LOCUS       NZ_CP085300            10399 bp    DNA     linear   CON 18-JAN-2024
DEFINITION  Streptomyces sp. BSE6.1 chromosome, complete genome.
ACCESSION   NZ_CP085300
VERSION     NZ_CP085300.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. BSE6.1
  ORGANISM  Streptomyces sp. BSE6.1
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8024389)
  AUTHORS   Ramesh,C., Anwesh,M., Vinithkumar,N.V., Kirubagaran,R. and
            Dufosse,L.
  TITLE     Complete Genome Analysis of Undecylprodigiosin Pigment
            Biosynthesizing Marine Streptomyces Species Displaying Potential
            Bioactive Applications
  JOURNAL   Microorganisms 9 (11), 2249 (2021)
   PUBMED   34835376
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8024389)
  AUTHORS   Chatragadda,R. and Maile,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-OCT-2021) Andaman and Nicobar Centre for Ocean Science
            and Technology, National Institute of Ocean Technology, Dollygunj,
            Port Blair, Andaman and Nicobar Islands 744103, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 10-OCT-2021
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/18/2024 07:10:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,273
            CDSs (total)                      :: 7,198
            Genes (coding)                    :: 7,094
            CDSs (with protein)               :: 7,094
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 47 of 104
            Pseudo Genes (incomplete)         :: 80 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 30 of 104
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 01:03:07
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6343948
            Orig. end                         :: 6354347
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP085300.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from National Institute of Ocean
            Technology.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10399
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="22"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10399
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10399
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5399](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5399)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    583..598
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: medium, score: 21.08"
                     /tool="antismash"
     gene            1358..1966
                     /locus_tag="LJA35_RS28420"
     CDS             1358..1966
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016436202.1"
                     /locus_tag="LJA35_RS28420"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF1349 domain-containing protein"
                     /protein_id="WP_233647051.1"
                     /transl_table=11
                     /translation="MTLQLPELPFPLRPYGPDGRWAYADGVLTGVAGSRQDRFVPPTGE
                     SLESVSDAPRLLGAPEGDFQLIARVSVGFRGAFDAGVLYVHVGERAWAKLCLEYSPDVA
                     TVCTVVTRGHSDDCNSFTVDGESVWFRVSRSDRAFAFHASLDGERWTFVRLFTLGGERE
                     TGAALVGFLAQAPTGEGCTVTYDHLEFRPGWPADLRDGS"
     misc_feature    complement(1375..1388)
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: weak, score: 17.76"
                     /tool="antismash"
     gene            2060..2773
                     /locus_tag="LJA35_RS28425"
     CDS             2060..2773
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003976951.1"
                     /locus_tag="LJA35_RS28425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DsbA family oxidoreductase"
                     /protein_id="WP_233647052.1"
                     /transl_table=11
                     /translation="MRVEIWSDIACPWCYVGKARFEKALAAFPHRDGVEVVHRSFELDP
                     GRAKDDVQPVLTMLTAKYGMSEAQAQAGEDNLGAQAAAEGLDYRTRDRDHGSTFDMHRL
                     LHLAKERGRHEALLDAFYRGNFADERSVFNDDERLVDLAVGAGLDADEVRSVLADPDAY
                     ADDVRADEREAAQLGATGVPFFVLDRAYGVSGAQPAEVFTRALTQAWGERTPLKLVDDG
                     GAEACGPDGCAVPQN"
     gene            2845..3939
                     /locus_tag="LJA35_RS28430"
     CDS             2845..3939
                     /NRPS_PKS="Domain: Aminotran_5 (28-305). E-value: 8.4e-23.
                     Score: 72.8. Matches aSDomain:
                     nrpspksdomains_LJA35_RS28430_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448151.1"
                     /locus_tag="LJA35_RS28430"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_233647053.1"
                     /sec_met_domain="Aminotran_5 (E-value: 1.6e-17, bitscore:
                     62.2, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDGEVTGPHSGAMESFENLVRTEFAPKTTYLNTASTGLLPARTLV
                     AMRDAAASAAAGQPLDMFADVEAARAAYARLVGVPATRVAIGASVATFSGLVAASLPPG
                     AEVLTAEADFSSLVNPLHARGDLKVRSVPLERIAESVGPDTALVAVSSVQSADGRIADL
                     AGLRDAARAHGARTLIDVSQANGWLPTAADDFDFVTAVGYKWVLCPRGVAFLVVPEDLG
                     AAAGLTPLFAGWATGERPWDSCYGPVEEYAHSARRFDESPALFSYTGARHSLALIEELG
                     VDAVHAHDTGLADRFRAGVRSLGHEPVDAPGSAIVSVPGLGHRQGELGGAGVQVSDRAG
                     NLRAAFHVYNTEADADRLLDVLSG"
     aSDomain        2929..3759
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_LJA35_RS28430_Aminotran_5.1"
                     /evalue="8.40E-23"
                     /label="LJA35_RS28430_Aminotran_5.1"
                     /locus_tag="LJA35_RS28430"
                     /protein_end="305"
                     /protein_start="28"
                     /score="72.8"
                     /tool="antismash"
                     /translation="TYLNTASTGLLPARTLVAMRDAAASAAAGQPLDMFADVEAARAAY
                     ARLVGVPATRVAIGASVATFSGLVAASLPPGAEVLTAEADFSSLVNPLHARGDLKVRSV
                     PLERIAESVGPDTALVAVSSVQSADGRIADLAGLRDAARAHGARTLIDVSQANGWLPTA
                     ADDFDFVTAVGYKWVLCPRGVAFLVVPEDLGAAAGLTPLFAGWATGERPWDSCYGPVEE
                     YAHSARRFDESPALFSYTGARHSLALIEELGVDAVHAHDTGLADRFRAGVRSLGH"
     gene            complement(4098..4994)
                     /gene="thpD"
                     /locus_tag="LJA35_RS28435"
     CDS             complement(4098..4994)
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448161.1"
                     /locus_tag="LJA35_RS28435"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ectoine hydroxylase"
                     /protein_id="WP_165285737.1"
                     /sec_met_domain="PhyH (E-value: 9.3e-42, bitscore: 142.4,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTTTNVTDLYPTRGATEVATPRQDPVVWGSPDTPGPVSAGDLQS
                     LDRDGFLAVDQLIAPDEVAEYRRELERLTTDPAIRADERSIVEPQSKEIRSVFEVHKIS
                     EVFAKLVRDERVVGRARQILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLPN
                     MRTISVSIALTENYDTNGGLMIMPGSHKTFLGCAGATPKDNYKKSLQMQDAGTPSDEAL
                     TKMASEYGIKLFTGKAGSATWFDCNCMHGSGDNITPFPRSNVFIVFNSVENTAVEPFAA
                     PIRRPEFIGARDFTPVR"
     misc_feature    complement(4985..5005)
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 19.15"
                     /tool="antismash"
     gene            complement(5001..5399)
                     /locus_tag="LJA35_RS28440"
     CDS             complement(5001..5399)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010037840.1"
                     /locus_tag="LJA35_RS28440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ectoine synthase"
                     /protein_id="WP_030184764.1"
                     /sec_met_domain="ectoine_synt (E-value: 5.7e-54, bitscore:
                     180.0, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFKEFEGTDRHVKSASGTWESTRIVLAKEKVGFSLHETILY
                     AGTETSMWYANHIEAVVCTKGDAELTDRETGKTYHITPGTMYLLNGHERHTLKVKEDFH
                     CICVFNPPVTGREDHDENGVYPLLTEEV"
     gene            complement(5526..6797)
                     /gene="ectB"
                     /locus_tag="LJA35_RS28445"
     CDS             complement(5526..6797)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (29-366). E-value: 1.6e-72.
                     Score: 236.3. Matches aSDomain:
                     nrpspksdomains_LJA35_RS28445_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448159.1"
                     /locus_tag="LJA35_RS28445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_102929418.1"
                     /sec_met_domain="Aminotran_3 (E-value: 4.5e-76, bitscore:
                     254.8, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITQPDLSVFETVESEVRSYCRGWPTVFDRAVGSRMYDEDGHEY
                     LDFFAGAGSLNYGHNNAVLKRALIDYLERDGVTHGLDMSTTAKRRFLETFQNTILRPRD
                     LPYKVMFPGPTGTNAVEAALKLARKVKGRESIVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDNYFDGTVEDFIWFERLLEDQGSGLNKPAAVIVETVQGEGGINVARA
                     EWLRALAALCERQDMLLIVDDIQMGCGRTGAFFSFEEAGITPDIVTVSKSISGYGMPMA
                     LTLFKPELDVWEPGEHNGTFRGNNPAFVTATATLEAYWADGSAMEKQTRKRGEQVEQHM
                     IAITEENLADVKEYRGRGLVWGLEFHDKGRAGRVAKRAFELGLLIETSGPESEVVKLLP
                     ALTITPDELDEGMKTLARAVRETA"
     aSDomain        complement(5700..6710)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_LJA35_RS28445_Aminotran_3.1"
                     /evalue="1.60E-72"
                     /label="LJA35_RS28445_Aminotran_3.1"
                     /locus_tag="LJA35_RS28445"
                     /protein_end="366"
                     /protein_start="29"
                     /score="236.3"
                     /tool="antismash"
                     /translation="DRAVGSRMYDEDGHEYLDFFAGAGSLNYGHNNAVLKRALIDYLER
                     DGVTHGLDMSTTAKRRFLETFQNTILRPRDLPYKVMFPGPTGTNAVEAALKLARKVKGR
                     ESIVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDNYFDGTVEDFIWFERL
                     LEDQGSGLNKPAAVIVETVQGEGGINVARAEWLRALAALCERQDMLLIVDDIQMGCGRT
                     GAFFSFEEAGITPDIVTVSKSISGYGMPMALTLFKPELDVWEPGEHNGTFRGNNPAFVT
                     ATATLEAYWADGSAMEKQTRKRGEQVEQHMIAITEENLADVKEYRGRGLVWGLEFH"
     gene            complement(6923..7435)
                     /gene="ectA"
                     /locus_tag="LJA35_RS28450"
     CDS             complement(6923..7435)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448158.1"
                     /locus_tag="LJA35_RS28450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_125632783.1"
                     /transl_table=11
                     /translation="MTAAQADLQIDRPRVADGAALWRIARDSKVLDLNSSYSYLLWCRD
                     FAATSAVARDENGEPVGFVTGYVRPDRPDTLLVWQVAVDEAYRGRRLAASLLDGLAERT
                     VAEQGLTTLETTISPDNTASQRLFTSFAERRGARVEREVLFDTAVFPDGPHEPEVLYRI
                     GPLSATG"
     misc_feature    complement(7434..7446)
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 17.04"
                     /tool="antismash"
     misc_feature    7445..7460
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 16.79"
                     /tool="antismash"
     misc_feature    complement(7591..7606)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     misc_feature    7601..7616
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.62"
                     /tool="antismash"
     misc_feature    7675..7691
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 19.05"
                     /tool="antismash"
     gene            complement(7765..8916)
                     /locus_tag="LJA35_RS28455"
     CDS             complement(7765..8916)
                     /NRPS_PKS="Domain: Aminotran_5 (72-299). E-value: 3.6e-29.
                     Score: 93.7. Matches aSDomain:
                     nrpspksdomains_LJA35_RS28455_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003976957.1"
                     /locus_tag="LJA35_RS28455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_212729842.1"
                     /sec_met_domain="Aminotran_5 (E-value: 7.2e-22, bitscore:
                     76.5, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTHPFLDLAPLSAGRFAAIEDRVARLLGTAQDVVVMQGEALLPLE
                     GAIRAAAGPGTVALNVITGPYGQTFGNWLRDCGATVHDLAVPFHTAVSAEQIGRALAEH
                     PEIDFVSLVHAEAATGNTNPVAEIGEVVRAHGALFYLDAVASVGAEPVLPDAWGVDLCV
                     IGAQKAMGGPAGVSAVSVSERAWARMAANPNAPRRSYLSLLDWKERWIDGGRKALLHAP
                     AQLEMLALEACVERIEAEGAATVMARHASAAAATRAGALALGGLEPYVYEAKDAAPVAT
                     TLRTPADVDASALVARALESDPALPLAAGGGALAGEMIRVNHYGPDATRGAVQGCLAAL
                     GAALAERGRKADLEAARRAVEDAWEAPAGTGASADSSNDATRG"
     aSDomain        complement(8020..8700)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_LJA35_RS28455_Aminotran_5.1"
                     /evalue="3.60E-29"
                     /label="LJA35_RS28455_Aminotran_5.1"
                     /locus_tag="LJA35_RS28455"
                     /protein_end="299"
                     /protein_start="72"
                     /score="93.7"
                     /tool="antismash"
                     /translation="WLRDCGATVHDLAVPFHTAVSAEQIGRALAEHPEIDFVSLVHAEA
                     ATGNTNPVAEIGEVVRAHGALFYLDAVASVGAEPVLPDAWGVDLCVIGAQKAMGGPAGV
                     SAVSVSERAWARMAANPNAPRRSYLSLLDWKERWIDGGRKALLHAPAQLEMLALEACVE
                     RIEAEGAATVMARHASAAAATRAGALALGGLEPYVYEAKDAAPVATTLRTPADVDASAL
                     VARAL"
     gene            9094..9594
                     /locus_tag="LJA35_RS28460"
     CDS             9094..9594
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011028017.1"
                     /locus_tag="LJA35_RS28460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_233647054.1"
                     /transl_table=11
                     /translation="MLALVPLVLVAVVTAVVLRRGAGAYPRARLPPAALAPAPRKPGAP
                     FRVVAAVAGWAAGLLYVWGLVCVGFAAMEAEDGGTDSAPPRPCRTGVSPELSGRVVDHS
                     VSYLPLRFRCETADGGAYDSADVPGYVNPGVAVLALTAVAGAVAAGWATESRARAAARG
                     GGR"
ORIGIN
        1 agggcgagta cctcggtgtc gtcgtcaacc accccgacga caccgccacc ccgcccatcc
       61 ccgacctgcg ccgctacaag gtgccgctcg gcgtcgtcgc cgtctactcc gcctccaact
      121 tccccttcgc cttctccgtc cccggcggcg acaccgccag cgccctcgcg gccggctgcc
      181 cggtcgtcgt caaggcgcac cccgaccacc cggccctgtc cgagctggtc gccaaggtgc
      241 tgcgccgggc cgcggcccgc catgacatcc cggacggcgt cctcggcctg gtccacggct
      301 tcgaggcggg cgtcgagctg atcaagcacc cgctggtcgc cgcggccggc ttcacgggct
      361 ccgtccgcgg tggccgcgcg ctgttcgacg cggcggccgc ccgtcccgtg ccgatcccct
      421 tccacggcga gctgggctcc ctgaacccgg tcgtcgtcac ggaggcggcg gccgtggagc
      481 gggcggaggc gatcggcgcg gggctggccg gttccatgac gctgggcgtc gggcagttct
      541 gcgtcaagcc cggcctggtc ctcgtgccgg ccggtgacgc cggtgacggc ctggtcaagt
      601 ccctcaccga cgcggtcagc gccaccgacg ccggggtgct cctcgaccac cgcatgcggg
      661 acaacttcgt cgcgggcgtc gccgagcgcg ccgcgctgcc cgacgtggac tccccggtga
      721 cgccgggatc cggcggcgag cacaccgtca gcgccgggtt cctcacggtg ccggcggcgc
      781 gactggcggc ggagggcgag cacgacctgc tcctggagga gtgcttcggg ccggtcaccg
      841 tggtggcccg ctaccaggac gagggcgagg tgcgaggggt gctgtcccgg ctgccgggga
      901 acctcacggc caccgtgcag ctctccgccg aggaggtggc gggggagggc gccggagcgg
      961 ctctcctcgc ggaggtgacg ccgttggcgg ggcgggtgct cgtcaacggc tggccgacgg
     1021 gggttgccgt ggcggcggct cagcagcacg gggggcctta cccggcgacc acgtcgacgt
     1081 ccacgtcggt ggggggtacc gctgtcgagc ggtggctgcg gccggtggcc tatcaggggg
     1141 tgcccgaggc gttgctgccg gtggagttgc gggacgacaa tccgctgggg ctgccgcggc
     1201 ggttcaacgg ggtgctggag cggtagcgcg ccgcaggggt gcgggtgttt cgccgggtgc
     1261 ggcgccgtcg tggctggtcg cgcccacgcg gcggagccgc aaatcgatac agccccgcgc
     1321 ccctgagggc atgcactggg cctgcgatga tcagtgcatg actcttcagc ttcccgaact
     1381 gccgtttccc ctccgcccgt acgggcccga cggccgttgg gcctacgccg acggtgtgct
     1441 caccggggtc gccgggtccc ggcaggaccg gttcgtgccg ccgaccgggg agtcgttgga
     1501 gtccgtgtcc gacgcgccgc ggttgctggg ggcgcccgag ggggacttcc agctcatcgc
     1561 gcgggtgtcg gtcgggttcc ggggtgcctt cgacgccggg gtgctctacg tgcacgtggg
     1621 ggagcgggcc tgggccaagc tgtgcctgga gtactccccg gacgtggcca ccgtctgcac
     1681 ggtggtcacc cgggggcact ccgacgactg caactccttc accgtcgacg gggagtccgt
     1741 ctggttccgg gtcagccgca gcgatcgggc cttcgccttc cacgcctccc tcgacggcga
     1801 gcgctggacc ttcgtcaggc tcttcaccct ggggggcgag agggagacgg gcgcggccct
     1861 ggtcgggttc ctggcgcagg cgccgacggg ggagggatgc acggtgacgt acgaccacct
     1921 ggagttccgg ccgggctggc ccgcggatct gcgggacggc agctgaacgc cggggtggga
     1981 atgggcgcgg ctgcttgaac gttcacgtac ggggcggaga gggcctccgc cgccgtacga
     2041 cctggagaag agccgagaca tgcgcgtcga gatctggagc gacatcgcct gcccgtggtg
     2101 ctacgtgggc aaggcccgct tcgagaaggc gctcgcggcc ttcccgcacc gggacggggt
     2161 cgaggtggtc caccgctcct tcgagctgga ccccgggcgg gccaaggacg acgtccagcc
     2221 ggtcttgacg atgctcaccg ccaagtacgg catgagcgag gcccaggcgc aggccggtga
     2281 ggacaacctc ggcgcgcagg cggccgccga gggcctggac taccgcaccc gggaccgcga
     2341 ccacggcagc accttcgaca tgcaccgcct gctgcacctc gccaaggagc ggggtcggca
     2401 cgaggcgctg ctcgacgcct tctaccgggg caacttcgcc gacgagcgct ccgtgttcaa
     2461 cgacgacgag cggctcgtgg acctggccgt cggcgccggg ctcgacgcgg acgaggtccg
     2521 ttcggtgctc gccgaccccg acgcctacgc cgacgacgtg cgcgccgacg agcgggaggc
     2581 cgcccagctc ggcgccaccg gggtgccgtt cttcgtgctc gaccgcgcct acggcgtctc
     2641 gggcgcccag cccgccgagg tcttcacccg ggcgctgacc caggcgtggg gcgagcgcac
     2701 gccgctgaag ctggtcgacg acgggggcgc cgaggcctgc ggtccggacg ggtgtgcggt
     2761 gccccagaac tgagggtccg gggaaagcac caggtgagac gcggtgcata agcgttgctt
     2821 ggaggaatcg cgtagaaata cgcaatggac ggggaggtca cgggaccgca cagtggtgcc
     2881 atggagagct tcgagaatct cgtccgtacc gagttcgccc cgaagaccac gtacctgaac
     2941 accgccagca ccggtctgct gccggcccgc acccttgtcg cgatgcgtga cgcggcggcc
     3001 tccgcggcgg cggggcagcc gctcgacatg ttcgccgacg tcgaggcggc ccgggccgcc
     3061 tacgcccgcc tcgtcggggt tcccgccacc cgggtcgcga tcggcgcgtc ggtcgccacg
     3121 ttcagcgggc tcgtcgccgc gtcgctgccg ccgggtgcgg aagtcctcac cgcggaggcc
     3181 gacttcagct ccctggtgaa tccgctgcac gcgcgcggcg acctcaaggt gcgcagcgtg
     3241 ccgctggagc ggatcgccga gtccgtgggc ccggacaccg cgctcgtggc ggtcagctcc
     3301 gtgcagtccg ccgacgggcg gatcgccgac ctcgccgggc tgcgggacgc cgcccgggca
     3361 catggggcgc ggacgctgat cgacgtctcc caggcgaacg gctggctgcc gacggccgcg
     3421 gacgacttcg acttcgtgac cgccgtcggc tacaagtggg tgctctgccc gcgcggcgtc
     3481 gccttcctcg tcgtaccgga ggacctgggc gcggcggccg ggctgacccc actgttcgcg
     3541 gggtgggcga ccggggagcg cccctgggac agctgctacg gccccgtcga ggaatacgcg
     3601 cactccgccc ggcggttcga cgagtctccc gccctgttct cgtacaccgg cgcccggcat
     3661 tcgctggccc tgatcgagga actgggcgtc gacgccgtgc acgcccacga caccggcctc
     3721 gccgaccggt tccgcgcggg ggtgcgctcg ctgggccacg agccggtgga cgcaccgggc
     3781 tcggcgatcg tctcggtgcc gggtctcggt caccggcagg gcgagctggg cggggccggc
     3841 gtccaggtgt ccgaccgggc gggcaacctg cgggccgcct tccacgtgta caacaccgag
     3901 gccgacgcgg accggctgct cgacgtgctg tcgggctgag cccgagtccc ggggacagca
     3961 tcgggcaggg gcctcccgtc ccacacgaaa aggcccctgc ccgagtctca gacgcgggtc
     4021 acttcaccgg agtgaagtcc cgcgcgccaa tgctgtgatt tccgggggac aacccccgga
     4081 cccccggccg gctcggctca ccgtacggga gtgaagtccc gtgctccgat gaactccggc
     4141 cgccggatcg gcgccgcgaa cggctccacg gccgtgttct ccacgctgtt gaacacgatg
     4201 aacacgttgc tgcgcgggaa cggcgtgatg ttgtcgcccg acccgtgcat gcagttgcag
     4261 tcgaaccagg tcgccgagcc ggccttgccg gtgaacagct tgatgccgta ctcggaggcc
     4321 atcttcgtca gcgcctcgtc ggacggggtc cccgcgtcct gcatctgaag ggacttcttg
     4381 tagttgtcct tcggcgtggc ccccgcgcac ccgaggaacg tcttgtgcga ccccggcatg
     4441 atcatcagac cgccgttggt gtcgtagttc tccgtgagcg cgatcgagac cgagatcgtg
     4501 cgcatgttgg gcaggccgtc ctcggcgtgc caggtctcga agtccgagtg ccagtagaag
     4561 ccgctggccc cgaagccggg cttgacgttg atccgcgact ggtggacgta gacgtccgag
     4621 cccaggatct gccgggcccg cccgaccacg cgctcgtcgc gcaccagctt ggcgaagacc
     4681 tcgctgatct tgtgcacctc gaagaccgag cggatctcct tggactgcgg ctcgacgatg
     4741 gagcgctcgt ccgcgcggat ggccgggtcg gtggtgagcc gctccagttc ccgccggtac
     4801 tcggcgacct cgtccggcgc gatcagctgg tcgacggcga ggaagccgtc gcggtccagg
     4861 gactgcaggt cacccgccga gaccgggccg ggcgtgtccg gggagcccca gaccaccggg
     4921 tcctgccggg gggtcgccac ctcggtcgcg ccgcgggtgg gatagaggtc ggtgacgttc
     4981 gtggtcgtgg tcacggtgac tcacacctcc tcggtgagca gcgggtagac gccgttctcg
     5041 tcgtgatcct cccgtccggt gacgggcggg ttgaagacac agatgcagtg gaagtcctcc
     5101 ttgaccttga gcgtgtgccg ctcgtggccg ttgaggaggt acatcgtgcc cggcgtgatg
     5161 tggtacgtct tccccgtctc gcggtcggtc aactcggcgt cgcccttggt gcagacgacg
     5221 gcctcgatgt ggttggcgta ccacatcgac gtctccgtac ccgcgtacag gatcgtctcg
     5281 tgcagggaga agccgacctt ctccttggcg aggacgatcc gggtgctttc ccaggtgccg
     5341 gacgcgctct tcacgtgccg gtcggtgcct tcgaactcct tgaacgaacg gacgatcacg
     5401 gtgttgcgat gcctcctagg tgaaggatgt gcaaggggcc ccggcgcctt cggccgcgcg
     5461 ggcggaaggc gccgggagta tcagtggtgc ccgacggccg ggccgtcgga cggtcgagcc
     5521 ggcggtcagg ccgtctcgcg cacggcgcgg gcgagggtct tcatgccctc gtccagctcg
     5581 tcgggcgtga tggtgagcgc cggcagcagc ttgacgacct cgctctcggg gccggacgtc
     5641 tcgatgagca gcccgagctc gaaggcgcgc ttggcgacgc ggcccgcgcg gcccttgtcg
     5701 tggaactcca ggccccagac caggccgcgg ccccggtact ccttgacgtc ggcgaggttc
     5761 tcctcggtga tggcgatcat gtgctgctcg acctgctcgc cgcgcttgcg ggtctgcttc
     5821 tccatcgccg acccgtcggc ccagtacgcc tccagcgtgg cggtcgccgt gacgaaggcc
     5881 gggttgttgc cgcggaaggt gccgttgtgc tcgcccggct cccagacgtc cagctcgggc
     5941 ttgaagaggg tcagcgccat cggcatgccg tagccgctga tcgacttcga gacggtgacg
     6001 atgtccggcg tgatgcccgc ctcctcgaag gagaagaagg cgccggtacg gccgcagccc
     6061 atctggatgt cgtcgacgat gagcagcatg tcctggcgct cgcacagggc ggccagggcg
     6121 cgcagccact cggcccgggc gacgttgatg ccgccctcgc cctgcacggt ctcgacgatc
     6181 acggcggccg gcttgttcag gccggagccc tggtcctcga gcagccgctc gaaccagatg
     6241 aagtcctcga cggtgccgtc gaagtagttg tcgaacggca tgggcgtgcc gtgcaccagc
     6301 gggatgcccg cgccggcccg cttgaaggcg ttgccggtca ccgcgagcga gcccagcgac
     6361 atgccgtgga aggcgttggt gaaggacacg atcgactcgc gccccttcac cttgcgggcc
     6421 agcttcagcg ccgcctcgac ggcgttggtg cccgtcgggc ccgggaacat gaccttgtac
     6481 ggcaggtccc gcgggcgcag gatcgtgttc tggaacgtct ccaggaagcg gcgcttggcg
     6541 gtggtcgaca tgtcgagccc gtgcgtgacg ccgtcccgct ccaggtagtc gatcagcgcg
     6601 cgtttcagga cggcgttgtt gtgcccgtag ttgagcgagc ccgctccggc gaagaagtcg
     6661 aggtactcgt ggccgtcctc gtcgtacatg cggctgccga ccgcacggtc gaagacggtg
     6721 ggccagccgc ggcagtagct gcgcacctcg gactcgacgg tctcgaagac gctgaggtcg
     6781 ggctgggtga tggtcacgac gaatcgctcc tcgtggggtg agggtgggag aggaaggtcg
     6841 tactgatccg gggaccggtg cggtccgccg ggaagtctgg gggccggact ggcacggggt
     6901 gccgggccgt ccggacggcc ggtcagccgg tggcggacag cgggcctatg cggtacagga
     6961 cctcgggctc gtgcggtccg tcggggaaca cggcggtgtc gaacagcacc tcgcgctcca
     7021 cccgggcgcc gcgacgctcg gcgaacgagg tgaacagccg ctgggaggcg gtgttgtcgg
     7081 gggagatggt ggtctccagg gtggtcagcc cctgttccgc gacggtccgc tcggccagcc
     7141 cgtcgagcag ggaggcggcg agccggcgcc cgcggtacgc ctcgtccacc gccacctgcc
     7201 acacgagcag ggtgtccggc cggtccggac gtacgtatcc ggtgacgaag ccgaccggct
     7261 caccgttctc gtcgcgggcg acggccgacg tggcggcgaa gtcccggcac cagagcagat
     7321 agctgtagga ggagttgagg tcgaggacct tggaatccct cgctatccgc cacagggcgg
     7381 ccccgtcggc cactctcgga cgatcgattt gcaggtctgc ttgcgcggca gtcatgcaaa
     7441 ttgaatttac ccagggaaaa atgaaattgc atcgccggat ggggttacga atgggcgccc
     7501 cgtgtgttat cacgcgggcg cgtgcttccg cgcgagccgg cggcgttatg gggcgatttg
     7561 tagggggcgt aagccgcaaa acgggcagca tgtggatcgg gtcacaatcg cgtaacccgt
     7621 gcgagacgtg tccgaaatgt gaagatcggc gctcgtgaaa ttgttgtgtt taggtccagg
     7681 gaaagcgggc agaagaatac gggaagctgc gctgagtgga attccctaat tctgcttgtg
     7741 tgagcatcgg gaattccctc gaattcatcc cctcgtggcg tcgttcgaag agtcggccga
     7801 agcgccggtt ccggccggcg cctcccacgc gtcctcgacg gcccgccggg cggcctccag
     7861 gtcggccttg cggccccgct cggccagcgc ggcacccagt gccgccagac agccctgtac
     7921 ggccccgcgc gtcgcgtccg gaccgtagtg gttgacccgg atcatctcgc ccgccagcgc
     7981 gcccccgccc gccgccagcg gcagcgccgg gtccgactcc agggcacggg ccaccagcgc
     8041 ggaggcgtcg acgtccgccg gcgtccgcag cgtcgtcgcg accggcgccg cgtccttcgc
     8101 ctcgtacacg tacggctcca gaccgcccag cgcgagcgca ccggcccggg tcgccgccgc
     8161 ggcggacgcg tgccgcgcca tcaccgtcgc ggcgccctcc gcctcgatgc gctcgacgca
     8221 cgcctccagc gcgagcatct ccaactgcgc cggcgcgtgc agcagcgcct tgcgcccgcc
     8281 gtcgatccag cgctccttcc agtccaggag cgacaggtac gagcggcgcg gcgcgttcgg
     8341 gttcgccgcc atccgcgccc aggcccgctc gctcaccgac accgccgaca ccccggccgg
     8401 gccgcccatc gccttctgcg cgccgatcac gcacaggtcc acgccccacg cgtccggcag
     8461 caccggctcg gcgcccacgg aggccaccgc gtccaggtag aacagcgcgc cgtgcgcccg
     8521 caccacctcg ccgatctccg cgaccgggtt ggtgttgccg gtcgccgcct cggcgtgcac
     8581 cagggagacg aagtcgatct ccgggtgctc ggcgagggcc cggccgatct gctcggcgga
     8641 gacggccgtg tggaacggca ccgcgaggtc gtgcacggtc gcgccgcagt cccgcagcca
     8701 gttcccgaag gtctgcccgt acggccccgt gatcacgttc agcgcgacgg tgccgggacc
     8761 cgcggcggcg cggatcgcgc cctccagcgg cagcagcgcc tcgccctgca tgaccaccac
     8821 gtcctgcgcg gtgcccagca gacgcgcgac ccggtcctcg atcgcggcga aacggccggc
     8881 gctcagcggg gcgaggtcca ggaagggatg ggtcacggcg gtgctctttt cagtaacggc
     8941 aggtgtccgg cgcgggtacg cggcgtacgg agtcgagagt aaccatggcg cccgtcggca
     9001 ctcgcgccgg agtgcttccg ttgcgacacc cccacgcccg gaatcatggc ttccgtcaca
     9061 gccgggcggt cgccggactg atggactggc cccgtgctcg ctcttgtgcc gctggtcctc
     9121 gtcgcggtgg tgacggccgt cgtcctgcgc cggggcgccg gcgcgtaccc ccgtgcgcgg
     9181 ctgcccccgg cggctctcgc tcccgcgccg cgcaagccgg gcgcgccctt ccgcgtcgtc
     9241 gccgcggtcg cgggctgggc cgccggcctg ctctacgtct ggggcctggt gtgcgtcggg
     9301 ttcgcggcca tggaggcgga ggacggcggc acggactcag cgccgccccg gccctgccga
     9361 accggcgtgt ccccggagct gtccgggcgg gtcgtcgacc actccgtctc ctacctgccc
     9421 ctgcgcttcc gctgcgagac cgccgacggc ggggcctacg actccgccga cgtccccggc
     9481 tacgtcaatc ccggcgtcgc cgtcctcgcc ctgaccgcgg tcgcgggcgc cgtcgcggcc
     9541 ggctgggcga ccgagtcgcg cgcccgcgcg gcagcacgcg ggggcggccg gtgaccggcc
     9601 ccggacacgt gtgcgggcac cgtttagggt gcggggcatg agcgaacgcg cggtgctgca
     9661 cgtgaagggt cgggtgctcg tcgggccgga ggaggtccgg gacgaggtgt gggtgatcga
     9721 cggccggatc tcgtacgacc ggcccgccgg cgccgccgac gtccgcaccg tcgagggatg
     9781 ggcgctgccc ggcctggtcg acgcgcactg ccacgtcggc ctcgaccagc acggtgccgt
     9841 ccccgcggac gtcgccgaga agcaggcgct gaccgaccgg gaggcgggca cgctgctcat
     9901 ccgcgacgcg ggctcgccct ccgacacccg ctggatcgac gaccgcgagg acctgccgaa
     9961 gatcatccgg gccggccggc acatcgcccg cacccgccgc tacatccgca actacgcctg
    10021 ggaggtcgag cccgaggacc tcgtcgcgta cgtcgcccag gaggcccggc gcggtgacgg
    10081 ctgggtgaag ctggtcggcg actggatcga ccgcgacctc ggcgacctgt cggcctgctg
    10141 gccgcgcggc gccgtcgagg cggccatcgc ggaggcccac cggctgggcg cccgcgtcac
    10201 cgcgcactgc ttcgccgagg actccctgcg ggacctggtc gaggccggca tcgactgcgt
    10261 cgagcacgcc acggggttga ccgacgacac gatccccctg ttcgccgagc gcggggtcgc
    10321 catcgtgccc accctcgtca acatcgccac cttccccgcg ctggccgacg gcggcgaggc
    10381 gcgcttcccg cgctggtcg
//
