LOCUS       NZ_CP091381            10348 bp    DNA     circular CON 18-AUG-2024
DEFINITION  Amycolatopsis sp. FU40 chromosome, complete genome.
ACCESSION   NZ_CP091381
VERSION     NZ_CP091381.1
KEYWORDS    RefSeq.
SOURCE      Amycolatopsis sp. FU40
  ORGANISM  Amycolatopsis sp. FU40
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Amycolatopsis.
REFERENCE   1  (bases 1 to 9944196)
  AUTHORS   Reisman,B.J. and Bachmann,B.O.
  TITLE     Biosynthesis of Apoptolidin Family Glycomacrolides
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9944196)
  AUTHORS   Reisman,B.J. and Bachmann,B.O.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2022) Chemistry, Vanderbilt University, 1234
            Stevenson Center Drive; Room 7330, Nashville, TN 37235, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 23-JAN-2022
            Assembly Method                   :: Flye v. 2.9
            Assembly Name                     :: NFU40_flye_2.9
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 47.0x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_021903635.1-RS_2024_08_18
            Annotation Date                   :: 08/18/2024 04:30:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,491
            CDSs (total)                      :: 9,417
            Genes (coding)                    :: 9,360
            CDSs (with protein)               :: 9,360
            Genes (RNA)                       :: 74
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 7 of 57
            Pseudo Genes (incomplete)         :: 50 of 57
            Pseudo Genes (internal stop)      :: 5 of 57
            Pseudo Genes (multiple problems)  :: 5 of 57
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:13:09
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 5302345
            Orig. end                         :: 5312693
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP091381.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Brian O. Bachmann,
            Vanderbilt University.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10348
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="26"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10348
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10348
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5348](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5348)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    811..831
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 22.95"
                     /tool="antismash"
     gene            950..1975
                     /locus_tag="L3Q65_RS25210"
                     /old_locus_tag="L3Q65_25210"
     CDS             950..1975
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1014: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /locus_tag="L3Q65_RS25210"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L3Q65_25210"
                     /product="LysR family transcriptional regulator"
                     /protein_id="WP_182891441.1"
                     /transl_table=11
                     /translation="MIGAERRNRLGRVVLDAIWSDGGRLTLLQLRALYAVANEGSFAAA
                     AVELGCSQPAVSQRIASIERRLGVVLVRRTSGQRQVRLTDAGIALVNAAEQVQVILRTA
                     TRHIQDMRDGREPTVRIGVFSSVAVRVMPLVLAAMRTQHLPCRIKPIEVDDDVRLQELL
                     RFGEIDIAFVIFPLQSGEAHGLELFSDPFILLAPRNAPLASVDRVRPADLRGLPMVCLT
                     SEQLEVAAELRRQGIEPNWVFDTDKIHVVEPLVAADVGYSIVPQLALGQRHPGVTRFSL
                     SEIVSPREIGIAWPPHHPRLGLLLRVADVIRGVCADPAHILPPIDAPGDASVTAGDALL
                     R"
     gene            complement(1943..2626)
                     /locus_tag="L3Q65_RS25215"
                     /old_locus_tag="L3Q65_25215"
     CDS             complement(1943..2626)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:TIGR03608.1"
                     /locus_tag="L3Q65_RS25215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L3Q65_25215"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_182891440.1"
                     /transl_table=11
                     /translation="MEFLCELRGIRKQYDLHPVLDDFALGVEPGEFTALTGASGAGKST
                     VLNLLGLLESPDAGEVRLFGERAPRPRSRAANLLRRNRLGYLFQNFALIDSETVEHNLE
                     VALTYAKRGTPKRDRVKEALAQVGLRRSEHRKIHSLSGGEQQRVAVARLLLKPCDLVLA
                     DEPTGSLDARNRDRVLDLLEKLNEAGKTIIVATHDEVVAERCSRVVDLSGPAAESTQER
                     VASGH"
     misc_feature    complement(2610..2625)
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 20.74"
                     /tool="antismash"
     gene            complement(2630..3937)
                     /locus_tag="L3Q65_RS25220"
                     /old_locus_tag="L3Q65_25220"
     CDS             complement(2630..3937)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="L3Q65_RS25220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="L3Q65_25220"
                     /product="hypothetical protein"
                     /protein_id="WP_237596823.1"
                     /transl_table=11
                     /translation="MNHRTRHRSPFGLAIAAPAVVKAVCGAILAVLAVVVWQQFSELAK
                     QQQLLSPWEKVQGYGVFYPRLIGNDQQEMETGGNASSVAEARDLYPVLDRAGALFVDAA
                     NYEPGTPPDPTSRWPVPPIRVNTHYLEQYPILDTARKPITVAADDPAWVVAVPEQFKPR
                     EAQIRQLLQETRTGGPGITGATQAEQRITGDQPQFTSQRVRIVWTASGQGVFSYDPQVN
                     PGHGNLITDPIVEIMTPANSLAVDRLNAITGGLDTGLKVRVGDDPAGTLAALGPKLKEL
                     KLDDNLQHLVTVHEAMAAQLDQVRGGITRIAVFAGAALLVLVALTAVIVLIGFDRLRRR
                     LTVRRLHGIGFARSYRELLIALGGTWLGQTVLAGIGLAVLAMNTLSPADAAAGPLDRLP
                     ELTAVSVLSLAIEAMFVVVTARILERRNTVKRLKEL"
     gene            complement(3934..4887)
                     /locus_tag="L3Q65_RS25225"
                     /old_locus_tag="L3Q65_25225"
     CDS             complement(3934..4887)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="L3Q65_RS25225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="L3Q65_25225"
                     /product="hypothetical protein"
                     /protein_id="WP_237596824.1"
                     /transl_table=11
                     /translation="MRLILSLLFAGILFAIPAVVLMDVVLEQEAHSETAADRVGVPFVW
                     PERPQVADPETTLRILAGAAEATGATVVRTTVSTQADRKRITHYVLLGGDRTALFSEFA
                     LAQGRWLSPVESRSGAATVSTSGSRGNVGVPAVFADRYELAFAPLRQAFDFLPSSGRYV
                     VDAGPATDRFLALVRERLAEAGVTVSDLTPPNRGEQPSETGPGLRILAYLLAGASTVVV
                     AFRLLREGKRVGVLRLIGHPTTRIWYDVVGRMQIGTTLAELGACAAVVLVVPGVDSSFL
                     YAVAPVTAAGFAATLGIGLLVVHRVRISDLVKGSLQ"
     gene            complement(5001..5348)
                     /locus_tag="L3Q65_RS25230"
                     /old_locus_tag="L3Q65_25230"
     CDS             complement(5001..5348)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Lactococcin_972"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF021219.4"
                     /locus_tag="L3Q65_RS25230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L3Q65_25230"
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_182891437.1"
                     /sec_met_domain="Lactococcin_972 (E-value: 1.2e-08,
                     bitscore: 34.4, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGSASLKIARVASVASLAVVMSAGIVSAANASPSREAVPAGCGSA
                     GGGTWCHGSGRDGVWKGCYSNYNHPRNYHSSTAAIGAANDKRYASAGSWSNAHARAGWA
                     YTCYTYYNPNA"
     gene            5946..6542
                     /locus_tag="L3Q65_RS25235"
                     /old_locus_tag="L3Q65_25235"
     CDS             5946..6542
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /locus_tag="L3Q65_RS25235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L3Q65_25235"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_182891436.1"
                     /transl_table=11
                     /translation="MSRAAEPETPVERPGAYRSRFTGALESLLAEKRFPKITIGDLVGR
                     AHTSRRGFYEQFDSKEACLVALLKEAAATGSAQAAAAVDRTAPWQTQLRQLVTAWIGVV
                     DARPAPMLSYIRDVPLLDAPISADLNQDAYVNVARAISAGAEFRESGGVALTRTRALIL
                     FGGLEKLAEDALQHGAAVHEHTEEAVRAAILLAAA"
     gene            complement(6547..7116)
                     /locus_tag="L3Q65_RS25240"
                     /old_locus_tag="L3Q65_25240"
     CDS             complement(6547..7116)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF044451.1"
                     /locus_tag="L3Q65_RS25240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L3Q65_25240"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_237596825.1"
                     /transl_table=11
                     /translation="MRRTNEERSRATRHDLVEAARGLFAEHGYAGTSLAQIAAAAGTTT
                     GAVYHHWENKQELFHAVVEQVHADLHRVLPARSGGTGTPADRLKASGSLFLRRCADPAV
                     GRILLVDGPSVLGQEKWRNLDEQWWRGPAVALLREAGGPEELAVALLGSLTALGQEIAH
                     DPRPVRIRRCQQVYRTLVDAVSVQVS"
     misc_feature    complement(7131..7147)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 16.71"
                     /tool="antismash"
     gene            7148..7585
                     /locus_tag="L3Q65_RS25245"
                     /old_locus_tag="L3Q65_25245"
     CDS             7148..7585
                     /EC_number="3.1.2.-"
                     /GO_function="GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /locus_tag="L3Q65_RS25245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L3Q65_25245"
                     /product="PaaI family thioesterase"
                     /protein_id="WP_220507174.1"
                     /transl_table=11
                     /translation="MDTVRAQEIIDSSPFGPWWGLVVKSVEPGVATLSLPARPEFFRPG
                     DVLQGGCAMTLADVTCWIAILSALGPEEPAVTVNMTTAFLGAARSDLTCESSLTRTGRR
                     TIYGTATVTDDAGRQVSHHTLTYLRPGRQPSTQPGSPERAE"
     gene            complement(7482..8825)
                     /locus_tag="L3Q65_RS25250"
                     /old_locus_tag="L3Q65_25250"
     CDS             complement(7482..8825)
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1184: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005453988.1"
                     /locus_tag="L3Q65_RS25250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L3Q65_25250"
                     /product="MFS transporter"
                     /protein_id="WP_237596826.1"
                     /transl_table=11
                     /translation="MELDHPTTAPPCPVLRVMAVFRQRFPLRPFYARPVHSDHIRGPVV
                     WFISLAAALGTAAIYPLQPAIAEVGGALGTSLGSVGVALACGPVGYLCGLALLVPLVDQ
                     FPPRYVLSAQFGALAVALGLNAMVGQVWLLGLVLGVLGACSSAGAGLSSVTARLASPDR
                     RATALGIVTAGISAGILAGRIVGGWLSDLLGWRAMLGVLAAGCAVVAVAVLLVLPSVSG
                     GNNRGYLATLRTIPGLYLRYPVLRLAAVRGTLWFFAFCAVWSGIAVALSRPPFSYTAEQ
                     IGLYAFAGILGMLATRIAGAWTDRVGARRVVLCGLGLAGVSSAVLAVALPSTVSTLICL
                     ALFDAGLFAAQVANQSTVLAIEPGAPARFNSAYMVVYFAGGSLGTAFGATAAERIGWPL
                     TALVTAAVLVAAGVVTLLARESPAESTAVAPDEGRSACDGTPAAPRRR"
     misc_feature    complement(8352..8354)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            8811..9452
                     /locus_tag="L3Q65_RS25255"
                     /old_locus_tag="L3Q65_25255"
     CDS             8811..9452
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020629667.1"
                     /locus_tag="L3Q65_RS25255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L3Q65_25255"
                     /product="hypothetical protein"
                     /protein_id="WP_370648117.1"
                     /transl_table=11
                     /translation="MIEFHDDPTAGAALQTVAEPTGRVMTGERARQIDVSHLPRDVVDL
                     IDALGPGENLILTRDGDPVATISSAGRAPEPPRTDYDDVTVVATAMKLSASARMSLSAE
                     LGSDYLVLDLHSAPATADVLLVPPVSPQLIGGLRAQFPKARVVLAEVEDAELGVSYHGP
                     VRRLLDAGAETYLTSANVPHLARQLDHAVTQRQQLAGGAAAPLEISPADS"
     gene            complement(9456..9668)
                     /locus_tag="L3Q65_RS47375"
     CDS             complement(9456..9668)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /locus_tag="L3Q65_RS47375"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="SDR family oxidoreductase"
                     /protein_id="WP_255750829.1"
                     /transl_table=11
                     /translation="MNSVGVGYPRTDLTAALLEVPHNLEHNLSRTALGRIGEVEDIADA
                     VAFLASDDARWIAGTQLDVSGGVGR"
     gene            complement(9734..10216)
                     /locus_tag="L3Q65_RS47380"
     CDS             complement(9734..10216)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /locus_tag="L3Q65_RS47380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_370648118.1"
                     /transl_table=11
                     /translation="MRQGSRAGTLRWGSSATRSPSPPGAAGASAGRLARDGALVAVHYG
                     RNETAARGDGRCDQRGRRPGLPLGAELGVPDDAAALTAALDTDLDILVHNASRYLLHRP
                     IEVLTPEEFDEMFAVNVKAPFFLPQRLLPRLRDGGRIIAVSSVATGSRRRTASRIR"
ORIGIN
        1 ggcagcgggc acgcggtggt gaacccgggc agcactccgc ggatcaccct gatcgggcac
       61 atcgacgaaa tcgggctgag cgtcagccac atcgacgacg ccggacggct gtgggcggtc
      121 agcatcggct gctgggacgc cgcgatcctg tacggccagc atgtcgtggt gctcgccgag
      181 aacggccggg tgccgggcgt gatcggcacc gcgccgccgc atctgaacga cggggattcc
      241 tcgcccaaga tggccgatct gtggatcgac atcggcgcca ccagccgggc cgacgcgcaa
      301 tcggtgatca ccgtcggcga cccggtggtg ttcggcgcgt ccccggtcga cctgcgcaac
      361 ggccggatcg ccgctccggc gctggacaac cggctcggcg cgctggcggt cctcgaagcg
      421 gcacgcgcgg cagcagcggc gtcggtcacc gccgagatct ccgtggtcgc cagcgtgcgc
      481 gaggaaacga gctaccacgg cgcgatggga gccgtgtacg cgacggaacc gaacttcgcg
      541 ctcgcgatcg acaccacctt cgccagcgac atcccggagg acggcatcga acacaaaacc
      601 ggcgaccacc ggctcggctc cggcccggcg atcggccgcg gcgccgtcac cgacgaaccc
      661 atggcccgca gcctgcgcac gctggccaag gagctcggaa tcccttacac catcgaggcg
      721 tacggcgacg aaagcggctc cgacgccgac gccatggccg ccgtcgccgc cgggatccgg
      781 tgcggcgtca tcaacatccc gatccggcac atgcacagct ccggcgaagt ggcggatctc
      841 gccgatctgg cgagcacgat cgagctcgcc accgcgttct gcctcgccgg agggacccga
      901 tgagccgcac gcgacgggaa gcctgagttc ctggcaagga ggccagccca tgatcggcgc
      961 ggaacgacgg aaccggctcg gccgggttgt gctggacgcc atctggtccg acggcggccg
     1021 cctcacgctg ctgcagctga gagcgctgta cgcggtcgcg aacgaaggct cgttcgcggc
     1081 cgccgcggtg gagctcggct gcagccagcc cgcggtcagc cagcgcatcg cgtcgatcga
     1141 acggcggctc ggcgtcgtgc tggtccgccg gacgtccggg cagcgccagg tgcggctcac
     1201 cgacgccggg atcgcgctgg tcaacgccgc cgaacaggtg caggtcatcc tccgcaccgc
     1261 gacgaggcac atccaggaca tgcgcgacgg ccgcgaaccg accgtccgga tcggcgtgtt
     1321 ctcgagcgtc gcggtgcggg tgatgccgct ggtgctggcc gcgatgcgca cccagcacct
     1381 cccgtgccgg atcaaaccca tcgaggtcga cgacgacgtc cgccttcagg aactgctgcg
     1441 cttcggcgag atcgacatcg cgttcgtcat cttccccctg caaagcgggg aagcacacgg
     1501 gctggaactg ttcagcgacc cgttcatcct gctcgcgccc cggaacgccc cgctggcctc
     1561 cgtcgaccgc gtccgcccgg ctgacctgcg cggtctgccc atggtgtgcc tgaccagcga
     1621 acaactcgag gtcgccgccg agcttcgcag gcagggcatc gagccgaact gggtcttcga
     1681 cacggacaag atccacgtcg tggaaccgct ggtcgccgcg gatgtcgggt attcgatcgt
     1741 gccgcaactg gcattggggc aacggcatcc gggcgtcacc cggttctcgc tgagcgagat
     1801 cgtgtcgccg cgagagatcg ggatcgcctg gccgccgcat catccgcggc tcggtttgct
     1861 gctccgggtc gccgatgtca tccggggcgt gtgcgcggac ccggcgcaca tcctgccgcc
     1921 gatcgacgcg cccggagacg cctcagtgac cgctggcgac gcgctcctgc gttgattccg
     1981 ctgcggggcc ggaaagatcc accacccgcg aacaccgctc agccaccacc tcgtcgtgcg
     2041 tcgccacgat gatcgtcttg cccgcctcgt tgagcttctc caggagatcc aagacccggt
     2101 cccggttcct ggcatcgagc gaacccgtag gttcgtccgc caggacgaga tcgcacggtt
     2161 tgagcagcag ccgcgccacc gcgacgcgtt gctgttcgcc gccggacagc gagtggatct
     2221 tccggtgttc cgaccggcgc agcccgacct gcgcgagcgc ctccttgacg cggtcccgct
     2281 tcggcgtccc ccgcttggcg taggtgagcg cgacctccag attgtgttcg accgtctcgc
     2341 tgtcgatgag cgcgaaattc tggaacagat accccagccg gttgcggcgc agcagattcg
     2401 ccgcccgcga gcgcggccgc ggcgcgcgct cgccgaaaag gcggacctcg cctgcgtcag
     2461 gcgattccag caggccgagc aggttcagca cggtcgactt cccggccccg ctcgcgccgg
     2521 tcagcgcggt gaactcgcct ggctcgacgc cgagcgcgaa atcgtccagg accgggtgga
     2581 gatcgtactg tttccggatt ccgcggagtt cgcacaagaa ttccatgggt cacagctcct
     2641 tcaagcgttt gaccgtgttc cggcgctcca ggatccgagc ggtcacgacc acgaacatcg
     2701 cctcgatcgc cagcgagagc actgataccg cggtgagttc cggaagccgg tccagcgggc
     2761 ccgccgcggc gtcggccggc gacagggtgt tcatcgccag cactgccagc ccgatcccgg
     2821 ccagcacggt ttggcccagc cacgttccgc ccagggcgat cagcagttcc cggtacgaac
     2881 gggcgaagcc gatcccgtgg agtctgcgca cggtgagccg tcggcgcagg cggtcgaagc
     2941 cgatgagcac gatcaccgcg gtcaaggcga ccaggacgag cagcgccgcg ccagcgaaca
     3001 cggcgatccg cgtgatcccg ccgcgcacct ggtcgagctg cgcggccatg gcctcgtgca
     3061 cggtgacgag atgctggaga ttgtcgtcga gcttgagttc cttcagtttc ggaccgagcg
     3121 cggccagtgt gcccgccgga tcgtcgccga cgcggacctt cagcccggtg tccaagccgc
     3181 cggtgatcgc gttgagccgg tcgacggcga ggctgttggc cggggtcatg atctcgacga
     3241 tcgggtcggt gatcaggttg ccgtgcccgg gattgacctg cgggtcgtag ctgaacacgc
     3301 cttgcccgga ggcggtccag acgatccgga cgcgctggct cgtgaactgc ggctggtcgc
     3361 cggtgatgcg ttgttccgct tgcgtcgcac cggtgattcc cggaccgccg gtgcgtgttt
     3421 cctgaagaag ctggcggatc tgcgcttccc tcggcttgaa ttgctcggga acggcgacga
     3481 cccacgccgg gtcgtcggcg gcgaccgtga tcggcttccg cgcagtgtcc aggatcgggt
     3541 actgctcaag gtaatgcgtg ttgacccgga tcggcggcac cggccaccgc gacgtcgggt
     3601 ccggcggcgt gcccggctcg tagttggcgg cgtcgacgaa gagcgcgcct gcccggtcga
     3661 gcaccggata gaggtcccgg gcttccgcga ccgacgaggc gttgccgccg gtctccattt
     3721 cctgctggtc gtttccgatc aagcgcgggt agaagacccc gtagccctgg actttctccc
     3781 atggcgaaag caattgctgt tgcttcgcca gttcggagaa ctgctgccag accaccacgg
     3841 ccagcaccgc gaggatcgcg ccgcataccg ccttgaccac ggccggtgcg gcgatcgcca
     3901 aaccgaacgg cgaacggtgt cgcgtcctgt gattcactgc agacttcctt tgacgagatc
     3961 cgaaatgcgc acccggtgga cgacgagcag cccgatgccc agcgtcgccg cgaatccggc
     4021 tgccgtcacc ggcgccaccg cgtacaggaa cgacgagtcc acgccgggca cgacgaggac
     4081 gaccgcggcg cacgcgccca gctccgcgag agtggtaccg atctgcattc tcccgacgac
     4141 gtcgtaccag atccgcgtcg tcggatgccc gatcaggcgg agcacgccca cccgttttcc
     4201 ctcgcgcagc aaccggaacg caacgaccac agtggacgcg cccgccagca gatacgccag
     4261 gattcgcagc cctggaccgg tttccgacgg ctgctcaccg cggttgggcg gagtcagatc
     4321 ggagaccgtg acacccgctt cggccaaccg ctcgcggaca agtgcgagga agcggtccgt
     4381 cgccggtccc gcgtcgacca cataccggcc ggaactgggc aagaaatcga acgcctgacg
     4441 cagcggcgcg aacgcgagct cgtaccggtc cgcgaaaacc gctgggacac caacgtttcc
     4501 acggctgccc gacgtcgaaa ccgtcgctgc cccgctccgc gactccacgg gactcagcca
     4561 acgtccttgc gcgagagcga attcgctgaa caacgccgtc cgatcgccgc cgagcaacac
     4621 gtaatgcgta atgcgcttgc ggtccgcttg agtgctcacg gtggtgcgca caaccgtggc
     4681 tccagtcgcc tccgccgctc cggcgagaat gcgcagcgtc gtctccggat ccgcgacctg
     4741 tgggcgttcc ggccagacga acggcactcc gacccggtcc gccgcggtct cgctgtgcgc
     4801 ttcctgctcg agaaccacgt ccatcagaac gaccgccgga atggcgaaca ggattcccgc
     4861 gaaaaggaga gagaggatca gtcgcacgga aagctcgctc tcgtcgctgc tgcgtgcctg
     4921 ctgcggtgga ggtgcggtgc cgggaccccg tcggcccggc accgcaccgt ggtgatcatg
     4981 cccggagcgt caggccacga tcacgcgttg gggttgtagt acgtgtagca ggtgtaggcc
     5041 cagcccgccc tggcgtgcgc gttggaccag gaacccgcgg acgcgtaccg cttgtcgttg
     5101 gcggcgccga tggccgccgt cgagctgtgg tagttccgcg ggtggttgta gttcgagtag
     5161 cagcccttcc agacgccgtc acggccggaa ccgtggcacc aggtgccgcc gccggcgctg
     5221 ccgcagcccg ccgggacagc ttcccgggac ggcgacgcat tcgcggccga aacgattccc
     5281 gcggacatga cgaccgcgag cgaagcgacc gaagccaccc tggcgatctt cagagacgca
     5341 gaccccattg tcatgatttc cccattcttt ttcctgattt cccctgagcg gaccgctccc
     5401 ctgccctgag cgaaaaacat tcaggttctg ccctcggcgc ggtaatgcga ggacgggaac
     5461 agccgcgtcg tccccgccgg cccccgacgc cggcgggacg tatgcgccgg accattccgg
     5521 caagcaggca cggtcggccg ggacaggtgc gcacgcaccc gcaggcaacc cgctgacctg
     5581 cggaaacagc tatttcgtca cagagttgtg ctcaccgagt gccgcgtctc gtgcgcggcg
     5641 cgaagaaaaa cggtgaaaac acctggatcc ccctagcgct cgactcccca atctgacgct
     5701 ggaacaaaac actaccgaac gtacccgcaa tcggccaaac gtctttccgc tatgtcactg
     5761 agatggcgta gccgctcccg gagcgtcgcc ggcggtactg ccacccggac cagccagcgc
     5821 cgccgaatgc cgccgcgctc tttccttccc ggaattccgc ggatatcccg ctcccgtttc
     5881 ggaataccgg ccgccgccgg actggtagaa tcgccggaca accgcggaca ggaaggaccc
     5941 gcgcggtgtc acgcgctgca gagccggaaa cgcccgtcga gcgccccggc gcctaccgga
     6001 gccgcttcac cggcgcgctg gagtcgctgc tcgcggaaaa acgcttcccg aagatcacga
     6061 tcggcgacct cgtcggccgc gcgcacacct cccgccgcgg cttctacgaa cagttcgatt
     6121 ccaaggaagc ctgtttggtc gccctgctca aggaagccgc cgcgaccggc tcggcacagg
     6181 cagccgcggc ggtcgaccgc accgcgccct ggcagacgca actccgccag ctcgtcaccg
     6241 cgtggatcgg cgtcgtcgac gcccggcccg cccccatgct gagctacatc cgcgacgtgc
     6301 cgctgctgga cgccccgatc tccgcggacc tgaaccagga cgcatacgtc aacgtcgccc
     6361 gcgccatctc ggctggcgcc gaattccgcg agtccggcgg cgttgcgctg acgcggaccc
     6421 gcgccctgat cctgttcggc ggcttggaaa aactggccga ggacgcactg cagcacggtg
     6481 ccgcggtgca cgagcacacc gaggaagcag tgcgggccgc gattcttctt gccgctgcgt
     6541 gatatctcag ctcacctgaa ctgacaccgc gtccactagc gtccgatata cctgctgaca
     6601 ccgccgaatc cgcaccggac gcggatcgtg ggcgatctcc tgtcccagcg cggtaagcga
     6661 tcccagcaac gcgaccgcga gctcttcggg accgcccgcc tcgcgcagca acgcgacagc
     6721 cggtccccgc caccactgtt cgtccaagtt tcgccacttt tcctggccca gcacggacgg
     6781 accgtcgacc agcaggatcc gccccaccgc cgggtccgcg caccggcgga gaaagagcga
     6841 gccggacgcc ttgagccgat cggccggcgt tcccgtgcca ccggagcggg cgggcaacac
     6901 ccggtgcaga tcggcgtgca cctgctcgac caccgcgtgg aacaactcct gcttgttctc
     6961 ccagtggtgg tacaccgcgc cagtggtggt cccggccgcc gcggcgatct gggccagcga
     7021 ggtgcccgcg tagccgtgct cggcgaagag cccgcgcgcg gcctcgacca ggtcgtggcg
     7081 agtggcccgg gaccgttcct cgttcgttcg ccgcatccac ctacctaatc atgttatgtt
     7141 tctgcgcgtg gacacggtgc gggcgcagga aatcatcgac agcagcccgt tcgggccgtg
     7201 gtggggtttg gtggtgaagt cggtcgaacc gggcgtcgcg accctttccc tgcccgcccg
     7261 tccggagttc ttccgtcccg gcgacgttct gcagggcggc tgcgccatga cgctcgccga
     7321 cgtcacctgc tggatcgcga tcctctccgc tctcgggccc gaggaaccgg ccgtcacggt
     7381 gaacatgacg accgcgttcc tcggcgcggc acggtcggat ctgacctgcg agtcgtccct
     7441 gacccgaacc ggccgccgga cgatctacgg caccgcgacg gtcaccgacg acgcggggcg
     7501 gcaggtgtcc catcacacgc tgacctacct tcgtccgggg cgacagccgt cgactcagcc
     7561 gggctctccc gagcgagcag agtgacaacc cccgcggcca cgagcaccgc agcggtaacc
     7621 aacgccgtca gcggccaccc gatccgctca gccgcggtag caccgaacgc ggtgccaagg
     7681 cttcccccag cgaaatacac caccatgtaa gcactattga accgcgcagg cgcaccaggc
     7741 tcgatcgcca gcaccgtgct ctgattcgcc acctgcgcgg cgaacagccc cgcgtcaaac
     7801 agcgccaagc agatcaacgt cgaaacagtg ctgggcaagg caacggcaag caccgcgctg
     7861 gaaactccag caaggcccag cccgcaaagg acaacccgac gggcccccac gcgatcagtc
     7921 cacgcacccg cgattcgagt ggccaacatc ccgagaatgc cagcaaaagc gtacaacccg
     7981 atctgctcgg cagtatacga aaagggcggc ctggacagag caaccgcgat cccagaccac
     8041 accgcacaga acgcgaagaa ccaaagcgtc ccccgaaccg ccgcgagccg aagcaccgga
     8101 tagcgcaggt atagccccgg aatcgtgcgc aacgtggcga ggtagccacg attgttccca
     8161 ccggacaccg aaggaagcac gagcagcacc gccaccgcga ccaccgcgca tccggcagcg
     8221 agaacgccca gcatcgcgcg ccagccaagc aggtcggaca gccaaccgcc gacgatgcgg
     8281 cccgcgagga tgcccgctga gatcccagcg gtgacgattc ccagcgcggt ggctcgccgg
     8341 tccgggctcg ctaaccgagc ggtcactgaa ctcagcccgg cgcctgccga cgagcacgca
     8401 ccgagcacgc cgagcaccaa gccgagcaac cagacctggc cgaccatcgc gttcagcccc
     8461 aacgcgaccg cgagcgcgcc gaactgcgcc gacaggacgt agcgcggcgg aaactggtcg
     8521 acgagcggca ccagcaacgc gagtccgcac aggtagccga ccggtccgca ggcgagtgcg
     8581 acgccgaccg agccgagcga cgtgccgagt gctccgccga cctcggcgat cgcgggctgc
     8641 aacgggtaga tggccgcggt gcccagcgcg gcggcgagtg aaatgaacca gacgacgggg
     8701 ccgcgaatgt gatccgaatg cacgggtcga gcgtagaaag ggcggagcgg gaaacgctgg
     8761 cgaaaaaccg ccatcacccg aagcaccgga caaggcggcg cggttgtggg atgatcgagt
     8821 tccatgacga tcccaccgcg ggcgccgcgc tccagacagt tgccgagccg accggccggg
     8881 tcatgaccgg cgagcgggcg cggcagatcg acgtcagcca ccttcccagg gacgtcgtgg
     8941 acctgatcga cgcgctcggg ccgggcgaaa acctgatcct caccagggac ggcgacccgg
     9001 tcgcgacgat ctccagcgcc ggccgcgcgc ccgaaccgcc gcggacggac tacgacgacg
     9061 tgaccgtggt cgccaccgcg atgaagctgt cggcctcggc ccggatgtcg ctgtccgccg
     9121 aactcggctc cgactacctc gtgctggacc tgcactcggc gccggccacc gccgacgtgc
     9181 tgctcgtgcc gccggtcagc ccgcagctga tcggcggcct ccgggcgcag ttcccgaagg
     9241 cgcgggtcgt gctcgccgag gtcgaggacg cggagctggg cgtcagctac cacggaccgg
     9301 tccggcggct gctcgacgcc ggcgcggaga cctacctgac ctcggcgaac gtcccgcatc
     9361 tggcgcggca gctcgaccac gcggtcaccc agcggcagca gctcgccggc ggggcggccg
     9421 cgccgctgga gatcagcccc gcggactcgt gaccgctacc ggccgacgcc gccggacacg
     9481 tcgagttgcg tgccggcgat ccagcgagcg tcgtccgagg cgagaaaggc gaccgcgtcc
     9541 gcgatgtcct cgacctcgcc gatgcggccg agcgcggtcc ggctgaggtt gtgttccagg
     9601 ttgtgcggga cttccagcag cgctgctgtc aggtcggtcc gcgggtagcc gaccccgacg
     9661 gaattcaccg tgatcccgcg gggagccagg tgctaggcca gggtttggct gaacatgtcg
     9721 agcgcgccct tggtcatcgg atacgcgatg ccgtccggcg ccgtgacccg gtggcgaccg
     9781 acgagacggc gatgatccgc ccgccgtcgc gcagccgggg caagagtcgc tgaggcaaga
     9841 agaacggggc tttgacgttg accgcgaaca tttcgtcgaa ttcctccggg gtgaggacct
     9901 cgatcggccg gtgcaggagg tagcggctgg cgttgtgcac caggatgtcg aggtctgtgt
     9961 ccagtgcggc ggtgagggcc gccgcgtcat cgggaacgcc gagctcggca cccaacggaa
    10021 ggcccggtcg cctgcctcgt tgatcgcagc gaccgtctcc ccgggcagcg gtctcgttgc
    10081 ggccgtagtg caccgcgacc aaggcgccat cgcgagccag gcgtccggcc gacgccccgg
    10141 ctgcccccgg tggcgagggc gaccttgtcg ctgagcttcc ccatctcagc gttcccgctc
    10201 ggctgccttg acgcacagct cggtgccgcc gtgcaaaggc agcgcgaccg acacgaatcc
    10261 gttagccggg tcgcgaatcc aggcgaggaa atcgggttcg gcgttgtcat tcagcacata
    10321 gccgccgatc cggaggtgcg gtgcgacg
//
