LOCUS       NZ_CP091381            10798 bp    DNA     circular CON 18-AUG-2024
DEFINITION  Amycolatopsis sp. FU40 chromosome, complete genome.
ACCESSION   NZ_CP091381
VERSION     NZ_CP091381.1
KEYWORDS    RefSeq.
SOURCE      Amycolatopsis sp. FU40
  ORGANISM  Amycolatopsis sp. FU40
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Amycolatopsis.
REFERENCE   1  (bases 1 to 9944196)
  AUTHORS   Reisman,B.J. and Bachmann,B.O.
  TITLE     Biosynthesis of Apoptolidin Family Glycomacrolides
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9944196)
  AUTHORS   Reisman,B.J. and Bachmann,B.O.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2022) Chemistry, Vanderbilt University, 1234
            Stevenson Center Drive; Room 7330, Nashville, TN 37235, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 23-JAN-2022
            Assembly Method                   :: Flye v. 2.9
            Assembly Name                     :: NFU40_flye_2.9
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 47.0x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_021903635.1-RS_2024_08_18
            Annotation Date                   :: 08/18/2024 04:30:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,491
            CDSs (total)                      :: 9,417
            Genes (coding)                    :: 9,360
            CDSs (with protein)               :: 9,360
            Genes (RNA)                       :: 74
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 7 of 57
            Pseudo Genes (incomplete)         :: 50 of 57
            Pseudo Genes (internal stop)      :: 5 of 57
            Pseudo Genes (multiple problems)  :: 5 of 57
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:13:09
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6396412
            Orig. end                         :: 6407210
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP091381.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Brian O. Bachmann,
            Vanderbilt University.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10798
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="30"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10798
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10798
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5798](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5798)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            688..921
                     /locus_tag="L3Q65_RS29875"
                     /old_locus_tag="L3Q65_29875"
     CDS             688..921
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009083248.1"
                     /locus_tag="L3Q65_RS29875"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L3Q65_29875"
                     /product="hypothetical protein"
                     /protein_id="WP_182889886.1"
                     /transl_table=11
                     /translation="MTVRSRVTDEVTTWLTGEFAGRVPAEAVKVVVRAAGRDLDGRVVP
                     DEHGDLLYRVARARLVRMLSAPEEPRIPRSRG"
     gene            complement(1026..1163)
                     /locus_tag="L3Q65_RS29880"
                     /old_locus_tag="L3Q65_29880"
     CDS             complement(1026..1163)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="L3Q65_RS29880"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="L3Q65_29880"
                     /product="hypothetical protein"
                     /protein_id="WP_237597229.1"
                     /transl_table=11
                     /translation="MHWNRWQSGADALELPDDDVPPPPAQVWWPPERSHAPLSSRMPGS
                     "
     gene            complement(1519..2367)
                     /locus_tag="L3Q65_RS29885"
                     /old_locus_tag="L3Q65_29885"
     CDS             complement(1519..2367)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009083249.1"
                     /locus_tag="L3Q65_RS29885"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L3Q65_29885"
                     /product="ion transporter"
                     /protein_id="WP_182889885.1"
                     /transl_table=11
                     /translation="MTTGRPAQAELNSGILPGNVRADDWIMLALAVVSTGMLAWLLISP
                     PAPEISHWFFIADCVICGIFFLELLHRWRKQAWRAAFLARTWYEIFAMIPVAHPAFTAN
                     HHVLGVILLIIRLGRAADRAVGEQFTYRLVDKLSEPIVRAIKKPVTIAVLDEVVKVLET
                     GNYPENLAKSLSENKTELRAIITEKISEDPQLGKLRRLPFHDEIVRATVDTSFRVLLEV
                     LLDPRIDDFFSSVVRDNREQIRRAVALGLADVDDSPDEQLLRTRTQRTAAHEYDAHHPR
                     R"
     gene            3159..4364
                     /locus_tag="L3Q65_RS29890"
                     /old_locus_tag="L3Q65_29890"
     CDS             3159..4364
                     /GO_function="GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009083250.1"
                     /locus_tag="L3Q65_RS29890"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L3Q65_29890"
                     /product="glycosyl hydrolase family 8"
                     /protein_id="WP_182889884.1"
                     /transl_table=11
                     /translation="MPASRKVALFAAASVSLTAALAGAGPAAAASAARPFPAHVTYQTG
                     VRPSVSQAEQDAAVKKQYDSWKKTYLRQACGGYLVWATDDAPNKGTVSEAQGYGMNIVP
                     LMAGYDDQAKTIFDGMWKFVQDHRDSKGLFQWVIDGKTCKYADDGTPDSATDGDLDVGY
                     GLILADKQWGGYSAAAKDWLAKIYKYDVASDGHLKIADDAGNDDTRPSDFMLDHLRAFA
                     KYDTAHDWNKVVTRTEQLITQFTAKYSPDANLLSDFVVGANTNSPKPAPADHLENQPDN
                     IVGYNSVRVPWHMGTDALLYGGKVATEVAKKESASYKSHSGGNPAKIYPHTKLDGTPTK
                     TSDQSEVAANPVGVAAMVAGDQAWTDSLWKYLGSNPYDDTYYGETIKLLVYLVMSGNYW
                     AP"
     gene            complement(4389..4916)
                     /locus_tag="L3Q65_RS29895"
                     /old_locus_tag="L3Q65_29895"
     CDS             complement(4389..4916)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009083251.1"
                     /locus_tag="L3Q65_RS29895"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L3Q65_29895"
                     /product="M15 family metallopeptidase"
                     /protein_id="WP_182889883.1"
                     /transl_table=11
                     /translation="MLRNKILLMLPAFLVAGCSGLPVLDGDPDHDPAQGGAPEGVTLTP
                     FDTSYPAVGNLDPALRKALQEAATDARQRKVDIHIASGWRTKEYQQKLLDGGIEKYGSL
                     EEARKFVNTPEKSTHVSGKAVDVAPTRAADWLIQHGEKYGLCQVYANEMWHFELLTAPG
                     GTCPPARTDAAG"
     misc_feature    4970..4986
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 17.13"
                     /tool="antismash"
     gene            complement(5001..5798)
                     /locus_tag="L3Q65_RS29900"
                     /old_locus_tag="L3Q65_29900"
     CDS             complement(5001..5798)
                     /GO_component="GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Linocin_M18"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009083253.1"
                     /locus_tag="L3Q65_RS29900"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L3Q65_29900"
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="WP_182889882.1"
                     /sec_met_domain="Linocin_M18 (E-value: 1.1e-79, bitscore:
                     266.2, seeds: 61, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNNLHRELAPISAAAWADLEEEAKRTFAEFAAARRVVDVVVTGDP
                     TLAAVGTGRRERVGGKFDGVATSLRTAQRVVELRVPFTVSREEVDSVEWGAKDADWQPV
                     KDAAQQIAYAEDRIVLDGFAEAGITGIRPASSLSPIALPPEAPGYPKAIGDAIAELRDA
                     GVAGPYTVLLSDDEFTAAYEAANHGYPVLEHLKQIVDGELIRTPAINGACVLSTRGGDY
                     ELHFGQDLSIGYSSHDASTVELYFYETLTFLVNTAEAGIGLTA"
     gene            complement(5795..6826)
                     /locus_tag="L3Q65_RS29905"
                     /old_locus_tag="L3Q65_29905"
     CDS             complement(5795..6826)
                     /EC_number="1.11.1.-"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009083254.1"
                     /locus_tag="L3Q65_RS29905"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L3Q65_29905"
                     /product="Dyp-type peroxidase"
                     /protein_id="WP_237597230.1"
                     /transl_table=11
                     /translation="MQAGDLEPPEPQAVSAPLTRAAIFLMVQVDAGAEQTAKDLLADLA
                     GLQRAVGFRDAGVGLTCVAGISSAAWDRIYGGPRPAELHELPVFSGEKHTSVVTAGADL
                     LFHLRAERLDLCFELESLIMDRLRGSVKVLDEVQGFRYFDARDVLGFVDGTENPTGNGV
                     HQAVLVDDDEPFNRGSYVVVQKYLHDMAAWNALTTEQQELVIGRRKLSDVEMPDEEKPA
                     DAHIALNVITDDEGNELDILRDNMPFGRPGHDEFGTYFIGYTKSPAVIEKMLENMFVGS
                     PPGTYDRILDFSTPHTGALFYVPTADFLEDPPDAPPEPEPPAAATPAGTSLGIGSLRRS
                     IRS"
     gene            complement(6934..7548)
                     /locus_tag="L3Q65_RS29910"
                     /old_locus_tag="L3Q65_29910"
     CDS             complement(6934..7548)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020664854.1"
                     /locus_tag="L3Q65_RS29910"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L3Q65_29910"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_043828030.1"
                     /transl_table=11
                     /translation="MTQAKERISRRAVDKFAQRREQLAESALQALAELGYARTSLREIA
                     QKSDFSHGVLHYYFADKVELLTYAVRKFEEVCVTRYDDVVAEAGSAEELKHGFGAAMAG
                     TLRTDAAMHRLWYDLRNQSLFEESFRGDVLDIDEQRQDMVWRVVRRYAEFTGRPAQVSP
                     EVAYAAFDGLFQRALLQQLAGQDAAADGLEADVAALLTHFV"
     gene            7627..8475
                     /locus_tag="L3Q65_RS29915"
                     /old_locus_tag="L3Q65_29915"
     CDS             7627..8475
                     /EC_number="1.1.1.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /GO_function="GO:0070403 - NAD+ binding [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_KR (24-189). E-value: 2.4e-14.
                     Score: 45.6. Matches aSDomain:
                     nrpspksdomains_L3Q65_RS29915_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short_C2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005166150.1"
                     /locus_tag="L3Q65_RS29915"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L3Q65_29915"
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_237597231.1"
                     /sec_met_domain="adh_short (E-value: 1.5e-32, bitscore:
                     112.1, seeds: 230, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short_C2 (E-value: 7.7e-34, bitscore:
                     116.8, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTYTALAARLKEEMSMGSYDLTGRTALVTGGAQGLGQGMAEALAK
                     AGAAVVIADVQEDLGRQTADAIKQSGASSGFVRLDVTDDDNWARAVEAVVGEHGGLDIV
                     VNNAGVEITGLVADLDPADVRKMLEVNVLGTSLGVKHAFRAMRPGGVAGRGGAVVNIAS
                     VAATIAFPGIAGYSATKSAVDRLTRVAAMEAGKLGYGVRVNCVYPGLVPTQMGNQLAAD
                     VVDVGLFPSIEDAIGAVVAQTPAGRLGEVADMADAVVFLASDAARFITGAGLPVDGGMG
                     M"
     aSDomain        7699..8193
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_L3Q65_RS29915_PKS_KR.1"
                     /evalue="2.40E-14"
                     /label="L3Q65_RS29915_PKS_KR.1"
                     /locus_tag="L3Q65_RS29915"
                     /protein_end="189"
                     /protein_start="24"
                     /score="45.6"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="TALVTGGAQGLGQGMAEALAKAGAAVVIADVQEDLGRQTADAIKQ
                     SGASSGFVRLDVTDDDNWARAVEAVVGEHGGLDIVVNNAGVEITGLVADLDPADVRKML
                     EVNVLGTSLGVKHAFRAMRPGGVAGRGGAVVNIASVAATIAFPGIAGYSATKSAVDRLT
                     RV"
     CDS_motif       7705..7842
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_L3Q65_RS29915_0001"
                     /evalue="2.00E-05"
                     /label="PKSI-KR_m1"
                     /locus_tag="L3Q65_RS29915"
                     /protein_end="72"
                     /protein_start="26"
                     /score="17.2"
                     /tool="antismash"
                     /translation="LVTGGAQGLGQGMAEALAKAGAAVVIADVQEDLGRQTADAIKQSG
                     A"
     gene            8519..9640
                     /locus_tag="L3Q65_RS29920"
                     /old_locus_tag="L3Q65_29920"
     CDS             8519..9640
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016333717.1"
                     /locus_tag="L3Q65_RS29920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L3Q65_29920"
                     /product="DUF5938 domain-containing protein"
                     /protein_id="WP_182889880.1"
                     /transl_table=11
                     /translation="MTKPVVVYGASGYTGRLICEYLREYNVAFTAAGRDKARVQAALDH
                     IPGLDTVDHEVVEVSHETGPLTELFRDASVVCNTVGPFSEYGHEVVQACLESGTHYLDT
                     TGEQDWLISAEERYGADMAAKGLLLSPGIAQMYTTGEIAANLCLETPGLDTLDILVFWK
                     GYPTVASTQTILVNAALSAAYYLEQNEYAPWPADGGLQDVAVPGHHEIGLALPWGGTSH
                     PVWFKNDPRVANVKAVGGVFDRALMQGVPQIVAAVLEQVKDLPLEEKLKVLHEQAAAVR
                     DEMPPRENQRINTSLDSVHASGPLGRAHCVIHGTCNYKQTGLMQAYAAYSLLQQPPRRA
                     GFASACQAFGHRELLGVLRSFGLVSNPVLTVQS"
     misc_feature    complement(10069..10083)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.8"
                     /tool="antismash"
     misc_feature    10468..10483
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 20.34"
                     /tool="antismash"
     misc_feature    complement(10577..10592)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 17.51"
                     /tool="antismash"
     misc_feature    10674..10689
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 22.5"
                     /tool="antismash"
ORIGIN
        1 cgtggtgcgc gacggcgacg aggcggtgct cggcaagccg atcgactggc tgggcgtgaa
       61 ctactaccgc ggctaccggg tggccggttc ggcgcggccg ggaagcacgc cggcgggccg
      121 ggaatggccc ggagccgacc acgtgcactt cgtcccggac ccggccgcgc cgcgtacgga
      181 ttccggctgg gaggtgcgcc ccggcgggct gaccgaatcg ctgctgcaag tgcatcggga
      241 ctatccgccg gtgccgttgt acgtcacgga gaacggcgcg gcgtatccgg acctcctgcg
      301 cgacggcgag gtttccgacg acgaccgcat cgccttcctc gacgcgcatc tgcgggccgc
      361 acacgacgcg ctggctcacg gagttgatct ccgggggtac ttctactggt cgctgctgga
      421 caatttcgaa tgggccgaag ggtacgcgaa gcgcttcggt ttggtgcacg tcgattaccg
      481 cactcagcag cgcacgccga agcgcagcgc acggtggtac gcggaggtga tcgcgcgcaa
      541 tgcgctgagg tgacctactc ccgggtttat ccgcaccgcc gggaatgact ttctcttccg
      601 gtttcgcgtc acgaggtccg gacgcggccg tcggaccagt gccggggccg ccccgaacgg
      661 ccccgcgagc ggagactgga gtgtccgatg acggtgcgga gccgggtgac cgacgaggtc
      721 accacgtggc tgaccgggga attcgcgggc cgggtgcccg ccgaggcggt caaggtggtg
      781 gtgcgggcgg ccggacggga cctcgacggc agggtcgtcc cggacgagca cggcgacctg
      841 ctctaccgag tggcgcgggc ccggttggtg cggatgctct ccgcaccgga agagccgcgc
      901 atcccgcgtt cgcgcgggta agccgagatt gtgcccggac ccgcacgggt ccgggcacaa
      961 gacttctccg gtggcgtgcg gtaaccaggc gcgcgccgac cgtgtccgac gcgtgccgag
     1021 agccgctacg agcccggcat cctgctgctc agcggcgcgt gcgaacgctc cggcggccac
     1081 cagacctggg cgggcggcgg gggaacgtcg tcgtccggaa gctcgagtgc gtccgcgccg
     1141 ctctgccagc ggttccagtg cacggaacac aatccgcgcc acaaggaatc ctccgcacag
     1201 tcccgccggc gacacctgcg tacctgctgc ccgctatcaa tcacgtccac gactccaaac
     1261 cgcgctcaac ctccccgccg gctccagtct gcctcggacc cggcgggaac gccccgtact
     1321 cgcgccaaca caacccttgt gcagcaacgg aacgagagat ctcaccctac tccagccgcc
     1381 ggtgcgcgca ccgcgcgacc acagtgcact ctttcagcgg ttgacgcgtt caccgcccgg
     1441 cgtcagcggt gaatctcctg tgccgcaacg gagagtgcgc acccgacggc ataccgtaga
     1501 tatgtccaca atggatgatc aacgccgcgg atggtgagcg tcgtactcgt gcgcggcggt
     1561 gcgctgggta cgggtgcgca ggagctgctc gtccggggag tcgtccacat cggccagtcc
     1621 gagcgcgacc gcgcggcgga tctgctcgcg gttgtcccgc acgacggagg agaagaagtc
     1681 gtcgatgcgc ggatccagaa ggacttccag caggacccgg aaggaggtgt cgacggtggc
     1741 gcggacgatc tcgtcgtgga acggcagcct gcgcagtttg ccgagctgcg ggtcttcgga
     1801 gatcttttcg gtgatgatgg cgcgcagttc ggtcttgttt tcgctgaggg atttggcgag
     1861 gttctccggg tagttcccgg tttcgaggac cttgaccact tcgtcgagga cggcgatggt
     1921 caccggcttt ttgatggcgc ggactatggg ttcggagagt ttgtccacca ggcggtaggt
     1981 gaactgctcc cccacggcac ggtcggcggc cctgcccagg cggatgatca ggagaattac
     2041 gccgagcacg tggtggttcg cggtgaaggc cggatgcgcg acagggatca tcgcgaagat
     2101 ttcgtaccag gtgcgggcca ggaacgcggc acgccaggct tgtttgcgcc agcggtggag
     2161 gagttccagg aagaagatgc cgcagatgac gcagtccgcg atgaagaacc agtggctgat
     2221 ttctggtgcc ggcgggctga tgaggagcca ggccagcatg ccggtggaga cgaccgccag
     2281 tgcgagcatg atccagtcgt cggcacggac gttgccgggg aggatgccgc tgttgagttc
     2341 ggcttgggcc ggacggccgg tggtcatgct ggctccctgg tggtggaggt gtggccgggg
     2401 gtgatgatcc catgggagcg ccggcggggc tgggaccgaa ggggcgtttt ggacggctcg
     2461 tgcttcgcac atcgccggcg agagttgcgg tgggccaccc cgaagtttga ttgtgctgac
     2521 ggttcggggg gtgcttgtca aggcgggaaa gatgccttga caagcacccc cgaaccgcag
     2581 ggcggcttcg tatcggggtt gggggagggc tgggtgcctc gatgggtggg tgcgctgacc
     2641 gcggctttgg cgggcggggg tttggtgctc aggcaggagg cggggttggt gcacaggctg
     2701 cggtttggcg ggcggagatt tggtgcgcag gctgcgggtt cgcggtgggc acgcggcttg
     2761 gatgctcggc cgggtcgggg cttgcggccg ctgcccggtg gggacgagta gcgccccaat
     2821 gtggcattgg gtgcgccaga cgcaccgaac gccacattgg gtgcgtcgca tgcaccgaac
     2881 gccacattgg ggcacatccc ggcgaccgtg gtgaggcagg ggccgtgaag ggagaccttg
     2941 agggaatcca cgtccgtgca gggcccctca cggaacgggc gagtgccgcg aggggaaccc
     3001 cgagggacgc agagtccttg aggggccctc acggaacgct tcggaaccgg ttcacctcgg
     3061 agttcgcagg gcggacatct tgacaccaaa cacccggtac agcaggcttt tctcacgccg
     3121 cctagttagg aaagtttact aacaaggaga ccccgaaaat gcccgcttcc cggaaggtgg
     3181 cgttgttcgc cgccgcttcc gtttcgctta ccgctgcgct ggccggtgct gggcccgctg
     3241 ccgcggcttc tgctgcgcgg ccgtttcccg cgcacgtcac ctatcagacc ggcgtccggc
     3301 cctcggtcag tcaggccgag caggacgcag cggtcaagaa gcagtacgac tcctggaaga
     3361 agacctacct ccgccaggct tgcggcggtt acctcgtctg ggcgaccgac gacgcgccca
     3421 acaagggcac ggtctccgag gctcagggat acgggatgaa catcgtcccg ttgatggcgg
     3481 gctacgacga ccaggccaag acgattttcg acgggatgtg gaagttcgtc caggaccacc
     3541 gggacagcaa ggggctcttc cagtgggtga tcgacggcaa gacctgcaag tacgccgacg
     3601 acggcacgcc tgactcggcc accgacggcg acctcgacgt cggctacggg ctgattctcg
     3661 cggacaagca gtggggcggc tacagcgccg ccgccaagga ctggcttgcc aagatctaca
     3721 agtacgacgt cgcgtccgac gggcatctca agatcgccga cgacgcgggc aacgacgaca
     3781 cccggccgtc cgacttcatg cttgaccacc tgcgcgcctt cgcgaagtac gacaccgcgc
     3841 acgactggaa caaggtcgtc acccggaccg agcagctgat cactcagttc accgccaagt
     3901 actcgccgga cgcgaatctg ctgtccgact tcgtggtcgg ggcgaacacc aacagcccga
     3961 agccggcgcc cgcggatcac ctggagaacc agccggacaa catcgtcggc tacaactcag
     4021 ttcgcgtgcc ttggcatatg ggcaccgacg cgctgctgta cggcggcaag gtcgcgaccg
     4081 aggtcgccaa gaaggaatcg gccagctaca agagccactc cggcggcaac ccggccaaga
     4141 tctatccgca caccaaactg gacgggacgc cgaccaagac cagcgatcag tccgaggtcg
     4201 cggccaaccc ggtcggggtc gcggcgatgg tcgcgggcga tcaggcgtgg accgattcgc
     4261 tgtggaagta cctgggaagc aacccatacg acgacaccta ctacggcgag accatcaagc
     4321 tgctcgtcta cctggtgatg tccggcaact actgggcccc gtgaccccgg acctgcccgc
     4381 gcggacggct aaccggccgc gtccgtgcgg gcaggcgggc acgtaccgcc gggggcggtc
     4441 agcagttcga agtgccacat ctcgttcgcg tacacctggc acaggccgta tttctcgccg
     4501 tgctggatga gccagtcggc ggctcgggtg ggggcgacgt cgacggcctt gccggacacg
     4561 tgcgtcgatt tttccggcgt gttcacgaac ttccgcgcct cttccaggct gccgtacttt
     4621 tcgatgccgc cgtcgagcag cttctgctgg tactccttcg tccgccaacc tgacgcgatg
     4681 tgaatgtcca ctttgcgctg acgggcgtcg gtcgccgcct cctgcaacgc ttttcgcaat
     4741 gccggatcga gattcccgac ggcggggtag ctcgtgtcga agggcgtgag cgtgacgcct
     4801 tccggggcgc cgccttgagc cgggtcgtgg tcgggatcgc cgtcgagcac cgggaggccg
     4861 gagcacccgg cgacgaggaa cgcgggcagc atgagcagga tcttgttccg cagcacgccg
     4921 caactatcgc gagcgggtat ccccggaagg tccggcgaat gtccgcgccg cgtaacaaga
     4981 ccgcatgctc aggtaacgcc tcaggcggtc agcccgatcc ccgcttcggc ggtgttgacc
     5041 aggaaggtca acgtctcgta gaagtacagc tccacagtgg acgcgtcgtg gctggagtat
     5101 ccgatggaca gatcctgtcc gaagtggagc tcgtagtcgc cgccgcgggt agacaggacg
     5161 caggcgccgt tgatcgcggg cgtgcggatg agttcgccgt ccacgatctg cttcaggtgt
     5221 tccagcaccg gataaccgtg gttggccgcc tcgtacgcgg cggtgaactc gtcgtcgctg
     5281 agcaggacgg tgtacgggcc ggcgacgccc gcgtcacgca gttcggcgat cgcgtctccg
     5341 atcgccttcg ggtagcccgg agcctccggc ggaagcgcga tcggcgacag cgacgaggcc
     5401 ggacgaatcc cggtgatgcc cgcctcggcg aaaccgtcga gcacgatccg gtcctcggcg
     5461 tacgcgatct gctgcgcggc atccttcacc ggctgccagt ccgcgtcctt cgcgccccac
     5521 tcgaccgaat cgacttcctc gcgcgacacc gtgaacggca cccgcagttc cacgactcgc
     5581 tgcgccgtgc gcaggctcgt cgcgacgccg tcgaacttgc cgccgacgcg ctcgcggcgc
     5641 ccggtgccaa ccgcggcgag tgtcgggtcg ccggtgacga cgacgtcgac cacccggcgc
     5701 gccgcggcga actcggcgaa ggtgcgcttc gcttcctctt ccagatccgc ccacgcggcg
     5761 gcggagatcg gggcgagttc gcgatgcagg ttgttcatga ccgaatgctc cttcgcaggc
     5821 ttccgatgcc cagcgacgtc ccggcgggcg tcgcggcagc cggcggttcc ggctcgggcg
     5881 gggcgtccgg cggatcttcg aggaagtccg cggtcggcac gtagaacagt gcgccggtgt
     5941 gcggcgtgga gaagtcgaga atccggtcgt aggtgcccgg cggcgacccg acgaacatgt
     6001 tctccagcat tttctcgatc accgccggag atttggtgta gccgatgaaa taggtgccga
     6061 actcgtcgtg ccctggacgg ccgaagggca tgttgtcgcg caggatgtcg agttcgttgc
     6121 cctcgtcgtc ggtgatcacg ttgagcgcga tgtgcgcgtc ggcgggcttt tcctcgtccg
     6181 gcatctccac atcggacagt ttgcgccgtc cgatcaccag ctcctgctgt tcggtggtga
     6241 gcgcgttcca cgccgccatg tcgtgcaggt acttctgcac gaccacgtag ctgccccggt
     6301 tgaagggttc gtcgtcgtcc accagcaccg cctggtggac cccgttgccg gtcggattct
     6361 ccgtgccgtc gacgaagccg agcacgtcgc gcgcatcgaa gtaccggaag ccctgcacct
     6421 cgtcgagtac cttcaccgaa ccgcgcagcc ggtccatgat cagcgattcc agctcgaagc
     6481 agaggtcgag ccgctcggca cggaggtgga acagcaggtc ggccccggcg gtgacgacgc
     6541 tcgtgtgctt ctcgccggag aaaaccggca gctcgtgcag ttccgcgggc cgcggaccgc
     6601 cgtagatccg gtcccatgcc gccgacgaga tcccggcgac acaggtcagc ccgaccccgg
     6661 cgtcgcggaa accgaccgcg cgctgcaacc cggcgaggtc ggcgaggagg tccttcgcgg
     6721 tctgttcggc accggcgtcg acctggacca tcaggaagat cgccgccctg gtcaacggcg
     6781 ccgaaacggc ctgcggctca ggcggttcaa ggtcccccgc ttgcacgctc gcgcacccct
     6841 tctcgcccgg tgaccgtccg accgcatcct aaccacggac gggcgagtgc acccggcatc
     6901 accactcggg cggggtaacc ggcggggtaa ccgtcagacg aagtgcgtga gcagcgccgc
     6961 gacatcggct tccaatccgt ccgcggcggc gtcctgaccg gcgagttgct gcagcagagc
     7021 ccgctggaac aagccgtcga aggcggcgta cgcgacctcc ggcgacacct gggccggccg
     7081 gccggtgaac tcggcgtagc gccgcacgac gcgccacacc atgtcctgac gctgttcgtc
     7141 gatgtcgagc acatcgccgc ggaaggactc ctcgaacagg ctctggttgc gcaggtcgta
     7201 ccagaggcgg tgcattgccg cgtcggtccg cagcgtgccg gccatcgccg cgccgaagcc
     7261 gtgtttcagc tcctcggccg agcccgcctc cgccaccacg tcgtcgtagc gagtcacgca
     7321 gacctcttcg aacttgcgaa ccgcgtaggt gagcagctcg accttgtccg cgaagtagta
     7381 gtgcaggacg ccgtgcgaga agtcggattt ctgggcgatt tcgcgcaggc tcgtgcgggc
     7441 atagcccagc tcggccagcg cctgcagcgc ggactcggcc agctgctcgc ggcgctgggc
     7501 gaacttgtcc acggcgcggc gggagatgcg ctccttcgcc tgtgtcacac ccggcagtct
     7561 agcgcgccac cgattcttga cgagtgtcca agaaaatctt gacgactgtc caaagttggc
     7621 tacggtgtga cctacaccgc actcgcggca cgtctcaagg aggagatgtc gatgggttcg
     7681 tacgacctga ccgggcgcac cgcgctggtc accggcggcg cacagggcct gggccagggc
     7741 atggccgagg cgctcgcgaa ggcaggcgcg gccgtggtca tcgccgacgt ccaggaggac
     7801 ctcggccgtc agaccgccga cgcgatcaag cagagcgggg ccagcagcgg attcgtccgg
     7861 ctggacgtga ccgacgacga caactgggcg cgggcggtcg aggccgtggt cggcgagcac
     7921 ggcgggctcg acatcgtcgt gaacaacgcg ggcgtggaga tcaccggtct cgtcgccgac
     7981 ctcgacccgg ccgacgtgcg caagatgctc gaggtcaacg tgctcggcac ctcgctcggc
     8041 gtgaagcacg cgttccgcgc catgcgtcct ggcggcgtcg ccggacgcgg cggcgcggtc
     8101 gtgaacatcg cctcggtggc cgcgaccatc gctttccccg gcatcgcggg ctattcggcg
     8161 acgaagtcgg cggtggaccg gctcacccgt gtcgcggcga tggaggccgg gaagctcggc
     8221 tacggcgtgc gggtcaactg cgtgtacccg ggcctggttc cgacgcagat gggcaaccag
     8281 ctcgcggccg acgtggtcga cgtcggcctg ttcccgagca tcgaggacgc gatcggcgcg
     8341 gtggtcgcgc agaccccggc cggacggctc ggcgaggtcg cggacatggc cgacgccgtg
     8401 gtgttcctcg cctccgacgc cgcccgcttc atcaccggcg ccggcctccc ggtcgacggc
     8461 ggcatgggca tgtgaatccc ctccagcgct ccggcacttc aagaaaggaa aaccagtcgt
     8521 gaccaagccc gtcgtcgttt acggcgcgtc cggctacacc ggccgcctga tctgcgaata
     8581 cctccgcgag tacaacgtgg cattcaccgc ggccggccgg gacaaggccc gggtgcaggc
     8641 cgcgctcgac cacattcccg gcctcgacac ggtggaccac gaggtcgtcg aggtctccca
     8701 cgaaaccggt ccgctcaccg agcttttccg ggacgcgtcg gtcgtgtgca acaccgtcgg
     8761 cccgttcagc gaatacggtc acgaggtcgt ccaggcttgt ctcgaatccg gaacgcacta
     8821 cctcgacacc accggtgagc aggattggct gatctccgcc gaagaacgct acggcgcgga
     8881 catggccgcc aagggcctgc tgctctcccc cggcatcgcg cagatgtaca ccaccggcga
     8941 gatcgccgcc aacctctgcc ttgagacgcc cggactggac actttggaca tcctggtgtt
     9001 ctggaagggt taccccacag tcgcttccac gcagaccatc ctggtgaacg ccgcgctgtc
     9061 cgccgcgtat taccttgagc agaacgaata cgcgccatgg cctgccgacg gcggactgca
     9121 agacgtcgcc gtgccggggc accacgagat cgggctcgcg ctgccgtggg gcggtacgtc
     9181 gcatccggtg tggttcaaga acgatccgcg cgtggccaac gtgaaggcgg tcggcggcgt
     9241 gttcgaccgg gcgctgatgc agggcgtgcc gcagatcgtc gcggccgtgc tggagcaggt
     9301 gaaagatctg ccgctggaag aaaaactcaa ggtgctgcac gagcaggcgg ccgcggtccg
     9361 ggacgaaatg ccgccaaggg aaaaccagcg gatcaacacg tcgctggact cggtgcacgc
     9421 gtccggcccg ctcggccgcg cgcattgcgt gatccacggg acctgcaatt acaagcagac
     9481 tgggctgatg caggcgtacg cggcgtactc gctgctgcaa cagccgccgc ggcgggccgg
     9541 gttcgcgtcg gcgtgccagg cgttcgggca ccgcgaactg ctcggcgtgc tgcggtcgtt
     9601 cgggctggtc tcgaatccgg ttctgacggt gcagtcctga gacggggaga cgagcggtgc
     9661 gactgaccga ttacctcgac aagggcgcct cgctgggtcc gtcggctccg tgcctcacca
     9721 cggacggcgg ttcgctcagc tacggcgagg tccagcagct gtcctggcgg gtcgcccgtg
     9781 cgctcgcgcg ttccggcgtg cgaccgggcg acaaggtggc gatcctctcc gccaacgacc
     9841 cggtgtcgtt cgggtgcgtc ttcggcatca gccgggcagg ggcggtctgg tgcccggtca
     9901 acccgcgcaa cgaagcggcg gagaaccggg agttgctcga cctgttcgac tgcacgtgcc
     9961 tgctcgtgca ggagtcgttc gcaccgctcg tctcccgcat cgccggacag ctgccgaagc
    10021 tcgccaccgt ggtgtgcctc gacggctcgt tcgacggcgc gatcccgctg gccgaatggc
    10081 tcgacgggct tgccgattcg ccctggcaag cggatccgcc ggacgacacg gcgatgctcg
    10141 tcggcaccgg cggcaccacc ggacggccga agggcgtggt gctgaccggg cacaacgtcg
    10201 aaaccatgtc ggcgctgacg ttgatgagct acccgttcca gccgcgtccg cgctacctcg
    10261 cgctggcccc gctcacgcac gccgccgggg tgctgtgctt cccggtcctg gcgctgggcg
    10321 gcgaaatcgt ggtgatgccg tcgccggagc tcggccggtt cctcgaactg atcgaacggc
    10381 accggatcac gcacactttc ttgccgccga cgctgattta cctgctgctg gaccatcctg
    10441 gactgtccac aacagacttc agctcgctgg aatgcctgtg gtacggggcc gcgccgatgt
    10501 ccgctgcccg gctggaggag gcgatcgccc ggatcggtcc ggtgctcggg cagctgttcg
    10561 ggcagagcga ggcaccgatg atgatttcca cgctggcccc ggccgaccat ttccatccgg
    10621 acggttccct ggcgcgcgaa cgcttcacct ccgcgggccg tccgacgccg ctgacgcagg
    10681 tggcgatcat ggccgaagac ggaaccctgc tgccgcgcgg cgaacgcggc gagatcgtcg
    10741 tgcgcgggcc gctggtgatg ccgggctact accgcaatcc cgaggccacc gcggacgt
//
