LOCUS       NZ_CP054019            11297 bp    DNA     linear   CON 28-JAN-2024
DEFINITION  Streptomyces sp. A1-5 chromosome.
ACCESSION   NZ_CP054019
VERSION     NZ_CP054019.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. A1-5
  ORGANISM  Streptomyces sp. A1-5
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 9459690)
  AUTHORS   Dashti,Y., Sumang,F.A.M., de los Santos,E.L.C., Alcantara,E.P. and
            Challis,G.L.
  TITLE     Structural revision and biosynthesis of polyketides FXJ15321 and
            FXJ15322 from Streptomyces sp. A1-5
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9459690)
  AUTHORS   de los Santos,E.L.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2020) Warwick Integrative Synthetic Biology
            Centre, University of Warwick, Gibbet Hill Road, Coventry CV4 7AL,
            United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: JUN-2019
            Assembly Method                   :: Flye v. 2.4.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Oxford Nanopore MiniION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/28/2024 04:28:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,328
            CDSs (total)                      :: 8,230
            Genes (coding)                    :: 7,847
            CDSs (with protein)               :: 7,847
            Genes (RNA)                       :: 98
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 156 of 383
            Pseudo Genes (incomplete)         :: 280 of 383
            Pseudo Genes (internal stop)      :: 26 of 383
            Pseudo Genes (multiple problems)  :: 73 of 383
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 22:08:01
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 7000103
            Orig. end                         :: 7011400
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP054019.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Philippine National
            Collection of Microorganisms.
FEATURES             Location/Qualifiers
     region          1..11297
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="24"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11297
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11297
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6297](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6297)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            419..1156
                     /gene="hemQ"
                     /locus_tag="HRD51_RS30765"
                     /old_locus_tag="HRD51_30385"
     CDS             419..1156
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018536947.1"
                     /locus_tag="HRD51_RS30765"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HRD51_30385"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_205367271.1"
                     /transl_table=11
                     /translation="MDDMTDASTPASDKIPNAGKKAKDLNEVIRYTLWSVFKLRDVLPE
                     DRSGYADEVEELFAQLAAKDVVVRGTYDLSGLRADADVMIWWHSENSDDLQEAYNRFRR
                     TRLGRALEPVWSNMALHRPAEFNKSHIPAFLADEHARDYVSVYPFVRSYDWYLLPDEDR
                     RRMLADHGKMARGFPDVRANTVASFSLGDYEWILAFEADELHRIVDLMRHLRGSEARRH
                     VREEIPFYTGRRKPVAELVAGLA"
     gene            complement(1233..2942)
                     /locus_tag="HRD51_RS30770"
                     /old_locus_tag="HRD51_30390"
     CDS             complement(1233..2942)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (91-511). E-value:
                     5.8e-18. Score: 57.3. Matches aSDomain:
                     nrpspksdomains_HRD51_RS30770_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018536946.1"
                     /locus_tag="HRD51_RS30770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HRD51_30390"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_235094665.1"
                     /sec_met_domain="PF00561 (E-value: 6.1e-21, bitscore: 74.1,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRALALYGALGSLVLSGLASAPAGGAARDPAAPPAPVHPLTFGRC
                     AGVEHLPASVECGTLTVPLDYARPHGKQIKLTVSRTRATSSAERQGALVFNPGGPGASS
                     MQFPLYGALPTWRRTARAYDFVGYAPRGVGRSAPLSCQDPKDFTKAPTDSPRYPSESFK
                     QKKVAEAKAYAHGCAQRAGADLPFYNSINNARDLDMLRAALGEKKLTFMGASYGTYFGA
                     VYATLFPGHVRRMVFDSVVNPDPRQIWYQNNLDQSVAFERRWGDWLRWVAKHDSTYHLG
                     SDKQAVQHSYDTVIQRLRRAPVNGKIGPGQLQAAYLKTGYNDAFWPMRAEALSKYLRGD
                     AQLLIAQALPSGDGQGEENANAVYTAVECNDSAWPRNWHVWDRDNTALARTAPFETWDN
                     AWMNLPCAFWPERRQSAADGVEEAIRTTTRRAVKGASAGDALAHASQQVADTDLDDALY
                     GRGRPLDIRTEPGALPPVLLLAAERDAATPYPGALELQRRLPGSSLVTERDAGTHGIAY
                     GDNLCVNGYVETYLLRGKVPGHRSYCGPRAEPVPGQPLQRARPTLVQQVKGR"
     aSDomain        complement(1410..2669)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_HRD51_RS30770_Abhydrolase_1.1"
                     /evalue="5.80E-18"
                     /label="HRD51_RS30770_Abhydrolase_1.1"
                     /locus_tag="HRD51_RS30770"
                     /protein_end="511"
                     /protein_start="91"
                     /score="57.3"
                     /tool="antismash"
                     /translation="ALVFNPGGPGASSMQFPLYGALPTWRRTARAYDFVGYAPRGVGRS
                     APLSCQDPKDFTKAPTDSPRYPSESFKQKKVAEAKAYAHGCAQRAGADLPFYNSINNAR
                     DLDMLRAALGEKKLTFMGASYGTYFGAVYATLFPGHVRRMVFDSVVNPDPRQIWYQNNL
                     DQSVAFERRWGDWLRWVAKHDSTYHLGSDKQAVQHSYDTVIQRLRRAPVNGKIGPGQLQ
                     AAYLKTGYNDAFWPMRAEALSKYLRGDAQLLIAQALPSGDGQGEENANAVYTAVECNDS
                     AWPRNWHVWDRDNTALARTAPFETWDNAWMNLPCAFWPERRQSAADGVEEAIRTTTRRA
                     VKGASAGDALAHASQQVADTDLDDALYGRGRPLDIRTEPGALPPVLLLAAERDAATPYP
                     GALELQRRLPGSSLVTERDAG"
     gene            complement(3079..3867)
                     /locus_tag="HRD51_RS30775"
                     /old_locus_tag="HRD51_30395"
     CDS             complement(3079..3867)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018536945.1"
                     /locus_tag="HRD51_RS30775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HRD51_30395"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_235094666.1"
                     /transl_table=11
                     /translation="MWLLFLLLAWVAAVVSCVRVCRTAVVTGQAQHRAQDTPPDPAPDA
                     DGAHAGLTLYETAYLSGGPHRLADVVLVLMAQQRRLHLAHTGWATVVDPVGQDPVERAA
                     LTAIGPEGQCRIPAVRTALSGTDAVRGLADRLVDAGLAAPAAIRTAVRQVRGAALVVLL
                     AAGATYWTAPSGEPDRLVLAWFALPLILALGTWAIARTEVRPSTGWATEAGAARLRQEH
                     PGVRDRRPAPEPAGLTGLALHGPAVLVDPGLRAALHSSGA"
     misc_feature    3910..3924
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 16.28"
                     /tool="antismash"
     gene            complement(4005..4991)
                     /locus_tag="HRD51_RS30780"
                     /old_locus_tag="HRD51_30400"
     CDS             complement(4005..4991)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR04222.1"
                     /locus_tag="HRD51_RS30780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HRD51_30400"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_235094667.1"
                     /transl_table=11
                     /translation="MSFTQGVYLGLGLSSLALLVGALVVRLRTPAVGRVAGPTRDVREL
                     AFLAGGPGRVADTALWVMHEDGRLAIGGPGVVTVRRAAAHDPVETAVLDAVAATPGGAL
                     AAVRGRVMRSAAVQEVGDRLAARGLLRPPRAGRGWRLLAGLQVKASVVLFAVALVLSFL
                     AVRDGGGPWAPPVAVRVVPAVAVGLLVGLPCRRLFTRRITRAGRSDLAAARPRGARPDD
                     LRSLEAAWQVAGLAVALGGTAVLADELLRDHFVQAQGAVAASTPSGGSSAGSGGCGGDA
                     GSAWCGSSDGGGDSGCGASSCGGGSSCGGSSGCGGSGCGGGGCGGGD"
     gene            complement(5001..6297)
                     /locus_tag="HRD51_RS30785"
                     /old_locus_tag="HRD51_30405"
                     /pseudo=""
     CDS             complement(5001..6297)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018564414.1"
                     /locus_tag="HRD51_RS30785"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="HRD51_30405"
                     /product="DUF692 domain-containing protein"
                     /pseudo=""
                     /sec_met_domain="DUF692 (E-value: 4.6e-95, bitscore: 316.8,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRRLGTGIGWRPEIAAEIAALPGVDWVEVVAENVCPGHLPDALRE
                     LRDRGTTVIPHGVSLGLGGADRPDAGRLAALAERATALEAPLVTEHIAFVRAGGPLIAS
                     PVLEAGHLLPMPRTRDALDVLCENVRIAQDALPVPLALENIAALFAWPDEELTEGQFLS
                     ELVERTGVGLLIDVANLHTNHVNRGEDPTAALDELPVEALAYVHVAGGVERDGVWHDSH
                     AHPVTRPVLDVLAELCARTDPPGVLLERDDDFPEGGELAGELDAIRAVVEEARRVRAA"
     gene            complement(6454..6675)
                     /locus_tag="HRD51_RS30790"
     CDS             complement(6454..6675)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="HRD51_RS30790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_235094668.1"
                     /transl_table=11
                     /translation="MLTSLIGFTVVAVLLTVSPGPDFAVVLRTALGSGRRAALCRALGQ
                     RPRVRRLDQATGGVLVALGAAVGWEVAR"
     gene            complement(6781..>7098)
                     /locus_tag="HRD51_RS30795"
                     /old_locus_tag="HRD51_30415"
                     /pseudo=""
     CDS             complement(6781..>7098)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006350473.1"
                     /locus_tag="HRD51_RS30795"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="HRD51_30415"
                     /product="TetR-like C-terminal domain-containing protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="SPAPGYHTPEDITGIADRITAVLLDACAALAADEPTTPFGARLEA
                     HREWAHDHPAPPAALHKALAFWSRLHGVLSLALAGQFNGMGLDPALFFAAELDGLLAAG
                     A"
     gene            <7088..7297
                     /locus_tag="HRD51_RS30800"
                     /old_locus_tag="HRD51_30420"
                     /pseudo=""
     CDS             <7088..7297
                     /NRPS_PKS="Domain: Polyketide_cyc2 (6-61). E-value:
                     9.6e-08. Score: 24.5. Matches aSDomain:
                     nrpspksdomains_HRD51_RS30800_Polyketide_cyc2.1"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008741218.1"
                     /locus_tag="HRD51_RS30800"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="HRD51_30420"
                     /product="polyketide cyclase /reductase"
                     /pseudo=""
                     /transl_table=11
                     /translation="GAGLRIDGVHIWNFTEVEGGVRVSTEETRTGAQVDANVPAATAIL
                     RQDLEAWLRELKATAEATTLVQRH"
     aSDomain        7109..7273
                     /aSDomain="Polyketide_cyc"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_HRD51_RS30800_Polyketide_cyc2.1"
                     /domain_subtypes="Polyketide_cyc2"
                     /evalue="9.60E-08"
                     /label="HRD51_RS30800_Polyketide_cyc2.1"
                     /locus_tag="HRD51_RS30800"
                     /protein_end="61"
                     /protein_start="6"
                     /score="24.5"
                     /tool="antismash"
                     /translation="DGVHIWNFTEVEGGVRVSTEETRTGAQVDANVPAATAILRQDLEA
                     WLRELKATAE"
     misc_feature    complement(7112..7127)
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 15.87"
                     /tool="antismash"
     gene            complement(7532..8242)
                     /locus_tag="HRD51_RS30805"
                     /old_locus_tag="HRD51_30425"
     CDS             complement(7532..8242)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014049034.1"
                     /locus_tag="HRD51_RS30805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HRD51_30425"
                     /product="peptidyl-tRNA hydrolase"
                     /protein_id="WP_235096140.1"
                     /transl_table=11
                     /translation="MDDQRAQAPTEPAPEYVLPLVVRVERAAPPGRTDALETAARAVLV
                     LLSDERATDAGEWARAVHDWQDSRIRKVVRRARGAEWRRAGALPGITVTGREAEVRVFP
                     PVPLDGWPKDLARLQVSGTELDEPQAPAEPVDGAPVLWLNPGLKMSAGKAMAQAGHGAQ
                     LAWWELSTAEREAWRAAGFPLAVRTATPAAWPALTTSGLPVVRDAGFTEIAPGKTVVTE
                     GGSRFCPLPRTPRP"
     gene            8468..9376
                     /locus_tag="HRD51_RS30810"
                     /old_locus_tag="HRD51_30430"
     CDS             8468..9376
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018536943.1"
                     /locus_tag="HRD51_RS30810"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HRD51_30430"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_235096141.1"
                     /transl_table=11
                     /translation="MRLDGVGRRYGLRGPWVLREVRLDVPAGALLRIEGTNGSGKSTLL
                     RLLAGIDRPTTGRITGRPRTAYVPERFPAALPFTAAGYLAHLGRVHGLRTPEAADRAAE
                     WLARFGAAGHARTPLAELSKGTSQKVAVAQALLAEPELLVLDEAWTGLDPAARDTLDRA
                     VAERVAAGGTVVFVDHDPRRLAGAASARYRVADGRLLPRERGTAAEPADGVDAGAGPRT
                     VVEAEGPPGSPPPSGLPGAPDLAPGPGNSLRLTVATVHSDALLRALLTARPPWHIRAVA
                     ADEPPGPGTPGTPAPGEAPRP"
     gene            9373..10053
                     /locus_tag="HRD51_RS30815"
                     /old_locus_tag="HRD51_30435"
     CDS             9373..10053
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016576543.1"
                     /locus_tag="HRD51_RS30815"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HRD51_30435"
                     /product="ABC transporter"
                     /protein_id="WP_235094669.1"
                     /transl_table=11
                     /translation="MTASLPALVRYQAALLVGSYRWLPPLLAYAAFLAIGVQTGGPILD
                     AYGYAAGGLLPVSVWLARICATNEPPAARSCVAAAAGPGRAHLAALLAAVGAALVLALA
                     GAAVVALLSDPHSTGHRVAVPVLPATGAGLLTALVCLLLGIGIGALCSPPVLRRPGWGV
                     PVGLIAALLVLVLGASPANAALRGMITGSERGTVTVPWLSLAVAVPLASAATALACALG
                     GRRG"
     gene            10085..10282
                     /locus_tag="HRD51_RS30820"
                     /old_locus_tag="HRD51_30440"
     CDS             10085..10282
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005481904.1"
                     /locus_tag="HRD51_RS30820"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HRD51_30440"
                     /product="hypothetical protein"
                     /protein_id="WP_205367276.1"
                     /transl_table=11
                     /translation="MPRTDPHPTPDPDGPGPEAPGADAGEPRADCVECGRPTAYPASRP
                     GIVRCPVCEWQEAQRSACSG"
ORIGIN
        1 ggatcaaggc gtcgaccttc gccagccaca agtggaactg gatcgccgag gccgacccgg
       61 acctcttcgt gctgcgcacc tccctcggcc ggtacggcga cgaggccgat ctggagcgcg
      121 acgacgccga actggtcgcc ctctccctcg ccgacctcgg cgaggcggtc ggcctgaccg
      181 cccggccggt ggccaccacc gtcacccgct ggggcggcgg gctgccgcag tacgccgtcg
      241 gccatgtgga ccgggtggcg cgcatccggg ccgcgatcgc cggactgccg ggcccgctgc
      301 gggtctgcgg cgcgctctac gacggcgtcg gcatcccggc gtgcatcgcc tccgcgcacc
      361 gggccgccga cgaggtcctc gggaccctga cgccgggcgt cgccggcggc gagtgagaat
      421 ggacgacatg acagacgctt ccacccccgc ctccgacaag atccccaacg ccggcaagaa
      481 ggccaaggac ctcaacgagg tcatccgcta caccctctgg tcggtcttca agctgcgcga
      541 cgtccttccc gaggaccggt ccggctacgc cgacgaggtc gaggagctgt tcgcccagct
      601 cgccgccaag gacgtggtgg tccgcggcac ctacgacctc tccgggctgc gcgccgacgc
      661 cgacgtgatg atctggtggc actcggagaa ctccgacgac ctccaggagg cgtacaaccg
      721 cttccgccgc acccgtctgg gccgcgcgct ggagccggtc tggtcgaaca tggcgctgca
      781 ccgtcccgcc gagttcaaca agtcgcacat cccggccttc ctggccgacg agcacgcccg
      841 cgactacgtg agcgtctacc cgttcgtgcg ctcctacgac tggtacctcc tccccgacga
      901 ggaccgccgc cggatgctcg ccgaccacgg caagatggcc cgcggcttcc ccgacgtccg
      961 cgccaacacc gtcgcctcct tctcgctcgg cgactacgag tggatcctcg ccttcgaggc
     1021 cgacgagctc caccgcatcg tcgacctgat gcgtcacctg cgcggctccg aggcgcgccg
     1081 gcacgtccgc gaggagatcc cgttctacac cggccgccgc aagccggtcg ccgagctggt
     1141 cgccggcctg gcctgaccgg tcggacaccc cggaacacgg aggtcggggc cgcccccgaa
     1201 gggacggccc cgacctccac gctccggggc ggctaccgcc ccttcacctg ctgcaccagc
     1261 gtcggccggg cccgctggag cggctgcccc ggcaccggct cggcgcgcgg tccgcagtac
     1321 gaccggtgcc cggggacctt cccgcgcagc aggtacgtct cgacgtagcc gttcacacag
     1381 aggttgtcgc cgtacgcgat gccgtgggtg cccgcgtccc gctcggtcac cagggacgag
     1441 ccgggcagcc gccgctgcag ctccagcgca cccgggtagg gggtggccgc gtcccgttcg
     1501 gcggccagca gcagcaccgg cggcagcgcg ccgggctcgg tgcggatgtc cagcgggcgg
     1561 ccccgcccgt acagcgcgtc gtccaggtcg gtgtcggcga cctgctggga ggcgtgggcc
     1621 agggcgtccc cggccgacgc gcccttgacg gcccgccggg tggtggtgcg gatcgcctcc
     1681 tcgacgccgt ccgccgccga ctgccggcgc tccggccaga aggcgcacgg caggttcatc
     1741 caggcgttgt cccaggtctc gaacggcgcg gtgcgggcca gggcggtgtt gtcccggtcc
     1801 cagacgtgcc agttgcgcgg ccaggccgag tcgttgcact cgacggcggt gtagacggcg
     1861 ttggcgttct cctcgccctg gccgtcgccg gagggcagtg cctgggcgat caacagctgg
     1921 gcgtcgccgc gcaggtactt cgacagcgcc tcggcgcgca tcggccagaa cgcgtcgttg
     1981 tagccggtct tgaggtacgc cgcctggagc tgcccggggc cgatcttccc gttgaccggc
     2041 gcgcgccgca gccgctggat gacggtgtcg taggagtgct gcaccgcctg cttgtcggat
     2101 cccaggtggt aggtggagtc gtgcttggcg acccagcgca gccagtcgcc ccagcggcgc
     2161 tcgaaggcga cgctctggtc gaggttgttc tggtaccaga tctgccgggg atcggggttg
     2221 accacgctgt cgaagaccat ccggcggaca tggccgggga acagcgtcgc gtagaccgcc
     2281 ccgaagtacg tcccgtagga cgcgcccatg aacgtcagct tcttctcgcc gagcgccgcc
     2341 cgcagcatgt ccaggtcccg ggcgttgttg atcgagttgt agaacggcag gtcggcgccg
     2401 gcccgttggg cgcagccgtg ggcgtacgcc ttcgcctccg ccaccttctt ctgcttgaag
     2461 gactcgctgg ggtaccgcgg gctgtccgtg ggcgccttgg tgaagtcctt ggggtcctgg
     2521 caggacagcg gggcgctgcg ccccaccccg cgcggcgcat agccgacgaa gtcgtaggcg
     2581 cgggcggtcc ggcgccaggt cggcagcgct ccgtagaggg ggaactgcat gctggacgcg
     2641 ccggggccgc cggggttgaa gaccagggcg ccctgccgtt cggcggacga ggtggcccgg
     2701 gtccggctga ccgtcagctt gatctgcttg ccgtgcggcc gggcgtagtc gagcgggacg
     2761 gtgagcgtgc cgcactcgac ggaggccggg aggtgctcca cgcccgcgca ccggccgaag
     2821 gtgagggggt gaacgggggc gggcggcgcc gccgggtccc gggcggcgcc gccggccggt
     2881 gccgaggcga ggccgctcag gaccagcgac ccgagggcgc cgtacaacgc aagtgctctc
     2941 acgagccgtc ccttcgtatg gtgcgcggtg cggggctccg gggaggggct cgctgcacgg
     3001 ggtcgcgcgg tgggcataca aaagggatag ttgggccaac cgagggcgaa gtaaagcacc
     3061 gtacggcggt gtcggacgct atgccccgga agagtggagc gccgcgcgca aaccgggatc
     3121 gacgagcacg gcgggtccgt gcagggcgag tcccgtcagg ccggccggtt ccggcgccgg
     3181 acgtctgtcc cgcacgccgg ggtgctcctg ccgcagccgt gccgcgccgg cctcggtggc
     3241 ccagccggtg gacggacgga cctccgtgcg ggcgatggcc caggtgccga gggcgaggat
     3301 cagcgggagc gcgaaccacg ccagcaccag ccggtcgggc tcgccggacg gggccgtcca
     3361 gtaggtcgcg ccggcggcca acagcaccac gagggcggcg ccgcggacct ggcggaccgc
     3421 ggtgcggatc gcggcgggcg ccgcgagacc ggcgtcgacc agccggtcgg ccagcccccg
     3481 gaccgcatcg gtgccggaca gggcggtccg gacggcgggt atgcggcact gcccctcggg
     3541 gccgatggcg gtgagcgcgg cccgctcgac ggggtcctgg ccgaccgggt cgacgaccgt
     3601 ggcccagccg gtgtgggcga ggtgcagccg ccgctgttgg gccatcagga cgagcaccac
     3661 atcggcgagt cggtgcgggc cgccggagag ataggccgtc tcgtacaggg tcagcccggc
     3721 gtgggcgccg tcggcgtccg gtgcggggtc cggcggggtg tcctgggcgc ggtgctgggc
     3781 ctggccggtg acgaccgccg tacggcagac ccggacacag gagacgaccg ccgccaccca
     3841 ggcgagcagg aggaagagga gccacatagt ggacgttcta cgggacggcg ggcgtggccg
     3901 ccatgacgtt tctcaccttg tggagagtgc gccccggccg gtcggggcgc cgaactcgcc
     3961 gccccgaccg gccccttcgt gcggacaccc gatctgacgg ttcgtcagtc accgccgccg
     4021 cagcccccgc cgccgcaccc ggagccgcca cagcccgaac tcccgccgca cgacgacccg
     4081 ccgccgcacg aggacgcccc gcatccggag tccccaccgc cgtccgagga gccgcaccac
     4141 gccgagcccg cgtccccgcc gcacccgccg gagcccgccg acgacccgcc ggacggcgtc
     4201 gaggccgcca ccgccccctg ggcctgcacg aagtggtccc gcagcaactc gtccgcgagc
     4261 accgcggtac cgcccagcgc caccgccagc ccggcgacct gccaggccgc ctccaggctc
     4321 cggaggtcgt cgggccgggc gccccggggc cgggccgcgg ccaggtcgga ccggcccgcc
     4381 cgggtgatcc ggcgggtgaa caaccgccgg cacggcagcc cgaccagcag acccacggcc
     4441 accgccggga cgacccggac ggccaccggc ggtgcccacg gaccaccgcc gtcgcggacg
     4501 gcgaggaagg acagcaccag cgcgacggcg aagagcacga cgctcgcctt cacctggagc
     4561 ccggccagca gccgccagcc ccggccggcg cgcggcgggc gcagcagccc tcgggccgcc
     4621 agtcggtcgc cgacctcctg caccgccgca ctgcgcatca cccgcccgcg gacggcggcc
     4681 agcgccccgc ccggggtggc ggcgaccgcg tccagcacgg ccgtctccac cgggtcgtgc
     4741 gcggcggcgc gccgcaccgt caccacgccc gggccgccga tcgccagccg cccgtcctcg
     4801 tgcatcaccc agagcgcggt gtccgccacc cggcccggcc cgccggccag gaacgccaac
     4861 tcccggacgt cacgggtcgg tccggccacc cgcccgaccg ccggcgtccg cagccgcacc
     4921 accagggccc cgacgagcag cgccagcgac gacaggccga ggcccagata caccccttgt
     4981 gtgaacgaca cggcggcacc tcacttccgg cgcagcgcgc gctgcaggat tcctgcccgc
     5041 cgggcggcgg cccgcgcgag ctgcgccgcg cggcgcccgg acggcggttg cggaccggcg
     5101 cggtcgcgcc accagtgcgt cagccgccgc cgtgccaccg ggtccgcggg gcggtcctgc
     5161 gccagcagga acgccgcgaa gtccagggcg tcctggcggt agccgccggc catggggcgg
     5221 cgccgggcgt agccgaggaa ggccgaccgg tagccgtcgc cgaggatctc cgtcagctcc
     5281 ggtgccacct tggcgactac cgaggcccgc ttggcggcca gggcccggct ctgcacccgc
     5341 agccgggccg ggtcgaagcc ctccggggcg ggcgtcccgg ccaccagtgc ggacagcagc
     5401 gcggcctgcg ccagcgccaa ccgctcacgg gccggaccgg tgtccgccac cgcctcaagc
     5461 cgcccggaca cggcgcgcct cctccaccac ggcgcggatc gcgtcgagtt caccggccag
     5521 ctcgccgccc tcggggaagt cgtcgtcgcg ctccagcagc acgcccggcg gatcggtccg
     5581 ggcgcacagc tccgcgagga cgtccaggac cggccgggtc accgggtggg cgtggctgtc
     5641 gtgccacacc ccgtcccgct cgacgccgcc cgcgacatgg acgtaggcga gcgcctcgac
     5701 cggcagctcg tcgagggccg cggtcgggtc ctcgccacgg ttgacgtggt tggtgtggag
     5761 gttggcgacg tcgatcagca gcccgacccc ggtgcgctcg accagctcgg acaggaactg
     5821 cccctcggtc agctcctcgt cgggccaggc gaacagcgcg gcgatgttct ccagggccag
     5881 cggcaccggc agcgcgtcct gggcgatccg gacgttctcg cacagcacgt ccagcgcgtc
     5941 ccgggtgcgc ggcatcggca gcaggtgccc cgcctccagc accggcgacg cgatcagcgg
     6001 gccgccggcg cggacgaacg cgatgtgctc ggtgaccagc ggggcctcca gtgcggtggc
     6061 ccgctcggcg agggccgcca gccgcccggc gtccggccgg tccgcgccgc cgagcccgag
     6121 cgagaccccg tgcgggatca ccgtcgtgcc gcgatcccgc aactcccgca gggcgtccgg
     6181 gagatggccg gggcagacgt tctcggccac cacctccacc cagtccaccc cgggcagcgc
     6241 ggcgatctcc gcggcgatct ccggccgcca gccgatgccc gtccccaaac gacgcatggt
     6301 tccccctttg tgctccccgc ggtcggggta gtcctgaccc ggcgcgggcg gggcgaatcc
     6361 cgcacccgcg cggttcagag gttgatttga gcttctctca ccagaggccg gcccggcccg
     6421 tgccgtgcgc cggccgttgc gtcccgccgc ggctcagcgg gcgacctccc acccgaccgc
     6481 cgcgcccagc gccaccagca ccccgccggt ggcctggtcc agccgccgca cccgcgggcg
     6541 ctgccccagc gcacggcaca gcgcggcgcg ccgccccgag ccgagcgcgg tccgcagaac
     6601 caccgcgaag tccgggccgg gggagacggt gagcagtacg gccaccacgg tgaagccgat
     6661 gagcgaggtc agcatgggtg acacggtatg tcccgccgtg ggccgggccg ccggcgggga
     6721 cgggggagcg gcccaccggg gcccgccgcc ggcgacccgg ccgtcgcccc gcgcacgacg
     6781 tcacgctccg gcggccaaca ggccgtccag ttcggccgcg aagaacaggg cggggtccag
     6841 gcccatgccg ttgaactggc cggcgagcgc cagggacagg acaccgtgca accgggacca
     6901 gaaggccaga gctttgtgga gagccgccgg tggggcgggg tggtcgtggg cccattcccg
     6961 gtgcgcttcc aggcgggcgc cgaacggtgt cgtcggttcg tccgcggcga gggctgcgca
     7021 ggcgtcgagc agaacggccg tgatccggtc cgcgatgccg gtgatgtcct cgggggtgtg
     7081 gtagccgggc gcggggctgc gcatcgacgg cgtccacata tggaacttca ccgaggtcga
     7141 gggcggggtc cgggtgagca ccgaggagac ccgcaccggc gcccaggtcg acgcgaacgt
     7201 tcccgccgcg accgcgatcc tgcgtcagga cctcgaagca tggttgcgcg agctgaaggc
     7261 caccgccgaa gcaaccaccc tcgttcagcg gcactgaacc ccgacgcccc gattgtgcga
     7321 cgagcgcgga actcaccgtc gaaatcggcc agttcgcgac ggatggcgcg gctgacgcag
     7381 atgacgcgga tggcgcgggt gcgcgggatg gtggagaggg gcatccgaca ggggcggcgg
     7441 accttcgatc gggtccccgg cgtcccgagg gccgacttcg gtcgggcgcc gtccggaacg
     7501 cgggcagccc gtggtgcgca atccgcacga cctacggccg tggtgtgcgg gggagggggc
     7561 agaatcggct gcccccttcg gtcaccacgg ttttcccggg cgcgatctcg gtgaacccgg
     7621 cgtcccgcac cacgggcagt ccgctggtcg tcagtgccgg ccaggccgcg ggcgtcgcgg
     7681 tgcgcacggc cagcgggaac cctgccgcgc gccaggcttc ccgctccgcc gtggacagct
     7741 cccaccaggc cagctgtgcg ccgtgcccgg cctgtgccat cgccttgccc gccgacatct
     7801 tcagcccggg attgagccac agcaccggcg cgccgtccac gggctccgcg ggggcctgcg
     7861 gttcgtccag ctcggtgccg gagacctgga gccgggcgag gtccttcggc cagccgtcca
     7921 gcggcaccgg cgggaagacc cgcacctcgg cctcgcggcc ggtgaccgtg atgccgggca
     7981 gggccccggc ccgccgccac tccgctccgc gcgcccgccg gaccaccttc cgtatccggg
     8041 agtcctgcca gtcgtgcacc gcccgggccc actcgcccgc gtcggtggcc cgctcgtcgg
     8101 agagcagcac cagcaccgcc cgcgccgcgg tctccagcgc gtccgtgcgc ccggggggtg
     8161 cggcccgctc cacgcgcacc accagcggca ggacgtactc cggcgccggc tccgtggggg
     8221 cctgcgcccg ctggtcgtcg atccgggtgg gcgcgctgtg ctcgccggac gggccggtgg
     8281 tctcggtact ggtcacccga tcagtctgcc agcagccacc gacagtcccc ccccctcgcg
     8341 cccgccgacg gtcgccgaac ccgcgcccgc cgacgcggtg ctgcccgcgc ccggctaccg
     8401 tcggcggcta caggggaaca ggggagcggg ggaggtgcgg ccgatgccgc ggcaggaagc
     8461 gcagcggatg cggctggacg gggtcgggcg gcgctacggc ctgcgcgggc cctgggtgct
     8521 gcgcgaggtg cggctcgatg tgccggcggg cgcgctgttg cgcatcgagg ggaccaacgg
     8581 cagcggcaag tccacgctgc tgcggctgct cgccggcatc gatcggccca ccacgggtcg
     8641 gatcaccggg cgtccgcgca ccgcctacgt gcccgagcgg ttcccggccg ccctgccgtt
     8701 caccgccgcc ggttacctcg cccacctcgg ccgcgtccac ggcctgcgga caccggaggc
     8761 ggcggaccgt gccgccgagt ggctggcgcg cttcggcgcc gccggtcacg cccggacccc
     8821 gctggcggag ctgtccaagg ggacgagcca gaaggtcgcg gtcgcccagg cactgctcgc
     8881 cgagcccgaa ctcctcgtcc tggacgaggc gtggaccggg ctggacccgg ccgcccgcga
     8941 caccctcgac cgcgcggtcg ccgaacgggt cgccgccggc ggcaccgtgg tcttcgtcga
     9001 ccacgacccg cgccgactgg ccggggccgc gagcgcccgc taccgcgtcg ccgacggccg
     9061 gctgctgccc cgggagcgcg gcaccgccgc cgagccggcc gacggggtcg acgccggtgc
     9121 cggcccgcgg accgtcgtcg aggcggaggg tccgccgggc agcccgccgc cgtccggtct
     9181 gccgggcgcg ccggacctcg cccccggccc cggcaacagc ctgcggctga ccgtcgccac
     9241 cgtccactcc gacgccctgc tgcgcgccct gctcaccgcg cgcccgccgt ggcacatccg
     9301 ggcggtcgcc gccgacgagc cgcccggccc cggaaccccc ggaacccctg ctcccggaga
     9361 ggctccccgt ccatgaccgc ctccctgccg gcgctggtcc gctatcaggc ggcgctgctc
     9421 gtcggctcct accgttggct gccgccgctc ctcgcctacg cggcgttcct cgcgatcggc
     9481 gtccagaccg gcggcccgat cctggacgcg tacggctacg ccgccggcgg gctgctgccg
     9541 gtctccgtct ggctggcccg gatctgcgcc accaacgagc cgccggccgc ccgcagttgc
     9601 gtggcggccg ccgccggacc cggccgggcg cacctcgccg cgctgctggc cgcggtcggc
     9661 gcggcgctgg tcctcgcact cgccggggct gccgtcgtcg cgctgctctc cgacccgcac
     9721 tcgaccggtc accgggtcgc ggtcccggtg ctgcccgcga ccggcgccgg gctgctcacc
     9781 gcgttggtct gcctcttgct gggcatcggg atcggcgcgc tgtgctcccc gccggtgctg
     9841 cgccgccccg gctggggcgt cccggtcgga ctgatcgccg cgctgctggt cctcgtgctg
     9901 ggtgcgtcgc ccgccaacgc cgcgctgcgc ggcatgatca ccggctccga gcggggcacc
     9961 gtcaccgtgc cctggctgtc gctcgccgtc gcggtgccgc tggcgtccgc agccacggcc
    10021 ctggcctgcg ccctcggcgg gcgccgcggc tgagggctcc gggtccgcgg cgtaggctcg
    10081 ccccatgccc cgcaccgatc cgcaccccac gcccgacccg gacggcccgg gcccggaggc
    10141 ccccggcgcc gacgcgggcg agccccgcgc cgactgcgtc gagtgcggcc gtccgaccgc
    10201 atacccggcg tcccggccgg gcatcgtccg ctgcccggtc tgcgagtggc aggaggccca
    10261 gcgctcggcc tgctcgggct gacccgcccg gcgcccgccg ggcgaccggc cgccggcgcc
    10321 ggctacgcca ccagcgtctt ggcgtcccgg gccagcgcgg tcagccggga gatggcgcgg
    10381 aagtacttct tccggtagcc gccgcgcagc atctcgtcgc tgaacagctc gtcgaagggc
    10441 ttgccggagg ccagcaccgg cacctcccgg tcgtagagcc ggtcggcgag caccaccaga
    10501 cgcagcgcgg tggactggtc cggaaccgcc tgcacgtcgg tgaggcagac cgccgctatg
    10561 ccgtcgcaca tcgcgccgta ccggctgggg tgcaccgtcg agaggtggtg caggagggcc
    10621 gggaagtcgt cgagcgaggc gccgggggtg tggtgcgccg cgcgggccac cgtctcgtcg
    10681 tcgtacgggg cgggcgcctc gggcaggccg cggtgccggt agtcctcgcc gtcgatgcgc
    10741 agggcgcgga agtgggcgct caggccctgg atctcgcgca ggaagtcggc ggcggcgaac
    10801 cggccctcgc cgagcttgcc gggcagcgtg ttggaggtgg cggccagcgc cacaccggac
    10861 tccaccagct tgcccagcag ggtggagacc agcaccgtgt cgccggggtc gtccagctcg
    10921 aactcgtcga tgcacagcag ccggtgggag ccgagcgtgc gcacggtctg ctggaagccg
    10981 agcgcgccga ccaggttggt cagctcgacg aaggtcccga acgccttgag ctcgggggcg
    11041 gcgggggtcg cgtgccagag ggaggcgagc aggtgggtct tgccgacgcc gtagccgccg
    11101 tccagataga ccccgcgcgg gctcttgggt gcggcggtct tcttcggtcc ccgggagaac
    11161 cagcgccgct tcccgccggt gtccgtgtcc gcgccgtcga tgcccgcggc gaacgtccgc
    11221 aggacctgca cggcctcggc ctggctgggc tgccccgggt ccggcaggta ggtgtcgaag
    11281 cgggcgccct ggaagcg
//
