LOCUS       NZ_CP084681            10417 bp    DNA     linear   CON 30-APR-2024
DEFINITION  Streptomyces inhibens strain NRRL30439 chromosome, complete genome.
ACCESSION   NZ_CP084681
VERSION     NZ_CP084681.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces inhibens
  ORGANISM  Streptomyces inhibens
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 9696490)
  AUTHORS   Barry,C.P., Gillane,R., Talbo,G.H., Plan,M., Palfreyman,R.,
            Haber-Stuk,A.K., Power,J., Nielsen,L.K. and Marcellin,E.
  TITLE     Multi-omic characterisation of Streptomyces hygroscopicus NRRL
            30439: detailed assessment of its secondary metabolic potential
  JOURNAL   Mol Omics 18 (3), 226-236 (2022)
   PUBMED   34989730
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9696490)
  AUTHORS   Barry,C.P., Palfreyman,R. and Marcellin,E.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-OCT-2021) EMS, Australian Institute for Bioengineering
            and Nanotechnology, Cnr College Rd & Cooper Rd, Brisbane, Queensland
            4103, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. APRIL-2018
            Assembly Name                     :: AIBN_shyg
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 239.0x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022221585.1-RS_2024_04_30
            Annotation Date                   :: 04/30/2024 04:58:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,775
            CDSs (total)                      :: 8,682
            Genes (coding)                    :: 8,305
            CDSs (with protein)               :: 8,305
            Genes (RNA)                       :: 93
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 158 of 377
            Pseudo Genes (incomplete)         :: 310 of 377
            Pseudo Genes (internal stop)      :: 38 of 377
            Pseudo Genes (multiple problems)  :: 116 of 377
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 23:32:03
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2555184
            Orig. end                         :: 2565601
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP084681.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Australian Institute for
            Bioengineering and Nanotechnology, Building 75,Cnr College Rd &
            Cooper Rd, Brisbane City QLD 4072.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10417
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="16"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10417
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10417
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5417](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5417
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            complement(1288..2379)
                     /locus_tag="KI385_RS11905"
                     /old_locus_tag="KI385_11950"
     CDS             complement(1288..2379)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006606343.1"
                     /locus_tag="KI385_RS11905"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KI385_11950"
                     /product="amidohydrolase family protein"
                     /protein_id="WP_238577662.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 1.9e-15, bitscore:
                     55.9, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSEGAVLHLKGRVLVGPDDVRDELWIVGGRVTYDRPAMARDATTV
                     EGWALPGLVDAHCHVGLDAHGPVDAPTSEKQALTDRDAGTLLIRDAGSPSDTRWIDDRE
                     DLPRIIRAGRHIARTKRYIRNYAYEIEPDDLVAYVAREARRGDGWVKLVGDWIDRSTGD
                     LSACWPPGAVEAAIAEAHRLGARVTAHCFAEDSLAPLVEAGIDCIEHATGLTEETIPLF
                     AERGVAIVPTLVNIATFPQLAAGGEAKYPRWADHMRRLHERRYDTVRAAYDAGVPIFAG
                     TDAGGSLAHGLVAEEVVELTKAGLPAVAALSATTWGARDWLGRPGLTEGASADLVVYES
                     DPREDVRVLAAPRRVVLRGQVVG"
     misc_feature    complement(2508..2519)
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 19.75"
                     /tool="antismash"
     misc_feature    2619..2632
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 17.04"
                     /tool="antismash"
     misc_feature    complement(2810..2825)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.62"
                     /tool="antismash"
     misc_feature    complement(2820..2835)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 16.52"
                     /tool="antismash"
     gene            2991..3557
                     /gene="ectA"
                     /locus_tag="KI385_RS11910"
                     /old_locus_tag="KI385_11955"
     CDS             2991..3557
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014156969.1"
                     /locus_tag="KI385_RS11910"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KI385_11955"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_238572163.1"
                     /transl_table=11
                     /translation="MTAAQADHARARSDIGEFPEGFKLDTPRVEDGAAIWRIARDSKAL
                     DLNSSYSYLLWCRDFAATSVVARDTEGEPAAFVTGYIRPERPETLVVWQVAVGEAHRGR
                     GLAAALLDGLTTRAMDELGIRFVETTVTPDNTASNRMFASFAERHSVPVRREVLFDAGL
                     FPEQGHEPEVLHLIGPFTPPAPPGR"
     gene            3686..4954
                     /gene="ectB"
                     /locus_tag="KI385_RS11915"
                     /old_locus_tag="KI385_11960"
     CDS             3686..4954
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (29-364). E-value: 1.2e-75.
                     Score: 246.6. Matches aSDomain:
                     nrpspksdomains_KI385_RS11915_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448159.1"
                     /locus_tag="KI385_RS11915"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KI385_11960"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_238572164.1"
                     /sec_met_domain="Aminotran_3 (E-value: 1.9e-77, bitscore:
                     259.6, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITQPDLSVFETVESEVRSYCRGWPTIFDRAQGSRITDEDGHTY
                     LDFFSGAGSLNYGHNNPVLKRALIDYIERDGITHGLDMSTTAKRAFLESFQNIVLRPRD
                     LPYKVMFPGPTGANSVEAALKLARKVKGRESIVSFTNAFHGMSLGALAVTGNSMKRAGA
                     GIPLVHGTPMPFDNYLDGAYPDFLWFERLLGDQGSGLNTPAAVIVETVQGEGGINVARP
                     EWLRALADLCERRDMLLIVDDIQMGCGRTGAFFSFEEAGIVPDIVTVSKSISGYGLPLA
                     LTLFKPELDIWEPGEHNGTFRGNNPAFVTAAAALDTYWADGQMEKQTLARGEIVEAHLK
                     AIVEEHPDAIAEYRGRGLVWGLECIDKSLAGKIATRAFELGLLIETSGPESEVVKLLPA
                     LTTTPEELDEGLRTLARAVRDCA"
     aSDomain        3773..4777
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_KI385_RS11915_Aminotran_3.1"
                     /evalue="1.20E-75"
                     /label="KI385_RS11915_Aminotran_3.1"
                     /locus_tag="KI385_RS11915"
                     /protein_end="364"
                     /protein_start="29"
                     /score="246.6"
                     /tool="antismash"
                     /translation="DRAQGSRITDEDGHTYLDFFSGAGSLNYGHNNPVLKRALIDYIER
                     DGITHGLDMSTTAKRAFLESFQNIVLRPRDLPYKVMFPGPTGANSVEAALKLARKVKGR
                     ESIVSFTNAFHGMSLGALAVTGNSMKRAGAGIPLVHGTPMPFDNYLDGAYPDFLWFERL
                     LGDQGSGLNTPAAVIVETVQGEGGINVARPEWLRALADLCERRDMLLIVDDIQMGCGRT
                     GAFFSFEEAGIVPDIVTVSKSISGYGLPLALTLFKPELDIWEPGEHNGTFRGNNPAFVT
                     AAAALDTYWADGQMEKQTLARGEIVEAHLKAIVEEHPDAIAEYRGRGLVWGLEC"
     gene            5001..5417
                     /locus_tag="KI385_RS11920"
                     /old_locus_tag="KI385_11965"
     CDS             5001..5417
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006606340.1"
                     /locus_tag="KI385_RS11920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KI385_11965"
                     /product="ectoine synthase"
                     /protein_id="WP_128511240.1"
                     /sec_met_domain="ectoine_synt (E-value: 1.7e-53, bitscore:
                     178.7, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFKDIEGTDRHVKAKSGTWESKRIVLAKERVGFSLHETILY
                     AGTETSMWYANHIEAVLCVEGEAELTNDETGEKYTITPGTMYLLDGHEKHTMRIKEDFR
                     CVCVFNPPVTGREDHDENGVYPLLTEPEAEPEAV"
     gene            5516..6415
                     /gene="thpD"
                     /locus_tag="KI385_RS11925"
                     /old_locus_tag="KI385_11970"
     CDS             5516..6415
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014156966.1"
                     /locus_tag="KI385_RS11925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KI385_11970"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_238572165.1"
                     /sec_met_domain="PhyH (E-value: 2.6e-37, bitscore: 128.1,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTAPERIADLYPTRGTAEVITPRKDPVVWSQPGAAGPFAPSELS
                     DFERDGFFAIGELLTAEEVAVYRAELDRLVLDPAMRADPRSIVESKSQSVRSVFEVHKI
                     SEVFARLVSDPRVVGRARQILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLP
                     NMRTVSVSIALTENYDTNGGLMIMPGSHQHFVGCEGATPKDNYKRSLQMQDAGIPSDEV
                     LTKMADRHGIRLFTGKAGSATWFDCNAMHGSGDNITPYPRSNVFIVFNSVDNAAVEPFA
                     APVRRPEYIGARDFTPVR"
     gene            complement(6510..7577)
                     /locus_tag="KI385_RS11930"
                     /old_locus_tag="KI385_11975"
     CDS             complement(6510..7577)
                     /GO_function="GO:0010181 - FMN binding [Evidence IEA]"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1217: NADH:flavin oxidoreductase/NADH oxidase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005319060.1"
                     /locus_tag="KI385_RS11930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KI385_11975"
                     /product="alkene reductase"
                     /protein_id="WP_238572166.1"
                     /transl_table=11
                     /translation="MTTAFDPTDLAGIPLANRIVMAPMTRNRATAEGVPTPSMVRYYTQ
                     RASAGLLITEATQTELIGRGYPLTPGLHTREQIAGWREVTDSVHAAGGKIFAQLWHGGR
                     IGHPVLLPDGLVPVAPSAVAAAGQGFTHEGLKDFVTPRELTGEEVRETIAGFAAAARNA
                     IEAGFDGVEIHGANGYLVHQFLAPNSNLRTDEWGGSDEGRARFAIEVVRAVADAVGAHR
                     TGLRISPGNTFNDMTEPAPEATYTALIEAIAPLGLAYLHTCDVRDLELLARLRKRFGGT
                     FVLNPATDGRPTGLQELPLVEEGAADLLAYGALFLANPDLPARLAAGGPFNTPDRASFY
                     GGDDTGYLDYPALTD"
     misc_feature    complement(7635..7651)
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 19.66"
                     /tool="antismash"
     gene            complement(7689..8759)
                     /locus_tag="KI385_RS11935"
                     /old_locus_tag="KI385_11980"
     CDS             complement(7689..8759)
                     /NRPS_PKS="Domain: Aminotran_5 (17-350). E-value: 2.4e-17.
                     Score: 54.8. Matches aSDomain:
                     nrpspksdomains_KI385_RS11935_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018567664.1"
                     /locus_tag="KI385_RS11935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KI385_11980"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_238572167.1"
                     /sec_met_domain="Aminotran_5 (E-value: 8e-12, bitscore:
                     43.7, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQRHEPLGGAEFAPETTYLNSASSGLLPRRSAAALRAAIEESASY
                     GTMGRDYFGPPTASRAAFARLLDIPAERVAIGSSVAVQSAFVAASLPPGAEVLAPEGDF
                     SSLVNPFATHPGVKLRTVPLEALADEVRPGTALVALGAVQSLDGRIADLPAVREAARTH
                     GARTYLDITQGAGWLPLRATDFDFVVCGAFKWLLCPRGTTFMAFGGEHGDGGDGAWPAA
                     LHAGWVAGEDIGESNYGVIRPAATARRFDEPHAHFSYVAAEHSLALIEELGVATIHAHN
                     TALADHYRAGLAERGLAPRPAPGSAIVSTPGLADAERRLTEAGVRISVRGGLLRAAFHL
                     YNSTDDVDRLLALLDG"
     aSDomain        complement(7710..8708)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_KI385_RS11935_Aminotran_5.1"
                     /evalue="2.40E-17"
                     /label="KI385_RS11935_Aminotran_5.1"
                     /locus_tag="KI385_RS11935"
                     /protein_end="350"
                     /protein_start="17"
                     /score="54.8"
                     /tool="antismash"
                     /translation="YLNSASSGLLPRRSAAALRAAIEESASYGTMGRDYFGPPTASRAA
                     FARLLDIPAERVAIGSSVAVQSAFVAASLPPGAEVLAPEGDFSSLVNPFATHPGVKLRT
                     VPLEALADEVRPGTALVALGAVQSLDGRIADLPAVREAARTHGARTYLDITQGAGWLPL
                     RATDFDFVVCGAFKWLLCPRGTTFMAFGGEHGDGGDGAWPAALHAGWVAGEDIGESNYG
                     VIRPAATARRFDEPHAHFSYVAAEHSLALIEELGVATIHAHNTALADHYRAGLAERGLA
                     PRPAPGSAIVSTPGLADAERRLTEAGVRISVRGGLLRAAFHLYNSTDDVDRL"
     gene            complement(8873..9577)
                     /locus_tag="KI385_RS11940"
                     /old_locus_tag="KI385_11985"
     CDS             complement(8873..9577)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018091831.1"
                     /locus_tag="KI385_RS11940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KI385_11985"
                     /product="DsbA family oxidoreductase"
                     /protein_id="WP_238572168.1"
                     /transl_table=11
                     /translation="MRVEIWSDIACPWCYIGKARFEAGLAAFAHRDGVEVVHRSFELDP
                     KAPAATDIPVLDMLATKYGVSRAQAEEMEARVAAAAADEGLGYRSDRIHGNTFDLHRLL
                     HLGQEHGVQDALLDAFYRANFAEARTLGDPAVLSEIAVAAGLPADEVARVLADPDAYAQ
                     EVRADEREAAELGATGVPFFVIDRRYGISGGQPAEVFRQALERAHADGAVQVLSARDAE
                     VCGDDGCAVPGK"
     gene            9657..10355
                     /locus_tag="KI385_RS11945"
                     /old_locus_tag="KI385_11990"
     CDS             9657..10355
                     /EC_number="2.1.-.-"
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: oMT (37-137). E-value: 1.3e-07. Score:
                     21.9. Matches aSDomain: nrpspksdomains_KI385_RS11945_oMT.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089: methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018091833.1"
                     /locus_tag="KI385_RS11945"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KI385_11990"
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="WP_238572169.1"
                     /transl_table=11
                     /translation="MTSPQGFLGFWETTGSAKTFTHPLDPALLDAYVPRAARVLDYGCG
                     YGRLTAELAGLGYREVRGVDVSAALIARGRREHPGLDLMRWAGFPLPFEEGSFDAALLF
                     AVLTCVPGDGDQSAIVGELGRLVRPGGVLYLSDVPLQDDPYNQERYARFAQRYGTYGVF
                     ETPDGGVFRHHPPQRLRGLVRAAGFSVVTERVGSVATLDGRTAGRLQIIARREAAAASD
                     ALGAPGPAPG"
     aSDomain        9768..10067
                     /aSDomain="MT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_KI385_RS11945_oMT.1"
                     /domain_subtypes="oMT"
                     /evalue="1.30E-07"
                     /label="KI385_RS11945_oMT.1"
                     /locus_tag="KI385_RS11945"
                     /protein_end="137"
                     /protein_start="37"
                     /score="21.9"
                     /tool="antismash"
                     /translation="RVLDYGCGYGRLTAELAGLGYREVRGVDVSAALIARGRREHPGLD
                     LMRWAGFPLPFEEGSFDAALLFAVLTCVPGDGDQSAIVGELGRLVRPGGVLYLSD"
ORIGIN
        1 gcacccgcga cgagcagcag ggccgcgccg ccggcgagat acggagtggt cgaactgccg
       61 ccggtctccg cgaggttctg ggtgctcggg tgggagccgg tctgcgacac ctgggtgccc
      121 ttgccaccgg actgctcggt gccgccgccg ctcgtgccgc cgctggtctg cccaccgccg
      181 gtctggccgc cactggtctg accaccgctg gtgccgccgg agccgttgtg ctcgggcgcc
      241 ttccccgggg cggtgcaggt ggcccgggcg agggtgaccc ggccgcgtac atcggcgacg
      301 ttgagcttca gcgggttgac ggagacgttc agatcgagcg cggtggccgc ggcggtcttg
      361 gacgtgacgt tcttcttgga caggtcgagc cgcacctcgc ccacgcccgg caccttgacc
      421 tccgtcgtcc cggccgcctt gagcgtgacc ttcttgccca ggacgaggac gctgcccagc
      481 acgttcgact cggcccgggg ccggtggccc gcctcgcaca ccgccttggc ggtgacctgc
      541 tggacctcga tcagggacag cagcggcaga ccgggcagat gcaccttggc gcgcaccagg
      601 ttggcgaagc cctcggcctt gtggcggtcg gcggtggccc gtgcggtggc gacatcggcg
      661 cgcagcacgc tgaacggctt gccctggttg atgccgtcca ggtcgacggt gagcagggtc
      721 ttgtcggcgg aggccggggc gtgcacctcg ttcaggacga cgttcagcgg gacatcgagc
      781 gtcttgttga gcagaccgac gttcaggccg gtacgcagca cgaccgcacc ggacgtgccg
      841 ggggtcgagc cgtggtctcc ggtggcatgt gcgggcgcgg cgccgagcag gacggcgggg
      901 ccggccgtga gcgctgcggc gacgaaggtc gcggcgaggc gtcggggcat gccggaggag
      961 ctggtgggac tggtggacac gtgtgtggaa cccccacagg agaaatggag tcgccggccg
     1021 cgccaatggg ggacatcacg cgggccgacg acctcgaccc gcacatcgtg tctgcacgga
     1081 gggtgaacag gcagccacgt gaggtcagtt cactccaatg ggtggtttcc gcgcctctat
     1141 tcgattcccg cggcacgatg catcacccac gccacaatcg ccccaccacc tgggtgacct
     1201 gggttgaccg gcgacgttca acggctcatc gcatgagggc gccaccgttg acgtcgcctt
     1261 ccccgcggaa caggggccgg agccgcccta gccgaccacc tggccgcgca gcaccacccg
     1321 ccgcggcgcg gccagcaccc ggacgtcctc gcgtggatcg ctctcgtaga ccaccaggtc
     1381 cgccgaggcg ccctcggtga ggccggggcg gcccagccag tccctggcgc cccaggtggt
     1441 ggcggagagc gcggcgaccg ccggcagacc ggccttcgtc agctcgacga cttcctcggc
     1501 gaccaggccg tgggcgagcg agccaccggc atccgtgccg gcgaagatgg gcaccccggc
     1561 gtcgtaggcg gcccgtacgg tgtcgtagcg gcgctcgtgg agccgccgca tatggtccgc
     1621 ccagcgcggg tacttcgcct cgccaccggc cgcgagctgc ggaaacgtgg cgatgttgac
     1681 gagcgtgggg acgatggcga cgccccgctc cgcgaacagc gggatggtct cctcggtgag
     1741 tccggtggcg tgctcgatgc agtcgatacc ggcctcgacg agcggggcga gggagtcctc
     1801 ggcgaagcag tgggcggtga cccgggcgcc gagccggtgc gcctcggcga tggccgcctc
     1861 caccgcgcca gggggccagc aggccgacag atcgccggtg gagcggtcga tccagtcgcc
     1921 gacgagcttg acccagccat cgccgcgacg ggcttcccgg gcgacatagg cgaccaggtc
     1981 gtccggctcg atctcgtacg cgtagttgcg gatatagcgc ttggtacggg cgatgtggcg
     2041 gccggcgcgg atgatccgcg gcaggtcctc gcggtcgtcg atccagcgtg tgtcggaggg
     2101 ggatccggcg tcgcggatca gcagggtgcc ggcgtcacgg tcggtgagcg cctgtttttc
     2161 gctggtggga gcgtccaccg ggccgtgtgc gtccaggccg acatggcagt gggcgtcgac
     2221 caggcccggc agtgcccagc cctccacggt ggtggcatcg cgtgccatgg ccggccggtc
     2281 gtacgtcacc cggccgccga cgatccacag ctcgtcgcgc acatcgtccg gcccgacgag
     2341 gaccctcccc ttgaggtgca gcaccgcacc ttcactcatg gacgtcatac cggcacttta
     2401 ggcggcgccg cgggggccgc ggcagtggcg tcgggccgct gtgcattcgc cttccccccg
     2461 gccgtcggcc ggaagccgac gagcgtcggc cacggcttcc ccaccgcgcg ttcgcttctg
     2521 ttctccacgc gtttccgctg cgccttcccg gcgccttcgc tgtgtgttct ccgcgcgttc
     2581 tccgcacggt tctccgtacg tttccggaca atccctcgcg catgttcttg cgggtcttgt
     2641 catgggtttg ccgcacgcgt ttccatggcc cttccgccag accttctgcc ggcgaattac
     2701 caattcttca gatgtgcagc ttcccgtatt cttctgcccg cttttccgca gctcaaacgc
     2761 caagatttcg ctagtggcaa gtgccgtaat tcggacgcat ctcgacggcg ttacagagct
     2821 gtgaccgact acacagcgtg tccgtttcgc cccgaacttc ccggtcaaat cggcacatct
     2881 tgcagcctcg tcgcgcgccc gggcgccccc gcgcggtaac atatatcgat cgcttccgta
     2941 acccctgccc gcgatgcaat ttcccttttc cctgggtaaa tttaattccc atgaccgccg
     3001 cacaagcaga ccatgcacgt gcccgtagcg atatcggaga atttccggag ggcttcaagc
     3061 tcgacacccc acgcgtggag gacggagccg cgatctggcg catcgcccgc gactcgaagg
     3121 cgctggacct caactcgtcc tacagctacc tcctgtggtg tcgcgacttc gccgccacct
     3181 ctgtcgtcgc acgcgacacg gagggcgagc cggccgcctt cgtcaccggg tacatccgcc
     3241 ccgagcgccc ggagaccctc gtcgtctggc aggttgccgt cggcgaggcg caccgcggcc
     3301 ggggactggc ggccgcactc ctggacgggc tgaccacccg ggccatggac gagctgggca
     3361 ttcgcttcgt cgagaccacc gtcacccccg acaacaccgc gtccaaccgg atgttcgcgt
     3421 cgttcgcgga acggcactcg gtgcccgtga ggcgcgaggt gctcttcgat gcggggctgt
     3481 tccccgagca gggccacgag ccggaggtgc tgcacctcat aggcccgttc acaccaccgg
     3541 cccctccagg gcggtaaccc ccgagggccg cacggccaca gagccgcagc ggccgagcgg
     3601 aaccggcacc ccgtgggacc gccgtcgccg cccagccccc acagacttcc tcatcctttc
     3661 gccactcatc tcccaggagc atgctgtgac catcacccag cccgatctga gcgtcttcga
     3721 gaccgtggag tcggaggtgc gcagctactg ccgtggctgg cccaccatct tcgaccgcgc
     3781 acagggcagc cggatcaccg acgaggacgg gcacacctac ctcgacttct tctccggcgc
     3841 cggatcgctc aactacggcc acaacaaccc ggtactcaaa cgcgccctga tcgactacat
     3901 cgagcgggac ggcatcaccc acggcctgga catgtccacc acggccaagc gggcgttcct
     3961 ggagtcgttc cagaacatcg ttctgcggcc gcgcgacctg ccctacaagg tcatgttccc
     4021 gggcccgacg ggcgccaact ccgtcgaggc ggcgctgaaa ctggcccgta aggtcaaggg
     4081 ccgcgagtcg atcgtgtcct tcaccaacgc cttccacggc atgtcgctgg gcgcgctcgc
     4141 cgtcaccggc aactccatga agcgggccgg cgccggcatc ccgctggtgc acggcactcc
     4201 catgccgttc gacaactacc tcgacggggc gtacccggac ttcctgtggt tcgagcggct
     4261 gctcggggac cagggctccg gtctgaacac ccccgccgcc gtgatcgtcg agacggtcca
     4321 gggcgagggc ggcatcaatg tcgcccgccc cgagtggctg cgcgccctcg ccgacctgtg
     4381 cgagcgccgc gacatgctgc tgatcgtgga cgacatccag atgggctgcg gccgtacagg
     4441 tgccttcttc tccttcgagg aggcgggcat cgtgccggac atcgtcaccg tctcgaagtc
     4501 catcagcggc tacggcctgc cgctcgcgct gacgctgttc aagcccgagc tggacatctg
     4561 ggagccgggc gagcacaacg gcaccttccg cggcaacaac ccggccttcg tcaccgccgc
     4621 cgccgcgctc gacacctact gggccgacgg ccagatggag aagcagactc tcgcccgcgg
     4681 tgagatcgtc gaagcgcacc tgaaggccat cgtcgaggag cacccggacg ccatcgccga
     4741 gtaccgcggc cgcggtctgg tctggggcct ggagtgcatc gacaagagcc tcgcgggcaa
     4801 gatcgccacg cgcgccttcg agctgggtct gctgatcgag acctccggcc cggagagcga
     4861 ggtcgtcaag ctgctgcccg cgctgacgac cacccccgag gagctggacg agggccttcg
     4921 taccctcgcc cgcgcggtcc gcgactgcgc ctgacccccg cggggttcct taccgttcca
     4981 cagaaaggca ccgactcacc gtgatcgtcc gatccttcaa ggacatcgaa ggcaccgacc
     5041 gccacgtcaa ggccaagtcc ggtacgtggg agagcaagcg catcgtgctc gccaaggagc
     5101 gcgtcggctt ctcgctgcac gagaccatcc tgtacgcggg taccgagacg tcgatgtggt
     5161 acgcgaacca catcgaggcc gtgctgtgtg tggagggcga ggccgagctc accaacgacg
     5221 agaccggcga gaagtacacc atcacgcccg gcacgatgta cctgctcgac gggcacgaga
     5281 agcacaccat gcggatcaag gaggacttcc gctgcgtgtg tgtcttcaac ccgccggtca
     5341 ccggccgcga ggaccacgac gagaacgggg tctacccgtt gctcaccgag cccgaagcgg
     5401 agcccgaggc ggtctgaggc gtcacctcac ccgcctgccc cgtccggcag ctgaggaagg
     5461 cacgcacaca cgcacggaag ccagagcccg tacgagagga gaggaaggca acaccatgac
     5521 caccgcaccc gagcgcatcg ccgacctgta cccgacccgt gggaccgccg aggtcatcac
     5581 cccgcggaag gacccggtgg tgtggtcgca gcccggagcg gccggcccgt tcgcgccgtc
     5641 cgagctgagc gacttcgaac gcgacggctt tttcgccatc ggggaactgc tcacggcgga
     5701 ggaagtcgcg gtgtaccgcg ccgaactgga ccggctggtg ctcgacccgg cgatgcgcgc
     5761 cgacccgcgc tccatcgtcg agtcgaagtc gcagagcgta cggtccgtct tcgaggtgca
     5821 caagatcagc gaggtgttcg cccggctggt ctcggacccg cgggtggtcg gccgcgcccg
     5881 gcagatcctc ggctcggacg tctatgtcca ccagtcacgg atcaatgtga agcccggctt
     5941 cggtgcctcc ggcttctact ggcactcgga cttcgagacc tggcacgccg aggacggtct
     6001 gccgaacatg cgcaccgtgt cggtctcgat cgcgctgacg gagaactacg acaccaacgg
     6061 cggcttgatg atcatgccgg gctcgcatca gcacttcgtg ggctgcgagg gtgcgacgcc
     6121 gaaggacaac tacaagcggt ccctgcagat gcaggacgcg ggcatcccgt cggacgaggt
     6181 cctgacgaag atggcggacc ggcacggcat ccgcctgttc accggcaagg ccggctcggc
     6241 gacctggttc gactgcaacg cgatgcacgg ctccggcgac aacatcacgc cctacccgcg
     6301 cagcaatgtc ttcatcgtct tcaacagcgt ggacaacgcg gcggtggagc cgttcgcggc
     6361 tccggtccgg cgcccggagt acatcggggc gcgggacttc acgccggtga ggtagacacg
     6421 gcggagacac cggtggcgcg gtcgccgcac agcagggtct gtgcggcgac cgcgccgtct
     6481 cgtgcgagga ggcgtttcgg gagtacggct cagtccgtca gcgccgggta gtcgaggtag
     6541 ccggtgtcgt cgccgccgta gaagcttgcg cggtccgggg tgttgaacgg gccgccggcc
     6601 gccagccgcg cgggcagatc ggggttggcg aggaacagcg cgccgtacgc gagcagatcg
     6661 gccgcaccct cctcgacgag cgggagctcc tggaggccgg tgggccggcc gtcggtggcc
     6721 gggttgagca cgaaggtgcc gccgaagcgc ttgcgcagcc tggccagcag ctccaggtcg
     6781 cgcacatcgc aggtgtgcag gtaggccagg cccagcgggg cgatggcctc gatcagcgcg
     6841 gtgtaggtgg cttcgggtgc gggttcggtc atgtcgttga aggtgttgcc gggcgagatg
     6901 cgcagaccgg tgcggtgcgc gccgaccgcg tcggcgacgg ccctgacgac ctcgatggcg
     6961 aagcgggcgc ggccctcgtc ggagccgccc cactcgtcgg tacgcaggtt ggagttgggc
     7021 gccaggaact ggtggaccag atagccgttg gcgccgtgga tctccacgcc gtcgaacccg
     7081 gcctcgatcg cgttacgggc cgcggccgcg aacccggcga tggtctcgcg tacctcctcg
     7141 ccggtcagct cgcgcggggt gacgaagtcc ttgaggccct cgtgggtgaa gccctggccg
     7201 gccgcggcga ccgcggaggg cgcgaccggc accaggccgt cgggcagcag caccgggtgg
     7261 ccgatgcggc cgccgtgcca cagctgggcg aagatcttgc cgcccgcggc gtgcacggag
     7321 tcggtgacct cgcgccagcc ggcgatctgc tcgcgggtgt gcaggccggg ggtcagtggg
     7381 tagccgcgac cgatgagttc ggtctgggtg gcctcggtga tgaggagacc ggccgaggcc
     7441 cgctgggtgt agtagcggac catggacggg gtggggacgc cctcggcggt ggcgcggttg
     7501 cgggtcatgg gcgccatgac gatgcggttg gcgaggggga tgccggcgag gtcggtgggg
     7561 tcgaacgcgg tggtcactga cggcctctca actgtggtca acgggtcatc aactcaggta
     7621 cggagctgaa tcgttggcca ttacatcagc aaccaccgcg accccggcat tcccgccgcg
     7681 ccgggaccct aaccgtcgag cagggccagc agccggtcca cgtcatccgt cgagttgtag
     7741 agatggaagg ccgcgcggag cagcccgccg cgcaccgaga tccgtactcc cgcctcggtc
     7801 agccggcgct ccgcgtccgc gagcccgggg gtggagacga tcgccgagcc gggcgcggga
     7861 cggggcgcga ggccgcgctc ggccagcccc gcgcggtagt ggtcggcgag cgcggtgttg
     7921 tgggcgtgaa tcgtggccac gcccagctcc tcgatgaggg cgagggagtg ctcggcggcg
     7981 acgtaggaga agtgggcatg cggttcgtcg aagcgccggg cggtggcggc cggccggatc
     8041 acgccgtagt tggactcgcc gatgtcctct ccggcgaccc agcccgcatg caacgcggcc
     8101 ggccatgccc cgtcgccgcc gtcgccgtgc tccccgccga aggccatgaa ggtcgtaccg
     8161 cgcgggcaca gcagccattt gaaggcgccg cagacgacga agtcgaagtc ggtggcgcgc
     8221 agcggcagcc agccggcgcc ctgggtgatg tcgagatacg tacgcgcccc atgggtccgc
     8281 gcggcctccc gtaccgccgg cagatcggcg atccggccgt cgagcgactg cacggcgccg
     8341 agggcgacga gggcggtgcc ggggcgcacc tcgtcggcga gcgcctccag cggtacggtg
     8401 cgcagcttga cgcccgggtg ggtggcgaac ggattgacca gggagctgaa gtcgccctcg
     8461 ggggccagca cttcggcgcc cggcgggagc gaggcggcga cgaacgcgga ctgcaccgcg
     8521 accgagctgc cgatggcgac ccgttccgcg gggatgtcca gcagccgggc gaaggcggcc
     8581 cgggaggcgg tggggggtcc gaagtagtcg cggcccatgg tgccgtagga ggcggattcc
     8641 tcgatggccg cgcgcagggc ggcggcgctg cgccggggga ggagtccgct ggaggcggag
     8701 ttcagatagg tcgtctcggg cgcgaattcg gccccgccca gtggctcatg tcgctgcatg
     8761 actccactgt gcggggcgat ccgcgccccg tccacggcgt atccgccggg ccggcccgca
     8821 agcatggctc atccgtgcag gtcgcagggc atgcgggcgg gccggggagc ggtcacttcc
     8881 cgggaacggc gcagccgtcg tccccgcaga cctcggcgtc gcgggccgac aggacttgca
     8941 cggcaccgtc ggcgtgggcg cgctccaggg cctgccggaa gacctcggcg ggctggccgc
     9001 cggagatgcc gtaccggcgg tcgatgacga agaagggcac gccggtggcg ccgagctcgg
     9061 cggcctcgcg ctcatcggcg cgtacctcct gggcgtacgc gtcggggtcg gccagcaccc
     9121 gggcgacctc gtcggcgggc aggccggcgg cgacggcgat ctcgctgagc accgcggggt
     9181 cgccgagggt cctggcctcg gcgaagttgg cgcggtagaa ggcgtcgagc agcgcgtcct
     9241 ggacgccgtg ctcctggccg aggtgcagca gccggtgcag atcgaaggtg ttgccgtgga
     9301 tccggtcgga gcggtagccg agaccctcgt cggcggccgc ggcggcgacc cgggcctcca
     9361 tctcctcggc ctgcgcgcgg ctgacgccgt acttcgtggc gagcatgtcg agcaccggta
     9421 tgtcggtggc ggccggtgcc ttcgggtcca gctcgaagga gcggtgcacc acctcgacac
     9481 cgtcgcggtg cgcgaaggcg gcgagcccgg cctcgaaacg ggccttgccg atgtagcacc
     9541 aggggcaggc gatgtcggac cagatttcga cgcgcatgag ctgactttcc tcgtggtgga
     9601 tgtgtcctgg tgcaacctcg tggggcggcg ggctattccg cgggtacggt ccgggcatga
     9661 cctcgccaca gggatttctc ggcttctggg agacgaccgg gtccgccaag accttcaccc
     9721 atcccctgga tccggcgctg ctggacgcct atgtgccaag ggccgcgcgg gtgctggact
     9781 acggctgcgg ctacgggcgg ctgaccgccg agctggccgg gctcgggtat cgcgaggtgc
     9841 gcggggtgga tgtctcggcg gcgctgatcg cgcgggggcg gcgcgagcat ccggggctgg
     9901 atctgatgcg gtgggccggg tttccgctgc cgttcgagga ggggtcgttc gatgcggcgc
     9961 tgctgttcgc ggtgctcaca tgtgtgccgg gggacgggga tcagtcggcg atcgtcggcg
    10021 agctggggcg gctggtgcgc cctggcgggg tgctgtatct gagcgatgtg ccgttgcagg
    10081 acgatccgta caaccaggag cgctatgcgc ggttcgcgca gcggtacggc acgtacgggg
    10141 tgttcgagac gcccgacggc ggggtgttcc ggcaccatcc gccacagcgg ctgcggggcc
    10201 tggtgcgggc ggcgggcttc tcggtggtga cggagcgggt ggggtcggtc gccacgctgg
    10261 acgggcggac ggccgggcgg ctgcagatca tcgcccggcg ggaggcggca gcggcgtcgg
    10321 atgctcttgg ggcgccaggt ccagcgccag ggtgaggtgg gtggcgtcgg gggcggggcg
    10381 gcggtccggg tcggggcgcc agccgaggcc ggtgtag
//
