LOCUS       NZ_CP054019            21050 bp    DNA     linear   CON 28-JAN-2024
DEFINITION  Streptomyces sp. A1-5 chromosome.
ACCESSION   NZ_CP054019
VERSION     NZ_CP054019.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. A1-5
  ORGANISM  Streptomyces sp. A1-5
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 9459690)
  AUTHORS   Dashti,Y., Sumang,F.A.M., de los Santos,E.L.C., Alcantara,E.P. and
            Challis,G.L.
  TITLE     Structural revision and biosynthesis of polyketides FXJ15321 and
            FXJ15322 from Streptomyces sp. A1-5
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9459690)
  AUTHORS   de los Santos,E.L.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2020) Warwick Integrative Synthetic Biology
            Centre, University of Warwick, Gibbet Hill Road, Coventry CV4 7AL,
            United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: JUN-2019
            Assembly Method                   :: Flye v. 2.4.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Oxford Nanopore MiniION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/28/2024 04:28:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,328
            CDSs (total)                      :: 8,230
            Genes (coding)                    :: 7,847
            CDSs (with protein)               :: 7,847
            Genes (RNA)                       :: 98
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 156 of 383
            Pseudo Genes (incomplete)         :: 280 of 383
            Pseudo Genes (internal stop)      :: 26 of 383
            Pseudo Genes (multiple problems)  :: 73 of 383
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 22:08:01
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 8530387
            Orig. end                         :: 8551437
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP054019.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Philippine National
            Collection of Microorganisms.
FEATURES             Location/Qualifiers
     region          1..21050
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="atropopeptide"
                     /region_number="35"
                     /rules="atropopeptide_p450"
                     /tool="antismash"
     cand_cluster    1..21050
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="atropopeptide_p450"
                     /kind="single"
                     /product="atropopeptide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..21050
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:11050](+)"
                     /cutoff="10000"
                     /detection_rule="atropopeptide_p450"
                     /neighbourhood="10000"
                     /product="atropopeptide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..11050
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="atropopeptide_p450"
                     /neighbourhood="10000"
                     /product="atropopeptide"
                     /protocluster_number="1"
     gene            complement(1954..2898)
                     /locus_tag="HRD51_RS37720"
                     /old_locus_tag="HRD51_37235"
     CDS             complement(1954..2898)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1158: ornithine cyclodeaminase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018537613.1"
                     /locus_tag="HRD51_RS37720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HRD51_37235"
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="WP_235095380.1"
                     /transl_table=11
                     /translation="MSATPPVPHLPRTIDETGLLRALPMAAAFDALRAALLDGPDPEAD
                     PPRTIVPVEHGQLLLMPAHRSRYAGVKIATVAPDNPAAGLPRVQGQYLLFDAPTLAPLA
                     LLDGIALTTVRTAAVSALAADLLAVPEASHLVVFGTGPQARGHLDALRAVRPLTHVTVV
                     GRTPANVERFVATARRDTGLRVEAGEPSAVAGTDLVACCTTARAPLFDGALLPPHAMVT
                     AVGSHERGSREVDGTTVLRSTVVVESRSAAAREAGDLIPELESGRLDREALVTLAGLAT
                     GAAAVDPDRPRLFKSVGMAWEDLAVAGAAYEAT"
     gene            complement(3007..4254)
                     /locus_tag="HRD51_RS37725"
                     /old_locus_tag="HRD51_37240"
     CDS             complement(3007..4254)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014142647.1"
                     /locus_tag="HRD51_RS37725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HRD51_37240"
                     /product="chitinase"
                     /protein_id="WP_235095381.1"
                     /transl_table=11
                     /translation="MPPRHVRTLAAAVSAACIALGVAGTALATGQRAAPRPAAATPAPK
                     ALGGNWYAAAPYLMPLDNDPPDAGAVMDATGLKAFQLAFVLAPNGGGCSPTWDGLHPVA
                     SDTAVAGVISAIRAKGGDVSASIGGYNGTKLGQACGTPEATAAAYQQVLDKYGLKALDF
                     DLEEPEYENSAAIHNEIGAAKILQQQNPGLYLSVTTAGTADGTGWFGKQMLNEAKSQSF
                     VPDNFSIMPFDGGFNGAAAQTSALTNFNAVLRSTFGWDEATAYAHEGISQMNGRSDTGE
                     FFTQADFQTVLDYATSHHMARFTNWSVNRDRQCTPPDNNGRTSGTCSSVPQGAWDFTKY
                     SAKFAGATPPTTPPTTPPTTPPGGSCAASPYATGTVYVGGDTVSYNGHTWKARWWTQGE
                     APSTGGSGVWQDLGTC"
     misc_feature    complement(4279..4294)
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: strong, score: 24.4"
                     /tool="antismash"
     gene            4419..5039
                     /locus_tag="HRD51_RS37730"
                     /old_locus_tag="HRD51_37245"
     CDS             4419..5039
                     /EC_number="2.7.7.87"
                     /GO_function="GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA]"
                     /GO_process="GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004004059.1"
                     /locus_tag="HRD51_RS37730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HRD51_37245"
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="WP_102924992.1"
                     /transl_table=11
                     /translation="MAKYFDVHPDNPQRRTITTVADTVRSGGLIAYPTDSCFALGCRLG
                     SRDGIDRIRSIRALDDRHHFTLVCQNFSQLGQFVQIDNDVFRAIKAATPGRYTFILPAT
                     KEVPRQLLHPKKKTVGVRIPDHTVTQALLEELGEPLLSSTLLLPDEDEPLTQGWEIKER
                     LDHVVDAVVDSGDCGTEPTTVVDFSGGVVEIVRRGAGDASRFE"
     gene            5054..5605
                     /locus_tag="HRD51_RS37735"
                     /old_locus_tag="HRD51_37250"
     CDS             5054..5605
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018537610.1"
                     /locus_tag="HRD51_RS37735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HRD51_37250"
                     /product="hypothetical protein"
                     /protein_id="WP_205368226.1"
                     /transl_table=11
                     /translation="MAAKRNRAIPALAVALALQAGVAGVATAQAAPAVASAPAAAPAAA
                     IRITEGQVGEVGPGRTIVYPAVTSCLTVTVRLADGGKVGAHASLFQVPGEYRSDRILAA
                     VQQRIGTRAVRAVEVRGAVGAWHPSYFTRAIESYPEGAQIPVPTAPDPDGLARAVADGL
                     GQPRGKVTVQDLPDGDQTVE"
     gene            5979..7781
                     /locus_tag="HRD51_RS37740"
                     /old_locus_tag="HRD51_37255"
     CDS             5979..7781
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018537609.1"
                     /locus_tag="HRD51_RS37740"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HRD51_37255"
                     /product="coagulation factor 5/8 type domain-containing
                     protein"
                     /protein_id="WP_235095382.1"
                     /transl_table=11
                     /translation="MTPASKEPPTPERPTVGRRTVLGAAAAVASAPALLGPARTAHATP
                     PHRTPGALPGGGDLGPNVLVFDPATPGIQAKLDEVFKQQEKAQFDTGRYALLFKPGTYH
                     GLNAQLGFYTSIAGLGLSPDDTTINGDVTVDAGWFNGNATQNFWRSAENLALVPVSGTN
                     RWAVAQAAPFRRMHVRGGLNLAPAGDGWASGGYLADSRIDGTVEPRSQQQWYARDSAVG
                     GWSNGVWNMVFSGVQGAPGQTFPNPPYTVLDTTPLSREKPFLHLDGADYRVFLPELRTN
                     ASGVTWGNGTPRGTSLPLSQFYVAKPGVSAATLNQALAQGLHLLLTPGIYHLDQPIQVT
                     APNTVVLGLGYATLIPDNGVTAVRTADVDGIRLAGFLIDAGPVNSATLLEIGPAGAAAD
                     HSANPTTVQDVFLRIGGAGPGKATTSIVVNSRHTIIDHTWVWRADHGDGVGWDTNRADY
                     GIRVNGDDVLATGLFVEHFNKYDVQWYGQRGRTVFLQNEKAYDAPDQAAVQDGDVKGYA
                     AYKVADSVTSHEAWGLGSYCYYNVDPSIVQHHGFAAPNAPGVRFHDLLVVSLGGKGQFE
                     RVINDTGAPTSGSSTVPSTVVAYP"
     gene            complement(7902..8309)
                     /locus_tag="HRD51_RS37745"
                     /old_locus_tag="HRD51_37260"
     CDS             complement(7902..8309)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016575932.1"
                     /locus_tag="HRD51_RS37745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HRD51_37260"
                     /product="DUF3224 domain-containing protein"
                     /protein_id="WP_102924989.1"
                     /transl_table=11
                     /translation="MPAQTTGRISYANWAEQPIGSDATSPRLARACVTNNFSGGIEADR
                     TACEYTIVYTSDKTGTFTGLELLTGRLDGREGSFVLEERGAFDADGTVRCTFEVVPGSA
                     TGDLVGLSGSGSFRYEAGNPSFPYTFDYRMG"
     gene            8412..9395
                     /locus_tag="HRD51_RS37750"
                     /old_locus_tag="HRD51_37265"
     CDS             8412..9395
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016575931.1"
                     /locus_tag="HRD51_RS37750"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HRD51_37265"
                     /product="WYL domain-containing protein"
                     /protein_id="WP_235095383.1"
                     /transl_table=11
                     /translation="MRADRLLALLLLLQNRGRMTAAELAAELEVSVRTIYRDIEALGAS
                     GVPVRTERGPDGGYRLMDGYRTRLTGLTGAEADALFFAGAPGPARDLGLGAVLATAQLK
                     VQAALPVELAERTRRIQDRFHLDAPAWFRDADPVAHLAPVARAVWEQRVVRTHYRCWRG
                     EVHRELRPLGIVLKGGIWYVVALADEAVRTYRIARFQAVELTDEAFARPAGFDLAAYWA
                     ESSRRLAAALHQDTARLRISPQARRLLPMLYGAVGRRALEGAGEPAADGWTEVTMPVEG
                     PAVAVGDLLRLGAEAEVLGPPALRRAVAEAVTVLAGRYATADDPSE"
     gene            complement(9434..9571)
                     /locus_tag="HRD51_RS37755"
                     /old_locus_tag="HRD51_37270"
     CDS             complement(9434..9571)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="HRD51_RS37755"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="HRD51_37270"
                     /product="hypothetical protein"
                     /protein_id="WP_235095384.1"
                     /transl_table=11
                     /translation="MILRSRAARPPRGAGAGRTRNPGFDTTLHTFRQGTGRKIQLSPQG
                     "
     misc_feature    complement(9563..9565)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            10001..11050
                     /locus_tag="HRD51_RS37760"
                     /old_locus_tag="HRD51_37275"
     CDS             10001..11050
                     /GO_function="GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /GO_function="GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /GO_function="GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     atropopeptide: atropopeptide_p450"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1034: cytochrome P450"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014145092.1"
                     /locus_tag="HRD51_RS37760"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HRD51_37275"
                     /product="cytochrome P450"
                     /protein_id="WP_235095385.1"
                     /sec_met_domain="atropopeptide_p450 (E-value: 2e-176,
                     bitscore: 585.1, seeds: 287, tool: rule-based-clusters)"
                     /sec_met_domain="p450 (E-value: 2.8e-11, bitscore: 41.8,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSQRPTVTRGDRRVRFAPRIDALLHRHTGQELFRLEPTTIGVAGA
                     DLMDALLRSRPANAEERPTFKPVLGRHVGRADAAAYMQALGADVRKALQRPLETFVDLT
                     GVWPAVPHAYLRDLVFGQELGRLRILVDRRLELTPKLTWSAVASGAALLRRPDPAEPLS
                     KLATLALGAAGYADRRYALYLYRRVAAPICFTVAALVTNAVWLGAPFDDAVPNRQLLAE
                     ALRLLPPSWNILRMASPELTAVDDRIGPTDDVLLLPLLSHRDPALWEDPGAFRPERWAD
                     LDPDNHPGYLPFGHADERCWARHMVLPLAERILDLVRRDGLTVRSAQTVGRVELDGLME
                     VAEVRVTRG"
     gene            complement(11105..11434)
                     /locus_tag="HRD51_RS37765"
                     /old_locus_tag="HRD51_37280"
     CDS             complement(11105..11434)
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1199: NmrA family
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="HRD51_RS37765"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="HRD51_37280"
                     /product="hypothetical protein"
                     /protein_id="WP_235095386.1"
                     /transl_table=11
                     /translation="MAAHALTDPVPHNTDWVLTGPAALSYDAVAAILTEVSGRPVRHRA
                     VSEAELGTRLARHLPADFAAILADLDRRIAEGAEDRVTDAVTRVTGRPPRSFAAYVRDN
                     VQSLT"
     gene            complement(11491..11952)
                     /locus_tag="HRD51_RS37770"
                     /old_locus_tag="HRD51_37285"
     CDS             complement(11491..11952)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018385579.1"
                     /locus_tag="HRD51_RS37770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HRD51_37285"
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="WP_235096203.1"
                     /transl_table=11
                     /translation="MEEKRDAPATTAHRTPEDLFRHGPRLLLDQDFAAWVDLWDDQGVF
                     EFPFAPEGRPRRLEGRSAVAASMRDYPHHIELHDVPHLTIHQTQAPGTIIAEMSAVGRL
                     VATDEPFAMSYVAVVAVANGRIAHYRDYGNPLAVPATTRTRTPSALTAC"
     gene            12049..12636
                     /locus_tag="HRD51_RS37775"
                     /old_locus_tag="HRD51_37290"
     CDS             12049..12636
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016639027.1"
                     /locus_tag="HRD51_RS37775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HRD51_37290"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_205368211.1"
                     /transl_table=11
                     /translation="MPEPRQRADAVRSREAVLDAAEALFARSGPAEVSMNAVASAAGVG
                     KGTVFRAFTDRAGLLQALAERRTAALRRAVAEGPAPLGPGAPPHERIPAVLDALARFKL
                     DNQSLYLVLEEAGAASPYRSPSYTWWHTTLRDSLAQCADAGRADFVAHALLAAVRADLI
                     AHLLTVEGLSVDEIRDRLAVYVRSVLPRDTHA"
     gene            complement(12696..13595)
                     /locus_tag="HRD51_RS37780"
                     /old_locus_tag="HRD51_37295"
     CDS             complement(12696..13595)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018543082.1"
                     /locus_tag="HRD51_RS37780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HRD51_37295"
                     /product="RNA polymerase sigma factor SigF"
                     /protein_id="WP_235095387.1"
                     /transl_table=11
                     /translation="MTTAVSTHTGTRHGQRQVQGHRTVSPGTGGLPYIAEASKMAPKDA
                     KALSRPFFERLAVLEEGTHAYQYVRNTLVEMNLSLVRYAAARFRNRGGDPSEMEDIVQV
                     GTIGLIKAIDRFDLSREVEFTTFAVPYIVGEIKRFFRDATWAVHVPRRLQELRIDIAQA
                     KERLFARLDRDPTVAELAEHLCLGEDEVIDGLIAGNGYTAGSLDQPSDPAEDESARSLA
                     DALGEEDPRLQLAEDLHALTPLVRKLDRRDRRILTLRFVHEKTQSEIGEELGVSQMHVS
                     RLLARILGRLRTGLIGQP"
     misc_feature    complement(13655..13664)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 13.85"
                     /tool="antismash"
     misc_feature    13674..13683
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 13.63"
                     /tool="antismash"
     gene            complement(13879..14334)
                     /locus_tag="HRD51_RS37785"
                     /old_locus_tag="HRD51_37300"
     CDS             complement(13879..14334)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016577361.1"
                     /locus_tag="HRD51_RS37785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HRD51_37300"
                     /product="SRPBCC domain-containing protein"
                     /protein_id="WP_235095388.1"
                     /transl_table=11
                     /translation="MSNEPMRLPDLSSRPHQLAVERLMSGTPAALYRAWTEQFDRWFAA
                     PGSVLMEGRVNTAFFFETEHEGARHPHYGRFLRLDPDRLVELTWVTGAGGTQGAETVVT
                     VAFAAEGSGTQLRLSHAGFADEAARDRHRDAWPHVLEQLDRRIGAAH"
     gene            14527..15630
                     /locus_tag="HRD51_RS37790"
                     /old_locus_tag="HRD51_37305"
     CDS             14527..15630
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="HRD51_RS37790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="HRD51_37305"
                     /product="hypothetical protein"
                     /protein_id="WP_235095389.1"
                     /transl_table=11
                     /translation="MDFYGELALTGDGAPTVEMSALWPFRRMASRPDRSVRVGRMSEEI
                     AALVRRLASESREDRAAAQEALTAVGAPVVKHLLPLLRNDETPGSSAAESCLESLGDAA
                     IDPLREVRAEGPGSLRKAALRMLATIGGADALSDADRAAVERLVQIKLPDEEPGDLPAD
                     CWIAVPEAEIGDIVRALDLHDVRPVTTAMGLSAAVEHENSLRRPTEDGGVTTAYRVFIT
                     PEFDGWRLIYGVDYLNDNWAQIVTRLSARCREAHFYAIDDYNGAQVWWVSENGHDKRGY
                     RTYGDPEWIGEPMEFERNLMPGGDAYDDEDDDAEQYAEGVRDPEEVASSISIQPTVVEV
                     LDRVGHGWLAVTDPVVGHGRFRGALDI"
     gene            complement(15706..20940)
                     /locus_tag="HRD51_RS37795"
                     /old_locus_tag="HRD51_37310"
     CDS             complement(15706..20940)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF026980.4"
                     /locus_tag="HRD51_RS37795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HRD51_37310"
                     /product="toxin glutamine deamidase domain-containing
                     protein"
                     /protein_id="WP_235095390.1"
                     /transl_table=11
                     /translation="MLPDALEWVLEMLGFNWPTADEDKLQESAQQWRNFASGVEELQQR
                     GVTAAGNVVSQNSGDSIDGFNTTWEKFNGGSGSGYFADARTAAETIAVALDVAAGLVIG
                     MKVAVIAQLAILAAEIIAAQAAAPFTFGLSEIGVAAATAATRAIVRKIIKEAAKQVLDA
                     ALETAKEPAVSALQAMISDVIAQSVNMSFGAQNGFDVGRTVNKGAEEGVNALKNSGQTF
                     TEALRDGAGAKAGHHVRNGLESAAGHNSGGDSGGGSGHESAGGGSGGGNTSSGSDTSGG
                     GQSSPGGNHSPSSGSNSPSDGGSSSPGGGGSPSGGDGSSSTDGAPAGGSSAGGTSAGAS
                     SAGGGATASGGSSSAGHSTPTNNSSTPPNSSPDGGSSAADGGSSSSAGGSGHSPDGGNS
                     PRPAHSAAPPPSQLTPFDAGYREAQAASPTPDASSSATSSPSSSDSSARPNTDTPTSHD
                     TSNNDSNTDSSTNTNTAPRPDSAAPTPEHHSNGIGDPNGGSGNTGGVGTTGGSVTNSGN
                     DGSTDTSTHTGPQPQHAPSPVPDPSPSPSPSPSPSPTPDHSGTQHDDGNSSSNGGGSRP
                     SMPTGGGVASGGGAPHAAPSGHGYSGPSSSGAPHQPDPNRPQDDGPVTVHTESATLAPP
                     PPGSAQPSHAGGETPGSAAAPPPSTQQSPQSGVVMGGGTMPAGAPAGTPVGGSPSATTP
                     PRSTAPSSPAGNGGAPPRGTPTRDPRNPTAADQSSRPRPTPARSTAQPPRTAQPPRTER
                     PHGEQPSPRPSRPAQQPPHTSDAPPSPTTPDHDSQSPNTNHDPKPDSESRPKDDQAPDN
                     KDDTSHTPNPSDPDDVSDVSDVSDTSDAPDASDATATPDDRPSLDEIRSGIQESPGGLL
                     PPDTVDQQALENAVPRNDDGSPQRFPDPTAGWAQLQNDGGTNVPGRSNNCADCSRSFLE
                     TWYGNPQVSAPRTPDQTPDGTPDHWSPESKANENIIDWAGAPHSYAGTSPDGHHAIAQE
                     LLKAGPGSAAIVQVNWADGGGHAFNAVNHNGKIVWVDTQSGEVSHHPINTEGATDVFHI
                     PLDADRKPLHPAPDASNGSGATDSHHKSDGTDSDHTAHADQSPPAANGSHDSGDGTARR
                     GAPIPDHGKDERPSAADPAALKRKASEQPSPDGESVSSPSESGSDSHRRTRIKVGGEGQ
                     PGQQSGIDPHTDTPSPPQATLGSLPDHMDALGLDDHRMDVDDDHTQQHSPPQQAPQTQG
                     TNTGSGTAEHPTSGPPYGKPTPDTPMDLDGQPPAGPSNSGGGNASEQDHGQSTGSTADP
                     KKDNGNNTDGGKGDNKTDNKTDGKTDGKTDGKTDGKTDGKEPYSDPRDRGTSDTSKQEK
                     EGKHTLDAEKKDSKEYGKVPDKLQAQLRNDRDVHRIPLDRVHDRLTGWADDGSLARVLR
                     ASSGHPQTNGPDAGKGPQKFTQSDLEKRLPGFKDLDRGEKLAVVSSLTRLSVGFHEQHG
                     VGSNPVDVDKPYRPPNEPDAKDGTTDSAAKNSDESLGVRGHRNSGNKYLDKLKHDGPMP
                     ESLKQNSPDFTDRNYAVLEVEGPPPARETHYVADSSVPVGEKYVSGRHSEKHLMEWLNR
                     ANQEGAQYSAKALYTEREPCGNGQGHAKCSNVLREMTPKDTKVYYSTTYRTDPEDVKAK
                     KQLDTEKKKLKKEFDKKPIAGVQKEINDRLDRREGKSANWIAAEKDKIDKLSEAEQRKK
                     LSDMIDAEFTKRKNGMTTPEKQAMVREMDRHIAHLDSTWKKIQPSLL"
     misc_feature    complement(20866..20868)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
ORIGIN
        1 gcggctgggt ggagtcgcgc aggtcgttca gcgcggtgtc caggtcgcgc gccagggtca
       61 gcaggtccgc cgtcggcccg ccggacagct gggccaccgc cgccgcaacc gcctcccgca
      121 accgttccag gacggcgccc agatgctcgt cggtgcgccg gtcggtgtgc accggcagga
      181 tgaccgccgc ggcgatgatc ccgcacgccg cgcccagcgc ggtctcctcg atgcgcagca
      241 ccagcgtcgc gacgctgtag gtgttcagga cggtgtagag caggccgagc atcgcggtga
      301 cgaagaagga catcagcgcg tacgacagcg gcgcggtgaa gaacatgccg aagacgcaca
      361 acagcaccag cgcgaacgcc gtccaggtgt ggttgcccac cagccccgcc agcgccacac
      421 cggcgatcac gcccagcacc gtgccgacca gccggcggta ccccttgacc aggacctcgc
      481 cggtggacgc ggtgttgagg aacaccaccc agcaggtcag caccgcccag taccagcgct
      541 gggtggacag gaactccccg ccgacgatgg ccagcgccga gcccaccgcg acctggaacg
      601 cggcccgggt ggtgggccgc tggagcccgg tgtgctcgtc ctcctcggcc tcctcggcac
      661 cgacgatcgc caggtcctcg gcgtcgaact cctcccgcga gcggatcgtg gcggagctgt
      721 cgtcggactc gtcctgcggc ccgtccagcg ccagccgcag acccagcacg gcgcgggcgg
      781 cctcaccgag cgcgcggaag gcgtcctgga cggcgcccga gccgcgcggc aggttgtcct
      841 cgtcgcggta gccgagcagc cggttgcgca cgtgggccag cgcggtgccg cggtcgtcgg
      901 gcgcccggcg ggccatcagc agcgccaggg cctccaggtc ccggcggagc gtggccacgg
      961 catcgccctc ggactgcatg cggttcgcgg tggccggtac cggggcgtgc ggcaggtgca
     1021 gggtgagggt gtcggtgcgc tccgcgctgc gcgcgttgag cagcgtcatc cccaggcgtt
     1081 cggcggcgat ctcggcgtcc gcgatgcggc gctggagcag cgccgcggtg gtcgcgtcgc
     1141 gggtgccctc ctccaggcgg ccctggatca tcagcgccgt ctcgtgcagc cgggcggtgt
     1201 gccgccgcag gtcgtcgagg acgccgtcca actcctcggg cccggcgtcc accaactccc
     1261 ggtgggtggc caccagctgt gccaggcggg cccggaacgc ctcccgcagt cggctcaggg
     1321 tgcgttcggg ggtgtccggg aaggcggcga agcgggccac ggcgctgcac gcgaacgcga
     1381 cggccagggt caggaacagc tccggcagcg tggccacggt ggcgtgcacg aacagcgaga
     1441 tgaagtagac ctgaaagccg atcagaccca gcgccgtgcc gcggtcgccg aaccggcggg
     1501 cgtacgcggc gccgaagatc agcgcgatga agaacaggtc accggcgatg atccgactgt
     1561 gcagcagcgc ccccagcgcc accgcggcca acgccaccgg caggccgagg gcgagggtga
     1621 cggcctggcc gcgcacctcc ttctcccgga tggcgaaggt ggcgaccatc gacgccatgg
     1681 ctcccgcgac caggagcgtg acgccggtgt cgagcagggc cagcgcgacg agggtcagca
     1741 cgatcgcccc caccgtccgc agcccggcca tcagtcgcag cagtcccggg tcggaggccg
     1801 cgaagcggtt ccaggtgccc gtaacggtcc gtcgtattgc tgccttcacg ctgccgtacg
     1861 ctccccatgt ttggcatcgg ccgaggtcac gtgcctcggc cgggcgcccc cagcatcgca
     1921 cgagggccgt cggaacggcc gtcgtgtgtg ccgtcaggtg gcttcgtagg cggcacccgc
     1981 gaccgcgagg tcctcccagg ccatgccgac gctcttgaac aggcgggggc ggtcggggtc
     2041 gacggcggcg gctccggtgg ccaggccggc gagcgtcacc agtgcctccc ggtcgagccg
     2101 gccgctctcc agctccggga tgaggtcgcc ggcttcccgg gccgcggcgg accgggactc
     2161 caccacgacc gtgctgcgca ggacggtcgt gccgtccacc tcgcggctgc cgcgctcgtg
     2221 ggagccgacc gcggtcacca tggcgtgcgg cgggagcagg gcgccgtcga acagcggggc
     2281 gcgggcggtg gtgcagcagg cgacgaggtc ggtgccggcg accgcggacg gctcgccggc
     2341 ctcgacgcgc aggccggtgt cccggcgggc cgtcgcgacg aaccgctcga cgttggcggg
     2401 ggtccgcccc accacggtga catgggtcaa cggacgcacc gcgcgcagtg cgtccaggtg
     2461 gccgcgggcc tggggaccgg tgccgaacac caccaggtgg ctcgcctcgg ggacggcgag
     2521 caggtcggcg gcgagggcgg agacggcggc ggtgcgcacg gtggtcagcg cgatgccgtc
     2581 gagcagcgcg agcggggcga gggtgggggc gtcgaagagc aggtactggc cctgcacgcg
     2641 ggggagtccg gcggccgggt tgtcgggggc gacggtggcg atcttgacgc ccgcgtagcg
     2701 gctgcggtgc gcgggcatca ggagcagttg gccgtgctcg acgggcacga tggtgcgcgg
     2761 cgggtcggct tcggggtccg gcccgtcgag cagcgcggcg cgcagtgcgt cgaaggccgc
     2821 ggccatcggg agggcgcgca gcaggccggt ctcgtcgatg gtccggggca ggtgggggac
     2881 tgggggcgtg gcgctcatac cgccgagtct gccccggccg gctgccggga tgcgtgatct
     2941 tcgacgcacc ggacgtgcgg ggcggggagg ggaggagcgc ccgccggagc gctcctcccc
     3001 acggggtcag caggtgccca ggtcctgcca gacccccgag ccgccggtgg acggcgcctc
     3061 gccctgggtc caccaccggg ccttccaggt gtggccgttg taggagacgg tgtcgccgcc
     3121 gacgtagacg gtgcctgtgg cgtaggggct ggcggcgcag ctgccgcccg ggggcgtggt
     3181 gggcggcgtg gtcgggggcg tggtgggcgg tgtggcgccg gcgaacttcg ccgagtactt
     3241 ggtgaagtcc caggcaccct gcggaacgct gctgcaggtg ccggaggtcc ggccgttgtt
     3301 gtccggcggg gtgcactgtc ggtcgcggtt gacggaccag ttggtgaagc gcgccatgtg
     3361 gtgggaggtg gcgtagtcca acacggtctg gaagtccgcc tgggtgaaga actccccggt
     3421 gtcgctgcgg ccgttcatct gggagatgcc ctcgtgggcg taggcggtgg cctcgtccca
     3481 gccgaaggtg gagcgcagga cggcgttgaa gttggtcagc gcggaggtct gggcggcggc
     3541 gccgttgaag ccgccgtcga acggcatgat ggagaagttg tccggcacga aggactggga
     3601 cttggcctcg ttcagcatct gcttgccgaa ccaaccggtg ccgtcggccg tcccggcggt
     3661 ggtgacggag agatagaggc cggggttctg ctgctggagg atcttggcgg cgccgatctc
     3721 gttgtggatg gccgcggagt tctcgtactc cggttcctcc aggtcgaagt cgagtgcctt
     3781 caggccgtac ttgtcgagga cctgctggta ggcggcggcg gtcgcctcgg gcgtgccgca
     3841 cgcctgcccc agcttggtgc cgttgtagcc gccgatggac gcggagacgt cgccgccctt
     3901 ggcgcggatc gcggagatca ccccggccac cgcggtgtcc gacgccaccg ggtggagacc
     3961 gtcccaggtc ggcgagcagc cgccgccgtt gggcgccagg acgaaggcga gctggaacgc
     4021 cttgaggccg gtggcgtcca tcaccgcgcc ggcgtccggc gggtcgttgt cgagcggcat
     4081 gaggtacggc gccgccgcgt accagttgcc gcccagcgcc ttcggtgccg gtgtcgcggc
     4141 ggcgggccgc ggggcggcgc gctggccggt ggcgagcgcc gtgccggcga caccgagcgc
     4201 gatacaggcc gccgagacgg cggcggccag ggttctgacg tgacgaggtg gcacttgacc
     4261 tccacgagcc gacgaggggg tggtccagac caatcgacca cagcgtgaac cttcgccgtg
     4321 ggcacgtcaa cactttggac cggaccaagt cggcgcggcg ggcgggccgc cgaggcggcg
     4381 aacacttacc ggccgcacga tcacctagac tcgcgaaaat ggccaagtac ttcgacgtcc
     4441 acccggacaa cccccagcgc cgcaccatca ccacggtcgc cgacaccgtc cgctccggcg
     4501 ggctcatcgc ctacccgacc gactcctgct tcgccctggg gtgccgactg ggcagccgcg
     4561 acggcatcga ccgcatccgc tcgatccgcg ccctcgacga ccgtcaccac ttcaccctgg
     4621 tctgccagaa cttctcgcag ctgggccagt tcgtacagat cgacaacgac gtgttccgcg
     4681 cgatcaaggc ggcgaccccc ggtcgctaca ccttcatcct cccggcgacg aaggaggtcc
     4741 cgcgccagct cctgcacccg aagaagaaga ccgtcggcgt ccggatcccc gaccacaccg
     4801 tcacccaggc actcctggag gagctgggcg agccgctgct ctccagcacc ctgctgctgc
     4861 ctgacgagga cgagccgctg acccagggct gggagatcaa ggagcgcctc gaccacgtgg
     4921 tggacgcggt ggtcgactcc ggcgactgcg gcaccgaacc caccaccgtc gtcgacttct
     4981 cgggcggcgt cgtggagatc gtgcggcggg gcgccgggga cgcgtcgcgg ttcgagtgag
     5041 cggcggcgga gcgatggcag caaaacggaa ccgcgcgata ccggcgctcg cggtggcgct
     5101 ggcgctccag gcgggcgtgg ccggggtggc cacggcccag gcggccccgg cggtggcgag
     5161 cgcccccgcc gcggccccgg ccgccgcgat caggatcacc gaggggcagg tcggcgaggt
     5221 cgggcccggc cgcacgatcg tctaccccgc ggtcaccagc tgtctgacgg tcaccgtccg
     5281 gctggcggac ggtgggaagg tcggcgcgca cgccagcctc ttccaggtgc ccggggaata
     5341 ccggtccgac cggatcctcg ccgcggtcca gcagcggatc gggacgcggg cggtacgcgc
     5401 cgtggaagtg cggggcgcgg tcggtgcctg gcatcccagc tacttcacca gggcgatcga
     5461 gagctacccg gagggcgcac agatcccggt gcccaccgcg cccgaccccg acggcctggc
     5521 ccgggcggtg gcggacggcc tcggccagcc gcgcggcaag gtcaccgtgc aggacctccc
     5581 cgacggggac cagacggtgg agtgaatcgg agcccggagc ccggagcccg gagcccggag
     5641 cccggagccc ggagcccgga gcccggagcc cggagttcgg agcccggagc ccggagttcg
     5701 gagtctggag tctggagtct ggcggtcgga cagtggaggc cgggcgcccg gggtcggaca
     5761 cttcttgaac gcggcggagg caagtccccc gtaccgcacg gaagcgcgta gcggcaccaa
     5821 ctgcctctat cagcacatgt gttgacggtc ggtcggtgtc gtctcgtgaa actcgggtcg
     5881 tggctcttga cgcacccttc acgaagctga agcatgcgac ggcgggagta cgctccccac
     5941 cccccacatc ttcacttcct gaatcaggag agtcacccat gacccctgcc tccaaggaac
     6001 cccccacgcc cgaacgtccc accgtcggcc gccgcaccgt cctcggcgcc gccgccgcgg
     6061 tggcctcggc ccccgccctg ctcggccctg cccggaccgc ccacgccacg ccgccgcaca
     6121 gaaccccggg tgccctgccc ggcggcggcg atctcggccc caacgtcctc gtcttcgacc
     6181 cggccacccc cggcatccag gcgaagctgg acgaggtgtt caagcagcag gagaaggccc
     6241 agttcgacac cgggcgctac gccctgctgt tcaagcccgg cacctaccac ggcctcaacg
     6301 cccaactcgg cttctacacc tccatagccg gcctgggact gtcacccgac gacaccacca
     6361 tcaacggcga cgtcaccgtc gacgccggct ggttcaacgg caatgccacc cagaacttct
     6421 ggcgctccgc cgagaacctc gccctcgtcc cggtcagcgg caccaaccgc tgggccgtcg
     6481 cccaggccgc gccgttccgc cggatgcacg tccgcggcgg cctcaacctg gcgcccgccg
     6541 gggacggttg ggccagcggc ggctacctcg ccgacagccg catcgacggg accgtggagc
     6601 cgcgttccca acagcagtgg tacgcccgcg acagcgcggt cggcggttgg tccaacggcg
     6661 tgtggaacat ggtgttctcg ggtgtgcagg gcgccccggg gcagacgttc cccaacccgc
     6721 cctacaccgt gctcgacacc acccccctct cccgcgagaa gcccttcctc cacctcgacg
     6781 gcgccgacta ccgggtcttc ctgccggagc tgcgcaccaa cgccagcggt gtcacctggg
     6841 gcaacggcac cccgcgcggt acctcgctgc ccctgtcgca gttctacgtc gccaagcccg
     6901 gcgtctccgc cgccaccctc aaccaggcac tcgcgcaggg actccacctg ttgctcaccc
     6961 cgggcatcta ccacctcgac cagccgatcc aggtgaccgc gcccaacacc gtcgtcctgg
     7021 gcctcggcta cgccacactc atccctgaca acggtgtcac cgccgtacgg accgccgacg
     7081 tcgacggcat ccgcctggcc ggcttcctca tcgacgccgg cccggtgaac tccgccaccc
     7141 tcctcgaaat cggcccggcc ggcgccgccg cggaccactc cgccaacccg accaccgtcc
     7201 aggacgtgtt cctccgcatc ggcggcgcgg gcccgggcaa ggccaccacc agcatcgtcg
     7261 tcaacagccg gcacaccatc atcgaccaca cctgggtctg gcgcgcagac cacggcgacg
     7321 gcgtcggctg ggacaccaac cgtgccgact acggcatccg tgtcaacggc gacgacgtcc
     7381 tggccaccgg cctgttcgtg gagcacttca acaagtacga cgtgcagtgg tacggacagc
     7441 ggggccgcac cgtcttcctc cagaacgaga aggcgtacga cgcccccgac caggcagccg
     7501 tccaggatgg ggacgtcaag ggatacgccg cctacaaggt cgccgactcg gtcacctccc
     7561 acgaggcgtg ggggctcggc agctactgct actacaacgt cgatcccagc atcgtccagc
     7621 accacggctt cgccgcgccg aacgccccgg gggtgaggtt ccacgacctg ctggtcgtct
     7681 cgctcggcgg caagggccag ttcgaacgcg tcatcaacga caccggcgcg ccgacctcgg
     7741 gcagctcgac ggttccctcg accgtggtgg cgtacccctg acctccggcc cgtcccgtac
     7801 caccgcccgg cccgccgtgc catgggagga ggccatggca cggtcggtcg gcttcggccg
     7861 cggcgccgaa ctcctcgtcc gccactggcg acgggcggcg ctcagcccat ccggtagtcg
     7921 aaggtgtacg ggaaggacgg attgccggcc tcgtacctga aactgcccga cccgctcagg
     7981 ccgaccaggt ccccggtggc cgatccgggg acgacctcga aggtgcaacg caccgtgccg
     8041 tcggcgtcga aggcgccgcg ctcctccagc acgaaactgc cctcccgccc gtccagccgc
     8101 ccggtcagca gctccagacc ggtgaacgtc cccgtcttgt ccgaggtgta gacgatggtg
     8161 tactcgcagg ccgtgcggtc ggcctcgatg ccgccggaga agttgttggt gacacaggcg
     8221 cgcgcgagcc gcgggctcgt cgcgtccgaa ccgatcggct gctcggccca gttggcgtag
     8281 ctgatgcggc cggtggtctg tgcgggcatg gctgtcctct cggtctcccg tacggccggt
     8341 gccgggcggt gtgcagccat cctgaccgcc gtacctgaca ccttctgtca ggtacgcgag
     8401 acaatcggag catgcgcgcc gaccggctcc tcgccctgct cctgctgctc cagaaccgcg
     8461 ggcggatgac cgccgccgaa ctcgccgcgg aactggaggt gtcggtccgc acgatctacc
     8521 gcgacatcga ggcgctcggc gcgtccggcg tcccggtccg caccgagcgc gggccggacg
     8581 gcggctaccg cctgatggac gggtaccgca cccgactgac cgggctcacc ggtgccgagg
     8641 ctgatgcact cttcttcgcc ggagcacccg gcccggcccg cgacctgggg ctgggtgcgg
     8701 tgctggcgac cgcccaactg aaggtgcagg cggcgctccc ggtcgaactc gccgagcgga
     8761 cccggcggat ccaggaccgc ttccacctcg acgcaccggc gtggttccgg gacgccgatc
     8821 cggtcgccca cctcgcgccg gtcgcccggg cggtgtggga gcagcgggtg gtccgcaccc
     8881 actaccggtg ctggcgcggc gaggtccacc gcgagctgcg cccgctcggc atcgtcctca
     8941 agggcggcat ctggtacgtg gtggcgctcg ccgacgaggc ggtgcggacg taccggatcg
     9001 cacgtttcca ggccgtggag ctcacggacg aggcgttcgc ccggccggcc ggcttcgacc
     9061 tggccgcgta ttgggccgag tcctcccgcc gcctggccgc cgcgctgcac caggacacgg
     9121 cgcgcctgcg catctccccg caggcccggc gcctgctgcc gatgctctac ggcgcggtgg
     9181 gccggcgggc gttggagggc gccggggagc cggccgcgga cggctggacg gaggtgacga
     9241 tgccggtcga ggggccggcc gtggccgtcg gcgacctgct ccggctgggc gcggaggcgg
     9301 aggtcctcgg cccacccgcg ctgcgccgcg ccgtggcgga ggcggtgacc gtcctggccg
     9361 gccgctacgc gacggcggac gacccgtcgg agtgacccgc ttcggccagg accgagctgg
     9421 tccgcacggg agttcagccc tgcggactga gctggatctt gcggccggtg ccctggcgga
     9481 aggtgtggag cgtggtgtcg aagccggggt tcctggtgcg ccccgctccg gctccgcggg
     9541 gcggccgggc cgcccgactc cgtaaaatca acaaaatctt cgatccttct ccatatccgt
     9601 caacgccgcc tatctatacg gcatttggca tggacatccg ccacatcggg accaatcacc
     9661 ccatttgcgc ggtgcttttc gatgctccgt gccacgaact tcccctgaaa cataggttgg
     9721 ttgtttttcc acgtgatatg agccacttga caccccaacc gccccggcat tcacttctgc
     9781 ggcggtcgaa gaccctttcg gcggcctgcc acggggcaca gtcagcccct gccaaggagg
     9841 tacggatgaa gttcgttcgt ctcgtgaagc ggatgcgcac cgaaaagagc ctcaaggcgt
     9901 acgcctggta cggctggatc tgatcggccc acgcccgcaa cggacggtgg cgggccgcac
     9961 gcaagggtcc gccaccggcc cgcgttcccg gaggttcccg atgtcccagc gaccgaccgt
    10021 tacccgcggc gaccggcggg tgcgcttcgc gcccaggatc gacgccctgc tgcaccgcca
    10081 caccggtcag gagctgttcc gactggagcc caccacgatc ggcgtcgcgg gcgccgacct
    10141 gatggacgcc ctgctgcgca gccggccggc caacgccgag gagcgcccca ccttcaagcc
    10201 cgtcctcggc cggcacgtcg gccgggccga cgccgccgcc tacatgcagg ccctgggcgc
    10261 ggacgtgcgg aaggccctgc agcgccccct ggagaccttc gtcgatctga ccggcgtctg
    10321 gccggcggtg ccgcacgcct acctccgcga cctcgtcttc ggacaggaac tcggccgcct
    10381 gcggatcctg gtcgaccgga gactggaact caccccgaag ctgacctggt ccgccgtcgc
    10441 gtcgggcgcc gccctgctgc gccgccccga cccggcggag ccgctgtcga agctcgccac
    10501 cctcgccctc ggcgccgccg gctacgccga ccgccgctac gccctgtacc tctaccgccg
    10561 ggtggcggca cccatctgct tcaccgtggc cgcgttggtc accaacgcgg tctggctggg
    10621 cgccccgttc gacgacgccg tcccgaaccg gcaactgctc gccgaggcgc tccggctgtt
    10681 gcccccgtcg tggaacatcc tgcggatggc ctcgcccgaa ctcaccgccg tggacgaccg
    10741 gatcggcccg accgacgacg tcctcctgct gcccctgctg agccatcgcg acccggcgtt
    10801 gtgggaggac cccggtgcct tccggcccga gcgctgggcg gatctggacc cggacaacca
    10861 ccccggatac ctgccgttcg gccacgccga cgagcgctgc tgggcccgcc acatggtgct
    10921 cccgctcgcc gaacgcatcc tggacctggt gcgccgggac ggcctcacgg tgcgctccgc
    10981 ccagaccgtc ggccgggtcg aactcgacgg tctgatggag gtcgcggagg tccgggtgac
    11041 ccgaggctga accccgtccc gccgccacga gccagcggag ggcgggcctg acacgacatc
    11101 acagtcatgt cagcgactgg acgttgtccc gcacatacgc ggcgaaggaa cggggtggcc
    11161 gcccggtgac gcgggtgacg gcatcggtca cccggtcctc ggcgccctcg gcgatccggc
    11221 ggtccaggtc cgcgaggatc gccgcgaagt ccgccggaag atgccgcgcg agccgcgtgc
    11281 ccagttccgc ctcggacacc gcccggtggc gtacgggtcg gccgctgacc tcggtgagga
    11341 tcgcggcgac ggcgtcgtag gagagggccg ccggcccggt gagcacccag tcggtgttgt
    11401 ggggcacggg gtcggtcagc gcgtgcgcgg ccaccgcggc gatgtcgtcg gcgtcgatga
    11461 acgcgacccg gccccgtccc gtcgccgtgg tcagcaggcc gtcagtgcgg atggcgtccg
    11521 cgtgcgggtg gtggccggga cggccagggg attcccgtag tcgcggtagt gggcgatgcg
    11581 gccgttggcg acggcgacca ccgcgacgta cgacatcgcg aacggctcgt cggtggcgac
    11641 caggcggccc acggcgctca tctcggcgat gatcgtgccg ggggcctggg tttgatggat
    11701 cgtcaggtgc gggacgtcgt gcagctcgat gtgatggggg tagtcgcgca tagaggcggc
    11761 gacggcggac ctgccctcca ggcggcgcgg gcggccttcg ggggcgaagg ggaactcgaa
    11821 gacgccctgg tcgtcccaga ggtccaccca tgcggcgaaa tcctggtcca gaaggaggcg
    11881 gggcccgtga cggaagaggt cctccggagt ccgatgggcg gtcgtggcgg gggcatctcg
    11941 cttctcctcg atcgggagcg tgcggcgcgg tcgttcgata ccgttggacc gccggtccgt
    12001 atcgtcgata cggaccggcg gtccgtatcg caagagaggt catgaccgat gcccgaaccc
    12061 cgacagcgcg cggacgccgt ccgcagtcgt gaagccgttc tggatgccgc cgaggcgctg
    12121 ttcgcgcgca gcggtcccgc cgaggtgagc atgaacgccg tcgcgtcggc cgccggcgtc
    12181 ggcaagggaa cggtcttccg ggccttcacc gaccgggcgg gactgctcca ggcgctcgcc
    12241 gaacgacgca ccgcggcgct ccgccgggcc gtcgccgaag gacccgcccc gctggggccc
    12301 ggcgccccgc cgcacgagcg gatccccgcg gtactcgacg ccctggcccg cttcaaactg
    12361 gacaaccaga gcctctacct cgtcctggag gaagccggcg cggccagccc gtaccggagc
    12421 ccgtcctaca cctggtggca cacgacgctg cgcgacagcc tggcgcagtg cgccgacgcc
    12481 gggcgcgccg acttcgtcgc ccatgccctg ctggccgccg tccgtgccga cctcatcgcg
    12541 cacctgctca ccgtggaggg gctgtcggtc gacgagatcc gcgaccgact ggccgtctac
    12601 gtccgctccg tgctgccgcg tgacacgcac gcgtgaggcg cccccggtcg gggtcgcggc
    12661 cgccggcccc tggcctggcc ggccttcacg tcacgtcacg gctggccgat caggcccgtc
    12721 cgcaaccggc ccaggatccg ggcgagcagc cgggagacat gcatctgtga caccccgagc
    12781 tcctcgccga tctccgactg cgtcttctcg tggacgaacc gcagggtgag gatccggcga
    12841 tcccgccgat cgagcttccg gaccaggggg gtcagcgcat ggaggtcctc ggcgagctgc
    12901 agccgcgggt cctcttcgcc gagggcgtcg gccaggctgc gggccgactc gtcctcggcc
    12961 gggtcgctgg gctggtccag cgaccccgcg gtgtagccgt tgccggcgat cagcccgtcg
    13021 atcacctcgt cctcgcccag gcataggtgc tcggcgagtt cggcgaccgt ggggtcccgg
    13081 tccaggcggg cgaagaggcg ctcctttgcc tgggcgatgt cgatccgcag ctcctgcaac
    13141 cgccgcggta cgtggaccgc ccaggtggcg tcccggaaga accgcttgat ctcgcccacg
    13201 atgtagggca ccgcgaacgt cgtgaactcg acctcgcggg acaggtcgaa gcggtcgatg
    13261 gccttgatca gcccgatggt gccgacctgg acgatgtcct ccatctcgct cggatcgccg
    13321 ccgcggttgc ggaaccgggc ggcggcgtac cggacgaggg agaggttcat ctcgacgagc
    13381 gtgttgcgca cgtactggta cgcgtgggtg ccctcctcca gcaccgcgag ccgctcgaag
    13441 aacggccggg acagtgcctt ggcgtccttc ggcgccatct tggacgcctc tgcgatgtac
    13501 ggcagtccgc cggttccggg actcacggtc cggtggccct gtacctggcg ctggccgtgc
    13561 cgcgttccgg tgtgtgtgga aactgccgtc gtcatggctg ccctcccata atttggatca
    13621 tggtccggta agccgcgtct gcccttcgtt cgcggaacaa aacatcagcc ccatcttttt
    13681 tgccatccag gatcttgacg ctgcgtcagc aggtgtggtg cggcgatgcg cactgctctc
    13741 ttccggccat cggacgcatc gtggccttcg tcccgcgctc ggttcggtct ccccgtggcc
    13801 cttccatcgg cccttccacg gcctttccgt ggtctctcct cggctcgccc agggtccctc
    13861 tcggcccggt cgggggcgtc agtgcgcagc cccgatgcgc cggtcgagct gttccaggac
    13921 gtgcggccag gcgtcccggt gacggtcgcg cgccgcctcg tcggcgaacc cggcatggct
    13981 gagccgcagc tgcgtcccgg agccctcggc ggcgaatgcg accgtgacca cggtctccgc
    14041 gccctgggtt ccgcccgcgc cggtgaccca cgtcagctcg accagccgat ccggatcgag
    14101 ccgcaggaag cggccgtagt gcgggtggcg ggccccctcg tgctctgtct cgaagaagaa
    14161 cgccgtgttc acccggccct ccatcagcac cgatccgggg gccgcgaacc agcggtcgaa
    14221 ctgctcggtc caggcccggt acagcgcggc cggcgttccg ctcatgagtc gttcgacggc
    14281 gagctggtgc ggccgggagg agagatctgg caggcgcatc ggttcattgg acatggaggg
    14341 aatgtagcgt aacggggcaa aagtggcggt tccttgctgg gtcgaagggg gcccgatcgg
    14401 cgtccttgcc cttcgctggc cgcgactctt ccgcaccgtt tcccggacct ccgcggattt
    14461 caccgggaac gggccgggtg gggtggggcg cgggtgggga aaactggggc cgacgtgggc
    14521 cggaacgtgg atttctacgg ggagttggcg ttgacggggg acggtgcgcc gactgtcgag
    14581 atgagcgcac tttggccttt ccggcggatg gcaagtcgcc cggaccgctc ggttagggtc
    14641 ggacgcatga gtgaggagat cgccgcactg gtacgtcgtc tggcatccga gagccgggag
    14701 gatcgcgcgg ccgcacagga agcactgacc gctgtcgggg ccccggtcgt caaacacctg
    14761 ctgccgctgc tgcgcaatga cgaaacgccg ggcagctcgg ccgcggaatc ctgtctggaa
    14821 agcctggggg acgcggcaat cgatccgctc cgcgaggtgc gcgcggaggg tcccggaagc
    14881 ctgcggaagg ccgcgctgcg catgctggcc acgatcggcg gcgccgatgc cctcagtgac
    14941 gcggatcggg cggccgtgga gcggttggtg cagatcaaac tgccggacga ggagccgggt
    15001 gatctgcctg ccgactgctg gatcgcggtg cccgaggcgg aaatcggcga tatcgtccgg
    15061 gcgttggacc tgcacgacgt ccgcccggtc acgacggcca tgggtctgtc cgccgccgtc
    15121 gagcacgaga attccctccg gcgccccacg gaagacggcg gcgtcaccac ggcataccgg
    15181 gtcttcatca ccccggaatt cgacggctgg cgactgatct acggtgtcga ttacctcaac
    15241 gacaactggg cgcagatcgt gacacgactc agtgcgcgct gccgggaagc ccacttctac
    15301 gccatcgacg actacaacgg cgcgcaagtg tggtgggttt cggagaacgg acacgacaag
    15361 cgcggctacc gcacctacgg cgacccggag tggatcgggg aaccgatgga attcgagcgg
    15421 aacttgatgc cgggcggcga cgcgtacgac gacgaggacg acgacgccga gcagtacgcc
    15481 gagggcgtcc gtgatccgga ggaggtcgct tcctcgatct ccatccagcc gaccgtggtc
    15541 gaggtgctcg accgggtcgg gcacggctgg ctcgcggtga ccgacccggt cgtcgggcac
    15601 ggccggttcc ggggcgccct ggacatctga gggcgccccg aggcggtgcc gccgccgtgg
    15661 cagaaggccg gcggcggcac ccgcggagtg cgtcggcgct gtccgctaca gcagactcgg
    15721 ctgaatcttc ttccaggtcg agtccagatg ggcgatatgc cggtccattt cgcggaccat
    15781 cgcctgcttt tcgggcgtcg tcatcccgtt cttacgcttg gtgaactccg cgtcgatcat
    15841 gtccgagagc ttcttgcgct gttccgcttc gctgagcttg tcgatcttgt ccttctctgc
    15901 cgcgatccaa ttcgcgctct tgccctcgcg gcgatcgagc cggtcgttga tctccttctg
    15961 cacgccggcg atgggcttct tgtcgaactc cttcttcagc ttcttcttct ccgtgtcgag
    16021 ctgcttcttc gccttgacgt cttccgggtc ggttcggtag gtcgtgctgt agtacacctt
    16081 cgtgtcttta ggagtcattt ccctcagcac gttcgaacac ttcgcgtgcc cctggccgtt
    16141 gccgcacggt tcacgctcgg tgtagagggc cttcgcgctg tattgcgcgc cctcttgatt
    16201 ggcccgatta agccactcca tgagatgttt ctccgagtgg cgtcccgaca cgtacttctc
    16261 gcccaccgga accgaggaat cggcaacgta gtgggtttcc cgcgcgggcg gcggcccttc
    16321 gacctccaac accgcgtagt tgcggtctgt gaagtccgga ctgttctgct tcaggctctc
    16381 cggcatcgga ccgtcgtgct tcagcttgtc gaggtacttg ttgccactgt tgcggtgccc
    16441 tcggacaccc agtgactcgt cggagttctt ggccgcactg tccgtggtgc cgtccttggc
    16501 atccggctcg ttcggaggac ggtacggctt gtcgacgtcc accggattgc tgccgacacc
    16561 gtgctgctcg tggaatccca cactgagccg ggtcagggac gagacgacgg cgagtttctc
    16621 accacggtcc aagtccttga atccgggcag ccgcttctcc aggtcgctct gggtgaactt
    16681 ctgcgggccc ttgcccgcgt cggggccgtt ggtctgcgga tggccggaag aggcccggag
    16741 cacccgtgcc aaacttccgt cgtccgccca gccggtcagc cggtcgtgga cccggtcgag
    16801 tgggatgcgg tgcacgtccc gatcgttgcg gagctgcgcc tggagcttgt ccggaacctt
    16861 cccgtactcc ttggaatcct tcttctccgc gtcgagagtg tgcttcccct ccttctcctg
    16921 cttggaggtg tcgctcgtgc cgcgatcacg cggatcggag tagggctctt tgccgtccgt
    16981 tttgccgtcc gttttgccgt ctgtcttgcc gtctgtcttg ccgtcggtct tgttgtcggt
    17041 cttgttgtcg cccttcccgc cgtccgtgtt gttcccgttg tccttcttcg ggtccgccgt
    17101 ggagccggtc gactgcccgt gatcctgctc ggaggcgttg ccgccgccgc tgttcgacgg
    17161 cccggcgggc ggctggccgt cgagatccat cggggtatcg ggagtgggct tgccgtacgg
    17221 cggcccggag gtgggatgct ccgccgtgcc ggagccggtg ttcgtccctt gggtctgcgg
    17281 tgcctgctgt ggcggtgaat gctgttgcgt gtggtcgtcg tcgacgtcca tgcggtggtc
    17341 gtcgaggccc agggcgtcca tgtggtccgg gagggaaccg agggtcgcct ggggcggtga
    17401 cggggtgtcg gtgtggggat cgataccaga ctgctggccc ggctgtccct ccccgcccac
    17461 ctttattcgg gtccgccggt gggaatcgga cccggactcg gagggcgagc tgacggactc
    17521 accgtcgggt gaaggctgtt cgctggcctt gcgcttgagg gcggcggggt cggccgccga
    17581 aggccgttcg tccttcccgt ggtccgggat cggtgcgcca cgcctcgcgg tgccgtcgcc
    17641 cgagtcgtgg gaaccgttcg ccgcaggggg cgactggtcc gcgtgcgcag tgtggtcgga
    17701 gtccgtgccg tcggacttgt ggtgcgaatc ggtggcaccg gaaccgttgg aggcatccgg
    17761 ggcgggatgc agcggcttcc ggtcggcgtc cagcgggatg tggaagacgt cagtggcgcc
    17821 ctcggtgttg atggggtggt ggctgacctc accgctctgg gtgtcgaccc agacgatctt
    17881 cccgttgtgg ttgacggcgt tgaacgcatg accgccgccg tccgcccagt tgacctggac
    17941 gatcgccgcg gagcccggcc ctgccttcag caactcctgg gcgatggcat ggtgcccgtc
    18001 cgggctggtg ccggcgtagg agtgcggcgc cccggcccag tcgatgatgt tctcgttggc
    18061 cttggactcc ggcgaccagt ggtccggggt cccgtccggg gtctggtcgg gggtccgggg
    18121 cgcggagacc tgggggttgc cgtaccaggt ctccaggaac gaacgactgc agtcggcgca
    18181 gttgttggaa cggcccggca cgttggtgcc cccgtcgttc tgcaactggg cccagccggc
    18241 cgtcggatcg gggaagcgct gtgggctgcc gtcgtcattg cgcgggacgg cgttctccag
    18301 cgcctgctgg tccacggtgt cgggcggcag caggccgccg ggggactcct ggataccgga
    18361 ccggatctcg tcgaggctgg gacggtcgtc cggtgtggcg gtggcgtcgg atgcgtcagg
    18421 tgcatctgac gtgtcagaca cgtcagacac gtcagacacg tcatctgggt cagatgggtt
    18481 gggcgtgtgg gaagtgtcgt ccttgttgtc cggcgcttgg tcgtccttgg ggcgcgactc
    18541 gctgtccggc ttggggtcgt ggttggtgtt cggcgactgc gagtcgtggt caggggtggt
    18601 gggcgacggc ggtgcgtcgc tggtgtgggg cggttgctgc gcgggccggg acggacgcgg
    18661 ggacggctgc tcgccgtgcg gacgctcggt gcgcggcggt tgggcggtgc gcggcggttg
    18721 ggcggtgcta cgcgcaggcg tgggccgtgg gcgcgacgac tggtcggccg cggtggggtt
    18781 ccgcgggtcc cgggtcggcg tgccgcgcgg gggtgcgccg ccgttgccgg cgggggagga
    18841 cggggcggtc gaccgcgggg gtgtcgtagc ggacggggat ccgcccacgg gagtcccggc
    18901 gggagcgccc gcaggcatgg tgccgccgcc catgacgacg ccggactgcg gcgattgctg
    18961 tgtagacggc ggcggggcgg cggccgagcc gggggtctcg ccgcctgcat gcgacggctg
    19021 ggcgctgccc ggcggcggag gtgccagggt cgcgctctcc gtgtgcacgg tgaccgggcc
    19081 gtcgtcctgg ggccggttgg gatcgggctg gtgcggcgca ccggacgagg agggaccgga
    19141 gtacccgtgt ccggacgggg ccgcatgggg agcgccgccg cccgatgcca ctccaccgcc
    19201 cgtcggcatg ctgggacggc tgcccccgcc gttgctgctg ctgttgccgt cgtcgtgctg
    19261 ggtgccggag tggtccggcg tcggggacgg ggagggggaa ggggacgggg agggagatgg
    19321 gtcagggact ggtgatggcg cgtgctgcgg ttgcgggccc gtatgggtac tggtgtcagt
    19381 gctgccgtcg ttcccactgt tggtgacgct gccaccagtg gtgccaacgc cgccagtgtt
    19441 gccactgccg ccgttggggt cgccgatccc gttgctgtga tgttcgggag tgggggcagc
    19501 ggagtccggg cgtggggcgg tgttggtgtt ggtgctgctg tcggtgttgc tgtcgttgtt
    19561 gctggtgtcg tgggaggtgg gtgtatcggt gttcggtcgg gcggacgaat ccgaggacga
    19621 cggggaagag gtcgcggacg acgaggcgtc aggcgtgggc gatgccgcct gtgcttcccg
    19681 gtaacccgcg tcgaacggcg tcagttggct cggcggcggg gcggcggaat gcgccggccg
    19741 gggcgagtta ccgccgtccg gcgagtgacc gctacctccg gccgaggagg aacttccgcc
    19801 gtcggcagct gagctcccgc catcgggaga ggagttcggc ggcgtcgagg agttgttcgt
    19861 gggggtggaa tggcctgccg acgacgatcc gcccgatgcg gtggccccac cacccgccga
    19921 cgatgcgccg gctgacgttc cgcccgcgga cgatccaccc gccggtgccc catccgtgga
    19981 cgatgagccg tcgcctcccg acggcgatcc gccgcctccg ggcgatgaac taccgccatc
    20041 cgagggcgag ttgctgccgc tcgacggcga gtggttgccc ccgggcgacg actggccgcc
    20101 gcccgaagtg tcggatcctg aggaggtgtt tccgcccccg ctcccgccgc ctgcggactc
    20161 atgtcccgaa ccgccgcccg agtcgccgcc ggagttgtgg ccggcggctg attccagacc
    20221 gttgcggacg tggtggcccg ccttggcgcc cgcgccgtcg cgcaacgcct cggtgaacgt
    20281 ctgccccgag ttcttgagcg cgttgacgcc ttcctcggcg cccttgttga cggtccggcc
    20341 gacgtcgaat ccgttctgcg cgccgaagct catgttcacg gactgcgcga tgacgtccga
    20401 gatcatggcc tggagcgccg agaccgcggg ctccttggcg gtttccagcg ccgcgtcgag
    20461 gacctgcttc gcggcctcct tgatgatttt ccggacgatc gcccgggtcg ccgcggttgc
    20521 cgccgcaacg ccgatctccg acaggccgaa cgtgaacggc gccgccgcct gggccgcgat
    20581 gatctccgcc gcaaggatcg ccaattgggc gatcaccgcg actttcatgc cgatgacgag
    20641 gccggcggcg acgtccaggg cgacggcgat ggtctccgcg gcggtccggg cgtcggcgaa
    20701 gtacccggag ccggaacctc cgttgaactt ctcccaggtg gtgttgaagc cgtcgatcga
    20761 gtcgccggag ttctgggaca ccacattgcc ggcagcggtg acgccgcgct gctggagctc
    20821 ttccacgccg gatgcgaagt tccgccactg ctgggcggat tcctgtaatt tgtcttcgtc
    20881 tgccgtgggc cagttgaacc ccagcatttc gaggacccac tcaagtgcgt ccggcagcat
    20941 taacgacaca gtggggatct ccaggcaggc gcgaggtcag gaagagaggg cagagcggaa
    21001 agggctgtgg agggggacgg gcagggcggc gcgagcaggg gcaccgcgag
//
