT
                     AGADGRYALGAPAAGTYVLTGAAPGHAPYAASATYRGEGAPAELDLILAGSGRLGGILL
                     GGREGAPLAGGSVVVTDAGGDVVTRTTSGAEGSWDVGRLSPGSYTLVLSAPGHQPQARA
                     VELSGAAPERQDARLQPTATVHGTVRGPNGRPLADAAVTLVEDGTVTGHIVTGADGAFA
                     FSDLSGSHYTLTAAGYPPHGAPISLTGGADEILDLDLAQPPAATG"
     gene            complement(9791..10483)
                     /locus_tag="KI385_RS39995"
                     /old_locus_tag="KI385_40075"
     CDS             complement(9791..10483)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1071: GntR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016570252.1"
                     /locus_tag="KI385_RS39995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KI385_40075"
                     /product="FCD domain-containing protein"
                     /protein_id="WP_238576899.1"
                     /transl_table=11
                     /translation="MSLDALRPSPLVEQAAQRLRDQITGGHWPVGTKLPGETTLAKTLG
                     VGRSTVREAVRALAGAGLVQPRQGAGVFVIATEPAEDWPTRLRRAAVADVYEVRAMVEV
                     QAARLAAARRTDADVAAMRSALEGRRTAAAGSDDAAFVDADIALHATVVDAAHNPVLTD
                     LFTEFAPALRQGLIDLVELLGLRGQDPQHGDARHAALIEAVADGDGEAASRTLRAEFDE
                     TARQLAKD"
ORIGIN
        1 ttgcggccgc tcggccggtc ggcgaagtag cgggcggtgt ccttcgacag ccgggcatcc
       61 gccagtgccg cacgcgcctc cgcccatccc gtgacgaccc atgtcgtccg gccgttgccc
      121 gtcctgatcc gctgtacggg cccgcgcgcc cgcaaggcgg cgagcagcgg atacgggtcc
      181 cgggagaact cggcagagtc catggggtgg tgtgccactc cgccagccta gcgagtgctt
      241 gcggcctcaa ggaccgacgc tcgcggcggc tgtcgacgtg cgcgagcgcg gagcccggcg
      301 cacgctggag gcatgaccga gcacccgcct gtcatcgtgc acccgccgac gccgtccggt
      361 ggtcgacggg tgaccgtgcg tgggcagatc gtcggcctgg cccacggccg cggtgatgtc
      421 gccgaattcc tccgccgcgc gggagtcgcc ggcccggcag aagacatcga gctcgacgac
      481 ccgaggctgg tcgaatggcg cggtggctca ctgaatgact ggccgatgcc gccggcgtga
      541 ccgctcaccc cgtatgacgg ccggactctc cgcggccggg cgcctgctgt gtgcggttcc
      601 gctgcacaga gcggcccgcg ggagccaccc gcgtgcggtg acggctggat caggtatgaa
      661 tgggggaggc tgacacggcg accggaacca cgcacggcgg ccgagacact tcacacggca
      721 ggcgcgacag agggggcgga aatgccggag agtccggaga tgccggagac cgggggcacg
      781 ccgtgagcga cggcgatgac agtggcggct acggcgacga cagcggcgtt accggaagcc
      841 cgacaaggaa cagccggtcc gagagcctcc cgaactggag cgtcctgcgg cgggccatgc
      901 gctgggagac ccttgtgctg gtcgtggtcc tggtgatcct cgcggcggcg atgtcgtaac
      961 gcccagccct cgccggtacg acggcctgcc cgaactgccc tcctgctgac gggtgttgtc
     1021 ggaggggtgg gggtggggcg tccccagggg acgccgggcg taccggggta cgcgtcccgg
     1081 actcgtgtcc cggactcgcc gtccgatttc cgccagcgtc gccgtccggc ctgctgcaca
     1141 gtggaccttg tgacgaacca ggacagcaga tacgaggcgg tgcgcagccg ggacgccagg
     1201 tttgacggcg cgttcttctt cgccgtgtcc acgaccggga tctactgccg gccgagctgc
     1261 cccgccacca ctccgcgacg ccggaatgtc accttcttcc cgacctccgc ggccgctcag
     1321 ggcaacggtt tccgtgcctg ccgccgctgc cggcccgatg ccgtgccagg gtccgccgag
     1381 tggaatgccc gcgccgatgt cgtcggccgg gccatgcggc tgatcggcga tggcgtggtc
     1441 gaccgggaag gagtctccgg gctggccgac cggctcggct acagcgcccg gcaggtgcag
     1501 cggcagctga acgccgaggt gggcgccgga cccgtggcac tggcgcgggc acagcgtgcc
     1561 cacaccgccc gggtcctgct gcagaccacc ggcctgcggg cggccgagat cgccttcgcg
     1621 gccggcttcg ccagcgtgcg gcagttcaac gacaccatca aggagatcta cgcgctcacc
     1681 ccgagcgagc tgcgcgccgc ccgccccggg aagggagccc gattcggacc ggtggccccg
     1741 cccgccacgc ccggcgtcct gccgctgcgg ctggccttcc gcggcccgta cgccgcgcgg
     1801 gagatcttcg accacctcca gcggcgtgcg ctcaccggta tcgaggacgt caccggggaa
     1861 ccgggcgccc gcacctaccg gcgcaccctg cgactgccgc acggacccgg catcgccgag
     1921 gtcgacgagc cgcccggcga cggctggctg gactgccggc tgcacctcac cgagctgcgc
     1981 gatctcacca ccgccggcca gcgggtgcgc cgcctcttcg acctggacgc cgatccgtac
     2041 gcggtcgccg aacggctcgg tgcggacccg gtactcgcgc ggctggtggc cgagcgcccc
     2101 gggctgcgct cgccgggcgc acccgccccg cacgagctcg ccgtccgcgc cgtactcggc
     2161 cagcaggtat cggtggccgc cggccgcacg ctcggcggcg ccctggtcac cgcctacgga
     2221 gagccgctgc cggagccgtc cggcgcgctc acccatctct ttcctcgcgc tgatgacctg
     2281 gcgcaggcct cgctagcgga actcggcatg cccgagtcgc ggcgtacgac cctgcgcacc
     2341 ctcagcgccg cactcgccgg cggggcagtc accttggacg ccggagccga ccgtgacgag
     2401 accgagcgcg cgctcctcgg cctgcgcggc atcggcccgt ggaccgccgg ctacatccgg
     2461 atgcgagcgc tcggggaccc cgatgtgctg ctgtccggcg atgcggccgt actggccggt
     2521 gtgcgtcggg ccggggcctc gacggccgtg ctgaaggcgc acgcgcaggc ctggcgcccc
     2581 tggcgctcgt acgcgatgca ccacttctgg aacgtgtcgg cccgcaacgc ctcggccccc
     2641 cgcaccacca cccgccctca caccaccgaa agaacaggcc catgacgctc tacgccaccc
     2701 tcgacagccc gttgggtgaa cttctgctgg tcggcgagga gtcggccacc gcgccgggcg
     2761 gtaccgcact cgcctcgctg tccctgcccg gtcagaaggg cggggcgacc gtccaggacg
     2821 gctggacgta cgacccggcc gcgttcaccg agatcgtcca gcagctccat gcgtacttcg
     2881 acggcaagct gacccgcttc agcatcgagt acgcgagcac ccggggcacg gacttccagc
     2941 agcgcgtgtg gcaggccctg gaggccattc cgtacggcac cacgaccacg tacgggcgga
     3001 tcgccgccga catcggtgcg gcgcgcggtg cggtgcgcgc gatcggaacg gcgatcggcg
     3061 ccaacccgct gctcgtcgtc cggccctgcc accgtgtgat cgccgcggac gggtcgctct
     3121 cgggttacgc gggcgggccg gaacgcaaac ggcagctcct cggccttgag ggcgccgtac
     3181 cggacgcggc ctgatcccgc ccggcgccgc ctccgatccc cgtgagccgc ccccgcccga
     3241 cgcactgctg gagcacgtca tgaccaccac gaccaccaag cgcgtcgcgg ccgccgactg
     3301 gacggctctg gccggggaac tcgacaccgt gggctgcgcg ttgaccccac ggctgctcgc
     3361 cccggcggac tgccgtgcgc tgatcgcgct gtacgacgag gccgagcgct tccgttcgac
     3421 catcgacatg gcacggcacc gcttcggctc gggccagtac cgctacttcg ccgatccgct
     3481 gcccgagccg gtccaggccc tgcgcgaggc gttctacccg catctgctga agatcgcccg
     3541 cgactgggcg gaccggctcg gcaggccggc gccctggccg gacacgctgg gggagtggct
     3601 caccctgtgc cacaaagccg ggcagaccaa gccctcccag attctgctgc gttacggggc
     3661 gggcgactgg aacgcgttgc accgcgacct cttcggggag atggtcttcc ccctccaggt
     3721 cgtcgtcggc ctggacgagc agggcaccga ctacacgggc ggggaattcc tcctcgtcga
     3781 gcagcggccg cgcgcccagt cccgcggcac caccaccgtc ctgccacagg gccacggcct
     3841 gatcttcacc acccacgacc gccccgtacg ctccacccgc ggctggtcgg cagggcccgt
     3901 acgccacggt gtcagcaccg tccgctcggg ccaccggcac tccctgggcc tggtcttcca
     3961 cgaggcctga agacgccctg ccaccgaccc gggcccgggg aagtgcgcaa cgggctcaga
     4021 gcatggagcc gccggtgaca tcgaagcgct ggccggtgat ccagcgggag tcgtccgacg
     4081 cgaggaacgc ggcgacgtcc gcgatgtccg cgggctgccc gatccggttg aacaccgaat
     4141 aggccgccag cgcggcccgc ccctcgggcg tcgccctccg cgccgcggtc acctcggtct
     4201 cgacccagcc gggtgcgatg gtgttcaccg tgaccccgcg ctcgccgacc tccttggcga
     4261 gggtgagcgt gagggtgtcc atcccgccct tggagacgct gtaggcgatc tgcgacggga
     4321 gcgccgtact cgtcaccgcc gacgagatgt tgatgatgcg tccgccgtca cgcatccggc
     4381 gcagaccgtg ctgggcgatg aagaacgggg ccttggtgtt gaccgcgatg gtgcggtcga
     4441 actcctcggg agtcacgtca tccaggtgcc cgggaaagtt gagcgctgcg ttgttgacga
     4501 gaatgtcgag gccgggttcc gcgccgtacg cggcgagttc ggcgtcgaag gcggcgaaga
     4561 gggcttcggc atcaccggga atccccagtt ccgcgccgat tatgaaggcc tggccgccgg
     4621 cgcttccgat gccctcggcc gtttccttgg ccgcgaggtc attgctgccg tagtggacgg
     4681 cgaccagcgc gccgtcctgg gcgaggcgct gggcgatggc tcggccgatg ccccggctgg
     4741 atcccgtcac cagcgcggtc ttccctttga ggtcacccat gatgtgtagg ccttccgttt
     4801 gcccgtggtg tgcgtgcttc cgccgtgatc ggtggcgatg tcttcaggcc ggccggcgag
     4861 cacacggctg tccgggccag gttcctgagc cggctgtcgg cggggtggta cgagtgctcc
     4921 gtaccgcgcg acttccggtg aagtgtgggc tgttgctgcc cgtcggggtg gtgcccagga
     4981 cgcatccgtg agctgttcgc tcaggggcgt gggtccagcg ccgtcagtgt ggagacgaac
     5041 accggctcgc cgtcctggga gccggtgacc tggaccgtcg tggtgcaggg cagcgccgac
     5101 ggcacccggc gggcctcgat cacgcacgga gtggcgtgct cggcgtagcg gcggaattcg
     5161 gtaccgaagg cgtacggcac aaaggcatcg ggcgcggtca ggacacgggc ggcctgctgt
     5221 gccgcgtcga gcagcaccat gccgggaacg tggtcgttcg ccgggttcac gaccgcggcg
     5281 tggccgggcg cgatgcgcag cagccagcgg tcgggccggt cggtgggggc gagcaggaca
     5341 tcgctgtcgg agaggctgcc ggtcagccgg ggcggaacgg acgtgggcag cgggccgagg
     5401 gtgcgccgtg cggtcagccg ctcgccgcgg atccggcggt agacggcggg agaggcgacg
     5461 gtgacgttgg agtgacccgt cgccacgatg cgtccaccct tacggatggt catgtcgaac
     5521 cggccgctgg tcatggtgcc ggcccgcatc cgcatcttgc tgcatgtgac gtcgatggtc
     5581 agcggcgtgg gcccgctgcc aacggccaga tgctcggggt gcgttgtgta gacgaggtcg
     5641 ccgaggatga agtggtggcc gaccggtacg ccgagttcgg tgtggcagag cagcagcccc
     5701 gcctggcgaa tggtctcgcc ggtgagaatg agatggtggc ggccgtggtt ggggatgaag
     5761 tacccatgat cgtgcggcca ctgggcggtc agcgagaagt gggagtcgtc ccgcctgcgc
     5821 catgaggtga ccagaatgtc gtcgacgagg ggacggtgca cgaactcgcg ggggacacgg
     5881 gcgctgggac gggtccgcgg tgtggagcgc tggctccgac tcaggcgcag ttgatgtgga
     5941 gccctgggtt gtaccgtgcc gctgacgtgc atcgtgtcgc tcatgactgc ccccaggcgt
     6001 ctctacggga tgaggtccat cgctgttaag atacgtacca tccggtttta ttttcaatgc
     6061 caggtcgacg aaaaggagta agtagctgat ctagcggcga cttgagctgt agctaactgt
     6121 tgcggaactt gaaaacttcg aaagacagga ggcgtcctgt ggcacaacag gaccgcgcga
     6181 tccggacccg gcgagtgatc ttggaggcgg ccgcgtcggt tttcgacgat caaggctacg
     6241 accgcgcgac catcgccgaa gttctggaac gcgccggcgt gacgaagggc gccttgtact
     6301 tccacttcgc ctccaaggag cagctcgcgc tcgcggtgct cgaggagcat gtggtcgaca
     6361 ttgcggtgga accgcagaag atcaagctgc aggaattcgt cgacgcgggt caggtgctgg
     6421 cgttccggct gcgcagtgat ccgatccagc gcggtgcggc gcggctggcg gtcgagcagg
     6481 gctccaaccg ccttgatcgt aagcggtcga tgctgtcgtg gacccggttc gtcgagggcc
     6541 tgctgaccga ggcgaaagag cgcggcgaag tcctggagag cattgtcgta cacgagaccg
     6601 ccgagctgtt tgtcggcgca ttcgccgggc tccagatgat gtcccacgcg ctgaccaatc
     6661 aggcggatct ctgttacagg ttgacggtct tcttcgagca caccctcccc agcgttgcgg
     6721 tcccggccat cctcgccaag ctcaacctgg acccggagcg tggtgcgaaa ctcgataccg
     6781 ctctgcaggg gaaggtgctg gcggaggccg aggccgacgc cgatgtgaat gccgacaccg
     6841 atgttgctct gaagtgagac tcgccgggtc ggagtggctg agcccgaact gggtgatgcg
     6901 gaatatttgg gatattaaac gctgtaaaaa cataccgcgc agacggtgtt gatcgtagag
     6961 aggtgcacgg gtcacgtcgt gtacggcaga ggcgtcgcgg aggatccgcg gcgcctctcg
     7021 acggttgacg gggtgcggcg gtcggcagta agcgtgaggc agatcaccac cgttgttgtg
     7081 ggatgccgcc ctgcgcggcg gaatagggct ccgagaaaaa acgttcggca tgcacgtatc
     7141 aacggctcgg ctgtgccgac gtccgtagac gacgccccaa gacgacgccc caagacgacg
     7201 ccccaagacg acgccccaag acgacgcccg tagaagacgc ccgagatccc ccacgcctgc
     7261 cgctcgccgc tgctctgccg gccccccgac gacacccgtt cacctctgcc gccctggagg
     7321 ccgcacgcat gacccggacg acgacggacc agctacccgg aagggcggcg accaccgccg
     7381 ccgcgccaac ggtgcccgac ccggctgtgg tcgggcgccg tagaacccgc ggcatcgttc
     7441 cggttctcgc cttctccggc atcgtcgtcg cggtgatgca gacgctgctc gtgccggtca
     7501 tcaaggacct gccggtgctg ctcaacacgg cgcccagcaa tgccacttgg gtgatgacgg
     7561 ccaccctcct cgcgggtgcc gtctcgacgc ccatcatggg gcgcctcggc gacctctacg
     7621 gcaagcgcag gatgctgctg accagcctgg cggtcatggt cgtcggctcg ctgatctgcg
     7681 gattcaccag cgacctgctc accatgatcg tcggccgggc gctacagggc ttcgccatgg
     7741 gagccatccc gctcggcatc ggcatcatgc gcgacgagct gccgcgcgaa cggctcgggt
     7801 cggccatggg gctgatgagc tcctccatcg gcgtcggcgg cgggctcgcg ctgccggccg
     7861 cggccctggt ggcccaacac gccaactggc acaccctgtt cttcggcgcc gccgggctcg
     7921 gtgtgctgtc gatgctgctc accctcctcg tcgtaccgga gaccgcggta cgcgccccgg
     7981 gtcgcttcga cgtggtgggc gccatcgggc tgtccgccgg gctggtggcg ctgctgctgc
     8041 ccatcaccaa gggcagcgac tggggctggg gttcacccac caccctcggc ctgttcggca
     8101 tcgcggccct gatcctgctg ctgtggggcg tgatggagct gcgcatcgcc gatccgctgg
     8161 tcgatctgcg gacctccgcc cgccgcgagg tgctgctgac caacctcgcg tcgatcaccg
     8221 tcggagtggc gttctacgcg gtctcgctgg tgctgccgca actgctgcag ctgcccaagt
     8281 cgaccggcta cggcctgggc cagtcgatgg tggtggccgg tctgtgcgtg gcgccgctgg
     8341 gcctgacgat gatgttggtc gccccgctgt acgcgcggat cgcggcacgg cgcggcccca
     8401 aggcgtcgct gctgctgggc atgctggtca tcgcgctcgg ctacggcgcc ggcatcggcc
     8461 tgatgagcgc cccttggcag accgtcatca tcgcggtggt cctcggtgcc ggtatcggtc
     8521 tcgcctactc gtcgttgccc gcattgatca tcggcgccgt cgacccgtcc gagaccggcg
     8581 ctgccaacgg tctgaacacc ctgatgcgct cgatcggcac ctcggtttcc agcgccgtga
     8641 tcggcatggt gctggcccat atgtccacga gcgcgggccc ggtcacggtg cccacgatgg
     8701 ccggtttccg ggtcgccttc ctgatcgcca cggcctcggt cctcgtcggc gccgccctgg
     8761 cgtccttcct gccgtcgcag cggaaggcat cccgtccgac cctggtcgcg cagagcacgg
     8821 acggtgcgga ggaggcggac ggcgcggtcg gcgtgcccgg cgcacgaggc gggggagacc
     8881 cggagggcgt ggagcggctc gcggcggcgg ccactgagcc gaccgtgcgc acactgcccg
     8941 aacgggccga acgcggcgga ttccggggcc gcgtcgtgga cgcgtccggc gcgccggtcc
     9001 cgggcgcgag cgtcaccctg atcgaccggc aggggcgcca ggccggcgtc accacggccg
     9061 gcgccgacgg ccgctatgcg ctcggcgctc cggctgccgg cacctacgtc ctcacgggcg
     9121 ccgcgcccgg ccacgccccg tacgccgctt ccgccaccta ccgcggcgag ggcgccccgg
     9181 ccgagctcga tctgatactc gccggctccg ggcggctcgg cggcatcctg ctcggcggca
     9241 gggaaggcgc cccgctggcc ggcgggagcg tcgtggtcac cgatgccggc ggcgacgtgg
     9301 tgacccgtac gacatcgggc gcagagggga gttgggacgt cggccggctg tcgcccggca
     9361 gctacaccct ggtcctcagc gcccccggcc accagccgca ggcccgtgcc gtcgaactgt
     9421 ccggcgccgc gccggagcgg caggacgccc ggctgcaacc gaccgcgacc gtccacggca
     9481 cggtacgagg tccgaacggc cgcccactgg ccgatgccgc cgtcaccctc gtcgaggacg
     9541 gcacggtcac cgggcacatc gtcaccggcg cggacggcgc cttcgccttc tccgacctga
     9601 gcggcagcca ctacaccctc acagcggccg gctacccgcc ccacggcgcc ccgatatccc
     9661 tgacgggcgg cgccgacgag atcctcgacc tggacctcgc ccagccgccc gccgccacgg
     9721 gctgacgtac ggcccgctcc ctcccccgta ggtcagcggg ggagggaagc ggggcgcggc
     9781 gcggctgccc tcagtccttc gccaactgcc ttgccgtctc gtcgaattcg gcccgtagtg
     9841 tccggctcgc cgcctccccg tccccgtcgg cgacggcctc gatcagggcc gcatgacggg
     9901 catcgccgtg ctgggggtcc tggccgcgaa gtcccagcag ctccacgagg tcgatcaggc
     9961 cctgccgcag cgccggcgcg aactcggtga acagatcggt cagtacggga ttgtgcgcgg
    10021 cgtcgaccac ggtcgcgtgc agcgcgatat ccgcgtccac gaacgccgcg tcgtcgcttc
    10081 cggcagcggc cgtgcgccgt ccctccagag cggaccgcat ggccgcgaca tcggcgtcgg
    10141 tacgccgggc ggcggccaga cgggcggcct gtacctcgac catcgcgcgg acctcgtaga
    10201 catcggcgac ggccgcgcgg cgcagacggg tgggccagtc ctcggcgggc tcggtggcga
    10261 tcacgaagac gccggcgccc tggcgtggct gtaccaaccc cgcgccggcc agggcccgta
    10321 cggcttcgcg cacggtcgag cggccgacgc cgagcgtctt ggccagggtg gtttcaccgg
    10381 ggagcttggt gcccaccggc cagtgcccgc cggtgatctg gtcgcgcagt cgctgggcgg
    10441 cctgttcgac cagggggctg gggcggagcg cgtcgagcga catcccgcgt cacctctcgg
    10501 ttcaggtgct cggtcccggg cgcttatgtc tcgggtgccg agatctcagg ttctcagctt
    10561 gtctgaggag ttggtccatg gctagggtac ccgccatgaa gctgcgtggt ctccttctcg
    10621 ggcgccgtgg cggggcctga cgcgaccggc accccgccgc ggggttcggt gctgccggtc
    10681 tccctccggc tcctgaagga ttgcgatgcc cagccccgaa cccaccgtct ccgccgcccc
    10741 gcgcggcgcc acgctcgcct tccccaccct ccgtacgccc gccggaccgg taccgcccga
    10801 cgcaccggcg tggaatccgc agcgcggcag cgcgatgccc tcgcatcggt accggcccgc
    10861 gcacgaccgc gtcgaagtac cgcgtatcga acgcgcctgg cccgccgccc ggatgacgcg
    10921 ggccccgctg tgggtgccgg tcgatctgcg cgacggcaac caggcgctgg ccgagccgat
    10981 ggac
//
