LOCUS       NZ_CP054019            10216 bp    DNA     linear   CON 28-JAN-2024
DEFINITION  Streptomyces sp. A1-5 chromosome.
ACCESSION   NZ_CP054019
VERSION     NZ_CP054019.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. A1-5
  ORGANISM  Streptomyces sp. A1-5
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 9459690)
  AUTHORS   Dashti,Y., Sumang,F.A.M., de los Santos,E.L.C., Alcantara,E.P. and
            Challis,G.L.
  TITLE     Structural revision and biosynthesis of polyketides FXJ15321 and
            FXJ15322 from Streptomyces sp. A1-5
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9459690)
  AUTHORS   de los Santos,E.L.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2020) Warwick Integrative Synthetic Biology
            Centre, University of Warwick, Gibbet Hill Road, Coventry CV4 7AL,
            United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: JUN-2019
            Assembly Method                   :: Flye v. 2.4.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Oxford Nanopore MiniION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/28/2024 04:28:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,328
            CDSs (total)                      :: 8,230
            Genes (coding)                    :: 7,847
            CDSs (with protein)               :: 7,847
            Genes (RNA)                       :: 98
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 156 of 383
            Pseudo Genes (incomplete)         :: 280 of 383
            Pseudo Genes (internal stop)      :: 26 of 383
            Pseudo Genes (multiple problems)  :: 73 of 383
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 22:08:01
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1698469
            Orig. end                         :: 1708685
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP054019.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Philippine National
            Collection of Microorganisms.
FEATURES             Location/Qualifiers
     region          1..10216
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="11"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10216
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10216
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5216](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5216)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(735..749)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 17.3"
                     /tool="antismash"
     gene            complement(1687..3966)
                     /locus_tag="HRD51_RS07585"
                     /old_locus_tag="HRD51_07410"
     CDS             complement(1687..3966)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018540973.1"
                     /locus_tag="HRD51_RS07585"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HRD51_07410"
                     /product="NHLP family bacteriocin export ABC transporter
                     peptidase/permease/ATPase subunit"
                     /protein_id="WP_235092651.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 5.8e-38, bitscore:
                     128.6, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTAPDAAAAPPQHLPPAGRGRHRPEGAPPTRRRRPPATAPKAKKS
                     VRTPTVLQMEAVECGAASLAMVLAHYGRHVPLEELRIACGVSRDGSRASNLLKAARSYG
                     LSAKGMQMEPAALAEVRAPAILFWEFNHYVVYDGMGRRLGRRGVHINDPDKGRRFVPTE
                     DFDTSFTGVALVFEPTEAFRKGGRRPSVRSALPARLRGTAGTLLAALLASLLLVGVGAA
                     LPALSRTYIDMFLIGNQTSLLGPLFAAMSAMVVLTAVLTGLQQANLLRGRIISSTLSSA
                     RFLRHLLRLPVTFFSQRSPADLVQRLQSNDAVAETLARDLAAAAVDGVVVVLYAVLLWT
                     YDPQLTVIGVLIALLNVVAMRIVVRLRATHTQKLRADTARLTNTSYTGLQLIETMKATG
                     GENGYFRRWAGQHATTLEEQQRLGVPSAWLAVVAPTLATLNSALILWIGGLRAVEGHIS
                     IGLLVAFQALVTRFTAPITRLNGVAGRIQDFAADVARLKDVESFPADAHYSRPDADAGT
                     RRLKGHITLDGVTFGYSPLDAPLLTGFSLAVGPGRQVALVGGSGSGKSTVSRLISGLYT
                     PWEGTIRIDGQRLADIPRSALAASVSFVDQDVFLFEGTVRDNVALWDPSIPDDAVIAAL
                     QDAALYDDVIARRPDGIHSRVEQDGRNFSGGQRQRLEIARALVRRPSILVLDEVTSALD
                     AGTEAVIMDNLRRRGCACVIIAHRLSTVRDSDEIVVLDHGTVVERGRHEELVAAGGAYA
                     ELVKEH"
     gene            complement(3963..4769)
                     /locus_tag="HRD51_RS07590"
                     /old_locus_tag="HRD51_07415"
     CDS             complement(3963..4769)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1029: RND family
                     efflux transporter MFP subunit"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018540972.1"
                     /locus_tag="HRD51_RS07590"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HRD51_07415"
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="WP_235092652.1"
                     /transl_table=11
                     /translation="MQFRQQALSKLQSPEELDLPVRFARPQGWLVLTVTVLVMIAATVW
                     AVTGTVSATLTAPGILTHGRGSYVLQSPVAGQVTAVLAEEGQPVAAGAPLARIRTVQGT
                     TTVVRALAAGRLTSLVATIGEVVTTGADLAAVERTNGPHDPLTATLYVPAASAAALPAG
                     AAVDLTVASAPTQRFGVLRGHVAAIGRTAQSRQRISAFLGSSQLGEQFSRAGQPVAVLV
                     RLDTASTATGYAWSTSDGPPFALDSMTQATAAVHLAAQHPIDWLLS"
     misc_feature    4734..4746
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 16.37"
                     /tool="antismash"
     gene            complement(5001..5216)
                     /locus_tag="HRD51_RS07595"
                     /old_locus_tag="HRD51_07420"
     CDS             complement(5001..5216)
                     /GO_component="GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /GO_process="GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: strepbact"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016576071.1"
                     /locus_tag="HRD51_RS07595"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HRD51_07420"
                     /product="type A2 lanthipeptide"
                     /protein_id="WP_205362510.1"
                     /sec_met_domain="strepbact (E-value: 2.4e-21, bitscore:
                     74.9, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNNAQVQTQEISDADLDNVSGGLVGGVLSTVTTTVGSVAPVGSVL
                     ATTTGLVQQTTGLDVNGIVGGATAGL"
     misc_feature    complement(5234..5248)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 14.56"
                     /tool="antismash"
     misc_feature    5235..5248
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 12.98"
                     /tool="antismash"
     misc_feature    complement(5235..5249)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 16.62"
                     /tool="antismash"
     misc_feature    5236..5249
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 16.16"
                     /tool="antismash"
     misc_feature    complement(5588..5606)
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 18.26"
                     /tool="antismash"
     misc_feature    5690..5707
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 15.91"
                     /tool="antismash"
     misc_feature    5699..5715
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 15.68"
                     /tool="antismash"
     misc_feature    complement(5709..5723)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 16.67"
                     /tool="antismash"
     gene            5894..6934
                     /locus_tag="HRD51_RS07600"
                     /old_locus_tag="HRD51_07425"
     CDS             5894..6934
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018540970.1"
                     /locus_tag="HRD51_RS07600"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HRD51_07425"
                     /product="PHP domain-containing protein"
                     /protein_id="WP_235092653.1"
                     /transl_table=11
                     /translation="MDPVAALERIAFLLERQGAVTYRVQAFRTAASVLDGLAAEELRER
                     AGNGTLGRLKGLGPKTTRVVEEALAGQQPAYLTRLTEEAGGPLVEGERGLALRRALRGD
                     CHLHSDWSDGGSPVEAMARAARDLGHDWCALTDHSPRLTVAHGLTAERLRRQLDVVAEV
                     NARLAPFRLLTGIECDILDDGSLDQEPELLDRLDVVVASVHSTLRMDRRPMTRRMLAAV
                     GNPLVDVLGHCTGRRLTGRLRPESTFDAAEVFAACAEHRTAVEINCRPDRFDPPRRLLR
                     QALDAGALFAVDSDAHAPGQLDWQIYGCARAEECSVPADRIITTWTARQLTTWTRTGRT
                     PHGAGG"
     gene            complement(6977..7885)
                     /locus_tag="HRD51_RS07605"
                     /old_locus_tag="HRD51_07430"
     CDS             complement(6977..7885)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1014: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018091736.1"
                     /locus_tag="HRD51_RS07605"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HRD51_07430"
                     /product="LysR family transcriptional regulator"
                     /protein_id="WP_205362513.1"
                     /transl_table=11
                     /translation="MIEARRLHILRALADHRTVTAAAAALYLTPSAVSQQLAALEQETG
                     HRLVERGARGVRLTPAGDILLGHANAVLAQLERAEAELAAYDKGEAGTVTVAAFATGIA
                     LVLAPALAELAGSAPGIRVRVQDAEGDASLLMVLDRQVDVAVAVEYRGAPDADDPRLTR
                     VPLYAEPFDAVLPPGHPLAGAAAVPLAGLAKDPWIGPYSGNPCHDVVVLACEYAGFRPR
                     LTHSSDDFRAVVALASAGVGVALVPRSALRGMDLGGVVVRPVDGAPPTRRVFAAVRRGA
                     EDHPLFRPVLAALRTAASAQG"
     gene            complement(7937..9130)
                     /locus_tag="HRD51_RS07610"
                     /old_locus_tag="HRD51_07435"
     CDS             complement(7937..9130)
                     /EC_number="2.3.1.29"
                     /GO_function="GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA]"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_process="GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Aminotran_1_2 (45-388). E-value:
                     1.7e-71. Score: 233.2. Matches aSDomain:
                     nrpspksdomains_HRD51_RS07610_Aminotran_1_2.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_1_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1109: 8-amino-7-oxononanoate synthase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012999157.1"
                     /locus_tag="HRD51_RS07610"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HRD51_07435"
                     /product="glycine C-acetyltransferase"
                     /protein_id="WP_235092654.1"
                     /sec_met_domain="Aminotran_1_2 (E-value: 2.6e-69, bitscore:
                     233.0, seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFDAVRDDLRTALDEIRAAGLHKPERVIGTPQSATVAVTAGGRPG
                     EVLNFCANNYLGLADHPEVIAAAHGALDRWGYGLASVRFICGTQEVHKELERRISGFLG
                     QEDTILYSSCFDANGGVFETLLGPEDAVISDALNHASIIDGIRLSKARRLRYANRDMDD
                     LERQLKDAEGARRTLIVTDGVFSMDGYLAPLREICDLADRYGAMVMVDDSHAVGFVGPG
                     GRGTPELCGVMDRVDIITGTLGKALGGASGGYVAARAEIVALLRQRSRPYLFSNSLAPV
                     IAAASLKVLDLLESAGDLRERLNANTALFRSRMTEEGFAILPGEHPIAPVMIGDAAEAA
                     RMAELLLERGVYVIGFSYPVVPKGEARIRVQLSAAHSTEDVHRAVDAFVAARAALAG"
     aSDomain        complement(7967..8995)
                     /aSDomain="Aminotran_1_2"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_HRD51_RS07610_Aminotran_1_2.1"
                     /evalue="1.70E-71"
                     /label="HRD51_RS07610_Aminotran_1_2.1"
                     /locus_tag="HRD51_RS07610"
                     /protein_end="388"
                     /protein_start="45"
                     /score="233.2"
                     /tool="antismash"
                     /translation="EVLNFCANNYLGLADHPEVIAAAHGALDRWGYGLASVRFICGTQE
                     VHKELERRISGFLGQEDTILYSSCFDANGGVFETLLGPEDAVISDALNHASIIDGIRLS
                     KARRLRYANRDMDDLERQLKDAEGARRTLIVTDGVFSMDGYLAPLREICDLADRYGAMV
                     MVDDSHAVGFVGPGGRGTPELCGVMDRVDIITGTLGKALGGASGGYVAARAEIVALLRQ
                     RSRPYLFSNSLAPVIAAASLKVLDLLESAGDLRERLNANTALFRSRMTEEGFAILPGEH
                     PIAPVMIGDAAEAARMAELLLERGVYVIGFSYPVVPKGEARIRVQLSAAHSTEDVHRAV
                     DAF"
     misc_feature    complement(9329..9338)
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.16"
                     /tool="antismash"
     misc_feature    9394..9407
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 18.47"
                     /tool="antismash"
ORIGIN
        1 agcgcggcga gcgccagcgg cacgctgtag aggagcagca tcacgaggtt gaccgcgccg
       61 accgtggcgg cctgcacggc caccggcccg acgccggcca gcacctggcg gatggcgctg
      121 atgcccatgg cggcactggc cagttcaccg gtggagcggc cggcgaagaa cttcgtcggc
      181 aggcgcagca gccggtccca gacggccggc tggaggacgc tctcgatccg gccctcgatg
      241 cgcagcacgg tgaggttctg cagcagggtg aaggccgccg cgaccacgcc ggcgatgatg
      301 acggcgagcg cgacctggac gatcgggccg cgggcggcgg ccggtacgta gacgccgagg
      361 accttgccgg tggcgagcgg gacgagggcg ccgagggcga ccgtcaccag tccgctcagg
      421 gcgaggttgc gcaggtcggc gcggttcccg cggaggctga agcgcatcag ccgccacagc
      481 gtgagccggc gttcgggcag cggccggtag aacatcaccg cgtgcggctc gaactcgtcg
      541 gcggtgtcct tgccgatccg gatccgcagc ccggaggcgg ggttgaccgc ctcgtaccgg
      601 ccgcgccgcc acaacagcgc caccggggca ccggacttgg cgcggtggcc caccaggggc
      661 ccggcgtcgg tgcgccacca gcggccgtcg aggcggaccg cgcgggtgcg gatccgggag
      721 gcgagggcga tccgttcgac ggggctgatc cggtcgccgg cggcggcgcc cttgggcggt
      781 tcggtgagca cgatgccggc ggccccggcg accttccggc agaccgcgaa ggtcaggtcg
      841 tcgccggccc ggtcgcggcc cgcggcggcg cgtccggagc ggtccttgcc gatggacgcc
      901 agcagtgcct ggtcggcgcg ttcccggacg gcctcaccgg ccttgatacc ggccgccgcg
      961 cggtcctcgt gggcccgctc cagctgctcg atccagcggt cgacggcgga cagcagtcgg
     1021 tactgctggt tgaccatccg ctgccacagc gccgcgtcga cgagcaggtc ggcggcggcc
     1081 tcggcgctgt aggaggcgcc gtactggacg ctgccgggcg gcaccggcat ccacaggacg
     1141 tcgtcgtcgg cgaccgcttc gtcgctgggc cggccgtcca gcggcgcctc gaacagcacg
     1201 cccagaccgc gcgcggtgcc gagcgcgaag gcgtgctcca ggggggtggc cggctgcccg
     1261 ccgtaggtgt cgtcgggtcc gaaggtgccc tcgtgcccgt acgcgccctg gtcgccgtgg
     1321 tcggcgtggg gcagttcgcg cagggcgatc cggcgcagac cgcagtgctg ggaggggcgg
     1381 ccgacgaggg tgtgctgggg gccttggacg ggaccgagca gcagggtgcc ggcttcgagg
     1441 cggccgagga agtgccagtg gccttcctgg ccggcgtcca ccgcgaacag gtcgagggtg
     1501 ccgccggtga ccagccacag cacgtgcggg ccttcgaggg ggacgtcgcg cagcccggcg
     1561 cagtcgacgg gggtgccgag gccgcccagg gcgtgcgtga ccgcgtcgac gccgtgcgga
     1621 tccgcggccg gtgcggccgg ctgcgtggcg gatcccgcat ccggggacgg gtgtacggat
     1681 gccacgtcag tgctccttga ccagctcggc gtaggcgccc ccggcggcga ccagctcctc
     1741 gtgccggccg cgttcgacga ccgtgccgtg gtcgagcacc acgatctcgt cgctgtcgcg
     1801 gacggtgctc agccggtggg cgatgatgac gcaggcgcag ccgcggcgcc gcaggttgtc
     1861 catgatgacc gcctcggtcc cggcgtccag ggcgctggtg acctcgtcga ggacgaggat
     1921 gctggggcgc cggaccagcg cccgggcgat ctccaggcgc tgccgctggc cgccggagaa
     1981 gttgcggccg tcctgttcga cgcggctgtg gatgccgtcg gggcggcggg cgatgacgtc
     2041 gtcgtagagg gcggcgtcct ggagggcggc gatcaccgcg tcgtcgggga tggaggggtc
     2101 ccacagcgcg acgttgtccc ggacggtgcc ctcgaagagg aagacgtcct ggtcgacgaa
     2161 ggagaccgag gcggccagcg cgctgcgcgg gatgtccgcg aggcgctgtc cgtcgatccg
     2221 gatcgtgccc tcccaggggg tgtagagacc ggagatcagc cgggagacgg tggacttgcc
     2281 gctgcccgag ccgccgacca gggcgacctg gcggccgggg ccgacggcca gggagaagcc
     2341 ggtcagcagc ggggcgtcga gcgggctgta gccgaaggtg acgccgtcga gggtgatgtg
     2401 gcccttgagg cggcgggtgc cggcgtccgc gtccgggcgg gagtagtggg cgtcggcggg
     2461 gaagctctcg acgtccttga ggcgggccac gtcggcggcg aagtcctgta tgcggccggc
     2521 gacgccgttg agccgggtga tcggtgcggt gaagcgggtc accagggcct ggaaggcgac
     2581 cagcaggccg atggagatgt ggccctcgac ggcgcgcagg ccgccgatcc acaggatcag
     2641 tgcgctgttg agggtggcaa gggtgggggc gacgaccgcc agccaggcac tggggacgcc
     2701 gagccgctgc tgctcctcca gcgtggtggc gtgctggccg gcccagcggc ggaagtagcc
     2761 gttctcgcca ccggtggcct tcatggtctc gatgagttgc aggccggtgt aggaggtgtt
     2821 ggtcaggcgg gcggtgtcgg cgcgcagctt ctgggtgtgg gtggcgcgca gccggaccac
     2881 gatccgcatg gcgacgacgt tgagcagcgc gatcagcacg ccgatgacgg tgagttgggg
     2941 gtcgtaggtc cacagcagca ccgcgtagag gacgaccacc acgccgtcga cggcggccgc
     3001 ggccaggtcg cgggcgaggg tctcggcgac cgcgtcgttg gactgcaggc gctggacgag
     3061 gtcggcgggc gagcgctggg agaagaaggt gaccggtagc cgcagcagat ggcgcaggaa
     3121 gcgggcgctg ctgagggtgg aggagatgat ccggccgcgg agcaggttgg cctgctggag
     3181 gccggtcagg acggcggtga gcaccaccat cgcgctcatc gcggcgaaca gcggtcccag
     3241 cagcgaggtc tggttgccga tcaggaacat gtcgatgtag gtgcggctga gcgcgggcag
     3301 cgcggcgccg acgccgacca gcagcaggct ggcgagcagc gccgccagca gggtgccggc
     3361 ggtgccgcgc agccgggccg gcagggcgct ccgcacgctg ggccggcggc cgcccttgcg
     3421 gaacgcctcg gtgggctcga agaccagggc gacgccggtg aagctggtgt cgaagtcctc
     3481 cgtcggcacg aagcggcggc ccttgtcggg gtcgttgatg tggacgcccc ggcggccgag
     3541 gcggcggccc atgccgtcgt agacgacgta gtggttgaac tcccagaaga ggatggccgg
     3601 cgcccggacc tcggcgaggg cggccggctc catctgcatg cccttggccg acaggccgta
     3661 actgcgggcg gccttgagga ggttgctggc ccgggagccg tcgcgggaga cgccgcaggc
     3721 gatccgcagt tcctccagcg gcacatggcg tccgtagtgg gcgagcacca tcgccaggga
     3781 ggcggcgccg cactccaccg cttccatctg gagcacggtg ggggtgcgca cggacttctt
     3841 cgccttgggg gcggtggccg gcggtctgcg gcggcgggtg ggtggcgcgc cctcggggcg
     3901 gtgccgtccg cggccggcgg gcgggaggtg ctgcgggggt gccgcggcgg cgtcgggtgc
     3961 ggtcacgaga gcagccaatc gatcgggtgc tgcgcggcca ggtggacggc ggcggtggcc
     4021 tgggtcatcg agtccagggc gaacggcggg ccgtcggagg tggaccaggc atagccggtg
     4081 gcggtggacg cggtgtcgag ccggaccagc accgcgaccg gctggccggc gcgggagaac
     4141 tgctcgccga gttggctgct gccgaggaac gcggagatcc gctggcggct ctgggcggtg
     4201 cggccgatgg ccgcgacgtg gccgcgcagc acgccgaacc gctgggtggg ggcggaggcg
     4261 acggtgaggt ccacggcggc gccggcgggg agggcggcgg cgctcgcggc cgggacgtag
     4321 agggtggccg tcagggggtc gtgggggccg ttggtccgct ccacggcggc gaggtcggcg
     4381 ccggtggtga cgacctcgcc gatggtggcg accaggctgg tcagccggcc ggcggcgagc
     4441 gcgcggacga cggtggtggt gccctgcacg gtccgtattc gcgccaacgg ggcgcctgcg
     4501 gcgaccggtt ggccctcctc ggcgaggacc gcggtgacct ggccggcgac cgggctctgc
     4561 aggacgtagc tgccgcggcc gtgggtgagg atgccggggg cggtgagggt ggcggagacg
     4621 gtgccggtga ccgcccagac ggtcgcggcg atcatgacga ggacggtgac ggtcagcacc
     4681 agccagccct gggggcgggc gaagcgcacc ggcaggtcga gctcctcggg cgattgcagc
     4741 ttggaaaggg cctgttggcg gaactgcacg acactcttcc ctcaactgca ggtgctggta
     4801 cgggagtagc gggagtgtac cgccgccgga gatccggtgg cggtttcccg gcggcgaaca
     4861 gccgggtgcc ccggacaacc cggccggaac cgggttgtcc ggggcactcg gtacggcaga
     4921 tcggcaggga gggggcgccg actgccgtca cgaccgttcg tcgccgcctg tgccgcgcgg
     4981 cggcacagcg gcggctcgga tcagagaccg gcggtcgcgc cgcccacgat gccgttgacg
     5041 tcgaggccgg tggtctgctg gaccaggccg gtggtggtgg cgaggacgct gccgaccggg
     5101 gcgaccgagc ccacggtggt ggtgacggtg ctcagcacgc cgccgacgag gccaccggac
     5161 acgttgtcca ggtcggcgtc ggagatttcc tgggtctgca cctgggcgtt gttcatgatg
     5221 tttcccttcg gatcaatatc aagaaaaaac aagcgaaacg ggccgtccgg tgggtgaccc
     5281 ggccgcggcc acggcagtcc ggcccccgat atcgggaaac cccggcgact tccgcggtgc
     5341 gatggatcaa agcacgcggg cgggtttccc tgctaatcgg atgagcggtg atccggacgc
     5401 gaccgcgccg cgaaattacc ggacgtgtac gtgcgtccat ggcttgatcg aagcatcttc
     5461 acatctcctc cggtttcttt tccgtcgaag gggcgttgcg acactcccgg aatcgggcat
     5521 tccggaacta gatcgccgcc caccagggcg gccgtgtcac ccgaaacccg gaagcgtgtg
     5581 ccggctgcgc attcggtgtg cagatttcct ggaggtcacc gaacttaacg tccggctgac
     5641 gcaccggccc gccgagaagt ccgattcgcg tttccgcagc acggccggga tgttgcccag
     5701 gttttaaccg aacatgattg ccctagactt ccgatgcact aatgaccgga atcatcacgg
     5761 ctatcgccca tgtcattccg atggaaacgg atcgagcatc cagagaacgg acatattcgc
     5821 ataactgcgg tagggggcat tcgccggccc gaccgcggtg gcagccgcgc cccgcccggt
     5881 gcacggtggg gaggtggatc ccgtggcggc gctggagcgg atcgcgttcc tgctggagcg
     5941 gcagggggcg gtgacgtacc gggtgcaggc gttccgcacg gcggcgtcgg tgctcgacgg
     6001 actggccgcg gaggaactgc gcgagcgcgc cgggaacggg acgctgggcc gtctgaaggg
     6061 gctcggcccc aagacgaccc gggtcgtcga ggaggcactg gccgggcagc aacccgccta
     6121 tctgacgcgg ctgacggagg aggccggcgg cccgctggtc gagggggaac gggggctggc
     6181 gctgcggcgg gccctgcgcg gcgactgcca tctgcactcg gactggtcgg acggtggcag
     6241 ccccgtcgag gcgatggcca gggccgcccg cgacctcggc cacgactggt gcgcgctgac
     6301 cgaccactcc ccccggctca ccgtcgccca cgggctgacc gccgagcggt tgcggcggca
     6361 gttggacgtg gtcgccgagg tcaacgcgcg cctggcgccg ttccggctgc tgaccgggat
     6421 cgagtgcgac atcctcgacg acggctcgct ggaccaggag ccggagctgc tggaccggct
     6481 cgacgtcgtg gtcgcctcgg tccactccac actgcggatg gaccggcggc cgatgacccg
     6541 gcggatgctg gccgcggtcg gcaatccgct ggtggacgtc ctcggccact gcaccggccg
     6601 acggctcacc ggccggctcc gcccggagtc gacgttcgac gccgcggagg tcttcgcggc
     6661 ctgcgccgag caccgcaccg cggtggagat caactgccgc ccggaccgct tcgacccgcc
     6721 gcgccgcctg ctgcggcagg ccctggacgc cggcgccctc ttcgccgtcg acagcgacgc
     6781 gcacgcgccc ggccagctgg actggcagat ctacggctgc gcccgcgccg aggagtgcag
     6841 cgtcccggca gaccggatca tcaccacctg gacggcccgc cagctgacga cctggacgcg
     6901 gaccggccgc accccgcacg gggccggcgg ctgaccgtcc ggggccggtc cgggccccgg
     6961 tccgggacca ctcgcctcag ccctgcgcgg acgccgccgt gcgcagcgcc gccaacaccg
     7021 ggcggaacag cggatggtcc tccgcgccac ggcggaccgc ggcgaagacc cggcgggtgg
     7081 gcggggcgcc gtcgaccggt cgcaccacca cacccccgag gtccatcccg cgcagcgccg
     7141 agcgtggcac cagggccacc ccgacgcccg ccgaggccag cgccaccacc gcccggaagt
     7201 cgtccgagga gtgcgtcagg cgcggccgga acccggcgta ctcgcaggcc agcacgacca
     7261 cgtcatggca gggattgccg gaatagggcc cgatccaggg gtccttggcg agcccggcca
     7321 gcggcaccgc cgccgccccg gccagcggat gcccgggcgg cagcaccgcg tcgaacggct
     7381 cggcgtagag cgggacccgg gtcagccggg ggtcgtccgc gtccggcgcg ccccggtact
     7441 cgacggccac cgccacgtcg acctgccggt ccagcaccat cagcaggctg gcgtcgccct
     7501 ccgcgtcctg gacccggacc cggatgccgg gtgcggaacc ggcgagttcg gccagggccg
     7561 gggcgagcac cagcgcgatg ccggtcgcga acgccgcgac cgtcaccgtg ccggcctccc
     7621 ccttgtcgta cgccgccagc tccgcctcgg cgcgctccag ctgggccagc acggcgttgg
     7681 cgtgaccgag caggatgtcg cccgcggggg tcagccggac gccgcgggcg ccgcgctcga
     7741 cgaggcggtg gccggtctcc tgctccaggg cggccagttg ctgggagacc gccgagggcg
     7801 tgaggtagag cgcggcggcc gccgccgtca ccgtgcgatg gtcggccagc gcacgcagga
     7861 tgtgcagccg acgggcttcg atcatggttc cagtttcgca gggttcggcg gggttctgtg
     7921 ccccggaacc ccgcggtcag cccgccaggg cggcgcgggc ggccacgaag gcgtccaccg
     7981 cgcggtgcac gtcctcggtg gagtgggccg cggacagctg gacgcggatc cgggcctccc
     8041 ccttggggac caccgggtac gagaagccga tgacgtacac cccgcgctcc agcagcagct
     8101 cggccatccg ggccgcctcg gccgcgtcgc cgatcatcac cggcgcgatc gggtgctcgc
     8161 cgggcaggat cgcaaagccc tcctcggtca tccgtgagcg gaacagcgcg gtgttggcgt
     8221 tgaggcgttc gcgcaggtca ccggcggact ccaggaggtc gagcaccttc agcgaggcgg
     8281 ccgcgatcac cggcgcgagc gagttggaga agaggtaggg gcgggagcgc tggcggagca
     8341 gggcgacgat ctcggcgcgc gccgcgacat agccgccgga ggcgccgccc agcgccttgc
     8401 cgagggtgcc ggtgatgatg tcgacgcggt ccatcacgcc gcacagctcg ggggtgccgc
     8461 gaccgccggg gccgacgaag ccgaccgcgt gcgagtcgtc gaccatcacc atcgcgccgt
     8521 agcggtcggc caggtcgcag atctcccgca gcggcgcgag gtagccgtcc atcgagaaca
     8581 cgccgtcggt gacgatgagg gtgcgccggg cgccctcggc gtccttcaac tgccgttcca
     8641 ggtcgtccat gtcgcggttg gcgtaacgca gccggcgggc cttggagagc cggatgccgt
     8701 cgatgatgga ggcgtggttg agggcgtcgg agatgaccgc gtcctccggg ccgagcaggg
     8761 tctcgaagac accgccgttg gcgtcgaagc aggaggagta gaggatggtg tcctcctggc
     8821 cgaggaagcc ggagatccgc cgctccagct ccttgtgcac ctcctgggtg ccgcagatga
     8881 agcggaccga ggccaggccg tagccccagc ggtccagggc cccgtgggcg gcggcgatga
     8941 cctcggggtg gtcggccagg cccaggtagt tgttggcgca gaagttgagg acctcgccgg
     9001 ggcggccccc ggcggtgacc gcgacggtgg cggactgcgg ggtgccgatg acccgctcgg
     9061 gcttgtgcag tccggcggcg cggatctcgt cgagggcggt gcgcagatcg tcgcgtacgg
     9121 cgtcgaacat ggggtggtgt ctccttaggc ggggtcgagg tcgggcggcg ggagggcagg
     9181 ccggctggcg gcgccgtcat cgggggtggc ggggctcagg cgctccagtc gaggatgacc
     9241 ttgccgctct ggccgctggc cgcgtcggcg aaggcggtct cgaactcctg gtacgggtag
     9301 cggccggtga tcacgggggc gaggtcgagg ccgccttcca gcagcaccga catcgcgtac
     9361 caggtctcga acatctcccg gccgtagatg cccttgatcg tgatcatgga ggtgacgatc
     9421 cgggaccagt cgaccgggaa ctcctcggcg gccaggccca gcacggcgat ccggccgccg
     9481 tgcgtcatgt tgccgatcat gtcgcgcatg gcctcgggcc ggccggacat ctccaggccg
     9541 acgtcgaagc cctcgcgcag gcccagttcg cgctggccgg cctcgatcga ggtgtgggcg
     9601 acgttgaggg cgaggctgac gccgaccttg cgggccagtt ccaggcgctg ctcgctgacg
     9661 tcggtgatca tcacatggcg ggcgccggcg tgccgggcga ccgccgccgc catgatgccg
     9721 atcggtccgg cgccggtgat caggacgtcc tcgccgacca gcgggaagga gagcgcggtg
     9781 tgcacggcgt tgccgaacgg atcgaagacc gcggcgacgt cgaggtcgac cgggacccgg
     9841 tgcacccaca cgttggtggc gggcagcgcg acgtactcgg cgaaagcgcc gtcccggccg
     9901 acgcccaggc cgacggtggc gcggcacagg tggcggcggc cggcctggca gttgcggcag
     9961 ctgccgcaga ccaggtggcc ctcgccgctg accaggtcgc cgggcttgat gtcggccacg
    10021 tcccggccgg tctcgacgac ctccccgacg aactcgtggc cgacggtcag cggggtccgc
    10081 acggcctgct gcgcccagcc gtcccaggcc cggatgtgca ggtcggtgcc gcagatgccg
    10141 gtgcgcagca ccttgatcag cacatcgccg gggccgatcg tcggttccgg cacgtccgtc
    10201 agccagagtc ccggtt
//
