LOCUS       NZ_CP084681            10216 bp    DNA     linear   CON 30-APR-2024
DEFINITION  Streptomyces inhibens strain NRRL30439 chromosome, complete genome.
ACCESSION   NZ_CP084681
VERSION     NZ_CP084681.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces inhibens
  ORGANISM  Streptomyces inhibens
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 9696490)
  AUTHORS   Barry,C.P., Gillane,R., Talbo,G.H., Plan,M., Palfreyman,R.,
            Haber-Stuk,A.K., Power,J., Nielsen,L.K. and Marcellin,E.
  TITLE     Multi-omic characterisation of Streptomyces hygroscopicus NRRL
            30439: detailed assessment of its secondary metabolic potential
  JOURNAL   Mol Omics 18 (3), 226-236 (2022)
   PUBMED   34989730
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9696490)
  AUTHORS   Barry,C.P., Palfreyman,R. and Marcellin,E.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-OCT-2021) EMS, Australian Institute for Bioengineering
            and Nanotechnology, Cnr College Rd & Cooper Rd, Brisbane, Queensland
            4103, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. APRIL-2018
            Assembly Name                     :: AIBN_shyg
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 239.0x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022221585.1-RS_2024_04_30
            Annotation Date                   :: 04/30/2024 04:58:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,775
            CDSs (total)                      :: 8,682
            Genes (coding)                    :: 8,305
            CDSs (with protein)               :: 8,305
            Genes (RNA)                       :: 93
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 158 of 377
            Pseudo Genes (incomplete)         :: 310 of 377
            Pseudo Genes (internal stop)      :: 38 of 377
            Pseudo Genes (multiple problems)  :: 116 of 377
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 23:32:03
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1288762
            Orig. end                         :: 1298978
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP084681.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Australian Institute for
            Bioengineering and Nanotechnology, Building 75,Cnr College Rd &
            Cooper Rd, Brisbane City QLD 4072.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10216
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="10"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10216
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10216
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5216](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5216)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(51..63)
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 19.46"
                     /tool="antismash"
     misc_feature    580..593
                     /note="TFBS match to RpoN, Regulator of nitrogen
                     assimilation and virulence, confidence: strong, score:
                     17.22"
                     /tool="antismash"
     misc_feature    complement(769..786)
                     /note="TFBS match to HipB, Bacterial antitoxin HipB,
                     confidence: weak, score: 20.42"
                     /tool="antismash"
     misc_feature    complement(775..789)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 17.3"
                     /tool="antismash"
     gene            complement(1724..4018)
                     /locus_tag="KI385_RS06075"
                     /old_locus_tag="KI385_06120"
     CDS             complement(1724..4018)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018091742.1"
                     /locus_tag="KI385_RS06075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KI385_06120"
                     /product="NHLP family bacteriocin export ABC transporter
                     peptidase/permease/ATPase subunit"
                     /protein_id="WP_238571194.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 2.7e-37, bitscore:
                     126.5, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTAPHDSAAAQQQQLPPPVRGRHRPEPAPGARRRTRRDAPPAQKP
                     KKAKAVRTPTVLQMEAVECGAASLAMVLAHFGKHVPLEELRIACGVSRDGSRASNLLKA
                     ARSYGLQAKGMQMEPAALAEVQAPAILFWEFNHYVVHDGMGRRFGRRGVHINDPDKGRR
                     FVSMEDFDTSFTGVALVLEPGESFRKGGRKPGVLSAVPARMRGTKGTLLAALLASLLLV
                     AVGAAVPALSRTYIDMFLIGNQTSLLGPLFASMAAMVALTAVLTGLQQANLLRGRIISS
                     TLTSARFLRHLLRLPVTFFAQRSPADLVQRLQSNDAVAETLARDLAAAGVDGIVVILYA
                     LLLWTYDPQLTVIGVGIALLNVVAMRIVIRLRATHTQKLRADTARLTNTSYTGLQLIET
                     MKATGGENGYFRRWAGQHATTLEEQQRLGVPSAALAVVAPTLATLNSALILWIGGLRAV
                     EGHISIGLLVAFQALVTRFTAPITRLNGVAGRIQDFAADVARLKDVESFPVDTLYSRPE
                     PDASTRRLKGHVTLEDITFGYSPLDKPLLTGFSLAVGPGRQVALVGGSGSGKSTVSRLI
                     SGLYSPWEGTIRIDGQRLEDLSRSALAASVSFVDQDIFLFEGTVRDNVALWDPSIPDDA
                     VIAALQDAALYDDVIARRPDGIHSRVEQDGRNFSGGQRQRLEIARALVRRPSILVLDEV
                     TSALDAQTEQTIMDNLRRRGCACVVIAHRLSTVRDSDEIVVLDHGVVVERGRHEDLVAA
                     GGPYAELVKEH"
     gene            complement(4015..4821)
                     /locus_tag="KI385_RS06080"
                     /old_locus_tag="KI385_06125"
     CDS             complement(4015..4821)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007386622.1"
                     /locus_tag="KI385_RS06080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KI385_06125"
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="WP_238571195.1"
                     /transl_table=11
                     /translation="MQFRQQALSKLQSPEEIDLPVRFARPQGWLVLTITVLVVIAASVW
                     AVTGTVSSTLSAPGILTHGQGSYILQSPVAGQVTAVLAEEGTQVAANTPLLKVRTAQGT
                     TVVRTLAAGRLTTMFATIGSVVTTGADVASVERVAHAGDPLTATLYVPAESGASVPVGA
                     SVDLTVQSVPAQQYGVLRGKVKAVGRTAQTRQRIGAFLGSSQLGEQFSQHGQPVAVLVQ
                     LDRDPHSRSGYSWSTSDGPPYAVDSMTLAGGAVHLAAQHPIDWLLP"
     misc_feature    complement(4902..4921)
                     /note="TFBS match to DosR, Hypoxia stress response
                     regulator, confidence: weak, score: 23.34"
                     /tool="antismash"
     misc_feature    4947..4959
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 19.5"
                     /tool="antismash"
     gene            complement(5001..5216)
                     /locus_tag="KI385_RS06085"
                     /old_locus_tag="KI385_06130"
     CDS             complement(5001..5216)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: strepbact"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020128984.1"
                     /locus_tag="KI385_RS06085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KI385_06130"
                     /product="type A2 lantipeptide"
                     /protein_id="WP_128504877.1"
                     /sec_met_domain="strepbact (E-value: 1e-33, bitscore:
                     114.6, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNSTPQVQTQEISDSDLDNVSGGLVGNVLGTVTGTVDSVAPVSGV
                     VGTATGLVAQTTGVNTSGVTGLVAGL"
     misc_feature    5236..5249
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 15.94"
                     /tool="antismash"
     misc_feature    5448..5463
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 19.06"
                     /tool="antismash"
     misc_feature    complement(5448..5465)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 17.72"
                     /tool="antismash"
     misc_feature    complement(5589..5607)
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 19.54"
                     /tool="antismash"
     misc_feature    5621..5630
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 14.08"
                     /tool="antismash"
     misc_feature    5691..5708
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 14.87"
                     /tool="antismash"
     gene            5961..6992
                     /locus_tag="KI385_RS06090"
                     /old_locus_tag="KI385_06135"
     CDS             5961..6992
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018091739.1"
                     /locus_tag="KI385_RS06090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KI385_06135"
                     /product="PHP domain-containing protein"
                     /protein_id="WP_238571196.1"
                     /transl_table=11
                     /translation="MDPVEALERIAFLLERQGAVTYRVQAFRTAAAAIGALSPDELQKR
                     AHDGSLGRLKGLGPKTARVVEEALAEVRPEYLAHLEDEAGGPLVEGERGARLRRALRGD
                     CHLHSDWSDGGSPVATMARTARGLGHKWCVLTDHSPRLTVARGLTAERLRQQLSLVAEV
                     NERLAPFRLLTGIECDILDDGALDQEPELLEQLDVVVASVHSKLRMERAPMTRRLLAAV
                     RNPLVDVLGHCTGRQITGKLRPESQFDAGEVFAACAEHHTAVEINCRPDRFDPPRRLLR
                     QALETGTLFSIDSDAHAPGQLDWQIYGCARAEECGVPADRVINTWTERQLATWTRTGRT
                     PRR"
     gene            complement(7002..8348)
                     /locus_tag="KI385_RS06095"
                     /old_locus_tag="KI385_06140"
     CDS             complement(7002..8348)
                     /GO_function="GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /GO_process="GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018091737.1"
                     /locus_tag="KI385_RS06095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KI385_06140"
                     /product="chloride channel protein"
                     /protein_id="WP_238571197.1"
                     /transl_table=11
                     /translation="MSVGPTGPVGGAPATVPEDPYAMVRTGGYLKLLLVATVIGVPVCA
                     ASFGFLTLVHALEPLINEDLPRALGFAGTPLWWPLPMLAVAGLLVGLTIRYLPGNGGHE
                     PADGLTVGGAPALSALPGIVLAALASLSFGAVLGPEAPLIALGGGLAVGAVRLMNLNMP
                     GRAVATVGAAGSFAAVSALLGSPLLGAFLLMEVSGLSGPMLGMMLVPGLLASGIGALIF
                     IGLGNWTGLGTYSLALHDIPRATEPTLAEFGWALVIGVAAAVVASGVRRLSLFLKGRVV
                     RHRVPATVVMGLVVGALAVGFAEATGKPATEMLYSGQSALGSLFRDSAGYSVAALLLLV
                     LCKGLAYCASLSAFRGGPIFPALFIGAAGGVALSHLPGLSLTPGFAMGVGAMCVAMLRL
                     PMTSVLLATLLMGYNGLTVMPLVIVAVVVSYVVALRLTPAAPATPPAAG"
     gene            complement(8448..9356)
                     /locus_tag="KI385_RS06100"
                     /old_locus_tag="KI385_06145"
     CDS             complement(8448..9356)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1014: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018091736.1"
                     /locus_tag="KI385_RS06100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KI385_06145"
                     /product="LysR family transcriptional regulator"
                     /protein_id="WP_238571198.1"
                     /transl_table=11
                     /translation="MIEARRLHILRAVADHRTVTAAAAALYLTPSAVSQQLAALEQETG
                     HRLVERSARGVRLTAAGDILLTHANAVLAQLERAESELAAYGSGEAGTVTVAAFATGIG
                     LVVAPALTALARSAPGIRVRVQDAEGDASLRMVLDRQVDVAVAVEYRGAPGADDPRLTR
                     VPLYAEPFDAVLPPGHRLADAPQVAVADLAGDLWIGPYPGNPCHDVVVLACEYAGFQPR
                     LEHSSDDFRAVVALASAAAGVALVPRSALRDMDLTGVVVRPVDDVAPTRKVFAAVRRGA
                     EEHPLFRPVLAALQEAAAPQG"
     misc_feature    complement(9321..9337)
                     /note="TFBS match to NtrC, Nitrogen regulatory protein C,
                     confidence: weak, score: 21.67"
                     /tool="antismash"
ORIGIN
        1 catgacgagg aagaccgccc ctatgacgac cagcatgccg accgccgcca gtgccaacgg
       61 cacgctgtag cagagcagca gcacaaggtt catcgcgccg atcgtggtgg actgcacggc
      121 caccgggccg atgccggaga gcacccggcg gatggcgctg atgcccatcg cggcgctggc
      181 cagctctccg gtggagcgtt cggtgaagaa cttcgtcggc agccgcagca gccggtccca
      241 tacggccggt tgcagagcgc tctcgatgcg gccctccatc cgcagcacgg tgaggttctg
      301 cagcagcatg aaggcggcgg agacgacgct ggtgatgatg acggccaggg agacctggac
      361 gatcaggctc cgctcggcgc tcggtacgta gaccccgagc accttgccgg tcgcgatcgg
      421 gacgagcgcg ccgaggccga ccgtcaccag tccgctgagg gccagattgc gtacgtccat
      481 acgggtgccg cgcaggctga agcgcatcag ccgccacatg ctcatgggcc gctcgggcag
      541 cgggcggtag aacatcaccg cgcgcggctc gaaggcgtcg gcattgtcct tgccgatgcg
      601 gatccgcagc cccgaggccg ggttgaccgc ctcgtagcgg ccacggcgcc acagcagcgc
      661 caccggggcg ccggacttgg cgcgatggcc caccaggggg ccggtgtcgg tccgccacca
      721 gcgcccgacg agccggaccg cgcgggtgcg gatccgcgag ctgaccgcga tccgctcgac
      781 gggagtgatg cggtcgttga cggcgccgcc cttggggggc tcggtcagcg tgatgccggc
      841 cgcctcggcg accgcgcggc agaccgcgaa ggtggtgtcg tcgccggccc ggtccgtccc
      901 cgcggacgcc cgcccggacc ggtcctgacg gccgatggag gccagcagcg cctggtcggc
      961 gcgctcgcga acggtctcgc ccgccttgat gccggccgcc gtgcggtcct cgtgagcgcg
     1021 ctccagctgc tcgatccagc ggtcgacggc ggacagcagc cggtactgct ggttgaccat
     1081 ccgctgccag agcgcggggt ccatcagcag atcgccggcc gcctccgcgc tgtaggaggc
     1141 gccgtactgc acactgccgg gcgggaccgg catccacagg acgtcgtcgt cggccaccgc
     1201 gtcgtcgccg ggccggccgt ccaacggtgc ctcgaacagg acgcccagac tgcgtgcggt
     1261 gcccagcgca acggcgtgct ccagcgggga gggcgccatc ccgccctggc ccgggtgggc
     1321 ggggtcgtgg tcgtagccgg tctgcgcgcc gtactgcgca tgggccccgg tgtcccactg
     1381 atcgccgtag tcgtaccggg gcagctcgcg cagcgggatc cggcgcagcc ggcagtcctg
     1441 cgagggccgg ccgagcaggg tgtgctgtgg gccctcgacc gggccgagca gcagggtgcc
     1501 cgcttcgagc cggccgagga agtgccagtg cccctcgtgg acggcgtcga ccgcgaagag
     1561 atccagcgtc ccgcccgtga cgagccacag cacatgcggc ccttccaggg ggacgctgcg
     1621 cagcccggcg cagtcgacgg gcgagcccag gccgcccagc gcctgcgtca ccgcgtcggt
     1681 ctcgcccggt cccacgaccg gggacgggga tacggatgac acgtcagtgc tccttgacca
     1741 gctcggcgta cgggcccccg gcggcgacca gatcctcgtg ccggccgcgt tcgacgacca
     1801 cgccgtggtc gaggaccacg atctcgtcgc tgtcgcggac ggtgctcagc cggtgggcga
     1861 tgacgacaca ggcgcagccg cgccgccgca ggttgtccat gatggtctgc tcggtctggg
     1921 cgtccagtgc gctggtgacc tcgtcgagga ccaggatgct ggggcgccgg accagtgcgc
     1981 gggcgatctc cagccgctgg cgctgtccgc cggagaagtt gcggccgtcc tgctcgacgc
     2041 ggctgtggat gccgtcgggg cgacgggcga tcacatcgtc gtagagcgcg gcgtcctgga
     2101 gagcggcgat gaccgcgtcg tccggtatcg acgggtccca cagcgccacg ttgtcacgga
     2161 ccgtgccctc gaacaggaag atgtcctggt cgacgaagga gacggaggcg gccagcgcac
     2221 tgcgggagag gtcctccagg cgctgtccgt cgatgcggat ggtgccttcc caggggctgt
     2281 agaggccgga gatcagccgg gagacggtgg acttgccgct gccggagccg ccgacgaggg
     2341 cgacctgccg accggggccg acggccaggg agaagccggt cagcagcggt ttgtccaggg
     2401 ggctgtagcc gaaggtgatg tcttccagcg tcacatggcc cttgagcctg cgggtgctgg
     2461 catccggctc cgggcgtgag tagagggtgt cgacggggaa gctttcgacg tccttgaggc
     2521 gggccacgtc ggcggcgaag tcctggattc tgcccgccac tccgttgagc cgggtgatgg
     2581 gcgcggtgaa gcgggtcacc agcgcctgga aggcgacgag cagaccgatg gagatatggc
     2641 cttcgaccgc ccggagtccg ccgatccaca ggatcagcgc gctgttgagc gtggcgaggg
     2701 tgggggcgac gacggccagc gcggcgctgg gcacgccgag ccgctgctgt tcctccagcg
     2761 tggtggcgtg ctgcccggcc cagcggcgga agtagccgtt ctccccgccg gtggccttca
     2821 tcgtctcgat gagctgcagg ccggtgtagg aggtgttggt cagccgggcg gtgtcggcgc
     2881 gcagcttctg ggtgtgggtg gcgcgcagcc ggatcacgat ccgcatcgcg accacgttga
     2941 gcagggcgat ccccacgccg atgaccgtca gttgggggtc gtaggtccac agcaggaggg
     3001 cgtagaggat gacgacgatc ccgtcgacac ccgccgcggc gaggtcgcgt gccagggttt
     3061 cggcgaccgc gtcgttggac tgcagtcgct ggaccaggtc ggccgggctg cgttgggcga
     3121 agaaggtgac cgggagtctg aggagatggc gcaggaaccg ggcgctggtg agggtggagg
     3181 agatgatccg gccgcgcagc aggttcgcct gttgcagtcc ggtcagcacg gcggtaagcg
     3241 ccaccatcgc ggccatcgac gcaaagagcg gtcccagcag cgaggtctgg ttgccgatca
     3301 ggaacatgtc gatatacgta cggctcagcg cgggcaccgc cgcgccgacc gcgaccagca
     3361 gcaggctggc gagcagcgcg gccagcaggg tgcccttcgt gccgcgcatc cgggccggca
     3421 cggcgctcag cacgccgggc ttgcggccgc ccttgcggaa ggattcgccg ggctccagga
     3481 ccagggcgac accggtgaag ctggtgtcga agtcctccat cgagacgaag cggcggccct
     3541 tgtcggggtc gttgatgtgt acaccgcggc ggccgaagcg gcggcccatg ccgtcgtgga
     3601 cgacatagtg gttgaactcc cagaacagga tggccggggc ctgcacctcg gcgagcgcgg
     3661 ccggttccat ctgcatgcct ttggcctgca ggccgtagct gcgggcggcc ttgagcagat
     3721 tgctggcgcg cgagccgtcg cgggagacgc cgcaggcgat ccgcagctct tcgagcggca
     3781 catgcttgcc gaagtgggcg agcaccatgg ccagggacgc ggcgccgcat tccacggcct
     3841 ccatctggag gacggtgggg gtgcggacgg ccttcgcctt cttgggcttt tgggcagggg
     3901 gagcgtcacg gcgggtgcgg cgacgggcgc cgggcgcggg ctccgggcgg tgccggccgc
     3961 ggacgggcgg cggcagttgc tgctgctgtg ctgcggcgga gtcgtggggt gcggtcacgg
     4021 cagcagccaa tcgatcgggt gctgcgcggc caagtggacg gcgccgccgg ccagggtcat
     4081 ggagtcgacg gcgtacggcg ggccgtcgga ggtcgaccag gagtagccgg acctgctgtg
     4141 cgggtcgcgg tcgagctgta ccaggaccgc cacgggctgg ccgtgctgcg agaactgttc
     4201 gccgagctgg ctgctgccca ggaaggcgcc gatgcgctgg cgggtctggg cggtccggcc
     4261 gacggccttg accttgccgc gcagtacgcc gtactgctgg gccggcacgg actggacggt
     4321 gaggtcgacg gacgcgccca cggggaccga ggcgccgctc tcggccggta cgtacagcgt
     4381 cgccgtcagc ggatcgccgg cgtgcgcgac gcgttccacg gacgcgacat ccgctccggt
     4441 cgtgacgacg gagccgatcg tggcgaacat cgtggtcagc cggccggcgg cgagggtgcg
     4501 gacgacggtg gtgccctgtg ccgtacggac cttcaggagg ggggtgttgg cggccacctg
     4561 tgtgccttct tcggcgagta cggcggtgac ctggcccgcg acggggctct gcaggatgta
     4621 gctgccctgg ccgtgggtga ggattccggg ggcgctgagg gtggacgaca cggtgccggt
     4681 caccgcccac acggatgcgg cgatcacgac gaggaccgtg atggtcagca cgagccagcc
     4741 ctggggccgg gcgaatcgca ccggcagatc tatttcctcg ggcgactgca gcttggaaag
     4801 ggcctgttgg cgaaactgca cgacactctt ccctcaacgg caatggtctg tttcagacgg
     4861 gtggcggccg agtgccccgg ggcccggggg gaccgggtgg gcccggggca ctcggcccgg
     4921 catgtcggca aggagggggc gccgacctgc cgttgtcacc gttcaaagcc ggctgtgccg
     4981 tgtggcggca cagagggggc tcagagaccg gcgaccaggc ccgtgacgcc cgaggtgttg
     5041 acaccggtgg tctgggcgac caggccggtg gcggtgccca cgacaccgga gaccggggcg
     5101 acggagtcaa cggtgccggt cacggtgccg agcacgttgc cgacgaggcc accggacacg
     5161 ttgtccaggt cgctgtcgga gatttcctgg gtctgaacct ggggggtgga gttcatgatg
     5221 tttcccttcg gatgaatatc tcgaaaaaag ccgcgcccga accgtccggt gggtggtccg
     5281 gccgcggcag cgggagtcgg gtgcccgaaa tcgggaaccg ttggtgtgtc tctcgcagtg
     5341 tgaaggatca aagcatgcgg gcgcgttgtg ctgctaatcc gaccggtgct gatcgggaca
     5401 tcatcggcct gccaaattac ctgacgtgca cgcgtgtcca tgaatgagta acagcttctt
     5461 cacatcttcc cccgtttcct ttccgggggg ccgtcgtacg caccgcaggc gaacgggaca
     5521 ttcccgcatc aaaccgctta ggcgcaggtc agcgcgggga agcgacacgg aaggccgtgt
     5581 gccgactgcg cattcagtgt gcagattctt tgaagaccgc cgatttttgc ggttcgttga
     5641 cacggggtgg acggcgaact ccggcacacg ctcccgacgg agcccgccga atgttgcacc
     5701 ggcattaacc atgaccgatt cgcccggact tcatgggcgg cattgcaggt gttgagggta
     5761 ttcaacggaa tcatcggcac tatccgtacg ccattcccct ggacacggat cgaccggcgg
     5821 tagaacggac ataaccgcat aacaacgcgg gcttcggacc cgccagaaat ggccgattta
     5881 ccgccccggt ggcagccgct caccggctgg ggcacggtga aaggagaccg cgggaccggc
     5941 ggaccgggaa ggagccgtac gtggaccccg tcgaggcgct ggagcggatc gcctttctgc
     6001 tggagcgaca gggggcggtg acctaccgcg tacaggcgtt ccgtacggcc gcggcggcga
     6061 tcggcgcgct ctcccccgac gagctgcaaa agcgcgccca cgacggttcg ctggggcggc
     6121 tcaaagggct ggggcccaag accgcccgag tggtcgagga ggcgctcgcg gaggtgcggc
     6181 ccgagtatct ggcacatctg gaggacgagg cgggcgggcc gctggtcgaa ggggagcggg
     6241 gagcgcggct gcggcgggcg ctgcgcggcg actgccatct gcactcggac tggtcggacg
     6301 gcggcagccc ggtcgcgacg atggccagga ccgcccgcgg cctggggcac aagtggtgtg
     6361 tgctgaccga ccactccccc agactcaccg tcgcccgcgg actgaccgcc gagcggctgc
     6421 ggcagcaact gtcgctggtg gccgaggtga acgaacggct ggcgccgttc cggctgctga
     6481 ccgggatcga gtgcgacatc ctcgacgacg gcgcgctcga ccaggagccg gagctgttgg
     6541 agcagctcga tgtggtggtg gcatcggtgc actccaaact gcggatggag agggcaccga
     6601 tgacccggcg gctgctggcc gcggtccgca atccgctcgt cgatgtgctc gggcactgca
     6661 ccgggcggca gatcaccggc aagctccgcc cggagtcgca gttcgacgcc ggggaggtgt
     6721 tcgcggcctg cgccgaacac cacaccgcgg tggagatcaa ctgtcgtccg gaccgcttcg
     6781 atccgccacg ccggctgctg cgccaggcgc tggagacggg gaccctcttc tcgatcgaca
     6841 gcgatgcgca tgctccgggc caactcgact ggcagatcta cggctgtgcc cgcgccgagg
     6901 agtgcggtgt gccggcagac cgggtcatca acacctggac ggagcgccag ttggccacct
     6961 ggacccgtac gggccggacg ccgcgccgct gaacgggccg gtcacccggc ggccggcggg
     7021 gtcgcggggg cggccggggt gagccgcagc gccacgacgt aggagacgac cacggcgacg
     7081 atcaccagcg gcatgacggt gagaccgttg tagcccatca gcaacgtggc caacagcacc
     7141 gacgtcatcg ggagcctgag catcgcgacg cacatcgccc cgacgcccat cgcgaagccc
     7201 ggggtgagcg agagaccggg cagatgcgag agcgccacac cgcccgcagc cccgatgaac
     7261 agcgccggga agatggggcc gccccggaag gcgctcagcg aagcgcagta cgccaggccc
     7321 ttgcacagga cgaggagcag cagcgccgcc accgaatagc cggcgctgtc acggaagagc
     7381 gaacccagcg ccgactggcc cgaatagagc atttcggtcg cgggcttgcc ggtcgcctcg
     7441 gcgaaaccga ccgccagcgc ccccaccacc aggcccatca ccaccgtcgc cggtacccgg
     7501 tgacggacca cccgcccctt gaggaacagc gagagccgcc gtacaccgga cgcgacgacc
     7561 gcggcagcca ccccgatgac cagcgcccag ccgaactccg cgagcgtcgg ctccgtggcc
     7621 cgggggatgt cgtgcagtgc gagcgagtac gtaccgaggc cggtccagtt ccccagcccg
     7681 atgaagatca gcgcaccgat gccggacgcc agcagaccgg gcaccagcat catcccgagc
     7741 atgggaccgc tcagccccga gacctccatc agcaggaacg cgcccaggag cggggagccg
     7801 agcagcgcgc tgaccgccgc gaaactgccg gcggcgccca ccgtggcgac ggcccggccc
     7861 ggcatgttca ggttcatcag ccgtacggcg cccacggcca gtccaccgcc gagggcgatg
     7921 agcggcgcct cggggccgag caccgcgccg aagctgaggg aggccagggc cgcgagcacg
     7981 atcccgggga gcgccgagag ggcgggcgcg ccgccgaccg tcagcccgtc ggccggctca
     8041 tggccaccgt tgccggggag gtaacggatg gtcagcccga ccaggagacc ggccactgcg
     8101 agcatcggca gcggccacca cagcggcgtc ccggcgaagc cgagcgcccg cggcaggtcc
     8161 tcattgatca gcggttcgag tgcatggacc agcgtgagaa agccgaacga ggcggcacag
     8221 accgggaccc cgatcaccgt cgccaccagc agaagcttca gatacccgcc ggtgcgcacc
     8281 atcgcgtacg gatcctcggg cacggtggcc ggtgctccgc cgaccgggcc ggtggggccg
     8341 accgacatcg cctcgcctcc tcaccgccgc cgccgtgacg agcctgccat gaccggctcc
     8401 ggtcggcacc tcggacacga tccggacgag cggcgttcct gcgcccctca cccctgaggc
     8461 gccgccgctt cctggagggc ggcaaggacc gggcggaaga gcgggtgctc ctcggctccg
     8521 cggcgtaccg cggcgaagac cttgcgggtg ggggccacat cgtcgacggg gcggaccacc
     8581 acccccgtga ggtccatgtc gcgcagggcc gagcggggga cgagggccac gcccgccgct
     8641 gcggaggcga gggcgacgac ggcgcggaag tcgtcggagg agtgttccag gcggggctgg
     8701 aagccggcgt attcgcaggc caggacgacc acgtcatggc aggggttgcc ggggtagggg
     8761 ccgatccaga ggtcgcccgc gaggtcggcc acggccacct gtggcgcgtc ggcgaggcgg
     8821 tggccgggcg ggagtacggc gtcgaacggc tcggcgtaga gcgggacgcg ggtcagccgt
     8881 gggtcgtccg cgcccggtgc gccccggtat tcgacggcca ccgcgacatc gacctgccgg
     8941 tcgaggacca tccgcaggct ggcgtcgccc tcggcgtcct ggacacggac ccggatgccg
     9001 ggcgcggagc gggcgagcgc ggtgagggcg ggcgcgacga ccaggccgat gccggtggcg
     9061 aaggccgcga cggtcaccgt gcccgcctcg cccgagccgt aggcggcgag ctcggactcg
     9121 gcgcgctcca gctgtgccag gacggcgttg gcgtgggtga gcaggatgtc gcccgcggcg
     9181 gtcagccgta caccgcgggc gctgcgctcc acaaggcggt ggccggtctc ctgttccagg
     9241 gcggccagct gctgggagac cgccgagggc gtgagataga gcgcggcggc cgcggcggtg
     9301 accgtgcggt ggtcggccac cgcacggagg atgtgcagcc gtcgtgcttc gatcatgttt
     9361 ccagtttcgc agcagtgacg ctcgggtcgc gtcactgccc cagcgcggac ctcgcatcga
     9421 tgaacgcgtc gaccgcgcgg ttgacgtcct cggtggagtg ggccgcggac agctgcaccc
     9481 ggatccgcgc ctggccctgc gggacgacgg ggtaggagaa gccgatgacg tagacgccgc
     9541 gctccaggag cagctccgcc atcttggccg cctcggccgc gtcgccgatc atgacggggg
     9601 cgatggcgtg gtcgccgggg aggatgtcga agccctcctc ggtcatccgg gagcggaaca
     9661 gcgcggtgtt ggcgtgtagc cgttcacgca gctcaccggc cgactccagc aggtcgagca
     9721 ccttgagcga ggcggccgcg atcaccgggg cgagcgagtt ggagaagagg taggggcgcg
     9781 agcgctggcg cagcagcgcg acgatctcgg cgcgggccgc gacatagccg ccggaggcgc
     9841 cgccgagggc cttgccgagg gtgccggtga tgatgtcgac gcggtccatc acgtcgtgca
     9901 gttcgggggt gccgcggccg ccggggccga cgaagccgac cgcgtgcgag tcgtcgacca
     9961 tcaccatggc gccgtagcgg tcggccaggt cgcagatctc gcgcagcggt gccacatagc
    10021 cgtccatgga gaagacgccg tcggtgacga cgagcttgcg gcccgcgctc cccgcctcct
    10081 tcaactgccg ttccagatcc gccatgtcgc ggttggcgta gcggaagcgg cgggccttgg
    10141 agagccggat gccgtcgatg atggaggcgt ggttgagggc gtcggagatg accgcgtcgt
    10201 ccgggccgag cagcgt
//
