LOCUS       NZ_CP091381            10393 bp    DNA     circular CON 18-AUG-2024
DEFINITION  Amycolatopsis sp. FU40 chromosome, complete genome.
ACCESSION   NZ_CP091381
VERSION     NZ_CP091381.1
KEYWORDS    RefSeq.
SOURCE      Amycolatopsis sp. FU40
  ORGANISM  Amycolatopsis sp. FU40
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Amycolatopsis.
REFERENCE   1  (bases 1 to 9944196)
  AUTHORS   Reisman,B.J. and Bachmann,B.O.
  TITLE     Biosynthesis of Apoptolidin Family Glycomacrolides
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9944196)
  AUTHORS   Reisman,B.J. and Bachmann,B.O.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2022) Chemistry, Vanderbilt University, 1234
            Stevenson Center Drive; Room 7330, Nashville, TN 37235, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 23-JAN-2022
            Assembly Method                   :: Flye v. 2.9
            Assembly Name                     :: NFU40_flye_2.9
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 47.0x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_021903635.1-RS_2024_08_18
            Annotation Date                   :: 08/18/2024 04:30:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,491
            CDSs (total)                      :: 9,417
            Genes (coding)                    :: 9,360
            CDSs (with protein)               :: 9,360
            Genes (RNA)                       :: 74
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 7 of 57
            Pseudo Genes (incomplete)         :: 50 of 57
            Pseudo Genes (internal stop)      :: 5 of 57
            Pseudo Genes (multiple problems)  :: 5 of 57
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:13:09
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6884671
            Orig. end                         :: 6895064
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP091381.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Brian O. Bachmann,
            Vanderbilt University.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10393
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="34"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10393
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10393
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5393](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5393)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            complement(917..3670)
                     /locus_tag="L3Q65_RS32315"
                     /old_locus_tag="L3Q65_32315"
     CDS             complement(917..3670)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1041:
                     transcriptional regulator, SARP family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007033452.1"
                     /locus_tag="L3Q65_RS32315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L3Q65_32315"
                     /product="AfsR/SARP family transcriptional regulator"
                     /protein_id="WP_237597432.1"
                     /transl_table=11
                     /translation="MAGGGARVELLGPVRLLDGSRAVPVGGPGVRGLLALLALRVGKVV
                     PLEEIIDALWGHDPPATARTIVHGNVSQLRRLLREMADPVAEISTRPPGYQLSLAPDRI
                     DAHRARALLDQAALESPESASKLLAEALALWQGPTLSGVPDSVRAPELEELRRAIRYAR
                     INADLDLGRYDELIVELSPIVRADPLAERIAGQLMRALYHAGRRGEALELYRTVSRATL
                     GSLGVEPGADLRSLHERVLNDDLAAHESAVPEQAVAPSQLPPALPTLAGRETEVEWLDG
                     LAERGDSTVIVSGTAGVGKSALVIWWAHRAARLFPDGVLYAALQGFDPHQPPLEPGDLL
                     SQFLLGLGVPADEVPEQVHERVALYRSLIAGRRMLVLLDDARSAEQVRPLLPPGARSMT
                     VVTSRSRLEALAVSHAAKLRVLGTLAPEDAVSVIEELAGPADFDLNHALARLCGYLPLA
                     LRIAGAKLAASPQWTLKDLVGELGNERTRLAGLDVTGADDGVRAAFDVSFRGLPDGLAR
                     TLLRLGAVPARHTGPHLTAAIAQIPVQQARRELRTLAAHNLIAETSRDAFAPHDLVRLY
                     LRELAETELEPAERAAVLDRAIRYHQAASDTARRKMLRIVDPLDLTGLVPPDALPPLET
                     FAQAQDWFAAEWPNLLAVLDAAHADGRYADVWRLARVAHTYRVVCPLYEEWIRLVDLGL
                     AAAEAADEPLGQCWMLISRCAIALTFELSQASIADAERAVGLATRLDGRSLIVAKIHLG
                     SVLTLLGRYSEAIERLLEAVAETERTGDLELRGQALNNCAETEKRAGRPAEAITHQLAS
                     LEIDRELGDDSYAVVSLNNLAELHLGIGDLEAAERYAREAVSLAVARQFELQEGILRVT
                     LARILRGQERLELAREQYRRAVALYERMNPRQVEDLRKEAADLGDQ"
     gene            complement(4032..4934)
                     /gene="thpD"
                     /locus_tag="L3Q65_RS32320"
                     /old_locus_tag="L3Q65_32320"
     CDS             complement(4032..4934)
                     /EC_number="1.14.11.55"
                     /GO_function="GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005441527.1"
                     /locus_tag="L3Q65_RS32320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L3Q65_32320"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_182896437.1"
                     /sec_met_domain="PhyH (E-value: 2.9e-33, bitscore: 115.0,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTIMDTRVEDGYPTRITGTPEHLPRVHPTVWGTEADGPIDAATLA
                     NHDAKGYTVVEGLLSPGEVQTYWQEVVRLSSDEKLRDDERVITEARTGEVRSIFDVHEI
                     SGLIAELVRDPRVLDRARQILGSEVYIHQSRINYMPGFKGTGFYWHSDFETWHAEDGMP
                     SPRAVSCSIALTDNYPYNGGLMVMPGSQRTFVQCVGETPDANYKSSLKDQRVGVPTNDD
                     ITKLAADCGIEQFTGSAGSALWFDSNIMHGSSNNITPYPRSNIFLVFNSVENALQEPFA
                     ASAPRPSFIAGRNSAPITR"
     gene            complement(5001..5393)
                     /locus_tag="L3Q65_RS32325"
                     /old_locus_tag="L3Q65_32325"
     CDS             complement(5001..5393)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003071625.1"
                     /locus_tag="L3Q65_RS32325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L3Q65_32325"
                     /product="ectoine synthase"
                     /protein_id="WP_037807993.1"
                     /sec_met_domain="ectoine_synt (E-value: 4e-54, bitscore:
                     180.9, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLVRTLDEITDTDADIKTPNWRSKRIILAKEGVGFSVHETTLYAG
                     TVNDFWYANHIEAVFITSGEGEIEDLATGEVHQLKPGTLYLLNDHDKHQVRPKTEIKCV
                     CVFNPPVTGREVHDENGVYPLVTEDS"
     gene            complement(5425..6675)
                     /gene="ectB"
                     /locus_tag="L3Q65_RS32330"
                     /old_locus_tag="L3Q65_32330"
     CDS             complement(5425..6675)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0047307 - diaminobutyrate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (21-357). E-value: 3.6e-72.
                     Score: 235.1. Matches aSDomain:
                     nrpspksdomains_L3Q65_RS32330_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009084079.1"
                     /locus_tag="L3Q65_RS32330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L3Q65_32330"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_182896436.1"
                     /sec_met_domain="Aminotran_3 (E-value: 8.8e-74, bitscore:
                     247.6, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSIFEELESEVRSYSRGWPVVFDRAQGSRLYSEDGKAYLDFFAGA
                     GALNYGHNNPHLKKALIDYIQRDGVTHALDMYTVAKRDFLETFREKILAPRELDYKIVF
                     PGPGGANAVEAALKLARKVTGKESVINFTNAFHGMTLGALSVTGNSMKRGAAGIPLVHA
                     TPMPYDKYFDGAMPDFMYFEKLLEDSGSGLNEPAAVIVEGVQGEGGINAARLEWLKGLD
                     DLCKRHGILLILDDVQMGCGRTGPFFSFEDAGISPDIVCLSKSIGGYGLPMALTLIKPE
                     LDVWEPGEHNGTFRGISPAFVTATEALRVYWSDDELEKSTKAKGERIASTFKDLVESYP
                     EANLVAKGRGLARGLEFGSGELAGAVCKAAFERGLLMETSGPDGEVMKLLPPLTLTDEE
                     LSEGLAIITESVAAVLK"
     aSDomain        complement(5605..6612)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_L3Q65_RS32330_Aminotran_3.1"
                     /evalue="3.60E-72"
                     /label="L3Q65_RS32330_Aminotran_3.1"
                     /locus_tag="L3Q65_RS32330"
                     /protein_end="357"
                     /protein_start="21"
                     /score="235.1"
                     /tool="antismash"
                     /translation="FDRAQGSRLYSEDGKAYLDFFAGAGALNYGHNNPHLKKALIDYIQ
                     RDGVTHALDMYTVAKRDFLETFREKILAPRELDYKIVFPGPGGANAVEAALKLARKVTG
                     KESVINFTNAFHGMTLGALSVTGNSMKRGAAGIPLVHATPMPYDKYFDGAMPDFMYFEK
                     LLEDSGSGLNEPAAVIVEGVQGEGGINAARLEWLKGLDDLCKRHGILLILDDVQMGCGR
                     TGPFFSFEDAGISPDIVCLSKSIGGYGLPMALTLIKPELDVWEPGEHNGTFRGISPAFV
                     TATEALRVYWSDDELEKSTKAKGERIASTFKDLVESYPEANLVAKGRGLARGLEF"
     misc_feature    complement(6673..6684)
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: weak, score: 16.51"
                     /tool="antismash"
     gene            complement(6717..7211)
                     /gene="ectA"
                     /locus_tag="L3Q65_RS32335"
                     /old_locus_tag="L3Q65_32335"
     CDS             complement(6717..7211)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020664377.1"
                     /locus_tag="L3Q65_RS32335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L3Q65_32335"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_182896435.1"
                     /transl_table=11
                     /translation="MSGKHLIESPTKADGAALWRIARDSQKLDLNSPYAYLLWCKDFAE
                     TSVVARVDGEPVGFVIAYRRPSAPDVALVWQVAVDASQRGKGLAGQLLDSLYTRLVGEG
                     VRYLETTITPDNAASIRLFESFAKRWDADLEHRRLFEVADFPQGEAHEPEDLYRIGPLR
                     R"
     misc_feature    complement(7692..7710)
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 20.72"
                     /tool="antismash"
     misc_feature    7997..8011
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 18.85"
                     /tool="antismash"
     misc_feature    complement(8992..9006)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: strong, score: 19.46"
                     /tool="antismash"
     misc_feature    complement(9693..9711)
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 20.99"
                     /tool="antismash"
ORIGIN
        1 tcggactggc cgcggccggt gtcgccttcc tcccggattg gggtttcctc gcctggccgc
       61 tcgccggggt cggcctcgcc gggggaattg cgggaatcgt actttcctcc caagggaaaa
      121 tgcgcggact gggcgtcgca ctcaccggaa ccacgctttc cgcactggcc gccctgctgt
      181 gcggcgcgat gctgctgtac ccgtcgatgt tcggcaccgc gcgggcgggc gaactgcaca
      241 tcccgccgca accgggcgag aagcattcgg tcgacttcgt ggtgtcctcg gcgaacggcg
      301 cgacggtccg cttcggcacg ctgaacgacc agcgcaccgc gaccaccccg ccgagcaccg
      361 acgactggca cggacacgct tcctacgacg gcggcaggcc ggtcctctcg ctcaccgcgg
      421 acaccgccaa cgcgggcgtg accaaccaga tcagctgcca gatcctcgtc gacgggaaga
      481 agatcgccga gagcagcggc agttcgatcg cgctctgcac ctcgaacgtc gaatagagcc
      541 tggcggccgg ccggcctacc cccatctcgg ccggccaacc gccaccgaac cgctcctggc
      601 ggctcttcca ggtccgcgcg gtattcgccg cggtcccgtc gccggcttca cagcctgccc
      661 ctcccctagg gctccgccag cgtcgcggcc gaccctacgc gcgctgaccg cccgatggct
      721 aagaatccgc taagcggacg cgattattac cacggcgacc tgcgaaaaca cagctgaacg
      781 ttgtacaaga gccgggcgtg ggcgactgcc cgctcactcc cggccggatg ctttcttgcc
      841 cgctcggcgg cattccgcgg ccgacgtctt cagtcccggc acgagcggaa ggatccggcg
      901 ttccgggaat cagccgctac tgatctccca gatcagccgc ttccttccgc aagtcctcga
      961 cctgccgagg gttcatccgc tcgtacaacg ccaccgcccg ccggtactgc tccctcgcga
     1021 gctccagccg ttcctggccg cgcaggatcc gcgccagggt cacccgcaag atgccctcct
     1081 gcagttcgaa ctgccgcgcg accgccagcg acacggcctc cctcgcgtag cgttcagcgg
     1141 cctccaggtc gccgatgccc agatgcagtt ccgcgaggtt gttcagcgac accacggcat
     1201 agctgtcgtc gccgagttcg cggtctatct ccagcgacgc cagctgatgc gtgatcgctt
     1261 cggccggacg gcccgcgcgc ttctccgtct ccgcgcagtt gttgagcgcc tggccgcgca
     1321 gttcgagatc gcccgtgcgc tcggtctccg cgaccgcctc cagcagccgt tcgatcgcct
     1381 ccgaatagcg gccgagcagg gtcagcacgc tgccgaggtg gattttggcg acgatgagcg
     1441 agcggccgtc gagccgggtg gccaagccga ccgcgcgttc ggcgtcggcg atgctggcct
     1501 gcgaaagctc gaaggtgagc gcgatggcgc agcgggagat cagcatccag cactgcccga
     1561 gcggttcgtc cgcggcttcg gcggcggcga ggccgaggtc gaccagccgg atccattcct
     1621 cgtacagcgg gcagacgacc cggtaggtgt gcgcgacgcg ggcgagccgc cagacgtcgg
     1681 cgtagcggcc gtcggcgtgg gcggcgtcga gcacggccag caggttcggc cattccgcgg
     1741 cgaaccagtc ctgcgcctga gcgaaggttt ccagcggcgg cagcgcgtcc ggcggcacga
     1801 ggccggtgag gtcgagcggg tcgacgatgc gcagcatctt ccgccgcgcg gtgtccgagg
     1861 cggcctggtg gtagcggatc gcccggtcga gcaccgccgc gcgttcggct ggctccagtt
     1921 cggtttccgc cagctcccgc aggtacagcc ggaccaggtc gtgcggggcg aaggcgtcgc
     1981 gcgaagtctc ggcgatcagg ttgtgcgcgg ccagcgtgcg gagttcgcgg cgggcctgct
     2041 gcaccgggat ctgcgcgatg gccgccgtca ggtgcgggcc ggtgtgccgg gcgggcacgg
     2101 cgcccaggcg cagcagggtc cgggccagtc cgtccggcag gccgcggaag gagacgtcga
     2161 acgcggcccg gacgccgtcg tcggctccgg tgacatcgag tccggccagc cgggtgcgtt
     2221 cgtttcccag ctcgccgacg agatccttga gtgtccactg tggactcgcg gcgagcttgg
     2281 ccccggcgat ccgcagcgcg agcggcaggt agccgcacag ccgggccagc gcgtggttga
     2341 ggtcgaagtc ggcgggcccg gccaattcct cgatcacgct caccgcgtct tccggcgcga
     2401 gcgtgccgag cacgcgcagc ttcgccgcgt gggacaccgc cagcgcctcc agtcgcgacc
     2461 ggctggtcac caccgtcatc gaccgtgcgc ccggaggaag caacggacgc acctgttcgg
     2521 ccgaacgcgc gtcgtcgagc agcacgagca tccgccgccc ggcgatcagc gagcggtaca
     2581 gcgcgacgcg ttcgtgcacc tgctccggca cctcgtccgc gggcacgccc agcccgagca
     2641 ggaactgcga cagcagatcg cctggttcca gcggcggctg atgcggatcg aacccttgca
     2701 gcgccgcgta cagcaccccg tccgggaaca gccgggccgc gcggtgcgcc caccagatga
     2761 ccagcgcgct cttgccgacc ccggcggtgc ccgacacgat gaccgtgctg tcgccgcgtt
     2821 cagcgagccc gtcgagccat tccacctccg tctcgcgccc ggccagcgtc ggcagcgcgg
     2881 gcggcagctg agacggcgcg acggcctgct ccggaaccgc gctctcgtgc gcggcgagat
     2941 cgtcgttgag cacccgctcg tgcagcgagc gcagatccgc gccgggttcg acgccgagcg
     3001 agccgagggt ggcccgcgag acggtgcggt acagctcgag cgcttccccg cgccgcccgg
     3061 cgtggtagag cgcgcgcatc agctgtccgg cgatgcgctc ggcgagcggg tcggcgcgga
     3121 cgatcgggct cagctccacg atcagctcgt cgtagcggcc gaggtcgaga tccgcgttga
     3181 tccgggcgta gcggatcgcg cgccgcaact cctccaattc gggcgcccgc accgaatcgg
     3241 gcacgccgga cagcgtgggc ccctgccaca gcgcgagagc ttcggcgagc agcttcgagg
     3301 cgctctccgg cgattccagc gcggcctggt ccagcaacgc ccgcgcccgg tgcgcgtcga
     3361 tccggtccgg cgcgagggaa agctggtagc ccggcggacg ggtgctgatc tcggcgaccg
     3421 gatcggccat ctcccgcagc agcctgcgca actgcgacac gttgccgtgc acgatggtgc
     3481 gagcggtggc gggcggatcg tggccccaca gcgcgtcgat gatctcctcg agcggcacca
     3541 ccttgcccac gcgcagcgcg agcagcgcca gcagtccccg caccccgggg ccgccgacgg
     3601 gtaccgcacg gctgccgtcg agcagccgca ccgggccgag cagttcgacc cgcgcgccgc
     3661 caccggccat gctcgcgcgc acctcccagg ctcaccccca gccagacagg tcaacttacc
     3721 gtcagcggca gcccgcagaa accgctcttt cgtgaatttc gcccctgaac agacgagtcc
     3781 ggccccgcac catcagtccc gatctgatgg tgcggggccg gactcaatga tcgtcggccg
     3841 ggccgccagg gggcgcggtc cacccgtgca tcgcggccgt ccgcccgcgg ttcggggggc
     3901 tcaccccgag caagaacccg gaccgccgcg gacaaccatg cctgcgcaga cgagctgcga
     3961 ggtggcgaca acgtaacagg gcgcacattc ttcgttacga gcgacccgcc gataggcaaa
     4021 ggtaacgctg gtcaccgcgt gatcggagcc gagttgcggc ccgcgatgaa cgacggccgc
     4081 ggcgcgctcg cggcgaacgg ctcctgcaac gcgttctcca cgctgttgaa caccaggaag
     4141 atgttcgagc gcggatacgg cgtgatgttg ttcgacgatc cgtgcatgat gttcgagtcg
     4201 aaccacagcg ccgagcccgc cgaaccggtg aactgctcga tgccgcagtc cgcggcgagc
     4261 ttggtgatgt cgtcgttggt cggcaccccc acccgctggt ccttgagcga gctcttgtag
     4321 ttggcgtccg gggtctcgcc gacgcactgc acgaacgtgc gctgcgaacc cggcatgacc
     4381 atgagcccgc cgttgtacgg gtagttgtcg gtgagcgcga tcgagcagct caccgcccgc
     4441 ggcgacggca tgccgtcctc cgcgtgccag gtctcgaaat cggaatgcca gtagaagccg
     4501 gtgcccttga agcccggcat gtagttgatc cggctctggt ggatgtagac ctcggagccg
     4561 aggatctgcc gcgcccggtc gagcacccgg ggatcgcgca ccagctcggc gatcaggccg
     4621 gagatctcgt gcacgtcgaa gatcgaccgg acctcgccgg tccgcgcctc ggtgatcacg
     4681 cgttcgtcgt cgcgcagctt ctcgtccgac gacagccgga cgacctcctg ccagtacgtc
     4741 tggacctcac cgggcgagag cagcccctcg acgaccgtgt agcccttggc gtcgtggttc
     4801 gccagggtcg cggcgtcgat cgggccgtca gcctcggtac cccacaccgt ggggtgcacg
     4861 cgcggaaggt gctccggcgt accggtgatc cgggtcgggt aaccgtcttc gacccgggtg
     4921 tccatgatcg tcacagtggc tcgcctccag ttcttctggc gtttcttctt tctcggggtg
     4981 gtggtgggag cgacgcagtg tcaggagtct tcggtgacga gcgggtagac cccgttctcg
     5041 tcgtgcacct cgcggccggt gaccggcggg ttgaacacgc acacgcactt gatctcggtc
     5101 ttcgggcgga cctggtgctt gtcgtggtcg ttcagcaggt acagcgtgcc cggcttgagc
     5161 tggtgcacct caccggtggc gaggtcttcg atctcgccct cgccggaggt gatgaaaacg
     5221 gcctcgatgt ggttggcgta ccagaagtcg ttcaccgtgc ccgcgtacag cgtcgtctcg
     5281 tgcaccgaga agccgacgcc ctccttcgcg aggatgatgc gcttgctgcg ccagttcggg
     5341 gtcttgatgt cagcgtcggt gtcggtgatc tcgtcgagcg tgcggaccag caagtgaaga
     5401 gctccttctt gttcttcggg gtggttactt gaggacggcg gcgacggact cggtgatgat
     5461 cgcgagaccc tcgctcagtt cctcgtcggt gagggtaagc ggcggcagca gtttcatcac
     5521 ttcgccgtcc ggaccggagg tctccatcag caggccgcgt tcgaaggcgg ccttgcagac
     5581 cgcgcccgcc agctcgccgc tgccgaattc gagaccgcgc gccagaccgc ggcccttcgc
     5641 gacgaggttc gcctccgggt agctctccac caggtccttg aaggtgctcg cgatgcgctc
     5701 gcccttggcc ttggtcgact tctccagctc gtcgtcgctc cagtagacgc gcagcgcctc
     5761 ggtcgcggtg acgaacgccg ggctgatgcc gcggaaggtg ccgttgtgct cgcccggctc
     5821 ccagacgtcc aactccggct tgatcagcgt gagcgccatc gggaggccgt agccgccgat
     5881 cgacttcgac aggcacacga tgtccggcga gatgccggcg tcctcgaagc tgaagaacgg
     5941 gccggtgcgg ccgcagccca tctgcacgtc gtcgaggatg agcaggatgc cgtgccgctt
     6001 gcacaggtcg tcgaggccct tgagccattc caggcgcgcg gcgttgatgc cgccctcgcc
     6061 ctgcacgccc tccacgatca ccgcggcggg ctcgttcagg ccgctgccgg agtcttcgag
     6121 cagcttctcg aagtacatga agtccggcat cgcgccgtcg aagtacttgt catacggcat
     6181 cggcgtcgcg tgcaccagcg ggatgcccgc ggcaccgcgc ttcatcgagt tgccggtgac
     6241 cgacagcgcg ccgagcgtca tgccgtggaa cgcgttggtg aagttgatga ccgattcctt
     6301 gccggtcacc ttgcgcgcca gcttcagcgc ggcctccacg gcgttcgcgc cgccgggccc
     6361 ggggaagacg atcttgtagt ccagctcgcg cggcgcgagg atcttctccc ggaaggtctc
     6421 caggaagtcc cgcttcgcga cggtgtacat gtcgagcgcg tgcgtgacgc cgtcgcgctg
     6481 gatgtaatcg atcagcgcct tcttcaggtg cgggttgttg tgcccgtagt tcagcgcgcc
     6541 ggcaccggcg aagaagtcga ggtaggcctt gccgtcctcg ctgtagagcc tgctgccctg
     6601 cgcccggtcg aagacgaccg gccacccccg gctgtagctg cggacttctg attcgagctc
     6661 ttcgaagatg ctcataacga tttctttcgc tcctactcgg cgaaattgac gggttattag
     6721 cggcgcaacg gcccgatgcg gtaaagatcc tcgggttcgt gcgcctcccc ctgcggaaaa
     6781 tcagcgacct cgaacaaccg gcggtgttcc agatccgcgt cccagcgctt cgcgaacgac
     6841 tcgaagaggc ggatcgacgc cgcgttgtcc ggagtgatcg tggtttccag gtaccgcacg
     6901 ccctcgccga ccagacgcgt gtacaggctg tccagcaact gcccggccag gccttttccg
     6961 cgttgcgacg cgtcgacggc gacctgccac acgagcgcga cgtccggtgc ggacggcctt
     7021 cggtaagcga tcacgaatcc gactggctca ccgtcgacgc gggcgacgac cgaggtctcc
     7081 gcgaaatcct tgcaccacaa caggtacgca tacggcgagt tgagatcaag cttctgcgaa
     7141 tcgcgtgcga ttcgccagag agcggcacca tcggccttgg tcggggattc gatcaagtgc
     7201 tttccggaca tactttggaa acgtaacaga gaattttcgc gccgccagcg atccatgcca
     7261 cgtccaccgc cctcaccagg gacgacaggg ataccccggt gaggaggacc acaaacatgt
     7321 ttaacgaggc gaagttctgg gcatgcgtgc gcctgggccg cacaccatcg gtatgcgacc
     7381 tgtgacccca gaccatgggc cttccggggc gctaccgcaa ctccagcgcc gaaaaatggg
     7441 gtgctacctg gggttcagcg tcgactgtcc gcacgcgacc gtcgccgctt ccgtccgcgc
     7501 cgcccgagcc cggacagcgg cgtccagacc gcgggcagca ggagcagcgc gacgacggcg
     7561 agccaccaca gcggagcctg gccgcgccgc caaccgtcca caacggtgcc gagccagccg
     7621 aacaacggct gcgcgagggg cggcaggaag gccgcgaaca gcaacgcggc tatcaccagc
     7681 acacccagca cggtgagcac cattcgccac gtgcgcctca aggtcagcag gctcaccacg
     7741 aggaacacga gtgcgccgcc cgccaccggc aggccgagcc attgcaggac ggacccgcct
     7801 tcgccgctga gcacgaaccc ggcgatcgcg gtgatggcgg cggccagcag gttccagggg
     7861 cggcccagcc gcgtcgcgcc ttgggtgacc caccacgaac tgcggctgct gagctgcgcg
     7921 aatttcgcca gcggctggag ggttttcggg acccgggtgg cagtggacgc ggcgttgagc
     7981 ccggcggctc cggctttgac aatcgttttg gtgagcagtg tactgccgag ttcgccttcg
     8041 aagcgttcag cggagacttg gcaggcttgg aaagcgcgct gagccgcgtc ctgctgaccg
     8101 gaattttccg gcggggcggc ggctttccgg gattcttgtt cgagcggccg ggaaaccatc
     8161 agcttccgga aactctccga cgcttttccg ttgccctcct tcgcatcctg ctcggcctgc
     8221 tgcgcgagca ctggcaattc ctcccgcgcg atcgtcagct gccgcgactt ggccagcacc
     8281 atcgacgtca ccggaagact ggtcgggatg tccttgccga agtcgaccgg cgacagattc
     8341 tcgtccaggc cgaggaaagc cagttcgtcg cccagctggg cgagcagccc gggcaggtcc
     8401 gagggcatcc cgttgtccgc gggcgaaggc ggctcccagc cgatctcgcg aaacgtcgcc
     8461 cttacctgct cccggaattc ctcagggccc acgatgtcgc gcagcacccg caggcgtttc
     8521 gggtcgagca ggcactgcat cagccagccc gcgccgtcga tccggcccca catccagtcg
     8581 ttcgcgcgcc acgacgcttt gtagaagccg ccgaagttgc tcgcctgcgt accggtcagc
     8641 ttgtcccggc cgcgggggcg cgccaggtcg agcagggttc ggctgtccgc gctcatctgc
     8701 accaggtcga cgcgctggtc caccgccggc gcttcggcca gcagcccccg cgttgccatg
     8761 tgcagcaaca ggaatcgcgc catcacgaat ccgtccgcga cgtccgcgtt gtcaaggccg
     8821 aaccagtcca gcagcgtcgt caccgtgtcg gcggcgtccg cggaccactg gttttccttg
     8881 cccgacaacg cttgcagcac cggagcggcc gaccgcagcg cgccggtcag ctccggccac
     8941 gcctcgccga cggcgtccga cgccccgaga tccgcccatt cggcggcgag ctggccgatc
     9001 cagtccggca tcgaggtgcc tggcggcggc ggttcgtgcc gggccaccca cgcgggcaac
     9061 cgcacgtcgc gagccgcgct ccgccgggcg gcgtgcatct tcgcgcgggc ctcgttgagc
     9121 tgggtcgcct ggtcgagatc cgggcccatc cggaagcccg cgttgatcag ctggatgccg
     9181 gtggcgaccg cgtcttccag cgttgaagtg cggtagcgga ccatttcgcc gaccgcgacc
     9241 gattcgccgg gcaacgcggc cgggatcccg cggcgcaatc cgtcggcagc gatgtcctgc
     9301 agcagcgccg aattcccggc cgcccactta ttgtcctcat cggacagttc gtagcggcgg
     9361 gtgaccgcgc gaaccagctc ggccgcgtcg agcgcgaccc ggcggtcgag gtacgcccgg
     9421 tgcacccggt cgtccaccag ctgcccgccg cgcaggccga gcgacgcgag cgcgacccgg
     9481 gtgtccctgg tgcgcgtgac ggcggagttg tgcttgctca attcctccag ctcgacgctg
     9541 atggtctggc tcatgatcgt ggaggcgacc ttcgccagcg cggtgccgag catcggcgga
     9601 tccgccggat cgcaggtcat cggcttggct tcgccgtcga ccgtcgggac gacgtacagc
     9661 agcagccgtc gcacatccgt cgacgaagag cgttcgaaaa tctctcgcaa cgccggtcgc
     9721 aagggcttgt tcaccagcac gccgccgtcg gcgagccaat gcgaacgcac cagctcggtg
     9781 taatcggtca tgtccggatg cagccggtcc gccgcctcgg ggccgatcgg catgcgcgac
     9841 agttcgaagg caccggggaa ggacgcgctc gaccgggcgg cgagcgcgag cgggccgtgc
     9901 acctggttat tccacaatgg accgtcgaag cggaacagca tgcgatgttc ggtgtcccgc
     9961 acccggtttc cgagcgcgtc gtcgtactgg gtggtctcgc cgtcgagcat ggtgccggtg
    10021 agcaacacgg tcacatccgg cggctcagtg ggcacgacgc cggacccggt gatgtcgtgc
    10081 atcgcctgga cgagtccgcc cagcagcacc ttgtctccgt tcagcaccga acgcggccgc
    10141 gtctcgccgg ggtcgcggat cagattctcc agcgacccgg cggagaacca ggtgtcgcgc
    10201 aagatcgcgg gcgtcgaccg gtacgcctcc gccagtccga gacacgcggc gttgatgccg
    10261 cccgcgctcg ttccggtgag cacgtccagg cgcaccgaag cgtgcaaaag gtcgagcagt
    10321 ttccggtagc cgttactttc gtccggcgtg cgcgaagcgt gcagcaacgc cgacgtctcg
    10381 gtagcgactc ccc
//
