LOCUS       NZ_CP054019            10405 bp    DNA     linear   CON 28-JAN-2024
DEFINITION  Streptomyces sp. A1-5 chromosome.
ACCESSION   NZ_CP054019
VERSION     NZ_CP054019.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. A1-5
  ORGANISM  Streptomyces sp. A1-5
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 9459690)
  AUTHORS   Dashti,Y., Sumang,F.A.M., de los Santos,E.L.C., Alcantara,E.P. and
            Challis,G.L.
  TITLE     Structural revision and biosynthesis of polyketides FXJ15321 and
            FXJ15322 from Streptomyces sp. A1-5
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9459690)
  AUTHORS   de los Santos,E.L.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2020) Warwick Integrative Synthetic Biology
            Centre, University of Warwick, Gibbet Hill Road, Coventry CV4 7AL,
            United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: JUN-2019
            Assembly Method                   :: Flye v. 2.4.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Oxford Nanopore MiniION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/28/2024 04:28:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,328
            CDSs (total)                      :: 8,230
            Genes (coding)                    :: 7,847
            CDSs (with protein)               :: 7,847
            Genes (RNA)                       :: 98
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 156 of 383
            Pseudo Genes (incomplete)         :: 280 of 383
            Pseudo Genes (internal stop)      :: 26 of 383
            Pseudo Genes (multiple problems)  :: 73 of 383
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 22:08:01
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2621043
            Orig. end                         :: 2631448
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP054019.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Philippine National
            Collection of Microorganisms.
FEATURES             Location/Qualifiers
     region          1..10405
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="14"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10405
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10405
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5405](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5405
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            complement(197..1177)
                     /locus_tag="HRD51_RS11810"
                     /old_locus_tag="HRD51_11595"
     CDS             complement(197..1177)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018536882.1"
                     /locus_tag="HRD51_RS11810"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HRD51_11595"
                     /product="SCO1860 family LAETG-anchored protein"
                     /protein_id="WP_235093020.1"
                     /transl_table=11
                     /translation="MSTRSTAGSAGTTGRIGAALAAAVLTAGPAVALAAAPAAYATGDD
                     ARGHGTSGAVVLRTALDVNLLDKSLNVPVRAVLNEVHAPASADKTALTVRLDGIDGGRP
                     VSLLRADVATARATADGRTAEGHAHLVRAKVQLPGLPALPLAEVQQVTARAVCAAGAHP
                     RAESQVLGDVVVLGKRVALRAGGTTEVDVPGVGDVRLDLSQKSTTSASAAATALQLRVS
                     VNPLKLNVAEVHGQVTLAQATCTTPHGTPDKPGTPAHPGQPHEPGTGTQAHTGGGARPA
                     TENLAETGGSAATPYLVGGAVLLVAGGAGAMVYTRRRSAAPDGRG"
     gene            complement(1441..2532)
                     /locus_tag="HRD51_RS11815"
                     /old_locus_tag="HRD51_11600"
     CDS             complement(1441..2532)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016576771.1"
                     /locus_tag="HRD51_RS11815"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HRD51_11600"
                     /product="amidohydrolase family protein"
                     /protein_id="WP_205369744.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 7.7e-17, bitscore:
                     60.4, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSEGAVLHLKGRVLVGPDDVRDELWVIDGRVTFERPALARDARTV
                     TGWVLPGLVDAHCHVGLDAHGPVDAATSEKQALTDRDAGTLLIRDAGSPSDTRWIDDRP
                     DLPRIIRAGRHIARTRRYIRNYAHEIEPADLVAYVAQEARRGDGWVKLVGDWIDRSTGD
                     LAACWPPGEVAAAIAEAHRLGARVTAHCFAEESLAPLVEAGIDCIEHATGLTEDTIPLF
                     AERRVAIVPTLVNIATFPQLAAGGETKFPRWADHMRRLHARRYDTVRAAYDAGVPIFAG
                     TDAGGSLPHGLVADEVAELMKAGLPVEAALSATTWGAREWLGRPGLTEGASADLVVYET
                     DPRADVRVLAAPRLVVLRGRVVG"
     misc_feature    2615..2628
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 15.25"
                     /tool="antismash"
     misc_feature    complement(2617..2630)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 14.37"
                     /tool="antismash"
     misc_feature    2836..2850
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 14.87"
                     /tool="antismash"
     misc_feature    complement(2860..2875)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.62"
                     /tool="antismash"
     misc_feature    complement(2870..2885)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 16.52"
                     /tool="antismash"
     misc_feature    2923..2935
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 16.37"
                     /tool="antismash"
     misc_feature    3004..3016
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 17.04"
                     /tool="antismash"
     misc_feature    3027..3041
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 18.04"
                     /tool="antismash"
     gene            3041..3607
                     /gene="ectA"
                     /locus_tag="HRD51_RS11820"
                     /old_locus_tag="HRD51_11605"
     CDS             3041..3607
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018536877.1"
                     /locus_tag="HRD51_RS11820"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HRD51_11605"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_205364121.1"
                     /transl_table=11
                     /translation="MTAAQADHAGARSEIREIPEGFKLDTPRVEDGAAIWRIARDSKTL
                     DLNSSYSYLLWCRDFAATSAVARDATGEPAAFITGYLRPDRPDTLVVWQVAVDAAHRGR
                     GLAAALLDGLTTRAVDELGVRRVETTISPGNTASNRLFASFAERHSVPIEREMLFDAAL
                     FPEQGHEAEVLHLIGPFEAPPGSRR"
     gene            3677..4945
                     /gene="ectB"
                     /locus_tag="HRD51_RS11825"
                     /old_locus_tag="HRD51_11610"
     CDS             3677..4945
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-365). E-value: 4.2e-74.
                     Score: 241.5. Matches aSDomain:
                     nrpspksdomains_HRD51_RS11825_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448159.1"
                     /locus_tag="HRD51_RS11825"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HRD51_11610"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_205364123.1"
                     /sec_met_domain="Aminotran_3 (E-value: 9.3e-76, bitscore:
                     254.0, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTLTQPDLSVFETVESEVRSYCRGWPTVFDRAQGSRLTDEDGHTY
                     LDFFAGAGSLNYGHNNAVLKRALLDYIERDGITHGLDMSTTAKRAFLESFQNIILRPRD
                     LPYKVMFPGPTGANSVEAALKLARKVKGRESIVSFTNAFHGMSLGALAVTGNSMKRAGA
                     GIPLVHGTPMPFDNYLDGQTPDFLWFERLLEDQGSGLNKPAAVIVETVQGEGGINVART
                     EWLRALAALCQRQDMLLIVDDIQMGCGRTGAFFSFEEAGIVPDIVTVSKSISGYGLPMA
                     LTLFKPELDIWEPGEHNGTFRGNNPAFVTAAAALDTYWADGQMEKQTLARGEIVEAHLK
                     AIAEEHPGTFAEYRGRGLVWGLECTDKSLADKIAGRAFELGLLIETSGPQSEVVKLLPA
                     LTTTPEELDEGLRILARAVRDCA"
     aSDomain        3761..4771
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_HRD51_RS11825_Aminotran_3.1"
                     /evalue="4.20E-74"
                     /label="HRD51_RS11825_Aminotran_3.1"
                     /locus_tag="HRD51_RS11825"
                     /protein_end="365"
                     /protein_start="28"
                     /score="241.5"
                     /tool="antismash"
                     /translation="FDRAQGSRLTDEDGHTYLDFFAGAGSLNYGHNNAVLKRALLDYIE
                     RDGITHGLDMSTTAKRAFLESFQNIILRPRDLPYKVMFPGPTGANSVEAALKLARKVKG
                     RESIVSFTNAFHGMSLGALAVTGNSMKRAGAGIPLVHGTPMPFDNYLDGQTPDFLWFER
                     LLEDQGSGLNKPAAVIVETVQGEGGINVARTEWLRALAALCQRQDMLLIVDDIQMGCGR
                     TGAFFSFEEAGIVPDIVTVSKSISGYGLPMALTLFKPELDIWEPGEHNGTFRGNNPAFV
                     TAAAALDTYWADGQMEKQTLARGEIVEAHLKAIAEEHPGTFAEYRGRGLVWGLECT"
     gene            5001..5405
                     /locus_tag="HRD51_RS11830"
                     /old_locus_tag="HRD51_11615"
     CDS             5001..5405
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019358304.1"
                     /locus_tag="HRD51_RS11830"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HRD51_11615"
                     /product="ectoine synthase"
                     /protein_id="WP_039637583.1"
                     /sec_met_domain="ectoine_synt (E-value: 1.5e-51, bitscore:
                     172.4, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFQEIEGTDRHVKAKSGTWESKRIVLAKERVGFSLHETVLY
                     AGTETSMWYANHIEAVVCVEGVAELTNDETGEKHLITPGVMYLLDGHEKHTMRIKEDFR
                     CLCVFNPPVTGREDHDENGVYPLLTEPEAG"
     gene            5476..6378
                     /gene="thpD"
                     /locus_tag="HRD51_RS11835"
                     /old_locus_tag="HRD51_11620"
     CDS             5476..6378
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828636.1"
                     /locus_tag="HRD51_RS11835"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HRD51_11620"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_205364125.1"
                     /sec_met_domain="PhyH (E-value: 1.2e-39, bitscore: 135.6,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTAPERTADLYPTRGTSEVITPRKDPVVWSRPGVEGPFAAAELS
                     DFDRDGFCALPELLTADEVAVYRAELDRLVGDPAMRADPRSIVEPTSQAVRSVFEVHRI
                     SEVFAGLVADPRVVGRARQILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLP
                     HMRTVSVSIALTENYDTNGGLMIMPGSHRYFVGCEGATPKDNYKKSLQMQDAGTPSDEA
                     LTTMADRHGIRLFTGRAGSATWFDCNAMHGSGDNITPYPRSNVFIVFNSVENPAVEPFA
                     APVRRPEYIGARAPFVPVK"
     gene            complement(6520..7593)
                     /locus_tag="HRD51_RS11840"
                     /old_locus_tag="HRD51_11625"
     CDS             complement(6520..7593)
                     /GO_function="GO:0010181 - FMN binding [Evidence IEA]"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1217: NADH:flavin oxidoreductase/NADH oxidase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016574047.1"
                     /locus_tag="HRD51_RS11840"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HRD51_11625"
                     /product="alkene reductase"
                     /protein_id="WP_235093021.1"
                     /transl_table=11
                     /translation="MTTAFDPIELAGIPLANRIVMAPMTRNRATAEGVPTPSMVEYYTQ
                     RASAGLIITEATQTEVVGRGYPLTPGLHTADQVAGWRRVTDSVHAAGGRIFAQLWHGGR
                     IGHPVLLPDGLEPVGPSAVKAAGQGFTQNGPTDFVTPRELTDEQIRATVDGFAAAARNA
                     IEAGFDGVEIHGANGYLIHQFLAPNANRRTDAWGGSDEARARFAIEVVKAVAGAVGAHR
                     TGLRLSPGNPFNDIDEPAPEATYTALIEAVAPLGLAYLHTCDVTDLELLARLRKRFDGP
                     FLLNPATEGRPTGPEELPLVERGEADLLAYGALFIANPDLPRRLAAGGPFNTPDRDGFY
                     GGDDRGYLDYPALDDES"
     gene            complement(7727..8140)
                     /locus_tag="HRD51_RS42110"
     CDS             complement(7727..8140)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="HRD51_RS42110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_328285939.1"
                     /transl_table=11
                     /translation="MPAHAGWVAGEDPGESNYGPVDRPAATARRYDEPHAHYSYVAAEH
                     SLGLLAELGVDTVHAHNVALAQQYRAGLEAAGFTPRAAPGSAIVSTPGLADAEPRLARA
                     GVRVSVRGGLLRAAFHLYNSTEDVSRALSLLVP"
     gene            complement(<8239..8799)
                     /locus_tag="HRD51_RS42115"
                     /pseudo=""
     CDS             complement(<8239..8799)
                     /NRPS_PKS="Domain: Aminotran_5 (16-186). E-value: 2.2e-14.
                     Score: 45.1. Matches aSDomain:
                     nrpspksdomains_HRD51_RS42115_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_1_2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /locus_tag="HRD51_RS42115"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /pseudo=""
                     /sec_met_domain="Aminotran_1_2 (E-value: 9.2e-05, bitscore:
                     20.7, seeds: 48, tool: rule-based-clusters)"
                     /sec_met_domain="Aminotran_5 (E-value: 3e-09, bitscore:
                     35.2, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQRHMALGGDEFAPETVYLNSASCGLLPARSAAALTAAVAESASH
                     GTMGRDYLGTGARVRGAFARLMGVPEERVALGSSVAVQSAFVAGSLPAGSEVLVAEGDF
                     SSLVNPLAARAGCTVRAVPWEALADAVRPGTALVAVSAVHALDGRIADLAAIRDAARAH
                     GALTYVDITQAAGWLPLRLADFDY"
     aSDomain        complement(8242..8751)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_HRD51_RS42115_Aminotran_5.1"
                     /evalue="2.20E-14"
                     /label="HRD51_RS42115_Aminotran_5.1"
                     /locus_tag="HRD51_RS42115"
                     /protein_end="186"
                     /protein_start="16"
                     /score="45.1"
                     /tool="antismash"
                     /translation="VYLNSASCGLLPARSAAALTAAVAESASHGTMGRDYLGTGARVRG
                     AFARLMGVPEERVALGSSVAVQSAFVAGSLPAGSEVLVAEGDFSSLVNPLAARAGCTVR
                     AVPWEALADAVRPGTALVAVSAVHALDGRIADLAAIRDAARAHGALTYVDITQAAGWLP
                     LRLADFD"
     gene            complement(8879..9580)
                     /locus_tag="HRD51_RS11850"
                     /old_locus_tag="HRD51_11635"
     CDS             complement(8879..9580)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018536871.1"
                     /locus_tag="HRD51_RS11850"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HRD51_11635"
                     /product="DsbA family oxidoreductase"
                     /protein_id="WP_205364127.1"
                     /transl_table=11
                     /translation="MRVEIWSDIACPWCYIGKARFEAGLAAFAQRADVEVVHRSFELDP
                     AAPPATDVPILDMLATKYGVSREQAEAMEARVAEAAAGEGLGYRSDRIHGNTFDLHRLL
                     HLATAHGVQDALLTALYHANFAEARQLADPAVLTGIAVGAGVPEAEAARVLADPDAYAD
                     EVRADERAAAELGATGVPFFVIDRRYGVSGAQPADVFRQALERAHADSAVQVLAVAADD
                     ADGCGDGSCAV"
     misc_feature    10181..10195
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 20.15"
                     /tool="antismash"
ORIGIN
        1 gccgtccggg accggacgac caccgaccgc ttcgtggccg ccctggcgga cgtgctcggc
       61 gacccggccc ggtagagcgg acggggtgcg ccgcgcgggc ccacgcggcg cacccgtccc
      121 caccagcgcc cggcgccccc cacggcaccg atgcgctggg gtgcctcccc cgccgtgcgg
      181 acgaggggag ggtccgtcag ccgcgcccgt ccggcgcggc gctccggcgg cgggtgtaga
      241 ccatcgcccc ggcgccgccg gcgaccagca ggaccgcgcc gccgacgagg tacggggtgg
      301 cggcgctgcc gccggtctcg gcgaggttct cggtggccgg gcgggcgccg cccccggtgt
      361 gcgcctgggt gccggtgccc ggctcgtggg gctgaccagg gtgcgccggg gtgccgggct
      421 tgtccggggt gccgtgcggg gtggtgcagg tggcctgggc cagcgtgacc tggccgtgga
      481 cctccgcgac gttgagcttc aacgggttga ccgagacccg gagttggagc gcggtggcgg
      541 cggcgctcgc cgaggtggtg ctcttctgcg acaggtcgag ccggacgtcg ccgacgccgg
      601 gcacgtcgac ctcggtggtc ccgccggccc ggagcgcgac ccgcttgccc agcaccacga
      661 cgtcgcccag cacctgggac tcggcccgcg gatgcgctcc ggccgcgcag acggcgcggg
      721 cggtgacctg ctggacctcg gccagcggca gcgccggcag gccggggagc tggaccttgg
      781 cccggacgag gtgggcgtgg ccctcggcgg tccgcccgtc ggcggtggcc cgggcggtgg
      841 cgacgtcggc ccgcagcagg ctgaccggcc ggccgccgtc gatgccgtcc agccggacgg
      901 tgagcgcggt cttgtcggcc gaggccggag cgtggacctc gttgaggacg gcccggacgg
      961 ggacgttcag gctcttgtcg agcaggttga cgtccagggc ggtgcgcagc accaccgccc
     1021 ccgaggtgcc gtggccacgg gcgtcgtcgc cggtggcgta cgcggcgggc gcggcggcga
     1081 gggcgacggc gggaccggcc gtcaggacgg cggcggccag tgcggcgccg atccgcccgg
     1141 tggtgccggc ggagccggcg gtggaacggg tggacacgtg tgtggaaccc ccacatgaga
     1201 gatggcgccg ccgaccgcgc caatggggga catcacgcgg gccgacggca tcgactcgca
     1261 catcgtgtac gcacggaggg tgaacggtta gccacgcgag gttagttcac tccaaagggt
     1321 ggttttcgcg cccttattcg attttccccg acccctccgc cccggcgggc ctcccccgcc
     1381 acagccgtac cgggccgcgg gggcccggca cggcccgccg gacggggtgc cgtaggtcgc
     1441 tcagcccacg acccggccgc gcagcaccac caggcgcggc gccgccagca cccggacgtc
     1501 ggcccgcggg tcggtctcgt agacgaccag gtccgcggag gcgccctcgg tgagcccggg
     1561 gcggccgagc cactcccggg cgccccaggt cgtcgccgag agggcggcct cgacgggcag
     1621 ccccgccttc atcagctcgg cgacctcgtc ggcgaccagg ccgtgcggca gcgagccgcc
     1681 ggcgtcggtg ccggcgaaga tcgggacgcc ggcgtcgtag gcggcccgca cggtgtcgta
     1741 gcgccgggcg tgcagccggc gcatgtggtc cgcccagcgc gggaacttcg tctcgccgcc
     1801 ggccgcgagc tgcgggaagg tcgcgatgtt gaccagcgtg gggacgatcg cgacccggcg
     1861 ctccgcgaag agcgggatgg tgtcctcggt caggccggtg gcgtgctcga tgcagtcgat
     1921 gccggcctcg accagcgggg cgagggactc ctcggcgaag cagtgggcgg tgacccgggc
     1981 gccgagccgg tgcgcctcgg cgatggcggc ggccacctcg ccgggcggcc agcaggcggc
     2041 caggtcaccg gtggagcggt cgatccagtc gccgacgagc ttgacccagc cgtcgccgcg
     2101 ccgggcctcc tgggcgacgt aggcgaccag gtcggccggc tcgatctcgt gcgcgtagtt
     2161 gcggatgtag cgccgggtgc gggcgatgtg ccggccggcg cggatgatcc gcggcaggtc
     2221 ggggcggtcg tcgatccagc gggtgtccga gggcgagccg gcgtcccgga tcaggagggt
     2281 gccggcgtcc cggtcggtga gcgcctgctt ctcgctggtg gcggcgtcca ccggtccgtg
     2341 cgcgtccagt ccgacgtggc agtgggcgtc gaccaggccc ggcagcaccc agccggtcac
     2401 ggtcctggca tcgcgtgcca gggccggccg ctcgaaggtg acccggccgt cgatgaccca
     2461 cagctcgtcg cggacgtcgt ccggcccgac gaggaccctc cccttgaggt gcagcaccgc
     2521 accttcgctc atggacgtca tgcacgcact gtatgcgggg gcccgagccg gcggccacgg
     2581 tgcgcgaagg gcccgcgccg gccccgctcc gggcggtttt cgcaaaaacc cgcagcgcgc
     2641 ccgggacaca tcgaagcgcg gtgcttcccg catctcaccg gaagcgcttg gcggaacttc
     2701 tcgcacgact tttgaggaag cgcccaggac gccttctacc ggcgaattcc ctattcctca
     2761 gaagagcagc ttcccgtatt cttatgcccg ctttcctcaa gctcaaacgc caagatttcg
     2821 ctagtaccga gcctcataat tcaggcactt cgcgggagcg ttacgaagct gtgactgact
     2881 acacagcgtg tccgtttcgc cccgaactat cggaccaaac cgtgacactt tgcaaccggt
     2941 tcgcgcgctc gtgcgccccc gcgtgataac acagatcagg ttctcacgta acccctgccc
     3001 gcgatgcaat ttgcttttcg cctcggtaaa tttaatttcc atgaccgccg cacaagcaga
     3061 ccatgccggt gcccgaagcg aaatcagaga aataccggag ggcttcaagc tcgacacccc
     3121 ccgggtggag gacggggccg cgatctggcg catcgcccgc gactcgaaga cgctggacct
     3181 gaactcctcc tacagctacc tcctgtggtg ccgcgacttc gccgccacct ccgccgtcgc
     3241 ccgcgacgcg acgggcgagc cggccgcgtt catcaccggc tatctgcgcc cggaccgccc
     3301 ggacaccctc gtcgtctggc aggtcgccgt cgatgcggcg caccgcgggc ggggcctggc
     3361 cgccgccctg ctggacgggc tgaccacccg tgccgtcgac gaactgggcg tccgccgcgt
     3421 ggagaccacg atctcgcccg gcaacaccgc ctccaaccgg ctgttcgcct ccttcgccga
     3481 gcggcactcg gtgccgatcg agcgcgagat gctcttcgac gcggcgctct tccccgagca
     3541 gggccacgag gcggaggtcc tgcacctcat aggcccgttc gaggcgccgc ccggcagcag
     3601 acgctgaccc tggccgcgcg cacggcgcca ccccccggac ttctccctcc ccgcactcat
     3661 ctcccaggag catgctgtga ccctcaccca gcccgatctg agcgtcttcg aaaccgtcga
     3721 gtcggaggtg cgcagctact gccgtggctg gcccaccgtc ttcgaccgcg cccagggcag
     3781 ccgcctgacc gacgaggacg gccacaccta cctggacttc ttcgccggtg ccggttcgct
     3841 caactacggg cacaacaacg cggtcctcaa acgcgccctg ctcgactaca tcgagcggga
     3901 cggcatcacc cacggcctgg acatgtccac cacggccaag cgggccttcc tggagtcgtt
     3961 ccagaacatc atcctgcggc cgcgcgacct gccctacaag gtcatgttcc cgggcccgac
     4021 gggcgccaac tccgtcgagg ccgcgctcaa gctggcccgc aaggtcaagg gccgcgagtc
     4081 gatcgtgtcc ttcaccaacg ccttccacgg catgtcgctg ggcgccctcg cggtcaccgg
     4141 caactccatg aagcgggccg gcgccggcat cccgctggtg cacggcaccc cgatgccgtt
     4201 cgacaactac ctcgacggcc agacgccgga cttcctgtgg ttcgagcggc tgctggagga
     4261 ccagggctcg ggcctgaaca agcccgcggc cgtgatcgtg gagaccgtgc agggcgaggg
     4321 cgggatcaac gtcgcccgca ccgagtggct gcgcgccctg gccgccctgt gccagcgcca
     4381 ggacatgctg ctgatcgtcg acgacatcca gatgggctgc ggccgtacgg gtgccttctt
     4441 ctccttcgag gaggcgggca tcgtgccgga catcgtcacc gtctccaagt ccatcagcgg
     4501 ctacggactg ccgatggcgc tcaccctgtt caagccggag ctggacatct gggagcccgg
     4561 cgagcacaac ggcaccttcc gcggcaacaa cccggcgttc gtcaccgcgg ccgccgccct
     4621 ggacacgtac tgggccgacg gccagatgga gaagcagacg ctggcccgcg gtgagatcgt
     4681 cgaggcacac ctgaaggcca tcgccgagga gcacccgggc accttcgccg agtaccgcgg
     4741 ccgcggtctg gtgtggggcc tggagtgcac cgacaagagc cttgccgaca agatcgcggg
     4801 gcgcgccttc gagctgggtc tgctgatcga gacctccggc ccgcagagcg aggtcgtcaa
     4861 actgctgccc gcgctgacga ccacccccga ggaactggac gagggcctgc ggatcctcgc
     4921 ccgcgcggtc cgcgactgcg cctgaccgca gccctgcgcc gacatccctc gccgaaccac
     4981 agaaaggcat ccgacacacc atgatcgtcc gctcgttcca ggagatcgaa ggcaccgacc
     5041 gccacgtcaa ggccaagtcc ggcacgtggg agagcaagcg catcgtgctc gccaaggagc
     5101 gggtcggctt ctcgctccat gagacggtcc tctacgccgg aaccgagacg tccatgtggt
     5161 acgccaacca catcgaggcc gtggtctgcg tcgagggcgt cgccgagctg accaacgacg
     5221 agaccggcga gaagcacctc atcacgcccg gcgtgatgta cctgctcgac gggcacgaga
     5281 agcacaccat gcggatcaag gaggacttcc gctgcctgtg cgtcttcaac ccgccggtca
     5341 ccggtcgcga ggaccacgac gagaacggcg tctacccgct gctcaccgag cccgaggcgg
     5401 gctgagcggc accgcaccgc cgcgggccgc cgcgatgcca tgagattccg tacgagagga
     5461 gaggaaggca ccaccatgac caccgcaccc gagcgcaccg ccgacctgta cccgacccgt
     5521 gggacctccg aggtcatcac cccgcggaag gacccggtgg tgtggtcgcg gcccggagtc
     5581 gaggggccct tcgccgctgc cgagctgagc gacttcgacc gtgacggctt ctgcgcgctg
     5641 ccggagctgc tcaccgcgga cgaggtcgcg gtgtaccgcg ccgagctgga ccggctggtg
     5701 ggcgacccgg cgatgcgcgc cgacccgcgc tcgatcgtcg agccgacgtc acaggccgtc
     5761 cgctcggtct tcgaagtcca ccggatcagc gaggtgttcg cgggactggt cgccgacccc
     5821 cgggtggtcg gccgggcccg gcagatcctc ggctcggacg tctacgtcca ccagtcgcgg
     5881 atcaacgtca agcccgggtt cggtgcctcc ggcttctact ggcactcgga cttcgagacc
     5941 tggcacgccg aggacgggct gccgcacatg cgcacggtgt cggtctcgat cgcgctgacc
     6001 gagaactacg acaccaacgg cggcctgatg atcatgcccg ggtcgcaccg gtacttcgtc
     6061 gggtgcgagg gcgccacccc gaaggacaac tacaagaagt cgctccagat gcaggacgcg
     6121 gggacgccgt cggacgaggc gctgacgacg atggcggacc ggcacggcat ccgcctgttc
     6181 accggccggg ccggctcggc gacctggttc gactgcaacg ccatgcacgg ctccggcgac
     6241 aacatcacgc cgtacccgcg cagcaacgtc ttcatcgtct tcaacagcgt ggagaacccc
     6301 gcggtggagc cgttcgcggc gccggtccgg cgcccggagt acatcggggc gcgcgccccg
     6361 ttcgttccgg tcaaataggg gacggccggt gctccggcac cgggcgggga agcggggagg
     6421 ccccgcgcgc ggccgccgca cggcaccact ggggtgcggt gcggcggccg ccgcgcgcgg
     6481 ggcctcgacc gcgtcacgac ggccggggct gacgctccgt cagctctcgt cgtccagcgc
     6541 ggggtagtcc agatagccgc ggtcgtcgcc accgtagaag ccgtcccggt ccggggtgtt
     6601 gaacgggccg ccggcggcca gtcggcgggg caggtcgggg ttggcgatga acagcgcgcc
     6661 gtacgccagc agatcggcct cgccgcgctc gacgagcggg agttcctcgg ggccggtggg
     6721 ccggccttcg gtggccgggt tgagcaggaa cgggccgtcg aaccgcttcc gcagccgggc
     6781 cagcagctcc aggtcggtca cgtcgcaggt gtgcagatag gccaggccca gcggggcgac
     6841 ggcctcgatc agcgcggtgt aggtggcctc gggcgcgggc tcgtcgatgt cgttgaacgg
     6901 gttgccgggc gagagccgca aaccggtgcg gtgcgcgccg accgcgccgg cgaccgcctt
     6961 gaccacctcg atggcgaacc gggcgcgggc ctcgtcggag ccgccccacg cgtcggtgcg
     7021 ccggttggcg ttgggggcca ggaactggtg gatcaggtag ccgttggcac cgtggatctc
     7081 cacgccgtcg aagccggcct cgatggcgtt gcgggccgcg gcggcgaaac cgtcgacggt
     7141 cgcgcggatc tgctcgtcgg tcagctcgcg cggggtgacg aagtccgtcg ggccgttctg
     7201 ggtgaagccc tggcccgccg ccttcaccgc ggacgggccg accggctcca gcccgtcggg
     7261 cagcagcacc gggtggccga tccggccgcc gtgccacagc tgggcgaaga tccggccgcc
     7321 ggcggcgtgc acggagtcgg tgacccggcg ccagccggcg acctggtccg cggtgtgcag
     7381 ccccggggtg agcgggtagc cgcggccgac cacctcggtc tgggtggcct cggtgatgat
     7441 caggccggcc gaggcccgct gggtgtagta ctcgaccatg gacggggtgg ggacaccctc
     7501 ggcggtggcg cggttacggg tcatcggcgc catgacgatg cggttggcga gcggtatacc
     7561 ggcgagctct atggggtcga acgcagtggt cactgacggc ctttcagcgg tttgtcatgg
     7621 ttcgtcaact cggctgccga gccgaaccat tggccactac ctcagcggtc gatcccggcc
     7681 ccgcattccc ctggggccgt attcccctcg ggccccgaac cgcccgtcaa ggaaccaaca
     7741 gggacagcgc ccggctcacg tcctcggtcg agttgtagag gtggaacgcc gcccggagca
     7801 gcccgccgcg gaccgagacc cgcaccccgg cccgggccag ccgcggttcg gcgtccgcca
     7861 gccccggggt ggagacgatc gcggacccgg gtgcggcccg cggggtgaag ccggcggcct
     7921 ccagcccggc ccggtactgc tgcgccagcg cgacgttgtg ggcgtggacg gtgtccacgc
     7981 ccagctccgc gagcagcccg agggagtgct cggcggccac gtaggagtag tgggcgtgcg
     8041 gctcgtcgta acggcgggcg gtcgccgccg gtcggtcgac cggaccgtag ttggactccc
     8101 ccgggtcctc cccggcgacc caccccgcgt gcgccggcac cggccacccc gggccgcccg
     8161 gctcccccgc tccccgccga agaccatgaa cgtcgtcccg cgcgggcaca gcagccactt
     8221 gaacgccccg cacaccacgt agtcgaagtc cgcgagccgc agcggcagcc agcccgccgc
     8281 ctgcgtgatg tcgacatacg tcagcgcccc gtgcgcccgg gcggcgtccc ggatggcggc
     8341 caggtcggcg atccggccgt ccagcgcgtg caccgcgctc accgcgacca gcgccgtccc
     8401 gggccgcacc gcgtcggcga gcgcctccca cggcaccgcc cgcaccgtgc acccggcgcg
     8461 cgccgccagc gggttcacca gggagctgaa gtccccctca gcgaccagca cttcgctgcc
     8521 ggccggcagc gagcccgcga cgaacgccga ctgcaccgcg accgaactgc ccagcgccac
     8581 ccgctcctcg ggcaccccca tcagtcgggc gaaggcaccg cgcacccggg cgcccgtccc
     8641 gaggtagtcg cgccccatcg tcccgtgcga cgccgactcc gccacggcgg cggtcagcgc
     8701 ggcggcgctc cgcgcgggca gcagcccgca ggacgccgag ttcagataga ccgtctcggg
     8761 cgcgaactcg tcgccgccca gtgccatgtg tcgctgcatg gccccactct gctcggtgac
     8821 cgccccggcg tccacgggaa tgcccgggcc gcgaagcggg ggcggaccgg ccggtgggtc
     8881 agaccgcgca gctcccgtcg ccgcagccgt ccgcgtcgtc ggcggcgacg gccagcacct
     8941 ggacggcgga gtcggcgtgc gcccgctcca gggcctggcg gaagacgtcg gcgggctggg
     9001 cgccggagac gccgtagcgg cggtcgatga cgaagaaggg gacgccggtg gcgcccagct
     9061 cggcggcggc gcgctcgtcg gcacggacct cgtcggcgta ggcgtcgggg tcggcgagga
     9121 cccgcgcggc ctcggcctcg ggcacgccgg ccccgaccgc gatcccggtc agcaccgcag
     9181 ggtccgcgag ctgccgggcc tcggcgaagt tggcgtggta gagggcggtg agcagcgcgt
     9241 cctggacgcc gtgcgccgtg gccaggtgga gcagccggtg caggtcgaag gtgttgccgt
     9301 ggatgcggtc ggagcggtag cccagcccct cgccggccgc cgcctcggcc acccgggcct
     9361 ccatcgcctc ggcctgttcc cggctcacgc cgtacttcgt cgcgagcatg tccaggatcg
     9421 ggacgtcggt ggccggcggg gcggccgggt ccagttcgaa ggaacggtgg acgacctcga
     9481 cgtccgcccg ctgggcgaag gccgccagac cggcctcgaa ccgggccttg ccgatgtagc
     9541 accaggggca ggcgatgtcc gaccagattt cgacgcgcat ggggatgact ctcgatgact
     9601 ttcctcgggg ggaccggccc gcggccgatc aggggtacga aggggtgcaa cggtgccggc
     9661 agcgggctta ttccggatgc ccgggcgtcc cgaacgggtc ggccgccgcg cgatgcgccg
     9721 gatgcgcgtc ggccgggcgg taccgtcccg ctatgagcac gccaccaccg cacgcgaccg
     9781 acgggttctg ggagtcgacc ggggccacca agacgttcac gcacccgctc gacgccgagc
     9841 tgctgacgga gttcgtgccg cggaccgcgc gggtgctgga ctacggctgc gggtacgggc
     9901 ggctcaccgc cgaactggtc gggtcggggt accgggcggt gcgcggggtg gaccggtccg
     9961 cggcgctgat cgcgcggggg cggcgggagc atccggggct ggccctgatg cgctgggccg
    10021 ggttcccgct gccgttcgcg gaccgcagct tcgacgcggc gctgctgttc gcggtgctga
    10081 cctgtgtgcc ggacgacgcc ggccagcggg cgatcgtcgg ggagctgggg cggctggtgc
    10141 ggcccggtgg ggtgctctac gtgagcgatg tgccactgca ggacgacgcg ttccaccgcg
    10201 ggcagtacga gcggttcgcg gcgcggtacg ggacgtacgg ggtgttcgag acggaggacg
    10261 gcggggtgtt ccggcaccat ccgccggagt ggctgcgggg gctgctgcgg gaggcgggtt
    10321 tcacggtgct gcgggagcgc cgcacggtgg tgccgacgct ggacggccgc accgccgagc
    10381 ggctgcagat catggcccgg cggga
//
