LOCUS       NZ_CP091381            10813 bp    DNA     circular CON 18-AUG-2024
DEFINITION  Amycolatopsis sp. FU40 chromosome, complete genome.
ACCESSION   NZ_CP091381
VERSION     NZ_CP091381.1
KEYWORDS    RefSeq.
SOURCE      Amycolatopsis sp. FU40
  ORGANISM  Amycolatopsis sp. FU40
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Amycolatopsis.
REFERENCE   1  (bases 1 to 9944196)
  AUTHORS   Reisman,B.J. and Bachmann,B.O.
  TITLE     Biosynthesis of Apoptolidin Family Glycomacrolides
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9944196)
  AUTHORS   Reisman,B.J. and Bachmann,B.O.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2022) Chemistry, Vanderbilt University, 1234
            Stevenson Center Drive; Room 7330, Nashville, TN 37235, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 23-JAN-2022
            Assembly Method                   :: Flye v. 2.9
            Assembly Name                     :: NFU40_flye_2.9
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 47.0x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_021903635.1-RS_2024_08_18
            Annotation Date                   :: 08/18/2024 04:30:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,491
            CDSs (total)                      :: 9,417
            Genes (coding)                    :: 9,360
            CDSs (with protein)               :: 9,360
            Genes (RNA)                       :: 74
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 7 of 57
            Pseudo Genes (incomplete)         :: 50 of 57
            Pseudo Genes (internal stop)      :: 5 of 57
            Pseudo Genes (multiple problems)  :: 5 of 57
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:13:09
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 3935577
            Orig. end                         :: 3946390
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP091381.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Brian O. Bachmann,
            Vanderbilt University.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10813
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="21"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10813
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10813
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5813](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5813)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(2..802)
                     /locus_tag="L3Q65_RS18890"
                     /old_locus_tag="L3Q65_18890"
     CDS             complement(2..802)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_function="GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005452804.1"
                     /locus_tag="L3Q65_RS18890"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L3Q65_18890"
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="WP_237600435.1"
                     /transl_table=11
                     /translation="MTAATMTRTRRDDYRHCAVLFEEISHLAEDTDEHSRLRRRLIEEH
                     LPLAEHIARRFARRGQPSEDLLQVARIGLINAVDRYDPARQTDFVAFAVPTVMGEVRRY
                     FRDHTWSVRVPRRLKELHVTIGQAATDLGQRIGRAPTARELADHLDLDLDEVTEALHAG
                     NAYQAASIDYPAHDETGSLALADTLGDEDPEIEKLEDRETLKPLLRSLPERERAIVVMR
                     FFSNMTQSQIADRVGLSQMQVSRLLARTLSTLREQLTEAATATR"
     misc_feature    complement(871..886)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 16.36"
                     /tool="antismash"
     gene            complement(1027..1455)
                     /locus_tag="L3Q65_RS18895"
                     /old_locus_tag="L3Q65_18895"
     CDS             complement(1027..1455)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF039717.3"
                     /locus_tag="L3Q65_RS18895"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L3Q65_18895"
                     /product="DUF6292 family protein"
                     /protein_id="WP_237600436.1"
                     /transl_table=11
                     /translation="MIATPHVPPGNPHAVLAPLRGYLAEVARALGVGPEFCALDPGPPV
                     SAYVALDGHLPGCPGRDVALLWDEVHGWSTAVETHSGEDLIVLRYFGGATVVPPPAQLL
                     RFVTALRAAITASGSSRRPSCVPSAVPPNSTLCCVCAM"
     gene            complement(1478..1972)
                     /locus_tag="L3Q65_RS18900"
                     /old_locus_tag="L3Q65_18900"
     CDS             complement(1478..1972)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009080014.1"
                     /locus_tag="L3Q65_RS18900"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L3Q65_18900"
                     /product="hypothetical protein"
                     /protein_id="WP_182893134.1"
                     /transl_table=11
                     /translation="MGSQLAATWVSAIGSAGSLLGFASYVWIMLLNRKDQAEVRQEQQA
                     QSVSAWLDEGARHDRDEDYVVCAHNSGDAPVFDCSLEVSRPDEEEPHCARFAIIPPGAT
                     AVRALPFGEDAMPVDDLYSAAAVPGLSFTDSRGGHWTRDTSGVLSRADRRSVRGRWRPG
                     S"
     misc_feature    2019..2034
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: weak, score: 20.56"
                     /tool="antismash"
     gene            complement(2144..3574)
                     /locus_tag="L3Q65_RS18905"
                     /old_locus_tag="L3Q65_18905"
     CDS             complement(2144..3574)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009080016.1"
                     /locus_tag="L3Q65_RS18905"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L3Q65_18905"
                     /product="CYTH and CHAD domain-containing protein"
                     /protein_id="WP_182893135.1"
                     /transl_table=11
                     /translation="MRSAESGRKYELSLKARVPRLAGEGVTQEAPAERTLETVYYDTKD
                     FRLTRAGIALRRNSGTGWSLKLPSQELRFPLGDSPKAPAQIATLLRAYTLDRKLRPVAQ
                     LRTDRFTHRLTDASGRTLATLADDHVTGEFLGGPAVRLDRWRELDVEAGPELLDRLDRT
                     LRKRGAENAWWPSKLQRLMGDAAENKRGSNEVLPAYLRAQFERLRRADLGFRVGEDDSV
                     HQLRVAARKLRSALQTFASLVGRKKADAVTAELKWLGAELAPARDAEVSQARLEACLDE
                     VSPELVFGPLRQYLTRDFARTSRTELARATEALSSNRYAALLRSIAALLDTMRPAGKKA
                     LRKPLRKTARKLCRATAATEGLSGVELEKALHNVRKKAKRARYAADAVRPAFGKKLRAW
                     RKHVKAVQQTLGKHQDSVVDRAALRHFAIDGFSENQNTFTFGLLYGRDEAAAKMLQGRF
                     ADEWRTLRKGARPSWLKV"
     gene            complement(3586..4710)
                     /locus_tag="L3Q65_RS18910"
                     /old_locus_tag="L3Q65_18910"
     CDS             complement(3586..4710)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009080018.1"
                     /locus_tag="L3Q65_RS18910"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L3Q65_18910"
                     /product="NADP-dependent malic enzyme"
                     /protein_id="WP_182893136.1"
                     /transl_table=11
                     /translation="MSSRGKIQVTTRVRLDDADDLAEHYTPGVAELAERVAEDPSVLAR
                     ETVRGNSVAIVSDGSALLGLGDQGPAAALPVMEGKAALLKRFADVDAWPICPVSREPED
                     LVRTAKDLATSFGAINLEDIAAPRCFTVERQLHDELDVPVFHDDQHGTAVAVLAALHNA
                     LKLTDRTPAATSVVISGAGAAGSAIARLLLKADFSAERIVVCDSKGTLHGERDLTGEKQ
                     WLAENTNRGDAVQGSIQDALHDADVFVGVSVGGLLKGGDLARMADRAIVFALANPDPEV
                     DPDAAHEHAEIVATGRSDLPNQINNVLVFPGMFRGLLDSGAPRVTARMLLAASHALAAT
                     VGDDLDREHIVPSVFDEGLVPAIAEGVAGAAEND"
     misc_feature    complement(4869..4882)
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: weak, score: 18.26"
                     /tool="antismash"
     misc_feature    4873..4887
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.8"
                     /tool="antismash"
     gene            complement(5001..5813)
                     /locus_tag="L3Q65_RS18915"
                     /old_locus_tag="L3Q65_18915"
     CDS             complement(5001..5813)
                     /GO_component="GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Linocin_M18"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009080020.1"
                     /locus_tag="L3Q65_RS18915"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L3Q65_18915"
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="WP_182893137.1"
                     /sec_met_domain="Linocin_M18 (E-value: 1.4e-76, bitscore:
                     256.1, seeds: 61, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNHLMRDLAPIPAEGWKQIDDEARERLGTHLAARKMVDVEGPHGW
                     THSATPLGRTRRVDPPEAARERETLAQQRRVLPLVEVRVPFTVQRKELEDAERGADDLE
                     FDDLDQAAAQVGLLENRAVFHGWPDAGITGIAEASPYDHGTLGSDPERYPHIVANAVNT
                     LRCNGIEGPYALAINPGGYTSIVESTEHGGLLVLDHLRRALGGGRVKRTPGLTGAVVLS
                     LSGGDFVLELGQDLSVGYRHHDAETLTLYLEESFSFRVTEPDAALVLD"
     gene            complement(5813..6373)
                     /locus_tag="L3Q65_RS18920"
                     /old_locus_tag="L3Q65_18920"
     CDS             complement(5813..6373)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675229.1"
                     /locus_tag="L3Q65_RS18920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L3Q65_18920"
                     /product="hemerythrin domain-containing protein"
                     /protein_id="WP_182893138.1"
                     /transl_table=11
                     /translation="MTDITSLILDDHERFRRAFADLDDLDGPDELARAWEPLADLLDLH
                     AAAEEAVFYPELIQRGTDAEDETLDAVGDHNDIRDAVAEARRHPAGSAAWHAAVRQART
                     ANSEHMAEEEDDALADFRKHADPGLREELGRKFLEFKAEHEGGKGIDTGDVDPERYVEE
                     QERKAGKSSSGDGSLGIGSLKGE"
     misc_feature    6342..6355
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 18.28"
                     /tool="antismash"
     gene            6451..7068
                     /locus_tag="L3Q65_RS18925"
                     /old_locus_tag="L3Q65_18925"
     CDS             6451..7068
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009080024.1"
                     /locus_tag="L3Q65_RS18925"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears to
                     promote DNA repair by a pathway involving RecA, rather than
                     base excision; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /old_locus_tag="L3Q65_18925"
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="WP_182893139.1"
                     /transl_table=11
                     /translation="MVARQEPGAEPPDTVSLDKLRRAAGDCRGCGLYRDATATVFGAGA
                     ERADIFALGEQPGDQEDRRGEPFVGPAGRLLDKALGDAGLDRGSLYLTNAVKHFKFKLR
                     GKRRIHEKPARSEVVACRPWLVAELRAVQPRLVLLLGATAAQSVYGSSFRLTRHRGEPL
                     DPPEEFAGLFASAVATVHPSAVLRAPDRDTAYDEFVADLRGL"
     gene            7104..8294
                     /locus_tag="L3Q65_RS18930"
                     /old_locus_tag="L3Q65_18930"
     CDS             7104..8294
                     /EC_number="1.1.1.-"
                     /GO_function="GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA]"
                     /GO_function="GO:0008270 - zinc ion binding [Evidence IEA]"
                     /GO_function="GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_zinc_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1040: alcohol dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009080025.1"
                     /locus_tag="L3Q65_RS18930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L3Q65_18930"
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="WP_237600437.1"
                     /sec_met_domain="ADH_N (E-value: 1.5e-28, bitscore: 97.9,
                     seeds: 66, tool: rule-based-clusters)"
                     /sec_met_domain="ADH_zinc_N (E-value: 4.2e-13, bitscore:
                     48.5, seeds: 86, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKAVTWHGKRDVRVEAVPDPKIEEPTDAVVRVTSTGICGSDLHLY
                     EVLGPFMTEGDILGHEPMGIVEEVGSGVSSLKPGDRVVVPFNISCGHCWMCERGLQSQC
                     ETTQVKDQDKGAALFGYTKLYGQVPGGQAEYLRVPQAQYGPIKVPDGPPDERFVYLSDV
                     VPTAWQAVEYADIPDGGSVVVFGLGPIGQMAARVAQHQGAGEVIGVDLVPERLARARAH
                     GATTLDLRDHRQIGDAIRDLTGGRGADSVIDAVGMEAHGAPIGKLAHNLVALLPQPLGA
                     KITEKAGIDRLSVLYAAIDSVRRGGTISLSGVYGGMADPIPMMEVFDKQIRLHTGQANV
                     RRWIDDVLPVLTADGDPLGVEGFATHKLPLADAPRAYEMFQKKLDGVQKILLEPAA"
     misc_feature    complement(7113..7123)
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 18.59"
                     /tool="antismash"
     gene            complement(8326..9273)
                     /gene="nrfD"
                     /locus_tag="L3Q65_RS18935"
                     /old_locus_tag="L3Q65_18935"
     CDS             complement(8326..9273)
                     /codon_start=1
                     /gene="nrfD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674544.1"
                     /locus_tag="L3Q65_RS18935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L3Q65_18935"
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="WP_182893141.1"
                     /transl_table=11
                     /translation="MSPRRERAMVEPAEFRSYYGRPILKEPAWKQPDVPLYLFLGGAAG
                     ASASLAALADATGRPELAKAGRLAASGGSIASVVALIHDLGKPARFLNMLRVLKPTSPL
                     SVGSWILSPFSGLAAGAAFSTLTGRFPRLGRLAGAGAGVLGPAMCTYTAVLLADTATPS
                     WHEAHGTLPVLFAGSALTSGAGIALLAVPRKENGPAVRAGLAGAAAELAAEHHLETGLG
                     LASEPYRTGRAGRLLKAAKALTAAGAALSLAARKNRAAGVAAGAAYLASGLCTRFGVYA
                     AGVESAKDPKYVVVPQRERLAQREAVREGPLEGS"
     gene            complement(9270..10103)
                     /locus_tag="L3Q65_RS18940"
                     /old_locus_tag="L3Q65_18940"
     CDS             complement(9270..10103)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009080028.1"
                     /locus_tag="L3Q65_RS18940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L3Q65_18940"
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="WP_182893142.1"
                     /transl_table=11
                     /translation="MAEYGAQPRMGFFTDTSVCIGCKACEVACKEWNGVPEAESAPDLL
                     GLSYDNTGDLGSNTWRHVAFIEQPAVDLGMPTVGAPEQQQPEEAGVRWLMSSDVCKHCT
                     HAACLDVCPTGALFRTEFDTVVVQQDICNGCGYCVPACPYGVIEKRESDGRAFKCTLCY
                     DRLGAGMEPACAKACPTDSIQFGELDELRERAAARVSALHDDGVPEARLYGHDPGDGVG
                     GDGAFFLLLDEPEVYGFPPDPVVTTRDLPRMWKRAATTAAGVAAALVVSFLGRRR"
     misc_feature    complement(10637..10652)
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 20.05"
                     /tool="antismash"
     misc_feature    10646..10664
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 23.02"
                     /tool="antismash"
ORIGIN
        1 gtcagcgagt cgcagtggca gcctcggtga gctgctcccg caaggtgctc agcgtccggg
       61 ccagcagccg cgaaacctgc atctgcgaca gcccgacccg atcggcgatc tggctctgcg
      121 tcatgttcga gaagaaccgc atgacgacga tcgcgcgctc ccgctccggc aggctccgca
      181 gcagcggctt gagcgtctcc cggtcctcga gcttctcgat ttccgggtcc tcgtcgccga
      241 gggtgtcggc cagcgcgagc gagccggtct cgtcgtgggc cgggtagtcg atggacgcgg
      301 cctggtaggc gttcccggcg tgcagggctt cggtcacctc gtccaggtcg aggtcgagat
      361 ggtcggcgag ttcgcgcgcg gtgggcgcgc ggccgatgcg ctggcccagg tcggtggcgg
      421 cctggccgat cgtcacgtgg agttccttga gccgccgcgg gacgcggacc gaccaggtgt
      481 ggtcgcggaa gtagcggcgg acctcgccca tcacggtcgg gactgcgaag gcgacgaaat
      541 ccgtctgccg cgccggatcg tagcggtcga cggcgttgat gaggccgatc cgcgccacct
      601 gcaagaggtc ctcggacggc tggccgcgcc gggcgaagcg gcgcgcgatg tgctcggcca
      661 gcggaaggtg ctcctcgatg aggcggcggc gcagccgcga gtgctcgtcg gtgtcctcgg
      721 cgaggtggct gatctcctcg aacagcaccg cgcagtggcg gtagtcgtcg cgccgggtgc
      781 gggtcatggt cgccgcggtc atgtaaatcc tcctgacagc cgaagggaac gtgactcgtg
      841 tagtgccatg gagcgcatcg cgttgccgac gctaatcatc cttagtggac agtgcccggc
      901 gtcgcaaggc gagcgcgcgg cggatccgcg tgctggccgg ggcgacaggc agccggagtg
      961 tcatggccgg gtggtacccg cccgaggaca ggatcaatcg gccggccacc cgaatggggg
     1021 ctcaagtcac atcgcgcaga cgcagcagag tgtcgagttc ggcggaactg ccgacggaac
     1081 gcaggacggg cggcgcgagc tgcccgatgc ggtgatcgcc gcgcgcaatg cggtgacgaa
     1141 ccggaggagc tgcgcgggcg gcggaacgac ggtggcgccg ccgaaatagc ggagaacgat
     1201 gagatcttca ccggaatgcg tttcgaccgc ggtcgaccat ccgtggacct cgtcccacag
     1261 caacgcgaca tcccgcccgg ggcaacccgg cagatgaccg tcgagagcga cgtacgccga
     1321 caccggcggg cctgggtcga gagcgcagaa ctccggaccg accccgagcg cccgcgcgac
     1381 ctcggcgaga tagccccgca gcggggcgag gacggcgtgc ggattgccgg gcgggacgtg
     1441 cggcgtcgcg atcacgggcg cctttccggc ggaaggatca gcttccgggc cgccagcgcc
     1501 cgcgcaccga acggcggtcg gcgcggctga gcacgcccga ggtgtcgcgc gtccagtggc
     1561 cgccgcggga atcggtgaag gacaggccgg gcacggcggc cgcggagtac aggtcgtcca
     1621 ccggcatggc gtcctcgccg aacggcagtg cccggacggc agtggcgccg ggcgggatga
     1681 tcgcgaaccg cgcacagtgc ggctcctcct cgtccgggcg gctgacctcc agcgaacagt
     1741 cgaacaccgg tgcgtcgccg ctgttgtgcg cgcagacgac gtagtcctcg tcccggtcgt
     1801 gccgggcgcc ctcgtcgagc cacgcggaga ccgactgcgc ctgctgctcc tgccggactt
     1861 ccgcctggtc tttccggttg agcagcatga tccacacata cgaggcgaac cccagcagcg
     1921 agcccgcgct cccgatcgcc gacacccacg tcgcggcaag ttgtgatccc atttccggcg
     1981 gggttccccg atccggccgc ggccaaaccc ggcccggcag aggtttagac ctccgccggc
     2041 cggggcagca ataacccgcc cccggacttt cgttgccgcg gggacgccgg cgatgccgag
     2101 acatcgccgg cttttctgct gtccacagtg gacttccggg ggctcagacc ttcagccagg
     2161 acggtcgagc gcccttgcgc agcgtgcgcc actcgtcggc gaacctgccc tggagcatct
     2221 tcgccgcagc ctcgtcccgg ccgtagagaa gaccgaaggt gaacgtgttc tggttctcgc
     2281 tgaaaccgtc gatcgcgaaa tgccgcagcg ccgcacggtc gacgacgctg tcctgatgct
     2341 tgcccagtgt ctgctgcacc gccttcacgt gcttgcgcca cgcgcgcagc ttcttgccga
     2401 acgctggccg gacggcgtcg gcggcataac gtgcgcgctt ggccttcttc cggacgttgt
     2461 gcagtgcctt ctccagttca acgccactga gcccttccgt cgccgcagtg gcccggcaga
     2521 gtttgcgcgc ggtcttgcgc aggggtttgc gcagcgcttt cttgcctgcc ggacgcatcg
     2581 tgtcgagaag ggcggcgatc gaccgcaaca gggccgcgta tcggttgctg gacaacgctt
     2641 ccgtcgcgcg ggccaattca gtgcgggagg tgcgggcgaa gtcgcgggtc aggtattggc
     2701 gcaggggacc gaaaaccagc tccggcgaga cctcgtcgag gcaggcttcg aggcgcgctt
     2761 ggctgacctc ggcgtcccgc gcgggggcga gttcggcgcc cagccacttc agctccgccg
     2821 tgaccgcatc ggctttcttc cggcccacca gagacgcgaa cgtctggagc gcgctccgca
     2881 gcttccgcgc cgcgacccgg agctggtgca ctgaatcgtc ttccccgacg cggaaaccca
     2941 agtcagcgcg acgcaatcgc tcgaattgcg cgcgcagata cgcaggcagc acctcgttcg
     3001 agccccgctt gttttcggcg gcgtctccca tcagccgctg cagtttcgac ggccaccaag
     3061 cgttctccgc cccgcgcttg cgcaaggtgc ggtcaaggcg gtcgagcagc tcaggtcccg
     3121 cctcgacgtc cagttcgcgc caccggtcca gccgcaccgc cggtccccca aggaattctc
     3181 cggtgacgtg gtcgtccgcg agcgtcgcca gcgttcggcc ggacgcgtcg gtcaggcggt
     3241 gcgtgaaccg gtcggtccgc agctgcgcga cggggcgcag cttgcggtcc aatgtgtatg
     3301 cacggagcag cgttgcgatt tgagcgggcg ccttcgggct gtcaccgagc ggaaatcgca
     3361 gctcctggct gggcaatttc agggaccacc cggtgccgct gtttcttcgc agcgcgatcc
     3421 cggctctggt cagccggaaa tccttggtgt cgtagtaaac ggtctcgagc gtccgctcgg
     3481 ccggagcttc ctgtgtgact ccctcgcccg ccagccgcgg cacgcgggct ttcaagctca
     3541 gctcgtactt gcggccggac tctgcgcttc gcattccccg ctccttcagt cgttttcggc
     3601 ggcgccggcg acgccttcgg cgatcgcggg caccaggccc tcgtcgaaga cgctcggcac
     3661 gatgtgttct cggtcgaggt cgtcgccgac cgtcgcggcc agcgcgtgcg aggccgcgag
     3721 caacatccgt gcggtcaccc tcggagcgcc ggagtcgagc agcccgcgga acatccccgg
     3781 gaagacgagg acgttgttga tctggttggg caggtcgctg cgtccggtcg cgacgatctc
     3841 cgcgtgctcg tgcgcggcgt ccggatcgac ctccggatcc gggttggcca gcgcgaacac
     3901 gatcgcccgg tcggccatcc gcgccaggtc gccgcccttg agcagaccgc cgacgctcac
     3961 gccgacgaag acgtccgcgt cgtgcaaggc gtcttgaata cttccctgga cggcgtcgcc
     4021 gcggttggtg ttctccgcca gccactgctt ctcgccggtc aggtcgcgct cgccgtgcag
     4081 agtgcctttg ctgtcgcaga ctacgatccg ctccgcgctg aagtcggcct tcagcagcag
     4141 ccgggcgatc gcggacccag ccgcgcccgc accggagatc accaccgacg tggctgccgg
     4201 cgtgcggtcg gtcagcttca gcgcgttgtg cagagcggcc agcacggcta ccgcggtgcc
     4261 gtgctggtcg tcgtgaaaga ccggcacgtc cagctcgtcg tgcagctgcc gctcgacggt
     4321 gaagcaccga ggcgcggcga tgtcctccag gttgatcgcg ccgaaggacg tcgcgaggtc
     4381 cttcgcggtg cgcaccaggt cttccggctc gcggctgacc gggcagatcg gccaggcgtc
     4441 gacgtcggcg aaccgcttca gcaacgccgc tttgccctcc atcaccggca gcgcggcggc
     4501 cgggccctgg tcgccgagcc cgagcagcgc ggaaccgtcg gagacgatcg cgacggagtt
     4561 gccgcgcacg gtttcccgcg ccagcaccga cggatcctcg gcgacgcgct cggccagctc
     4621 ggcgacgccg ggcgtgtagt gctcggcgag gtcgtcggcg tcgtcgagcc gcactcgggt
     4681 ggtgacttgg atctttccgc gactgctcat gcgagccgga tacccggccg cgccgcggcg
     4741 gaatcgtgct cagccggagc tccgcccgct gcccctctcc cctccgctga cgggcagatg
     4801 tccgtgaggg gaacccggag aaccccaatg ccacattggg tgcgtcgcag atacggtccg
     4861 gtgagcatca accgagtgtt tcggccaggt cggcgtcctg tgcggcggtc agccgggcga
     4921 cgcggctcaa ggtcccggcg tggggactca gagtcgctca cggctcgctg ccgcggtgcc
     4981 cacacccggc gccggcgtgc tcagtccagc acgagcgccg cgtccggttc ggtcacccgg
     5041 aaactgaagc tttcctccag gtacagcgtg agcgtctcgg cgtcgtggtg gcggtagccg
     5101 accgagaggt cctggccgag ctccagcacg aagtccccgc cggacaggct cagcacgacc
     5161 gcaccggtca gccccggcgt gcgcttcacc cggccgccgc ccaacgcccg gcggaggtgg
     5221 tcgagcacga gaagaccgcc gtgttcggtg ctctccacaa tggaggtgta gccgccgggg
     5281 ttgatcgcga gcgcgtacgg tccttcgatt ccgttgcacc gcaatgtatt caccgcgttg
     5341 gccacgatat gcggatagcg ttccggatcg ctgccgaggg tcccgtggtc gtacgggctc
     5401 gcctcggcga tcccggtgat cccggcgtcc ggccagccgt ggaacaccgc gcggttctcc
     5461 agcaggccca cctgggcggc ggcctggtcg aggtcgtcga actccaggtc gtcggcgccg
     5521 cgctcggcgt cctcgagctc cttgcgctgg acggtgaacg gcacccgcac ctccaccagc
     5581 ggcaacaccc ggcgctgctg cgcgagggtt tcgcgctccc gggcggcttc aggcggatcg
     5641 acccgccggg tccggccgag cggcgtggcc gagtgcgtcc agccgtgcgg gccttcgacg
     5701 tcgaccatct tgcgcgccgc cagatgcgtc cccagccgct cccgcgcctc gtcgtcgatc
     5761 tgcttccagc cctcggccgg gatcggcgcg aggtcgcgca tcaggtggtt catcattctc
     5821 ccttcaggct gccgatcccc agcgatccgt cgcccgacga gcttttcccg gctttccgct
     5881 cctgctcctc gacgtaacgc tccgggtcga cgtccccggt gtcgatgccc ttcccgccct
     5941 cgtgctcggc cttgaactcg aggaatttcc ggccgagttc ctcgcgcagg cccggatcgg
     6001 cgtgcttgcg gaagtcggcg agcgcgtcgt cctcctcctc ggccatgtgc tcgctgttgg
     6061 cggtgcgggc ctgccggacc gccgcgtgcc aggccgcgct gcccgccgga tgcctgcggg
     6121 cttcggcgac ggcgtcgcgg atgtcgttgt ggtcgccgac cgcgtcgagc gtctcgtcct
     6181 cggcgtccgt gccgcgctgg atcagctcgg ggtagaagac cgcctcctcg gccgcggcat
     6241 gcaggtccag caggtccgcg agcggttccc acgcccgcgc cagctcgtcc gggccgtcga
     6301 ggtcgtcgag gtcggcgaac gcccgccgga accgctcgtg atcgtcgagg atcaagctgg
     6361 tgatgtccgt catggccctc gacgttagct ctcccggcgg cggcgcgctc gtcgtgtttg
     6421 cgcgaggctg cttgcgggta aagagatccg atggtcgcgc gtcaggaacc gggagccgaa
     6481 ccgccggaca ccgtcagcct cgacaagctc cgccgagccg ctggcgattg ccgcggctgc
     6541 gggctttacc gcgacgccac ggcgacggtt ttcggcgccg gggccgagcg tgcggacatc
     6601 ttcgctctcg gagagcagcc cggcgaccag gaggaccggc gaggcgaacc gttcgtcggc
     6661 ccggccgggc gcctgctcga caaggcgctc ggcgacgcgg gcctcgaccg cggttccctc
     6721 tacctgacca acgcggtcaa gcacttcaag ttcaagctcc gcggcaaacg ccggatccac
     6781 gaaaaaccgg cgcgctccga ggtcgtcgcg tgccgtccgt ggctggtggc cgaacttcgg
     6841 gcggtacagc cccgtctcgt gctgttgctg ggcgcgacgg cggcgcaatc ggtgtacggc
     6901 agcagtttcc ggctcacccg gcatcgcggc gaaccgctgg atccgccgga agagttcgcc
     6961 ggcctgttcg cctcggcagt cgccaccgtg cacccgtccg ccgtgctgcg cgcgcccgac
     7021 cgcgacaccg cgtacgacga gttcgtcgcc gacctgcgcg gcctctgacg tttgcccgtc
     7081 ccggcaaggg gaatccggcc gccatgaaag cagtgacctg gcacggcaag cgggacgtgc
     7141 gagtcgaggc cgtcccggac ccgaaaatcg aggagccgac cgacgcggtc gtgcgggtga
     7201 cctcgaccgg catttgcggt tccgacctgc acctgtacga ggtccttggc ccgttcatga
     7261 ccgagggcga catcctcggc cacgagccga tggggatcgt cgaggaagtg ggcagcggcg
     7321 tgagttcgct gaaacccggc gaccgggtgg tggtgccgtt caacatttcc tgcggacact
     7381 gctggatgtg cgagcgcggg ctgcaatcgc agtgcgagac cacccaggtc aaggaccagg
     7441 acaagggcgc ggcgctgttc ggctacacga agctctacgg ccaggtgccc ggcggacagg
     7501 ccgagtacct ccgggtcccg caggcgcagt acgggccgat caaggtgccc gacggcccgc
     7561 cggacgaacg gttcgtctac ctgtccgatg tggtcccgac ggcgtggcag gccgtggaat
     7621 acgcggacat ccccgacggc ggctcggtcg tggtcttcgg tctcggcccg atcggccaga
     7681 tggcggccag ggtcgcgcag caccagggcg cgggcgaggt gatcggcgtc gacctcgtgc
     7741 cggaacggct ggcgcgggcc cgcgcacacg gcgcgacgac gctggacctg cgcgaccacc
     7801 ggcagatcgg cgacgcgatc cgcgacctca ccggcggccg cggcgccgat tcggtgatcg
     7861 acgcggtcgg catggaagca cacggcgcgc cgatcgggaa gctcgcgcac aacctggtcg
     7921 cgctgctgcc gcagccgctc ggcgcgaaga tcaccgagaa ggccggaatc gaccggctga
     7981 gcgtgctgta cgcggccatc gacagcgtcc ggcgcggcgg cacgatctcg ctgtccggcg
     8041 tgtacggcgg gatggccgac cccatcccga tgatggaagt gttcgacaag cagatccggc
     8101 tgcacaccgg acaggcgaac gtccgccggt ggatcgacga cgtgctgccg gtgctcaccg
     8161 cggacgggga tccgctcggc gtcgaaggct tcgcgacgca caagcttccg ctggccgacg
     8221 cgccgcgcgc ctacgagatg ttccagaaga aactcgacgg ggtgcagaaa atcctgctcg
     8281 agccggcggc atagccagcg gtccgtgaag gactccttga gggaatcagc ttccctcaag
     8341 aggcccttca cggaccgctt cgcgctgcgc gagccgttcc cgctgcggaa ccacgacgta
     8401 cttcgggtcc ttcgcggact ccaccccggc cgcatacacg ccgaaccgcg tgcacaatcc
     8461 ggaggcgaga taagccgccc cggcagcaac gcccgccgcg cggttcttcc gtgctgccaa
     8521 cgacaaagcc gctcccgccg cggtcagcgc cttggccgcc ttcagcaatc gcccggcccg
     8581 cccggtccgg taaggctccg aagccagccc cagcccggtc tccaggtgat gctcggcagc
     8641 cagctccgcc gcggcaccag ctagcccagc ccgcaccgcg ggaccgtttt ccttccgcgg
     8701 caccgcgagc agcgcgatcc ccgccccgct ggtcagcgcg ctgcccgcga acagcaccgg
     8761 cagcgtgccg tgcgcctcgt gccacgacgg cgtcgccgtg tcggccagca gcaccgccgt
     8821 gtacgtgcac atcgccgggc ccagtacgcc cgcgcccgcc ccggccaacc ggcccagccg
     8881 cgggaaacgt ccggtcagcg tggaaaacgc cgcccccgcg gcgagaccgg agaacggcga
     8941 caggatccac gaccccaccg acaacggcga agtcggcttg agcacgcgca gcatgttcag
     9001 gaaccgcgcc ggcttgccga ggtcgtggat cagcgcgacg acgctggcga tcgatccgcc
     9061 ggaagccgcc agccggccgg ccttggccag ttccgggcgt ccggtcgcgt ccgcgagggc
     9121 ggccagcgac gccgacgcgc cggccgcgcc gccgaggaac aggtacagcg ggacgtccgg
     9181 ctgcttccag gcgggctctt tgaggatcgg cctgccgtaa taagaccgga attcggcggg
     9241 ctcgaccatc gcccgttcgc ggcgggggct catcggcgcc gtcccaggaa cgacacgacc
     9301 agcgccgcgg ccaccccggc cgcggtcgtc gcggcgcgct tccacatccg cggcaggtcg
     9361 cgggtcgtca cgaccgggtc cggcgggaag ccgtagacct caggttcgtc cagcagcagg
     9421 aagaacgcgc cgtcgccgcc gaccccgtcg ccgggatcgt gcccgtagag ccgcgcctcc
     9481 ggcacgccgt cgtcgtgcag cgccgacacg cgcgcggcgg cgcgttcgcg gagttcgtcc
     9541 agctcgccga actggatgga atcggtcggg caagccttgg cgcacgcggg ttccatcccc
     9601 gcgccgagcc ggtcgtaaca aagggtgcac ttgaacgcac ggccgtcgct ctcgcgcttc
     9661 tcgatgaccc cgtacgggca agccggaacg cagtagccgc agccgttgca gatgtcctgc
     9721 tgcaccacga ccgtgtcgaa ttcggtgcgg aacagcgcgc cggtcgggca gacgtcgagg
     9781 caggcggcgt gcgtgcagtg cttgcagacg tcgctggaca tcagccagcg cacgcctgct
     9841 tcttccggct gctgctgctc cggcgctccg accgtcggca tccccaggtc gaccgcgggc
     9901 tgttcgatga acgccacgtg ccgccaggtg ttcgagccga ggtcgccggt gttgtcgtag
     9961 gacagcccga gcaggtccgg cgcgctttcg gcctccggga cgccgttcca ctccttgcac
    10021 gcgacctcgc acgccttgca cccgatgcac accgacgtgt cggtgaaaaa gcccatccgg
    10081 ggctgggcgc cgtactcagc cattggctcg cctccggtac tccgccacga actccagcag
    10141 cgccggcccg cgcggccgcc gtccgggacg gatgtcgcag gtggccgctt tggcctcctg
    10201 gatgtgcacg ttcgggtcca gcacgatcga cagcaggtcg ttggccgcgt cgccgcggga
    10261 cagtccgttc gggccccagt ggtagggcag cccgacctga tggacggtgc ggccgcgcac
    10321 cttgagcggt ttgacgcggt cggtgaccag cacgcgggcc tcgatcgccg tccgcgccga
    10381 cacgacagtg gcccagccga ggtgcaccag cccgcgttcg gcggccagcg cgggcgagat
    10441 ctcgcagaag aactccggct gcagttcgga caggtacggc agcgtccggc tcatcccgcc
    10501 cgccgtgtgg tgctcggtga gccggtaggt ggtgaacacg tacgggtaga cctcgctgcg
    10561 cgccgggttg tactggttca ccgggctgtc cagcgtctgc cgcaccggcg aggactgctg
    10621 gccgtagagg gcgttgccga ccgggctttc gaacggctcg taatgcgtgg gcagcggacc
    10681 gtcggccagc ccggcgggga cgtacagcca ggctttgccg tcggtctgca tgatgaacgg
    10741 gtcgcggccg ctcagcgcgt cctgcgcctt cgccccgtcc ggcggctggt aatcgggtgg
    10801 cttggcggcc tcg
//
