LOCUS       NZ_CP035253            10297 bp    DNA     circular CON 19-DEC-2024
DEFINITION  Streptococcus pneumoniae strain TVO_1901933 chromosome.
ACCESSION   NZ_CP035253
VERSION     NZ_CP035253.1
KEYWORDS    RefSeq.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2130843)
  AUTHORS   Rosconi,F., Cotton,S.L., Kempchinsky,A., Surujon,D., Johnston,C.D.
            and van Opijnen,T.
  TITLE     Streptococcus pneumoniae DNA-modification patterns determined at the
            pan-genome level
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2130843)
  AUTHORS   Rosconi,F., Cotton,S.L., Kempchinsky,A., Surujon,D., Johnston,C.D.
            and van Opijnen,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2019) Biology, Boston College, 140 Commonwealth
            Avenue, Boston, MA 02467, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 05-NOV-2018
            Assembly Method                   :: HGAP v. 4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 299x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022070145.1-RS_2024_12_19
            Annotation Date                   :: 12/19/2024 10:37:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,215
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 74
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 69 of 149
            Pseudo Genes (incomplete)         :: 70 of 149
            Pseudo Genes (internal stop)      :: 56 of 149
            Pseudo Genes (multiple problems)  :: 39 of 149
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 12:26:59
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1923453
            Orig. end                         :: 1933750
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP035253.1.
            Genome not circularized.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10297
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="7"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10297
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10297
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5297](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5297)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(842..1714)
                     /gene="rsmA"
                     /locus_tag="EQH27_RS09905"
                     /old_locus_tag="EQH27_10440"
     CDS             complement(842..1714)
                     /EC_number="2.1.1.182"
                     /GO_function="GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /gene="rsmA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519622.1"
                     /locus_tag="EQH27_RS09905"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EQH27_10440"
                     /product="16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="WP_001216882.1"
                     /transl_table=11
                     /translation="MRIADYSVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEID
                     DQVNVIEIGPGIGALTEFLTERAAQVMAFEIDHRLVPILADTLRDFDNVTVVNEDILKV
                     DLAQHIQNFKNPDLPIKVVANLPYYITTPILMHLIESGIPFCEFVVMMQKEVADRISAQ
                     PNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRPEPAVAVEDENFF
                     FKVSKASFTHRRKTLWNNLTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLAEFAGL
                     ADALKGQGL"
     gene            complement(1965..2258)
                     /locus_tag="EQH27_RS09910"
                     /pseudo=""
     CDS             complement(1965..2258)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333255.1"
                     /locus_tag="EQH27_RS09910"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MCEKIRIRRVSDYPSARGGLEDILIMENMTNHLLLVQILVHGYLL
                     DFASIEG"
     gene            complement(2251..2892)
                     /locus_tag="EQH27_RS09915"
                     /old_locus_tag="EQH27_10445"
     CDS             complement(2251..2892)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565534.1"
                     /locus_tag="EQH27_RS09915"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EQH27_10445"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_000565531.1"
                     /transl_table=11
                     /translation="MIDIQGLEKKFNDRAIFSGLNLKLEKGKVYALIGKSGSGKTTLLN
                     ILGKLEKIDGGRVLYQGKDLKTIPTREYFRDQMGYLFQNFGLLENQSIKENLDLGFVGQ
                     KISKVERLERQVGALEKVNLGYLDLEQKIYTLSGGEAQRVALAKTILKNPPLILADEPT
                     AALDPENSEEVMNLLVDLKDENRIIIIATHNPLVWNKADEIIDMRKLAHV"
     gene            complement(2894..4924)
                     /locus_tag="EQH27_RS09920"
                     /old_locus_tag="EQH27_10450"
     CDS             complement(2894..4924)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825281.1"
                     /locus_tag="EQH27_RS09920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EQH27_10450"
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="WP_000825259.1"
                     /transl_table=11
                     /translation="MKRLFILISMVLVSLYMVITSVDHREEILFGNYPSVDVTGMMINQ
                     PVASREEVTEALSHLAVEHNSLIARRIVEPNEAGETRFTYATYGEGKLPEGLTISSKES
                     AETSDLLGSYLIVSGSLDGVSLQTTLKELGYQGFVSNGEDPFSIVLLLTATPMVLLSLA
                     IFLLTFMSLTLIYRIKSLRQAGIRLIAGESLFGVALRPVLEDVRQLICSVLVSSLLGLG
                     ILWYQGALFMATVQLVIIALLLYGLTLAGISTLLSVVYLLGLQENSLVDLLKGKLPLKR
                     MMTLMMVGQLLAVLVVGSSATALLPHYREMQEMERASNKWSQSSDRYRLSFGWSSAFAD
                     EEGTRKDNREWQTFTEERLANTDSFYIMSNVDNFSDGAEVDLDGNRLSDYTPSGNVIYV
                     SPRYLIEEKITVSSEFMDKMQNLSEGEFGLILPESLREQSIYYQGLFTDYLQNFSSESV
                     EVTSQKHYLPQVRLAFTEIGQERFLYNDGYKTTRQYLKDPIIVVLTPQATGTRPVAGML
                     WGTTANSALKLDRYGDSITALKEQGLYHKVSYLVKSQLFFAKVLNDKRVEFYSLLIGTI
                     LTLSTAILLFDSMNLLYFEQFRRDLMIKRLAGMTIYELHGKYLLAQGGVLLLGLVLSSI
                     LTRDGLISALVVALFTLNALLILVRQDKKEEAGSMAVLKGK"
     gene            complement(5001..5297)
                     /locus_tag="EQH27_RS09925"
                     /old_locus_tag="EQH27_10455"
     CDS             complement(5001..5297)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Lactococcin_972"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815395.1"
                     /locus_tag="EQH27_RS09925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EQH27_10455"
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_000815392.1"
                     /sec_met_domain="Lactococcin_972 (E-value: 6.2e-20,
                     bitscore: 68.6, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKQTVKKLALVASIAATLGGGVSVASAAVQYPEGGVWTYGSGNGG
                     AYSNYYHPSKYHSSTVVSRKTGSSDKGYAGAGGTSRAWIRTSWGEKVAFYYNV"
     misc_feature    complement(5447..5460)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 22.05"
                     /tool="antismash"
     misc_feature    complement(5537..5546)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: strong, score: 18.31"
                     /tool="antismash"
     gene            complement(5617..6516)
                     /locus_tag="EQH27_RS09930"
                     /old_locus_tag="EQH27_10460"
     CDS             complement(5617..6516)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904853.1"
                     /locus_tag="EQH27_RS09930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EQH27_10460"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_000904859.1"
                     /transl_table=11
                     /translation="MLIGQKIKEIRIEKGISRPDFCGDEQELTVRQLSRIESGASQPSL
                     PKLDYIARRLGVPVYSLMPDFSALPSAYLELKYQILREPIYGKEEEYDKKEACLEEIYK
                     TYFDNLPKEEQLACEVLQACLDTSRTRRPEYAELILEEHMPQIIEKEAYSINDMLLIRL
                     FFYQMLIRKDLAKFINQIEKLMLFLLEQKKVTQIENYFIIRDTLISGMCCLEKVGVTDC
                     FNDYLSCLQEIMDKTQDYQKKPLVFMFLWKQALRVERDFSLAESFYQSSKTFAKLIGDG
                     FLVKKLTEEWQEDVKKYL"
     gene            complement(6588..7148)
                     /gene="rnmV"
                     /locus_tag="EQH27_RS09935"
                     /old_locus_tag="EQH27_10465"
     CDS             complement(6588..7148)
                     /EC_number="3.1.26.8"
                     /GO_function="GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA]"
                     /GO_process="GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /gene="rnmV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660004.1"
                     /locus_tag="EQH27_RS09935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EQH27_10465"
                     /product="ribonuclease M5"
                     /protein_id="WP_000660005.1"
                     /transl_table=11
                     /translation="MKEKISQVIVVEGRDDTVNLKRYFDVETYETRGSAINAQDIERIQ
                     RLHQRHGVIVFTDPDFNGERIRRMIMTAIPTVRHAFLKRDEAVPKSKTKGRSLGIEHAS
                     YEDLKTALAQVTEQFEHESQFDISRSDLIRLGFLAGADSRKRREYLGETLRIGYSNGKQ
                     LLKRLELFGVTLAEVEEAMKSYE"
     gene            complement(7148..7921)
                     /locus_tag="EQH27_RS09940"
                     /old_locus_tag="EQH27_10470"
     CDS             complement(7148..7921)
                     /EC_number="3.1.-.-"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575597.1"
                     /locus_tag="EQH27_RS09940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EQH27_10470"
                     /product="TatD family hydrolase"
                     /protein_id="WP_000575617.1"
                     /transl_table=11
                     /translation="MIFDTHTHLNVEEFAGREAEEIALAAEMGVTQMNIVGFDKPTIER
                     ALELVDEYDQLYATIGWHPTEAGTYTEEVEAYLLDKLKHPKVVALGEIGLDYHWMTAPK
                     EVQEQVFRRQIQLSKDLDLPFVVHTRDALEDTYEIIKSEGVGPRGGIMHSFSGTLEWAE
                     KFVDLGMTISFSGVVTFKKATDLQEAAKELPLDKILVETDAPYLAPVPKRGRENKTAYT
                     RYVVDFIADLRGMTTEELAVATTANAERIFGLDSK"
     gene            complement(8226..>9171)
                     /locus_tag="EQH27_RS09945"
                     /old_locus_tag="EQH27_10480"
                     /pseudo=""
     CDS             complement(8226..>9171)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_367670414.1"
                     /locus_tag="EQH27_RS09945"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /old_locus_tag="EQH27_10480"
                     /product="DUF1542 domain-containing protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="KVEVAGDGTATIVFKDGSAITIPGNQLVAQDPKAQDSTKPTAEKS
                     TVKAPAQRVDVKDITHLTDEEKVKVAILQANGSALDGATINVAGDGTATITFPDGSVVT
                     ILGKDTVQQSAKGESVTQEATPEYKLENTPGGDKGGNTGSSDANANAGGWISSHRFTKL
                     SSITSF"
     gene            complement(9377..9511)
                     /gene="rpmH"
                     /locus_tag="EQH27_RS09950"
                     /old_locus_tag="EQH27_10485"
     CDS             complement(9377..9511)
                     /GO_component="GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA]"
                     /GO_component="GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA]"
                     /GO_function="GO:0003735 - structural constituent of
                     ribosome [Evidence IEA]"
                     /GO_process="GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /gene="rpmH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /locus_tag="EQH27_RS09950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EQH27_10485"
                     /product="50S ribosomal protein L34"
                     /protein_id="WP_000831905.1"
                     /transl_table=11
                     /translation="MKRTYQPSKLRRARKHGFRNRMSTKNGRRVLAARRRKGRKVLAA"
ORIGIN
        1 tctacgattt tcaatttcac taaggaattg caggtaattg tcaaaacgga aggttgcaat
       61 aacaccctct tcaacagctg gtttgacggc acaagacggc tcatgggtat gggtacaagt
      121 acggaactta caatctcggc taacagtagc aatctctggg aaagcctgat tgaggtcttc
      181 agcccttgat acttcatagt ccaaggatga aaatcctggt gtatctgcga ttttaccccc
      241 gttgagattg taaaaactaa cagctcgagt ggtatggcga ccgcgaccta aactgtctga
      301 aatttctccc gtttcaagat tgaggtctgg tgcgatttta ttgagaagag ttgacttccc
      361 aacacctgtc tgccccataa agaccgtaac cttgcctgtt aacaaaggca ggagttcctc
      421 tttactggtc acaaagtcat agccgatgtc accataggtc tgctggtaaa aatccagttc
      481 tcccccatct tccaacaaat ccattttgga aatatagaca atgggatgga tgcccttgtg
      541 ctccaaaaga accaagaaac gatccagcaa attgctgtta aaatcaggtt ccttgacgga
      601 catgattact acagcttgat cgatattgac aataggcgga cgaaccagac tgtttttccg
      661 ttcgtgaatt tttaggatat agccttctga attttcctcg gcagagaaat ctacccagtc
      721 cccaacataa ggggtatggc cttttttacg gaaattccca cgcgcgcgtg tttgataaac
      781 ctggccatca ctctccacat agtagaaacc tgccaaggct ttaatgattt gtccctgcat
      841 cttagagtcc ttgcccttta agtgcgtctg ctagaccggc aaattctgcc aagctgagag
      901 cttccccacg cacacttggt gacaagcctg cctggtccaa agccttggtc agcttgtcct
      961 tgacctcttc agtcttacca aagtaacctg tcaagttatt ccacaaggtc ttgcggcgat
     1021 gggtaaaact agccttggaa accttaaaga aaaagttctc atcttctact gctacggctg
     1081 gctctggacg acgcaccatt ttcaagatgg ctgaatccac atttggcgct ggcacaaaga
     1141 ccgtacgagg cacgataaag gcaaccttgg ctgtcatgta atactgcacg gcgatagaca
     1201 agctaccgta agccttggtg ttaggctggg ctgaaatgcg gtccgctact tctttctgca
     1261 tcatgaccac aaactcacaa aaaggaatgc cactctcaat caagtgcatg agaataggcg
     1321 tcgtgatgta gtaaggcaaa ttagccacta ccttgattgg caggtcagga tttttaaaat
     1381 tctggatatg ttgcgccaaa tcaaccttga gaatatcttc gttaactacg gtcacattat
     1441 caaaatcacg cagagtatct gccaaaattg gcaccaaacg gtggtcaatc tcaaaagcca
     1501 tgacttgggc tgcacgctca gtcaaaaatt ctgtcaaggc accaatacct ggcccgattt
     1561 cgatgacatt gacctggtca tcaatttcag ccgtatccac aattttttga aggatattgg
     1621 tatccgtcag gaaattttgc ccgaaggact ttttaaaggt aaaaccgtga cgctccagca
     1681 ctgccttggt cacgctataa tctgcaattc tcatctattc tcttttctat tatctgttac
     1741 tactattgta acacattccc cttgtatttt gggaatgaaa atcataccaa aaaactatct
     1801 ttcctcatga agaaagatag tcttggaaat ctttaaagag ggacttagcg cttctctcga
     1861 aacatacgtt catactcttc gaaaatctct tcaaaccacg tcagcttcac cttgccgtac
     1921 tcaagtacag cctgcggcta gcttcctagt ttgctctttg attttcattg agtatcaaaa
     1981 aaatcagctt cttgataact tctattttcg ggtagggtca aatccacatc ctcctccaca
     2041 tcatccactg tcagttcaac cgtttgaggt aaatttttca aacgataatg cttttgcctt
     2101 tacccttcaa tactagcaaa atcaagcaaa tagccatgca ctaggatttg aaccaaaagg
     2161 agatgattgg tcatattttc catgatgagg atatcttcta aaccacctct ggcactagga
     2221 taatcagata ccctgcgaat acggattttt tcacacatga gcaagtttcc tcatatcaat
     2281 gatttcatca gccttattcc agactagggg attatgggtc gcaatgatga taattcgatt
     2341 ttcatctttc aaatccacca agagattcat aacctcctct gaattttcag gatcaagagc
     2401 tgctgttggt tcatctgcca aaatcaaggg tggatttttc aaaatagtct tagcaagggc
     2461 aactcgttgg gcctctcccc cagataaagt atagattttt tgttctaaat ccaaataccc
     2521 tagattaact ttttctaaag cccccacttg cctttccaaa cgttctactt ttgagatttt
     2581 ctgaccaaca aaacccaaat ccaaattttc tttgattgat tggttttcta agaggccaaa
     2641 attttgaaag agatagccca tctgatctcg aaaatactca cgagtgggaa tggtttttaa
     2701 atctttcccc tgatagagaa cccttcctcc atctatcttt tctagctttc ccaagatatt
     2761 cagcagcgtc gtctttccgc ttccactctt tccgattaag gcataaacct tgcccttctc
     2821 cagcttgaga ttcaaaccag agaaaatcgc gcggtcatta aatttctttt ccaatccttg
     2881 aatatcaatc atcttatttt cctttcaata ctgccatgct accagcttct tcttttttgt
     2941 cctgccttac taaaatcaag agggcgttaa gcgtaaacaa agctacaact agagcgctaa
     3001 tcaaaccatc tcttgtcaaa atactagata ggactaggcc aagcaagaga actcctcctt
     3061 gcgccagtaa atacttgcca tgaagctcat agattgtcat accagcaaga cgtttaatca
     3121 taagatcccg tctgaactgc tcaaaataga gaagattcat ggaatcaaat aacaagatag
     3181 ccgtagacag ggtcaaaatc gtcccaataa ggagagagta aaactccacc cgtttgtcat
     3241 ttagtacctt ggcaaaaaat agctggcttt ttaccaagta agaaaccttg tgatacagac
     3301 cttgctcttt tagagctgtg atgctgtctc catatcgatc tagtttcaag gcactattag
     3361 ccgtagttcc ccacaacatc cctgcaacag gtcttgttcc agtcgcttgc ggcgttagaa
     3421 ctacaataat cggatctttt aggtactggc gtgttgtctt gtacccatca ttatagagga
     3481 aacgttcctg tcctatttct gtaaaagcta gccttacctg tgggaggtag tgtttctgac
     3541 tcgtcacttc tacactttca gatgaaaagt tttgcaggta atctgtaaac aatccttggt
     3601 agtagataga ctgctctcgc aagctctcag gcaagatcag cccaaactct ccctcagaca
     3661 agttttgcat cttgtccata aactctgaag aaacggtaat cttttcttct atcagatagc
     3721 gcggtgagac atagataaca ttccctgacg gtgtgtagtc actgagacga ttgccatcta
     3781 ggtccacttc tgctccatct gagaaattgt caacattgct cataatataa aaagagtctg
     3841 tattggctaa ccgttcttca gtaaatgtct gccactcacg attatcctta cgcgttcctt
     3901 cttcatcggc aaatgcacta gaccaaccaa aggatagacg gtaacggtct gaggactggc
     3961 tccatttatt gctagctctc tccatttcct gcatttcacg gtagtggggt aggagagctg
     4021 tcgcactcga tccgaccacc aatacagcta agagttgccc caccatcatc aatgtcatca
     4081 tacgtttgag agggagtttc cctttcaata gatccaccag actattttcc tgtaaaccaa
     4141 gtagatagac gacacttagt aaggtagaaa tccctgccaa ggtcaatcca taaagtagaa
     4201 gagcaatgat gaccagttgc accgttgcca taaacaaggc accttgatac cagagaatcc
     4261 ccaatcccaa aagactggat accagcactg agcagataag ctgtctcaca tcttctaaca
     4321 ctggtctgag agcaactcca aacaagctct caccagctat taagcgaatc cctgcctgac
     4381 gaagggattt gatccgataa atcagggtca gactcataaa ggtcagcaga aaaatagcta
     4441 aactcagtag caccataggg gtggccgtca atagtaagac tatcgaaaat ggatcttctc
     4501 cattcgaaac aaagccttga taaccaagct ctttcaaggt ggtctgtaag ctcactccat
     4561 ccaaacttcc tgatacaatc aagtaagacc ctaataaatc actcgtttct gcactctcct
     4621 tggaggaaat ggtcagacct tctggaagct ttccctcacc ataagtggca taggtaaagc
     4681 gtgtttctcc agcttcattt ggctcaacga ttcgacgagc aatgagacta ttgtgctcta
     4741 ccgccaagtg actcaaagcc tctgtcacct cttcgcgact agctactggt tgatttatca
     4801 tcattcctgt cacatcaaca gaaggatagt taccaaataa aatctcttct cgatggtcaa
     4861 cggaagttat caccatataa agcgatacca ataccattga aatcaaaata aataaacgtt
     4921 tcatcatttc ctccaaaaag ttacttggtc acttagtgag aaggtgacca agtaaagcta
     4981 aacaataggg ctataccatt ctaaacatta tagtagaatg caactttctc tccccaagaa
     5041 gtacgaatcc atgcacgaga agtccctcca gcaccagcat atcccttgtc agatgaacca
     5101 gtttttctgc taacaactgt tgaactatgg tattttgaag ggtgatagta gtttgagtaa
     5161 gcaccaccgt tacctgaacc ataagtccaa acaccacctt ctgggtattg aacggctgca
     5221 gaggcaactg atacacctcc acctaatgtt gctgcaatgc tcgctacaag agctaacttt
     5281 ttaactgttt gtttcatatt gatactcctc tatttttgat tacaagattg ttgagttact
     5341 tgctcctctt tccattatca acgttgatac tattatatta ccaaatgata ttctaaaatt
     5401 atttgttttt ctctaaattg tcaaaatagc accctgaaat gaacttttat agtcattata
     5461 ctccatcttt atcacactat caaggacatc aatgtcctta aaatctaact atttcatatt
     5521 atttttagct tatatctaaa aaaacagttc taaagacgaa ctttagaact gttttgatac
     5581 gaggacaagg acatttttgt cactgattca ctatgtttat aaatattttt tgacatcctc
     5641 ttgccattcc tctgtcaatt tctttactag aaatccatct ccaattagct tcgcaaatgt
     5701 tttagaagac tgataaaatg attcagctaa actaaaatct ctttctactc ttaatgcttg
     5761 cttccacaaa aacataaata caagaggttt cttttgataa tcttgagttt tatccataat
     5821 ttcttgtaaa cacgatagat aatcattaaa acaatcagtt actcctacct tttcaagaca
     5881 acacattcct gaaataagag tatctctaat tataaagtaa ttctctattt gagttacctt
     5941 cttctgttcc aaaagaaaga gcattagctt ttcgatttga tttataaatt tggcaagatc
     6001 ttttctaatg agcatttgat aaaaaaacaa acgaatcaac aacatatcat ttattgaata
     6061 agcttctttt tctataatct gaggcatatg ttcctcaagt attaactctg catattcagg
     6121 ccttctagtt ctagaagtat ccaaacacgc ctgcaatact tcacatgcta attgttcttc
     6181 tttaggaaga ttatcaaagt atgttttata aatctcttcc aaacacgctt ccttcttatc
     6241 gtactcctct tctttaccat agattggttc acgtaaaatc tggtatttca attctaaata
     6301 agcagaagga agagctgaaa aatccggcat aaggctataa actggaactc ctagccggcg
     6361 agcaatatag tctaacttgg gcaaactcgg ttgcgaagct ccactttcaa ttcgcgacag
     6421 ttgacgaact gtcagttctt gctcatctcc acaaaaatct ggacgactaa ttcctttttc
     6481 tatccgaatc tctttaattt tttgaccaat taacatcaga cgcctcctct tctacctgct
     6541 tcgaaaaata ctgtgaaact taaaaaattg taacggctac atctttccta ctcataagat
     6601 ttcatagctt cttccacttc tgccaaagta accccaaaca actctaggcg tttgaggagt
     6661 tgcttgccgt tggaatagcc gattcggaga gtctctccga gatattctct acgcttacgg
     6721 ctgtctgccc ctgctagaaa accaaggcga atcaaatcgc tacgactaat gtcaaactga
     6781 ctctcatgtt caaattgttc tgtcacctga gctagagccg ttttcaggtc ttcatagctg
     6841 gcatgctcaa ttcccagaga acgccccttg gtcttggact ttggaacagc ttcatctcgc
     6901 ttgagaaagg catgccgaac tgttggaatg gccgtcataa tcatgcgccg aatccgctcc
     6961 ccattaaaat ctgggtctgt aaagacaatg actccatgac gttggtgcag gcgctgaatc
     7021 cgctctatat cctgagcatt gatggcagaa cctcgagtct cataggtctc cacatcgaaa
     7081 taacgtttga gattgaccgt atcatcacgc ccttcaacca cgataacttg agaaattttc
     7141 tctttcatta cttgctgtcc aatccaaaaa ttcgttctgc atttgcagtc gttgctaccg
     7201 ccagctcttc tgtcgtcata ccacgcaagt cagcgataaa gtcgaccaca tagcgagtat
     7261 aggctgtttt attttcacga ccacgcttgg gtacaggtgc taagtaaggc gcatctgttt
     7321 ccaccaaaat cttgtccaaa ggtaactctt tagctgcttc ttggaggtca gttgccttct
     7381 taaaagtcac cactcctgag aaggaaatgg tcataccaag atccacaaac ttctctgccc
     7441 actcaagcgt ccctgaaaat gaatgcatga taccaccacg aggaccaacg ccctcactct
     7501 tgataatctc ataggtatct tccagcgcat cacgggtatg gacaacaaaa ggcaaatcca
     7561 agtccttaga tagctgaatc tgacggcgaa aaacctgctc ctgcacctct ttgggcgctg
     7621 tcatccaatg gtaatctaag ccaatttcac ctaaagccac aaccttggga tgttttaact
     7681 tatccaacaa gtaagcctca acttcctctg tataagtacc agcttctgta ggatgccaac
     7741 caatagtcgc atagagctga tcatactcat ctaccaactc caaggcacgc tcaatcgtcg
     7801 gtttatcaaa accaacaata ttcatctgtg tcacacccat ctcagcagcc aaggcaattt
     7861 cttctgcctc acgacctgca aattcttcta cattcaagtg tgtatgtgta tcaaaaatca
     7921 tctcttctaa cctcgttttc tatcttctat tataccaaaa aagaggaggg gcacctaatt
     7981 tttcggtttc ccctcctctc ttcaatagag agctattctg ctatcttttc tatccgatat
     8041 tgcccatctc ctattccaca gttagagaca gaagagattg gctacattga tagaatattc
     8101 ctattcagct aaagtatgtg attatcttgg gttctcactt ttctcaccca tcactaggat
     8161 tgatgacttc tcaaaatccc ccttataatc taactttaag tagagaatag aattttctag
     8221 ctgttctatt ttctacgtct agtctttctt cctagcaaag caaaaccact tgttgccacc
     8281 actagaccaa gactagcaag tgctgcactt gatgctgaac ctgtgttagg taattgtttg
     8341 ccagcaggtg actggtaggt aacatcttgc attgttcctg cagttgcttg cggtgcactt
     8401 gttgtcttag gagccactgg tctcttagga actgtaacca tgacaatggc ttgcactcca
     8461 atcgtttctg cttccttcac atcttccata gttttcgcat tttcaatctc tttgagagct
     8521 gcttgttttt ctgcttccac tcttgctaaa agttctgctt tttctttatc agaaagcggt
     8581 gcgcctttga tttcatcctg cttgtccttg gctgcttttt caatggcatt tttagctgat
     8641 tctttttcag tagctaattg cttagaagct tgtgattgag ctgagttttg tgaacctgtg
     8701 tgagctgatc cacccgcctg cattcgcatt agcatctgag cttccagtat tgcctccctt
     8761 atctccacct ggtgtatttt ctagcttata ctctggtgta gcttcttgag ttacagattc
     8821 acctttcgca gattgttgaa ctgtatcttt tcctagaatc gtcactactg aaccatctgg
     8881 gaatgtgatt gttgctgtac catctccagc tacattgatt gtcgctccgt ctaatgctga
     8941 accatttgct tgtaaaatag caaccttaac tttttcttca tctgttaaat gagttatatc
     9001 ttttacatct actctttgag caggtgcttt aacagttgat ttttcagcag tcggtttagt
     9061 gctatcttgt gcttttggat cttgtgctac caattgattt cctggaattg taatagctga
     9121 accatcttta aagacgattg tcgctgtgcc atctccagct acttcaactt tatagtaaaa
     9181 tgaaataata acaggacaaa tcgatcagga cagtcaaatc gatttctaac aatgttttag
     9241 aaatagaggt gtactattct agtttcaatc tactataaca tcgcttccga atccatttgc
     9301 atctactgaa cttttttaca aaaataaaaa tcagtagact cgagttccta ctgatttttg
     9361 atagaattcg tttggattat gcagccaaaa ctttgcgtcc tttacgacga cgagctgcca
     9421 atacgcgacg accattttta gttgacatac ggttacggaa tccgtgtttg cgcgcacgac
     9481 gaagtttact tggttgataa gtacgtttca cgatgaatac ctcctcatag attttgtatt
     9541 cgtttagccg gctataaagt gatcagttac taaacatact ttactattct atctgattct
     9601 ttcacttttg tcaatagctc taggcaaatg tttccagctt tttcgaatgc aagccctatc
     9661 tacgatactg tttcaagaaa cgagtcatct tttcttggat ttgggctaac tcatcaacac
     9721 gaggaaggta aacgatacgg aagtggtctg gttcctgcca gttaaagcct cgaccatgaa
     9781 ccaagagaac cttttcctgc ttcaagaaat caaggacaaa ctgctcatca tcatcgatac
     9841 ggtacatatt gcggtcgatt tttgggaaga tatagagccc cgccttgggt ttaacggcag
     9901 acaaacctgg aatatcttga atggcattat agatgaaatt tctttgctcg tagattcgtc
     9961 caccaggaag aagcaattca tcgactgatt ggtgaccccc caaggaagtt tgtacgactt
    10021 gttgggccaa aacgttagag caaaggcgca tattggacag catattgagc ccttcgatat
    10081 agcccttaac atgagtctta gggccagaca agaccatcca acccacacgg aaacctgcta
    10141 tgcggtggga ttttgacaga ccattcatgc tgacacagaa gacatctggt gccaagctcg
    10201 ccacaggcgt atgcacatgt ccgtccatta ccatgcggtc atagatttca tccgcaaaga
    10261 taatcaaatc gttttgacgg gcaatctcaa taatctc
//
