LOCUS       NZ_CP091381            10804 bp    DNA     circular CON 18-AUG-2024
DEFINITION  Amycolatopsis sp. FU40 chromosome, complete genome.
ACCESSION   NZ_CP091381
VERSION     NZ_CP091381.1
KEYWORDS    RefSeq.
SOURCE      Amycolatopsis sp. FU40
  ORGANISM  Amycolatopsis sp. FU40
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Amycolatopsis.
REFERENCE   1  (bases 1 to 9944196)
  AUTHORS   Reisman,B.J. and Bachmann,B.O.
  TITLE     Biosynthesis of Apoptolidin Family Glycomacrolides
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9944196)
  AUTHORS   Reisman,B.J. and Bachmann,B.O.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2022) Chemistry, Vanderbilt University, 1234
            Stevenson Center Drive; Room 7330, Nashville, TN 37235, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 23-JAN-2022
            Assembly Method                   :: Flye v. 2.9
            Assembly Name                     :: NFU40_flye_2.9
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 47.0x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_021903635.1-RS_2024_08_18
            Annotation Date                   :: 08/18/2024 04:30:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,491
            CDSs (total)                      :: 9,417
            Genes (coding)                    :: 9,360
            CDSs (with protein)               :: 9,360
            Genes (RNA)                       :: 74
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 7 of 57
            Pseudo Genes (incomplete)         :: 50 of 57
            Pseudo Genes (internal stop)      :: 5 of 57
            Pseudo Genes (multiple problems)  :: 5 of 57
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:13:09
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 459713
            Orig. end                         :: 470517
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP091381.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Brian O. Bachmann,
            Vanderbilt University.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10804
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="2"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10804
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10804
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5804](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5804)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            23..1432
                     /gene="hemG"
                     /locus_tag="L3Q65_RS03380"
                     /old_locus_tag="L3Q65_03380"
     CDS             23..1432
                     /EC_number="1.3.3.4"
                     /GO_function="GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA]"
                     /GO_process="GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="hemG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013224513.1"
                     /locus_tag="L3Q65_RS03380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L3Q65_03380"
                     /product="protoporphyrinogen oxidase"
                     /protein_id="WP_237598767.1"
                     /transl_table=11
                     /translation="MKHVAVVGGGISGLAAAYRVRRLLGDRVTITVFESTASIGGKLRT
                     AEVGGVSYDVGAEAFLARRPEVVSLVEEVGLADRLVHPTKARAKIHAGGSVQSLPPGTV
                     MGVPASADSVAGVLSEDGRSAVEKERSLGPVDLGEGDVPLGPLLRTRFGDELVDRLVDP
                     LLGGVYAGGADGLGLRATMPGLANAIEGGAGSLTEAASSLLPKTPGTAPVFGSLTGGLR
                     GLLDRLLELSGAELRTSSTVRALTRTPSGWRLEFGAAAPAHAPADSPVDADAVLLAVPA
                     PAARKLLDGVAPAASTAFGEVELASMAVVAFAFPPGTELPDASGILIGAGERDAEGVPY
                     AAKAFTFSSNKWAHLGDAGPVLVRGSVGRFGEPGALNGDDDSLVEAVRADLARLAGVTA
                     KPIDTLVTRWGGGLPQYGTGHLERVTRIETAVADVPGLAVAGATLHGVGLPACVATADA
                     AARRIAEQLFV"
     gene            1479..2171
                     /gene="hemQ"
                     /locus_tag="L3Q65_RS03385"
                     /old_locus_tag="L3Q65_03385"
     CDS             1479..2171
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017983668.1"
                     /locus_tag="L3Q65_RS03385"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L3Q65_03385"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_182890512.1"
                     /transl_table=11
                     /translation="MARLNYHELNDTIRYTTWSVFRIEPGKLGEDRGAAGRETAEYLDG
                     LEAKGVVVRGVYDVSGLRADADYMIWWHAEEIEQVQAAYTGFRRTPLGRASTPVWSQTA
                     LHRPAEFNKSHVPAFLAGEEARKYICVYPFVRSYDWYLLPDEERRKMLADHGKEARDYP
                     DVRANTVASFALGDYEWILAFEADELHRIVDLMRHLRNTEARRHVRVEIPFYTGTKVPP
                     AELVAALP"
     gene            2178..3545
                     /locus_tag="L3Q65_RS03390"
                     /old_locus_tag="L3Q65_03390"
     CDS             2178..3545
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /GO_function="GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1189: L-carnitine dehydratase/bile acid-inducible"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009075645.1"
                     /locus_tag="L3Q65_RS03390"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L3Q65_03390"
                     /product="CoA transferase"
                     /protein_id="WP_237598768.1"
                     /transl_table=11
                     /translation="MQDVLAGAWQSLAGEPLPAVSLTGDESVLPGHFRVPAMASASIAA
                     ATLSAGELLRLRGIDPGTVSVDTRHAVAAFRSEHLLRIDGEPGPSPWGRIAGDYRAADG
                     WIKLHCNTSRHEAAVCWALGVPATREAVTKAVAGKTTAEVESAVVTAGGAAALMRSRED
                     WLAHPQGQAVAELPLVELNRIGDAPKRTLFDSDRPMGGVRVLELTHVLAGPVAGRVLAA
                     HGANVLHVGAAHLPRIPVLTMDTGQGKRSAFVALDTEGGRAKLKKLIARADVLLQTFRP
                     GALARIGFGPDELAELRPGLVIADVSAYGWTGPWARRRGFDSLVQMSSGIAEEYATDAP
                     APLPAQALDHATGWLTAAAIMTAIRRTATDGGTWHARLSLAGTGRWLDSLGRKDPEPSE
                     ADYADVLETVDSDFGRMTRVRMAGELPGAPPHWDHPSHRPGADTAVWSPVVPQPVS"
     gene            complement(3797..4576)
                     /locus_tag="L3Q65_RS03395"
                     /old_locus_tag="L3Q65_03395"
     CDS             complement(3797..4576)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR04222.1"
                     /locus_tag="L3Q65_RS03395"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L3Q65_03395"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_182890510.1"
                     /transl_table=11
                     /translation="MNGFWAALSPMLLVYVATAAGLASILATGKDKLATQASAPEPAAV
                     PRDVYDVAYLAGGRPRLVEAAIATLLARGQLRVGSLGEIVAAGPPPASPPLEQAVHQAA
                     GRGRLASWVARSSVLKPVIRTLETDLVQRRLVSRRRPLTGLGYTLTAVALVVLAAGVGR
                     LVYDVGESHLNAMVLLVFGEVVLTLWLCLVGSQRITKTFAERKVSSLPPTTAGATVLAR
                     AQQQAGAGGSAGPAELVALGGLAAYPDKRVTGGLMPI"
     misc_feature    complement(4613..4630)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 17.71"
                     /tool="antismash"
     gene            complement(4678..5004)
                     /locus_tag="L3Q65_RS03400"
                     /old_locus_tag="L3Q65_03400"
     CDS             complement(4678..5004)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020658034.1"
                     /locus_tag="L3Q65_RS03400"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L3Q65_03400"
                     /product="hypothetical protein"
                     /protein_id="WP_182890509.1"
                     /transl_table=11
                     /translation="MSREELAARQAALLDSLLTGAEAPSGFDASRLRIEADVLLAKRRR
                     LVAYLRPDLPEALGPRYRPLFDTYAAERPKTTSIRAREYADAFAEWLIARGDLPKPRRR
                     WFRR"
     gene            complement(5001..5804)
                     /locus_tag="L3Q65_RS03405"
                     /old_locus_tag="L3Q65_03405"
     CDS             complement(5001..5804)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020658035.1"
                     /locus_tag="L3Q65_RS03405"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L3Q65_03405"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_182890508.1"
                     /sec_met_domain="DUF692 (E-value: 4.7e-83, bitscore: 277.6,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MADRLGIGIGWRPELDLSIARLPGVDWVEVVAENLHAPHLPESLL
                     ALKDRGMPVLPHAVSLSLGGAEPLDTARVEHLAELARALDAPLASDHVCFVRAGGLDSG
                     HLMPLPRSREALDVLVDNVRLAQSIVDVPLALENIAALLEWPENELSEEQFLTELTERT
                     GCLLIVDVANLYANARNVGTDPAAFLDGIPWERLAYVHMAGGVERDGVYHDTHAHPVLP
                     EVLELLAELRRRTDPPGVLLERDDDYPTDSELAAELVALRTAVTL"
     gene            complement(5797..6624)
                     /locus_tag="L3Q65_RS03410"
                     /old_locus_tag="L3Q65_03410"
     CDS             complement(5797..6624)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009075651.1"
                     /locus_tag="L3Q65_RS03410"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L3Q65_03410"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_182890507.1"
                     /transl_table=11
                     /translation="MHEPWGISGPQFLAIYGSALALTLVVQLGWPLLRKAPADVHRPLD
                     VYQLAYLAGGHHRTVDTAIAALLERGLLRVDSRGQLTAVSRTPTEPVEQAVHAAVRNRT
                     QTAATLRAKETCSVPVRNIRNELRERGLVTFASAGFATGVVLVYAALFVVGLVRSANGS
                     ALHRPTAGLNVLLAVTAILTVLAVTSRKRPKARLTKAGRGMVVAVRTDPAAQRAQSGAL
                     LVGAASLVALGGFAQYPDHATSLALRSGAGTSSGGGSSCGGGGGCGGGGGCGG"
     gene            complement(6672..7619)
                     /locus_tag="L3Q65_RS03415"
                     /old_locus_tag="L3Q65_03415"
     CDS             complement(6672..7619)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009075651.1"
                     /locus_tag="L3Q65_RS03415"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L3Q65_03415"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_237598769.1"
                     /transl_table=11
                     /translation="MDEPWGISGPQFLLIFGLALAVVLLVQISWPSIARSRRRSAPATV
                     EAPLQPDVYQLAYLAGGSDRTVDTAIATLLEQELLRVSSKGKLSAIGKRPARKRLERAV
                     HDAAQGGTATVAMVRKSPMVQPEVEKIRQDLERRGLLVAGDGLDGFRAGIVAGYAALLL
                     IGIVRAVNGAQLDRPIGGLIGLLVVAGTLTLIAYGVRKGSQTSRPTDAGHAIVVRARED
                     AKAAEERPEEFAATNRSGNGTLLVGAAAVVALGGIALYPDEEMSTALISGGGSWTGGGG
                     SSGGSSCSSGGSSCSSGSSCGGGSSCGGGGGCGG"
     gene            complement(7718..8626)
                     /locus_tag="L3Q65_RS03420"
                     /old_locus_tag="L3Q65_03420"
     CDS             complement(7718..8626)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR04222.1"
                     /locus_tag="L3Q65_RS03420"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L3Q65_03420"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_237598770.1"
                     /transl_table=11
                     /translation="MDWTIERAMPAALILVLALACWWLGRRTAARLNRGRHPETRLTSA
                     AQVALLTNGPIRVAETTVASMLEREQLRADSVGRLYRTPTAPSDALSREAAELTGTGVG
                     VATVLRGLVEGDTVRELIDDLTKRGLLVDGQALRRAWKWTAIEEAVLAAAGVAAFVGGL
                     NGYVLISVLLPAWFAFAALSRRRAARRIRPTDAGRTAAEAAWPDRSLISGLTGSVAVGG
                     LSNHPDRELRIALLRGLPATRRTGVAIAGAAGGFAGPAGYWAAGSGCASGCGGGAGCGS
                     GCGGSGCGGGCGGGGCGGGNS"
     gene            complement(8739..9167)
                     /gene="msrB"
                     /locus_tag="L3Q65_RS03425"
                     /old_locus_tag="L3Q65_03425"
     CDS             complement(8739..9167)
                     /EC_number="1.8.4.12"
                     /GO_function="GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /GO_process="GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /GO_process="GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /gene="msrB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009075655.1"
                     /locus_tag="L3Q65_RS03425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L3Q65_03425"
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="WP_182890504.1"
                     /transl_table=11
                     /translation="MKPVVGTTPRVVKSDQEWREQLAPEEYAVLRQAGTERPFTGEYTD
                     TKTTGVYECRACGAELFRSDTKFESHCGWPSFYDPADSDAVLLREDRTMGMRRIEVLCA
                     SCHSHLGHVFEGEGYATPTDQRYCINSISLKLVEDAGD"
     gene            complement(9187..9492)
                     /locus_tag="L3Q65_RS03430"
                     /old_locus_tag="L3Q65_03430"
     CDS             complement(9187..9492)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009075657.1"
                     /locus_tag="L3Q65_RS03430"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L3Q65_03430"
                     /product="hypothetical protein"
                     /protein_id="WP_116206679.1"
                     /transl_table=11
                     /translation="MAENGALPPGWNPRFHEDLVGLDPYDPEARAFAAHLDRMERTGPT
                     FTVEACLDQVADFADSSTRTGGLRYWVSALVVVLIVLGLLVTAWDIALHTVAFLSR"
     misc_feature    10617..10632
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 21.27"
                     /tool="antismash"
ORIGIN
        1 tcgtcgacct ggtgcactcg ctgtgaaaca cgtcgcggtc gtcggcggcg ggatctccgg
       61 gctggccgcg gcgtaccggg tgcgccggct gctcggcgac cgggtcacga tcaccgtctt
      121 cgagagcacg gcctcgatcg gcgggaagct gcgcacggcg gaggtcggcg gagtcagcta
      181 cgacgtcggg gccgaagcgt tcctcgcccg gcggcccgag gtggtttcgc tggtggagga
      241 ggtcggcctc gccgatcgcc tggtgcatcc gacgaaggcg cgcgccaaaa tccacgccgg
      301 cggttcggtg cagagcctgc cgcccggcac cgtgatggga gtcccggcgt ccgcggactc
      361 ggtcgcgggc gtgctgtccg aagacggccg cagcgcggtc gagaaagaac gttcgctcgg
      421 tccggtcgac ctgggcgaag gcgatgtgcc gctcgggccg ctgctgcgca cgcgcttcgg
      481 cgacgaactg gtggacaggc tggtcgaccc gttgctgggc ggcgtttacg cgggcggtgc
      541 cgacggtctc ggcctccgcg cgacgatgcc cggcctggcc aacgcgatcg agggcggagc
      601 gggttcgctc accgaggccg cgtcgtcgct gctgccgaaa acgccgggca ccgcaccggt
      661 tttcggcagt ctcaccggcg gcttgcgggg cttgctcgac cggttgctgg agctttccgg
      721 cgctgagctg cgcacttcgt caacggtccg cgcgctgacg cggacgccgt ccggctggcg
      781 tctcgaattc ggtgctgccg cgccagcgca cgcgcctgcc gacagcccgg tggacgcgga
      841 cgcggtgctg ctcgcagtgc ccgcaccggc cgcccggaaa ctgctcgacg gcgtagcccc
      901 ggcggcttcg accgcgttcg gcgaggtcga actggcgtcg atggcggtcg tcgcgttcgc
      961 gttcccgccc ggcacggagc tgcctgacgc gtccggaatc ctcatcggcg cgggggagcg
     1021 ggacgcggaa ggcgtgccgt acgcggccaa ggcgttcacg ttttcgtcga acaagtgggc
     1081 tcacctcggc gacgccggac cggtgctggt gcgcggttcg gtgggccggt tcggcgagcc
     1141 cggcgcgttg aacggcgacg acgattccct ggtcgaggcg gtccgcgcgg acctggctcg
     1201 cctcgccggc gtcaccgcga agccgattga cacgctggtg acccgctggg gcggcggcct
     1261 cccgcagtac ggcaccgggc acctggagcg ggtgacgcgg atcgagacgg cagtggcgga
     1321 tgtcccgggc cttgccgtgg cgggcgccac gctgcacggc gtcggcctgc cggcgtgcgt
     1381 ggccacggcc gatgccgctg cccgccggat cgcggagcag ctcttcgtgt gacggagccc
     1441 catggcgatc gccggaccgg aagtgccagg atggagtcat ggcgcggctg aattaccacg
     1501 aactcaacga cacgatccgc tacaccacgt ggtcggtctt ccggatcgaa ccggggaagt
     1561 tgggggagga ccgcggcgcc gcgggccgcg agacggccga gtacctcgac gggctcgagg
     1621 ccaagggcgt cgtcgtccgg ggcgtctacg acgtgtcggg cctcagggct gacgcggact
     1681 acatgatctg gtggcacgcc gaggagatcg agcaggtcca ggccgcctac accgggttcc
     1741 ggcgcacgcc gctcggccgc gcgtccacgc cggtctggag ccagaccgcg ctgcaccggc
     1801 ccgccgagtt caacaagagc cacgtgcccg cgttcctggc cggcgaggag gcgcggaagt
     1861 acatctgcgt gtacccgttc gtccggtcct acgactggta cttgctgccc gacgaggaac
     1921 gccgcaagat gctggcggac cacggcaagg aggcgcggga ctacccggac gtgcgcgcca
     1981 acaccgtcgc gtcgttcgcg ctcggcgact acgagtggat cctcgccttc gaggccgacg
     2041 aactgcaccg gatcgtcgac ctgatgcgcc acttgcgcaa caccgaggcg cgtcgccacg
     2101 tgcgcgtcga gatcccgttc tacaccggaa cgaaggtgcc gcccgccgag ctcgtcgcgg
     2161 ctctgccgta accgccggtg caggacgtac tcgccggagc ctggcagtcg ctcgccgggg
     2221 aaccgctgcc cgccgtgtcc ttgaccggcg acgaatccgt gctgcccggg cacttccggg
     2281 tccccgccat ggcaagcgcg agcatcgccg cggccacctt gtccgccggt gaattgctgc
     2341 ggctgcgcgg catcgacccg ggcacggtgt cggtcgacac ccgccacgcc gtcgccgcgt
     2401 tccgcagcga gcacctgctg cgcatcgacg gcgagcccgg accgagcccg tggggccgca
     2461 tcgcgggcga ctaccgcgcc gccgacggct ggatcaagct gcactgcaac acctcacggc
     2521 acgaggcagc ggtctgctgg gcgctcggcg tcccggcgac ccgcgaagcg gtgaccaagg
     2581 ccgtggccgg gaagaccacg gcagaggtcg aatccgccgt ggtgaccgcg ggcggcgcgg
     2641 cggcgttgat gcgctcgcgc gaggactggc tggcgcaccc gcagggccag gctgtcgcgg
     2701 aactgccgct ggtcgagctg aaccgcatcg gggacgcgcc gaagcgcacc ctgttcgact
     2761 cggaccgccc gatgggcggc gtccgcgtgc tggaactgac gcacgtgctc gccggacccg
     2821 tcgcaggccg cgtgctggcc gcacacggcg cgaacgtgct gcacgtcggc gccgcgcacc
     2881 tgccgcggat cccggtcctg accatggaca ccggccaggg caagcgttcc gcgttcgtcg
     2941 cgctcgacac cgaaggcggc cgcgcgaagc tgaagaagct catcgcccgc gcggacgtcc
     3001 tcctccagac cttccgcccc ggcgcactcg cccgaatcgg cttcggcccg gacgagctcg
     3061 ccgaactgcg ccccggcctc gtgatcgccg acgtgtccgc gtacggctgg accggcccgt
     3121 gggcccgtcg ccgcggcttc gacagcctcg tgcagatgtc gtcgggcatc gccgaggagt
     3181 acgccaccga cgctccggca ccgctgcccg cgcaagccct cgaccacgcc accggctggc
     3241 tgaccgccgc cgccatcatg acggcaatcc gccgcaccgc caccgacggc ggcacctggc
     3301 acgcccgctt gtccctggcc ggcaccggac ggtggctcga ttccttgggc cgcaaggacc
     3361 cggaaccgtc cgaagcggac tacgcggacg tactggagac ggtcgacagc gacttcggcc
     3421 ggatgacccg ggtgcggatg gccggagaac tgccgggcgc gccgccgcac tgggaccacc
     3481 cgtcgcaccg gcccggcgcg gacacggcag tgtggtcgcc ggtcgtgcct cagccggtct
     3541 cgtaggtgct gccggtctca gcgtcgtcag cttcgtcggt ggccgcgccg gttgcgtggc
     3601 tgccgctgcg cccgtccggt ggatgcggtc agctgaggag cgccgcgtcg gtctcgaggc
     3661 ggactcgccg ttgaggcgag cgcggtcggt ctcgcgggtg ccgtgtcggt ttcgcgtggg
     3721 cactgccagt acgggtgcgc cgcatcggac ctcgcgcgga tgccgccaca gtcccgtgag
     3781 cagcgccagc tcgcggctaa atcggcataa gccctcccgt cacccgcttg tccggataag
     3841 ccgccagacc gcccaacgcg accagctcgg caggtcccgc actaccaccc gcgccagcct
     3901 gctgttgggc ccgcgcgagg accgtcgcac ccgcagtcgt cggcggcagc gaactgacct
     3961 tccgctcggc gaacgtcttg gtgatccgct gactgccgac gagacagagc cacagcgtca
     4021 atacgacctc gccgaagaca agcagcacca tggcgttcag atgagactcg cccacgtcgt
     4081 agaccagccg tcccaccccg gccgccagca cgaccagggc gaccgccgtc aacgtgtacc
     4141 cgagaccggt caatggcctg cgccggctga ccagccgccg ttgcacgaga tcggtttcga
     4201 gcgtccggat caccggcttg agcaccgacg aacgcgcgac ccacgatgcg aggcgaccgc
     4261 gtccggccgc ctggtgcaca gcctgctcaa gcggcggcga cgcaggtggc gggcccgcgg
     4321 cgacgatctc gcccagcgac ccgactcgca gctggccgcg cgcgagcagc gtcgcgatcg
     4381 cagcctcgac cagccgagga cgcccgccag ccaggtatgc gacgtcgtag acatcgcgcg
     4441 gcacggcggc cggctccggc gcggacgcct gggtggccag cttgtccttg ccggtggcca
     4501 aaatgctcgc caaacccgcg gcggtggcca cgtagacgag cagcatcggg ctcaacgcgg
     4561 cccagaatcc gttcacgaca ggtttcctcc ctcccggctc ggcagagatc ggataacacc
     4621 gccgtaacca gacatgtcgt cagcgtagtc agcaccaccg acaaaaaccg gacgggctca
     4681 gcgccgaaac cacctgcgcc gaggtttagg caggtcaccg cgggcgatga gccactccgc
     4741 gaaggcgtcg gcatactcgc gcgcccggat cgaagtcgtc ttgggccgct ccgccgcgta
     4801 cgtgtcgaaa agcggccgat accgcgggcc cagcgcctca ggcaggtccg gccgcaggta
     4861 tgcgaccagt cgccgtcgct tggcgagcag gacgtccgcc tcgatgcgca accgcgaggc
     4921 atcgaagccg gacggtgcct cggccccggt caacaggctg tccagcagcg cagcttgccg
     4981 agctgccagt tcttcccggc tcacagcgtc accgcggtcc gcagtgcgac cagttcggca
     5041 gcgagttcgg aatcggtggg gtagtcgtcg tcgcgctcca agagaacgcc cggcggatcc
     5101 gtgcgccggc gcagttcggc gagcaactcc agcacctccg gcagcaccgg atgcgcgtgc
     5161 gtgtcgtgat aaaccccgtc gcgttcgaca ccgcccgcca tgtgcacgta cgcgagccgt
     5221 tcccacggaa tcccgtccag gaacgccgcc ggatcggtgc ccacgttgcg cgcgttggcg
     5281 tacaggttcg cgacgtcgac gatcagcaga cagccggtcc gttcggtcag ctcggtgagg
     5341 aattgttcct cgctcagctc gttttccggc cattccagca gcgcggcgat gttctccagc
     5401 gccaacggta cgtccacaat ggactgtgcg agccggacgt tgtccaccag cacgtccagc
     5461 gcctcgcgcg agcgcggcag cggcatcagg tgcccggagt cgagcccgcc cgcgcggacg
     5521 aagcacacgt ggtcgctggc cagcggcgcg tcgagggccc gcgccagttc ggccagatgc
     5581 tccacgcgcg cggtgtccag cggctcggcc ccgcccagcg acagcgacac cgcgtgcggc
     5641 agcaccggca tcccgcgatc cttcagcgcc agcaggcttt ccggcaggtg cggggcgtgc
     5701 aggttctccg cgaccacctc gacccagtcg acacccggca gccgcgcgat cgacaggtcg
     5761 agttccggtc gccagccgat gccgattccc agccggtcag ccaccgcatc cacctccgcc
     5821 tccgcatcca ccgccaccac cgcacgacga gccgccgccg gacgacgttc ccgcgccgct
     5881 cctcaacgcc aagcttgtcg catggtccgg gtactgtgcg aacccgccca gcgcgaccag
     5941 cgaagcagcg ccgaccagca gcgcaccgga ctgtgcccgc tgtgccgccg gatccgtccg
     6001 caccgcgacg accatcccgc ggccggcctt ggtcagacgg gccttcggcc gctttcggct
     6061 ggtcaccgcc aggaccgtaa gaatcgccgt gaccgccagc agcacgttca accccgcagt
     6121 cggccgatgc agcgcggagc cgttcgccga gcgcaccaga ccgaccacga acagggcggc
     6181 gtagaccaga acaactcccg tagcgaagcc ggcggaggcg aaggtgacca ggccgcgctc
     6241 gcgcagttcg ttcctgatgt tccgtaccgg tacggaacat gtctccttag cgcgcagcgt
     6301 cgcggctgtc tgggtccgat tgcgcacggc cgcgtggaca gcctgctcaa ccggttcggt
     6361 cggcgttctg gagacggcgg tgagctggcc ccggctgtcg acccgcagca gcccacgctc
     6421 cagcagcgcc gcgatcgccg tatcgacggt gcggtggtga ccgcccgcga ggtaagcgag
     6481 ctgatagacg tcgagcggac ggtgcacgtc cgcaggcgcc ttgcgcagca gcggccagcc
     6541 cagttgcacc accagcgtca aggccagcgc agatccgtag atcgccagga actgcgggcc
     6601 tgagatcccc cacggctcgt gcacgacgtc tccctcctcg gcccgcgggc gggccgtgcg
     6661 cctcgccgcg gctacccgcc gcagccgcct ccaccgccgc aactgctgcc tccgccgcac
     6721 gaagacccgc tgctgcacga ggaaccaccg ctgctgcagg acgacccgcc gctcgacccg
     6781 ccgcccccgg tccagctgcc gccgccgctg atcaaagccg tgctcatttc ctcgtccggg
     6841 tacagcgcga taccgccgag cgcgaccact gccgctgcac cgacgagcaa cgttccgttg
     6901 ccgcttcggt tcgtcgcagc gaactcctca ggccgttcct cggcagcttt cgcgtcttcg
     6961 cgggcacgca ccacgatcgc gtggcccgcg tcggtcgggc gcgaggtctg cgagcccttg
     7021 cggaccccgt aagcgatcag cgtcagggtc ccggccacga cgagcagccc gatcagcccg
     7081 ccgatcggcc ggtcgagctg cgcgccgttg accgcgcgga cgatgccgat cagcagcaac
     7141 gcggcgtacc cggccacgat gccggcgcga aagccgtcca gcccgtcgcc ggcgacgagc
     7201 agtccgcgtc gttcgaggtc ctggcggatc ttctccacct ccggctggac catcggcgac
     7261 ttgcgcacca tggcgacagt cgccgttccg ccctgtgcgg cgtcgtgcac cgcgcgttcc
     7321 agcctcttgc gagccggtcg cttgccgatc gcgctcagct tgcctttgct gctcacccgc
     7381 agcagctcct gttccagcag cgtcgcgatc gcggtgtcca ccgtgcggtc cgagccgccg
     7441 gccaggtacg cgagctggta gacgtccggc tgcagcggcg cctcgacggt cgcgggcgcg
     7501 ctccgtcgcc gggaccgcgc gatcgacggc cagctgatct gcaccagcag gacgacggcc
     7561 agtgccagac cgaagatcag caggaattgc gggccggaaa tgccccacgg ttcgtccacg
     7621 gcttccccct cgtatcagcg ttgccggaac cctaaccggc cggtcgccgg aggctgtcgg
     7681 cagaactgac gcacgtgatg tccggttcgt tcccggctca gctgttcccg ccgccgcagc
     7741 ccccgccgcc acacccgcct ccgcaccccg acccgccgca tccggagccg caacccgctc
     7801 cgcctccgca tccggacgcg cagcccgacc cggcggccca gtaccccgcc ggtccggcga
     7861 acccgcccgc cgctcccgcg atggccaccc ccgtccgcct ggtcgccggg agcccgcgga
     7921 gcagagcgat ccgcagttcg cgatccggat gattcgacag cccgccgacc gcgacgctcc
     7981 cggtcaggcc gctgatcagg ctccggtccg gccacgccgc ttcggctgcg gtgcggccgg
     8041 cgtcggtcgg ccggatccgc cgggccgccc ggcgacggga cagcgcggcg aacgcgaacc
     8101 acgcgggcag cagaaccgaa atcagcacat agccgttcag cccgccgacg aacgccgcca
     8161 cgccggctgc cgccagcacc gcttcctcga tcgcggtcca cttccatgct ctccgcagcg
     8221 cctggccgtc aaccagcaaa ccgcgtttgg tgaggtcgtc gatcagctcg cggaccgtgt
     8281 cgccctcgac gagcccgcgc agcaccgtcg cgaccccgac tcccgtgccg gtcagctcgg
     8341 cagcttccct gctcagcgcg tccgacggtg cggtcggcgt gcggtacagc cgccccacgg
     8401 agtccgcgcg caactgttcc cgttccagca tcgaggcgac cgtcgtttcg gcgacgcgga
     8461 tcggtccgtt ggtcagcaac gcgacctgcg ccgcggacgt cagccgggtc tcaggatgcc
     8521 tgccccggtt cagccgggcg gcggtccgcc gtccgagcca ccagcaggcc agcgccagca
     8581 ccaggatcag cgcggccggc atcgcgcgtt cgatcgtcca gtccatcacg gtccccctcc
     8641 ggatgaacaa accacgttac ccgcctgcgc ggggttgccg ccggacgaaa aaccgccctc
     8701 cccggagacg ggaagggcgg tttcagcgga cctcggcgtc agtcgccggc gtcctcgacg
     8761 agcttcaggg aaatcgagtt gatgcagtac cgctggtcgg tcggggtcgc gtagccctcg
     8821 ccctcgaaca cgtgcccgag gtggctgtgg cacgaggcgc acagcacctc gatgcgccgc
     8881 atgcccatgg tgcggtcctc gcgcagcagc accgcgtcgg agtccgccgg gtcgtagaac
     8941 gacggccagc cgcagtggct ttcgaacttc gtgtcgctgc ggaacagctc cgcgccgcaa
     9001 gcccggcact cgtacacgcc ggtggtcttg gtgtcggtgt actcgccggt gaacggccgt
     9061 tcggtgccgg cttgccggag caccgcgtac tcctcggggg caagctgctc ccgccattcc
     9121 tggtcggact tcaccacgcg cggagtggtg ccgacgacgg gtttcatacc tttcaccctt
     9181 cccacgttac ctggagagaa aagcgaccgt gtgcagcgcg atgtcccagg cggtgaccag
     9241 cagcccgagc acgatcagca ccacgaccag cgcggacacc cagtagcgga gcccgccggt
     9301 gcgggtgctg gaatcggcga agtcggcgac ctggtccagg cacgcctcga cggtgaaagt
     9361 cggcccggtg cgctccatcc ggtcgagatg ggcggcgaac gcacgggcct ccgggtcgta
     9421 cggatccagc ccgaccagat cctcgtggaa ccgaggattc cagcccggcg gcagagctcc
     9481 gttctcggcc atgcctcaac ggtaggctat tcggcctgcc ggacccacct ctgcggcgag
     9541 tgtcactgcg cgaaggaact cgctaggtgc ccgaggccgg agcatcaacc tttgtgttgc
     9601 tgtcgatgcg catctgcccg ccaaggatca cgatgtggta gggacggcga acgccgttga
     9661 gttccatcgt cagatccagc gagccgtcgg ggttggggga gcccagagcg cgagcgctcc
     9721 ggtacccctg aaccttgtgg ctgtcgtccg cccaataggc cgcgtaagcc tcttgagagc
     9781 cgtacactgc ttgcgccgcg ggcgtcagca agttccagcc ttggccgggg tccggataat
     9841 agctgtcggt gagaaagtgg ccggccgcac cgtagtcggt gatcttgcct tccgacgggg
     9901 ttttgccgag acccgcaggg ctggcggagc tactgggcgc actgctgggc ggagcggtct
     9961 ggctcgcagc cgactggccg cccggcgtct gcgcgttcga cgaaggcccg cccttgtccc
    10021 ggctgctgct caaaaccaaa aacaccacca acgccaccgc gacaaccgcc acggccccgc
    10081 cagcgaggaa cgccatcttc ttcttccgcg ccgcagcagg cgaaacccga tcccccgacc
    10141 cagcaccgga ataattcggc gctgcggcag taggcgtcgg ccgcaccgcg ggctgcggac
    10201 gcggcggcgt gttcgccggg ggagcaggac gcgaaggcgc gcccatcggc atgaatgccg
    10261 cagtcggcgt ccgaggcggg ttcgacggag ccttcggcgc cgcagcagca gccgccggag
    10321 cagccgtacg cgcccacggc ggacgagacc cgttgctccc cggaccgcga ttccccgaaa
    10381 gcaactgctg cggcgcagcc ggttccccag tcgccaacgc ggccagccgc tcccgcgcct
    10441 cggccatgct cggccgctcg gaagcttcgc tgcgcagcag gctcatcagc aacgcggtcg
    10501 ccggaccggc ctgcgtcggc gggttgacct ggccgttcgc cgccgcgtag agcagggcga
    10561 gctggttcgt ggtgttgccg tacggggtct gtccctcgat cgcctggtat agcgtcgcgc
    10621 cgagggcgaa gacgtccgag ctgggcaccg ggtcggcacc gcgggccagt tcgggggcga
    10681 ggtacgccgg ggtgccgccg atgaggccgg tctgggtgag cgtcatgtcg ccggccgcgc
    10741 gggagatgcc gaagtcggtg atcttcgcgg tgccggtttc gtcgatgagg atgttgccgg
    10801 gctt
//
