LOCUS       NZ_CP089312            10453 bp    DNA     linear   CON 28-JAN-2024
DEFINITION  Streptomyces sp. GQFP chromosome.
ACCESSION   NZ_CP089312
VERSION     NZ_CP089312.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. GQFP
  ORGANISM  Streptomyces sp. GQFP
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8306813)
  AUTHORS   Zhu,J.
  TITLE     Complete Genome Sequence of Streptomyces lacrimifluminis strain
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8306813)
  AUTHORS   Zhu,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2021) School of Life Sciences and Biotechnology,
            Shanghai Jiao Tong University, Dongchuan Road No.800, Shanghai
            200240, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 4; canu v. 1.6
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 109.0x
            Sequencing Technology             :: Illumina HiSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/28/2024 04:27:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,499
            CDSs (total)                      :: 7,422
            Genes (coding)                    :: 7,228
            CDSs (with protein)               :: 7,228
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 69 of 194
            Pseudo Genes (incomplete)         :: 156 of 194
            Pseudo Genes (internal stop)      :: 7 of 194
            Pseudo Genes (multiple problems)  :: 35 of 194
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:04:16
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6816128
            Orig. end                         :: 6826581
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP089312.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10453
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="melanin"
                     /region_number="20"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..10453
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10453
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5453](+)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5453
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     gene            complement(481..1584)
                     /locus_tag="LUX31_RS30720"
                     /old_locus_tag="LUX31_30720"
     CDS             complement(481..1584)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019056483.1"
                     /locus_tag="LUX31_RS30720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LUX31_30720"
                     /product="DUF3344 domain-containing protein"
                     /protein_id="WP_235478559.1"
                     /transl_table=11
                     /translation="MRISPGLPLRRAMVGVFALAAICTPGGTATAEPGKEAARLAFAPR
                     YQALQHGGIVRAANTSISCRATLGTCAGVRRGEQLASGTGGAKAAVNGDFDMFYVDVDS
                     DPNTYNSSRAEVRVPQGGRVSYARLYWGGNLKVGEQKPPEDNGRVLIAEPGGEYKELLA
                     DSVVGHRVARGADAFQASADVTKLVRESGSGLYTVAQVNVAMGRSAVGAWGGWTLVVAY
                     EKAAEPLRHLAVWDGFDTLEAREGDVPLVRLAELGFPVGANGRAGLVAYNGDRGSPGDS
                     LTVSVAGRTTVLGDAANPRDDVLNSTIGGAASERVPAYANTLGYDSDVFELGTALRRGG
                     DQLAFRLVSQRDAAWAGALFVAVDARQ"
     misc_feature    complement(1604..1619)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 18.92"
                     /tool="antismash"
     misc_feature    1893..1909
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 22.0"
                     /tool="antismash"
     gene            1923..2162
                     /gene="chpG"
                     /locus_tag="LUX31_RS30725"
                     /old_locus_tag="LUX31_30725"
     CDS             1923..2162
                     /codon_start=1
                     /gene="chpG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007449696.1"
                     /locus_tag="LUX31_RS30725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LUX31_30725"
                     /product="chaplin ChpG"
                     /protein_id="WP_235478560.1"
                     /transl_table=11
                     /translation="MSRIAKGLALSSVAVAAVAGTAGVAAADSDAHGVAAHSPGVLSGN
                     VLQVPVHVPVNVCGNTINVIGLLNPAFGNTCVND"
     gene            2381..3148
                     /locus_tag="LUX31_RS30730"
                     /old_locus_tag="LUX31_30730"
     CDS             2381..3148
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006382606.1"
                     /locus_tag="LUX31_RS30730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LUX31_30730"
                     /product="hypothetical protein"
                     /protein_id="WP_235478561.1"
                     /transl_table=11
                     /translation="MRALPARRIASTALCATLLLGIGAPAALADDGDAARERGHAASQA
                     PVPNADALLAQVKSLGDLGGVVKPVTDLLNAVLKADNGQLPLDEATKLGDAVKAAIAEA
                     TAAAPAAPVTPPADVPATPALPTTPALPTTPALPTAPALPTTPSLPVYSTLPAPLTDGK
                     AVKGPLDIKGDALTALQKAVDTLLTAVTSGDVANVVPAVTGVVTSLVGLVAATLLGSGL
                     PAPDLAGLPSLPKLPAEVPQLPVPVPPLRLPAS"
     gene            3316..3606
                     /locus_tag="LUX31_RS30735"
                     /old_locus_tag="LUX31_30735"
     CDS             3316..3606
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020116485.1"
                     /locus_tag="LUX31_RS30735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LUX31_30735"
                     /product="hypothetical protein"
                     /protein_id="WP_235480118.1"
                     /transl_table=11
                     /translation="MKSLKAAAVVAGSLVIAGAAAPAFAYDTADLTPTSLNGAVNTLTK
                     GPLDVDQLQPLQHQSNALDTENKESVLSTVKGATTALNSNGGLLGGLPLQG"
     gene            3759..4256
                     /locus_tag="LUX31_RS30740"
                     /old_locus_tag="LUX31_30740"
     CDS             3759..4256
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020116484.1"
                     /locus_tag="LUX31_RS30740"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LUX31_30740"
                     /product="DUF5949 family protein"
                     /protein_id="WP_235478562.1"
                     /transl_table=11
                     /translation="MTSTSSETRPFRIADLGTLVVMAWSGDAPDGDMPYLLAYSLGDGE
                     GGPEASAVAVEQLLANTGLPVGGDIVDGSTRPSLPVSLLVEAGTAVVNMPQLNAQCTPP
                     PEWLTAVGERGFAYLVFTTRPWPEAQPGKTVEPEALAAFAGAEETLTAAAHVILPARSL
                     RS"
     gene            4409..4918
                     /locus_tag="LUX31_RS30745"
                     /old_locus_tag="LUX31_30745"
     CDS             4409..4918
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006378447.1"
                     /locus_tag="LUX31_RS30745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LUX31_30745"
                     /product="hypothetical protein"
                     /protein_id="WP_235478563.1"
                     /transl_table=11
                     /translation="MKSTTRTLAAAVLTGVAAAVGAAAPAVAADTVPVPVPLDGVEKSL
                     NVNLPKVGGELPLLMPGVPEGPRYVEGQMIPASALPQVPLGAALPGLSAETPLPQVLGK
                     GFDEAAVSLPAADLRTLTPGLSVETPLTAPNPEVFGLPTLKEPQAAVLTPLLQTAPTGA
                     LGLGSL"
     gene            5001..5453
                     /locus_tag="LUX31_RS30750"
                     /old_locus_tag="LUX31_30750"
     CDS             5001..5453
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006378445.1"
                     /locus_tag="LUX31_RS30750"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LUX31_30750"
                     /product="tyrosinase cofactor"
                     /protein_id="WP_235478564.1"
                     /sec_met_domain="melC (E-value: 1e-47, bitscore: 160.6,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFVSRVPRGPAGPPARSGTRRDALRALCVSALALAVVPVVAAAWP
                     RREPGDEASSFDETYRGRRLRGMRADAGHMTHAMSTPGGGRWHVTVDGRPLHLMRRADG
                     TYLSMVDHYRSYPTPLEAARAAVDELGPGRRLTVEGGGGGGRGVHA"
     gene            5437..6300
                     /locus_tag="LUX31_RS30755"
                     /old_locus_tag="LUX31_30755"
     CDS             5437..6300
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019325459.1"
                     /locus_tag="LUX31_RS30755"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LUX31_30755"
                     /product="tyrosinase family protein"
                     /protein_id="WP_235478565.1"
                     /transl_table=11
                     /translation="MAYTRKDVSTLTRSERRRFVGALMEIKRRGEYDEFVRTHIEYYVS
                     DGEDGLRTAHMAPSFLPWHRRFLLDLEGALQRVDPSVTLPYWDWTRDRTAAGVPWSEDL
                     LGGTGRASDQRVMSGPFAYDHGDWTIRESVTDGEFLTRDLGRVRQPLTLPTRQDLDVAL
                     DDPVYDVSPWNSTSAKGFRNRLEGWGSGRGPGSWRNHNRVHRWVGGHMVGGASVNDPVF
                     WLHHAFVDLCWSRWQRRHGSRYLPERPPGFGDAQRGRVVARHESLPPWDVTPDQLEEHS
                     QIYRYA"
     gene            complement(6374..6592)
                     /locus_tag="LUX31_RS30760"
                     /old_locus_tag="LUX31_30760"
     CDS             complement(6374..6592)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006380891.1"
                     /locus_tag="LUX31_RS30760"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LUX31_30760"
                     /product="chaplin"
                     /protein_id="WP_328285738.1"
                     /transl_table=11
                     /translation="MATADAGAQAEAVGSPGVLSGNVLQVPVHVPVNVCGNSISVIGLL
                     NPAFGNTCVNVSDDDHKGNPGPGGYGS"
     gene            6910..7095
                     /locus_tag="LUX31_RS30765"
                     /old_locus_tag="LUX31_30765"
     CDS             6910..7095
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="LUX31_RS30765"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="LUX31_30765"
                     /product="DUF2087 domain-containing protein"
                     /protein_id="WP_235478567.1"
                     /transl_table=11
                     /translation="MASDQAEQNERQQTQNVLRTFVGADGRLARLPEHFETGVEYPERL
                     VDERLRVWCEVDADAA"
     misc_feature    7238..7253
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 19.66"
                     /tool="antismash"
     misc_feature    7303..7316
                     /note="TFBS match to CatR, H2O2-responsive repressor,
                     confidence: medium, score: 18.49"
                     /tool="antismash"
     gene            7446..8657
                     /locus_tag="LUX31_RS30770"
                     /old_locus_tag="LUX31_30770"
     CDS             7446..8657
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006380889.1"
                     /locus_tag="LUX31_RS30770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LUX31_30770"
                     /product="ATP-binding protein"
                     /protein_id="WP_235478568.1"
                     /transl_table=11
                     /translation="MTSLIQDPLLWILLVVLVAAVVAVMRARRTNMALRRKSNDLQSAR
                     EAAQSDRDRLYAEYTQLNAQHAGDLAAVRKDAEEDTKAVLKSAMRTLQVLADEQQLIIE
                     KSQRRYGDDEQVLGDLMMIDHTNSQFGRRAQGIAVLCGGWLGRRESTASVYDVARSAQG
                     RIKHFDRVTINTQVNVSVASRAVEPVAVVLAELLANATNYSAPGTRVDINIQAVPTGVC
                     LIVDDAGLGMNTEEKARATVLLSPQAPVDITSLGDPPKFGFAVSGMLATRYGFKVSVDS
                     VSPYGGVRAVILLPENLLTTDVPEPEGGAAKAEPQEPEAPSAAPQRLTPVATPGGPPRP
                     IGTTAGGLPKRRRRGSPVAVVPSPQEPAEPEFNTEVTASRIGAFQRGTLLGRDTTTMEG
                     PQDQ"
     gene            8654..9055
                     /locus_tag="LUX31_RS30775"
                     /old_locus_tag="LUX31_30775"
     CDS             8654..9055
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007386761.1"
                     /locus_tag="LUX31_RS30775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LUX31_30775"
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="WP_105973205.1"
                     /transl_table=11
                     /translation="MNPDLSWVLNDVLQVRGARHAILVSADGLLLERSSDIERDEAETN
                     AAAMSSMQSLSRAVAPFVGSGRGLWKQTLIEYDGGWIFLIAAGSGAYLAVSAALDVDME
                     AMSFRMQQQVGALGKAMTTPPRQNAGADA"
     gene            9052..9432
                     /locus_tag="LUX31_RS30780"
                     /old_locus_tag="LUX31_30780"
     CDS             9052..9432
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003950005.1"
                     /locus_tag="LUX31_RS30780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LUX31_30780"
                     /product="DUF742 domain-containing protein"
                     /protein_id="WP_235478569.1"
                     /transl_table=11
                     /translation="MNAHGEEAPVTVTSDFVRSYVITGGRRLPTADDLSLHTLVTLAPE
                     RELPLGASPEVRAIWDLCGGGYLSVAEVAAHLTLPVGVARLLLTDLFEQGHLLRRAAPP
                     RAQSVDRGIIEKVLHGLESLYG"
     misc_feature    9322..9324
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            9407..10000
                     /locus_tag="LUX31_RS30785"
                     /old_locus_tag="LUX31_30785"
     CDS             9407..10000
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003967632.1"
                     /locus_tag="LUX31_RS30785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LUX31_30785"
                     /product="ATP/GTP-binding protein"
                     /protein_id="WP_235478570.1"
                     /transl_table=11
                     /translation="MDSNPSTAETIYVSDAVTTTAKILVVGHFAVGKTTYIGSLSEITP
                     LRTEEKMTQASAHVDDLKGAPDKVTTTVALDFGRLTLSEELVLYLFGTPGQQRFMQLWE
                     DMARGALGALLLVDPSRLAETFPVIDLVEKYGLEYAIAVNTFDPEGGEFDEDEIREALD
                     LLPDTPVVYCDARDPKSSARALIALVQHLLARAA"
     misc_feature    complement(10074..10087)
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 17.44"
                     /tool="antismash"
ORIGIN
        1 ccaacgcgcc ccccaccgct cccacttgag cgccagggcc gcggtggacc cgccgaccgc
       61 ctcgaacgac caggcgtgcg gctggaacac cgtcgccacc cggccccgta ccgccagccg
      121 ggcggcgagc ccggccttcg cgctgtgcgc gtgcacgaca tccggccgca ccgccccgat
      181 cagccgtccg agccgccgta cctccccggc gagcgagggg cccggggcgc gcgtcgcgtg
      241 ccaggggcgt acgccggcgc cgagcgcccg cagtcgcgcc gggagttcac cgccgtccgg
      301 gcaggcgacc gtgacgtcaa gaccggcggc gagctgtgcc cgcgccaggt ccgtgacgac
      361 ccgggccacc ccgccgtcca ccggctgggt gatgtgcagg acccggggcc gggggtcggg
      421 tgctgacagt tgcatgcgcg gtttcctcgc tcacgggtgg cggcgttgga cgcgcctcgg
      481 ctactgccgg gcgtcgacgg ccacgaaaag tgcgccggcc cacgccgcgt cccgctggga
      541 aacgagccgg aaggccagct ggtcaccacc gcgccgcagc gccgtaccca gttcgaacac
      601 gtcggagtcg tagccgaggg tgttcgcgta cgccggaacg cgctccgagg cggcgccgcc
      661 gatcgtggaa ttcagcacgt cgtcgcgggg gttggccgcg tccccgagca cggtcgtgcg
      721 ccccgcgacg gacaccgtca gggagtcgcc ggggctgccg cggtccccgt tgtaggcgac
      781 cagacccgcc cgcccgttcg cgcccaccgg gaaccccagt tcggccaacc ggaccaacgg
      841 aacgtcaccc tcacgggctt ccagcgtgtc gaacccgtcc cacacggcga ggtgccgcag
      901 cggctccgcg gccttctcgt acgccacgac cagcgtccag ccgccccacg cgccgaccgc
      961 cgacctcccc atcgccacat tgacctgcgc gaccgtgtac agccccgaac cgctctcccg
     1021 gaccagcttc gtgacatcgg cggaggcctg gaacgcgtcg gcaccgcgcg ccacccggtg
     1081 cccgacgacc gagtccgcga gcagctcctt gtactccccg ccgggctcgg cgatcagcac
     1141 ccgcccgttg tcctccggcg gcttctgctc accgaccttg agattgccgc cccagtacag
     1201 ccgcgcgtac gagacccgcc cgccctgcgg cacccggacc tccgcacggc tggagttgta
     1261 cgtgttcgga tcgctgtcga catcgacgta gaacatgtcg aagtcaccgt tgaccgccgc
     1321 cttcgcgccg ccggtcccgc tcgcgagctg ctcgcccctg cggacgcccg cgcaggtccc
     1381 gagggtggcg cggcagctga tggacgtgtt ggccgcgcgg acgatcccgc cgtgctggag
     1441 ggcttggtag cgcggggcga acgcgaggcg cgcggcctcc ttgccgggct cggccgtcgc
     1501 cgtacctccc ggagtgcaga tcgcggcgag ggcaaagacg cccaccatcg cacggcgcag
     1561 cggaagaccc ggggaaatac gcatgaccgg cgttgccctt tcggggaaaa ggtcgtggac
     1621 cgacggacag aaaatgtgga catcacttat gtgccgcatc cgctcccggt ctgtttcacg
     1681 cgacggattc cgggtggcgg tcgacctttt gggtggcttt ggggctgctg gcggaaggtg
     1741 agtcactcta gtcgctatgc gtatttattg gtgattcatg acgactgtgt cggaatcgtg
     1801 tgcccgggcg gcccgagaga tcaccccatt ggcggcacaa cccgggcgcg cgccacgcgt
     1861 tgacacggcg tcgggcatcc ccgcccggat gtttgtgaac aactcccaag gagcacttct
     1921 ccatgtcgcg tatcgcgaag ggcctcgccc tttcctccgt tgccgtcgcc gctgtggcgg
     1981 gtaccgccgg tgtcgccgcc gccgacagcg acgcgcacgg cgtcgctgcc cactccccgg
     2041 gcgtcctgtc gggcaacgtc ctgcaggttc cggtccacgt gccggtcaac gtctgcggta
     2101 acaccatcaa cgtcatcggt ctgctgaacc cggcgttcgg caacacctgc gtcaacgact
     2161 gacgtaagtc ggcgcggacc cttctgccgg ccgctctccc ttcaggggag ggcggccggt
     2221 tcgcgttccg ggcgttccct ccgaggcgtt cgctccgagg tgtttgctcc gaatggcggc
     2281 ggtgaatgcc ggaccggttg caggtggcaa cggtggatct gacggtggac acatctgtcc
     2341 ggcacgtcac ggctccaccg caccaccgaa aggaacttcc atgcgcgctc tgcccgcacg
     2401 gcgtatcgcg tccaccgctc tctgcgccac cctcctgctc ggaataggcg caccggccgc
     2461 cctggcggac gacggcgacg cggcccgcga gcgcggccac gcggcttccc aggctcccgt
     2521 ccccaacgcc gacgcgctgc tcgcgcaggt caagtcgctg ggcgacctgg gcggcgtggt
     2581 caaacccgtc accgatctgc tgaacgccgt cctcaaggcg gacaacggcc aactgccgct
     2641 ggacgaggcg acgaagctgg gcgacgcggt gaaggcggcc atcgccgagg ccacggcggc
     2701 tgccccggca gcaccggtca ctccgccggc ggacgtcccg gcgacgccgg cccttcccac
     2761 gactcctgcc ctgccgacca ctcccgccct gccgacggcc ccggcgcttc cgacgacgcc
     2821 ctcgctgccg gtctactcca cgcttccggc gcccctcacc gacggcaagg cggtcaaggg
     2881 gccgctcgac atcaagggcg acgcgctcac cgcgctgcag aaggcggtcg acacgctgct
     2941 cacggcggtc acgtccggcg atgtcgcgaa cgtcgtcccg gccgtcaccg gggtggtgac
     3001 gagcctcgtc ggtctcgtcg ccgccacgct gctcggcagc ggtctgccgg cgcccgatct
     3061 ggccggactg ccgtcgctgc ccaagctgcc ggcggaggtg ccgcagctgc cggtcccggt
     3121 accgcccctg cgactgccgg ccagctaggg gtctcgtccc ggccgtcgcg tcgacctcac
     3181 cggtcggcgc ggcggctgtt cttctgcccg ggcgccaatt ccttttgtgc gtcaatccaa
     3241 tttctgcata cagggtttcc gccgtgaccg gggctcgtta ggcacggcga cagaattccc
     3301 gaagaaagga acatgatgaa gtccctgaag gctgccgctg ttgttgccgg gtccctggtc
     3361 atcgccggag ctgccgcccc cgcgttcgcc tacgacaccg ccgacctcac gcccaccagc
     3421 ctcaacggcg ctgtgaacac gctcaccaag ggccccctcg acgtggacca gttgcagccg
     3481 ctccagcacc agtcgaacgc gctggacacc gagaacaagg agtcggtgct cagcacggtg
     3541 aagggcgcga cgaccgccct caactcgaac ggcgggctgc tcggcgggct ccccctccag
     3601 ggctgacgca cgtcttgaaa ggaccggttc cggcgcgcgg aatcggtcct tcgcgctgtt
     3661 cgagccccgt ccctctttcg tgtggatacc ggcgggaaat cccccggtcg agtgagaaca
     3721 cggccggccg cgccaattcc cgtcgatagg gttccgcggt gacctcaacc tcaagcgaaa
     3781 cgcgcccttt ccgcatcgcc gacctgggca cgctcgtcgt catggcctgg agcggtgacg
     3841 cccccgacgg cgacatgccc tacctcctcg cctactccct cggggacggc gagggcggtc
     3901 cggaggcctc ggccgtcgcc gtcgagcagc tcctcgccaa caccggtctg ccggtcggcg
     3961 gcgacatcgt cgacggctcg acccggccga gcctgccggt cagcctgctc gtcgaggccg
     4021 gtacggccgt cgtcaacatg ccgcagctca acgcccagtg caccccgccc ccggagtggc
     4081 tcaccgcggt cggtgaacgg ggcttcgcct acctggtgtt caccacccgc ccctggcccg
     4141 aggcccagcc cggcaagacc gtcgagccgg aggccctggc cgccttcgcg ggcgccgagg
     4201 agaccctcac cgccgcggcc catgtcatcc tcccggcccg cagcctgcgc agctgacggc
     4261 ctgagtccgg agcggcggac ggggccgctc tccgggctga tctgacgact aatgcaccac
     4321 tcgggggaat cttgagctga tgcggttctc aggatcgtta cgcggtacgg acgaactccc
     4381 ccgattcctg taaaggaccg tacttgccat gaagtcgacc actcgaaccc ttgccgccgc
     4441 cgtcctcacc ggcgtggcgg cggcggtggg cgccgcggcc cccgccgtcg cggccgacac
     4501 ggtccccgta cccgtaccgc tggacggcgt cgagaagtcc ctcaacgtga acctgcccaa
     4561 ggtcggcggc gaactgccgc tgctgatgcc gggcgtgccc gaggggccgc gctatgtcga
     4621 gggccagatg atcccggcca gcgcgctgcc gcaggtgccg ctcggtgccg cgctgcccgg
     4681 gctgtcggcc gagacgccgc tgccgcaggt gctcgggaag ggcttcgacg aggccgccgt
     4741 cagcctcccc gcggccgacc tgcggaccct gacccccggt ctgtccgtgg agaccccgct
     4801 gaccgcgccc aaccccgagg tgttcggcct gccgacgctc aaggagcccc aggccgccgt
     4861 actcaccccg ctgctgcaga cggccccgac gggcgccctg ggactcggct cgctgtagtc
     4921 cacaggctga cgaaccggac gcgctggcca ccgggcggtc gcatcaccga acgaacggaa
     4981 ccaggagaga ggcgagagtc ttgttcgtca gcagagtgcc ccggggcccg gcgggtcccc
     5041 ccgcccgctc cggaacgcgg cgggacgcgc tgcgcgccct gtgcgtgtcg gccctcgccc
     5101 tggccgtggt cccggtcgtc gccgccgcgt ggccccgacg ggagcccggc gacgaggcgt
     5161 cgtccttcga cgagacgtac cggggccgtc ggctgcgggg catgagggca gacgccggcc
     5221 acatgacgca cgccatgagc acgcccggcg gcgggcggtg gcacgtcacc gtggacgggc
     5281 ggcccctgca tctgatgcgg cgcgcggacg ggacgtatct gagcatggtc gaccactacc
     5341 gctcgtaccc cacgcccctg gaggcggccc gcgccgccgt cgacgagctg ggccccgggc
     5401 ggcggttgac ggtcgagggc gggggagggg gcggtcgtgg cgtacacgcg taaggacgtc
     5461 agcacgctga cgcggagcga gcggcggagg ttcgtcgggg cgctgatgga gatcaaacgg
     5521 cggggcgagt acgacgagtt cgtccgcacg cacatcgagt actacgtctc cgacggcgag
     5581 gacgggctgc gtacggcgca catggcgccc tccttcctgc cctggcaccg gcggttcctg
     5641 ctggacctgg agggggccct gcagcgcgtg gacccgtcgg tgacgttgcc gtactgggac
     5701 tggacccggg accgtacggc ggcgggggtg ccctggagcg aggatctgct cggcgggacc
     5761 gggcgggcct ccgaccagcg ggtcatgagc gggccgttcg cctacgacca cggcgactgg
     5821 accatcaggg agagcgtcac cgacggtgag ttcctcaccc gggacctcgg ccgcgtccgc
     5881 cagccgctca cgctgccgac ccggcaggat ctggacgtgg ccctcgacga tcccgtctac
     5941 gacgtgtcgc cgtggaactc gacgtccgcg aaggggttcc gcaacaggct cgaagggtgg
     6001 gggagcgggc gcggccccgg ttcctggcgc aaccacaacc gcgtccaccg gtgggtcggc
     6061 gggcacatgg tcggtggcgc gtctgtcaac gacccggtgt tctggctgca ccacgccttc
     6121 gtcgacctgt gctggtcccg ctggcagcgc cggcacggct cgcgctatct gcccgagcgg
     6181 ccgccggggt tcggcgacgc gcagcgcggc cgggtcgtcg cccggcacga gtcgctgccg
     6241 ccgtgggacg tgacaccgga tcagctggag gagcacagcc agatctatcg gtacgcgtga
     6301 cacgtaccgg aacatgcgcg agagccccga cggtggatcc gtcggggctc tcgcggtgtt
     6361 cgcgtacgac aggtcagctg ccgtagccgc cggggccggg gttgcccttg tggtcgtcgt
     6421 cgctgacgtt gacgcaggtg ttgccgaagg ccgggttcag cagaccgatc acgctgatcg
     6481 agttcccgca cacgttgacg ggtacgtgga ccgggacctg caggacgttg ccggacagga
     6541 cgccgggcga gccgacggcc tcggcctggg cacccgcgtc agccgtggcc ataccggcac
     6601 cgccgagcac cacggcaccg gtgccgagag cggcgacggc agccttcgcg atgcgagaca
     6661 tcacgttctc ctttggttcg atgagtgcgg gcgctcgact cgcgcccgca cttcccctac
     6721 aacgcgcccg ctcacgcctg gtaacggcgt cgggccgggc gtcacccttt tcgaagcgca
     6781 ggggttgtca gccgcgtgtc gccgccccgg ggtcgagtac gccggggacc agctcgccca
     6841 ggaccgttcg gggcgcgcgg ctgaaggggc tcacttccgc tcagtccaca gggacggtta
     6901 acctcaggca tggcatccga ccaggccgag cagaacgagc ggcagcagac ccagaacgtg
     6961 ttgcggacct tcgtaggggc ggacggtcgt ctcgcgcggc tccccgagca cttcgagacc
     7021 ggcgtggagt atccggagcg gctcgtcgac gagcggctcc gcgtgtggtg cgaggtggac
     7081 gccgacgcag cctgagcgct tcaacaccct tggcccgctt cccggatgac gggggagcgg
     7141 gcctttccgt gcccgaacgg tatcgggtcc ataactgtcg tggacccgtt cgcgaatgct
     7201 gtgtgaaggt ctccaagtga gcgtaagcgg aacctttttg acgctctctt gactgctaag
     7261 atcatcccac tttcagtagg ctgaggcgtg ccgcgcgcac gggggacagt ctctaagggc
     7321 cccatcgcga aggagtggct gagttggtcc gccggtaagg ttcgccaccg ggctgggccc
     7381 gtgttccagg ccggacccat cggactgcaa ctgctcctgt cgcaaggcga cttgaagaga
     7441 gtctcatgac gtcattgatc caggatccac tgctgtggat cttgctcgtg gtcctggtcg
     7501 ctgcggtcgt cgcagtgatg cgggcccgca gaaccaacat ggccctccgc aggaagagca
     7561 acgacctgca gagcgcccgc gaagccgcgc agagcgaccg cgaccgcctc tatgcggaat
     7621 acacccagct gaacgcacag cacgccggcg acctcgccgc ggtgcgcaag gatgcggagg
     7681 aagacaccaa ggccgtcctg aagtccgcga tgcggacgct ccaggtcctc gccgacgaac
     7741 agcagctcat catcgagaag tcgcagcgcc gctacggcga cgacgaacag gtcctcggcg
     7801 acctgatgat gatcgaccac accaacagcc agttcggccg ccgcgcccag ggcatcgcgg
     7861 tgctctgcgg cggctggctc ggccggcgcg agtccaccgc ctccgtgtac gacgtggccc
     7921 gcagtgccca gggccgcatc aagcacttcg accgcgtcac catcaacacg caggtgaacg
     7981 tctcggtggc cagtcgggcc gtcgagcccg tcgcggtcgt cctcgccgaa ctgctcgcca
     8041 acgccaccaa ctacagcgcg cccggcaccc gcgtcgacat caacatccag gccgtaccca
     8101 ccggggtctg cctgatcgtc gacgacgccg gcctcgggat gaacaccgag gagaaggcgc
     8161 gggccaccgt cctgctctcc ccgcaggcgc cggtcgacat cacgagcctc ggcgacccgc
     8221 cgaagttcgg cttcgccgtg tccgggatgc tcgccaccag gtacggcttc aaggtctcgg
     8281 tcgactcggt gtccccctac ggcggcgtac gggcggtgat tctcttgcct gagaacctgt
     8341 tgacgacgga cgttcccgag cccgagggcg gtgccgccaa ggccgaaccc caggagcccg
     8401 aggctccttc ggccgccccg cagcggctga ccccggtggc cacgcccggc ggaccgccgc
     8461 gtccgatcgg gacgacggcg ggcggcctgc ccaagcggcg gcggcgcggc agcccggtcg
     8521 cggtggtgcc ctcgcctcag gagccggccg agccggagtt caacacagag gtgacggcct
     8581 cacgtatcgg tgcgttccag cgcggaacgc tgcttgggcg ggatacgacc accatggaag
     8641 gaccacagga ccagtgaatc ccgatctgtc gtgggtgctg aacgatgtgc tgcaagtccg
     8701 cggcgcccgt cacgcgatcc tcgtatcggc cgacggcctc ctgctggagc ggtccagtga
     8761 catcgagcgc gacgaggcgg agaccaacgc cgccgcgatg agctcgatgc agtccctcag
     8821 ccgggccgtc gcccccttcg tcggctccgg acgcggtctg tggaagcaga ccctgatcga
     8881 gtacgacggc ggctggatct tcctgatcgc cgcgggcagc ggtgcctacc tcgccgtgtc
     8941 ggccgcgctg gacgtcgaca tggaggccat gtccttccgg atgcagcagc aggtgggcgc
     9001 gctcggcaag gcgatgacca ccccgccgcg tcagaacgcc ggtgcggacg catgaacgcc
     9061 cacggcgagg aagctccggt cacggtcacc agcgacttcg tccgctcgta cgtcatcacg
     9121 ggcgggcggc ggctgccgac cgccgatgac ctgtccctgc acaccctggt caccctggct
     9181 cccgaacggg aactgccgct cggcgccagc cccgaggtac gggccatctg ggacctgtgc
     9241 ggcggcggct acctctccgt cgcggaggtc gccgcgcacc tgacgctccc ggtgggcgtc
     9301 gcccggctcc tgctgaccga tttattcgaa cagggtcatc tcctgcgccg tgccgcgccc
     9361 ccacgcgccc agtccgtcga cagaggaatc atcgaaaagg tgctgcatgg actcgaatcc
     9421 ctctacggct gagacgatct acgtctcaga cgcggtgacc accaccgcga agatccttgt
     9481 cgtagggcac ttcgcggtgg gcaagaccac gtacatcggc tcgctgtcgg agatcacccc
     9541 gctgcgcacc gaggagaaga tgacgcaggc gtccgcccac gtggacgacc tcaagggcgc
     9601 gccggacaag gtgacgacca cggtcgcgct ggacttcggc cggctcacgc tgagcgagga
     9661 actggtgctg tacctgttcg gtacgcccgg tcagcagcgc ttcatgcagc tgtgggagga
     9721 catggcccgc ggcgcgctcg gagccctgct cctggtcgac ccgtcccggc tggccgaaac
     9781 gtttccggtg atcgacctgg tcgagaagta cggcctggag tacgccatcg ccgtcaacac
     9841 cttcgacccg gagggcggcg agttcgacga ggacgagatc cgcgaggccc tcgacctgct
     9901 gcccgacacc ccggtcgtct actgcgacgc ccgggacccg aagtcgtccg cgagagcgct
     9961 gatcgccctc gtgcagcacc tgctcgcccg cgcggcctga gcacagggct ccgcacaagg
    10021 tttttcctca cccccgtccc ccgcccccga ggcgtgggga acccaaagga acctccatgg
    10081 acatacagca cgcgggtgcc acgccgagcg ctcccgccag atgccccatg cacggcgccg
    10141 acttcgccgc cgacccgcag ggcagctacg acaagttgcg cgcccacggc ccggcggcac
    10201 ccgtcgagct ggcaccgggc gtggacgcca cgctcgtcgt ccagcacgag atggccctca
    10261 gggtgctcca gaacaccacc ctgttcgccc gtgactcgcg ccgctgggcc gccctgaacg
    10321 agggccgcat ctccctggac agccccgtcc tgccgatgat ggcgtaccgg cccaacgtcc
    10381 tgttcaccga cggcgcggtg catctgcggc tgcgcaaggc cgtcaccgag agcctcgcca
    10441 agctgaacat cag
//
