LOCUS       NZ_AP025344            10384 bp    DNA     circular CON 24-MAR-2024
DEFINITION  Paenibacillus dendritiformis strain J27TS7 chromosome, complete
            genome.
ACCESSION   NZ_AP025344
VERSION     NZ_AP025344.1
KEYWORDS    RefSeq.
SOURCE      Paenibacillus dendritiformis
  ORGANISM  Paenibacillus dendritiformis
            Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae;
            Paenibacillus.
REFERENCE   1
  AUTHORS   Okumura,K., Okamoto,M. and Takamatsu,D.
  TITLE     Whole-Genome Sequences of Bacillus and Paenibacillus sp. Strains
            Isolated from Honey in Japan
  JOURNAL   Microbiol Resour Announc 11 (11), e0084222 (2022)
   PUBMED   36301092
  REMARK    DOI:10.1128/mra.00842-22
REFERENCE   2  (bases 1 to 6547390)
  AUTHORS   Takamatsu,D., Mada,T., Okumura,K. and Okamoto,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2021) Contact:Daisuke Takamatsu National Institute
            of Animal Health,NARO; Kannondai3-1-5, Tsukuba, Ibaraki 305-0856,
            Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 3
            Genome Coverage                   :: 124x
            Sequencing Technology             :: PacBio; NovaSeq 6000
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_021654795.1-RS_2024_03_24
            Annotation Date                   :: 03/24/2024 09:12:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,875
            CDSs (total)                      :: 5,765
            Genes (coding)                    :: 5,679
            CDSs (with protein)               :: 5,679
            Genes (RNA)                       :: 110
            rRNAs                             :: 8, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 39 of 86
            Pseudo Genes (incomplete)         :: 62 of 86
            Pseudo Genes (internal stop)      :: 15 of 86
            Pseudo Genes (multiple problems)  :: 26 of 86
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 05:45:11
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 5419928
            Orig. end                         :: 5430312
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP025344.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10384
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="16"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10384
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10384
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5384](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5384)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    complement(244..260)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 21.0"
                     /tool="antismash"
     gene            1080..3023
                     /db_xref="GeneID:73387350"
                     /locus_tag="L6439_RS24070"
                     /old_locus_tag="PsJ27TS7_49110"
     CDS             1080..3023
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:73387350"
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /locus_tag="L6439_RS24070"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PsJ27TS7_49110"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_213471165.1"
                     /transl_table=11
                     /translation="MSKGYRRRLPPSYATHNFVLAHLPNYVYTHFISYLFFTMMANIDE
                     RREMMHSFSNIWNSSRFAILYIWRLHRPYFYVSVALYLALGFLPLLSVWTMQELVNEVV
                     FIAQPGHGFARALSMLSFQMVIILSAYILQFFSQLNDQKLENQLGLVLKKQIFGKIMNL
                     PYPAFEDPSFYDQNQRIINSHFQIVSLVKTILSLGSTIITVLSLVGYITNIHWGLLVII
                     FVFSIPILMIHIRFGNKRYALTRFLTPYQRREMYISDLLSQRDSLKEIRLFGLGPHLID
                     KWSSFYKRSAQEKYNLSKNQGIWELAGSMLLTLTYIVSGILIILLISAKRLEIGAFVAA
                     LQSIQNIQSSLTEISASLSNIYEISLYIEDFNSFQQKKELSSSPSRIKCGAIHRISVQN
                     LTFTYPNQTKPALKNIHLSIEKGQKIVIIGENGSGKTSLIKCILGLYETGEHTICVNQI
                     PLRHYDISSYHQRISALFQDFIEFEFSARENIGFGNLNNPSKPEVIAAAKKTKIHEHIS
                     NLPEQYESLLGRHFEGSSELSGGQWQKLALSRALFKNGDMIILDEPTSSMDPESELDVI
                     HELFQGNNDKAVLIITHRLGVAVQADYILVMKEGQIVEEGTHKELLRRQGEYHRIYRAQ
                     TKIYNQESGVFV"
     gene            3026..3457
                     /db_xref="GeneID:73387351"
                     /locus_tag="L6439_RS24075"
                     /old_locus_tag="PsJ27TS7_49120"
     CDS             3026..3457
                     /codon_start=1
                     /db_xref="GeneID:73387351"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="L6439_RS24075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="PsJ27TS7_49120"
                     /product="hypothetical protein"
                     /protein_id="WP_213471164.1"
                     /transl_table=11
                     /translation="MALVLMFRHVLAFLFFSTFLHKARNYREHIRIMNNTTCQTCKSIL
                     PDLKRAVDTWDMHIVVINTDKQSDDEEIQAMLPSGIDYIRSLDIMEAYGVSKVPYAFVV
                     NQNGTLEFHQSLLNSNMLWNMLANESARSHDRVPNSLSI"
     gene            complement(4009..4911)
                     /db_xref="GeneID:73387352"
                     /gene="thpD"
                     /locus_tag="L6439_RS24080"
                     /old_locus_tag="PsJ27TS7_49140"
     CDS             complement(4009..4911)
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /db_xref="GeneID:73387352"
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006679980.1"
                     /locus_tag="L6439_RS24080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PsJ27TS7_49140"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_168178133.1"
                     /sec_met_domain="PhyH (E-value: 6.9e-37, bitscore: 126.1,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTGQSQVQQAARTPDLYPSRVNTETLLLKRQDPVVYNHAIGQAEI
                     TADQLASYEADGYLFLDSFFSEDEVQAWKRELVRLFQDYKGSAKPEVIREPGSEEIRSI
                     FAVHREGIFQQLANHPRIIKIVEQILGSQVYVHQSRINFKQGFTGKEFYWHSDFETWHV
                     EDGMPRMRALSCSIALEENNANNGPLMVIPGSHRTFVSCIGATPDDHYKSSLRRQEYGI
                     PDRDSLIHLANQGGIAAPTGKAGSILLFDCNVMHGSNSNITPFPRSNVFIVYNSVDNQL
                     EPPYSGKPPRPDFIAARSF"
     gene            complement(5001..5384)
                     /db_xref="GeneID:73387353"
                     /locus_tag="L6439_RS24085"
                     /old_locus_tag="PsJ27TS7_49150"
     CDS             complement(5001..5384)
                     /codon_start=1
                     /db_xref="GeneID:73387353"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006679979.1"
                     /locus_tag="L6439_RS24085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PsJ27TS7_49150"
                     /product="ectoine synthase"
                     /protein_id="WP_168178134.1"
                     /sec_met_domain="ectoine_synt (E-value: 1.3e-51, bitscore:
                     172.2, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVKQLSDILGTEQDVKAETWNSRRLLLKKDGVGFSLHDTLIKAG
                     TETEIWYKHHVEAVYCIEGEGEIEVVESGAVYPIQPGTLYVLDGHEKHLLRGKSQMRML
                     CVFNPPCTGNEVHDADGAYPLEV"
     gene            complement(5447..6730)
                     /db_xref="GeneID:73387354"
                     /gene="ectB"
                     /locus_tag="L6439_RS24090"
                     /old_locus_tag="PsJ27TS7_49160"
     CDS             complement(5447..6730)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (31-366). E-value: 7.2e-79.
                     Score: 257.2. Matches aSDomain:
                     nrpspksdomains_L6439_RS24090_Aminotran_3.1"
                     /codon_start=1
                     /db_xref="GeneID:73387354"
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006679978.1"
                     /locus_tag="L6439_RS24090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PsJ27TS7_49160"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_213471163.1"
                     /sec_met_domain="Aminotran_3 (E-value: 5.7e-77, bitscore:
                     257.5, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKVLDGRTPSLQVFDSLESEVRSYCRSFPAVFTKAQGYKLWDADH
                     REYIDFFAGAGALNYGHNNRAMKRKLLDYIQQDGVTHSLDMATKAKERFLQKFHQVILK
                     PRGLEYKVMFPGPTGANSVESALKLARKATGRATVFSFTNAFHGMTLGALSVTGNKFKR
                     RGAGIPLHHTVTMPYDGYLGPDIDTLDYMECYMNDSGSGVSRPAAIIVETVQGEGGINE
                     ASNEWLQRLERICRSHEIVLIVDDVQMGCGRTGTFFSFERAGITPDIVCLSKSIGGYGL
                     PMALTLIRPDLDIWEPGEHNGTFRGNNLAFITAAEALDYWKNERFTQDIVAKGKIIRSC
                     LEELVAQYPELQGTARGRGLMQGIAFGIPGMAERLCARAFAKGLIMETSGTDSEVAKIM
                     PPLTIDDTGLLAGLDILRTCVKEMAEEL"
     aSDomain        complement(5633..6637)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_L6439_RS24090_Aminotran_3.1"
                     /evalue="7.20E-79"
                     /label="L6439_RS24090_Aminotran_3.1"
                     /locus_tag="L6439_RS24090"
                     /protein_end="366"
                     /protein_start="31"
                     /score="257.2"
                     /tool="antismash"
                     /translation="FTKAQGYKLWDADHREYIDFFAGAGALNYGHNNRAMKRKLLDYIQ
                     QDGVTHSLDMATKAKERFLQKFHQVILKPRGLEYKVMFPGPTGANSVESALKLARKATG
                     RATVFSFTNAFHGMTLGALSVTGNKFKRRGAGIPLHHTVTMPYDGYLGPDIDTLDYMEC
                     YMNDSGSGVSRPAAIIVETVQGEGGINEASNEWLQRLERICRSHEIVLIVDDVQMGCGR
                     TGTFFSFERAGITPDIVCLSKSIGGYGLPMALTLIRPDLDIWEPGEHNGTFRGNNLAFI
                     TAAEALDYWKNERFTQDIVAKGKIIRSCLEELVAQYPELQGTARGRGLMQGIAF"
     gene            complement(6727..7266)
                     /db_xref="GeneID:73387355"
                     /gene="ectA"
                     /locus_tag="L6439_RS24095"
                     /old_locus_tag="PsJ27TS7_49170"
     CDS             complement(6727..7266)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:73387355"
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006679977.1"
                     /locus_tag="L6439_RS24095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PsJ27TS7_49170"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_168178136.1"
                     /transl_table=11
                     /translation="MKANVTRSLPCAIRNARKGDGTELWKMATESGTLDVNSAYCYIML
                     CEYFADTCFVAEQDRKLIGFVSALRPPEMPDTLFVWQIAVRDGYRGRGIAEALLRTLHD
                     AACRARVRFIETTITPSNTASQRLFAKMAKTWKSALVTKAGFSAHLFPEGAHETEKLIR
                     IGPLRHNTHDRMETVT"
     gene            complement(7269..8135)
                     /db_xref="GeneID:73387356"
                     /gene="ehuA"
                     /locus_tag="L6439_RS24100"
                     /old_locus_tag="PsJ27TS7_49180"
     CDS             complement(7269..8135)
                     /GO_component="GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_process="GO:0033308 - hydroxyectoine transport
                     [Evidence IEA]"
                     /GO_process="GO:0051470 - ectoine transport [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:73387356"
                     /gene="ehuA"
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006679976.1"
                     /locus_tag="L6439_RS24100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PsJ27TS7_49180"
                     /product="ectoine/hydroxyectoine ABC transporter
                     ATP-binding protein EhuA"
                     /protein_id="WP_168178137.1"
                     /transl_table=11
                     /translation="MESVPCRPLALDTAHEPIVIYRDICKSYGSVSVLQDINLSINPGE
                     KVAVIGPSGSGKTTLARLLMTLERPTSGTIQVDGEWLWHQEVKGKLVAAKEKHLHNVRG
                     KIGMVFQHFHLFPHMTILRNVTEAPVHVLGWSKEEAEARALEMLAKVGLADKVDEYPAR
                     LSGGQKQRVAIARAVVMRPKVMLFDEATSALDPELVGEVLAVIKDLAAEGDMAMMLITH
                     EMDFAREIADRIIFTDGGAIVEQGTPEQIFEHPESPRLQAFLSRFRQTWYKGGFQQASS
                     SAEEEGI"
     gene            complement(8152..8802)
                     /db_xref="GeneID:73387357"
                     /gene="ehuD"
                     /locus_tag="L6439_RS24105"
                     /old_locus_tag="PsJ27TS7_49190"
     CDS             complement(8152..8802)
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_function="GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_process="GO:0033308 - hydroxyectoine transport
                     [Evidence IEA]"
                     /GO_process="GO:0051470 - ectoine transport [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:73387357"
                     /gene="ehuD"
                     /gene_functions="transport (smcogs) SMCOG1096: polar amino
                     acid ABC transporter, inner membrane"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006679975.1"
                     /locus_tag="L6439_RS24105"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PsJ27TS7_49190"
                     /product="ectoine/hydroxyectoine ABC transporter permease
                     subunit EhuD"
                     /protein_id="WP_168178138.1"
                     /transl_table=11
                     /translation="MWSWDYALEILPQLLRVLPITIGATLLGFAAACLLGLPLALARRS
                     RYRAVSVLANAWMEFIRSTPLLVQLFVLFYALPLYGISFSPFVTGVIGLGLHYSAYLSE
                     VFRSGIEAVPAGQWEAAKALNFGRRKSWTNIILPQAIPPIIPVMGNYLITMFKETPVLS
                     AITLVELLQTAKAIGSGSFRYLEAFTLVGLLFLLLSYPSSLLVGRLERKYQIK"
     gene            complement(8806..9465)
                     /db_xref="GeneID:73387358"
                     /gene="ehuC"
                     /locus_tag="L6439_RS24110"
                     /old_locus_tag="PsJ27TS7_49200"
     CDS             complement(8806..9465)
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_function="GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_process="GO:0033308 - hydroxyectoine transport
                     [Evidence IEA]"
                     /GO_process="GO:0051470 - ectoine transport [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:73387358"
                     /gene="ehuC"
                     /gene_functions="transport (smcogs) SMCOG1096: polar amino
                     acid ABC transporter, inner membrane"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006679974.1"
                     /locus_tag="L6439_RS24110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PsJ27TS7_49200"
                     /product="ectoine/hydroxyectoine ABC transporter permease
                     subunit EhuC"
                     /protein_id="WP_213471162.1"
                     /transl_table=11
                     /translation="MRTSAMLEYAPALLQGAWVTLQIVCFSMMLAFFCAFAAGLARLSK
                     YRIVRFVSGVYVEVFRGTSLLVQLFWLYFALPMLLDVRMPAMTAAVLALGLNYGAYGSE
                     VVRGSILSVPKGQYEAAIALNMSPWLRMRNIILPQAMALMLPSFGNLLIELLKGTSLVY
                     LITLMDLTYQGMVLRSFDNSSTPQIFALLLLIYFIMAYTLTLGIRFLERRAVKGRI"
     gene            complement(9470..10372)
                     /db_xref="GeneID:73387359"
                     /gene="ehuB"
                     /locus_tag="L6439_RS24115"
                     /old_locus_tag="PsJ27TS7_49210"
     CDS             complement(9470..10372)
                     /GO_component="GO:0009897 - external side of plasma
                     membrane [Evidence IEA]"
                     /GO_component="GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /GO_function="GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033294 - ectoine binding [Evidence IEA]"
                     /GO_function="GO:0033295 - hydroxyectoine binding [Evidence
                     IEA]"
                     /GO_process="GO:0033308 - hydroxyectoine transport
                     [Evidence IEA]"
                     /GO_process="GO:0051470 - ectoine transport [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:73387359"
                     /gene="ehuB"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1091: glutamine-binding lipoprotein glnH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006679973.1"
                     /locus_tag="L6439_RS24115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PsJ27TS7_49210"
                     /product="ectoine/hydroxyectoine ABC transporter
                     substrate-binding protein EhuB"
                     /protein_id="WP_168178140.1"
                     /transl_table=11
                     /translation="MKKWYSYALCCVMAILLASCASQPGDSRTEPNGGTSSGQETALEK
                     ARREGYVTVGFANEKPYAYATPDGQLTGEAVEVAREVLTRMGIEEMNGVLTEFGSLIPG
                     LKAKRFDIITAGMFINPERSKEVAFANPEYSIGEALAVKKGNPSNLRSYEDIARSKDVK
                     VAVMVGAIELEYLAKSGVAKEQIVTVPDQPSAISALQAGRVDAVTMTGPSLQAMLDSTK
                     DDQLERVMDFEQPEIDGKSVRGYGAAAFRKEDADFREAFNQELEALKQSGKLLELLEPF
                     GFTEQELPGDMTAERLSQE"
ORIGIN
        1 atggatgcgc ttcgccgcga cctggaccgg ctgcgggacc ggtactacga tgtgccgttc
       61 tccgaggtga gcctcggcga gtctctccat gacttgttcg ccgtcgccga gaagcaccgg
      121 atcgtgttcc ctcccgatct gattctgttg gcgaaggcgc tgctcacctt ggaaggcgtc
      181 gtggagaggc ttgatccgac cctctccatc ctcgatatgg ccgagccgtt cggcgtgaag
      241 ctgctgaagg aaaaatacag cttccgccgc attcagaaga aggtggtcgg cggcgcgctc
      301 gatctggcgc aagcggtgtc cgatctgccg atgcaggcga gccggctgtc ctccctcctc
      361 agccggggca agatcaaggc cgaaatggag ctgcgcgatc tggagcatgt gctgcacaag
      421 ctcgatcaga tcagcaatcg gctctccttc agcattgtgc tgcttgcctt cagcatcatt
      481 atggtcgggc tgatcgtcgg ctcctcgctc agcaggaagc cgacgctgct ctgggatatt
      541 cccgcgatcg agatcggctt ccttatcgcg gccttgatgt tcatctggct gcttctgtcg
      601 attttccgct ccggcagatt ctagcaagac agggcgggga actggccgat gacgcttctc
      661 cccgcctcta tcccctagac tccgcgcaac tgtgaagcag ttccatcttc ccccgccatc
      721 cctccggatc cggagaagca taatcaatct cttgcttgga attcacatag acaaagtaag
      781 gatattccgt aacccggcag gcttcgaata atttctgtcc ggcttgcaaa aatcggatcc
      841 ctgtactatc gctatagagg tctttgtagc cgccaattcc gccctcctgc agcaaaacgg
      901 ccgcatgggg atcggatccc cgctccctat ttgactgaat ccatctctct agtgaaggga
      961 tgcagtaagg gcaatgagaa tcgataaaaa taagcagtat tcccttcttc tctatgacat
     1021 gggttaaatc aaagagagta ccgtcggcat ccggcaagac aatagaggga gcttgctggt
     1081 tgagcaaagg gtacaggcgg cggctccccc cttcctacgc cactcataat ttcgtccttg
     1141 cgcatctacc aaattatgta tatactcatt tcatatcata cttatttttt actatgatgg
     1201 caaatattga tgaaaggcga gaaatgatgc attctttttc taatatttgg aacagttccc
     1261 ggtttgctat tctttatatc tggagattgc acagacccta tttttacgtc tccgttgcgc
     1321 tttatctcgc gctaggtttc ttgcctttgc tgagcgtgtg gacgatgcaa gaactcgtga
     1381 acgaggttgt tttcatcgct caacccgggc atggattcgc gagagcgttg tcgatgttaa
     1441 gcttccaaat ggtcatcatc ctttctgcct atattctgca atttttctca caattaaatg
     1501 atcaaaaact tgaaaatcag ctcggactgg tgcttaaaaa acagatcttc ggcaaaatta
     1561 tgaatctgcc ctacccggcc tttgaggatc cgtcttttta tgatcagaac cagcgaatta
     1621 tcaatagcca cttccagatc gtttccctgg tcaagaccat cctgtctttg ggaagcacca
     1681 tcatcacggt tttgtcttta gtcggctaca ttaccaatat tcattgggga ttgctggtca
     1741 ttatctttgt attttcgatt ccgatcctga tgattcatat ccgcttcggc aataaaagat
     1801 atgcgctcac tcgatttttg actccctatc agagaagaga aatgtatata tccgatctcc
     1861 tgagccaacg agactctttg aaggaaatcc gcctctttgg actgggcccg cacctgatcg
     1921 acaaatggtc ttcgttctat aagcggagcg cgcaagaaaa atacaatttg tccaaaaatc
     1981 aaggcatatg ggagcttgcc ggcagtatgc tgctcacatt aacctatatc gtctccggga
     2041 ttctcattat cctgctgatc tcggccaaga ggttagaaat cggggcgttt gtggcggcct
     2101 tgcagtcgat tcaaaatatt cagtcgagtt tgaccgagat ttccgcatcg ctgtccaata
     2161 tttatgagat cagtttatat attgaagatt tcaactcttt tcaacaaaaa aaggagttgt
     2221 caagttcacc ttctcgcatc aaatgcggcg ctatccatcg aatcagcgtg caaaatctca
     2281 cgtttactta tccgaatcaa accaaaccgg ctctcaaaaa tattcatctg tctattgaaa
     2341 aaggacaaaa gatcgtcatc atcggagaaa acggctccgg caagacctcg ctgatcaaat
     2401 gcatactggg actctatgaa acgggtgaac atacgatatg cgtcaaccaa attcctttgc
     2461 gccattatga tatttcctcc taccatcaac ggatttccgc tttatttcag gatttcatcg
     2521 aatttgaatt cagcgcccgg gaaaatatcg gattcggcaa tctgaacaac ccgagcaagc
     2581 ctgaagtaat cgctgccgca aaaaaaacga aaattcatga acatatctca aatctgcctg
     2641 aacaatatga atccctgctc ggacgtcatt ttgaaggaag cagcgaatta tccggcggtc
     2701 aatggcaaaa gctggccttg tccagagctc tcttcaagaa cggggatatg attattctgg
     2761 atgaacccac ttcatccatg gacccggaat cggagcttga tgttatccat gaattatttc
     2821 aagggaacaa tgacaaagcg gtgctgatta ttacgcatcg tcttggcgtc gccgtgcagg
     2881 cggactatat tttggtgatg aaggaaggtc aaatcgtcga ggaaggcaca cataaggagc
     2941 ttttgcgccg acaaggagaa taccatcgca tctatcgcgc tcagaccaag atttataacc
     3001 aagaaagcgg ggttttcgtc taaacatggc tctcgttttg atgttccggc atgtacttgc
     3061 ctttttgttt ttctccacct ttttgcataa agcgcggaac tatcgtgaac atattcgcat
     3121 catgaacaat acgacatgcc agacctgcaa aagcatactg cctgacttaa aaagagcggt
     3181 tgacacttgg gatatgcaca tcgtcgttat caatacggat aagcaatccg atgacgaaga
     3241 gattcaagcg atgctgcctt ccgggatcga ttatattcgc tccctggaca tcatggaagc
     3301 ctatggcgtc tcaaaagttc cctatgcttt tgtcgtgaat caaaacggaa cacttgagtt
     3361 ccatcaatcg ttactgaatt cgaacatgct gtggaatatg ctggcgaatg aatcggcacg
     3421 atcccatgac cgggtcccca attccttatc gatatgaatg agcagcgttc gcatgggaag
     3481 aaaaacggac aagaagagaa gcgattttgc gccttgtcag aggcgaaatc gcttccctgg
     3541 ttcaatgcat tctcatgctg gacaacatag gcttgtgcct agcaaccgca tcttacttta
     3601 cttggcctcg tacactcttc ccccgtatcc cgatcgacat agtactccca ggaggatact
     3661 ttttggataa tgcaaagggc gtcggagcgg caggagacga ctctgcgcgt taagatgcaa
     3721 cgtgcggcgg gaggctggaa tccggtcaac ttgcttaaaa agtttttcac tttcattacc
     3781 tccattacca attactttta tttcatgtat ataatatatc gtttctacca taaaatccat
     3841 atttcgtttt actatcaaat gtgaaatgcc ggctatcgca aagcaatccg tctcctattt
     3901 atggataatt ttatagccta tgccgggcgg acggcaaaag aagacccatc cgcgatgggt
     3961 cttctccata ttgagcgagc aagcgattca ccgctctctc atcctcagct agaatgaacg
     4021 ggccgcgata aaatcaggtc ttggcggctt gcctgagtac ggcggttcaa gctgattatc
     4081 gacactgtta tagacaatga atacattgct gcgcgggaac ggcgtaatat tgctgttcga
     4141 gccatgcatc acgttgcagt cgaacaatag aatcgagccg gccttgcccg taggcgctgc
     4201 aatgccgcct tgattcgcca gatgaataag actgtcccga tcgggaatgc cgtattcctg
     4261 acggcgcagc gaagatttat aatggtcgtc cggcgtggcc ccgatacagg agacgaacgt
     4321 gcggtgcgag ccggggatga ccattaatgg accgttgttc gcattgttct cttccaaggc
     4381 aatggagcag ctcaatgccc tcatgcgggg catgccgtct tccacatgcc atgtctcgaa
     4441 gtcggaatgc caatagaact ctttgcccgt aaagccttgc ttgaaattaa tgcgcgattg
     4501 gtgcacatag acctgactgc cgagaatctg ttccacgatc ttgataattc tcgggtgatt
     4561 cgccagctgt tggaagatgc cttcccggtg cacggcaaaa atcgagcgta tctcttcgct
     4621 gcccggctcc cgaatcactt ccggcttcgc gctgccctta taatcctgga acaagcggac
     4681 cagttcccgc ttccatgcct gcacctcgtc ctcgctgaag aacgaatcca ggaacaagta
     4741 tccgtccgct tcataagaag caagctgatc ggccgttatc tccgcctgcc cgatcgcatg
     4801 gttgtagaca accggatcct ggcgcttcaa taataaggtc tccgtattca ctctggacgg
     4861 atacagatcc ggcgttcttg cggcctgctg aacttggctt tgtcctgtca atatgatcgt
     4921 gctccttccg taaaagagaa tggcggttcg cgttcccgct tttttcgtca tcgcttctat
     4981 tcgttcgctt gcaaccacgc ttagacttct aacggatatg cgccgtccgc atcatgcacc
     5041 tcattgccgg tgcagggcgg attgaagacg cacagcatgc gcatctggct cttccctctc
     5101 agcagatgct tctcgtggcc atcgagcaca tataacgtgc cgggttgaat cggatagacg
     5161 gctccgctct cgacgacctc gatttcccct tctccctcga tgcagtagac cgcctccaca
     5221 tgatgtttat accagatttc ggtctccgtg cctgccttaa tcagcgtatc atgcaaggaa
     5281 aaaccgaccc cgtccttctt gagcagcaac ctgcggctgt tccatgtctc cgccttcaca
     5341 tcctgttccg taccgagaat atcggacaat tgcttgacga tcatcgcaaa ttcctcctaa
     5401 ttgttcatgt tctccaacat tgcacgtgcc gcgagagaat ggctggctac agctcttccg
     5461 ccatttcctt cacgcatgtt cgcaaaatat ccaagcctgc aagcagaccc gtgtcatcaa
     5521 tcgtgagcgg cggcataatc ttcgccactt cgctgtctgt acccgatgtc tccatgatga
     5581 gccctttcgc aaaggctctg gcgcagagcc gctccgccat gcccgggatg ccgaaggcga
     5641 tcccctgcat caatccccgg cctctcgccg ttccttgtag ttcaggatat tgtgcgacca
     5701 gctcttccaa gcaagagcga atgatctttc cttttgccac gatatcttgc gtaaagcgtt
     5761 cattcttcca atagtcaagc gcctcggccg cagtaataaa agccaagtta ttccccctga
     5821 acgtgccgtt atgctcgcca ggctcccaga tatcgaggtc ggggcggatt aacgttagcg
     5881 ccatgggcag cccatacccg cctatcgatt tggagaggca gacgatgtcc ggcgtaatgc
     5941 ccgcccgctc gaagctgaag aaggtccctg tccggccgca ccccatctgc acatcgtcca
     6001 cgatgagcac aatctcgtgg ctgcggcaaa tccgctccag ccgctgcagc cattcattgc
     6061 tcgcctcgtt aatcccgcct tccccttgaa cggtctccac gatgatggcg gccggccgcg
     6121 acaccccgct tccgctgtca ttcatatagc attccatata gtcgagcgtg tcgatatccg
     6181 gacccagata tccgtcataa ggcatcgtga ccgtatgatg cagagggatg cccgcccctc
     6241 gccgcttgaa cttgttgccc gtgacggaca aggcgccaag cgtcatgccg tggaaggcat
     6301 tcgtgaagct gaagacggtc gctcgccccg ttgccttgcg cgccagcttc aaggcgctct
     6361 ctaccgagtt cgcccctgtc ggtccgggga acatcacctt gtattcaagt ccccgcggct
     6421 tcaaaatgac ttggtgaaat ttctgcagaa accgttcctt cgccttcgtg gccatatcca
     6481 ggctatgcgt cacgccgtct tgctgaatat agtccagcag cttccgcttc atcgcccggt
     6541 tgttatgccc gtagttgagc gctccggctc cggcgaagaa atcaatatat tcgcgatggt
     6601 cggcatccca cagcttatag ccttgcgcct tcgtaaatac ggccgggaac gacctgcagt
     6661 agcttcgcac ctcagactcc aacgaatcga atacttgcag cgatggcgtt cttccatcca
     6721 ataccttcat gtcaccgtct ccattctatc gtgtgtattg tggcgcaaag gccctatgcg
     6781 gatcagcttc tccgtctcat gagcgccttc cggaaagaga tgagcggaga atcccgcctt
     6841 cgtcacgagc gcgcttttcc atgttttcgc catcttggcg aacaggcgct gggaagccgt
     6901 gttgctcggc gtgatcgtcg tctctataaa ccgcacccga gcccggcatg ccgcatcatg
     6961 caatgtgcgg agcagcgctt cggcgatccc tctgcctcga tagccgtcgc gcaccgcgat
     7021 ttgccatacg aaaagcgtgt ccggcatctc tggcggccgc agcgcggata caaacccgat
     7081 aagtttccga tcttgttcag ccacgaagca ggtgtcggcg aaatattcgc acagcatgat
     7141 atagcaatag gccgagttga catcgagcgt cccgctctcg gtcgccatct tccataattc
     7201 cgttccatcg cccttccgcg cgttgcggat tgcgcagggc aaggagcgcg ttacatttgc
     7261 tttcatgctc agatcccctc ctcttccgca gatgatgaag cttgctgaaa tcctccctta
     7321 taccacgtct gtctgaagcg gctcaaaaaa gcttgcaaac gcgggctctc cgggtgctcg
     7381 aagatttgct ccggggttcc ctgttcgacg atggcccccc catccgtaaa gatgatccga
     7441 tcggcaatct ccctggcaaa atccatttca tgcgtgatga gcatcatcgc catgtcgcct
     7501 tcggccgcca agtccttgat gaccgccagc acctcgccga ccagctccgg atcgagcgcg
     7561 gaggtcgctt cgtcgaacag catcaccttc ggacgcatga ctacggctct ggcaatcgcg
     7621 acgcgctgct tctgcccgcc ggacaagcgc gcagggtatt catccacctt gtctgccagc
     7681 ccgaccttgg ccagcatttc cagcgcgcgc gcttcggctt cctccttgct ccatcccaat
     7741 acgtgcaccg gcgcttccgt tacattgcgc aaaatggtca tatgcggaaa aaggtgaaag
     7801 tgctgaaaga ccatgccgat ttttccgcgt acgttgtgca gatgcttttc cttcgccgct
     7861 acaagcttcc ccttcacttc ctggtgccac agccactccc catcgacttg aatcgttccc
     7921 gaggtgggcc gctccaatgt catcaggagg cgggccagcg tcgtcttgcc cgatccgcta
     7981 gggccgatga cggcaacctt ttcccccgga ttaatggata gattaatgtc ctgcagcacg
     8041 ctcactgagc cgtatgattt gcagatgtcc cgataaatga cgattggttc gtgcgccgtg
     8101 tccagggcca gcggtctgca tggaacgctt tccatctttg ctctacaccc cctatttgat
     8161 ctgatacttc ctctccaatc tgccgacaag cagagatgac ggatagctaa gcagcagaaa
     8221 caataagccg accagcgtaa aggcttccag ataacggaat gagccggaac ctatcgcttt
     8281 cgccgtttgc agcagttcga ccagcgtaat cgccgataag acgggagttt ctttgaacat
     8341 cgtaatcaaa tagttgccca tgacgggaat gattggcggt atggcctggg gaaggatgat
     8401 gttcgtccat gacttccgcc tgccgaagtt aagcgccttg gccgcttccc attgaccggc
     8461 gggcaccgcc tcgattcccg agcgaaatac ttcagaaaga taagcgctat aatgcaagcc
     8521 caatccgatg actccggtga caaacgggga gaatgaaatg ccgtaaagcg gtaatgcgta
     8581 aaagagcacg aacaactgaa cgagcagcgg agtgctgcgg atgaattcca tccacgcatt
     8641 cgccagcacg gatacggctc gatatcggga tcggcgggca agcgccaacg gcaagccgag
     8701 cagacatgcc gcggcaaatc cgagcagcgt cgctccgatc gtaatcggaa gcacccgcag
     8761 cagctgagga agaatctcca atgcgtagtc ccaactccac atgcgctata tcctcccctt
     8821 cacggccctg cgctccaaaa aacgaatgcc gagcgtcaag gtataggcca tgataaaata
     8881 tatcaggagc aataacgcaa aaatttgcgg cgtgctggag ttgtcgaagg agcgtaatac
     8941 catcccttgg taggtcaaat ccattaatgt gatgaggtaa acaagcgatg tccccttcag
     9001 cagctcgata agcagattgc cgaacgaagg cagcatgagc gccatcgcct gcggcagaat
     9061 gatgtttctc atccggagcc acggactcat attaagcgcg atggccgctt catattggcc
     9121 cttcggcacg gacaggatcg aaccccgcac cacctcggaa ccatacgcgc catagttcag
     9181 gccaagcgca agcacggccg cggtcatcgc cggcatgcgg acgtcgagca gcataggcag
     9241 cgcaaaatat aaccagaaca gctgaaccag caaagaagtt ccccgaaaca cctctacgta
     9301 gaccccgctg acaaagcgga cgatgcggta cttggacagg cgagccaatc ccgccgcaaa
     9361 agcgcagaag aaggccagca tcatcgagaa gcatacaatt tgcagcgtga cccacgctcc
     9421 ttgcagcagc gcaggcgcat attcaagcat ggcggaagtc ctcatggctt cactcctgcg
     9481 acagccgttc cgccgtcata tctccaggca gctcctgctc cgtgaacccg aacggctcaa
     9541 gcagttctag cagcttgccc gactgcttca acgcttccag ctcttgattg aatgcttccc
     9601 ggaagtcggc atcttccttg cggaacgccg ccgcgccata gcctcttacg cttttgccgt
     9661 caatctcggg ctgctcgaaa tccatgaccc gctccagttg atcatccttc gtggagtcaa
     9721 gcatcgcctg caaggatggt cccgtcatcg tcaccgcatc cacacgaccc gcctgcagcg
     9781 ccgagatggc cgacggttga tccggcaccg ttacgatttg ttccttcgcg acacctgatt
     9841 tcgctaaata ttcgagctcg atggccccca ccatgacagc gaccttgaca tccttgctgc
     9901 gggcaatgtc ctcatagctg cgcagattgg atgggttccc cttcttgacc gcgagcgcct
     9961 cgccaatgct gtattcgggg ttggcgaagg ccacttcctt gctccgctcg ggattgatga
    10021 acattcccgc ggtgatgatg tcgaaccgct tcgccttcaa ccccggaatg agagaaccga
    10081 actccgtcag gacgccgttc atctcctcga tgcccatccg tgtcaatact tcccgggcca
    10141 cctccacggc ctcgcccgtc agttggccat ccggcgtggc gtacgcatac ggcttctcat
    10201 tcgcgaagcc tacggtaaca tatccttccc gcctcgcctt ctccaaggcc gtctcctgcc
    10261 cggacgatgt tccgccgttg ggttcggtcc ggctatcgcc aggttgcgaa gcgcagcttg
    10321 ccagcaggat ggccatgacg cagcataacg catacgaata ccatttcttc atccgacaac
    10381 ctcc
//
