LOCUS       NZ_CP084681            10843 bp    DNA     linear   CON 30-APR-2024
DEFINITION  Streptomyces inhibens strain NRRL30439 chromosome, complete genome.
ACCESSION   NZ_CP084681
VERSION     NZ_CP084681.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces inhibens
  ORGANISM  Streptomyces inhibens
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 9696490)
  AUTHORS   Barry,C.P., Gillane,R., Talbo,G.H., Plan,M., Palfreyman,R.,
            Haber-Stuk,A.K., Power,J., Nielsen,L.K. and Marcellin,E.
  TITLE     Multi-omic characterisation of Streptomyces hygroscopicus NRRL
            30439: detailed assessment of its secondary metabolic potential
  JOURNAL   Mol Omics 18 (3), 226-236 (2022)
   PUBMED   34989730
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9696490)
  AUTHORS   Barry,C.P., Palfreyman,R. and Marcellin,E.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-OCT-2021) EMS, Australian Institute for Bioengineering
            and Nanotechnology, Cnr College Rd & Cooper Rd, Brisbane, Queensland
            4103, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. APRIL-2018
            Assembly Name                     :: AIBN_shyg
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 239.0x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022221585.1-RS_2024_04_30
            Annotation Date                   :: 04/30/2024 04:58:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,775
            CDSs (total)                      :: 8,682
            Genes (coding)                    :: 8,305
            CDSs (with protein)               :: 8,305
            Genes (RNA)                       :: 93
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 158 of 377
            Pseudo Genes (incomplete)         :: 310 of 377
            Pseudo Genes (internal stop)      :: 38 of 377
            Pseudo Genes (multiple problems)  :: 116 of 377
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 23:32:03
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 7550650
            Orig. end                         :: 7561493
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP084681.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Australian Institute for
            Bioengineering and Nanotechnology, Building 75,Cnr College Rd &
            Cooper Rd, Brisbane City QLD 4072.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10843
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="24"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10843
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10843
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5843](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5843)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    116..130
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 18.71"
                     /tool="antismash"
     gene            complement(750..2438)
                     /locus_tag="KI385_RS33925"
                     /old_locus_tag="KI385_33995"
     CDS             complement(750..2438)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (93-292). E-value:
                     8.5e-17. Score: 53.5. Matches aSDomain:
                     nrpspksdomains_KI385_RS33925_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016432060.1"
                     /locus_tag="KI385_RS33925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KI385_33995"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_238575876.1"
                     /sec_met_domain="PF00561 (E-value: 2.1e-24, bitscore: 85.5,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRALALYGTIGSLIVTALATAPAGGATRASSAPPAPVAHRIDFGR
                     CAPVEHLPSSVECGKLTVPLDYARPDGKKIGLTVSRIRATTHGERQGSLVFNPGGPGAS
                     SMEFPLYGALPKWSKLARAYDFVGYAPRGVGRSAPLSCQDPEEFVKAPTDSWMHPDADF
                     KRKKVAQARAYARGCARRAGADLPFYNSVNNARDLDMLRAALGDKKLTFMGASYGTYFG
                     AVYATLFPGHVRRMIFDSVVNPSPRKIWYHNNLDQSIAFERRWGDWLRWVARHDDHYHL
                     GKSRRAVQHAYDRATHKLRRDPLSGKIGPGQLQSAFLKTGYNDAFWPMRAEALSGYLHG
                     DPKPLIAQALPRSGGAKDAENGNAVYTAVECNDAIWPRSWKTWNRDNTDIARTAPFETW
                     DNAWMNLPCAFWPDRSESAEAGLDAAVRHATGRDAGQELRDATGHAAETALDDAFHGRH
                     GPLDVHTEPGALPPVLLMAAERDAATPYRGALELQRRFPGSSLVTENGAGTHGIAFGSN
                     ACVNAYAEAYLLRGKIPAHKVYCPRRAEPVPGQQVTRAQHARPGK"
     aSDomain        complement(1563..2159)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_KI385_RS33925_Abhydrolase_1.1"
                     /evalue="8.50E-17"
                     /label="KI385_RS33925_Abhydrolase_1.1"
                     /locus_tag="KI385_RS33925"
                     /protein_end="292"
                     /protein_start="93"
                     /score="53.5"
                     /tool="antismash"
                     /translation="LVFNPGGPGASSMEFPLYGALPKWSKLARAYDFVGYAPRGVGRSA
                     PLSCQDPEEFVKAPTDSWMHPDADFKRKKVAQARAYARGCARRAGADLPFYNSVNNARD
                     LDMLRAALGDKKLTFMGASYGTYFGAVYATLFPGHVRRMIFDSVVNPSPRKIWYHNNLD
                     QSIAFERRWGDWLRWVARHDDHYHLGKSRRAVQHAY"
     gene            complement(2523..3332)
                     /locus_tag="KI385_RS33930"
                     /old_locus_tag="KI385_34000"
     CDS             complement(2523..3332)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088889.1"
                     /locus_tag="KI385_RS33930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KI385_34000"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_238575877.1"
                     /transl_table=11
                     /translation="MWLLFLLVAWVAAVLSCARVCRAAVAAARTTGPAPRPAPRTTLDP
                     SAEDHPGPGLTLYEMAYLSGGPHRLADVVLVLMAQKRRLLLAHTGWATVVEPVAQDAVE
                     RATLTAIGPEGQRRIPAIRDALVADDAVRAVADRLVAAGLALPAAARPGIRGAVRLVQG
                     AALLVLLSAAASYWMAPSGEDNGLVLAWFALPLILTLGTWAVARFELHPYHDWATDAGE
                     RCLPDAQQSPGTPGSPAAPAPLTTLALHGPTALTDPSLRAALHSSGA"
     misc_feature    complement(2826..2828)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    3366..3381
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 18.07"
                     /tool="antismash"
     misc_feature    3373..3387
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.13"
                     /tool="antismash"
     gene            complement(3432..4472)
                     /locus_tag="KI385_RS33935"
                     /old_locus_tag="KI385_34005"
     CDS             complement(3432..4472)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014175676.1"
                     /locus_tag="KI385_RS33935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KI385_34005"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_238575878.1"
                     /transl_table=11
                     /translation="MFFEHVSSFAQWVYLGIGASSLVLIIGAILVRLRTPSVRKADAVT
                     HEVWEMAFLTGGPGRVVDAAIAGMHEDGRLTVGGPGVVTVRQATPHDAVEAAVLEAVAS
                     TPGGALTTLRATAMRSPAVQGIGDRLAARGLLRHPRLGRGWRRWASAQMAACAVLFFVG
                     VLISIIGNGWDDASDAPPVITIVPALIGGLIVGSLCRKGFTHRITKAGSFALRAAKTAN
                     PAGGVGGAWQAAGLVVALGGTALITDELLRQQFAEAQHVAAAAASSGGSASSGSSSGYD
                     SGGGSSGDSTFWCGSSGGGSGSSCGSGGSSCGAGASCGGGGSSCGGGSSCGGGSSCGGG
                     SGCGSS"
     misc_feature    complement(4466..4480)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 17.56"
                     /tool="antismash"
     gene            complement(4478..5017)
                     /locus_tag="KI385_RS33940"
                     /old_locus_tag="KI385_34010"
     CDS             complement(4478..5017)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="KI385_RS33940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="KI385_34010"
                     /product="hypothetical protein"
                     /protein_id="WP_238575879.1"
                     /transl_table=11
                     /translation="MPRWLEPVADTVPDTRPEANAGTHAGTHADAHPDAHPDTGTDAAR
                     QRLALAQTALLSALVAGTPAPEGFDRARLRVQSRALTAKRAAVVAKVAPELPEILGEGH
                     RAAFVGYARHRPMSGGYRQDALDFAAHLLAQGRPEDPAARQQLTRWWQDRAGPKPPSAR
                     PMARLTRAVRRALHQR"
     gene            complement(5001..5843)
                     /locus_tag="KI385_RS33945"
                     /old_locus_tag="KI385_34015"
     CDS             complement(5001..5843)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF003818.1"
                     /locus_tag="KI385_RS33945"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KI385_34015"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_238575880.1"
                     /sec_met_domain="DUF692 (E-value: 8.3e-96, bitscore: 319.3,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MERLGTGIGWRPEIAAEIAELPGVDWVEVVAENICPGHLPYALQE
                     LRERGTTVIPHGVSLGLGGADRPDTGRLTALAERAEALGAPLVTEHIAFVRAGGPLTAS
                     PVMEAGHLLPVPRTRDALRVLCENVRIAQDALPVPLALENIAALINWPGEELTEGQFLS
                     ELVEQTGVRLLIDVANLHTNHINRGEDPAVALDELPVEAIAYVHVAGGVERDGVWHDSH
                     AHPVTRPVLNVLAELCARITPPGVLLERDDDFPEGGELAGELDAIRTVLKEARHAQVA"
     gene            complement(6220..6696)
                     /locus_tag="KI385_RS33950"
                     /old_locus_tag="KI385_34020"
     CDS             complement(6220..6696)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1135: MarR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014057926.1"
                     /locus_tag="KI385_RS33950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KI385_34020"
                     /product="MarR family transcriptional regulator"
                     /protein_id="WP_238575881.1"
                     /transl_table=11
                     /translation="MENNVGREIADALGVLLKRSTRIELHRTLTAGMGEAVDELTYPIL
                     SGLDRTGPRSAADLAPDVGLDRSNVTRRASRLEAAGLVRREPDPSDRRATLLALTDEGE
                     RTVETLRRRLSDHITASLSSWPADEAAAFASHLRRFVADGPFTRPRRLRGDSLP"
     gene            6802..7194
                     /locus_tag="KI385_RS33955"
                     /old_locus_tag="KI385_34025"
     CDS             6802..7194
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016643719.1"
                     /locus_tag="KI385_RS33955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KI385_34025"
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="WP_238575882.1"
                     /transl_table=11
                     /translation="MNRTHIAAALTDLIFNPGLDLHEAADRHFAPDYRQRTNGQWADRE
                     GFLEHIAHLRTVVAEGSVEVHDELSDGSHYADRHTVHVNKKDGSTVRTEVYLFADFAPD
                     GRFSRIEEVTLMLEGDEADRNLGSAR"
     gene            complement(7795..8574)
                     /locus_tag="KI385_RS33960"
                     /old_locus_tag="KI385_34030"
     CDS             complement(7795..8574)
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_KR (18-175). E-value: 1.1e-11.
                     Score: 36.9. Matches aSDomain:
                     nrpspksdomains_KI385_RS33960_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006604763.1"
                     /locus_tag="KI385_RS33960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KI385_34030"
                     /product="SDR family oxidoreductase"
                     /protein_id="WP_238575883.1"
                     /sec_met_domain="adh_short (E-value: 6.3e-32, bitscore:
                     110.0, seeds: 230, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTATATASERRPTAERPRTAVVTGASSGIGAATARRLASEGYHVA
                     LAARRTDRIKELADEIGGTAYTLDVTDETAVAEFAGRLDRVDVLVNNAGGALGSEPVAD
                     ADPAEWRRMYEVNVLGVLHVTRALLPKLTASGDGTVLIVSSTAGHGAYEGGGGYAAAKF
                     AAHALAVTLRLELCGAPVRVIEIAPGMVRTEEFALNRFRGDRTAADRVYAGVAMPLTAD
                     DVADTIAWSVTRPSHVNIDLLVVRPRAQAAQHKVHRT"
     aSDomain        complement(8050..8520)
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_KI385_RS33960_PKS_KR.1"
                     /evalue="1.10E-11"
                     /label="KI385_RS33960_PKS_KR.1"
                     /locus_tag="KI385_RS33960"
                     /protein_end="175"
                     /protein_start="18"
                     /score="36.9"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="TAVVTGASSGIGAATARRLASEGYHVALAARRTDRIKELADEIGG
                     TAYTLDVTDETAVAEFAGRLDRVDVLVNNAGGALGSEPVADADPAEWRRMYEVNVLGVL
                     HVTRALLPKLTASGDGTVLIVSSTAGHGAYEGGGGYAAAKFAAHALAVTLRLE"
     CDS_motif       complement(8428..8514)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_KI385_RS33960_0001"
                     /evalue="1.30E-04"
                     /label="PKSI-KR_m1"
                     /locus_tag="KI385_RS33960"
                     /protein_end="49"
                     /protein_start="20"
                     /score="14.7"
                     /tool="antismash"
                     /translation="VVTGASSGIGAATARRLASEGYHVALAAR"
     gene            complement(8571..9164)
                     /locus_tag="KI385_RS33965"
                     /old_locus_tag="KI385_34035"
     CDS             complement(8571..9164)
                     /GO_function="GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018539783.1"
                     /locus_tag="KI385_RS33965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KI385_34035"
                     /product="YbaK/EbsC family protein"
                     /protein_id="WP_238575884.1"
                     /transl_table=11
                     /translation="MTAHTVSPDAPARADAAREAGSTPGTTSDRLLALLQDGRARHQLI
                     EHAPEGRTDVVSALRGNALDQAAKCIIMRVKVTKKSSRYALAVVPGDRRVDLGRVKELY
                     DARHVSFADPATAERLSGCVSGSIVPFSFDPTLELIVDPGLLEHREIFFNAAELHLSLA
                     LDTEDYRRLARPRVAPIAEPIAEPATEPATRTAS"
     gene            complement(9161..9970)
                     /locus_tag="KI385_RS33970"
                     /old_locus_tag="KI385_34040"
     CDS             complement(9161..9970)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="KI385_RS33970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="KI385_34040"
                     /product="hypothetical protein"
                     /protein_id="WP_238575885.1"
                     /transl_table=11
                     /translation="MSTTDAWTRVVIDAAAERPDADLLRRLAHGETAVVVLRNLLPAEA
                     FADNRTRIGKLFANASTTSYANGALTTIGPYLAKYLQDLDGYFQAADEAKGMLDAADFD
                     LADQVRARLRDAFGLDAVSVAQEPDGRDYAASVVRIHADGVRNPLHNDNIMRDAQGTGI
                     ALTGLAYQLSCVVCLQECDGGGELRMYRKPWEPADETYKIQDGLGYDEAVVDGYPCHEF
                     KPQTGDVYLINPMQYHSIERVTGSDRLTMGFFMGFGTDTLNSAVVWG"
     gene            complement(9957..10727)
                     /locus_tag="KI385_RS33975"
                     /old_locus_tag="KI385_34045"
     CDS             complement(9957..10727)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="KI385_RS33975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="KI385_34045"
                     /product="hypothetical protein"
                     /protein_id="WP_238575886.1"
                     /transl_table=11
                     /translation="MCRLIVASGDFRAADILAAAEAMSCGRTADHDGPTEVHPNGWGAV
                     WRQADAPYGLGRHRDVRPLTESFRESPVPDLTTDFLAVHARHATLAKNHGPRFTHPLER
                     TGGAVPWLFMHNGFLPTVHRLLGMESSEFDSREYFDYVVPAGARRLDEAETLDRLNSIP
                     RGGSSGNAIAVNPYSAYVIHWTSPDTVSPRYFGMFRLQTDRSLVIASEVVPDLAPADHW
                     EPLKPDTVTEIPWTTGLTPRNRYDLEGAPTHVHD"
ORIGIN
        1 acgaggtcat ccgctacacc ctctggtcgg tgttcaagct gcgcgatgtc ctcccggagg
       61 accgcagcgg ctacgcagac gaggtcgagg agctgttcga gcagctcgcc gccaaggacg
      121 tggtggtccg cggcacgtac gacgtgtccg gcctgcgcgc cgacgccgac ctgatgatct
      181 ggtggcactc ggagagctcg gacgcgctcc aggaggcgta caacctcttc cgccgcaccc
      241 gtctcggccg ggcgcttacg ccggtgtggt cgaacatggc gctgcaccgc cccgccgagt
      301 tcaacaagtc gcacatcccg gccttcctgg ccgacgaggt ggcccgcgac tacgtcagcg
      361 tctacccctt cgtgcgctcc tacgagtggt acctcctccc cgacgaggac cgccgccgga
      421 tgctcgccga ccacggcaag atggcccgcg gcttcccgga cgtgcgcgcc aacaccgtgc
      481 cctcgttctc gctcggcgat tacgagtggg tgctggcgtt cgaggccgat gagctctacc
      541 gcatcgtcga cctgatgcgt catctgcgcg gctccgaggc gcggatgcac gtccgcgagg
      601 aggtcccgtt ctacaccggc cggcgcaagc cggttgccga gctggtcgcc ggcctggcct
      661 gatccggggc cggcacgcag cgagcgtgca cacagcgata cgggccgccc cccttggggg
      721 acggcccgta tcgcatccgg cgggtgccgc tacttccccg gccgcgcgtg ctgcgcccgg
      781 gtcacctgct ggcccggcac cggctcggcg cgccgcgggc agtacacctt gtgcgccggg
      841 atcttcccgc gcagcagata cgcctccgcg tacgcgttca cacaggcgtt gctgccgaag
      901 gcgatgccgt gggtgcccgc accgttctcg gtgaccagtg aggagccggg gaaccgccgc
      961 tgcagctcca acgctccccg gtacggcgtc gccgcgtccc gctcggccgc catcagcagc
     1021 accggcggca gtgcgcccgg ctcggtgtgg acgtcgagcg gaccgtgccg cccgtggaac
     1081 gcatcgtcca gcgcggtctc ggccgcatgc ccggtggcgt ccctcagctc ctgtcccgca
     1141 tcgcggccgg tcgcatggcg gaccgcggcg tcgagaccgg cctcggccga ctccgaccgg
     1201 tcgggccaga aggcgcacgg cagattcatc caggcgttgt cccaggtctc gaacggcgcg
     1261 gttcgcgcga tatcggtgtt gtcgcggttc caggtcttcc aggagcgcgg ccagatcgcg
     1321 tcgttgcact ccacggcggt gtagacggcg ttgccgttct ccgcgtcctt ggcgccgccg
     1381 gaccggggca gggcctgcgc gatcagtggc ttggggtcgc cgtgcagata ccccgacagc
     1441 gcctcggcgc gcatcggcca gaacgcgtcg ttgtagccgg tcttgaggaa ggccgactgc
     1501 agctgaccgg ggccgatctt gccgctcagc ggatcgcggc gcagcttgtg ggtggccctg
     1561 tcgtaggcgt gctgcaccgc cctcctggac ttgccgaggt ggtagtggtc atcgtgccgg
     1621 gcgacccagc gcagccagtc gccccagcgc cgctcgaagg cgatgctctg gtcgaggttg
     1681 ttgtggtacc agatcttccg gggtgaggga ttgacgacgc tgtcgaagat catccgccgg
     1741 acatggccgg ggaagagcgt cgcatagacc gcaccgaagt acgtcccgta cgaggcgccc
     1801 atgaacgtca gcttcttgtc gccgagggcc gcccgcagca tgtccaggtc gcgggcgttg
     1861 ttgacggagt tgtagaacgg cagatcggcg ccggcccggc gggcacagcc gcgggcatac
     1921 gccctcgcct gcgcgacctt cttgcgcttg aagtccgcat ccgggtgcat ccagctgtcg
     1981 gtgggcgcct tgacgaactc ctccgggtcc tggcacgaca gcggcgccga gcggccgacc
     2041 ccgcgcggcg catagccgac gaagtcgtag gcgcgggcca gtttgctcca cttggggagc
     2101 gcgccgtaga gcgggaactc catgctggac gcgccggggc cgccggggtt gaagaccagg
     2161 gagccctgcc gttcgccgtg cgtggtggcg cggatccggc tcacggtgag gccgatcttc
     2221 ttcccgtcgg ggcgcgcgta gtcgagggga acggtgagct tgccgcactc gacggatgac
     2281 ggcagatgct ccaccggtgc gcaccggccg aagtcgatgc ggtgcgcaac gggcgcgggc
     2341 ggcgcggagg aggccctcgt ggcgccgcct gccggagcag tggcgagggc ggtcacgatc
     2401 agcgacccga tggtgccgta caacgcaagt gctctcactg gccgtccctt cgtgtgtgca
     2461 tacaaaaggg atagttgagg cggtggttgt cgatgtaaag cacctgggtc cggtgtcgga
     2521 cgctatgccc cggaagagtg gagcgcggcg cgcagggagg ggtcggtgag ggcggtgggg
     2581 ccgtggagag caagggtggt gaggggggca ggggcggcgg gggagcccgg agtgccgggg
     2641 gattgctgag catcgggcag gcaccgttcg cccgcgtcgg ttgcccagtc gtggtacgga
     2701 tggagctcga agcgggccac cgcccacgtg ccgagggtga ggatcagcgg gagcgcgaac
     2761 caggccagga ccagcccgtt gtcctcgccg gacggcgcca tccagtagct cgccgcggcg
     2821 gacagtaaca ccaggagggc ggcgccctgc accaggcgga ccgcgccgcg gatgcccgga
     2881 cgggccgcgg cgggcagcgc gagcccggcg gccaccagtc ggtcggccac cgcccgcacc
     2941 gcgtcgtcgg cgacgagggc gtcccgtatc gcgggtatcc ggcgctggcc ttcgggtcct
     3001 atggcggtga gggtggcgcg ctcgacggcg tcctgggcga ccggctcgac gacggtggcc
     3061 cagccggtgt gggcgagcag cagccgccgt ttctgggcca tcagcaccag gaccacatcg
     3121 gcgagccggt gcgggccacc ggagagatag gccatctcgt agagggtcag tccgggcccg
     3181 gggtggtctt cggcggacgg atccagggtg gtgcggggcg ccggccgcgg ggccggtccg
     3241 gtggtgcggg cggcggccac cgcggcacgg cagacccggg cgcaggagag gaccgccgcc
     3301 acccaggcga cgaggaggaa gagaagccac atagcggcag gtttacggga cagggcatga
     3361 cagccgccac gaatttttct cacatccggg cggacgcaag cgatttacgg gccggcgcgg
     3421 gggacgcggc gtcagctgct gccgcagccg gacccgccgc cgcagctcga tccgccgccg
     3481 caggacgagc cgcccccgca gctcgaaccg cccccgccgc acgacgcccc ggcgccgcac
     3541 gaagaaccac cgctgccgca gctggacccc gagccgccgc cggaggagcc gcaccagaag
     3601 gtgctgtcac ctgacgagcc tccccccgag tcgtaaccgg acgaactgcc ggacgaggcg
     3661 gatccgccgg acgaagcggc ggctgccgcg acgtgttgcg cctccgcgaa ctgctgccgc
     3721 agcagctcgt ccgtgatcag cgccgtaccg ccgagcgcca ccacgagccc ggcggcctgc
     3781 caggccccgc ccacgccccc tgcggggttg gccgtcttcg cggcgcgcag ggcgaaggag
     3841 cccgccttgg tgatccgatg ggtgaacccc ttccggcaca gcgagcccac gatcaggccc
     3901 ccgatcaacg cgggcacgat cgtgatgacc ggcggcgcat cggacgcgtc gtcccagccg
     3961 ttgccgatga tggagatcag caccccgacg aagaagagca ccgcacacgc cgccatctgg
     4021 gcgctcgccc accggcgcca gccccggccg agccgcggat ggcgcagcag cccgcgggcg
     4081 gccagccggt cgccgatgcc ctggaccgcc gggctgcgca tcgcggtggc gcgcagcgtg
     4141 gtcagcgccc caccgggcgt cgaggcgacc gcttccagta cggccgcctc gaccgcgtcg
     4201 tgcggggtgg cctgacgtac cgtcaccacg cccgggccgc cgaccgtcag ccggccgtcc
     4261 tcatgcatac cggcgatcgc ggcgtccacc acccgcccgg ggccgccggt gaggaacgcc
     4321 atctcccaca cctcgtgggt cacggcatcc gccttgcgga cggacggggt ccgcaagcgc
     4381 accaggatcg cgccgatgat gagcaccagc gacgaggcgc cgatccccag gtacacccac
     4441 tgcgcgaagg aggacacgtg ctcgaagaac atggtggtca cctctggtgc agggcgcgcc
     4501 gcacggcgcg cgtcagtcgg gccatcggcc gggcggatgg cggcttcggg cccgcgcgat
     4561 cttgccacca gcgggtcaac tgctgccggg cggccgggtc ctcgggacgg ccctgggcca
     4621 gcagatgcgc cgcgaagtcc agcgcgtcct ggcggtagcc gccgctcatg ggccggtgcc
     4681 gcgcatagcc gacgaacgcg gcccggtggc cctcgccgag aatctcgggc agctcgggcg
     4741 cgaccttggc gacgaccgcg gctcgcttgg cggtcagcgc ccggctctgt acgcgcagtc
     4801 gtgcgcggtc gaagccttcc ggggccggcg tcccggcgac cagcgcggac agcagcgcgg
     4861 tctgcgccag cgccagacgc tgccgcgccg cgtcggtgcc cgtgtccggg tgcgcgtccg
     4921 ggtgcgcgtc cgcgtgggtg ccggcgtggg tgccggcgtt cgcttccggg cgtgtgtccg
     4981 gcacggtgtc cgcaaccggc tcaagccacc tgggcatggc gggcctcctt caacacggtg
     5041 cggatcgcgt cgagttcgcc ggccagctcc ccgccctcgg ggaagtcgtc atcgcgctcc
     5101 agcagcacgc cgggcggggt gatccgggca cacagctcgg cgagcacgtt cagcaccggc
     5161 cgggtcaccg ggtgggcatg gctgtcgtgc cacaccccgt cccgctccac accgcccgcg
     5221 acatggacgt acgcgatggc ctcgaccggc agctcgtcga gggcgacggc cgggtcctcg
     5281 ccccggttga tgtggttcgt atggaggttg gcgacatcga tgagcagccg caccccggtt
     5341 tgctcgacga gctccgacag gaactgcccc tcggtcagct cctcgcccgg ccagttgatg
     5401 agtgcggcga tgttctccag ggccagcggc accggcagcg cgtcctgggc gatccgtacg
     5461 ttctcgcaga gcacccggag tgcatcacgg gtacgcggaa ccggcagcag atgtccggcc
     5521 tccatgaccg gcgaggcggt gagcggcccg ccggcccgga cgaaggcgat gtgttcggtg
     5581 accagcggcg cgcccagcgc ctcggcacgc tcggcgagcg cggtcagccg gccggtgtcc
     5641 ggccggtcgg cgccgccgag gccgagcgag accccgtgcg gaatgacggt ggtgccgcgc
     5701 tcgcgcagct cctgcagggc gtacggcaga tggccggggc agatgttctc cgccacgacc
     5761 tcgacccagt ccaccccggg cagctccgcg atctccgcgg cgatctccgg ccgccagccg
     5821 attccggtac caagacgctc catggtcccc cctctcgctc cgtggtcggg gtactcatgg
     5881 ccgcgccacg gggggccgaa ccctgcaccg gccgagttca gaggttgatt tgagcttcgt
     5941 ccagcgccct gcgcagcgcg cggtggtcgg cgacgaccgt gcacgagccc ggtgcgatct
     6001 cgttcggggc caggaccttg caccgcgctc tcacctgctc attccctcct cctctcattc
     6061 cctgtccgtt cctgacagct gaggactcca cgggttgtga cgctggggcc tcggacaggg
     6121 acgcgttctg aacccgagac ccggtggtcg gacagccggg cgcttcggcc ggttctggcc
     6181 gcgcttgcgt gacgagtact ccaggaggac ggcctgcgtt caggggaggg agtcaccgcg
     6241 caaccggcgt ggccgggtga acggtccgtc ggcgacgaag cgccggaggt gactcgcgaa
     6301 ggctgcggct tcgtcggccg gccatgagga caggctggcc gtgatgtggt cggacaggcg
     6361 ccggcgcagg gtttccacgg tgcgttctcc ctcgtcggtg agggcgagca gagtggcgcg
     6421 ccggtcggag gggtcgggtt cgcgtcgtac gagtccggct gcctccagtc tggaggcgcg
     6481 gcgggtgaca ttggaccggt cgagccctac gtcgggagcc aggtcggccg cactgcgggg
     6541 cccggttctg tccagcccgc tgagaatcgg gtacgtcagc tcgtccaccg cttcccccat
     6601 gccggcggtc agggtccggt gcagctcaat gcgggtgctg cgtttgagca gcacaccgag
     6661 ggcatcggcg atctctcgcc ccacgttgtt ctccaccttc ccagaatagc gtgcgtctcg
     6721 cacgcatatg ggttacagtg acagggatgc gtgcgcagcg cacgcattgt cgaaacgtga
     6781 aacgcgaaaa ggaaacccgc catgaatcgc acacacatcg ccgcggcact caccgacctg
     6841 atcttcaacc ccggacttga cctccatgag gccgccgacc ggcacttcgc tcctgactat
     6901 cgtcagcgca ccaacggaca gtgggccgac cgcgaaggct tccttgagca catcgcccat
     6961 ctgcgcacgg ttgtggccga gggctccgtc gaggtccacg acgagctgtc cgacggcagc
     7021 cactacgccg accgccacac ggtccacgtc aacaagaagg acggatccac cgtgcgcacc
     7081 gaggtctacc tgttcgccga cttcgctccc gacggccgct tcagccgcat cgaggaagta
     7141 accctcatgc tcgaaggtga cgaggccgac cggaacctgg gcagcgcccg ctgaggcacg
     7201 acaccgagca cccgccggcg cggctggcca catacaaacc ttggccaaca gcggggggag
     7261 gagggctgac cgcccgccct gggggggcga tcttggtcgc ccccggctca gtgcacctat
     7321 gcgtgatctg tgggcggccg ccgctcgggc ggcaggcgga tcgagacctc ggtcaggcag
     7381 cccccggaaa gatccgtgca ggccgacctc cgcgaagtag gcggtggcgg agccgaggtc
     7441 gcggcgggta tggcccggct ccaggcgtcc gctgtaccag tgttcgtgtg caggggagag
     7501 cggcacgtca gcccggccgg accggtgtgg cgccggcacc tctgccggac cggcagggtc
     7561 gggtagacct ggccgctgtg gcgcgggccc gaagtctgct gaggcagcgt ttcgccgaga
     7621 atatcggtgc cgatgagctg gcccggccgg ccggatgccc cgctgagcgc ggcgaacatg
     7681 ttttggtggg ctcgtcgaca gcaccggccg gttgtagctg tcgtccaccg gccggtggac
     7741 ccgtggacgg cggggcatcc ctgtcaccca cggggtccgc gcggcgcggt gccgctacgt
     7801 gcggtgcacc ttgtgctggg cggcctgcgc ccgtgggcgt accaccagca ggtcgatgtt
     7861 cacatggctc gggcgggtga ccgaccaggc gatcgtgtcg gcgacgtcat cggccgtcag
     7921 cggcatcgcc acgcccgcgt acacccggtc ggcggccgtg cggtcgccgc ggaagcggtt
     7981 cagcgcgaac tcctcggtgc gcaccatgcc gggggcgatc tcgatcaccc gtaccggtgc
     8041 gccgcacagt tcgagccgca gggtcacggc gagcgcgtgc gcggcgaact tggccgccgc
     8101 gtaccctccg ccgccttcgt acgccccatg cccggcggtg gaggacacga tcaggaccgt
     8161 gccgtcgcca ctggcggtca gcttcggcag cagcgcccgt gtgacatgca ggacgccgag
     8221 gacgttcacc tcgtacatcc gccgccactc cgccgggtcc gcgtccgcga ccggctcgct
     8281 ccccagcgcg ccacccgcgt tgttcaccag gacgtccacc cggtccagcc ggccggcgaa
     8341 ctccgccacc gctgtctcgt ccgtgacgtc cagcgtgtac gcggtgccgc cgatctcgtc
     8401 ggcgagttcc ttgatgcggt cggtgcggcg ggcggcgagc gcgacgtggt atccctcgct
     8461 cgccagcctg cgggcggtgg ccgcgccgat gccgctgctc gcgccggtga ccacggcggt
     8521 gcgcggccgt tccgccgtcg gccgtcgttc cgaagccgtc gctgtcgcgg tcatgatgcc
     8581 gtcctcgtcg cgggctcggt ggcgggctcg gcgatgggct cggcgatggg cgccacccgc
     8641 ggccgggcca gtcgccggta gtcctccgtg tccagggcca gggacagatg cagctcggcc
     8701 gcgttgaaga agatctcccg gtgctccagc aggccggggt cgacgatcag ctccagcgtg
     8761 gggtcgaagg agaacggcac gatggagccg ctgacgcagc cggacaggcg ttccgcggtg
     8821 gccgggtcgg cgaaggacac atgccgtgcg tcgtacagct ccttgacccg gccgaggtcc
     8881 acccgccggt caccggggac gacggcgagg gcgtaacgcg aggacttctt ggtgaccttg
     8941 acgcgcatga tgatgcactt ggccgcctgg tccagggcgt tcccgcgcag cgcgctgacc
     9001 acgtccgtac gcccctccgg ggcatgttcg atcagctggt gacgggcccg gccgtcctgc
     9061 agcagggcca gcagccggtc cgatgtcgtg ccgggcgtgc tgcccgcttc ccgtgccgcg
     9121 tccgcccgcg cgggggcgtc gggagagacc gtgtgtgccg tcatccccac accaccgcgg
     9181 aattgagcgt gtcggtgccg aagcccatga agaagcccat ggtgagccgg tcggatccgg
     9241 tgacccgctc gatggagtgg tactgcatcg ggttgatcag gtacacgtct ccggtctgcg
     9301 gcttgaactc gtggcagggg tacccgtcga ccacggcctc gtcgtacccg agaccgtcct
     9361 ggatcttgta cgtctcgtcc gcgggctccc agggcttgcg gtacatacgc agctcgccgc
     9421 ccccgtcgca ctcctgcagg cagaccacgc agctcagctg gtacgcgagg ccggtgaggg
     9481 cgatgccggt gccttgcgcg tcccgcatga tgttgtcgtt gtgcagcggg ttgcggacgc
     9541 cgtcggcgtg gatgcggacg acggacgccg cgtagtcgcg gccgtccggc tcctgcgcga
     9601 ccgacaccgc gtcgaggccg aaggcgtccc tgagccgggc gcggacctgg tcggcgaggt
     9661 cgaagtcggc ggcgtccagc atgcccttgg cctcgtcggc ggcctggaag taaccgtcca
     9721 ggtcctggag gtacttggcc aggtaggggc cgatggtggt gagggcgccg ttggcgtagc
     9781 tcgtcgtgga ggcgttggcg aagagcttgc cgatgcgggt ccggttgtcg gcgaacgcct
     9841 cggcgggcag caggttgcgc agcacgacca cggcggtctc gccgtgggcc agccggcgga
     9901 gcagatcggc gtccgggcgc tcggcggcgg cgtcgatgac aacgcgggtc caggcatcag
     9961 tcgtggacat gggtcggtgc tccttcgagg tcgtaacggt tgcggggggt gaggcccgtg
    10021 gtccacggga tctccgtgac ggtgtccggc ttcagcggct cccagtggtc cgcgggggcg
    10081 aggtcgggga ccacctccga ggcgatcacc aaagagcggt cggtctgcag ccggaacatg
    10141 ccgaagtacc gcggcgagac ggtgtccggt gaggtccagt ggatgacgta ggcggagtag
    10201 gggttcaccg cgatggcgtt acccgaactc ccacccctgg gaatggagtt gagccggtcg
    10261 agggtttccg cctcgtccag ccggcgggcg ccggcgggca cgacgtagtc gaagtactcg
    10321 cgggagtcga actccgatga ctccatgccg agcaggcggt ggaccgtcgg caggaagccg
    10381 ttgtgcatga acagccaggg gacggcgccg ccggtgcgtt ccagggggtg ggtgaagcgc
    10441 gggccgtggt tcttggccag tgtggcgtgg cgtgcgtgca cggccaggaa gtcggtcgtg
    10501 agatccggta cgggcgactc gcggaagctc tccgtgaggg ggcgcacgtc ccggtggcgg
    10561 cccaggccgt acggtgcgtc cgcctggcgc cataccgcgc cccagccgtt ggggtgcacc
    10621 tcggtgggac cgtcgtggtc ggcggtgcgg ccacagctca tcgcctcggc cgcggccagg
    10681 atatccgcgg cacggaagtc ccctgaggcc acgatgagtc ggcacatgaa aggtcagcct
    10741 cgcaatcggt gggcaacgag tgaatgcagc agtgcgggga tccggtcgcc ggggccgccg
    10801 ctgacgacga cccggccgac cgcgccggtg tgccgggcga ggt
//
