LOCUS       NZ_CP054019            10936 bp    DNA     linear   CON 28-JAN-2024
DEFINITION  Streptomyces sp. A1-5 chromosome.
ACCESSION   NZ_CP054019
VERSION     NZ_CP054019.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. A1-5
  ORGANISM  Streptomyces sp. A1-5
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 9459690)
  AUTHORS   Dashti,Y., Sumang,F.A.M., de los Santos,E.L.C., Alcantara,E.P. and
            Challis,G.L.
  TITLE     Structural revision and biosynthesis of polyketides FXJ15321 and
            FXJ15322 from Streptomyces sp. A1-5
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9459690)
  AUTHORS   de los Santos,E.L.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2020) Warwick Integrative Synthetic Biology
            Centre, University of Warwick, Gibbet Hill Road, Coventry CV4 7AL,
            United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: JUN-2019
            Assembly Method                   :: Flye v. 2.4.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Oxford Nanopore MiniION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/28/2024 04:28:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,328
            CDSs (total)                      :: 8,230
            Genes (coding)                    :: 7,847
            CDSs (with protein)               :: 7,847
            Genes (RNA)                       :: 98
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 156 of 383
            Pseudo Genes (incomplete)         :: 280 of 383
            Pseudo Genes (internal stop)      :: 26 of 383
            Pseudo Genes (multiple problems)  :: 73 of 383
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 22:08:01
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 8302897
            Orig. end                         :: 8313833
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP054019.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Philippine National
            Collection of Microorganisms.
FEATURES             Location/Qualifiers
     region          1..10936
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="34"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10936
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10936
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5936](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5936)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            complement(126..1376)
                     /locus_tag="HRD51_RS36655"
                     /old_locus_tag="HRD51_36185"
     CDS             complement(126..1376)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018537760.1"
                     /locus_tag="HRD51_RS36655"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HRD51_36185"
                     /product="cytochrome P450"
                     /protein_id="WP_205371206.1"
                     /sec_met_domain="p450 (E-value: 9.2e-23, bitscore: 79.6,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MESAEFSRNPYPLLAALRAGGPVQRVRTGKDRTTWVVTGWAEARA
                     ALADRRLSKDTARYFADRPSGRDLAPAVSRSMLATDPPDHGRLRKLAMAAFTPAAVSRL
                     EPRIREIAEGLAEALAGAAQAAGGGPVDLVEGFAAPLPITVICELLAVPDPDRAAVRRW
                     SDDLFAAADPDTADRASHALAGYLAELIAARRAAPGDDVLSGLIAARDAGDRLSEPELV
                     SLAVLLVVAGHETTANLIGNGLLALLRDDALRARLRDDAGLLPAAVEEFLRFDAPVTLA
                     TFRYATEPFDLGGARIGAGDVVMVSPGGANRDPARFDEPDAVRLDRPAPGGHVSFGHGP
                     HHCLGAPLARAEARIAFAVLLARFPALRPATDGRDASAGPDGVSWRRTRLMRGPARLPA
                     LLGPIAGGTNGAPPALR"
     gene            1560..1787
                     /locus_tag="HRD51_RS36660"
                     /old_locus_tag="HRD51_36190"
     CDS             1560..1787
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018092057.1"
                     /locus_tag="HRD51_RS36660"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HRD51_36190"
                     /product="hypothetical protein"
                     /protein_id="WP_039629879.1"
                     /transl_table=11
                     /translation="MSEHPPVIVHPPTPSGGRRVTVHGQIVGLAHGRGDVAEFLRRAGV
                     AGPAEDIALDDPRLVEWRGGSLDDWPMPSP"
     gene            1971..3449
                     /locus_tag="HRD51_RS36665"
                     /old_locus_tag="HRD51_36195"
     CDS             1971..3449
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018537758.1"
                     /locus_tag="HRD51_RS36665"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HRD51_36195"
                     /product="DNA-3-methyladenine glycosylase 2 family protein"
                     /protein_id="WP_235095262.1"
                     /transl_table=11
                     /translation="MSEDSRYEAVRSRDARFDGAFFFAVSTTGIYCRPSCPATTPRRRN
                     VTFFPTAAAAQGSGFRACRRCRPDAVPGSVEWNARADVVGRAMRLIGDGVVDREGVAGL
                     ADRLGYSARQVQRQLTAELGAGPAALARAQRAHTARVLLQTTGLPIAEIAFAAGFASVR
                     QFNDTIKEIYALTPSGLRAVRPGPGSRFGGLAAPSGTPGVLPLRLAYRGPYAARQIFDY
                     LAQRALGGIEEVTGEPGGRTYRRTLRLPHGTGIAEVDEATGDGWLDCRLHLTELRDLAT
                     AAQRVRRLFDLDADPFAVGERLGADPALAPLVARCPGLRSPGTVDPDELAVRAVLGRRA
                     GTAAGRALGTRLVAAHGELLPRPAGTLGHLFPRAVELADAPLTGLGLSDARIAALRALA
                     AALAARDVVLDAGTDRDRAERALRGLPGIGPWTADYIRMRALGDPDVLLGAPPAGTDPA
                     VWSPWRSYATHHLWAADPAGTAERHPNPTERIDA"
     gene            3446..3955
                     /locus_tag="HRD51_RS36670"
                     /old_locus_tag="HRD51_36200"
     CDS             3446..3955
                     /EC_number="2.1.1.63"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018537757.1"
                     /locus_tag="HRD51_RS36670"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HRD51_36200"
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="WP_235095263.1"
                     /transl_table=11
                     /translation="MTLYATLDSPLGELLLVGERSATAPGGTALASLSVPGQKRGAVVQ
                     DDWTHDPAAFAGIARQLRAYFDGAQQPFAVECVPRGTDFQQRVWAALEAIPYGTTTTYG
                     AIAAAIGASRGAVRAIGTAIGANPLLVVRPCHRVIGADGSLTGYAGGLARKQLLLGLEG
                     AAPAAR"
     gene            complement(4019..4783)
                     /locus_tag="HRD51_RS36675"
                     /old_locus_tag="HRD51_36205"
     CDS             complement(4019..4783)
                     /NRPS_PKS="Domain: PKS_KR (7-183). E-value: 5.2e-08. Score:
                     24.9. Matches aSDomain:
                     nrpspksdomains_HRD51_RS36675_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608009.1"
                     /locus_tag="HRD51_RS36675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HRD51_36205"
                     /product="SDR family oxidoreductase"
                     /protein_id="WP_205368417.1"
                     /transl_table=11
                     /translation="MGDLYGKTALVTGSSRGIGRGIAQRLAQDGAVVAVHYGSNEGAAK
                     ETVEGIRDNGGQAFLVGAELGVPGDAEALFDAFDAGLAAAGAEPGLDILVNNAAISLPG
                     HIGEVTTEEFDRTIAINTKAPFFIIQHGLRRMRDGGRIVNISSAVTSTAFPSTIAYGLS
                     KGAIDTLTRTLAKDVGERGITVNTVAPGFIETDMNAAMRSTPEAHAALAAISVFNRLGR
                     PADVADVVAFLASDDSRWITGQRIDVTGGSML"
     aSDomain        complement(4235..4762)
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_HRD51_RS36675_PKS_KR.1"
                     /evalue="5.20E-08"
                     /label="HRD51_RS36675_PKS_KR.1"
                     /locus_tag="HRD51_RS36675"
                     /protein_end="183"
                     /protein_start="7"
                     /score="24.9"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="TALVTGSSRGIGRGIAQRLAQDGAVVAVHYGSNEGAAKETVEGIR
                     DNGGQAFLVGAELGVPGDAEALFDAFDAGLAAAGAEPGLDILVNNAAISLPGHIGEVTT
                     EEFDRTIAINTKAPFFIIQHGLRRMRDGGRIVNISSAVTSTAFPSTIAYGLSKGAIDTL
                     TRTLAKDVGERGI"
     CDS_motif       complement(4691..4756)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_HRD51_RS36675_0001"
                     /evalue="6.70E-04"
                     /label="PKSI-KR_m1"
                     /locus_tag="HRD51_RS36675"
                     /protein_end="31"
                     /protein_start="9"
                     /score="12.4"
                     /tool="antismash"
                     /translation="LVTGSSRGIGRGIAQRLAQDGA"
     gene            complement(5001..5936)
                     /locus_tag="HRD51_RS36680"
                     /old_locus_tag="HRD51_36210"
     CDS             complement(5001..5936)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018537755.1"
                     /locus_tag="HRD51_RS36680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HRD51_36210"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_205371202.1"
                     /sec_met_domain="AfsA (E-value: 1e-19, bitscore: 69.3,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQQVQNPPAASRRGSDASAPIPHEFLHRPLVEDILIISWLRLSQD
                     RFSVTAQWPHDHLYFAPHDNRPHPMLAGETIRQAGLLLCHTEFGVPLGHHFILHDLVYT
                     ADPAHRVSGNGPTQLSIDVVCSQTRMRAGMLSSSRFDMTIRKDGRVVATGHSHVSVTSP
                     AVYRRVRGERITARHVPGPLPTPVPPGLTGSATERDVLLSPDGQPGRWLLRIAPGHPTV
                     VNPANDHVPGLSLLDAAQQAARTLTGSSSFLPYAFGTEFHRYAEHGRPCLIEARRKVPT
                     TTGTTTVLVTGAQDGEPVFTSVLTAMDARS"
     misc_feature    complement(6052..6065)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.86"
                     /tool="antismash"
     gene            6163..6861
                     /locus_tag="HRD51_RS36685"
                     /old_locus_tag="HRD51_36215"
     CDS             6163..6861
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018092050.1"
                     /locus_tag="HRD51_RS36685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HRD51_36215"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_235095265.1"
                     /transl_table=11
                     /translation="MARQDRAIKTRRVILESAAAVFGEHGFDRATIAEILSRAGVTKGA
                     LYFHFPSKEDLALAVLHEQVLDIAVRPQPIKLQEFVDAGQILAYRLRHDPIQRGAARLA
                     IEPGANQLDRKQSMSAWTTFVEGLLNEARARGEVLESVNVRDTAELFVGAFAGIQMMSK
                     AFTDQNDVSHRLLVFFEHTLPTIAVPAVLAKLRLDPERGARLDAELRERAAEEAEHAAA
                     LEADLQNAAP"
     misc_feature    complement(7135..7150)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 16.26"
                     /tool="antismash"
     gene            7294..9678
                     /locus_tag="HRD51_RS36690"
                     /old_locus_tag="HRD51_36220"
     CDS             7294..9678
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1005: Drug
                     resistance transporter, EmrB/QacA"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018537753.1"
                     /locus_tag="HRD51_RS36690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HRD51_36220"
                     /product="MFS transporter"
                     /protein_id="WP_235095266.1"
                     /transl_table=11
                     /translation="MTQTMTDQLPAGAADSAAAPPLPPPAKDGHRRTHAGIVPVLAFAG
                     IVVAVMQTLLVPVIKDLPTLLGTAPGNATWVMTATLLAGAVATPIMGRLGDLYGKRRML
                     LTSLAVMVVGSLICGFTDQLIVMIVGRALQGFAMGAIPLGIGLMRDELPRERLGSAMAL
                     MSSSIGVGGGLALPAAALVAQHADWHTLFFAAAGLGVLSMLLTALVVPETSVRAPGRFD
                     VAGALGLSVGLVALLLPITKASEWGWGSPRTLGLFGAAALILVLWGVMELRIADPLVDL
                     RTTARREVLLTNLASITVGVAFYAISLVLPQLLQLPTSTGYGLGQSMVVAGLCVAPLGL
                     TMMFVAPLYARIAARRGPKVSLLLGMLVIGVGYGGGIGLMQAPWQTVVIAVVVGAGIGL
                     AYSSLPALIIGAVDPSETGAANGLNTLMRSIGTSVSSAVIGMVLARMSEPLGPITVPTM
                     AGFRVSFLIATAAVALGVLVAAFLPSQRKAAGARATPVTQSADEAGTAADVPADAAGRS
                     APAAGAADEPAAGFRGRVVDTAGGPVPGATVTLIDRHGQQAGVTTAASDGSYALAAPAA
                     GTYVLTGAAPAHAPQAVSAAHAGEGTVAGADLVLTPTGSRLAGTVLGGPEGTALTGARV
                     VITDAHGDVVTHTTTGADGRWTVAQLPDGPYTLVFSAPGHQPTAHAVQLGGGVDGAGGD
                     TRHQEVRLRPGGTVRGTVRGPDGHPLADAAVTLLEDGTVVGHAVTGPDGAYAFADLVGR
                     HYTLSAAGYPPHAAPISLAGGADARLDLELAQPGGAGQRER"
     gene            complement(9813..10517)
                     /locus_tag="HRD51_RS36695"
                     /old_locus_tag="HRD51_36225"
     CDS             complement(9813..10517)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1071: GntR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018537752.1"
                     /locus_tag="HRD51_RS36695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HRD51_36225"
                     /product="FCD domain-containing protein"
                     /protein_id="WP_205368414.1"
                     /transl_table=11
                     /translation="MPLEALRPSPLVEQAAQQLRDQITGGHWPVGSRLPGETTLAKTLG
                     VGRSTVREALRALAGAGMVQPRQGAGVFVIAAEPTEDWPTRLRRAAVADVYEVRAMVEV
                     QAARLAAERRTEADVTALYAALDRRRADGADDAAFVDADIALHAAVVDAAHNPVLTDLF
                     GQFVPALRQGLIDLVRLLGLRDHDPHHGDARHAALVEAVAAGDADTAGDVLQSELAETR
                     ALLLTGRPAEAG"
     misc_feature    10586..10599
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 18.78"
                     /tool="antismash"
     misc_feature    complement(10912..10925)
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: weak, score: 18.1"
                     /tool="antismash"
ORIGIN
        1 cggaccgcga cgggccatgg acgaccggcg ccgcgaccgc cgcactgcgg tcgcggcgcc
       61 ggtgccttga agagggtcgt cgcacgccga acggccgggc cgggccgtcc gcgagccgcc
      121 tcgcctcaac gcagcgccgg cggcgccccg ttcgtgccgc ccgcgatcgg gcccaggagc
      181 gccgggagcc gggccgggcc gcgcatgagc cgggtccgtc gccaactgac cccgtcgggg
      241 ccggcggagg cgtcccggcc gtccgtggcg ggtcgcagtg cggggaagcg ggcgagcagt
      301 accgcgaatg cgatccgggc ctcggcccgg gccagcggag cgcccaggca gtggtgcggt
      361 ccgtgcccga aggacacgtg gccgcccggg gccgggcggt ccagccgtac cgcgtccggc
      421 tcgtcgaacc gcgccgggtc gcggttggcg ccgccgggcg agaccatgac cacgtccccc
      481 gccccgatgc gggcgccgcc gaggtcgaac ggctcggtgg cgtaacggaa ggtcgccagc
      541 gtcaccggcg cgtcgaaccg caggaactcc tccaccgcgg cgggcagcag ccccgcgtcg
      601 tcccgcaggc gggcgcgcag cgcgtcgtcg cggagcaggg ccagcaggcc gttgccgatc
      661 agattggccg tggtctcgtg cccggcgacc accaacagca cggccagcga gacgagttcg
      721 ggctcgctga gtcggtcacc ggcgtcccgg gcggcgatca agccgctcag cacgtcgtcc
      781 ccgggcgccg cgcgccgcgc cgcgatcaac tcggccaggt acccggccag ggcgtgcgag
      841 gcccggtcgg cggtgtccgg atccgccgcc gcgaacaggt cgtcggacca ccggcgcacc
      901 gcggcccggt ccgggtccgg caccgccaac agctcgcaga tcaccgtgat cggcagcggc
      961 gccgcgaacc cctcgaccag atcgacgggg ccgccgcccg ccgcctgcgc ggctccggcc
     1021 agcgcctcgg ccagcccctc cgcgatctcc cggatgcggg gctccagccg gctcaccgcc
     1081 gcgggggtga aggccgccat ggccagtttc cgcagccggc cgtggtccgg cgggtcggtg
     1141 gcgagcatgc tccggctgac ggccggtgcc agatcgcgtc cggagggccg gtccgcgaag
     1201 taccgtgcgg tgtccttcga caaccgccgg tcggccagcg ccgcgcgggc ctccgcccac
     1261 ccggtcacca cccacgtcgt gcggtccttg ccggtccgca cccgctgcac cgggccgccc
     1321 gcccggagcg cggcgagcag cgggtagggg ttgcgggaga actcggccga ctccatgggg
     1381 ttgttcactg ccaccctcgt gctctcgttc gccgtcagat ccgcgcccgc cctgccggac
     1441 cggcccagtg accaccttcg caccggcgcc ggtcgccccg tccggtccgg atcccagccg
     1501 tccgggtgag atcccaccgc cgaggtgcga gcgcgcggag cccggcgcac gctggaggca
     1561 tgtccgagca tccgcctgtc atcgtgcacc caccgacgcc ctccggtgga cgccgggtga
     1621 ccgtgcatgg gcagatcgtc ggcctggccc atggccgcgg cgacgtggcg gagttcctcc
     1681 ggcgcgcggg tgtcgccggc cccgccgagg acatcgcact ggacgacccc cgcctggtcg
     1741 agtggcgcgg cggctccctg gacgactggc cgatgccgtc gccgtgacgg gccccggccc
     1801 ggcacttccc agacgcgccc ggcccgtcct cccccccgag cccacgccgg cccccctgct
     1861 cgcatccatg ggcacccgtt gtcgctcgac gtgttcctcc tggctgccga cagcgcggtg
     1921 tccgatttcc gccagagcgg tggcgcggaa tgacgcacag tgggggtgtg atgagcgagg
     1981 acagcaggta cgaggcggtg cggagccggg acgccaggtt cgacggggcg ttcttcttcg
     2041 cggtgtcgac gacggggatc tactgccggc cgagctgccc cgcgaccacc ccgcggcggc
     2101 ggaacgtcac cttcttcccg accgccgcgg ccgcgcaggg ctccggattc cgggcctgcc
     2161 ggcggtgccg cccggacgcg gtccccggct cggtcgagtg gaacgcccgg gcggatgtgg
     2221 tggggcgggc gatgcggctg atcggcgacg gggtggtcga ccgggagggc gtggccgggc
     2281 tggccgaccg gctcggctac agcgcccggc aggtgcagcg gcagctgacc gccgagctcg
     2341 gcgccgggcc cgcggccctg gcccgggcgc agcgcgcgca caccgctcgg gtgctgctgc
     2401 agaccaccgg cctgccgatc gccgagatcg ccttcgccgc cggattcgcc agcgtccggc
     2461 agttcaacga caccatcaag gagatctacg cgctcacccc gagcggactg cgggcggtcc
     2521 gccccgggcc cggttcccgc ttcggcggcc ttgcggcccc gtccggcacg cccggcgtgc
     2581 tgccgctgag gttggcgtac cgcggcccgt acgcggcccg gcagatcttc gactacctgg
     2641 cacagcgggc gctcggcggg atcgaggagg tcaccgggga gcccggtggc cggacgtacc
     2701 ggcgcaccct gcggctgccc cacggcaccg gcatcgcgga ggtcgacgag gccaccggcg
     2761 acggctggct ggactgccga ctgcacctca ccgagctgcg ggacctcgcc accgccgccc
     2821 agcgcgtccg ccgcctgttc gacctggacg ccgacccgtt cgcggtaggc gaacgcctcg
     2881 gtgcggatcc cgcgctggcc ccgttggtcg cccgctgccc cggactgcgc tccccgggga
     2941 ccgtggaccc ggacgaactc gcggtgcgcg ccgtcctcgg ccggcgggcc gggaccgccg
     3001 ccggtcgggc cctgggcaca cgcctggtcg ccgcgcacgg ggagctgctg ccacggcctg
     3061 ccggcaccct cggccatctc ttcccgcggg ccgtcgagtt ggcggacgcg ccgctcaccg
     3121 ggctcggcct gtccgacgcg cggatcgccg cgctgcgcgc cctcgccgcc gccctcgccg
     3181 cccgggacgt cgtcctggac gccgggaccg accgcgaccg ggccgaacgc gcgctccgcg
     3241 ggctccccgg catcggtccg tggaccgccg actacatccg gatgcgggcg ctgggcgacc
     3301 ccgatgtcct cctcggcgca ccgccggcgg gcaccgaccc ggcagtgtgg agcccctggc
     3361 gctcgtacgc gacgcaccac ctctgggccg ccgaccccgc cggcaccgcg gaacggcacc
     3421 cgaacccgac agaacggatc gacgcatgac cttgtacgcc accctcgaca gccccctggg
     3481 cgaactcctg ctggtcggcg agcggtccgc caccgcaccc ggcggcacgg cgctcgcctc
     3541 gctctccgtg ccgggtcaga agcgcggggc cgtcgtccag gacgactgga cgcacgaccc
     3601 ggccgcgttc gccgggatcg cccgccagct ccgggcgtac ttcgacgggg cgcagcagcc
     3661 gttcgccgtc gagtgcgtcc cccgcggcac ggacttccaa cagcgcgtct gggcggcgct
     3721 ggaggccatc ccgtacggca cgacgaccac ctacggagcg atcgccgcgg ccatcggggc
     3781 gtcgcgcggt gcggtccgcg ccatcgggac ggcgatcggc gccaacccgc tgctggtggt
     3841 ccggccctgc caccgcgtca tcggcgccga cgggtcgctc accggctacg ccggcggcct
     3901 ggcccgcaag caactgctcc tcggcctcga aggggcggcg ccggcggccc gttgagccgg
     3961 ccgaacacgg ccgcgcccgc cggtccgggc agccacccgg accggcggac cggttcggtc
     4021 agagcatcga cccgccggtg acgtcgatgc gctggccggt gatccaacgg gagtcgtcgg
     4081 aggcgaggaa ggcgaccacg tccgcgacgt cggcgggtcg gccgagccgg ttgaacaccg
     4141 agatggcggc aagtgccgca tgtgcctcgg gagtagaccg catcgccgcg ttcatatcgg
     4201 tctctatgaa tcccggcgcc acggtgttca ccgtgatccc gcgctcgccg acgtccttgg
     4261 cgagggtccg ggtgagggtg tcgatcgcgc ccttggagag tccgtaggcg atggtcgagg
     4321 ggaaggccgt actcgtcacc gcggacgaga tgttgacgat ccgcccgccg tcccgcatcc
     4381 gccgcagacc gtgctggatg atgaagaacg gggccttggt gttgatcgcg atggtccggt
     4441 cgaactcctc ggtcgtcacc tcgccgatgt gtcccggcag gctgatcgcc gcgttgttga
     4501 cgaggatgtc caggccgggt tccgcgcccg ccgcggccag cccggcgtcg aaggcgtcga
     4561 acagcgcctc ggcgtcgccc gggacgccca gctccgcacc gaccaggaac gcctgaccgc
     4621 cgttgtcccg gatgccctcg acggtctcct tggccgcgcc ctcgttgctc ccgtaatgga
     4681 cggcgaccac ggcaccgtcc tgtgcgaggc gctgggcgat gccccgcccg atgccgcggc
     4741 tggatcccgt aaccagcgcg gtctttccgt aaaggtcacc catggtcgtt gtcggccttc
     4801 cgtagtgctt ctggcggtcg gttgccgccg tgtcaaaggg gagtgctgtg cgggcggccc
     4861 ggccgttgtt ccggtatgtc gtctgcctac acggtgcatg agccggtcgg gctcatgacg
     4921 aaggggggtg gcgcgtgcgt gccggcgccc atgcggcggt gggctcggac gggcggttcg
     4981 atggcggccc gcccgtccgg tcagctgcgc gcatccatgg cggtcaggac ggaggtgaag
     5041 accggctcgc cgtcctgggc cccggtgacc aggaccgtcg tggtgccggt ggtcgtcggc
     5101 accttccgcc gcgcctcgat cagacacggg cggccgtgtt ccgcgtatcg gtggaactcg
     5161 gtgccgaagg cgtagggcag gaaggaactg ctcccggtca gcgtgcgggc ggcctgctgc
     5221 gccgcgtcga gcagggacag tccgggcacg tggtcgttgg cggggttgac gaccgtgggg
     5281 tggccgggcg cgatccgcag cagccatcgg cccggctgcc cgtcggggga gagcaggacg
     5341 tccctctcgg tggcgctgcc ggtcagtccc ggcgggacgg gggtggggag cgggccgggg
     5401 acgtggcggg ccgtgatccg ttccccgcgg acccggcggt agacggccgg cgaggtgacg
     5461 ctgacatggg agtggccggt cgcgacgacg cggccgtcct tgcggatcgt catgtcgaag
     5521 cggctgctgg acagcatgcc ggcgcgcatc cgtgtctggc tgcacacgac gtcgatgctc
     5581 agctgcgtcg gcccgttccc ggagacgcgg tgcgcggggt cggcggtgta gacgaggtcg
     5641 tgaaggatga agtgatgacc gagcggcacc ccgaactcgg tgtggcagag cagcagtccg
     5701 gcctgtcgga tggtctctcc cgccagcatc ggatggggcc ggttgtcgtg gggggcgaag
     5761 tagaggtgat catgcggcca ctgggcggtc acggagaagc ggtcctggct cagtcggagc
     5821 caggaaatga tcaggatgtc ctccacgagg gggcggtgca gaaactcgtg ggggatcggg
     5881 gcactggcgt cggacccccg ccggcttgcg gccggggggt tctggacctg ctgcatccgg
     5941 cgtcggctcg tgactggaac cgtgctgctg gcctgcaacg tgtcgctcat gacagcccct
     6001 atgcgtctct acggaagagc tccggacacg attaagatac gtaccatccg gttttctttt
     6061 caatgccggt gtgcgggcaa aggcgatccg tcgatctgga ggagacttga gctgtggcga
     6121 aatgctgaga agcttgagcc tttgacagac aggaggcgct ctgtggcacg tcaggaccgc
     6181 gcaatcaaga cgcggcgagt gatcttggaa tccgccgcgg cggtcttcgg tgaacacggc
     6241 ttcgaccgcg cgacgattgc cgagatcctc tcgcgcgccg gcgtgacgaa gggtgccttg
     6301 tatttccact ttccctctaa agaagatctt gcgctcgccg tgctgcatga gcaggtgctc
     6361 gacatcgccg tgcggccgca gccgatcaaa ctccaggagt tcgtggacgc cgggcagatc
     6421 ctggcatacc ggctgcgaca cgacccgatc cagcgcggcg ccgcccgtct cgccatcgag
     6481 ccgggtgcca accaactcga tcgcaagcag tcgatgtcgg cgtggaccac gttcgtcgag
     6541 ggtctgctca acgaggccag ggcgcggggc gaagtgctgg agagcgtcaa cgtgcgggac
     6601 acggcggagc tgttcgtcgg tgccttcgcc ggtattcaga tgatgtccaa ggcgttcacc
     6661 gaccagaacg acgtcagtca tcggctgttg gtcttcttcg agcacaccct gcccaccatc
     6721 gcggtgccgg ccgttctcgc caaactcagg ctcgacccgg agcggggtgc gcggctcgat
     6781 gcggagctcc gggaacgcgc ggcagaggag gcggagcacg ccgccgcgct ggaggcggac
     6841 ctgcagaacg cggcgccgta gcgcgccgcg ggagctcctc ggggtgcgga tgtcgcgggg
     6901 cggaagttcc ccgggtgtgc tgatacgtcg gttccgtccc gggcgcggcc ccgccgcccg
     6961 tgctccgctc ttgacccagt cttgatccct cgtccggtcg attgtcagtg gtgtgcggtt
     7021 cactggatcc cgtgtccgga acgagaccgg gggaggggcg atggacggga cggcgggccc
     7081 gaaggggcgg gccgcgtacg ggtgttggtg caggcggagg ccgggcgtgg tcggcgtgag
     7141 cgagatcacc gtccggggcc cgagatctgg ccggatggct tggaacaggc ccccgagctg
     7201 aaacgttcgc catgcacgca tcaaggggcc acgcccctgt tcgcgcgccg cgcaccctct
     7261 ccgctcgctc ctgccgccct ggaggccgca cgcatgaccc agacgatgac ggaccagctc
     7321 cccgcggggg ctgcggactc cgccgccgcg ccgccactgc ccccgcccgc caaggacgga
     7381 caccgcagaa cgcacgccgg catcgtcccg gtgctcgcct tcgccggcat cgtcgtcgcg
     7441 gtgatgcaga cgctgctcgt gccggtcatc aaggacctgc cgaccctgct cggcaccgcg
     7501 cccggcaacg ccacctgggt catgacggcc accctcctcg ccggtgcggt cgccaccccc
     7561 atcatgggcc gcctcggcga cctctacggc aagcggcgga tgctgctgac gagtctcgcc
     7621 gtcatggtcg tgggctcgct gatctgtggc ttcaccgacc agttgatcgt catgatcgtc
     7681 gggcgggcgc tgcagggctt cgcgatgggc gcgatacccc tcggcatcgg cctgatgcgc
     7741 gatgaactgc cccgggagcg gctcggctcc gccatggccc tgatgagctc ctcgatcggc
     7801 gtcggcggcg gactggcgct gcccgccgcg gccctggtgg cccaacacgc cgactggcac
     7861 acgctgttct tcgccgccgc cgggctcggc gtgctgtcga tgctgctcac cgcgctggtc
     7921 gtcccggaga cctcggtacg ggcgcccggc cgcttcgacg tcgccggcgc cctcggcctg
     7981 tcggtcggcc tggtcgccct cctgttgccg atcaccaagg cgagcgagtg gggttgggga
     8041 tcgccccgca ccctcggcct gttcggggcc gcggcgctga tcctggtgct gtggggcgtg
     8101 atggagctgc ggatcgccga cccgctggtg gacctgcgca ccaccgcccg gcgcgaggtg
     8161 ctgctcacca acctcgcctc catcacggtc ggagtggcgt tctacgccat ctcgctggtg
     8221 ctgccgcagc tgctccagtt gcccacgtcg accggctacg gcctcggcca gtcgatggtg
     8281 gtggccgggc tgtgcgtggc gccgctgggg ctgacgatga tgttcgtggc accgctgtac
     8341 gcacggatcg cggcccgccg cggccccaag gtctcgctgc tgctcggcat gctggtcatc
     8401 ggggtcggtt acggcggcgg gatcggcctg atgcaggccc cctggcagac ggtcgtcatc
     8461 gcggtggtgg tcggtgcggg catcggcctg gcgtactcct cgctcccggc gctcatcatc
     8521 ggggccgtcg acccgtccga gaccggcgcg gccaacggcc tgaacaccct gatgcgttcg
     8581 atcggcacgt ccgtgtccag cgccgtcatc ggcatggtgc tggcccgtat gtccgagccg
     8641 ctggggccga tcaccgtgcc cacgatggcc ggattccggg tctcgttcct gatcgccacc
     8701 gccgcggtcg ccctcggggt gctggtggcg gccttcctgc cgtcccagcg gaaggccgcc
     8761 ggggcgcggg cgacgccggt gacgcagagc gcggacgagg ccggcacggc ggcggacgtc
     8821 ccggcggacg cggccgggcg gagcgcaccc gctgcgggag cggcggacga gccggccgcc
     8881 gggttccggg ggcgggtcgt ggacaccgca ggcggtccgg tgccgggcgc cacggtcacc
     8941 ctgatcgacc ggcacgggca gcaggccggg gtcaccacgg ccgcgtccga cggcagttat
     9001 gcgctggccg ccccggccgc gggcacctac gtcctgaccg gcgccgcccc ggcacacgcc
     9061 ccgcaggccg tctccgccgc gcatgccggc gagggcacgg tggccggggc ggacctggtg
     9121 ctgacgccca ccggcagccg cctcgccggc accgtgctcg gtggcccgga agggacggcg
     9181 ctgaccggag cgcgcgtcgt gatcaccgat gcccacggcg acgtggtgac gcacacgacg
     9241 accggagccg acgggcggtg gacggtcgcc caactgccgg acgggcccta caccctcgtc
     9301 ttcagcgctc cggggcacca gccgaccgcg catgccgtcc agttgggcgg cggcgtcgac
     9361 ggcgccgggg gcgatacgcg ccaccaggag gtgcggctgc ggcccggcgg caccgtgcgc
     9421 ggtacggtcc gcgggccgga cgggcacccc ttggcggacg ccgccgtgac cctgctggag
     9481 gacgggacgg tcgtcgggca cgcggtcacc gggccggacg gcgcctatgc cttcgcggac
     9541 ctggtgggac gccactacac cctctcggcg gccggctatc cgccgcacgc cgcgccgata
     9601 tccctggcgg gcggcgcgga tgcgcggctg gacctggagt tggcgcagcc gggcggggcc
     9661 gggcagcggg agcgctgaga gtgccgcccg tggcgtgcgc cggcccgcgg tgattcctcg
     9721 tcagcgcgga gggccgctgc cgacggccgt gccgacgcag gcagtgccgg cggcaccgct
     9781 tccagcggcg ttcgcacaat cctgacgagg tgtcacccgg cttccgcggg gcgccccgtc
     9841 agcaagagcg cccgggtttc cgccagttcg gactggagga cgtcgccggc ggtgtcggca
     9901 tcgccggcgg ccacggcctc gacgagggcg gcgtggcggg cgtccccgtg gtgcgggtcg
     9961 tggtcgcgca ggccgagcag ccggacgagg tcgatcaggc cctggcgcag ggcggggacg
    10021 aactggccga agaggtcggt cagtacgggg ttgtgggcgg cgtccaccac cgccgcgtgc
    10081 agggcgatgt ccgcgtcgac gaaggctgcg tcgtcggcgc cgtccgcccg ccgcctgtcc
    10141 agtgccgcgt acagggccgt gacatcggcc tcggtgcggc gctcggcggc gaggcgggcg
    10201 gcctggacct cgaccatcgc gcgcacctca tagacgtcgg cgacggccgc gcggcgcagg
    10261 cgggtcggcc agtcctcggt gggttcggcg gcgatgacga agacgccggc gccctggcgg
    10321 ggctgcacca ttccggcgcc ggccagtgcg cgcagcgctt cgcggaccgt cgagcggccg
    10381 acgccgaggg tcttggccag cgtcgtctcg cccgggagcc tgctgcccac cggccagtgt
    10441 ccgccggtga tctggtcgcg cagctgttgc gcggcctgtt cgaccagggg gctggggcgg
    10501 agggcttcca gcggcatccc gtttctcctg tcctcataac tcctcagctt gtctgaggag
    10561 ttgattcgtg gttagtgtac ccgccatgac gctgcgtggc ctcctcctcg ggtgccgcgg
    10621 cggggcctga cacgaccggc accccgccgc ggggttcggt gctgccggtc tctccccccc
    10681 ccggcttctg tgaaggaacg cgatgtcccg cactttcccc accgtccgca cgccccgcgg
    10741 gccggtgccc gcgacggccc cggcctggaa tccgcagcgc ggcagcgcca tgccggccca
    10801 ccgctaccgg cccgcccacc agcgggtcga ggtgccgcac accgaccgcg cgtggcccgc
    10861 cgcccggatc gagcgggcgc ccctgtgggt gccggtcgac ctgcgcgacg gcaaccaggc
    10921 gttggccgaa ccgatg
//
