LOCUS       NZ_CP035253            17941 bp    DNA     circular CON 19-DEC-2024
DEFINITION  Streptococcus pneumoniae strain TVO_1901933 chromosome.
ACCESSION   NZ_CP035253
VERSION     NZ_CP035253.1
KEYWORDS    RefSeq.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2130843)
  AUTHORS   Rosconi,F., Cotton,S.L., Kempchinsky,A., Surujon,D., Johnston,C.D.
            and van Opijnen,T.
  TITLE     Streptococcus pneumoniae DNA-modification patterns determined at the
            pan-genome level
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2130843)
  AUTHORS   Rosconi,F., Cotton,S.L., Kempchinsky,A., Surujon,D., Johnston,C.D.
            and van Opijnen,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2019) Biology, Boston College, 140 Commonwealth
            Avenue, Boston, MA 02467, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 05-NOV-2018
            Assembly Method                   :: HGAP v. 4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 299x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022070145.1-RS_2024_12_19
            Annotation Date                   :: 12/19/2024 10:37:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,215
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 74
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 69 of 149
            Pseudo Genes (incomplete)         :: 70 of 149
            Pseudo Genes (internal stop)      :: 56 of 149
            Pseudo Genes (multiple problems)  :: 39 of 149
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 12:26:59
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 48017
            Orig. end                         :: 65958
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP035253.1.
            Genome not circularized.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..17941
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..17941
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..17941
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:12941](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..12941
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(139..1149)
                     /locus_tag="EQH27_RS00290"
                     /old_locus_tag="EQH27_00295"
     CDS             complement(139..1149)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397796.1"
                     /locus_tag="EQH27_RS00290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EQH27_00295"
                     /product="YeiH family protein"
                     /protein_id="WP_000009171.1"
                     /transl_table=11
                     /translation="MSFLSKNGAGILVCLLISILSWYLGGFFPVIGAPVFAIFMGMLLH
                     PFLSSYKQLDVGLTFSSKKLLQYAVILLGFGLNISQVFAVGQSSLPVILSTISIALIVA
                     YLFQRFFALDTKLATLIGVGSSICGGSAIAATAPVIHAKEKEVAQAISVIFFFNVLAAL
                     IFPTLGTWLHLSNDGFALFAGTAVNDTSSVTATASAWDSLYQTNTLESATIVKLTRTLA
                     IIPITLFLSYWQSREQKNKQGLQLKKVFPLFILYFILASLLTTLLTSLGVSSSFFTPLK
                     QLSKFLIIMAMSAIGLKTNLVAMVKSSGKSIVLGAVCWIAIILTSLGMQTLIGIF"
     gene            1298..2467
                     /locus_tag="EQH27_RS00295"
                     /old_locus_tag="EQH27_00300"
     CDS             1298..2467
                     /EC_number="2.6.1.-"
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_process="GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Aminotran_1_2 (31-382). E-value:
                     8.4e-64. Score: 207.9. Matches aSDomain:
                     nrpspksdomains_EQH27_RS00295_Aminotran_1_2.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_1_2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DegT_DnrJ_EryC1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1019: aminotransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519430.1"
                     /locus_tag="EQH27_RS00295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EQH27_00300"
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="WP_000366345.1"
                     /sec_met_domain="Aminotran_1_2 (E-value: 2.6e-62, bitscore:
                     208.1, seeds: 48, tool: rule-based-clusters)"
                     /sec_met_domain="Aminotran_5 (E-value: 2.3e-07, bitscore:
                     27.0, seeds: 34, tool: rule-based-clusters)"
                     /sec_met_domain="DegT_DnrJ_EryC1 (E-value: 1.9e-07,
                     bitscore: 27.7, seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDLTKRFNKQLDKIQVSLIRQFDQAISEIPGVLRLTLGEPDFTTP
                     DHVKEAAKRAIDQNQSYYTGMSGLLTLRQAASDFVKEKYQLDYAPENEILVTIGATEAL
                     SATLTAILEEGDKVLLPAPAYPGYEPIVNLVGAEIVEIDTTENGFVLTPEMLEKAILEQ
                     GDKLKAVILNYPANPTGITYSREQLEALAAVLRKYEIFVVCDEVYSELTYTGEAHVSLG
                     TMLRDQAIIINGLSKSHAMTGWRLGLIFAPATFTAQLIKSHQYLVTAANTMAQHAAVEA
                     LTAGKNDAEPMKKEYIQRRDYIIEKMTALGFEIIKPDGAFYIFAKIPAGYNQDSFAFLK
                     DFAQKKAVAFIPGAAFGRYGEGYVRLSYAASMETIKEAMKRLEEYMREA"
     aSDomain        1391..2443
                     /aSDomain="Aminotran_1_2"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_EQH27_RS00295_Aminotran_1_2.1"
                     /evalue="8.40E-64"
                     /label="EQH27_RS00295_Aminotran_1_2.1"
                     /locus_tag="EQH27_RS00295"
                     /protein_end="382"
                     /protein_start="31"
                     /score="207.9"
                     /tool="antismash"
                     /translation="VLRLTLGEPDFTTPDHVKEAAKRAIDQNQSYYTGMSGLLTLRQAA
                     SDFVKEKYQLDYAPENEILVTIGATEALSATLTAILEEGDKVLLPAPAYPGYEPIVNLV
                     GAEIVEIDTTENGFVLTPEMLEKAILEQGDKLKAVILNYPANPTGITYSREQLEALAAV
                     LRKYEIFVVCDEVYSELTYTGEAHVSLGTMLRDQAIIINGLSKSHAMTGWRLGLIFAPA
                     TFTAQLIKSHQYLVTAANTMAQHAAVEALTAGKNDAEPMKKEYIQRRDYIIEKMTALGF
                     EIIKPDGAFYIFAKIPAGYNQDSFAFLKDFAQKKAVAFIPGAAFGRYGEGYVRLSYAAS
                     METIKEAMKRL"
     gene            2464..3234
                     /gene="recO"
                     /locus_tag="EQH27_RS00300"
                     /old_locus_tag="EQH27_00305"
     CDS             2464..3234
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006281 - DNA repair [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /gene="recO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616164.1"
                     /locus_tag="EQH27_RS00300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EQH27_00305"
                     /product="DNA repair protein RecO"
                     /protein_id="WP_000616162.1"
                     /transl_table=11
                     /translation="MIQSITSQGLVLYNRNFREDDKLVKIFTEQVGKRMFFVKHAGQSK
                     LAPVIQPLVLARFLLRINDDGLSYIEDYHEVMTFPKINSDLFVMAYATYVAALADASLQ
                     DNQQDAPLFAFLQKTLELMEAGLDYQVLTNIFEIQILTRFGISLNFNECVFCHRVGQAF
                     DFSFKYGACLCPEHYHEDERRCHLNPNIPYLLNQFQAIDFETLETISLKPGIKQELRQF
                     MDQLYEEYVGIHLKSKKFIDSLADWGQLLKEEKK"
     gene            3231..4223
                     /gene="plsX"
                     /locus_tag="EQH27_RS00305"
                     /old_locus_tag="EQH27_00310"
     CDS             3231..4223
                     /EC_number="2.3.1.274"
                     /GO_function="GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="plsX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717456.1"
                     /locus_tag="EQH27_RS00305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EQH27_00310"
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="WP_000717456.1"
                     /transl_table=11
                     /translation="MKKIAVDAMGGDYAPQAIVEGVNQALSDFSDIEVQLYGDEAKIKQ
                     YLTATERVSIIHTDEKIDSDDEPTRAIRNKKNASMVLAAKAVKDGEADAVLSAGNTGAL
                     LAAGFFIVGRIKNIDRPGLMSTLPTVDGKGFDMLDLGANAENTAQHLHQYAVLGSFYAK
                     NVRGIAQPRVGLLNNGTESSKGDPLRKETYELLAADESLNFIGNVEARDLMNGVADVVV
                     ADGFTGNAVLKSIEGTAMGIMGLLKTAITGGGLRAKLGALLLKDSLRGLKKQLNYSDVG
                     GAVLFGVKAPVVKTHGSSDAKAVYSTIRQIRTMLETDVVAQTAREFSGE"
     gene            4229..4462
                     /locus_tag="EQH27_RS00310"
                     /old_locus_tag="EQH27_00315"
     CDS             4229..4462
                     /NRPS_PKS="Domain: PP-binding (8-72). E-value: 3e-11.
                     Score: 35.5. Matches aSDomain:
                     nrpspksdomains_EQH27_RS00310_PP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136447.1"
                     /locus_tag="EQH27_RS00310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EQH27_00315"
                     /product="acyl carrier protein"
                     /protein_id="WP_000136447.1"
                     /sec_met_domain="PP-binding (E-value: 1.7e-10, bitscore:
                     38.3, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding_2 (E-value: 0.00059, bitscore:
                     17.1, seeds: 2, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTEKEIFDRIVTIIQERQGEDFVVTESLSLKDDLDADSVDLMEFI
                     LTLEDEFSIEISDEEIDQLQNVGDVVKIIQGK"
     aSDomain        4253..4444
                     /aSDomain="PP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_EQH27_RS00310_PP-binding.1"
                     /evalue="3.00E-11"
                     /label="EQH27_RS00310_PP-binding.1"
                     /locus_tag="EQH27_RS00310"
                     /protein_end="72"
                     /protein_start="8"
                     /score="35.5"
                     /tool="antismash"
                     /translation="RIVTIIQERQGEDFVVTESLSLKDDLDADSVDLMEFILTLEDEFS
                     IEISDEEIDQLQNVGDVVK"
     gene            <4499..4798
                     /locus_tag="EQH27_RS00315"
                     /old_locus_tag="EQH27_00320"
                     /pseudo=""
     CDS             <4499..4798
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /locus_tag="EQH27_RS00315"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="EQH27_00320"
                     /product="transposase family protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="LEMRNIGQAGKILSDSGYQGLMKIYPQAQTPRKSSKLKPLTVEDK
                     ACNHALSKERSKVENIFAKVKTFKMFSTTYRNHRKRFGLRMNLIAGIINHELGF"
     misc_feature    complement(4516..4531)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 20.17"
                     /tool="antismash"
     misc_feature    complement(4840..4853)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 22.31"
                     /tool="antismash"
     gene            5001..5231
                     /gene="blpU"
                     /locus_tag="EQH27_RS00320"
                     /old_locus_tag="EQH27_00325"
     CDS             5001..5231
                     /codon_start=1
                     /gene="blpU"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093487.1"
                     /locus_tag="EQH27_RS00320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EQH27_00325"
                     /product="bacteriocin-like peptide BlpU"
                     /protein_id="WP_001093487.1"
                     /sec_met_domain="Bacteriocin_IIc (E-value: 4.9e-16,
                     bitscore: 55.9, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNTKTMSQFEIMDTEMLACVEGGGCNWGDFAKAGVGGGAARGLQL
                     GIKTRTWQGAATGAVGGAILGGVAYAATCWW"
     gene            5234..5359
                     /locus_tag="EQH27_RS10950"
     CDS             5234..5359
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842754.1"
                     /locus_tag="EQH27_RS10950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="PncF family bacteriocin immunity protein"
                     /protein_id="WP_000346297.1"
                     /transl_table=11
                     /translation="MDFKSFIIGLVVGIFGPYMDDLIRKKFLKSSEKKTEKSVKK"
     gene            5770..5964
                     /locus_tag="EQH27_RS00325"
                     /old_locus_tag="EQH27_00330"
     CDS             5770..5964
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757183.1"
                     /locus_tag="EQH27_RS00325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EQH27_00330"
                     /product="hypothetical protein"
                     /protein_id="WP_000756776.1"
                     /transl_table=11
                     /translation="MKKVLKYFLVFVFLFLMNIFIFKILATLGFQLIMSEKSYIVLPLF
                     SLIVLYMIDKRIRKKKKSL"
     gene            5985..6878
                     /locus_tag="EQH27_RS00330"
                     /old_locus_tag="EQH27_00335"
     CDS             5985..6878
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343891.1"
                     /locus_tag="EQH27_RS00330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EQH27_00335"
                     /product="XRE family transcriptional regulator"
                     /protein_id="WP_000343891.1"
                     /transl_table=11
                     /translation="MDELKREIGYKIKTIRRSCGKTQIDICGDESELTIRQLARIENGQ
                     ALATLPKLLLIADKLGVSLQNIVDVKVIEIPKGFLKLKDELIHSQTYADKGRIERKEAI
                     LEEIYENYYENLPEEEQLIVDVTQARFDIYGSSDVTYGLGLVEEYFQQLLKKKYFSVND
                     LLIIELYFFCCAMGLEDKEHFEELAQKVLLCSEYEDKDSLVQMEKVLLSLFIQIQTEDS
                     LIYIQTFEKIIAKTRHVFYRPHLFLLKAKYALFVDKNVAEAESFYEKAISLAELLDDQV
                     LVQKILAEKQIDFPTT"
     gene            7421..7717
                     /locus_tag="EQH27_RS00335"
                     /old_locus_tag="EQH27_00340"
     CDS             7421..7717
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IId"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837727.1"
                     /locus_tag="EQH27_RS00335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EQH27_00340"
                     /product="uberolysin/carnocyclin family circular
                     bacteriocin"
                     /protein_id="WP_000837726.1"
                     /sec_met_domain="Bacteriocin_IId (E-value: 6.8e-21,
                     bitscore: 71.2, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKSKRMEFHNKFLIVSAMLAVISWLSLGVVSFPMLAGTLGISTKA
                     AATVVNLISAYSTVTAVISIVGAITGVGSIGSGIAATVLYILKKKGAAKAALW"
     gene            7724..8864
                     /locus_tag="EQH27_RS11020"
                     /old_locus_tag="EQH27_00345"
                     /pseudo=""
     CDS             7724..8864
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698829.1"
                     /locus_tag="EQH27_RS11020"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="EQH27_00345"
                     /product="hypothetical protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MKILSKKECESILGYLNFIGITLLFFVIISLSIYFKSYLPFQGKL
                     FLLFLVVDYATLNRYNMKLPIQSKKINTFYIRKNYIFLYLLAIVKKYYSFQILQSFIYL
                     FIYFVFSRYYWIFLFIHYSLFDSYSNYFFNST"
     gene            8861..9343
                     /locus_tag="EQH27_RS00340"
                     /old_locus_tag="EQH27_00350"
     CDS             8861..9343
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232094.1"
                     /locus_tag="EQH27_RS00340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EQH27_00350"
                     /product="stage II sporulation protein M"
                     /protein_id="WP_001232094.1"
                     /transl_table=11
                     /translation="MRKSGFLIYYVLFCIVCFLLGLLLSFILKNETFATIPPKLELQFT
                     LTVLQANSTNFLLYLFCFPISPLLQLFDLLSLGIQLGISGQMIGIVATIKGILPHALIE
                     IPNFIWYQGMSQYLLYRLLKDRGIKNILIYIKSNLKWYIISYLLLILAALIEGIKL"
     gene            9340..9936
                     /locus_tag="EQH27_RS00345"
                     /old_locus_tag="EQH27_00355"
     CDS             9340..9936
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687786.1"
                     /locus_tag="EQH27_RS00345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EQH27_00355"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_000687787.1"
                     /transl_table=11
                     /translation="MKIENESKSYDKCILKNISLEIDKGIYRVEGENGSGKSTLLQLLA
                     GLETFDSGLKNCVSKDVLYLDTNQLGVVPFTIEENLQLLCDSFQIHLSFEDKEQINTFF
                     DNKIGDNYMTASTGTKFKLGLSLIFAKNWDYIFIDETLSTVDARSIDMIAKRLISMKES
                     STIIYVSHNLLNQELISKSQRILIEEGRVYEISNK"
     gene            9917..10408
                     /locus_tag="EQH27_RS00350"
                     /old_locus_tag="EQH27_00360"
     CDS             9917..10408
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671992.1"
                     /locus_tag="EQH27_RS00350"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EQH27_00360"
                     /product="hypothetical protein"
                     /protein_id="WP_000671990.1"
                     /transl_table=11
                     /translation="MKFQTNNSLYKLMNLIIYLFLLYLFIDLLMKSGILDEQIDKLSEL
                     SNLFTIKIETILTVISVVFTFIATLIEYGFSLFLITLFFDKNKRNFGDILLSKNITLSF
                     SILLCYLFNPSDIKFAYFSVLTVLLIGITNYARFKNLRLTVVFSVPFLFNAIMSIINSF
                     "
     misc_feature    complement(10493..10506)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: strong, score: 24.43"
                     /tool="antismash"
     gene            10788..12941
                     /gene="comA"
                     /locus_tag="EQH27_RS00355"
                     /old_locus_tag="EQH27_00365"
     CDS             10788..12941
                     /codon_start=1
                     /gene="comA"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR01193"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668284.1"
                     /locus_tag="EQH27_RS00355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EQH27_00365"
                     /product="peptide cleavage/export ABC transporter ComA"
                     /protein_id="WP_000668286.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 4.4e-42, bitscore:
                     140.0, seeds: 36, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR01193 (E-value: 0.0, bitscore: 1123.1,
                     seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKFGKRHYRPQVDQMDCGVASLAMVFGYYGSYYFLAHLRELAKTT
                     MDGTTALGLVKVAEEIGFETRAIKADMTLFDLPDLTFPFVAHVLKEGKLLHYYVVTGQD
                     KDSIHIADPDPGVKLTKLPRERFEEEWTGVTLFMAPSPDYKPHKEQKNGLLSFIPILVK
                     QRGLIANIVLATLLVTVINIVGSYYLQSIIDTYVPDQMRSTLGIISIGLVIVYILQQIL
                     SYAQEYLLLVLGQRLSIDVILSYIKHVFHLPMSFFATRRTGDIVSRFTDANSIIDALAS
                     TILSIFLDVSTVVIISLVLFSQNTNLFFMTLLALPIYTVIIFAFMKPFEKMNRDTMEAN
                     AVLSSSIIEDINGIETIKSLTSESQRYQKIDKEFVDYLKKSFTYSRAESQQKALKKVAH
                     LLLNVGILWMGAVLVMDGKMSLGQLITYNILLVYFTNPLENIINLQTKLQTAQVANNRL
                     NEVYLVASEFEEKKTVEDLSLMKGDMTFKQVHYKYGYGRDVLSDINLTVPQGSKVAFVG
                     ISGSGKTTLAKMMVNFYDPSQGEISLGSVNLNQIDKKALRQYINYLPQQPYVFNGTILE
                     NLLLGAKEGTTQEDILRAVELAEIREDIERMPLNYQTELTSDGAGISGGQRQRIALARA
                     LLTDAPVLILDEATSSLDILTEKRIVDNLMALDKTLIFIAHRLTIAERTEKVVVLDQGK
                     IVEEGKHADLLAQGGFYAHLVNS"
     gene            12954..14303
                     /gene="comB"
                     /locus_tag="EQH27_RS00360"
                     /old_locus_tag="EQH27_00370"
     CDS             12954..14303
                     /codon_start=1
                     /gene="comB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801614.1"
                     /locus_tag="EQH27_RS00360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EQH27_00370"
                     /product="competence pheromone export protein ComB"
                     /protein_id="WP_000801632.1"
                     /transl_table=11
                     /translation="MKPEFLESAEFYNRRYHNFSSSVIVPMALLLVFLLGFATVAEKEM
                     SLSTRATVEPSRILANIQSTSNNRILVNHLEENKLVKKGELLVQYQEGVEGVQAESYAS
                     QLDMLKDQKKQLEYLQKSLQEGENHFPEEDKFGYQATFRDYISQEGSLRASTSQQNETI
                     ASQNAAASQTQAEIGNLISQTEAKIRDYQTVKSAIETGASLAGQNLAYSLYQSYKSQGE
                     ENPQTKVQAVAQVEAQISQLESSLATYRVQYAGSGTQQAYASGLSSQLESLKSQHLAKV
                     GQELTLLAQKILEAESGKKVQGNLLDKGKITASEDGVLHLNPETSDSSMVAEGALLAQL
                     YPSLEREGKAKLTAYLSSKDVARIKVGDSVRYTTTHDAGNQLFLDSTITSIDATATKTE
                     KGNFFKIEAETNLTSEQAEKLRYGVEGRLQMITGKKSYLRYYLDQFLNKE"
     misc_feature    14374..14387
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 22.31"
                     /tool="antismash"
     gene            14473..15180
                     /gene="purC"
                     /locus_tag="EQH27_RS00365"
                     /old_locus_tag="EQH27_00375"
     CDS             14473..15180
                     /EC_number="6.3.2.6"
                     /GO_function="GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene="purC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312208.1"
                     /locus_tag="EQH27_RS00365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EQH27_00375"
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="WP_000043303.1"
                     /transl_table=11
                     /translation="MSKQLIYSGKAKDIYTTEDENLIISTYKDQATAFNGVKKEQIAGK
                     GVLNNQISSFIFEKLNAAGVATHFVEKLSDTEQLNKKVKIIPLEVVLRNYTAGSFSKRF
                     GVDEGIALETPIVEFYYKNDDLDDPFINDEHVKFLQIAGDQQIAYLKEETRRINELLKV
                     WFAEIGLKLIDFKLEFGFDKDGKIILADEFSPDNCRLWDADGNHMDKDVFRRELGELTD
                     VYEIVWKKLQELK"
     gene            15191..15325
                     /locus_tag="EQH27_RS11025"
                     /old_locus_tag="EQH27_00380"
     CDS             15191..15325
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429437.1"
                     /locus_tag="EQH27_RS11025"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EQH27_00380"
                     /product="hypothetical protein"
                     /protein_id="WP_000429436.1"
                     /transl_table=11
                     /translation="MENLRLSTKRTQAEKVPQTFSLCRELGELTDVYEIVWEKLQGLK"
     misc_feature    complement(15396..15409)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 22.09"
                     /tool="antismash"
     misc_feature    16107..16121
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 21.98"
                     /tool="antismash"
     misc_feature    complement(16341..16355)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 21.93"
                     /tool="antismash"
     misc_feature    16867..16877
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 18.94"
                     /tool="antismash"
ORIGIN
        1 accgttttga ggttgcagat agaactgacg aagtcagctc aaaacaccgt tttgaggttg
       61 cagatagaac tgacgaagtc agtaacatat atacggcaag gcgacgttga cgtggtttga
      121 agagattttc gaagagtatt agaaaatgcc gataagggtc tgcataccaa ggctggtgag
      181 gatgatggca atccagcaga cggctccgag aacaatggat tttccactgg atttgaccat
      241 agcgaccaga ttagttttga gaccgatggc actcatggcc atgataatga ggaatttaga
      301 gagttgtttg agaggggtaa agaaactact agacacaccg agagaggtca gaagggtggt
      361 taggagcgat gcaaggatga agtaaaggat aaaaagtggg aagacttttt tcagttgtaa
      421 gccttgctta tttttttgct cgcgactttg ccagtaggag agaaagagag tgatggggat
      481 gatagctagg gtgcgcgtga gtttgacaat ggttgcggat tcgagggtat tggtctggta
      541 gagactgtcc caagcgctag ctgtggcggt tacagaggaa gtatcattga ccgcagttcc
      601 tgcaaagagg gcgaagccgt cattggatag atgaagccag gtgccgaggg ttggaaagat
      661 gagcgcagcc aagacattga agaaaaagat aacggagatg gcttgggcta cttccttttc
      721 cttagcatgg ataacaggcg ctgtcgctgc aatggcagaa cccccacaga tagaagaacc
      781 tactccaatc aaggtagcca gttttgtatc cagggcaaag aagcgctgga agaggtaggc
      841 aacaatcaag gctattgaaa tggtggaaag gatgacaggg agtgaagatt gcccaactgc
      901 gaagacttga gagatattga gaccaaaacc aagtaagata acggcatact ggagcaattt
      961 cttggaacta aaggtcaatc caacatccag ttgtttataa gacgagagaa agggatgtag
     1021 gagcatgccc ataaaaatcg caaaaacggg tgcgccaata acagggaaga atcctcctaa
     1081 gtaccaagat aggatggaaa tgagaaggca gaccaagatg cctgctccat tttttgatag
     1141 aaatgacata aaaacctcca aatagaatct gttaccatta tagacctgta aagaggaaaa
     1201 gtaaaataga aagtggaaag ctattctcta atgtattttt gcggtcgggg ggcttttgta
     1261 gtataataga gatacgtttt gaaagtagga ggtatctatg gacttaacta agcgctttaa
     1321 taaacagtta gataaaattc aagtttcgtt gattcgtcag tttgaccagg ctatttcgga
     1381 gattcctggg gtcttgcgtt tgaccttggg ggaacctgat tttacaacgc cagaccatgt
     1441 caaggaggcg gccaagcgag cgattgatca gaaccaatcc tactatacag ggatgagtgg
     1501 tctgctgact ctacgtcagg cagccagtga ctttgttaag gaaaagtacc aactggacta
     1561 tgctcctgaa aatgaaatct tggtcactat cggagctaca gaggctttat ctgcgacttt
     1621 gacggctatt ttggaagagg gagacaaggt gctcttgcca gctcctgctt atccgggtta
     1681 tgaaccgatt gtcaatctag ttggggcaga aattgttgag attgatacga ctgaaaatgg
     1741 ttttgtcttg actcctgaga tgttggagaa ggccattttg gagcagggtg ataagctcaa
     1801 ggcggttatt ctcaactatc cagccaatcc gacaggaatt acctacagtc gagagcagtt
     1861 agaggccttg gcagctgttt tacgcaagta cgaaattttt gttgtctgtg atgaggttta
     1921 ctcagaattg acctacacag gcgaagccca tgtgtctcta ggaacgatgt tgagagacca
     1981 ggctattatt atcaatggtt tgtctaaatc gcatgccatg acaggttggc gtttggggct
     2041 gatttttgct cctgcgacct tcacagccca gttaatcaag agtcaccagt acttggtcac
     2101 tgccgcaaat accatggcgc aacatgctgc ggtagaagcc ttgacggctg gtaaaaacga
     2161 tgcggagccc atgaagaagg aatatatcca acgtcgggac tatatcatcg aaaaaatgac
     2221 tgctcttggt tttgagatta tcaaaccaga cggtgccttc tatatttttg ctaaaattcc
     2281 agcgggctac aatcaagact cctttgcttt tctgaaggat tttgctcaga agaaggccgt
     2341 tgcctttatc cctggtgcag cctttggacg ttacggggaa ggctacgtcc gcctatctta
     2401 tgcagccagc atggagacta tcaaagaagc catgaaacga cttgaggagt acatgagaga
     2461 agcatgattc agtctatcac gagtcaaggc ttggtgcttt acaatcgcaa ttttcgtgag
     2521 gatgacaagc tcgtcaaaat ttttacagag caggttggca aacgcatgtt ttttgtcaaa
     2581 cacgctggtc agtctaagct ggcgcctgtt attcagccct tggtgctggc acgatttctc
     2641 ttgcgaatca atgatgacgg actcagttac atcgaagact atcatgaggt catgactttt
     2701 cccaagatta atagtgacct ctttgtcatg gcctatgcga cctatgtggc agctcttgca
     2761 gatgctagtt tgcaggacaa tcagcaggat gctcccttgt ttgctttttt gcaaaagact
     2821 ttggagttga tggaagcagg cttggattat caggttttga ccaatatttt tgaaattcaa
     2881 attttgactc gatttggaat cagcctcaat tttaatgagt gtgtcttctg ccatcgggtt
     2941 ggtcaggctt ttgacttttc tttcaaatat ggagcctgtc tctgtccaga gcattatcat
     3001 gaggatgaga gacgttgcca tctcaatccc aatatcccct atctgctcaa tcaatttcaa
     3061 gctattgatt ttgagacttt ggaaaccatt tcgctcaagc ctggaatcaa gcaagagcta
     3121 cgccaattta tggatcaact atatgaagag tacgttggga ttcacctaaa atcaaagaaa
     3181 tttattgatt ccctagcaga ctggggacaa ttactaaaag aggaaaagaa atgaaaaaaa
     3241 tcgcagtaga tgccatgggg ggcgattacg cacctcaggc catcgttgag ggtgtcaatc
     3301 aagccctatc tgacttttca gatatcgagg ttcaacttta cggagatgaa gctaaaatca
     3361 agcaatatct gacagcgaca gagcgcgtca gcattatcca tacggatgag aagattgatt
     3421 cggatgatga acctacgaga gctattcgga ataagaaaaa tgccagtatg gtattggcag
     3481 ccaaggctgt caaagatggt gaagcagacg ctgtcctttc ggctgggaat acaggtgcct
     3541 tgttggcagc aggattcttc atcgtgggtc gtatcaagaa tatcgaccgt cctggactca
     3601 tgtctacctt gcctaccgtt gatgggaaag gttttgacat gcttgacctc ggtgctaatg
     3661 cagaaaatac agcccagcac ctccatcaat acgctgtcct aggttctttc tatgccaaga
     3721 atgttcgtgg cattgcgcaa ccacgtgttg gtttgctcaa caacggaaca gagagtagca
     3781 agggcgaccc gcttcgtaag gaaacttatg aattactggc ggctgatgaa agtttgaact
     3841 ttatcggaaa cgtggaagcg cgtgatttga tgaatggcgt tgcagatgtt gttgtggcag
     3901 atggtttcac gggaaacgct gtgctcaaat ccatcgaagg gacagctatg ggaatcatgg
     3961 gcttgctcaa gacagctatt acaggtggtg gtcttcgagc gaaactaggt gccctccttc
     4021 tcaaggacag cctcagaggt ttgaaaaaac agctcaatta ttcagatgtt ggtggagcgg
     4081 tcttgtttgg tgttaaggca cctgttgtca agactcatgg ctcaagcgat gccaaggctg
     4141 tttatagtac gattcgccag attcgtacca tgctagaaac agacgtagtt gcccagactg
     4201 cgcgtgaatt ttcaggagaa taaaagagat gacagaaaaa gaaatttttg accgtattgt
     4261 gaccattatc caagagcgac agggagagga ctttgtcgtg acagaatcct tgagtctgaa
     4321 agacgatttg gatgcggatt ctgttgactt gatggagttt atcttgactc tggaagatga
     4381 atttagtatc gaaatcagcg atgaagaaat tgaccaactc caaaacgtag gagatgtggt
     4441 taaaatcatt caaggaaaat agcaatcgga gttccaagtc aacggaagta gatggttttt
     4501 agaaatgaga aatatcggac aagctggtaa aatcttgtct gacagtggtt atcaagggct
     4561 catgaagata tatcctcaag cacaaactcc acgtaaatcc agcaaactca agccgctaac
     4621 agttgaagat aaagcctgta atcatgcgct atctaaggag agaagcaagg ttgagaatat
     4681 ctttgccaaa gtaaaaacgt ttaaaatgtt ttcaacaacc tatcgaaatc atcgtaaacg
     4741 cttcggatta cgaatgaatt tgattgctgg tattatcaat catgaactag gattctagtt
     4801 ttgcaggaag tctaatagta aaaaagtgat tagaaaacat cttttttaaa aatagagatg
     4861 attttgaaac aaaaaagcta attcaagacg tttcgatgcc aattcaagat ttggatgaaa
     4921 aaaattaata gatactgtta tactaaactt gtcaagtttg taacaagaca aatattaaaa
     4981 ataaaaaaga ggtattcgtt atgaatacaa aaacgatgtc acaatttgaa attatggata
     5041 ctgagatgct tgcttgcgtt gaaggtggcg gatgcaattg gggagatttt gccaaagcag
     5101 gtgttggagg aggagcagca cgaggtcttc agctaggaat taaaacaaga acatggcaag
     5161 gtgcagcaac tggtgctgtg ggaggagcta tacttggagg tgtggcctat gcagcgacat
     5221 gttggtggta attatggatt ttaaaagttt tattattggt ttagtagttg gtatatttgg
     5281 tccttatatg gatgatttaa ttagaaaaaa atttttaaag tcttcggaga agaaaacaga
     5341 aaaatctgtt aaaaaataat caaaactata aatgatgaat ctgaatcaaa attattttgc
     5401 gcatgtaaat aggagtctta tagtaacgag tcaaaaaagg agtaactatg aatcgtaatt
     5461 tagaacggtg ttatctattc tgactaggaa tagatcatac cagaggtggc ttagaaatag
     5521 cggggacatt agaaattgaa ggaactttaa ataggatgtc gtaaatgttg ctatcaatga
     5581 tttatttgtc ccaagcttgc ctagggtgtc agtaaaaaat cattttcctt tcatatcata
     5641 tttttagtag gtaggatgtt tgttctgtct attttttgcc aaaaaaagtg cagttgaagt
     5701 gtttatttgg agttttatat atattttcga taaaataggt gtagaaatta ggaaagttga
     5761 agaaaagaaa tgaagaaggt tttaaaatat tttttagtat ttgtttttct atttttgatg
     5821 aatattttca tctttaagat actagcaacc ctgggatttc agctgataat gagtgaaaag
     5881 agttatattg ttctaccgct gttttcgctt atcgttttat atatgattga caaaagaatt
     5941 aggaagaaaa agaaatcttt gtaatcaact taaggagtat tactatggat gagttgaaac
     6001 gtgaaattgg ttataaaatc aaaactatta ggcgatcttg tggaaaaact cagatagata
     6061 tttgtggtga tgaatcagag ttgacgattc gtcagctagc acgtattgaa aatgggcaag
     6121 ctttagcaac acttcctaaa ttattattga ttgcggataa acttggagta tcattacaga
     6181 atattgtgga tgttaaggtt atagagattc caaaaggatt cctaaaactc aaagatgagc
     6241 tgattcattc tcaaacctac gctgataagg gaagaatcga acgaaaagaa gctatacttg
     6301 aggaaatata tgaaaactat tatgagaatt tgcctgagga agagcagtta attgtggatg
     6361 tgacacaagc tcgttttgat atttatggta gttcagatgt gacctacggt ttagggttag
     6421 tggaagaata tttccagcag ttattaaaaa agaaatattt ttctgtaaat gatttgttga
     6481 ttattgaatt gtatttcttt tgttgtgcta tgggattaga agataaagaa cattttgagg
     6541 agttagctca aaaagtattg ctatgttcag aatatgaaga taaggatagc ctagttcaga
     6601 tggaaaaagt tcttctgagc ctatttatcc aaattcagac agaagatagt ctgatatata
     6661 ttcaaacatt tgagaaaatt attgctaaaa caagacatgt tttttaccga cctcatctat
     6721 ttttgttaaa ggcgaaatat gctttatttg tagataagaa tgtggcagag gcagaaagtt
     6781 tctatgaaaa ggcgatttct ctagcagaat tgctagatga tcaggtatta gttcagaaaa
     6841 ttttagcaga aaaacaaata gatttcccaa caacctgact tctaagtcat gttgtttttt
     6901 tcatgttttt gttatactaa actcataaag aaaacgattc caaaaagaaa ggaagtttat
     6961 tatggttgga acgttgtttg tccttttaag tataggagtc tggttctatc tataatatca
     7021 ggtagagatt aatggcataa ggtattccta tattggatta aaggaatgtt ttaataggtt
     7081 tcctacatta gtatagagga tgaaactcaa ttttcaatga tatagaagag ttatttggag
     7141 attgtgaggg ttgagtatgt ttatttatga tgtaggaagt gtaaactaaa aaggaggtag
     7201 taatcgtata aaagttaaat agacattatc ttttattgag tcttagtgat ataaaagtaa
     7261 tttgatagtc taggaaaatg tagtagtaat ttatttatta tagatatgtt aataaaagta
     7321 gtataaaata tagacgaaca tttgaagaga gttaggaata caacatgtgc gaattaactc
     7381 tcaataaaac atacataata tacaaaagga gtacactaaa atgaaatcta aaagaatgga
     7441 atttcataat aaatttctta ttgtttcagc aatgttagca gtcattagtt ggttaagctt
     7501 aggtgttgtt tcttttccta tgttggctgg gacattagga atttcgacaa aagctgctgc
     7561 tactgtagtc aatttgatta gtgcttatag tacagttaca gcagtaattt caattgtagg
     7621 tgcaatcaca ggagtaggtt ctattggttc gggtattgca gcaacagttc tttatatatt
     7681 gaaaaagaaa ggtgctgcta aagcagctct atggtagaac tagatgaaaa tactttctaa
     7741 aaaagaatgt gaaagtattt taggttatct taattttatt gggataaccc ttttgttttt
     7801 tgttatcata agtttgtcaa tctattttaa aagctatctt ccttttcaag ggaaattatt
     7861 tttacttttt ttggtagtag attacgctac tttgaatcgt tataatatga aactgcctat
     7921 ccaaagtaaa aaaataaata ctttctatat aaggaaaaac tatatttttc tctatcttct
     7981 tgccattgtc aagaaatatt attctttcca aattttacaa tcattcattt atttatttat
     8041 ttattttgta ttttctagat attattggat atttttattt atccattact ctttgtttga
     8101 tagctattca aattatttct ttaattctac ctagtttgtt ttcaattata agtaaattgt
     8161 tcttattttt atctatagga ctattgtcta tagaacaagg acaagttgta atttatgtca
     8221 tactactcaa ttattttatt ttagtcatgg ggatattaga taatcatgcg agtaatttct
     8281 attttaaaag cagtcgaatg agtcaaaaaa gatttcgtag cagtctattt atattgcttc
     8341 gtttttgttg gttaaataaa gggttaactt tttcttttat tttgattagt attcttcttt
     8401 cctatctatt ttggtatatg atgacgatgt tatcaattga tgacacaaaa ataccaatat
     8461 tatttcttta tacctcaata tatattacat taatggaggc aataataggg aatcataaat
     8521 cctcaattgg ggtagatcta gctttacttc aattatctgc tcttaatcat tctaaaatga
     8581 atttcttttt taagaatgca caactaatga tgttgacgca tttaatctct ttcttacaat
     8641 tttcattgtt gttatgtttg attccggtga caagttatag taatgatata aaaagtattt
     8701 tgttgaattt gttattgatt ccattcaatt attttatagc ctatgtctat tataagaaag
     8761 ctctattttt aattaaaggt acagttagtt tattgagatg gagttttctg gttttatatt
     8821 gttttctgat aatatttgta attttagtag ggggcattag atgagaaaaa gtggttttct
     8881 gatttactat gtgttatttt gtatagtctg ttttttacta ggtttacttt taagctttat
     8941 attaaaaaat gagacttttg ctactatacc tccgaaactg gaattacaat ttacattgac
     9001 agttttacag gcaaattcta caaatttttt gctatatcta ttctgttttc cgatttcgcc
     9061 cttattgcaa ctttttgatt tgctatcttt aggaatacaa ttgggtatat ctggtcagat
     9121 gattggaata gtagctacaa taaaaggaat attacctcat gctcttattg aaataccgaa
     9181 ttttatttgg tatcaaggta tgagtcagta tcttttatac cgattattga aggatagggg
     9241 aattaaaaat atacttattt atataaaaag taatttaaaa tggtatatca tttcttatct
     9301 tttattaatt ttagcagcgt tgatagaggg aattaaatta tgaaaattga aaatgaatct
     9361 aaaagttatg ataaatgtat attgaaaaat atatcactag agattgataa gggaatttac
     9421 agagttgaag gagagaatgg ttcaggaaag agcacccttc tacaactttt agctgggcta
     9481 gagacttttg atagtggttt aaagaattgt gtctccaaag atgtactata ccttgatacc
     9541 aatcagcttg gggttgttcc atttactata gaagaaaatt tgcagttgtt gtgtgattct
     9601 tttcaaatcc atctatcatt tgaggataaa gaacaaatta atactttttt tgataataaa
     9661 atcggagata actatatgac agcatctact ggaacaaaat tcaaattagg cttatcctta
     9721 atttttgcta aaaattggga ttatattttt attgatgaga ctttatcaac agtggatgca
     9781 agaagtattg atatgattgc taaacgctta atttccatga aagaaagctc tactattata
     9841 tatgtatctc ataatttatt gaatcaagaa ttgattagca aatctcaaag gattttaata
     9901 gaggaaggac gggtttatga aatttcaaac aaataatagt ttatacaaat taatgaatct
     9961 gattatttat ctttttcttt tatatttgtt tattgattta ctgatgaagt cgggaatttt
    10021 agatgaacaa attgataaat tatctgaact atctaatctt tttacaatta aaatagaaac
    10081 aatattgaca gtgatttctg ttgttttcac atttatagca actcttattg aatatggatt
    10141 ttctctcttt ctgattacat tattttttga taagaataag aggaactttg gagatattct
    10201 tttatctaaa aatataacct taagttttag cattttactt tgttatttat ttaatccatc
    10261 ggatataaag tttgcctatt tcagcgtatt gacagtatta ctaattggga taacaaatta
    10321 tgcgagattt aaaaatttaa gattgacagt agtgttttct gtaccatttt tatttaatgc
    10381 tataatgtct attataaata gtttttagaa atactaaaat ttttttcttt acttaataat
    10441 gatatgaaat catttcggtt ttgttaactt gtaattctta tattttctag gtagaacgtt
    10501 tgttctgcct atttttttat ccaaaaagtg cagttgggag ggagataggc tcatttggga
    10561 aggaagtcca gtttttgttt agtgattggg gtaagatagt tgttatcaga tgagtttata
    10621 ctcttcgaaa atcaaattca aaccacgtca acgtcgcctt gccgtatata tgtgactgac
    10681 ttcgtcagtc ctatctacaa cctcaaaaca gtgttttgag cagcctgcgg ctagtttcct
    10741 agtttgctct ttgattttca ttgagtatta gggaaaagga gatgaatatg aaatttggga
    10801 aacgtcacta tcgtccgcaa gtggatcaga tggactgcgg tgtagcttca ttagccatgg
    10861 tttttggcta ctatggtagt tattattttt tggctcactt gcgagaattg gctaagacga
    10921 ccatggatgg gacgacggct ttgggcttgg tcaaggtggc agaggagatt ggttttgaga
    10981 cgcgagccat taaggcggat atgacgcttt ttgacttgcc ggatttgact tttccttttg
    11041 ttgcccatgt gcttaaggaa gggaaattgc tccactacta tgtggtgact gggcaggata
    11101 aggatagcat tcatattgcc gatccagatc ccggggtgaa gttgactaaa ctgccacgtg
    11161 agcgttttga ggaagaatgg acaggagtga ctctttttat ggcacctagt ccagactata
    11221 agcctcataa ggaacaaaaa aatggtctgc tctcttttat ccctatatta gtgaagcagc
    11281 gtggcttgat tgccaatatc gttttggcaa cactcttggt aaccgtgatt aacattgtgg
    11341 gttcttatta tctgcagtct atcattgata cctatgtgcc agatcagatg cgttcgacac
    11401 tggggattat ttctattggg ctagtcatcg tctacatcct ccagcaaatc ttgtcttacg
    11461 ctcaggagta tctcttgctt gttttggggc aacgcttgtc gattgacgtg attttgtcct
    11521 atatcaagca tgtttttcac ctccctatgt ccttctttgc gacacgcagg acaggggata
    11581 tcgtgtctcg ttttacagat gctaacagta ttatcgatgc gctggcttcg accattcttt
    11641 cgattttcct agatgtgtca acggtcgtca ttatttccct tgttttattt tcacaaaata
    11701 ctaatctctt tttcatgact ttattggcac ttcctatcta tacagtgatt atctttgcct
    11761 ttatgaagcc gtttgaaaag atgaatcggg acaccatgga agccaatgcg gttctgtctt
    11821 cttctatcat tgaggacatc aacggtattg agactatcaa gtccttgacc agtgaaagtc
    11881 agcgttacca aaaaattgac aaggaatttg tggattatct gaagaaatcc tttacctata
    11941 gtcgagcaga gagtcagcaa aaggctctga aaaaggttgc ccatctcttg cttaatgtcg
    12001 gcattctctg gatgggggct gttctggtca tggatggcaa gatgagtttg gggcagttga
    12061 ttacctataa tatcttgctg gtttacttta ccaatccttt ggaaaatatc atcaatctgc
    12121 aaaccaagct tcagacagcg caggttgcca ataaccgtct aaatgaagtg tatctagtag
    12181 cttctgagtt tgaggagaag aaaacagttg aggatttgag cttgatgaag ggagatatga
    12241 ccttcaagca ggttcattac aagtatggct atggtcgaga cgtcttgtcg gatatcaatt
    12301 taaccgttcc ccaagggtct aaggtggctt ttgtggggat ttcagggtca ggtaagacga
    12361 ctttggccaa gatgatggtt aatttttacg acccaagtca aggggagatt agtctgggta
    12421 gtgtcaatct caatcagatt gataaaaaag ccctgcgcca gtacatcaac tatctgcctc
    12481 aacagcccta tgtctttaac ggaacgattt tggagaatct tcttttggga gccaaggagg
    12541 ggacgacaca ggaagatatc ttacgggcgg tcgaattggc agagattcga gaggatatcg
    12601 agcgcatgcc actgaattat cagacagaat tgacttcgga tggggcaggg atttcaggtg
    12661 gtcaacgtca gagaatcgct ttggcgcgtg ctctcttgac agatgcgccg gtcttgattt
    12721 tggatgaggc gactagcagt ttggatattt tgacagagaa gcggattgtc gataatctca
    12781 tggctttgga caagaccttg attttcattg ctcaccgctt gactattgct gagcggacag
    12841 agaaggtagt tgtcttggat cagggcaaga ttgtcgaaga aggaaagcat gctgatttgc
    12901 ttgcacaggg tggcttttac gcccatttgg tcaatagcta gaaagaggag aggatgaaac
    12961 cagaattttt agaaagtgcg gagttttata atcgtcgtta ccataatttt tccagtagtg
    13021 tgattgtacc catggccctt ctgctcgtgt ttttacttgg ctttgcaact gttgcagaaa
    13081 aggagatgag tttgtccact agagctactg tcgaacctag tcgtatcctt gcaaatatcc
    13141 agtcaactag caacaatcgt attcttgtca atcatttgga agaaaataag ctggttaaga
    13201 agggggagct tttggttcaa taccaagaag gggtagaggg tgtccaagcg gagtcctatg
    13261 ccagtcagtt ggacatgcta aaggatcaaa aaaagcaatt ggagtatctg caaaagagcc
    13321 tgcaagaagg ggagaaccac tttccagagg aggataagtt tggctaccaa gccacctttc
    13381 gcgactacat cagtcaagaa ggcagtctta gggctagtac atcgcaacaa aatgagacca
    13441 tcgcgtccca gaatgcagca gctagccaaa cccaagccga aatcggcaac ctcatcagtc
    13501 aaacagaggc taaaattcgc gattaccaga cagttaagtc agctattgaa acaggtgctt
    13561 ccttggccgg tcagaatcta gcctactctc tttaccagtc ctataagtct cagggcgagg
    13621 aaaatcccca aactaaggtt caggcagttg cacaggttga agcacagatt tctcagttag
    13681 aatctagtct tgctacttac cgtgtccagt atgcaggttc aggtacccag caagcctatg
    13741 cgtcagggtt aagcagtcaa ttggaatccc ttaaatccca acacttggca aaggttggtc
    13801 aggaattgac ccttctagcc cagaaaatct tggaggcaga gtcaggtaag aaggtacagg
    13861 gaaatctttt agacaagggg aaaattacgg caagtgagga tggggtgctt catcttaatc
    13921 ctgagaccag tgattctagc atggttgcag aaggtgccct actagcccaa ctttatccat
    13981 ctttggaaag agaagggaaa gccaaactca cagcctatct aagttcaaaa gatgtagcaa
    14041 gaatcaaggt cggtgattct gttcgctata ctacgactca tgatgccggg aatcaacttt
    14101 ttctagattc tactattaca agtattgatg cgacagctac taagactgag aaagggaatt
    14161 tctttaaaat cgaggcggag actaatctaa cttcggagca ggctgaaaaa cttaggtacg
    14221 gggtggaagg ccgtctacag atgattacgg gcaagaaaag ttatctacgt tattatttgg
    14281 atcaattttt gaacaaagag taatgttcgt gtttttagag ttaaataatt tttaaactgt
    14341 gagaaagatt cttcttgcag ttttttcttt acaattttta aaaaacatct gctatttatt
    14401 cggttaaatt cttgtgtttt ttggtttttt gtgataaaat gtgctcaagt aatacgaaag
    14461 gcgaacttta aaatgtcaaa acaattgatc tattcgggaa aagctaaaga tatctataca
    14521 actgaggatg aaaatcttat tatttcaact tacaaggacc aggcgactgc tttcaacggt
    14581 gtcaagaagg agcagattgc aggtaaggga gtcttgaata atcagatctc atcttttatt
    14641 tttgagaaat taaatgcggc tggtgtggcg actcactttg tggagaaact ttcagacacg
    14701 gaacaactca ataaaaaggt taagattatt cctttggaag tcgtgctccg caactatact
    14761 gctggttcct tttcaaaacg ttttggtgtg gatgagggaa tcgccttgga gactccgatt
    14821 gtcgaatttt actacaaaaa tgatgatttg gatgatccat ttatcaatga tgagcatgtg
    14881 aaattcctac agattgcggg tgaccagcag attgcctact tgaaggaaga aacgcgtcgt
    14941 atcaatgaac tattgaaagt ctggtttgct gagattgggc ttaaattgat tgactttaag
    15001 ctagagttcg gttttgacaa ggatggcaag attatcttgg cagacgaatt ttcaccagat
    15061 aactgccgct tgtgggacgc tgatggcaac cacatggata aggatgtttt ccgtagagaa
    15121 ttgggagaac taaccgacgt ttatgagatt gtttggaaaa agttgcagga attgaaataa
    15181 tctgtttgca atggaaaacc ttcgtctctc aactaaaagg actcaggctg aaaaggttcc
    15241 ccagaccttt tcactctgta gagaactagg tgaactaacc gatgtttacg aaattgtctg
    15301 ggaaaagttg cagggtttaa aataacaacc tcaaggctgt ttgggaatat tgcaagagct
    15361 gaaataaagg aataagaatt gatggataaa cgtatttttg ttgaaaaaaa ggctgatttt
    15421 caggtcaagt cagagagttt ggttagagaa ctccagcaca acttgggact atcaagcttg
    15481 aaaagtattc gtattgtgca agtatatgat gtatttgact tggttgagga cttgtttgca
    15541 cctgcagaga agcacatttt ctctgagcag gtaaccgacc atgttttaga tgaagtatct
    15601 gtgcaggcgg atcttgctaa ctatgctttc tttgccattg aaagtctgcc agggcagttt
    15661 gaccagcgtg cagcttcgtc acaggaagcc ttgcttttgt tgggaagttc gagtgacgtg
    15721 acagtcaaca cagcccaact ttacttggtg aataaagata ttgatgcgac tgagttggaa
    15781 gctgtcaaaa actacctgct caatccagtt gattctcgtt tcaaggatat cacgacaggg
    15841 attgccaagc aggagttttc agagtcagac aagaccattc caaaattgac tttctttgaa
    15901 agctatacag cagaagactt tgctcgctac aaggccgagc aagggatggc catggaagtg
    15961 gatgatttgc tctttatcca agactacttt aagtcaatcg ggcgcgtgcc aactgagact
    16021 gaactcaagg ttttggacac ttactggtct gaccactgcc gtcatacgac ttttgagaca
    16081 gagttgaaac acatcgactt ttcagcttct aaatttcaaa agcaattgca gtcaacctat
    16141 gacaagtata ttgccatgcg cgaggagtta ggtcggtctg aaaaaccaca aaccttgatg
    16201 gatatggcga ctattttcgg tcgttatgag cgtgctaatg gacgattgga tgatatggaa
    16261 gtctctgacg aaatcaatgc ctgctcagtt gaaattgaag tggatgttga tggtgtcaag
    16321 gaaccttggc tcctcatgtt taaaaatgaa actcacaacc atccaacaga aattgagcca
    16381 tttggtggag cggctacctg tatcggtgga gctattcgtg atccgttgtc aggccgttcc
    16441 tatgtttacc aagccatgcg tatttcaggt gctggtgata ttacagcacc gatttcggaa
    16501 actcgtgctg ggaaattgcc acaacaagtc atttctaaaa cagcagctca tggttattct
    16561 tcatatggta accagattgg gcttgccaca acctacgttc gtgaatactt ccacccaggc
    16621 tttgtagcta aacgtatgga acttggtgcc gttgttggtg cggctcccaa gggcaatgtt
    16681 gtccgtgaaa aacctgaagc aggtgatgtg atcatccttc tcggaggcaa aacaggtcgt
    16741 gatggtgtcg gtggtgcgac gggctcttct aaggttcaaa cagttgagtc tgtagagact
    16801 gctggtgctg aggttcaaaa aggaaatgcc atcgaagaac gcaagattca gcgcctcttc
    16861 cgtaatggca atgtcacttg tctgatcaag aagtccaatg actttggggc aggcggcgtc
    16921 tgtgtggcta tcggtgaatt ggcagacggt cttgaaatcg acctcaacaa ggtgcctctt
    16981 aaataccagg gcttgaatgg tacagaaatt gccatctctg aatcacaaga acggatggcg
    17041 gtcgtggttc gtcctgaaga tgtggatgcc ttcgttgccg aatgtaacaa agaaaatatt
    17101 gatgctgttg tggtggcgac agtaactgaa aaaccaaatc ttgtcatgca ctggaatggt
    17161 gagacaatcg ttgacttgga gcgtcgtttc cttgacacca atggtgtgcg cgtggttgtc
    17221 gatgccaaag ttgtggacaa ggatgtcaaa ctcccagaag agcgtcaaac atctgctgaa
    17281 acactggaat cagataccct tacggttcta tctgacctca accatgcaag tcaaaaagga
    17341 ttacagacta tttttgactg ctctgttgga cgctcaacgg ttaatcaccc acttggtggt
    17401 cgttaccaac tcacaccaac tgaggcatct gtgcagaaat tgccagttca acacggtgtg
    17461 actcatactg cgtcggtcat tgctcaaggt ttcaacccat atgtagctga atggtctcca
    17521 taccacggtg ctgcttacgc ggttatcgaa gcaactgctc gtttggtggc tgctggtgcc
    17581 aactggtcta aggctcgttt ctcttaccaa gaatatttcg agcggatgga caagcaagca
    17641 gagcgtttcg gtcagccagt agccgccctt ctaggctcta ttgaagctca gattcaactt
    17701 ggcttgccat ctatcggtgg taaggactcc atgtctggta cctttgaaga attgaccgtt
    17761 ccgccaacct tggttgcctt tggggtgacg acggcagata gccgtaaggt gctctctcca
    17821 gaatttaaag ctgttgggga aaatatctac tacatcccag gtcaagccct ctctgcagag
    17881 attgattttg acttgattaa gaaaaatttt gctcagtttg aagcaagcca agctgaccat
    17941 a
//
