LOCUS       NZ_CP084681            10459 bp    DNA     linear   CON 30-APR-2024
DEFINITION  Streptomyces inhibens strain NRRL30439 chromosome, complete genome.
ACCESSION   NZ_CP084681
VERSION     NZ_CP084681.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces inhibens
  ORGANISM  Streptomyces inhibens
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 9696490)
  AUTHORS   Barry,C.P., Gillane,R., Talbo,G.H., Plan,M., Palfreyman,R.,
            Haber-Stuk,A.K., Power,J., Nielsen,L.K. and Marcellin,E.
  TITLE     Multi-omic characterisation of Streptomyces hygroscopicus NRRL
            30439: detailed assessment of its secondary metabolic potential
  JOURNAL   Mol Omics 18 (3), 226-236 (2022)
   PUBMED   34989730
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9696490)
  AUTHORS   Barry,C.P., Palfreyman,R. and Marcellin,E.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-OCT-2021) EMS, Australian Institute for Bioengineering
            and Nanotechnology, Cnr College Rd & Cooper Rd, Brisbane, Queensland
            4103, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. APRIL-2018
            Assembly Name                     :: AIBN_shyg
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 239.0x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022221585.1-RS_2024_04_30
            Annotation Date                   :: 04/30/2024 04:58:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,775
            CDSs (total)                      :: 8,682
            Genes (coding)                    :: 8,305
            CDSs (with protein)               :: 8,305
            Genes (RNA)                       :: 93
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 158 of 377
            Pseudo Genes (incomplete)         :: 310 of 377
            Pseudo Genes (internal stop)      :: 38 of 377
            Pseudo Genes (multiple problems)  :: 116 of 377
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 23:32:03
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 84445
            Orig. end                         :: 94904
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP084681.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Australian Institute for
            Bioengineering and Nanotechnology, Building 75,Cnr College Rd &
            Cooper Rd, Brisbane City QLD 4072.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10459
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="melanin"
                     /region_number="1"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..10459
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10459
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5459](+)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5459
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     gene            complement(819..1256)
                     /locus_tag="KI385_RS00410"
                     /old_locus_tag="KI385_00410"
     CDS             complement(819..1256)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1120: AsnC family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011027181.1"
                     /locus_tag="KI385_RS00410"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KI385_00410"
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="WP_238577504.1"
                     /transl_table=11
                     /translation="MDDVDRALIAELQRDATQAYAVLGKAVGLSAGAAHDRVRKLRERG
                     VIRRTTVEADPAALGRGVLAFVMVDSSAWMGDSAESFAAIPEIQEAHIIAGAASVLVKV
                     RTATTEQLQEVLRRLYAIEGVNGTQATVALETFFERPVCAG"
     gene            1354..2271
                     /locus_tag="KI385_RS00415"
                     /old_locus_tag="KI385_00415"
     CDS             1354..2271
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016436321.1"
                     /locus_tag="KI385_RS00415"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KI385_00415"
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="WP_238570245.1"
                     /transl_table=11
                     /translation="MSGEILNNLYEILDERFRTGRCTNGDARLERLHADCRWAEGPLYL
                     PAWRQLIWSDIPNDRLLRWDETTGAIGVFRSPAGHVNGNTLDRESRLVSCEQGNRRVTR
                     TEHDGRVTVLADRFEGKRLNSPNDAVVRSDGSVWFTDPDFGITSDYEGHRAESEIGTCD
                     VYRIDPVTGEVRRVADGFGGPNGLAFSPDERQLYVADTRARHIRVFDVHEDRTLSDGKV
                     FAERTDGGFDNIRFDNEGRLWAAAFDDGVHCYHPDGTLIGRLHVPEPVSNIAFGGPKNN
                     RLFITATTSLYSLMMAVTGLPRVR"
     gene            2411..3001
                     /locus_tag="KI385_RS00420"
                     /old_locus_tag="KI385_00420"
     CDS             2411..3001
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF039509.3"
                     /locus_tag="KI385_RS00420"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KI385_00420"
                     /product="lipoprotein"
                     /protein_id="WP_238570246.1"
                     /transl_table=11
                     /translation="MRRQIRGALGAALLAGAVFGCAGGDSGAGGDGPAVASIGGAGSAC
                     ALPVTFDVAKPWKTKRTEPVNDSSSWFHFSHACEIEAGTGDAKVTLSVHTPDGPVGDSP
                     QKVMDSFQGAGLANYGEHTSDYHYNKSKVASSPALEMDYRMSPPDLQIKPSRHRRLAVV
                     TPKGTVVLSADGSEGPASRAFQLAKSSMKVTSS"
     misc_feature    2858..2860
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            3453..3986
                     /locus_tag="KI385_RS00425"
                     /old_locus_tag="KI385_00425"
     CDS             3453..3986
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1111: GCN5-related N-acetyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003978644.1"
                     /locus_tag="KI385_RS00425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KI385_00425"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_238570247.1"
                     /transl_table=11
                     /translation="MTEIRTPRLLLRRWHDDDLTPMADINADPRVMHWIDDGSVLDLDH
                     TAEDIERWEEEWDEEGFGLFAVELLASGELAGFTGLSVPEFLPEVGPAVAISWRLGSQF
                     WGQGYASEAAQATLEFALQDRGLDRVISISRVGDDASENVIRKLGMVLERETEHPVYGF
                     PLRVHAIDLTEFQA"
     misc_feature    4371..4384
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 19.41"
                     /tool="antismash"
     misc_feature    4764..4781
                     /note="TFBS match to HipB, Bacterial antitoxin HipB,
                     confidence: weak, score: 20.64"
                     /tool="antismash"
     gene            4795..4938
                     /locus_tag="KI385_RS00430"
                     /old_locus_tag="KI385_00430"
     CDS             4795..4938
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="KI385_RS00430"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="KI385_00430"
                     /product="hypothetical protein"
                     /protein_id="WP_238570248.1"
                     /transl_table=11
                     /translation="MSTIAAVESCTFAQVLSRSLPDSPVQLDDPFVPASMWRRGPTVGA
                     PA"
     gene            5001..5459
                     /locus_tag="KI385_RS00435"
                     /old_locus_tag="KI385_00435"
     CDS             5001..5459
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608161.1"
                     /locus_tag="KI385_RS00435"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KI385_00435"
                     /product="tyrosinase cofactor"
                     /protein_id="WP_238570249.1"
                     /sec_met_domain="melC (E-value: 1.4e-38, bitscore: 131.2,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSRPTRRQVLHSSAAALAGAALAAPVAFANTAAARPADGHGHLAG
                     HGHSGGHEMPEPFDEMYQGRHIEGWPADEGGSENGGHGDHHAGQAGPHEGMDFVVRIDN
                     DDLHVMRNADGTWISLVNHYETFTTPRALARAAVRELQGAALVPVVTG"
     gene            5501..6319
                     /locus_tag="KI385_RS00440"
                     /old_locus_tag="KI385_00440"
     CDS             5501..6319
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018091617.1"
                     /locus_tag="KI385_RS00440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KI385_00440"
                     /product="tyrosinase family protein"
                     /protein_id="WP_238570250.1"
                     /transl_table=11
                     /translation="MAVRKNQADLTATEKRNFVDAVLELKRTGRYDEFISTHNAFIIGD
                     TDDGERTGHRSPSFLPWHRRFLLQFEQALQSITPSVMLPYWDWTADRTPRSPLWADDFL
                     GGDGRSGDGRVTSGPFAFGNGNWPMNIRVDGRTFLRRSLGTGVRELPTRAEVDRVLAMT
                     VYDAAPWNSSSDGFRNNVEGWRGPNLHNRVHVWVGGQMTTGASPNDPVFWLHHCFIDKL
                     WAEWQRMHPKSGYLPTGDTPDVVDLHDTMKPWNDVTPADLLDHTKFYNYA"
     gene            <6415..>6567
                     /locus_tag="KI385_RS00445"
                     /old_locus_tag="KI385_00445"
                     /pseudo=""
     CDS             6415..>6567
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611360.1"
                     /locus_tag="KI385_RS00445"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /old_locus_tag="KI385_00445"
                     /product="IS5/IS1182 family transposase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MDSTVCRAHQHAAGARRRAPRFAGKRTRPAGHRPDEAPGRSRGGL
                     TTKIHL"
     misc_feature    6640..6654
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: medium, score: 19.02"
                     /tool="antismash"
     misc_feature    7095..7110
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 20.65"
                     /tool="antismash"
     gene            7441..8541
                     /locus_tag="KI385_RS00450"
                     /old_locus_tag="KI385_00450"
     CDS             7441..8541
                     /EC_number="3.1.3.16"
                     /GO_function="GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1054: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006607388.1"
                     /locus_tag="KI385_RS00450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KI385_00450"
                     /product="PP2C family protein-serine/threonine phosphatase"
                     /protein_id="WP_238570251.1"
                     /transl_table=11
                     /translation="MKVLQPTLAKVPWILAALALVAGVLLDLFAPQPYTGLPLLAAAPL
                     VAGATLSFRSALAVVCVTCVVSVAVDIERGRPATPLFVDLAVVGLIGVLALAVNRLIVR
                     QGRDLALARDVAEAVQRAVLPDPPREAGPLAVAAGYTAAQAEARIGGDFFVVQETPFGV
                     RMIIGDVRGKGIAAVAAVSVAIGAFRQEAEYAPTLVTLGQRMDEALARAAARSGPVIST
                     EGFTTAVLAEVSAEGEVLSLVNRGHPPPYLVHDGQFVRLDPTAPQLPLGVGLGDIAPAV
                     SVPVDVVRLPPGASLLLVTDGVTEARDKRGTFYDPVSSGRMHRRFRDPDALVDALAKDV
                     DRWTEGEHEDDMAILVVTRRRPPRAA"
     gene            complement(8903..9793)
                     /locus_tag="KI385_RS00455"
                     /old_locus_tag="KI385_00455"
     CDS             complement(8903..9793)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003966108.1"
                     /locus_tag="KI385_RS00455"
                     /note="predicted Zn-dependent protease with a strongly
                     conserved HExxH motif; predicted Zn-dependent protease with
                     a strongly conserved HExxH motif; predicted Zn-dependent
                     protease with a strongly conserved HExxH motif; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /old_locus_tag="KI385_00455"
                     /product="DUF2268 domain-containing putative Zn-dependent
                     protease"
                     /protein_id="WP_238570252.1"
                     /transl_table=11
                     /translation="MKIVVHDTASAMLDLLQRPLEERPDALREMLSPLQSAMSIVGDMD
                     LVQMHRQGNSGFRLDREDPRYLPALRQMQDAGVWNQIEDSLATAWQRISGAVPGIKHAE
                     TVHVVLVLGDPDDDHLMVRSAGYFGMGGFPGAIHLVMWPTDTSLKKIGHCAAHELHHNV
                     RYANVVWNPMTVTVGEHVVAEGLAEAFVRELAGEQAMGPWSKALTGAGLDSAYEKITAD
                     IDVAGMQNLLAYVLGDATAQRMGQQPVGLPDFAGYGAGLRIVDAHLAASGLTAAQSTAL
                     SAREILVNAGVPTSA"
     misc_feature    complement(10130..10144)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: medium, score: 18.58"
                     /tool="antismash"
     misc_feature    complement(10137..10151)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.74"
                     /tool="antismash"
ORIGIN
        1 gcacgcgctc ctcgacgacc agggtctgct gaccgcttgg cgcaccgccg ccgcgggcgc
       61 gctgatcacc catgccatgg ctcggcccac cgccgccacg ctggccgtct tcggcaccgg
      121 cgaacaggcc cgcctccagg cgtcctggct ggccaagctc cgcccggtcg gcatggtgct
      181 ggtacacggc cgcaacccgc acaaagccaa cgccctgtgc gacgagctca acgcacgcgg
      241 cctgaacgcc cggcctgctt cggcgcaaga ggcggccgaa gccgacatga tcatcaccac
      301 gactccggca acagcccccg tcctggatgc cgcccaggta cgcgagggct cacacgtgac
      361 cggcatcggc acggacatgc cgcacaagaa cgagctgccg ccggcgctct ttcgtcgcgc
      421 acgcctcatc gcaaccgacg accacgccca gtgcctcgac cacggtgact tcggccacgc
      481 cgtccgcgcc ggggccgccg cccaggacgg cgacatcgca gccggcctgc tgctcaaagc
      541 gcccgtcgac cggccggaca ctgcgatcac ggtcgccgac ctcaccggcg tcggagcact
      601 cgacgcggca ctcgcctcgg ccgtcctcgg gcgactcctg cggtgaacac ccaggaggtg
      661 gacgggccgt gaccgtccat aacaacaagc cggaaataac ctcaggcgaa ccagccttct
      721 cgagaccgtg cccgcggccg gacaccgaag actcactgtc ccggacacac gactggccaa
      781 gccgttcacg gtagggcgca tcccggcgcc ccgcagcatc acccggcgca aaccggcctt
      841 tcgaaaaagg tctccagcgc gaccgtcgcc tgtgtcccgt tcacgccctc gatcgcgtac
      901 agccgtcgca ggacctcctg cagttgctcg gtggtggccg tgcgcacctt gacgaggacg
      961 gaggcagcgc cggcgatgat gtgggcttcc tgaatctccg ggatggcggc gaaggattcg
     1021 gccgaatccc ccatccaggc cgacgagtcg accatgacga aggcgagcac gccccgcccg
     1081 agggcggccg gatcggcctc cacggtcgta cggcggatga caccgcgttc acgcagcttg
     1141 cgtacgcggt catgagccgc gccggccgac aggccgaccg ccttgccgag caccgcgtag
     1201 gcctgtgtgg cgtcgcgctg aagctccgcg atcagcgccc tgtcgacgtc gtccacgatc
     1261 ccgttgacca tacccatatc tccaccatat ccagttcgaa cacactaaga tcgcagcgaa
     1321 tgcgattcgg ctgcaatcga gtaggggtgg accatgtccg gcgagatact caacaatctg
     1381 tacgagatcc tggacgagcg ctttcgcacc gggaggtgca ccaacggcga cgcacggctt
     1441 gagcggctgc acgccgactg ccgttgggcc gagggaccgc tctatctgcc cgcctggcgg
     1501 cagctgatct ggagcgacat ccccaacgac cgcctgttgc gctgggacga gacaacgggc
     1561 gcgatcggcg tcttccgctc ccccgccggg cacgtcaacg gcaacaccct cgaccgcgag
     1621 agccggctgg tgagctgcga gcagggcaac cggcgggtca cccgcaccga gcacgacggc
     1681 cgcgtcaccg tgctcgccga ccgcttcgag ggcaagcgcc tcaacagccc gaacgacgca
     1741 gtcgtacgct ccgacggctc ggtctggttc acggaccccg acttcgggat caccagcgac
     1801 tacgagggac accgcgccga gagcgagatc ggcacctgcg acgtctatcg gatcgacccg
     1861 gtgacaggcg aggtccgccg ggtcgcggac ggtttcggcg gaccgaacgg cctggccttc
     1921 tcgccggacg aaaggcagtt gtacgtcgcc gacacgcgcg cccgccacat ccgcgtcttc
     1981 gacgtccacg aggatcgcac cctctcggac ggcaaggtct tcgccgagcg caccgacggc
     2041 ggcttcgaca acatccgctt cgacaacgaa ggacgactgt gggccgccgc gttcgacgac
     2101 ggcgtgcact gctaccaccc cgacggcacc ctcatcggca ggctgcacgt gcccgagccg
     2161 gtctccaaca tcgccttcgg cggcccgaag aacaaccggc tgttcatcac ggcaacgacc
     2221 tcgctgtact cactcatgat ggctgtgaca ggcctgcccc gggtgcggtg accgctgtac
     2281 gaccgtgacg ccgccaccct cccggccagc gcatcgtgcc cagcgcacca ctggccaact
     2341 cagccactca gcgcccccgc gctgagacgc ggcacccacg ccacgtaccg gcccgtcgcg
     2401 cgggttccgg atgcgaaggc agatacgagg ggcgctgggt gccgcgctgc tggcgggggc
     2461 agtcttcggt tgtgcgggcg gggacagcgg cgcgggcggg gacggccccg cagtcgcgtc
     2521 gatcgggggt gccggttcgg cgtgtgccct gccggtgacg ttcgacgtgg cgaagccctg
     2581 gaagacgaag cggaccgagc cggtcaacga cagcagctcg tggttccact tctcacacgc
     2641 atgcgagatc gaggccggga caggggacgc gaaggtgact ctctccgtcc acacccccga
     2701 cgggcctgtc ggcgacagcc ctcagaaggt gatggattcc ttccagggcg caggcctggc
     2761 caactacggt gagcacacga gcgactatca ctacaacaag tcaaaggtcg cttcgagtcc
     2821 tgccctggag atggactatc ggatgagccc gccggattta cagatcaagc cctcacggca
     2881 ccggcgtctc gcagtggtga cgcccaaggg aacggtcgtc ctgtcggcgg acggaagcga
     2941 gggccccgca tcgcgggcgt tccagctcgc gaagtcctcc atgaaggtca catcctcctg
     3001 atgctccgtc agatacggcg gatctccccg gggtcccagg acggcaacac cgccaaaggc
     3061 gaggatcaac aggaccccag gaagagagca aacgcagccg ccggcagata gctgactcgc
     3121 cgcttccaaa cggcgtggag ctcttcagtg aaggaacgac gcagcagcag ccaactgctg
     3181 cggagtgcac agacgatcac tcccagactg gctgaaaatc ccaggccgcg cacctccggc
     3241 cggccttcgc acagcttgag atccacgccg cccgggcctg gcagatgcgg gccctgtcct
     3301 tcgggtatga gctgcgcagc gatccacgca gcactcgatg ttcccccgtc acggtgtggg
     3361 ctgcaggcgg cctgatgtct tgggggcctg gaacggcagc acggcaggtg ccgattaccg
     3421 ctgaggctct tgccttgggc aggatgggcg ccatgaccga gattcgcacc ccccgcctcc
     3481 tgctccgtcg ctggcatgac gacgacctca cgcctatggc ggacatcaac gctgacccgc
     3541 gggtcatgca ctggatcgac gacggttcgg tgctcgacct ggaccacacg gcggaggaca
     3601 tcgagcggtg ggaggaggag tgggacgagg agggcttcgg tctcttcgcc gtcgagctgc
     3661 tggcctcggg ggaactggcc ggtttcacgg gtctgtccgt gcccgagttc ctgcccgagg
     3721 tagggcccgc cgtggcgatc agctggcggc tcggctcaca gttctggggc cagggatacg
     3781 cgtccgaagc cgctcaagcc acgctggagt tcgcgctcca ggaccgtggc ctcgaccgcg
     3841 tcatcagcat cagccgggtg ggtgacgatg cctccgagaa cgtcatccgc aagctcggca
     3901 tggtgctgga acgcgagacg gaacacccgg tgtacggttt tccgctgcgc gttcacgcca
     3961 tcgatctcac cgagttccag gcgtgagccg accgatccgt ccgatcatca cgagacagcc
     4021 gaccggtccc catacgtcct cgatacgtcc tcgacgacag tgaccgccgc gcggtgggac
     4081 acccagagga cgccgaaacc caccgcggag tccggcaacg caaagagcgg tcgcgcagcc
     4141 gggcggcgtt acgggcctca cgcgcggcgc gggccagccg ctcggcttgc tcggtctcgg
     4201 acatcaccgg gaccagccat agttccttga ccgcgacgtc gatgcgcagg gcctcgtcgc
     4261 gggccgtcca gacccgtccg aatccccctg agccaagttc ggagaccagc cgatagcggc
     4321 cgccgatcac ctggcccggt cgcgtgctct gtgccacggc cacccccgcc ttgatccaga
     4381 tccggtccag gaagggcaca ccatgggtca tggtgagagt gaccaggcct caccggactg
     4441 ccctgcaagg gccagtggcc gtggcgcccc aggtcttggc gtccacgatg cggcggtggt
     4501 tgttgcgcag gtcgcggtgt caccgtcgtt gtcccagacg taggcgcggc ggtcctgctc
     4561 gcaagttccc gttccgagcc ggcagggctc aacacgtgag gattcaaggg ccagttcact
     4621 gccacgtcgt agcagcgcgc gggcctcaaa ccccgcgcgc ccgaagcccc ccgcattcgc
     4681 gccgagttcc ggccgtgcgg ggtctcacgg tttcgatcgc gcgagaaaag aatccgcggc
     4741 ggatggcggc caccatcgcg gaatatccgg ttccgcggct atgtgcgacg gggcatgagc
     4801 acgatcgccg ccgttgaatc ctgcaccttc gcccaggtgt tgtctcgttc gttgcctgat
     4861 tcgccggtgc aacttgatga tccgttcgtc cctgccagca tgtggcggcg cgggccgact
     4921 gtcggcgcgc cggcttgaag agccgaatct tctgcggccc tgccccggaa gaggagccga
     4981 gacgaacaga ggagtggaac atgtcgcgtc ccacccgacg ccaggtgctg cacagcagcg
     5041 ccgcggccct ggccggggcg gcgctcgccg caccggtcgc cttcgccaac accgctgccg
     5101 cccgccccgc cgacggccac ggacacttgg cggggcacgg gcactccggt ggtcacgaga
     5161 tgccggagcc cttcgacgag atgtaccagg gccgccacat cgagggctgg ccggcagacg
     5221 agggaggttc ggagaacgga ggccatgggg atcaccacgc cggccaggcc ggcccgcacg
     5281 agggcatgga cttcgtcgtc cgcatcgaca acgacgacct ccatgtgatg cggaacgcgg
     5341 acggcacctg gataagcctg gtgaaccact acgagacgtt caccacgcca cgcgccttgg
     5401 cgcgcgccgc cgttcgtgaa ctccagggcg ccgctctggt ccccgtcgta accggctgag
     5461 gcctgccccc tgaccgtccc cccaccgagg agtcctcgac atggcagtcc ggaagaacca
     5521 ggcggacctc accgccaccg aaaagcggaa cttcgtcgat gcggtgctgg agctcaagcg
     5581 caccggccgc tacgacgagt tcatcagcac ccacaacgcc ttcatcatcg gtgacaccga
     5641 cgacggagag cggaccggcc accggtcacc gtcattcctt ccctggcacc gcaggttcct
     5701 gctgcagttc gaacaggccc tgcagagcat cacgccgtcg gtcatgctgc cttactggga
     5761 ctggaccgcc gaccgcacac cccgcagccc cctgtgggcc gacgacttcc tcggcggtga
     5821 cgggcggagc ggcgacggcc gggtgacctc ggggccgttc gccttcggca acggcaactg
     5881 gccgatgaac atacgggtgg acggccggac gttcctgcgc cgctcgctcg gcaccggggt
     5941 ccgcgaactg ccgacccgag ccgaggtcga ccgggtgctc gccatgaccg tctacgacgc
     6001 cgccccctgg aacagctcgt ccgacggctt ccgcaacaac gtcgagggct ggcgcggtcc
     6061 gaatctgcac aaccgggtgc acgtctgggt cggcgggcag atgaccaccg gcgcctcgcc
     6121 caacgacccg gtcttctggc tgcaccactg cttcatcgac aagctctggg ccgaatggca
     6181 gaggatgcac cccaagtcgg ggtatctgcc gaccggcgac acacccgatg tcgtcgacct
     6241 ccacgacacg atgaagccat ggaacgacgt caccccggcg gacctgctgg accacaccaa
     6301 gttctacaat tatgcatgac aggtccctga catatgccgt gacagggacc tgatcgcggc
     6361 actgtccggg gccggaccgc ggcaccggat ccccgtccgc caccgcgggc cgccctggac
     6421 tcgaccgttt gccgtgctca ccagcatgct gccggtgcgc gaagacgtgc accgcgcttc
     6481 gcgggcaagc gaacccgccc cgcggggcat cgcccggatg aggctccggg acgctcccgg
     6541 ggcggcctga cgacgaagat ccacctggcc ggtgagggcg gccgaccccc tgtagccctg
     6601 ctggtgaccc caggtcagtg gggtgacggc ccgcagatga tcacggtcct cgatcgcatc
     6661 cgcgttcccc gttcaagcgg cacaggccca ccgccacaac cgtggcagcg caggtggtgg
     6721 gcccgccggc ttcaaccgcg agctgtaccc caccggccag cccctggtgc tgccacgccg
     6781 gcggcaacaa ccgacctcac atcgctgcgc gtatatccgg gacatgcacc cctcgctgga
     6841 cgttcagcgc ctcatggtgg gcggagcagc gatccgttcc atcccgtctg aatgcggcgt
     6901 ggtgctgcca tcgggcgcgc agggcggtga ggtcggcgca ccagctcatc accggcagct
     6961 accgcgacca gctcggcgag cggtgctctc ctgggccgtc agggcgaggc cgacgttcgt
     7021 cgtcgaatgg ccgcgctgcc ggaccgctca tcgccacgcg tatgcatcgc gtgaccgacg
     7081 gttcaccgat cgtgtcgaac ggatgtgccg gagcggctcc ctgcccacga tcacctcagg
     7141 atcgcgccgc ctcgtcccgc cctggcccgc ctcgtcccgc cctggcctgg cctggcccgg
     7201 cccggcccgg cctggccgtg aagcccgacc cggtggagtc cgatgaccgg ccgatgacgg
     7261 gaaaagtgcg ggtgaaccgc ccttggccac gggtgggagt gcgcctgcaa cgagcacgtc
     7321 tggctcacaa ggcgtatatg tgaagccatt ggaagtacta acagggtgcg gtaatccaaa
     7381 ccgtgcggtg gctccgttca ttccgggttg cgagcgtggc ccggcagcag gagatgcact
     7441 gtgaaagtcc tgcaacccac gctggcgaag gtgccgtgga tactcgcggc gctggccctc
     7501 gtggccggcg tcctgctgga ccttttcgcg ccacagccgt acacgggcct cccgctgctg
     7561 gccgccgcac cgctcgtcgc gggcgccacg ctctccttcc gctccgcgct cgccgtcgta
     7621 tgtgtcacgt gcgtggtctc ggtggcggtg gatatcgaac gcggacgtcc ggcgacgcca
     7681 ttgttcgtcg atctggccgt ggtcggcctg atcggcgtgc tggctctcgc ggtcaaccgg
     7741 ctcattgtgc ggcaagggcg cgatctggcg ctggccaggg atgtcgcaga ggcggtccag
     7801 cgggcggtgc tcccggaccc gccgagggag gccgggccgc tggccgtggc tgcggggtat
     7861 acggcggcgc aggccgaggc gcggatcggc ggtgatttct tcgtggtgca ggagacgccg
     7921 ttcggggtgc ggatgatcat tggcgatgtg cggggcaaag gtattgcggc tgtcgcagcc
     7981 gtgtcggtcg cgatcggagc gttccgtcag gaggccgagt acgctcccac cttggtcacg
     8041 ctgggacagc ggatggacga ggcgctggcc agagcagcgg cccggagcgg cccggtgatc
     8101 tccacggagg gcttcaccac cgccgtactg gccgaggtct ccgccgaagg cgaggtactg
     8161 agcctggtga accgtgggca tccgcctccg tacctggtgc acgacggaca gttcgtacgg
     8221 ctcgacccga ccgccccgca gctacccctc ggggtggggc tgggcgatat cgcccctgcg
     8281 gtctccgtgc ctgtcgatgt ggtgcggctg cccccggggg cctcgctgct actggtgact
     8341 gacggtgtca ccgaggcgcg ggacaagcgg gggactttct atgatccggt ctcctcaggg
     8401 cgcatgcaca ggcggttcag ggaccccgat gcgctggtgg atgccctcgc caaagacgtc
     8461 gaccgctgga ccgaggggga acacgaggac gacatggcca tcctggtggt caccaggcgc
     8521 cgccctcccc gcgccgcctg atgactcacc tgatgccgcc gatgaaggag ttcgtcgcag
     8581 cggaacaagg actggccgac cgtcttccag ctgccctcct acgcccccga tctcaacccg
     8641 caggaaggca tctgggccct ggtcaagcac ggcatcggca acctcgcggc cgccaacgtc
     8701 gaccacttgg ccaagaccgt ccgaaaccgc ctcaacccct gggcaccgct ctgagctggg
     8761 gttgcgccag tcttcggcgg tggctggccg agttctctgc accggtccac acctggcacg
     8821 cgcctacacg acgggccggc cttgctcgcg ttctcaacgg gccccggaca ccgcgccctt
     8881 ctgggcgcga ctgccggggg ggtcacgcgc tggtcggcac gcccgcgttg acgagtatct
     8941 cgcgcgccga cagggcggtg ctctgggcgg cggtcagacc ggaggccgcc aggtgggcgt
     9001 ccacgatccg cagtccagcc ccgtacccgg cgaagtccgg cagtcccacg ggctgctgcc
     9061 ccatgcgctg cgcggtggca tcgcccagca catacgcgag caggttctgc atcccggcca
     9121 cgtcgatgtc ggcagtgatc ttttcgtaag cgctgtccag tccggcaccg gtcagcgcct
     9181 tcgaccacgg gcccatggcc tgctcgccgg ccagctcccg cacgaacgcc tccgccagtc
     9241 cctcggcgac gacatgttct cccaccgtga cggtcatcgg gttccagacc acattcgcat
     9301 agcgcacgtt gtggtggagc tcgtgcgcgg cgcagtggcc gatcttcttc aggctcgtgt
     9361 cggtaggcca catcaccaga tggatcgcac ccgggaaccc gcccataccg aagtagccgg
     9421 cactgcggac catcagatgg tcgtcgtcgg ggtcgccgag caccaggacg acgtgcacgg
     9481 tctcggcgtg cttgatgccg ggaaccgcac cgctgatccg ctgccacgcg gtggcgagag
     9541 agtcctcgat ctggttccac acccccgcgt cctgcatctg acgcagggcg ggcaagtagc
     9601 gtgggtcctc acggtcgagt cggaagccac tgttgccctg acggtgcatc tggaccaggt
     9661 ccatatcacc cactatggac atcgcgctct ggagcgggct gagcatttct cgtagggcgt
     9721 cgggccgctc ctccaacggt cgctgcagga gatcgagcat ggcggacgcg gtgtcgtgca
     9781 caacaatctt catgactcag gaccgtaggg cctcacgtta cgggaggagc aagccgtgtt
     9841 gccgcggccg gcaattgtgg agcgaagcga ggtcactggc cgtccggcag atgcccgcag
     9901 ccaactgagc cgatcttggt gacatctagg gccgatgtgc ggccccaccc cgcgccgggg
     9961 ccgatcaccg atccgctcgg ttcgctcagc gcagttccag gccgagttcc ttctccagca
    10021 cggtcgcctg ttcgggcgtg gcaaaaacga agtagctctc gtagttggcg tccctgagat
    10081 gcacgaactg gaccaggtga aatcggcgcg tgtcgtcgcc ggggtcggga tcgacatggc
    10141 cgatgaattc catgatcgcc aggtggtcga ggtactcatc cgactggtgc tcggtctgtt
    10201 gggtcaccaa gacgccgttg cgggcgaact ccaggacctc gccgtactcg ccaccgtgca
    10261 gcctgacgtc ggtgatggtg accgcgccgt cggtgagtgc cgcggcctcc tccagaatgc
    10321 ccctgtagct ctcctccaga tcgtcgacat catccccgtg ggccgagatc gccacaccga
    10381 acgcttcgag cgcgtcgggc acttcatggg gtggcagctc atcgtgcgcg aactcggcgt
    10441 acttcgcgag cacgctgtc
//
