LOCUS       NZ_CP090412            10419 bp    DNA     circular CON 31-MAR-2024
DEFINITION  Ligilactobacillus salivarius strain 2102-15 plasmid pLS2102-15_1,
            complete sequence.
ACCESSION   NZ_CP090412
VERSION     NZ_CP090412.1
KEYWORDS    RefSeq.
SOURCE      Ligilactobacillus salivarius
  ORGANISM  Ligilactobacillus salivarius
            Bacteria; Bacillota; Bacilli; Lactobacillales; Lactobacillaceae;
            Ligilactobacillus.
REFERENCE   1  (bases 1 to 140826)
  AUTHORS   Karlyshev,A.V.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JAN-2022) Biomolecular Sciences, Kingston University
            London, United Kingdom, Penrhyn Road, Kingston upon Thames KT1 2EE,
            United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: OCT-2021
            Assembly Method                   :: Unicycler v. 0.4.0
            Assembly Name                     :: sequence.fsa
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80.0x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_021432185.1-RS_2024_03_31
            Annotation Date                   :: 03/31/2024 06:21:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 1,966
            Genes (coding)                    :: 1,939
            CDSs (with protein)               :: 1,939
            Genes (RNA)                       :: 104
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 10 of 27
            Pseudo Genes (incomplete)         :: 10 of 27
            Pseudo Genes (internal stop)      :: 12 of 27
            Pseudo Genes (multiple problems)  :: 4 of 27
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 23:32:12
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 69509
            Orig. end                         :: 79928
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP090412.1.
            Bacteria and source DNA available from Andrey Karlyshev, Kingston
            University London, United Kingdom.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10419
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10419
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10419
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5419](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5419
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            836..1102
                     /locus_tag="LZF92_RS09710"
                     /old_locus_tag="LZF92_09710"
     CDS             836..1102
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703461.1"
                     /locus_tag="LZF92_RS09710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LZF92_09710"
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="WP_041823201.1"
                     /transl_table=11
                     /translation="MDYLVIKAIIWIFFITSAILEVNYKKKYGELDKRARKQATLGTFL
                     IVTTIFDEGYENKILYIIVLIALLVYVVLVIYRLVMAIKDRKK"
     gene            1123..1464
                     /locus_tag="LZF92_RS09715"
                     /old_locus_tag="LZF92_09715"
     CDS             1123..1464
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706832.1"
                     /locus_tag="LZF92_RS09715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LZF92_09715"
                     /product="hypothetical protein"
                     /protein_id="WP_160943840.1"
                     /transl_table=11
                     /translation="MKAIYRGRKVDVWQVSRDNVQPDWVKDAFEHNGLRWLHGSGNKVR
                     IYWPAVTRAKKGIVRTILNDISASFVGIAGGSVSMYDIGFEGDVIDFTNSKVVTAEKFA
                     KKYRIVSED"
     gene            1578..2225
                     /locus_tag="LZF92_RS09720"
                     /old_locus_tag="LZF92_09720"
     CDS             1578..2225
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HAD_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1115: HAD-superfamily hydrolase, subfamily IA,
                     variant"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340246.1"
                     /locus_tag="LZF92_RS09720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LZF92_09720"
                     /product="HAD family phosphatase"
                     /protein_id="WP_081539226.1"
                     /sec_met_domain="HAD_2 (E-value: 6.9e-42, bitscore: 136.7,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKAAIFDLDGLLVDTEIVSLKIYQELLSEYGYSFTKEEYAKHYSG
                     KTEKENIKRFIDTYNLSVSYQECLDEVLNIERKLISQGVELKVGVKSLLMYLKENNYKV
                     ALATSSTKDRAISILKGYDILSYFDEFVFSEDVTLSKPNPEVFLKACEKTNVSTNEAIV
                     LEDSENGIMAAYNAHIPVICIPDMKRPAKEYLEKTIAVCKTLNDVIDYLKTK"
     gene            complement(2390..3361)
                     /locus_tag="LZF92_RS09725"
                     /old_locus_tag="LZF92_09725"
     CDS             complement(2390..3361)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703465.1"
                     /locus_tag="LZF92_RS09725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LZF92_09725"
                     /product="thioredoxin family protein"
                     /protein_id="WP_081539221.1"
                     /transl_table=11
                     /translation="MRYIKFTLLIYSFLLTWGITTNSISADEVTPTTTSETTVTPQSQS
                     TPVDSNDEDAAIEEKFYSHDVTIDKYNQNVANFNKVNTDTVKTMVTENSNNLDQVLYIG
                     RPTCYFCRQASPHLRDFNQLINNQLYYYDIDAEPNAHDFAFKEIGISGTPTTMRLKNGK
                     VVSAWIGGEKTGQEFYDFLFSADANKLADSYIIKKTVSTDTSNPVITNNQNVEPTVKPI
                     STNINQLKEEKTTPELVNTTSQSSKPVKKIHNQTTNKKNKNSVLPQLGVVPLYHHPLFK
                     LPIPRIIPKQLERTIMLITFLVLYLGFLIYLWLSWKDINKRK"
     misc_feature    3497..3517
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 27.54"
                     /tool="antismash"
     gene            3715..3900
                     /locus_tag="LZF92_RS09730"
                     /old_locus_tag="LZF92_09730"
     CDS             3715..3900
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699409.1"
                     /locus_tag="LZF92_RS09730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LZF92_09730"
                     /product="hypothetical protein"
                     /protein_id="WP_141566911.1"
                     /transl_table=11
                     /translation="MNGVKAPVKISAWTNFKTLFNEPSGWWATYASGYIGDYLDFTNRK
                     IIPERKFKKKYKIVNK"
     gene            complement(3939..4136)
                     /locus_tag="LZF92_RS09735"
                     /old_locus_tag="LZF92_09735"
     CDS             complement(3939..4136)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="LZF92_RS09735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="LZF92_09735"
                     /product="hypothetical protein"
                     /protein_id="WP_234057878.1"
                     /transl_table=11
                     /translation="MIWTLVDGNLIHLGILAFSSLVTTMLHFHYFESTDDKHPLNRIDF
                     GLQLLFIFISIIKFFLISGR"
     gene            4435..4608
                     /locus_tag="LZF92_RS09740"
                     /old_locus_tag="LZF92_09740"
     CDS             4435..4608
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703486.1"
                     /locus_tag="LZF92_RS09740"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LZF92_09740"
                     /product="bacteriocin"
                     /protein_id="WP_041823193.1"
                     /transl_table=11
                     /translation="MNNNFVQVDKKELAHIIGGRNSYDYIDSGQFGYDIGCTIANTKFF
                     KRLRHSNQNICS"
     gene            4738..4995
                     /locus_tag="LZF92_RS09745"
                     /old_locus_tag="LZF92_09745"
     CDS             4738..4995
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708569.1"
                     /locus_tag="LZF92_RS09745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LZF92_09745"
                     /product="bacteriocin"
                     /protein_id="WP_011476663.1"
                     /transl_table=11
                     /translation="MLKKLWETWLDGGFIRGKKRYVIAPVIWALLIPLGIWLFGNEEMS
                     YLDYIQTPKMIIVTIFCLVGGSTLLYLLDTAMKVNKHMKG"
     gene            5001..5195
                     /locus_tag="LZF92_RS09750"
                     /old_locus_tag="LZF92_09750"
     CDS             5001..5195
                     /GO_process="GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703435.1"
                     /locus_tag="LZF92_RS09750"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LZF92_09750"
                     /product="Blp family class II bacteriocin"
                     /protein_id="WP_003703435.1"
                     /sec_met_domain="Bacteriocin_IIc (E-value: 1.4e-16,
                     bitscore: 53.8, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MMKEFTVLTECELAKVDGGKRGPNCVGNFLGGLFAGAAAGVPLGP
                     AGIVGGANLGMVGGALTCL"
     gene            5213..5419
                     /locus_tag="LZF92_RS09755"
                     /old_locus_tag="LZF92_09755"
     CDS             5213..5419
                     /GO_process="GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703485.1"
                     /locus_tag="LZF92_RS09755"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LZF92_09755"
                     /product="Blp family class II bacteriocin"
                     /protein_id="WP_003703485.1"
                     /sec_met_domain="Bacteriocin_IIc (E-value: 4.4e-11,
                     bitscore: 36.3, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKNLDKRFTIMTEDNLASVNGGKNGYGGSGNRWVHCGAGIVGGAL
                     IGAIGGPWSAVAGGISGGFTSCR"
     gene            5851..5967
                     /locus_tag="LZF92_RS09760"
                     /old_locus_tag="LZF92_09760"
     CDS             5851..5967
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708563.1"
                     /locus_tag="LZF92_RS09760"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LZF92_09760"
                     /product="induction peptide AbpIP family protein"
                     /protein_id="WP_003703471.1"
                     /transl_table=11
                     /translation="MKFEVLTEKKLQKIAGGATKKGGFKRWQCIFTFFGVCK"
     misc_feature    6178..6196
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 25.5"
                     /tool="antismash"
     gene            6238..6738
                     /locus_tag="LZF92_RS09765"
                     /old_locus_tag="LZF92_09765"
     CDS             6238..6738
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="LZF92_RS09765"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="LZF92_09765"
                     /product="hypothetical protein"
                     /protein_id="WP_014573633.1"
                     /transl_table=11
                     /translation="MLDLFSYITFGASALFFTLFTNYYTMVSVIILDVSLNIFEYIILT
                     KTTIIEKLDDEYGLLYFYLILYIWISSMVIYYLTTYVFNIDAVSYIIATVVVVSQLFFS
                     LFVYSINKKIRQDKLSLQQINDLKIYTQQLESSQRGLRKFKHDYQNMLLSLKLSVKKVM
                     IKS"
     gene            6852..7250
                     /locus_tag="LZF92_RS09770"
                     /old_locus_tag="LZF92_09770"
     CDS             6852..7250
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF025854.4"
                     /locus_tag="LZF92_RS09770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LZF92_09770"
                     /product="GHKL domain-containing protein"
                     /protein_id="WP_014573632.1"
                     /transl_table=11
                     /translation="MNRIFQNNIQYSFECRIVIENLSNKYNSFDIVRILGIVYDNAIEE
                     SLEFGDEAKIDTMIYQENGELEIEIRNRYRDTDLTIQDIKKSGFTTKKNHDGLGLANIE
                     ELSQSYNDIFINYQIKDGWFTFLMVMSI"
     gene            7264..7818
                     /locus_tag="LZF92_RS09775"
                     /old_locus_tag="LZF92_09775"
     CDS             7264..7818
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /locus_tag="LZF92_RS09775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LZF92_09775"
                     /product="response regulator"
                     /protein_id="WP_234057879.1"
                     /transl_table=11
                     /translation="MEEYGIIICDDEKDQVRRIHDLVKYAIWSLEDSADSKGKKFKITL
                     EANSYIEVADYLEDNKVDSGVYFLDIELSKDKADRDGIDLAEFIRQKDPNAQIIFITAY
                     ENLAAMTYRRRTGALDFITKNDNDEVIQKRLNETLSLAARRIRESHAIKKMTFSYKWAE
                     ELLTKISMIFYILLPLLSHID"
     gene            7743..8057
                     /locus_tag="LZF92_RS09780"
                     /old_locus_tag="LZF92_09780"
     CDS             7743..8057
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF016296.4"
                     /locus_tag="LZF92_RS09780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LZF92_09780"
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="WP_234057880.1"
                     /transl_table=11
                     /translation="MGRRIINENINDILYIITTPKSHRLKIVTVNGMGEFTGDIKKLEV
                     ENDFLEKISQSCLANPSNIVEINLSTRSISFKDGYKETFPRRSKAKMKSLIERYNLKTV
                     "
     gene            8093..8332
                     /locus_tag="LZF92_RS09785"
                     /old_locus_tag="LZF92_09785"
     CDS             8093..8332
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699418.1"
                     /locus_tag="LZF92_RS09785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LZF92_09785"
                     /product="hypothetical protein"
                     /protein_id="WP_041823186.1"
                     /transl_table=11
                     /translation="MVETSIIILLIVGFMVISRLKVNFTRNSKYAGFLLLILLNLNGLM
                     RTPMLTMRGLILLGTLMIFIVMLIKEIRQNRKYN"
     gene            complement(8485..8628)
                     /locus_tag="LZF92_RS09790"
                     /old_locus_tag="LZF92_09790"
     CDS             complement(8485..8628)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="LZF92_RS09790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="LZF92_09790"
                     /product="hypothetical protein"
                     /protein_id="WP_160944120.1"
                     /transl_table=11
                     /translation="MNKRIDYFIGIANICTGILMLFNSNNLYPLIFCMMGLISIIATTL
                     KK"
     gene            8876..9073
                     /locus_tag="LZF92_RS09795"
                     /old_locus_tag="LZF92_09795"
     CDS             8876..9073
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="LZF92_RS09795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="LZF92_09795"
                     /product="hypothetical protein"
                     /protein_id="WP_081539219.1"
                     /transl_table=11
                     /translation="MKINKNFYKNYTFYIGLVMLSIAVGSIFLDKGYFLFAIIDILFTY
                     LGIRYVYQSLVTTKRYERKR"
     gene            complement(9209..9343)
                     /locus_tag="LZF92_RS10355"
     CDS             complement(9209..9343)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="LZF92_RS10355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_267966586.1"
                     /transl_table=11
                     /translation="MVKNIQKIFINYYFLERFTFAATKIRNSGIIPATHPYLKISIIQ"
     misc_feature    complement(9320..9334)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 23.77"
                     /tool="antismash"
     gene            9419..10261
                     /locus_tag="LZF92_RS09800"
                     /old_locus_tag="LZF92_09800"
     CDS             9419..10261
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="LZF92_RS09800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="LZF92_09800"
                     /product="hypothetical protein"
                     /protein_id="WP_014573629.1"
                     /transl_table=11
                     /translation="MEEKFLNYDIPNFVKESPNYLKLFEKERIILKNKHDKWGIYFSEE
                     LGYSLEYKENLGKVDYVYDSFLEAYSQADNDGEVWFSDEIAHEVFGEPEDEDLNEKGEF
                     GNLSDDFFWTKNHLIFRHHGDYSVKNLEDEYQEWKNLDKKVKEDSFTHENFLLIYEWLR
                     THPNFWKENVHGNRVFWETSHMNLNFMDIYPTSSETSGKVEWWAEGGYHVLDDTDVPYA
                     TTYYHDIALDCGGNTYEQAVLNFAKNVWLQTHGMLKDKEARRDAIFERWVKDSGCESD"
     misc_feature    10381..10394
                     /note="TFBS match to RpoN, Regulator of nitrogen
                     assimilation and virulence, confidence: medium, score:
                     15.94"
                     /tool="antismash"
ORIGIN
        1 aatccaacgg gtgcgaccgc aactcgagaa ttttacgaag aattagttgc ttgggcaaag
       61 aaatatgaag taggtatcat cagcgatttt gcatatggtg caattggttt tgatggagag
      121 cgaccattaa gttttatgga gattccagga gctaaggatg ttggtatcga attttatacc
      181 ttctctaaga cttttaacat ggcaggctgg cgagttgctt ttgcggtagg aaatgctgat
      241 atcattgatg ctttaaatct tatccaagat catttatttg taagtatctt tccagctcta
      301 caatatgcag gaattgatgc tttgaaagcc ccagaacgcg atgctgaaat cagtaagatt
      361 gtagatcgtt acgaaactag aagaaatgcg ttcatccaag ctgctgaaaa aatcggatgg
      421 aaagcttttg tacctaaggg aacgttctac gcttggatgg aagtaccaaa gggatatagt
      481 agtgaagaat ttgcagatct gcttttaaat gaagctggag tagcagtagc tcctgggaat
      541 ggttttggcg aatatggaga aggttatgta cgaattggtt tgttaattga accagaacgt
      601 ttagaagagg cagttgatag aatcgcaaaa cttaatttat ttaaatagtt gggaatagtg
      661 gcaagacagc gatgtttttg tcgctatttt tttgaaaaga taaattattg aaataatata
      721 catggatatg ttgttaacca aataagcaga aaatagtatt aattaagaaa atatagaatt
      781 aaatttaatt aaaatagtat aataatggca aaaatagaga ataggtggtg gaatgatgga
      841 ttatttagtt attaaggcaa ttatttggat attttttata acatcagcaa ttttggaagt
      901 taattataag aaaaaatatg gtgaattaga taaacgagct agaaagcaag caacattagg
      961 aacatttcta atagtaacta caatatttga tgaaggttat gagaataaaa tactatatat
     1021 aatagtatta atagcactgt tggtatatgt agtattagtg atatatagat tagttatggc
     1081 tattaaggat aggaaaaagt aataagaaag gtgataaagc aaatgaaagc aatctataga
     1141 ggtagaaaag tagatgtttg gcaagtgagt agagataatg tccaacctga ttgggttaaa
     1201 gatgcttttg agcataatgg gttgcgatgg ctacatggca gtggtaataa agtcagaatt
     1261 tattggccag cagttactag ggcaaaaaag ggaatagtac gaactatttt gaatgatatt
     1321 tcagcttctt ttgttggtat agctggtggc agcgtgtcga tgtatgacat tggctttgag
     1381 ggagatgtta tcgattttac caattctaag gtcgttacag ccgaaaagtt cgccaagaaa
     1441 tatcggatag tgtctgaaga ttgaggtaaa ggtattaaat tgtctatgac aagataatca
     1501 acttttacgt caaaataaga ttgaataatt atatgatata ataaatatgt attatgaaag
     1561 gtggttgatt tattgtgatt aaagcggcta tatttgatct tgatggattg ctagtggata
     1621 cagaaatcgt atctttaaaa atatatcagg aattattatc tgaatatgga tatagtttta
     1681 ctaaagaaga atatgcaaaa cattatagtg ggaaaacaga aaaagaaaat attaaacgtt
     1741 ttatagatac atataactta tcggtatctt atcaggaatg tttagatgaa gttttgaata
     1801 ttgaacgcaa acttatttca caaggtgtgg aattaaaagt aggggtaaaa tctctactta
     1861 tgtacctaaa agagaataat tataaagtag ctttggcaac atcaagtacc aaagatagag
     1921 cgatttctat tttaaaaggg tacgatattc tcagctactt tgacgaattt gtatttagtg
     1981 aagatgtaac actaagtaag cctaatccag aagttttctt aaaagcttgt gaaaaaacaa
     2041 atgtatcaac taatgaagca atagttttag aagatagtga aaatggtatt atggctgcct
     2101 acaatgcaca tattccagtt atttgtatac ctgatatgaa aagaccagca aaagaatatt
     2161 tagagaaaac tattgctgtt tgtaaaacat taaacgatgt tattgattat ttaaaaacaa
     2221 aataacagtt accgcataat aaaagtaaca taataaaaaa agttgaatat aaagatagga
     2281 ccaatttact aatttatgca aaaatgtagt tttgaatcta caaaaatcga ttatttgcat
     2341 aaattttcta attggtccta ttgctatatc gcatatttta cgtctttatc tacttccttt
     2401 tatttatgtc tttccagctc aaccataaat atattaagaa ccccaaatac agaactagaa
     2461 aagtaattaa cattatagta cgttctaatt gcttagggat aattcgagga ataggaagtt
     2521 taaataatgg atgatgataa agagggacaa cccctaattg tggtagaaca gaatttttgt
     2581 ttttcttatt agtagtttga ttatgaattt ttttaacagg tttagatgat tgtgaagtag
     2641 tattaaccaa ttcaggagta gttttttcct ctttaagctg atttatattt gttgatatag
     2701 gcttaacagt tggttcaaca ttttgattat ttgtaataac aggatttgaa gtatccgtgc
     2761 ttacagtttt ctttatgata taggaatctg ctaacttgtt agcatctgca gaaaataaaa
     2821 agtcataaaa ttcttgtcct gttttttcgc caccaatcca agcactgaca actttaccat
     2881 tctttaaacg catagttgtt ggggttccag aaattccaat ttctttaaat gcaaaatcat
     2941 gagcattagg ttcagcatca atatcatagt agtaaagctg attatttatt agttggttaa
     3001 aatcacgtag atgtggagaa gcttgccgac aaaaataaca agtaggacgt ccaatgtaaa
     3061 gaacttggtc tagattgttg ctattttcag taaccatggt tttaacagta tcagtattaa
     3121 ctttattgaa gtttgccaca ttttgattat atttatcgat tgtaacatca tgagagtaaa
     3181 atttttcttc aattgcagca tcctcatcat tagaatcaac aggtgtactt tgactttgag
     3241 gcgtaactgt tgtttcagat gttgtagtag gtgtaacttc atccgctgat atactattcg
     3301 tggtgattcc ccaagttagt aaaaaagaat atattagtaa agtaaattta atgtatctca
     3361 ttttttcaat ccccctaatc gtatatatgt aattattgca aataatgtct acatagtgtg
     3421 ataaactgct ggaatggtag caataactac ttatttggac tatttaccaa ataagtaaga
     3481 aatagtgcta gttaagaaat ttagcattgg atgtattatg aaaatagtat aatgaagatg
     3541 taattaatag agctctttaa ttatatgtag aaggtgattt ctatgaaagc aatctataaa
     3601 ggtaaagaag tacaaatatg gcagataagt aaaacagcag aacaacctga atggataaaa
     3661 gaagcattta aagctaacta tctaaagtga tcaggtgata aattgttatt tattatgaat
     3721 ggagttaagg cacctgttaa aattagtgct tggacgaatt ttaagacttt atttaatgag
     3781 ccttcaggtt ggtgggcaac ttatgcaagt gggtatatag gcgattactt ggatttcact
     3841 aatcgtaaaa ttataccaga aagaaaattt aaaaagaagt acaaaattgt taataaataa
     3901 gcctatcagg tgtaatttga tacctgatag gcttattgtt atcttcctga aatcaagaaa
     3961 aacttaataa tagaaataaa gataaatagt aattgtaatc caaaatcaat tctatttaat
     4021 ggatgtttgt catcagtaga ttcaaaatag tgaaaatgta gcatggttgt aaccaaagaa
     4081 gaaaaagcca atataccaag atgaattaga tttccatcaa ctaatgtcca gatcaacagg
     4141 aataaagtaa ttagtgaaac agaaattttg ctgaaattaa tattattaga aaaactattc
     4201 ataatttttt acctcaattc attataaaag tatttttatt atcacataga aagtgatgaa
     4261 ttgaggggat ttttccaatt tggtagtaag cggtgcctat ctggttgaaa aaagagaata
     4321 agaataaaat ttagcaaaat tagaagaaat agtcaaaaag agcagatgaa gtgaataatt
     4381 ataagtgtaa attatagcta taataaattt ttaaaattat gaggtgttga agttatgaat
     4441 aataattttg tacaagttga taagaaagaa ttggcacata taattggtgg aagaaattct
     4501 tatgattata tagatagcgg acagtttggt tacgatatag gctgtacaat tgctaatact
     4561 aaatttttta aaagattaag acactcaaat caaaatattt gtagttaaaa agaaatgtaa
     4621 ccaactaggt atagaataac gtattttacg ctaaatcgat atttgaatac ggaatgataa
     4681 tacaataaaa gtgtaagtat atgtttcaca agtgttttag aaagggtgtg ttcttacatg
     4741 ctaaaaaaac tatgggagac ttggttggat ggtggattca ttagaggaaa gaagagatat
     4801 gttattgctc cagttatttg ggcactattg attccactag gaatatggct gtttggtaat
     4861 gaggaaatgt cttatttaga ttatattcaa acgcctaaaa tgataatagt tactattttt
     4921 tgtctagtag gtggtagtac actactttac ttattagata ctgcgatgaa agtaaataaa
     4981 catatgaaag gatgattatc atgatgaagg aatttacagt attgacagaa tgtgagttag
     5041 caaaggttga tggtgggaaa cgtggtccta actgtgtagg taacttttta ggtggtctat
     5101 ttgctggagc agctgcagga gttccacttg gaccagctgg tattgtaggt ggagcaaact
     5161 taggaatggt aggcggagca cttacttgtt tataatatag gagtaattta aaatgaaaaa
     5221 tctagataaa agattcacaa ttatgactga agataactta gcatcagtaa atggtggtaa
     5281 aaatggttat ggtggtagtg gaaatcgctg ggttcactgt ggagctggca tcgtaggtgg
     5341 agctttaatt ggagctatcg gtggaccctg gtcagccgta gcgggtggaa tttctggtgg
     5401 ctttacaagt tgccgttaga ataagtctaa tgaattagaa aataagaaat aaatctattt
     5461 ttcttcaagt agtgtcaagt gtaagttata gtatatagct aaatttgaca ctacttttat
     5521 ttttaataga acataatata ttttgggatt aggaagtgag tatatgatat ttgggattac
     5581 tgtaatagtt gcatatagta tttttatcag tatttatact agtctaaaag aagaggggca
     5641 tcataagtta tttgatgaga aagtcataga aaagaataag aaaataagtt atgtgagagc
     5701 gtgggtgtta aattttatgt ggtggctata gttttaagaa tttcatccaa ataagcatta
     5761 aaatgaaccg aatatgataa aaaacgaata aagtagatta aaaaatgtta aactatattt
     5821 gtaataaaga taacaaagga gagatttttt atgaagtttg aagtgttaac agaaaagaag
     5881 ttacagaaaa tagctggggg agcaaccaaa aagggtggct ttaaaagatg gcaatgtata
     5941 ttcacatttt ttggtgtttg taaataatgg ttaatttaaa ttcaacatct ggttatatag
     6001 tagctacact atatttataa atactttttg tagatatagt ttttttatct tttgacccct
     6061 actaggaaaa gtaagaagta taatttatta tttatacttc ttacttttcc tgtaattctt
     6121 ttaattaatg gtgttggaga tattattggt ttttttgcac catttattga aatttttatt
     6181 ttttatattg tatacagaca actaactttg gattacagat tatgtggaag gttgttaatg
     6241 ctagatttat tttcctatat aacattcggt gcttcagcat tattttttac attatttaca
     6301 aattattaca caatggtcag tgtgataata ttagatgtgt cattaaacat ttttgaatat
     6361 ataattttaa caaagacgac tataatagag aaactagatg atgagtatgg attattgtat
     6421 ttttatttaa ttttatacat atggattagt tcaatggtta tttactatct aacaacatat
     6481 gtatttaata tagatgcagt atcgtatatt atagctacgg tagttgttgt tagccaattg
     6541 ttcttttcat tatttgtata ttccattaat aagaaaatac ggcaggataa attaagttta
     6601 caacaaatta atgatctaaa aatttataca caacagttag aaagtagcca gcgaggttta
     6661 cgtaaattta agcacgatta tcaaaatatg ctattaagct tgaaactcag tgtaaaaaaa
     6721 gtcatgataa agagttaata gataaactag ctgaatattc aagtaagact ttagaagata
     6781 aagtattatg gcagtttaat gatgttgaca atgttaaaga tgaattatta aagagtctat
     6841 ttatcagtaa gttgaataga atctttcaaa acaatattca atattctttt gaatgtcgaa
     6901 ttgtgataga aaacttgtcg aataagtata actcgtttga cattgttaga atattaggaa
     6961 ttgtgtacga taatgcaatt gaagaaagtc ttgagtttgg tgatgaagct aagatagata
     7021 ctatgattta tcaagaaaat ggtgaacttg aaattgagat tagaaatcgt tatcgagata
     7081 ccgatctaac aattcaagat ataaaaaaat caggttttac aactaagaag aatcatgacg
     7141 gattaggatt agctaatata gaagaattaa gtcaatctta taatgatatt tttattaatt
     7201 atcaaattaa agatggttgg tttactttcc ttatggttat gagcatttaa ggggtagttt
     7261 aatttggaag aatatggaat tattatttgt gacgatgaga aggatcaagt tagaaggata
     7321 catgatctag ttaaatatgc tatttggtct ttagaagatt ctgctgacag taaaggtaaa
     7381 aaatttaaaa taactttgga agctaatagt tatattgaag tagccgatta tttggaagat
     7441 aacaaagtag atagtggagt ttatttttta gatattgaac ttagtaaaga taaagcagat
     7501 agagatggga tagatttagc tgaatttatt agacaaaaag atccaaatgc acagataatt
     7561 tttattacag cttatgaaaa tctagcagca atgacttatc gacgtcgtac tggagcattg
     7621 gattttatta ctaaaaatga taatgatgaa gtgattcaaa aacgactaaa tgagacttta
     7681 tcattagctg caaggagaat tagggaatct catgccataa agaaaatgac atttagctat
     7741 aaatgggcag aagaattatt aacgaaaata tcaatgatat tctatatatt attaccactc
     7801 ctaagtcaca tagattgaaa atagttacag ttaatggtat gggtgagttt acaggcgata
     7861 ttaagaagtt agaggtagaa aatgattttc tagaaaaaat atcacaatct tgtttagcta
     7921 atccaagtaa tattgttgaa attaacctgt caacaaggag tatttctttt aaagatggat
     7981 ataaggagac ttttcctagg agaagtaagg ctaaaatgaa aagtttgatt gaaaggtata
     8041 atttgaaaac agtttaattt tttgtgttta ttagtgtagg tggtgaatga tcatggtaga
     8101 aacaagtatt attatattgc taattgtagg gtttatggta atttcaaggt tgaaagtgaa
     8161 ttttacgaga aatagtaagt atgcagggtt tctattgtta atattactga atttaaatgg
     8221 gcttatgcga actcctatgt taacaatgag aggtttaatc ttattgggaa cattaatgat
     8281 ttttatagtt atgttgatta aagaaattag acaaaataga aaatataatt aataaagagg
     8341 gttggaactt agggtattcc aaccctcttt atatgacatc tcatgattat ctttgcaaat
     8401 gtttattgac tattattaat atatttacaa tataaagtaa tgggtcgcta aatatgttaa
     8461 acaaaattag ataaaagtta gtattcactt cttcaatgta gttgcaatta tagaaattaa
     8521 tcccatcata caaaaaatta agggatataa gttattacta ttaaacaaca ttaggattcc
     8581 tgtacatata ttggctatac caataaagta gtctattctt ttattcactt ttttcacatc
     8641 cttatttaag ataattacct atagttattg tatttcaaat gaaagatagg atgatttttc
     8701 aagcctaata tgaacttttt aaagcgtatt tggttaagcc agatatgtat taaaaaagac
     8761 ctaattcgat aatttaggca cgatgatttg agaatatgat aaattaaaac taggaaatga
     8821 aattatataa tggaaaaatc tagctgaagt tattagtaga aatggagagt aatatatgaa
     8881 aataaataaa aatttttata aaaattatac tttttatatc ggtttagtaa tgttatcaat
     8941 agcagtagga agtatttttc ttgataaagg atatttttta tttgcaatta ttgatatatt
     9001 atttacttac ctaggtataa gatatgtcta tcaatcattg gtgacaacaa aacgttatga
     9061 aagaaagcgt taaaatcaat agagtaagaa agttaggaaa aatcaaatta aaaaagtata
     9121 tttttactta atgatttttc ttaatttttt attttgaagt taaggagtac atatgaaaaa
     9181 gtcaatacta gtttttattg tattattact attgaataat tgatattttt aaatatgggt
     9241 gagttgccgg tattattcca ctatttctta tcttagttgc tgcaaaagta aaacgttcta
     9301 aaaaataata attaataaaa attttttgaa tattctttac cattaaacca ctggtagatt
     9361 tttatttcgt aaagattagg gtaggattat tgtagattaa caagtgagga tgaatgacat
     9421 ggaagaaaaa tttttgaatt atgatatacc taattttgta aaagaatcac ctaattacct
     9481 aaaactattt gaaaaagaac gtatcatcct aaagaataaa catgataaat gggggatcta
     9541 ctttagtgag gaattaggat attcattgga atataaggaa aatttaggaa aagtagatta
     9601 tgtatatgat tctttcttgg aagcctactc tcaagctgat aatgacggag aagtttggtt
     9661 ttcagatgaa atagcacatg aagtatttgg tgaacctgaa gatgaagatc ttaatgaaaa
     9721 aggagaattt ggaaatttga gcgatgattt cttttggaca aagaatcacc taattttcag
     9781 acaccatggt gactatagtg ttaagaacct agaagatgag taccaagaat ggaaaaattt
     9841 ggataagaaa gtcaaagaag acagttttac gcatgaaaat tttctattaa tttatgaatg
     9901 gcttaggaca catcctaatt tctggaaaga aaatgttcat gggaatagag tcttctggga
     9961 gacatcacac atgaatttaa actttatgga tatttatcca acttctagtg aaacaagtgg
    10021 aaaagtagaa tggtgggctg agggtggata tcatgtttta gatgatactg atgtcccata
    10081 cgcaacaact tactatcatg atattgcttt ggattgtggt ggtaatacat atgagcaagc
    10141 agtactgaat tttgcgaaaa acgtttggtt acaaacacat ggaatgttga aggataaaga
    10201 agcaagacgt gacgctatct ttgaaagatg ggttaaggat tctggatgcg aatctgactg
    10261 aatacttaag ataaaattta atagcttacg ccaagtaaaa atcgccaagg attagtgcaa
    10321 atctttggcg attttttaaa tttttccggt ttctacttga aagttaatca tggtgcaaaa
    10381 ggcatttcta atgctaaaaa aataccaacg aaaattaaa
//
