LOCUS       NZ_CP054019            22535 bp    DNA     linear   CON 28-JAN-2024
DEFINITION  Streptomyces sp. A1-5 chromosome.
ACCESSION   NZ_CP054019
VERSION     NZ_CP054019.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. A1-5
  ORGANISM  Streptomyces sp. A1-5
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 9459690)
  AUTHORS   Dashti,Y., Sumang,F.A.M., de los Santos,E.L.C., Alcantara,E.P. and
            Challis,G.L.
  TITLE     Structural revision and biosynthesis of polyketides FXJ15321 and
            FXJ15322 from Streptomyces sp. A1-5
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9459690)
  AUTHORS   de los Santos,E.L.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2020) Warwick Integrative Synthetic Biology
            Centre, University of Warwick, Gibbet Hill Road, Coventry CV4 7AL,
            United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: JUN-2019
            Assembly Method                   :: Flye v. 2.4.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Oxford Nanopore MiniION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/28/2024 04:28:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,328
            CDSs (total)                      :: 8,230
            Genes (coding)                    :: 7,847
            CDSs (with protein)               :: 7,847
            Genes (RNA)                       :: 98
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 156 of 383
            Pseudo Genes (incomplete)         :: 280 of 383
            Pseudo Genes (internal stop)      :: 26 of 383
            Pseudo Genes (multiple problems)  :: 73 of 383
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 22:08:01
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 7488606
            Orig. end                         :: 7511141
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP054019.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Philippine National
            Collection of Microorganisms.
FEATURES             Location/Qualifiers
     region          1..22535
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="lassopeptide"
                     /region_number="27"
                     /rules="(PF13471 and Asn_synthase or micJ25 or mcjC)"
                     /tool="antismash"
     cand_cluster    1..22535
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /kind="single"
                     /product="lassopeptide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..22535
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:12535](+)"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..12535
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
     misc_feature    complement(51..65)
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 19.74"
                     /tool="antismash"
     misc_feature    complement(96..108)
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 19.11"
                     /tool="antismash"
     misc_feature    392..407
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.28"
                     /tool="antismash"
     gene            complement(<523..717)
                     /locus_tag="HRD51_RS32915"
                     /pseudo=""
     CDS             complement(<523..717)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016472561.1"
                     /locus_tag="HRD51_RS32915"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="TetR family transcriptional regulator"
                     /pseudo=""
                     /transl_table=11
                     /translation="MSQGGEKASAGGARGLRADAERNRARVLSAARQVFLESGTAAPLD
                     EIARRAGVHIATLYRRFPDR"
     gene            complement(765..3677)
                     /locus_tag="HRD51_RS41940"
                     /old_locus_tag="HRD51_32505"
     CDS             complement(765..3677)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /locus_tag="HRD51_RS41940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HRD51_32505"
                     /product="CHAT domain-containing protein"
                     /protein_id="WP_268826609.1"
                     /transl_table=11
                     /translation="MSSELARAKALFEQGRDLFLRGRYQDAVRLLAAAAELAPTSVGCQ
                     FMLGAGRWNSGDTAGALYPLALCVYLDSEHASGRFALGVALRALGQPEQGAFHVAYAAY
                     RGNRQAAAHLRELALAHCPDCGGPVTSGAARCDGCAGIPREISAPRPRTWSWSHLRSDD
                     ARSRWTLPYEQLADLGRGHLTRYAGHGDPRDIDDALAYLELAAATASAADRPDRLTELG
                     SAYRRRFACHGLPADLDHAIDCHEQALDQVAADTPEQVIVLANLGVAYGARHESSAAAA
                     DLDRAIGFLERAMAAPTEDPDEQLSAMASAGVFYQRRHRAGGDEADLDRSIALQDAVVE
                     ASPPDHPRYAGRTANLTVAYLNRYECHRRLPDLERALLLADRAVAATPRDSPALPIRLA
                     IRGSTWLQLHLADLPGESGGTRDRPLDRALDDLRRALDAVPDGQPHLADVLAELVRAHL
                     VPGARHLAPDRRTMEAWEARLKAARGATPSRRAVAGRNLGVLANVLGAYDLAARVLDAA
                     IALTPAVPLRGTDWTDQERRFGEPRGLASQAFAAHCARGEPLQAVQQAELGRGIQLATV
                     LDAHDDLADLESELPELARPFRQVRKELAAGSAAEAVLWERYGQLVARIREHPRFTRFL
                     MTPRVEELRQAAGGGTVVVVNSARNRADAILIGHDGDLKQVPLPDLTADHVEAQSEAVV
                     GSAHAGLADRLGWLWDTVVGPVAEALPPGAGPRRVWWLPIGLLGMFPLHAAGHPGRPGA
                     LDSFVSSYTPTLRILAHAHGHPAAVARRQLTVALPSTPGLPGLPGALTEALDLHRRHPD
                     QPPLLNEDATTSQVAAALSTATWAHFACHALADHAAPSHSGLCLSDGMLTIPEISRLAV
                     ADAQLAYLSACSTGHRSLEHVDESLNLASAFQLAGFRHVIASLWPLADAFGAVAARTFY
                     AELSGTADDAPGALHRTTLAMRDRYQGRPDLWASLIHSGP"
     misc_feature    complement(3336..3338)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(3674..4291)
                     /locus_tag="HRD51_RS32925"
                     /old_locus_tag="HRD51_32510"
     CDS             complement(3674..4291)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="HRD51_RS32925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="HRD51_32510"
                     /product="hypothetical protein"
                     /protein_id="WP_235094890.1"
                     /transl_table=11
                     /translation="MREQAVCDTCGTTTRRSSGYHLPTKHVVVSEAYWRSFFRTAVGLV
                     RALNWDERAQAGAFDRLISQSASSATPWLVCEECSEWFVFDRAAAREHARRGSVPESSG
                     AVDPAGFAPFAAAAWEYVVGRWPASVQQPTVGDTCDLCAKKIYQGELVGRIGAGTAEAY
                     LASGVLETPPLSPPRPDQQGWLACWVCVSRLQTRAGRARGGR"
     gene            complement(4321..5985)
                     /locus_tag="HRD51_RS32930"
                     /old_locus_tag="HRD51_32515"
     CDS             complement(4321..5985)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF022432.4"
                     /locus_tag="HRD51_RS32930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HRD51_32515"
                     /product="DUF2786 domain-containing protein"
                     /protein_id="WP_235094891.1"
                     /transl_table=11
                     /translation="MVAPGGPSEPKDAPSISRRLQSLRALAEHPRTPRAERELAAALAA
                     ALMERYGLGAPERRDGTARDGIIVVRMEPDKTVEVLVLAPDAERATRQIAGIMGRDGGA
                     PASWNVHRLNAQGLNPGIAVWRKAGALAQQRAVNFMASNITRRGGDLGGLGSIGGTTVV
                     TGYDTATGALASTPLESLEHLLEMEAYRDAAAQLRAWRTERGITTRPTATRPRPTALRV
                     LSVEEVVRLTPCAPWEARLQAEIWVEGDRLPHVRRPRVGVDRPPHPGHPHGARRRLLTG
                     TGVPARRPPRQRRYAPGAAPPARETRRDRMTAHVPPAGADTTADLASGALARARAAGDR
                     RAEARALCDLGAALTRAGRASEGRYLCRRAAGLAAEVGDRPAEEAAFGALGHNLAELDA
                     LGERWRAVLLEGGDETGFFASPAAARSDASYATGGRPDDAPARLTGVPVVELACGFVAV
                     KFLGPFVESFAGKLGERLGESTVTATGRLRLLWHRRSGRRDLDVLTPGERTTLVLPDDF
                     DDAARLAAIDLDLGADGVRGAELHWDADAGTWSPVNT"
     gene            complement(<6655..6858)
                     /locus_tag="HRD51_RS32935"
                     /pseudo=""
     CDS             complement(<6655..6858)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /locus_tag="HRD51_RS32935"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="VOC family protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MEERAPGRPRSRTRLWLNPAQGALRAEVDRLTGLGARVVERRWTN
                     RSCGLEVVVLADPEGNEFCVESS"
     gene            6879..7178
                     /locus_tag="HRD51_RS32940"
                     /old_locus_tag="HRD51_32525"
     CDS             6879..7178
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF025789.4"
                     /locus_tag="HRD51_RS32940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HRD51_32525"
                     /product="SUKH-3 domain-containing protein"
                     /protein_id="WP_268826610.1"
                     /transl_table=11
                     /translation="MRFITTYGLLNLTHPSDPESKLITNPTGGYEGDFGEIADLSRELG
                     KRLFRIGYDLPDAGIFVVAEDGEFYCIHHAEAHYLGSDEFEFFGNWVKGDMQSV"
     misc_feature    6880..6894
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 17.29"
                     /tool="antismash"
     misc_feature    6881..6894
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 13.93"
                     /tool="antismash"
     misc_feature    complement(7378..7395)
                     /note="TFBS match to HipB, Bacterial antitoxin HipB,
                     confidence: weak, score: 21.71"
                     /tool="antismash"
     gene            7596..8258
                     /locus_tag="HRD51_RS32945"
                     /old_locus_tag="HRD51_32530"
     CDS             7596..8258
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /GO_process="GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HAD_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017946215.1"
                     /locus_tag="HRD51_RS32945"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HRD51_32530"
                     /product="HAD-IA family hydrolase"
                     /protein_id="WP_235094893.1"
                     /sec_met_domain="HAD_2 (E-value: 1.6e-09, bitscore: 37.0,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MARGTDGCTPYRGLILDFVGVLTEGVQAALHSWCASEGLAPDAWR
                     ITLADLPEGRELYAALESGRLSQAEWNRRTAPLLGVEDHHDLMGRAWSGVRPAVNMIAL
                     ARAARRAGYTVALLSNSFGLDPYDPYEALGVWKLFDVTVISEREGIAKPDPEIYQRTLE
                     RMDLPGEACVFVDDNPGNLPPAAALGITTVHADGQADTVKRLSSLLGILPAAGRVHR"
     gene            complement(8200..9210)
                     /locus_tag="HRD51_RS32950"
                     /old_locus_tag="HRD51_32535"
     CDS             complement(8200..9210)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1014: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014049074.1"
                     /locus_tag="HRD51_RS32950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HRD51_32535"
                     /product="LysR family transcriptional regulator"
                     /protein_id="WP_235094894.1"
                     /transl_table=11
                     /translation="MDTEALRSFVRAAELGQFQHAADELGVTQQAVSKRIATLERELEV
                     RLFTRTARGVELTLDGQAFLSHARSIVAGVERAINAVRPGSRALRIDVLGLRSAQAVVL
                     HDYWRSRPEANLDVVTLRANDPRVVVAAVEAGDIDASFRTVIAPAVLPPALQMIHAFDS
                     PLELLVGPRHPLASARRLTPPQLRRHRIWVPGIAPRSEWAEFYDQLATDFDLRIDATGP
                     HFGDEVLLDTLADSADVATLVGARDRYIWPTNHDLRRIPIVNPTLAYPLSLILPRTNPH
                     PELRGIITHLGTLPRLPDAVWRPSWATTPQRGESGGTTCELCRRPGESPADSTAA"
     gene            9812..9961
                     /locus_tag="HRD51_RS32955"
                     /old_locus_tag="HRD51_32545"
     CDS             9812..9961
                     /codon_start=1
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF033521.1"
                     /locus_tag="HRD51_RS32955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HRD51_32545"
                     /product="keywimysin-related RiPP"
                     /protein_id="WP_107080297.1"
                     /transl_table=11
                     /translation="MQGNELQETDKLQTYEAPKMIECGSFQEDTGYFGLTGYENLFHFY
                     DKLH"
     CDS_motif       9812..9904
                     /prepeptide="leader"
                     /note="peptide class: lassopeptide"
                     /note="predicted sequence: MQGNELQETDKLQTYEAPKMIECGSFQEDTG"
                     /locus_tag="HRD51_RS32955_lassopeptide"
                     /aSTool="lassopeptides"
                     /tool="antismash"
     CDS_motif       9905..9961
                     /prepeptide="core"
                     /locus_tag="HRD51_RS32955_lassopeptide"
                     /peptide="lassopeptide"
                     /predicted_class="Class II"
                     /score="2.20"
                     /molecular_weight="2246.5"
                     /monoisotopic_mass="2245.1"
                     /core_sequence="YFGLTGYENLFHFYDKLH"
                     /aSTool="lassopeptides"
                     /tool="antismash"
                     /leader_sequence="MQGNELQETDKLQTYEAPKMIECGSFQEDTG"
                     /leader_location="[9811:9904](+)"
     gene            10001..11821
                     /locus_tag="HRD51_RS32960"
                     /old_locus_tag="HRD51_32550"
     CDS             10001..11821
                     /GO_function="GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA]"
                     /GO_process="GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: Asn_synthase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1177: asparagine synthase (glutamine-hydrolyzing)"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /locus_tag="HRD51_RS32960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HRD51_32550"
                     /product="asparagine synthase-related protein"
                     /protein_id="WP_235094895.1"
                     /sec_met_domain="Asn_synthase (E-value: 2.3e-39, bitscore:
                     135.0, seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MADAGWQYFVALPDDEGAAAAIGRFPTDGVRVLLRHRSGRPCVAG
                     RLAEDRVLVVERGDTRLAVIGHCSATRDELATAADRIDDLTDLDRLGLAWAGSYHLVAS
                     VRGRLRVQGTASGLRRIFHGELGTLTIAADRADVLATVLDAPLDPALLALRTLDALPHP
                     LGDLPPWRHVSAVPPGWYLALPQDGRRPLVERWWQAPEPVRSLTAGGQLLRQALEDAVR
                     VRARGARPISADLSGGMDSTSIALLAVRHRPSLTVLTMENDDRADEDARWARQAAAGLP
                     DLNHIVYSSRELPRFFGGLTAVEGVPDEPSTAVLSAPRLRAVRQRALAHGSRLHLDGLG
                     GDQLLCGHPAYHHDLLRHRPLLAAQRLRVLTQLRRSNWGTEARGLLEGHSYRRWFTDRA
                     HGRALRTPSPLSAWGHFPGLPAWLTPDARDLVTRTLRDTAATVEPLASTRGRHNDLLAI
                     QDAGRMVRQCHQLTETGDFSHTSPFLDDRVIQACLAVRPEQRVTPWEFKPLIKAAMADV
                     MPPEVLTRRTKGDGTTIVAEGFEAHRRELAELWDDSRLAALGLVDPGPLRELFDLPYTN
                     SLHQGAMQSALSCELWARATAFQPAAATRP"
     aSDomain        11853..12104
                     /aSDomain="Stand_Alone_Lasso_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="Stand-alone RRE protein in a lasso peptide
                     cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_HRD51_RS32965_Stand_Alone_Lasso_RRE.1
                     "
                     /evalue="1.50E-27"
                     /identifier="RREFam002.1"
                     /label="HRD51_RS32965"
                     /locus_tag="HRD51_RS32965"
                     /protein_end="84"
                     /protein_start="0"
                     /score="86.4"
                     /tool="antismash"
                     /translation="MTWRLHPDVSTVDTDYGTVLLNGSDGRYWQLNATASLAFRALVEG
                     STVQEATERLMASFDVDEPRARADVEQLMASLRQAKVVQ"
     gene            11853..12113
                     /locus_tag="HRD51_RS32965"
                     /old_locus_tag="HRD51_32555"
     CDS             11853..12113
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: Stand_Alone_Lasso_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF05402"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004949363.1"
                     /locus_tag="HRD51_RS32965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HRD51_32555"
                     /product="lasso peptide biosynthesis PqqD family chaperone"
                     /protein_id="WP_205369049.1"
                     /sec_met_domain="Stand_Alone_Lasso_RRE (E-value: 4.5e-25,
                     bitscore: 86.4, seeds: 25, tool: rule-based-clusters)"
                     /sec_met_domain="PF05402 (E-value: 9.7e-14, bitscore: 50.3,
                     seeds: 461, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTWRLHPDVSTVDTDYGTVLLNGSDGRYWQLNATASLAFRALVEG
                     STVQEATERLMASFDVDEPRARADVEQLMASLRQAKVVQQA"
     gene            12110..12535
                     /locus_tag="HRD51_RS32970"
                     /old_locus_tag="HRD51_32560"
     CDS             12110..12535
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: PF13471"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004949361.1"
                     /locus_tag="HRD51_RS32970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HRD51_32560"
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="WP_189863786.1"
                     /sec_met_domain="PF13471 (E-value: 2.3e-26, bitscore: 91.0,
                     seeds: 84, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSSVVPHRTEVRLSPGRRLLVLLAVAVARRLAAKPPARTRAILTR
                     LRRGARAATYAEARAARDAVVAVSLRCAGDEGCLPRSLATVLLCRARGTWPTWTVGVRA
                     RLPFGAHAWVEAEGRMVEEKADPSYFRPLFSVPAEGA"
     tRNA            complement(12635..12708)
                     /anticodon=(pos:complement(7501278..7501280),aa:Met,
                     seq:cat)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="HRD51_RS32975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="HRD51_32565"
                     /product="tRNA-Met"
     gene            complement(12635..12708)
                     /locus_tag="HRD51_RS32975"
                     /old_locus_tag="HRD51_32565"
     gene            12958..14685
                     /locus_tag="HRD51_RS32980"
                     /old_locus_tag="HRD51_32570"
     CDS             12958..14685
                     /EC_number="2.7.11.1"
                     /GO_function="GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pkinase"
                     /gene_functions="regulatory (smcogs) SMCOG1030:
                     serine/threonine protein kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018539792.1"
                     /locus_tag="HRD51_RS32980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HRD51_32570"
                     /product="serine/threonine-protein kinase"
                     /protein_id="WP_235094896.1"
                     /sec_met_domain="Pkinase (E-value: 3.2e-47, bitscore:
                     160.0, seeds: 54, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSGEGTERAVTAGGASGEELIGKVLGGRYRVTATIGRGGMGVVAR
                     AVDQLLNREVAVKVLRAFTDASAPELADLRTRMQREAQAAARIRHSGVVTVHDVTEEQG
                     LPVIVMELVDGPSLDDVLTERGTLEPHEAAAIGAKLMDALDAAHRVGVLHRDVKPANVL
                     LEHGGRVVLTDFGIASMDASGDEAMAKLTHSGQLVGSLDYLPPERARGTVPSPASDIWS
                     LGMTLYAAVEGTSPFRRTSVWSTLSAIVTEPLPEPRRAGPLTPVLQALMAKEPKNRPTA
                     EQAREMLQWAAAGRTMNLAPPAPMAPAPPPVQAVGPTPPQAATPAPPPGFGAVPPLHPS
                     SPQPAHTGYPPSVPPPVTQPCMPTAGHAHGAGPAAGRGRRSRRTLIAAAAAFVVLAGGG
                     VTYALTAQPGAADGNGPQAAPGASSPVGSGQPSGGGTADGINSPGSSPSTGSGNPSGAT
                     TPSSAPPSTKPGATPSTSASPSAKPSSGPTTCSGWSHHDPKPGTYGYLSGDHHILTGPY
                     QVCSSVAATTSGTKLWFHCSVENAYGHKWLYVRIGGTKNAGWMSADNVIRQSGWPVRC"
     gene            complement(14765..15301)
                     /locus_tag="HRD51_RS32985"
                     /old_locus_tag="HRD51_32575"
     CDS             complement(14765..15301)
                     /GO_function="GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA]"
                     /GO_function="GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354813.1"
                     /locus_tag="HRD51_RS32985"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HRD51_32575"
                     /product="carbonic anhydrase"
                     /protein_id="WP_328285882.1"
                     /transl_table=11
                     /translation="MALFITCADSRVVPTMFTGARPGELFELRVAGNIVPRYRPQAACG
                     VAATLEYAVTALHVPDIIVCSHSHCAAVQGLMHPRQLSFLPLLEKWLSLSGHGTRAAGE
                     PPTARTARVTHSDRPDPDTVAQLHTLIQLGNIRSYPFAATRIAAGKLRLHGWFYAVETG
                     QVYVEHPGSCRFQPL"
     misc_feature    complement(15218..15220)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(15963..16877)
                     /locus_tag="HRD51_RS32990"
                     /old_locus_tag="HRD51_32580"
     CDS             complement(15963..16877)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1014: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016571522.1"
                     /locus_tag="HRD51_RS32990"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HRD51_32580"
                     /product="LysR family transcriptional regulator"
                     /protein_id="WP_205369046.1"
                     /transl_table=11
                     /translation="MELTQRILQQIVVLAEEQHFGRAAERLAMRQPPLSQAIQRLERGL
                     GVALFERLPRGARLTPAGRAFAEDARRLLEAQAAAVDRARRIASGHEGELRLGFISSLG
                     YDFLPRLLRRTHAQLPDLRLHLGQHPSAELVDMLQAGTLDLALVRLPVTGTERLTVRRI
                     GVERLMAVLPEHHPLVATSRLDLRELAEEQFAVPKPGALPGLAEQVALACAQAGFTPRP
                     RGQADDLPGLLSYVAAGLCLALAPEQVRTLGIPGVVYRPLHGDSPYLESTVAAVHRADG
                     PDAAVQQVLKLIEPDLPTVEYTP"
     gene            16999..18180
                     /locus_tag="HRD51_RS32995"
                     /old_locus_tag="HRD51_32585"
     CDS             16999..18180
                     /EC_number="2.8.3.-"
                     /GO_function="GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1189: L-carnitine dehydratase/bile acid-inducible"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016571523.1"
                     /locus_tag="HRD51_RS32995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HRD51_32585"
                     /product="CoA transferase"
                     /protein_id="WP_235094898.1"
                     /transl_table=11
                     /translation="MSTGRETDGGVGPLDGVRVVELASVIMGPYAAQQLGDLGADVIKV
                     EPPTGDLTRHYPPTRNPGMGVMTLNLNRNKRSVALDLKAPGGRDALLALLASADVFLTN
                     VRPQALRRLDLAYEDVAAVNSGLVYLNAQGFRSDSPLGGHAAYDDVVQAASGLVWLNEQ
                     VSGVPHYVPTVLADKICGLVIVQSVLAALHHRDRTGQGQHVEVPMADTMLAFNLVEHLA
                     AATLSPPEGPIGYPRALSPRRTASRTADGWMCILPYSDRNWRDFFAFVGRPELADDPRF
                     ASYAARVHNADELYVLVGEFTIRHTNAQWQEFCDATSIPAAPVLSLTEAATSAYAAEGG
                     LLREAEHPTEGGYRLIGQPVRFWATPAGLHRHCPRVGEHTEEVFAEIGHRPPS"
     gene            18240..19067
                     /locus_tag="HRD51_RS33000"
                     /old_locus_tag="HRD51_32590"
     CDS             18240..19067
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: ECH (24-263). E-value: 1.2e-59. Score:
                     193.6. Matches aSDomain:
                     nrpspksdomains_HRD51_RS33000_ECH.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1023: enoyl-CoA hydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016571524.1"
                     /locus_tag="HRD51_RS33000"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HRD51_32590"
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="WP_235094899.1"
                     /transl_table=11
                     /translation="MNEQAPLDEQAGPNAWEPLRVEHRPNGVTVLTFDNPDKRNPMTGR
                     LTAQWRRAMRSLRQDPDLRALVITGTGPAFCSGADLGELADRHGQPLSGTRAQLSDFYR
                     TWLTVRDLSVPTVAAVNGHAVGAGLALALACDLRYAAAEAKLGAPFVRLGLHPGMATTA
                     LLAEAVGPSRARELLLTGRLISGARAATIGMVHEALPADQVLRAALDVADEIAAAAPLA
                     VRLTKSGLAQGQPTVERALTWEGLAQSVTTTTDDFREGVQARRERRRPHFTGA"
     aSDomain        18312..19028
                     /aSDomain="ECH"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_HRD51_RS33000_ECH.1"
                     /evalue="1.20E-59"
                     /label="HRD51_RS33000_ECH.1"
                     /locus_tag="HRD51_RS33000"
                     /protein_end="263"
                     /protein_start="24"
                     /score="193.6"
                     /tool="antismash"
                     /translation="PNGVTVLTFDNPDKRNPMTGRLTAQWRRAMRSLRQDPDLRALVIT
                     GTGPAFCSGADLGELADRHGQPLSGTRAQLSDFYRTWLTVRDLSVPTVAAVNGHAVGAG
                     LALALACDLRYAAAEAKLGAPFVRLGLHPGMATTALLAEAVGPSRARELLLTGRLISGA
                     RAATIGMVHEALPADQVLRAALDVADEIAAAAPLAVRLTKSGLAQGQPTVERALTWEGL
                     AQSVTTTTDDFREGVQA"
     gene            19163..20329
                     /locus_tag="HRD51_RS33005"
                     /old_locus_tag="HRD51_32595"
     CDS             19163..20329
                     /GO_function="GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /GO_function="GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_M"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1006: acyl-CoA dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016571525.1"
                     /locus_tag="HRD51_RS33005"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HRD51_32595"
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="WP_235094900.1"
                     /sec_met_domain="Acyl-CoA_dh_N (E-value: 5.4e-22, bitscore:
                     77.5, seeds: 790, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_M (E-value: 1.6e-19, bitscore:
                     68.8, seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_1 (E-value: 8.4e-08, bitscore:
                     31.5, seeds: 49, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTLSDLDLGAVQLPPEATRLRQEVRDFLAAERAAGTVLGRPDSWL
                     TGWDPAFTRRLARRGWVGMTLPRAYGGGGRSNLERYVVIEELLAAGAPVSAHWVADRQA
                     GPTILRHGTEAQRNHFLPRIAAGACFFSIGMSEQDSGSDLASVRTRAERTANGWRLTGT
                     KMWTGGAHVNDHAIVLARTDESEDRHAGLSQFIVDLRDPHVRVEPIRLLSGEHTFNYCH
                     LEGVELTDEALLGRPGDGWRQVTGELALERSGPERFLSTLPLLSALVGQLQRTGACSPE
                     QQARVGRLTARLASIRQLSLGVAAALAAGRPADVQAALVKDLGTRFEGELVNEVREILP
                     IAARFDATRGSYPELLATAILHAPGYTLRGGTNEILRGIITRGLELSR"
     misc_feature    19220..19222
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            20326..21333
                     /locus_tag="HRD51_RS33010"
                     /old_locus_tag="HRD51_32600"
     CDS             20326..21333
                     /GO_function="GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /locus_tag="HRD51_RS33010"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HRD51_32600"
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="WP_235094901.1"
                     /sec_met_domain="Acyl-CoA_dh_1 (E-value: 3.1e-14, bitscore:
                     52.4, seeds: 49, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSIEPCAESVAEPLSERAVDALPPLDTAPTALVALAREAGYRSTP
                     APLAETLLVARPLLAAAGLAVPDGQLTVAAGPLDVRFTHVDTDSRVGPSGAAGTDDALR
                     VSGTLHRVPWARAAEAAVVLTEGPGGGPVLFLLEATEWRQVAGTNLAGEPRDDLVLLDV
                     AVPAERVRRVPAALVREATWRSGLARAALIAGAARRCVELTVDHTSSRVQFGRPLSHFQ
                     AVKQEEAKLVEEAALVAAAVEAAASALSEPDSAEFTAWLAAAQAAASVAEITRIAHQLH
                     GAIGFTELSPLRLATTRMWSWRDEDSGERAWQQRIGRHVLDGGSGGLWQQVAGG"
     gene            complement(21346..21858)
                     /locus_tag="HRD51_RS33015"
                     /old_locus_tag="HRD51_32605"
     CDS             complement(21346..21858)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1278: PadR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018538119.1"
                     /locus_tag="HRD51_RS33015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HRD51_32605"
                     /product="PadR family transcriptional regulator"
                     /protein_id="WP_205369041.1"
                     /transl_table=11
                     /translation="MLELAILGFLAEGPLHGYQLRHRIAHLSGHARPVSDGSLYPAINR
                     LVRAGLLDRRTEPGTSAAQRHTLSLTDAGRRELVRRLRDANGLDITDSTRYFTVLAFLS
                     QLPDTTDQHAVLRRRLEFLEQPASFFHDGDRPLRSDDTDDPYRRGMLTIARATSRAERS
                     WLREVLA"
     misc_feature    complement(22358..22378)
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 20.3"
                     /tool="antismash"
ORIGIN
        1 ggagggggac ggggagggga agggggacgg ggatgacgtg accgtctctc tggtacagat
       61 cttccgcgtc cgcgacggga agatcgctcg cctgcgtgac tacttcacgc ccgagctgat
      121 gggctgaggg cgaccgaaac gacgagtgat cacttggttc caggaaacca ggcgcatgag
      181 ttccggcaat cgtgtcacgc accatgagga cgggcagacc tggctcctgc gcccggtgcc
      241 cggtgcccga gggtttctct cgcctgacgt ggcgcggaag aacgagcccg catcagcgag
      301 gacgacgccg aacgcgtgga cgccgggacc acggcagcac tgctgcgccg cacgctcgac
      361 ggccgccgcg atgaccgtcg gcaccctcgc cgacaccaag ccgtcacggc cagcctgccg
      421 cagtcccgtt gggggttcga ctgcgcggcg cggcaggcca cttgcgcggt ctccaggacg
      481 cgccggaacc cgtcgaggac gacctggcgg atcagcgccc ccccgatccg ggaaacgccg
      541 gtacaaggtg gcgatgtgca ccccggcacg gcgggcaatc tcatccagcg gagcggcggt
      601 gcccgattcc aggaacacct ggcgcgccgc gctcagcacc cgggcacggt tacgctcagc
      661 gtccgcacgc aagccccgag caccgcccgc tgaggctttc tcgccaccct gcgacatggg
      721 cgaagtcacc gtgcctcctg ggccggcccg cgccacgcgt cgggttacgg gccgctgtgg
      781 atgagcgagg cccacaggtc gggccggccc tggtaacggt cgcgcatggc gagcgtcgta
      841 cggtgcagcg ccccgggggc gtcgtcggcg gtgccggaca gttcggcgta gaaggtgcgg
      901 gccgccaccg cgccgaaggc gtcggccagc ggccacaggc tcgcgatgac gtgccggaag
      961 ccggcgagct ggaaggcgga ggcgaggttc agcgactcgt ccacgtgctc caggctgcgg
     1021 tggccggtgg agcatgccga caagtaggcg agctgcgcat cggccacggc gagccggctg
     1081 atctccggga tggtgagcat gccgtcactc aggcacagcc cgctgtgcga gggtgcggcg
     1141 tggtcggcga gcgcgtggca cgcgaagtgc gcccaggtgg cggtggacag cgcggcggcg
     1201 acctggctcg tggtggcgtc ctcgttgagc agcggcggct ggtcgggatg gcggcggtgc
     1261 aggtcgaggg cttcggtgag ggcgccgggc agcccgggca ggccgggcgt gctcggtagg
     1321 gcgacggtca gctgccgccg ggccaccgcc gcggggtggc cgtgggcgtg cgcgaggatc
     1381 cgcagcgtag gggtgtacga ggacacgaac gagtccaggg cgccgggccg gccggggtga
     1441 ccggcggcgt gcagcgggaa catcccgagc agcccgatgg gcagccacca gacccggcgc
     1501 ggccccgcac ccgggggcag cgcctccgcg accggcccga cgaccgtgtc ccacagccag
     1561 ccgagccggt ccgccagccc ggcatgcgcg gagccgacca cggcctcgga ctgggcctcc
     1621 acgtgatcgg cggtgaggtc cggcagcggc acctgtttga ggtcgccgtc gtggccgatc
     1681 aggatcgcgt cggccctgtt ccgtgcggag ttgacgacga cgaccgtgcc gccgcccgca
     1741 gcctgccgca gctcctcgac gcggggcgtc atgaggaaac gggtgaaccg ggggtgctcg
     1801 cggatccggg cgaccagctg cccgtaccgt tcccacagca cggcctcggc cgcagacccc
     1861 gcggcgagct ccttgcgtac ctggcggaac ggccgagcca gctccggcag ttcgctttcc
     1921 aggtcggcga ggtcgtcatg ggcgtcgagt acggtcgcca gctgaatgcc gcggcccagc
     1981 tccgcctgct ggaccgcctg gagcggttcc ccgcgggcgc agtgcgcggc gaacgcctga
     2041 ctggccaggc cccggggctc gccgaaccgc cgctcctggt cggtccagtc ggtgccgcgc
     2101 agcggtacgg cgggcgtcag cgcgatggcg gcgtccagca cgcgggcggc caggtcgtac
     2161 gcgcccagga cgttggcgag tacgcccaga ttccgcccgg ccaccgcccg cctgctcggc
     2221 gtggccccgc gcgccgcctt gagccgcgcc tcccatgcct ccatcgtccg tcggtcgggc
     2281 gccaggtgcc gcgcgccggg gacgagatgc gccctgacca gctcggcgag gacgtcggcc
     2341 aggtgcggtt gaccatcggg cacggcgtcc agcgcccgcc gcaggtcgtc gagcgcgcgg
     2401 tccagcggcc ggtcgcgcgt gccgcccgac tcgcccggca ggtccgccag atggagctgc
     2461 aaccaggtgc tgccccggat ggccagccgg atcggcaggg ccgggctgtc gcgcggcgtc
     2521 gccgccaccg cgcggtcggc gaggagcagg gcccgttcca ggtcggggag gcgacgatgg
     2581 cattcgtagc ggttcaggta ggccaccgtg aggttggccg tccggccggc gtatcgggga
     2641 tggtcgggcg gggacgcctc gacgacggcg tcctggagtg cgatcgaacg atcgagatcg
     2701 gcctcgtcgc cgcccgcccg gtggcggcgc tggtagaaga cgccggcgct ggccatcgcg
     2761 gacagctgct cgtccggatc ctcggtgggg gccgccatcg cccgttcgag gaagccgatg
     2821 gcccgatcca ggtcggcggc ggccgcggag gactcgtggc gggccccgta ggcgacgccg
     2881 agattggcga ggacgatcac ctgctcgggg gtgtcggcgg cgacctggtc cagggcctgt
     2941 tcgtggcagt cgatcgcgtg gtccaggtcc gcgggcagcc cgtggcaggc gaagcgccgg
     3001 cggtaggcgc tgccgagttc ggtgaggcgg tcggggcggt cggcggcgga ggcggtggcc
     3061 gcggccaact ccaggtatgc gagggcgtcg tcgatgtcgc gggggtcgcc gtggcccgcg
     3121 taacgggtga ggtggccgcg gccgaggtcg gccagctgct cgtacggcag tgtccaccgc
     3181 gagcgggcgt cgtcggagcg caggtgggac cacgaccagg tgcgcgggcg cggcgctgag
     3241 atctcccgcg gaatgcccgc gcagccgtcg cagcgggccg cccccgatgt gacggggccg
     3301 ccgcagtccg ggcagtgcgc caaagccaac tcccgtaagt gcgcggcggc ttgccggttc
     3361 ccccggtagg ccgcgtacgc gacatggaag gccccctgct cgggctggcc gagcgcgcgc
     3421 agcgccaccc cgagggcgaa ccggccgctg gcgtgctcgg agtcgaggta cacgcacagc
     3481 gccagcggat acagggcgcc cgcggtgtcg ccgctgttcc acctgccggc gcccagcatg
     3541 aactggcagc ccaccgaggt cggggccaac tccgcggcgg cggcgagcag tcgtacggcg
     3601 tcctggtacc gcccgcgcag gaagaggtcc ctgccctgct cgaacagcgc cttcgcgcgg
     3661 gccagttcgg agctcaccgg ccaccccgcg cccgccccgc cctggtctgg aggcggctca
     3721 cacagaccca gcacgccagc cagccctgct ggtccgggcg gggcgggctc agcggcggag
     3781 tctccaggac accggacgcc aggtacgcct ccgccgtccc ggccccgatc cggccgacca
     3841 gctcgccttg gtagatcttc ttcgcgcaca ggtcgcacgt gtcgccgacc gtcggctgct
     3901 ggacgctggc cggccagcga ccgacgacgt actcccacgc ggcggcggcg aagggcgcga
     3961 agcccgcggg atcgaccgcg cccgagctct cggggacgct gccgcgtcgc gcgtgctcgc
     4021 gggcggcggc gcggtcgaag acgaaccact cgctgcactc ctcgcagacc agccagggtg
     4081 tggcgctgct cgcggactgg ctgatcagcc ggtcgaaggc gccggcctgg gcgcgctcgt
     4141 cccagttgag ggcgcgcacc aggccgaccg ccgtccggaa gaagctgcgc cagtacgcct
     4201 ccgacaccac cacgtgcttg gtcggcaggt gatagccgct ggaccgccgg gtcgtcgtgc
     4261 cacaggtgtc gcacaccgct tgctcacgca tccgtgcccc cgtcttcgtt ggcgtcgtgg
     4321 tcaggtgttc accgggctcc aggtgccggc gtcggcgtcc cagtgcagtt ccgcgccgcg
     4381 tacgccgtcc gcgccgaggt cgaggtcgat ggcggcgagg cgcgcggcgt cgtcgaagtc
     4441 gtccgggagg accagcgtcg tacgctcgcc gggcgtgagg acgtccaggt cccggcggcc
     4501 gctgcggcgg tgccacagca gccgcagccg gcccgtggcg gtgaccgtcg actcgccgag
     4561 ccgctcgccg agcttgccgg cgaagctctc cacgaacggc ccgaggaatt tcaccgcgac
     4621 gaagccgcag gcgagttcga cgacggggac gccggtgagc ctggcggggg cgtcgtccgg
     4681 gcgtccgccc gtggcgtagg aggcgtcgga ccgcgcggcg gcggggctgg cgaagaagcc
     4741 ggtctcgtcc ccgccctcca gcagcacggc ccgccaccgt tccccgagcg cgtcgagctc
     4801 ggcgaggttg tggccgagcg cgccgaacgc cgcctcctcg gccggccggt cgccgacctc
     4861 ggcggccagg cccgcggcgc ggcggcacag gtaccggccc tcgctcgccc ggccggcccg
     4921 ggtcagcgcc gctcccaggt cgcacagcgc ccgtgcctcg gccctgcggt caccggccgc
     4981 gcgggcccgg gcgagggcgc cggaggcgag atcggcggtg gtgtcggcgc ctgcgggcgg
     5041 gacatgtgcg gtcatacgat ctcttctcgt ttctctcgcg ggcggggccg cgcctggtgc
     5101 gtaccggcgc tgacgtggag ggcggcgggc gggaacaccg gttccagtga gtaggcgccg
     5161 gcgagctccg tgagggtgtc cagggtgggg aggccggtcc actcccactc gcgggcggcg
     5221 cacgtgcggc aggcggtcgc cctcgaccca gatctccgcc tggagcctgg cttcccaggg
     5281 cgcgcacggc gtgagcctga cgacctcctc gacgctcagc acccgcagcg cggtggggcg
     5341 cgggcgcgtc gccgtggggc gggtggtgat gccgcgttcg gtacgccagg cgcgcagttg
     5401 ggcggcggcg tcgcggtatg cctccatctc caggaggtgt tccaggctct ccagcggggt
     5461 ggaggcgagc gcgccggtcg cggtgtcgta cccggtgacg acggtcgtcc cgccgatgct
     5521 ccccaatccg ccgaggtcgc cgccgcggcg ggtgatgttg ctggccatga agttcacggc
     5581 ccgctgctgg gcgagcgcgc cggccttgcg ccacaccgcg attcccgggt tcagcccctg
     5641 tgcgttcagc cgatggacgt tccagctcgc gggcgcgccg ccgtcccggc ccatgatgcc
     5701 ggcgatctgt cgggtggccc gctcggcgtc cggggccagc accaggacct ccaccgtctt
     5761 gtccggctcc atccggacca cgatgatccc gtcccgcgcg gtcccgtccc gccgctcggg
     5821 cgctcccagt ccgtaccgct ccatgagcgc ggccgccagc gccgccgcca actcgcgctc
     5881 cgcgcgcggc gtacggggat gctcggcgag tgcccgcagc gactggagcc ggcgggagat
     5941 cgacggggcg tccttcggct cgctcggtcc acccggcgcg accacgccac atcacccccc
     6001 agagggacac cgctgcctcg tcggtcgaat tgaaccagac ctcgccctcg cacctcagcc
     6061 gaacgggcga actccgtgcc cgtcgccgcc gcgtgcggct gaaactcccg cggagccggt
     6121 tctgacctgg gcttttacct gggctctcag ctggctgcct gccgcgctgg cgcgcggtga
     6181 actcacctgc ggtggccggg atgccggcct gctcgcagaa gcccgcgagc ttgggctcgc
     6241 actgcctccc gggcaggcgc atgagggtcc aggcaccgtg gtcctcggcg ccgatcacgg
     6301 cgacatcacc gcagcgcagc tgctccacaa gacaagcgaa caccattgcc tctgccgcga
     6361 aatggcggcg gaccagcatc tccaggtgca ccagcgagcg ctctccaccg agtccgtcat
     6421 gcggaccgag cggcgtggac ggatacgccc caagagcctg cacggtccgg cagttgcgct
     6481 cctctgcggc tgaccgaccg cggtgccggg ccggcagctt gcgggcccgg caccgccagg
     6541 tgtctacggg atttcgacac cggcaaagcc agtggacccg ggttccggcc ccatcccgtc
     6601 gggatcgtcg tcggggtcct cgaagcgtcg cacggcctca gcgacctcgt ggtcgctgga
     6661 ctcgacgcag aattcgttgc cctcagggtc ggccagcacc accacctcca ggccgcaact
     6721 gcggttcgtc cagcgcctct cgacgacccg agctcccagg ccggtgagcc ggtcgacctc
     6781 tgcgcgcagc gctccttggg cggggttcag ccacaggcgc gtccggcttc gcggccgccc
     6841 tggtgctcgt tcctccacca ggcccctttg atgcggccgt gagattcatt actacctatg
     6901 ggttgctcaa cctgacccac ccaagtgacc ccgagagcaa gttgataacc aatccgaccg
     6961 gcggctatga gggtgatttc ggagaaatcg ccgacctgtc acgagagttg gggaagcgtc
     7021 ttttccggat cgggtacgac ctccctgacg ccgggatctt tgtcgtggcc gaggacggcg
     7081 agttctactg catccatcat gcggaggcgc attatctcgg ctctgacgaa ttcgagttct
     7141 tcggcaactg ggtgaagggt gacatgcaga gtgtctgagc gctgggaagg ggcgtcaatt
     7201 cctggcgcgg catcttctct ctccgggtcc cgcacgtcgg tcggacctgg tggccgaggc
     7261 cgccggtacc aaaccttccg acgtggaaac cgctcgactc gctctggatg cactgggctt
     7321 ccccattgtc tgctgggcct ggggcgacct tctcaccacc ggtcatcccg gacaccgcat
     7381 ccgctcaaca tgatgagagg gggcgccgca cccggggacg agcagatgca gtcagtcgcc
     7441 tgcaggttgc cgccgctcgc cgacgcgtcg tggggagagc ggtatgagcc aaggcggccc
     7501 tcgttgaaga gccgcccacc cccgagccgt tgccgtggcg ccggaggtgg gtaccttcgc
     7561 gatgtcgtgg cttgaccggg gaggttggtg gaagcgtggc acggggcact gacggctgca
     7621 cgccctatcg ggggctgatc ttggacttcg taggggtgct gaccgagggg gtacaggccg
     7681 cgctgcactc ctggtgcgca tctgagggcc tcgcgcccga tgcctggcga atcacccttg
     7741 ctgacctccc cgaggggcgg gaactgtacg cggcacttga gtcaggacgg ctgtcgcaag
     7801 ccgagtggaa tcgccgcacg gctccgctgc tgggggtgga ggaccaccac gatctgatgg
     7861 gccgggcgtg gtcgggtgtg cgcccagcgg tgaacatgat cgccttggcg cgggctgctc
     7921 ggcgagccgg ttacacggtg gctctgttgt cgaactcctt cggcctggac ccgtacgacc
     7981 catacgaggc gttgggcgtg tggaagctgt tcgacgtgac ggtgatctct gagcgggagg
     8041 gcatcgcgaa acccgatccg gaaatctacc aacgcaccct ggaacgtatg gatttgcccg
     8101 gggaggcgtg tgtgtttgtc gatgacaacc cagggaatct gcctccggca gccgcgctcg
     8161 gtatcaccac ggtccacgcg gacggccaag ccgacacggt caagcggctg tcgagtctgc
     8221 tggggattct cccggccgcc ggcagagttc acaggtagtg ccgccgctct cgccgcgctg
     8281 tggtgtcgtg gcccaggacg ggcgccagac cgcatcgggg agcctgggaa gggtgccaag
     8341 gtgggtgatg attccgcgga gttccggatg cggattcgtt cgggggagga tgagcgagag
     8401 cgggtaggcg agggtcggat tcacgatagg gatgcggcgc aggtcgtggt tcgtcggcca
     8461 aatgtaccgg tcgcgtgccc cgaccagggt ggccacgtcc gcggagtctg cgagggtgtc
     8521 caggagcact tcgtctccga agtgcgggcc cgtcgcgtcg atgcgcaggt cgaagtccgt
     8581 ggcgagctga tcgtagaact ccgcccattc gcttcggggt gcgatacccg gcacccatat
     8641 ccggtgtctg cgcagctgtg gcggcgtcag ccttcgcgcg gaggcgagcg gatgcctcgg
     8701 gccgacgagg agttccagcg gggagtcgaa ggcgtggatc atctgcaggg cgggtggtag
     8761 tacggccggg gcgatgacgg tacgaaacga ggcgtcgata tcgcctgctt cgacggcggc
     8821 gaccaccacg cgtgggtcgt tggctctgag ggtcaccacg tcgagattgg cttcggggcg
     8881 cgaccgccaa taatcgtgca ggacgacggc ttgcgcgctt cgcaggccga gaacgtcgat
     8941 ccgaagggcc cgggagccgg gcctgaccgc gttgatggcg cgctcgaccc ccgcgacgat
     9001 gctccgggca tgggagagaa acgcctgacc gtcgagtgtc agctcaaccc ctcgggcggt
     9061 acgtgtgaac aggcggactt ccagctcgcg ctccagggtg gcgatccgct tggagactgc
     9121 ctgctgtgtc acgcccagct catcggccgc atgctggaac tggccgagct cggctgcccg
     9181 gacgaacgat cgcaatgcct cggtgtccac tgcttcacct taggcgtgct caacccatcg
     9241 ttgtggctgg ccgaggatcg gttgtccgaa cgagctccgc ctgggcgttg ttccagttgg
     9301 tgagccggcg atggctgatg agggctgagg cgaggccgac gaaggccggg aagtgctcgg
     9361 cgtcacccga cgcctcaaga cggtgatcgc cttgccgtag ccgtagccgt agccgtagcc
     9421 gtagccgtag ccgtagccgt agccgtagcc gtagccgtag ccgtggtcgg tgggtgtgtc
     9481 ttcggtgggg tgtgactggc gccggtttcg tgggtgaggg gtggggcggg tccttggggt
     9541 gttgatagtg caacccgtgt gaagtcagtg gttgttgggg caaaagtggg aggaggtatt
     9601 ccgggatatt cataccagaa cggtcgattg tggatcttga atcgcggtga gccgcgtggt
     9661 tctctgtcga tcacacccag ggcggcgacc ggggcgcaag ccccgtcgat cggcggcgag
     9721 ttgggagtca tcccggcgtc cgcggaacct tccgcaccgg aacgcgttcc gccctgggcc
     9781 gtcgcaccag tcaacgggga ggtgaactgt gatgcagggc aacgaactcc aggagaccga
     9841 caagctccag acctacgagg cccccaagat gatcgagtgt ggctccttcc aggaggacac
     9901 cggctacttc gggctgaccg gctacgagaa ccttttccac ttctacgaca agctccacta
     9961 gggccgacgc tgatgcggca caccccgtaa ggacactgag atggccgatg ccggttggca
    10021 gtacttcgtc gcgctgccgg atgacgaggg cgcggcggcc gccatcggcc ggttcccgac
    10081 ggacggcgtg cgtgtcctgt tgaggcaccg gtccggccgg ccctgtgtgg ccggccggct
    10141 ggccgaggac cgggtcctgg tggtcgaacg gggcgacacc cggctcgccg tgatcggcca
    10201 ctgctcggcg acccgggacg aactcgccac ggcggccgac cggatcgacg atctgaccga
    10261 cctggaccga ctgggcctgg cctgggccgg cagctaccac ctcgtcgcct ccgtgcgcgg
    10321 ccgcctgcgc gtacagggca ccgcctccgg gctgcgccgg atcttccacg gtgagctcgg
    10381 cacactcacc atcgccgccg accgcgcgga cgtcctggcg accgtactgg acgccccgct
    10441 cgatccggcc ctgctcgccc tgcgcacgct ggacgcgctg ccgcacccgc tgggcgacct
    10501 gccgccgtgg cgccatgtct ccgcagtccc tccgggctgg tacctggcac tgccgcagga
    10561 cggccggcgg ccactggtcg aacgctggtg gcaggccccc gaaccggtgc gttcgctgac
    10621 cgcgggcggc cagttgttgc gccaggcact ggaggacgcg gtgcgggtgc gcgcccgcgg
    10681 cgcccggccg atcagcgcgg acctctccgg cggcatggac tccaccagca tcgccctgtt
    10741 ggccgtccgg caccggccct cgctcaccgt gctcaccatg gagaacgacg atcgggccga
    10801 cgaggacgcc cggtgggcca ggcaggccgc cgccggcctc ccggacctca accacatcgt
    10861 ctactccagc cgcgaactgc cgcggttctt cggcggcctc acggcggtgg agggcgttcc
    10921 cgacgagccg tccaccgccg tactgagcgc gccccgactg cgcgccgtcc ggcagcgggc
    10981 cctcgcgcac ggctcgcggc tgcacctcga cggactcggt ggggaccaac tgctgtgcgg
    11041 tcacccggcc tatcaccacg acctgttgcg gcaccggccg ctgttggccg cccagcgcct
    11101 cagggtgctg acccaactac gccggagcaa ctggggcacc gaggcccgag gtctgctgga
    11161 gggccactcc taccgccgct ggttcacgga ccgggcccac ggccgcgccc ttcgcacccc
    11221 gagcccgctg tccgcctggg gccacttccc gggcctgccc gcatggctca ccccggacgc
    11281 cagggacctg gtgacccgca ccctgcgcga cacggccgca accgtcgagc cactcgcgtc
    11341 gacgcgaggg cggcacaacg acctcctggc catccaggac gccggacgga tggtccgcca
    11401 gtgccaccaa ctgaccgaga ccggggactt cagccatacc tcccccttcc tcgacgaccg
    11461 cgtcatccag gcatgcctgg ccgttcgacc agaacaacgt gtcactccct gggagttcaa
    11521 acccctcatc aaggcggcga tggcggacgt catgccgccg gaggtactga cccggcggac
    11581 caaaggtgac ggcaccacca tcgtcgccga gggcttcgaa gcgcaccgtc gggaactcgc
    11641 ggaactctgg gacgattccc gcctggccgc cctgggcctg gtcgaccccg gaccgctgcg
    11701 cgagctgttc gacctgccct acaccaacag cctccaccaa ggtgcgatgc agtccgcgct
    11761 cagctgcgaa ctatgggccc gggcaacggc cttccaaccg gcagccgcca cccgcccctg
    11821 agcccgccac aggacccgag caggagacaa ccatgacatg gagactgcac cccgacgtct
    11881 cgaccgtgga caccgactac ggaaccgttc tgctgaacgg cagcgacggc cgctactggc
    11941 agctcaacgc gaccgcctcc ctcgccttcc gtgccctggt cgagggcagc acggtgcagg
    12001 aggccaccga gcggctgatg gcctccttcg acgtcgacga gccgcgtgcc cgcgcggacg
    12061 tggagcagct gatggccagt ctccggcagg cgaaggtggt gcagcaggcg tgagctcggt
    12121 cgtaccgcac cgcacggagg tccggctctc gccgggccgc cgccttctgg tgctgctcgc
    12181 cgttgccgtc gcccggcgcc tggccgccaa gccgccggcc cggacccgcg ccatactgac
    12241 ccgcctgcgc aggggcgccc gcgcggccac gtacgcggag gcccgcgccg cccgggacgc
    12301 cgtggtggcc gtcagccttc gctgcgccgg ggacgagggg tgcctgcctc gctcgctggc
    12361 gaccgtcctg ctctgccggg cgcgcggcac ctggcccacc tggacggtgg gagtacgggc
    12421 ccgtctaccg ttcggcgccc atgcgtgggt cgaggccgag ggacgcatgg tcgaggagaa
    12481 ggcggaccct tcctacttcc gacccctgtt ctcggtgccg gcggaagggg cctgacccgg
    12541 gtctctcaac cgtcggccgg gccgccgtca gggaacgaac agtgcgaacg aacagggaag
    12601 gcccggtgga aaccaccggg ccttcccatg tgagtagcgg ggacaggatt tgaacctgcg
    12661 acctctgggt tatgagccca gcgagctacc gagctgctcc accccgcgtc gacaacagca
    12721 actctacggc acttccccgg atcggccgaa tcgacctccg ccccggcccg acgccgggct
    12781 tttcgggcca ccgcatcgac ctcggagccg agcgctcctt gacggagcgc gtccgcagcg
    12841 ccgcggcgga gaacgcggta tccgcccgcc ggggcgccgc tgtgcgatcc cctccccgtg
    12901 tcgcctccgg ttttgctgcc tctactctgg ggcgccaggg acgaacggcg aaaagcgatg
    12961 agcggcgaag gaacggagcg tgcggtgacg gccgggggag cgagcggcga agagctgata
    13021 ggcaaggtcc tcgggggccg ataccgggtg accgcgacga tcgggcgggg cggcatgggc
    13081 gtggtcgccc gggccgtgga ccaactgctg aaccgcgagg tcgccgtcaa ggtactgcgg
    13141 gccttcaccg acgcctccgc acccgaactg gccgatctgc ggacccggat gcagcgggag
    13201 gcgcaggccg ccgcccgtat tcggcacagt ggcgtggtca ccgtgcacga cgtgaccgag
    13261 gagcagggcc tgccggtcat cgtcatggaa ctcgtcgacg gaccttcgct cgacgacgtg
    13321 ctgacggagc gcggcacgct ggagccgcac gaggcggcgg cgatcggcgc caagctgatg
    13381 gacgcgctcg acgccgcgca ccgggtgggc gtactgcacc gggacgtcaa gccggcgaac
    13441 gtgctgctgg agcacggcgg gcgggtcgtg ctcaccgact tcggcatcgc ctccatggat
    13501 gcctccggcg acgaggccat ggccaagctg acccacagcg gtcagctggt cggctccctg
    13561 gactacctgc cgccggagcg cgcgcggggc acggtgccca gccccgcgtc ggacatctgg
    13621 tcgctcggca tgacgctgta cgcggcggtg gaaggcacgt caccgttccg ccggacgtcc
    13681 gtgtggtcca ccttgtcggc gatcgtcacc gagccgctgc cggagccccg gcgcgcgggg
    13741 ccgctgacgc cggtgctgca ggcactgatg gcgaaggagc cgaagaaccg gcccacggcc
    13801 gagcaggcgc gtgaaatgct gcagtgggcg gccgcgggca ggacgatgaa cctcgcgccg
    13861 ccggctccca tggcccccgc ccctccgccg gtacaggccg ttggcccgac gcctccccag
    13921 gcagccaccc cggcgccgcc gcccggattc ggtgccgtgc cgccgctcca cccgtcgtcc
    13981 ccgcagcctg cgcacaccgg ttacccgccg tccgtcccgc cgcccgtcac ccagccctgc
    14041 atgccgaccg cgggccacgc tcacggtgcc ggccccgccg caggccgcgg ccggcgctca
    14101 cgccgtaccc tcatcgcggc cgcggccgcc ttcgtcgtgc tcgcgggagg cggtgtcacg
    14161 tacgccttga ccgcgcagcc cggtgcggcg gacggcaacg ggccgcaggc agcgcccgga
    14221 gccagttccc cggtgggcag cggccagccg agcggcggcg gaacagccga cggcatcaac
    14281 tccccgggct cctcgccgtc cacgggatcc ggaaacccgt ccggcgcgac cactccctcc
    14341 tccgccccgc cgtcgaccaa gcccggtgcc acgccgagca cgtccgcttc cccttcggcc
    14401 aagcccagtt cggggcccac gacgtgcagc ggctggagcc accacgaccc gaagccggga
    14461 acgtacggct acctgtccgg cgatcaccac atcctgacgg ggccctacca ggtctgctcc
    14521 tctgtggccg cgaccacgtc aggcacgaag ctgtggttcc actgctcggt cgagaacgcc
    14581 tacggccaca agtggctcta cgtccgcatc ggcgggacga agaacgcggg ctggatgtcc
    14641 gccgacaacg tcatccggca gagcggctgg ccagtccgtt gttagcggcg ctagcggcgt
    14701 gatcgaagcc gtccggggcc ggatcgtccc gaggggggaa gctcacagcg ctacggcatg
    14761 ccgttcacag gggctggaag cggcagctgc ccggatgttc aacgtagacc tgtcccgtct
    14821 cgacggcgta gaaccagccg tgcaggcgta gcttccccgc cgcgatcctc gtggcggcga
    14881 acgggtacga gcgtatgttg cccagctgta tcagcgtgtg gagctgggca acggtgtcgg
    14941 ggtccggacg gtcggaatgt gtcacgcgtg ccgtgcgtgc ggtcggcggt tcgcctgcgg
    15001 cacgtgtccc gtggccggag aggctgagcc acttctccag cagcggcagg aacgacagtt
    15061 gacgcgggtg catcaggccc tgcacggcgg cgcagtgtga gtggctgcag acgatgatgt
    15121 cgggtacgtg cagggcggtg accgcgtact caagtgtcgc ggcgacgcca caggccgcct
    15181 ggggccggta gcgagggacg atgttgcccg ctacccgtaa ttcgaacagt tcgccggggc
    15241 gggctccggt gaacatggtg gggaccacgc gggagtccgc gcacgtgatg aacagcgcca
    15301 tcggccgttg gccatgagcc aacttgccca agttgccgcc ctgttgggtg acgcgttggg
    15361 gaaagctacg ggcgtggtcc aggaacgctt gcacatcgac ctcgcttctg gtggacggtc
    15421 ccgcgaagag cgggctcggc gggccgccca tggcggcagc cgcggccgtg ggtgcgcggg
    15481 caggccccgc gcagggggtg gctcggcccc gggtcggtat ggacgcgcgt cggtctgccg
    15541 ccgacggatc acgccacgac gtgggtctgc ggccgtcccg gtctgggcac aggacggatc
    15601 gcggcacgcc ccctcgggag cgagggggtg acatcggtct gccgggtgtg cgggtgggac
    15661 tgccgcccac ggctgctgcg cttcaggaaa ttgcgcacct cgcacccgcc cttccagcaa
    15721 cagccgcacc gcggggaggg ggaggagatc ggtgaggtcg atcccttgac cgggcctcag
    15781 cccgccctct ccccagcggt tcgcaaggaa cactttcagc cagtctgacg ccgggtgatc
    15841 agggtgtgaa ccaaggaaat ggcaatggcg caccgagagt tcgttcagga ccttccaggc
    15901 aatccccaag aacgcaatcc cggtgtgaag cgacctgccc cgcgcaacgt ggtcacttcg
    15961 gtctacggcg tgtactcgac cgtcggcaga tccggctcga tcagcttcag gacctgttgc
    16021 acggcggcat cggggccgtc cgcgcggtgc acggcggcga ccgtgctctc caggtagggg
    16081 gaatcgccgt gcaggggacg gtagaccacg cccgggatgc ccaacgtgcg cacctgctcg
    16141 ggggcgagcg ccaggcacag cccggcggcg acgtagctga gcaaccccgg caggtcgtcc
    16201 gcctggccgc gtggtcgggg cgtgaagccg gcctgcgcgc aggcgagcgc aacctgctcg
    16261 gccagtccgg gcagggcgcc gggcttgggc accgcgaact gctcctcggc gagctcccgc
    16321 aggtcgaggc gtgacgtggc gaccagcgga tggtgttcgg gaaggaccgc catcagccgt
    16381 tccacgccga tccgccggac ggtcagtcgt tccgtgccgg tgacggggag ccggaccagt
    16441 gcgaggtcga gcgtcccggc ctggagcatg tcgaccagtt ccgcggaggg gtgctggccc
    16501 aggtgcagcc gcagatcggg caactgcgcg tgggtgcggc gcagtaggcg cggcaggaag
    16561 tcgtagccga ggctgctgat gaacccgagc cggagctcgc cctcgtgtcc gctggcgatc
    16621 cgccgggccc ggtccacggc ggcggcctgt gcttcgagca gccgccgggc gtcctcggcg
    16681 aacgcgcggc cggccggggt gagtcgggct ccccgcggca ggcgctcgaa cagggcgacg
    16741 ccgaggccgc gttccagtcg ctggatcgcc tggctgagtg gtggctgccg catggcgagc
    16801 cgctcggcag cgcggccgaa gtgctgttcc tcggccaaca cgacgatctg ttggaggatg
    16861 cgctgggtca attccatatc gcacagcata tgtaagccgc agggaagaag tattggacga
    16921 tatgggtcac cgaggatttg actggctgtc aacggtccgg cgcgcgaagc cggccccggc
    16981 ggagatcgga gacatgccat gtcgacagga cgggaaacgg acggtggcgt cggtccactg
    17041 gacggcgtgc gggtggtcga gctggccagc gtgatcatgg gcccgtacgc ggcccaacag
    17101 ctcggcgacc tgggggcgga cgtgatcaag gtcgaaccgc cgaccggtga cctgacccgc
    17161 cactacccgc cgacccgcaa ccccgggatg ggggtgatga ccctcaacct caaccgcaac
    17221 aagcgcagcg tcgccctcga cctcaaggcc ccgggcggcc gcgacgccct gctcgccctc
    17281 ctcgcgagcg cggacgtctt cctcaccaac gtccgcccgc aggccctgcg gcgcctcgac
    17341 ctggcctacg aggacgtggc ggccgtcaac tccggcctgg tctacctcaa cgcccagggc
    17401 ttccgcagcg attcccccct cggcgggcac gcggcctacg acgacgtcgt gcaggcggcc
    17461 tccggcctgg tgtggctgaa cgaacaggtc agcggcgtcc cgcactacgt ccccaccgtg
    17521 ctggccgaca agatctgcgg cctggtgatc gtgcagtcgg tgctcgccgc gctccaccat
    17581 cgtgaccgca ccggccaagg ccagcacgtc gaggtcccga tggccgacac catgctggcc
    17641 ttcaacctcg tcgagcacct ggcagcggcg accctcagcc cgcccgaggg gccgatcggc
    17701 tacccgcggg cgctcagccc ccgccgcacg gcctcccgca cggcggacgg ctggatgtgc
    17761 atcctgccct acagcgaccg caactggcgc gacttcttcg ccttcgtggg ccgcccggaa
    17821 ctggccgacg acccgcgctt cgcgtcgtac gccgcgcgcg tccacaacgc cgacgaactc
    17881 tacgtactgg tcggtgagtt cacgatccgg cacaccaacg cgcaatggca ggagttctgc
    17941 gacgcgacca gcatccccgc cgccccggtg ctgagcctga cggaggccgc caccagcgcc
    18001 tacgccgccg aaggcggtct gctccgggag gccgaacacc ccaccgaggg cggctaccgc
    18061 ctcatcggcc agcccgtgcg cttctgggcc acgccggccg gcctgcaccg gcactgcccg
    18121 cgggtcggcg agcacaccga ggaggtcttc gccgagatcg gccaccggcc gccttcctga
    18181 gccgtcacca gtcaccagca gtcaccaaca cccgtacccg cggaccgaat cgaggccccg
    18241 tgaacgagca agcacccctg gacgagcagg cagggccgaa cgcgtgggaa ccgctgcgcg
    18301 tcgagcaccg gccgaacggc gtcaccgtgc tcaccttcga caaccccgac aagcgcaatc
    18361 cgatgaccgg acggctgacc gcacagtggc ggcgcgccat gcgctccctg cgccaggacc
    18421 ccgacctgcg cgccctcgtg atcaccggca ccggccccgc gttctgctcg ggcgccgacc
    18481 tgggggaact cgccgaccga cacggccagc ccctgtccgg cacccgggcc cagctgtcgg
    18541 acttctaccg gacctggctg accgtccgtg acctgtccgt ccccaccgtg gcggccgtca
    18601 acggccacgc ggtgggcgcc ggactggcgc tggccctggc ctgcgacctg cggtacgccg
    18661 cggcggaggc gaagctcggc gcgccgttcg tccgactcgg cctgcacccg ggcatggcca
    18721 ccaccgccct gctcgccgag gccgtcggcc cgtcgagggc gcgggaactg ctgctgaccg
    18781 ggcggctgat cagcggagca cgagccgcca ccatcggcat ggtgcacgag gcgctgccgg
    18841 ccgaccaggt gcttcgggcc gcgctcgacg tggccgacga gatcgccgcc gcggccccgc
    18901 tggcggtccg gctgaccaag agcggcctgg cccaggggca gcccaccgtc gagcgggcgc
    18961 tgacctggga gggactcgcc cagtcggtga cgaccaccac cgacgacttc cgcgagggcg
    19021 tccaggcccg ccgggagcgc cgccgcccgc acttcacggg cgcctgacca ccaccgccga
    19081 cggtgtaccc gtcaccccgc acacccacca ctgcgtatac ccgtcactcc atacacccac
    19141 cattccgtcc gaggagtacc cgatgaccct ctccgacctc gatctcgggg ccgtccaact
    19201 tccgcccgaa gcaacccggt tacggcagga ggtgcgcgac ttcctcgccg cggaacgggc
    19261 cgccggcacc gtcctcggtc ggcccgactc ctggctgacc ggctgggacc ccgccttcac
    19321 ccgccgcctg gcccggcgcg gctgggtcgg catgaccctg ccgcgggcgt acggcggagg
    19381 cgggcgcagc aacctcgaac gctacgtcgt catcgaggag ttgctggccg ccggcgcccc
    19441 ggtctcggcc cactgggtcg ccgaccggca ggccgggccc accatcctgc gccacggcac
    19501 cgaggcgcag cggaaccact tcctgccccg gatcgccgcc ggcgcgtgct tcttctcgat
    19561 cgggatgagc gagcaggaca gcgggtcgga cctggcctcg gtacggaccc gggcggagcg
    19621 gaccgccaac ggctggcggt tgaccggcac caagatgtgg acgggcggcg cgcacgtcaa
    19681 cgaccacgcg atcgtgctcg cccggaccga cgagtccgag gaccgccacg ccgggctgag
    19741 ccagttcatc gtcgacctgc gcgatccgca cgtccgggtc gagccgatcc gtctcctcag
    19801 cggcgagcac accttcaact actgccacct ggaaggcgtc gagctcaccg acgaggcact
    19861 gctcgggcgc cccggtgacg gctggcggca ggtcaccggc gaactggccc tggaacgcag
    19921 cggacccgag cgcttcctgt ccacgctgcc gctcctttcc gccctggtgg gacagttgca
    19981 gcggaccggc gcgtgcagtc ccgagcagca ggcccgggtg ggccggctca ccgcccgcct
    20041 ggcgtccatc cggcagctgt cgttgggggt ggccgcggcg ctggcggccg gccgccccgc
    20101 cgacgtgcag gcggccctgg tcaaggacct cgggacccgc ttcgagggcg aactcgtcaa
    20161 cgaggtccgc gagatcctgc cgatcgccgc ccgcttcgac gccacccgcg gcagctaccc
    20221 cgaactgctg gccaccgcca tcctgcatgc gccgggatac acgctgcgcg gcggcaccaa
    20281 cgaaatcctg cgcggcatca tcacccgagg tctggagctg tcccgatgag catcgaaccg
    20341 tgcgccgagt ccgtcgccga acccctctcc gaacgcgccg tcgacgccct gccgccgctc
    20401 gacacggctc cgaccgccct cgtggccctg gcgcgggaag ccggctaccg cagcaccccg
    20461 gcaccgctcg ccgagacgct gctcgtcgca cgcccgctgc tggccgcggc cgggctcgcc
    20521 gtcccggacg gccaactcac cgtggctgcc ggcccgctcg acgtgcggtt cacccacgtg
    20581 gacaccgaca gcagggtcgg gccgtcaggg gcggcaggca ccgatgacgc cctacgggtc
    20641 agcggcacgc tgcaccgggt gccgtgggcc cgggccgccg aagccgcggt ggtgctgacc
    20701 gagggcccgg gcggcgggcc cgtgctcttc ctcctcgaag ccaccgagtg gcggcaggtg
    20761 gcgggcacca acctggcggg cgagcccagg gacgacctgg tgctgctcga cgtggccgtc
    20821 cccgccgagc gggtccgccg cgttccggcc gcgctggtcc gggaggctac atggcgttcc
    20881 ggcctggcgc gggccgccct gatcgccggt gcggcacgcc gctgtgtcga gttgacggtc
    20941 gatcacacct cctcccgtgt gcagttcggc cgaccgctga gccacttcca ggccgtcaag
    21001 caggaggagg ccaagctcgt cgaggaggcg gccctggtcg ccgcggccgt cgaggcggcc
    21061 gcgtccgccc tgtccgagcc ggactccgcg gagttcaccg cgtggctcgc cgccgcacag
    21121 gccgccgcct cggtggccga gatcacccgc atcgcccacc aactgcacgg cgcgatcggt
    21181 ttcaccgaac tcagcccgct ccgcctggcc accacccgca tgtggtcctg gcgcgacgag
    21241 gacagtggtg aacgggcctg gcaacaaagg atcggtcggc acgtactcga cggcggcagc
    21301 ggcgggttgt ggcagcaggt ggccggcggc tgacccgagg ccgagtcagg ccagcacctc
    21361 ccgaagccag gacctttccg cgcggctggt ggcgcgggcg atggtgagca tgccgcgccg
    21421 gtaggggtcg tcggtgtcgt cgctgcgcag ggggcggtcg ccgtcgtgga agaagctcgc
    21481 gggctgttcc aggaactcca gtcggcggcg cagtaccgcg tgctggtcgg tggtgtcggg
    21541 cagttgggag aggaaggcca ggaccgtgaa ataccgggtg ctgtcggtga tgtcgaggcc
    21601 gttggcatcc cgcagtcggc gcaccagctc gcggcggccg gcgtcggtca ggctcagggt
    21661 gtggcgctgg gcggccgagg tgcccggttc ggtccgtcgg tcgagcagcc cggcacggac
    21721 cagccgattg atcgccgggt agaggctgcc gtcgctgacc ggccgggcat ggccggacaa
    21781 gtgcgcgatc cggtggcgca gttgatagcc gtgcagcggc ccctcggcca ggaatcccag
    21841 gatggcgagt tcgagcatgc tgctactctc gcacatcgaa acgaggtacc tcgaatcgat
    21901 ataggggcag ggatgacgta caccgaggaa tgggtgaccg cgcgggcgcg ccacgcctac
    21961 gagcacggcc aggcatcgtt cgacatacga cccgaacgga cggcgctgct ggtcatcgac
    22021 atgcaggacg aattcgtccg gcccggctgg agcccctact gggtgcccgc ggcgacccgg
    22081 atggcgcccc ggctgcggca actggtcgaa gactgccggg acgcctcggt cccggtgatc
    22141 tggacgatct tcgacgacac ccatctcgga ctggaccggc cacacgccct gcgcttcctc
    22201 ccgcacgccg acaccgactg gcgccgaccg ggccccgccg aggtctgggg ccggatgggg
    22261 cgccgccccg acgaggtcgt gatccgcaag ccgtcgtatg gggcgttcta cgacaccccg
    22321 ctcgacaccg tgctgcgcaa cctcggccgc gacacggtca tcgtcacggg aacgctcacc
    22381 aactactgct gcggcaccac ggctcggcag gcgtatgaac gcggctacca ggtcgtcttc
    22441 ggatccgatg tcacggccac cgacgacgag tcccggcagg aacccgaact cgccgttctg
    22501 cgcaagggct tcgcgcgcgt cctgaccgcc gcgga
//
