LOCUS       NZ_CP085300            10594 bp    DNA     linear   CON 18-JAN-2024
DEFINITION  Streptomyces sp. BSE6.1 chromosome, complete genome.
ACCESSION   NZ_CP085300
VERSION     NZ_CP085300.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. BSE6.1
  ORGANISM  Streptomyces sp. BSE6.1
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8024389)
  AUTHORS   Ramesh,C., Anwesh,M., Vinithkumar,N.V., Kirubagaran,R. and
            Dufosse,L.
  TITLE     Complete Genome Analysis of Undecylprodigiosin Pigment
            Biosynthesizing Marine Streptomyces Species Displaying Potential
            Bioactive Applications
  JOURNAL   Microorganisms 9 (11), 2249 (2021)
   PUBMED   34835376
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8024389)
  AUTHORS   Chatragadda,R. and Maile,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-OCT-2021) Andaman and Nicobar Centre for Ocean Science
            and Technology, National Institute of Ocean Technology, Dollygunj,
            Port Blair, Andaman and Nicobar Islands 744103, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 10-OCT-2021
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/18/2024 07:10:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,273
            CDSs (total)                      :: 7,198
            Genes (coding)                    :: 7,094
            CDSs (with protein)               :: 7,094
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 47 of 104
            Pseudo Genes (incomplete)         :: 80 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 30 of 104
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 01:03:07
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 5312529
            Orig. end                         :: 5323123
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP085300.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from National Institute of Ocean
            Technology.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10594
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="melanin"
                     /region_number="21"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..10594
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10594
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5594](+)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5594
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     misc_feature    695..715
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 20.42"
                     /tool="antismash"
     gene            complement(<1070..2218)
                     /locus_tag="LJA35_RS23705"
                     /pseudo=""
     CDS             complement(<1070..2218)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1045: glycosyl transferase group 1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019325465.1"
                     /locus_tag="LJA35_RS23705"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="glycosyltransferase"
                     /pseudo=""
                     /sec_met_domain="Glycos_transf_1 (E-value: 1.6e-30,
                     bitscore: 104.7, seeds: 63, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MHLPPTGLRPRVLHLTQPVDGGVARVVADLTRAQLAAGLRVAVAC
                     PDGGFADRLRALGADVRNWPATRSPGPDVVPEVRRLARVIEEVRPDLVHAHSAKAGLAG
                     RLAVRGRIPTVFQPHAWSFEAVGGAAGLLALGWERWAGRWAARTVCVSEAERLRGVRAG
                     LRGPWAVIPNGIDVTRFEADAPANAAEDPTAPTTPAGVRRRHGIAPHAPLVVCVGRLCR
                     QKGQDLLLGAWEQVLARVPGARLVLVGDGPDRARLAARAPASVLFAGTVADAAVWYRAA
                     DLVVLPSRWEGMALAPLEAMACGRPVVVTDVDGAREGLPPALVPHCLVPPGAPAALATA
                     VTALLLDAPLRTSLGHRGRAHVRSAHDVRHTAASITALYDDLL"
     misc_feature    complement(2195..2197)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            2509..2748
                     /gene="chpG"
                     /locus_tag="LJA35_RS23710"
     CDS             2509..2748
                     /codon_start=1
                     /gene="chpG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007449696.1"
                     /locus_tag="LJA35_RS23710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="chaplin ChpG"
                     /protein_id="WP_007449696.1"
                     /transl_table=11
                     /translation="MSRIAKGLALTSVAAAAVAGTAGVAAADSGAQAAAAHSPGVLSGN
                     VVQVPVHIPVNVCGNTIDVIGLLNPAFGNECEND"
     misc_feature    complement(2854..2869)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.11"
                     /tool="antismash"
     gene            3023..3748
                     /locus_tag="LJA35_RS23715"
     CDS             3023..3748
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003976096.1"
                     /locus_tag="LJA35_RS23715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_165286077.1"
                     /transl_table=11
                     /translation="MRLPARRIATSALCAGLLIGLSGPAVMAADGDSVRERTHAASRTP
                     LPDADELQSQVGSLAGLGGVLTPVTDMLTAVLKADNGQLSAADAEKLSAAVEDALAKVE
                     AADTDADDAAVTAPEAGTDTPVTLPAPVTAQNEDGTAAASDLSATAQAELQKQIDALVK
                     ATTSGTADQVSPAVEDVMTGIVNVVAATMVGSGLPAADLAGLPGVPTAPEATTPEATTP
                     DATTPEATTPEAAVPADPS"
     gene            3951..4253
                     /locus_tag="LJA35_RS23720"
     CDS             3951..4253
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007445910.1"
                     /locus_tag="LJA35_RS23720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_102931516.1"
                     /transl_table=11
                     /translation="MKSLKAAAVVAGSLVAAGSAVPAFAYDASDAAALTPTSLNGAVNA
                     LTAEPIDIMPIKHQSDARSNALDTENKDSVLHTVKGATSELNSANGLLGGLPLGG"
     gene            4365..4880
                     /locus_tag="LJA35_RS23725"
     CDS             4365..4880
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003976098.1"
                     /locus_tag="LJA35_RS23725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_233646633.1"
                     /transl_table=11
                     /translation="MKPTTRRTLAAVVTGVAAAVGAAVTPAAAAGAIPVPVPLDGAETA
                     LGMELPEVAGELPVPTAGAPEGPQYVEGRLLPERTLPQLPVHGGLPGVDLRQPVPHVLG
                     DEFDHAGVDAPAAEVRTLAPGLSLDAPLTAPNPEALGLPSPKLPEAGVLAPVVQTVPVA
                     DLGVGPGL"
     gene            5001..5594
                     /locus_tag="LJA35_RS23730"
     CDS             5001..5594
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011028525.1"
                     /locus_tag="LJA35_RS23730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="tyrosinase cofactor"
                     /protein_id="WP_233646634.1"
                     /sec_met_domain="melC (E-value: 3e-48, bitscore: 162.3,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVVDAGTRANGAEHEPRYAVRGEGAERGGPASASRRQLMRGLLVP
                     AVAVGLAPLIGASRPAGNRDAAAPAHPGPHTAGPVRDGDSAGSVRDGESAGFGDGAAFD
                     ETYRGRRIRGIRSAAGRALGAGTWHVTVDGRPLHLMRRADGSWLSMVDHYRSYPTPLAA
                     ARGAVDELGPGEHLRDTPSTGHDQHSGGRHGVHA"
     gene            5578..6444
                     /locus_tag="LJA35_RS23735"
     CDS             5578..6444
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019325459.1"
                     /locus_tag="LJA35_RS23735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="tyrosinase family protein"
                     /protein_id="WP_102931664.1"
                     /transl_table=11
                     /translation="MAYTRKDVSTLTRSEKRRFVGALLEVKRRGEYDEFVRTHIEYYVS
                     DGEDGLRTAHMAPSFLPWHRRFLLDLEEALRRVDDSVTVPYWDWTKDRGTKSTPWTADL
                     LGGTGRRSDHRVTTGPFAHAEGNWTLKENVTDTEFLTRDLGRAGNPLDLPSKSDLEWAL
                     DDPKYDTSPYDSTVRKGFRNKLEGWGTGRGSVSWRNHNRVHRWVGGAMVGGASVNDPVF
                     WLHHAFIDLQWSRWQARHRGARYLPAEPPGRGSAQRGRIVARHEKLPPWDVTPDELEDV
                     GRIYRYA"
     gene            complement(6552..6827)
                     /gene="chpF"
                     /locus_tag="LJA35_RS23740"
     CDS             complement(6552..6827)
                     /codon_start=1
                     /gene="chpF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011028524.1"
                     /locus_tag="LJA35_RS23740"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="chaplin ChpF"
                     /protein_id="WP_165286075.1"
                     /transl_table=11
                     /translation="MSRIAKAAGVALGTGAVVLSGTGMAMADAGAAGAAVGSPGVLSGN
                     VVQVPVHVPVNLCGNTIDVIGLLNPAFGNSCENGDDEGDKSGGYGG"
     misc_feature    6928..6943
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 18.82"
                     /tool="antismash"
     gene            7090..7728
                     /locus_tag="LJA35_RS23745"
     CDS             7090..7728
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007449721.1"
                     /locus_tag="LJA35_RS23745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF2087 domain-containing protein"
                     /protein_id="WP_233646635.1"
                     /transl_table=11
                     /translation="MPNDRSDTSVRASEPPSDPAQASALAALPALFAEESRVRAFAAVA
                     LGAGDTAQVARAAGLSAKEAAGALRRLRDQGVVTTGADGELAVAHDRFRELARAGRPAP
                     QAARDSGAAGDDGPAGIVVRTFVRDGRLVRLPARWTRKKVVLRHIAEQTFAPGVEYPER
                     TVDEKLRAWCEDGEVDHVTLRRYLVDLGHLCRSEGVYCRPVDSDRAGTA"
     gene            complement(7736..8722)
                     /locus_tag="LJA35_RS23750"
     CDS             complement(7736..8722)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) 2-Hacid_dh_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1072: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007449723.1"
                     /locus_tag="LJA35_RS23750"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="WP_233646636.1"
                     /sec_met_domain="2-Hacid_dh_C (E-value: 8.4e-52, bitscore:
                     173.8, seeds: 135, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRLRCAVLDDYQRVATEVADWSPVADVVDVVPHDTHFPDEDALAA
                     ALADADIVVTLRERVPFPGSLIARLPRLKLIVASGMRNSVIDYAAAEAHGVTVCGTASS
                     STPPVELTWALLLGLARGIVQEANGVREGGPWQRTVGADLHGRRLGLLGLGKIGGRVAQ
                     VGLAFGMEVSAWSQNLTRERADEVGVELAPSKEELLRSADFVSVHLALGERTRGLLGPA
                     ELALLKPTAYLVNTSRAAIVDQEALLAALHEGRIAGAGVDVFDTEPLPAGHPMRTAPRL
                     LATPHLGYVSRANYATYYGQAVEAIQAYLAGSPVRRLPVPAAAPSPV"
     gene            complement(8796..9233)
                     /locus_tag="LJA35_RS23755"
     CDS             complement(8796..9233)
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /locus_tag="LJA35_RS23755"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_134652961.1"
                     /transl_table=11
                     /translation="MGNYAWSHRLEDVDLEELSRLYRIAPLGDKPPERLATVFGNSRFT
                     CFVRAGDTLVGAGRALADGADCAYIADVAVHPDHQGQGLGSDIIRRLLELARDHKKILL
                     YASPGTEPFYRKLGFLPMNTAMAVWADPDRAVDIGLLRREP"
     misc_feature    complement(9277..9293)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 14.92"
                     /tool="antismash"
     gene            9355..10569
                     /locus_tag="LJA35_RS23760"
     CDS             9355..10569
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1184: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /locus_tag="LJA35_RS23760"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MFS transporter"
                     /protein_id="WP_233646637.1"
                     /transl_table=11
                     /translation="MSKTSAAPGASAPGNAGAPRTSRVLPVMTLAAGLSVAGNYFAQPL
                     LGLLRTELHMTTTTAGMVVAVSQIGYALGLAFLVPLGDRYPRRDLAASLLAATGVLLLL
                     AGVAPNGPLLLAATGLVAVTSVGAQVLVPFAAELSAPDSRARNVGTVMAGVLAGGLIGR
                     AFSGALADVAGWRSVYWVTAVLLLATAFAVRRVLPESRGNRTDETRPGSLLRSTAALLV
                     EVPALRRPVAVASLAMAGYTVHLVTITLLLVEEPYDWSTSTIGAVGLVGAVGPLFMPLA
                     GRYVDRGHPRAVLLAGLLLSGLAWLVMLPARNGEVGWLIIGMIMINLGQTAMLNASQNT
                     CYEVRPEARSRINAVFMTLFFAGGALGGALAPPVWSAYGWNGVCALAGAIAAVGLIAAA
                     RPYRF"
ORIGIN
        1 ggcaggtcgt ccagcgcacc ggacggccgc cggcccggac gcgggcgggg gcgcagcagc
       61 agcgaagcgg ccaccagcac gcccgtgagc agcggttggt gcggggcacc ggtgaggacc
      121 agcgtgccgg gcacggccgc cgttccgtcc acgacgagca gcggcagcgg cgaacccggc
      181 cgcggtgcgg gccgcccgga gggcagccga gggctcccgg tgccgtcgcg ctggggcagc
      241 accgcgacgg gggaggagcc gaggtcgcgc gactggccgg cgggggaggg gacggtgctt
      301 tccgcggtca cgggtgggtg gactccctgc actcgatggg cacgacggcc gaccggcccg
      361 cggctgcgag ccggtcgccg gggaggttgc gcggcacggc ggacggacct gcgccgtcgc
      421 caagtccgcc gacgaccacg acggcgcaca ccgcgccccc gacccccgcc ccggccgcgc
      481 accctgcgcc gacggtgatt cctacgccgg ccgtagacct cgcgccggcc gcgggcctcg
      541 cgttggttgc gggcctggcg tcgactgtgg gttccgcgcc ggctgcaggt ctcgccttgg
      601 tggtggtcct tgcgtcagcc gcgggtcccg cgccggttgc aggccccgca cctgctgtgg
      661 gccctgcgtc gactgtgggt tccgcatcgg ccgcaggcct cgcactggct gtgggcctcg
      721 tgccggccgc gggccccggg ttggttgtgg gccttgcgtc gactgtgggt tccgtgccgg
      781 cagcaggtcc ctcgttggtc gtgggcctcg tgtcggccga aagccccgca ccggccgcgg
      841 gcctcgcacc ggccgttggc cccggggcgg ccatgcgtcc cgtgacggtc gcaggacccg
      901 ggttgactgt gggccctgcg gcgactgtgg gtcccgtgcc ggtcgcaggt ctcgtactgg
      961 ctgtgggcct cgcgtcggcc gcaggccccg caccggccgt tggccccggg gcggccgtgc
     1021 gtgccgtgac ggtcgcaagc cacccaccgg ccgtgggtcg tgcggcgtcg aggaggtcgt
     1081 cgtacagggc cgtgatcgac gccgccgtgt ggcgtacgtc gtgtgccgag cggacgtgcg
     1141 cgcgcccccg gtggccgagc gacgtgcgca gcggcgcgtc gaggaggagc gcggtgacgg
     1201 cggtggccag ggcggccggg gccccgggcg ggaccaggca gtgcggtacg agcgcgggcg
     1261 gcaggccctc gcgggcgccg tccacgtcgg tcacgaccac gggccggccg caggccatcg
     1321 cctccagcgg cgccagcgcc atgccctccc agcgcgaggg caggacgacc aggtcggcgg
     1381 cccggtacca gacggcggcg tcggccacgg tcccggcgaa gagcacggat gccggggccc
     1441 gcgcggccag ccgggcgcgg tccggtccgt caccgacgag gaccagccgg gcgccgggca
     1501 cccgggcgag aacctgctcc cacgcgccca gcaggaggtc ctgccccttc tgccggcaca
     1561 gccggcccac gcacaccacc aacggcgcgt gcggagcgat gccgtgccgc cgcctcaccc
     1621 cggccggggt ggtcggggcg gtcgggtcct cggcggcgtt cgcgggcgcg tcggcctcga
     1681 agcgggtcac gtcgatgccg ttcgggatga ccgcccaggg tccgcgcagc ccggcccgca
     1741 caccccgcag ccgttccgcc tcgctcacgc acaccgtccg tgccgcccac cgcccggccc
     1801 accgctccca gccgagcgcg aggagcccgg cggcaccgcc gaccgcctcg aacgaccagg
     1861 cgtgcggctg gaacacggtc gggatccggc cccgtacggc gagccgcccg gccagaccgg
     1921 ccttcgcgct gtgcgcgtgc accaggtcgg gccgtacctc ctcgatgacg cgggcgagcc
     1981 gccgtacctc cggcaccacg tccggacccg gcgagcgggt ggcgggccag ttccgcacgt
     2041 cggcgccgag cgcacgcagc cggtcggcga agcccccgtc ggggcaggcg acggcaacgc
     2101 gcagtccggc ggcgagctga gcgcgcgtca ggtccgccac cacccgggcg accccgccgt
     2161 ccaccggttg ggtgaggtgc agaacgcgtg gccgtaagcc ggtcggtggc aggtgcatgc
     2221 gcgtttcctc gctcacggag aatgctcggg acggcgggaa cgcgcccgac cgcggggcgc
     2281 cggtcgcgac gcggcatacg ggtgcggcat acgggtgcgt cgaacggtgt gggactctag
     2341 ctgctatccg tactaatacg gcgaaaccgg gtaagtgtgt tggatttgtg aatcccgcgt
     2401 ttttgcgaga tcaccccttt ggcggcacaa ctcggggccc tgccacgcgt tgacactgcg
     2461 ccgggcagcc gcccggatgt gtgtgacaac cccaaggagc acttctccat gtcgcgtatc
     2521 gcgaagggcc tggccctgac ctccgtcgcc gccgccgcgg tggcgggcac cgccggtgtc
     2581 gccgccgccg acagcggcgc gcaggccgcc gccgcccact ccccgggcgt cctgtccggc
     2641 aacgtggtgc aggtcccggt ccacatcccg gtcaacgtct gcggtaacac catcgacgtc
     2701 atcggcctgc tgaacccggc gttcggcaac gagtgcgaga acgactgacg tcggctggca
     2761 actctccgca ccggccgtcc gcaccgcggt tcctccgcga gcgggcggcc ggttcgcgtt
     2821 gccgtgcgga cggcgtccgc tgccccgaat ggggggaccg cgcggtgaca cggcgcacgg
     2881 accttgacag ggcgtctcag caggcaggac gccgagcggg ggaacgacgg gcgggccggc
     2941 cgggtcaccg ctcggttgca gagcgccagg ggcacttcca cggtgggcac aacatccgac
     3001 gcaccaccga aaggaacccc ccatgcgtct gcccgcacgg cgaatcgcca cctccgctct
     3061 ctgcgccggc ctgctgatcg gcctctccgg tccggcggtg atggcggccg acggcgactc
     3121 ggtccgggaa cgcacccacg cggcgtcccg cactcccctg cccgacgccg acgaactgca
     3181 gagccaggtc ggcagcctgg ccgggctggg cggagtgctc acgcccgtca ccgacatgct
     3241 caccgcggtc ctcaaggccg acaacggcca actctccgcc gcagacgcgg agaagctctc
     3301 cgccgccgtc gaggacgccc tcgccaaggt ggaggccgcg gacacggacg ccgacgacgc
     3361 ggccgtgacc gcgccggagg ccgggaccga caccccggtc acgctgccgg cgcccgtcac
     3421 ggcgcagaac gaggacggca ccgcggccgc ctccgacctg tccgcgaccg cgcaggccga
     3481 gctgcagaag cagatcgacg ccctggtcaa ggccaccacc tcgggtaccg ccgaccaggt
     3541 gagccccgcc gtcgaggacg tgatgaccgg catcgtcaac gtcgtcgccg ccacgatggt
     3601 cggcagcggg ctgcccgccg cggacctggc cggcctgccc ggcgtgccca ccgcgccgga
     3661 ggccacgact cccgaggcca cgactccgga cgccacgact ccggaggcca cgactcccga
     3721 ggcggccgta ccggcggacc cctcctgacg ccgagccgcc cccgggcgtc ccgggccgtc
     3781 gcacctcccg tcgtggaggc gcggcggccc tttccgtgtc cgggggcctc atccgggtcc
     3841 ggagatctca tatgtcttcc ggacggcgga gtttccgggt ccggcgcagg tcgttaggga
     3901 gggcgtcagc aatcccaccg gtgacacggg tcgccgagga aaggaacacg atgaagtccc
     3961 tcaaggccgc cgccgtcgtc gccggttccc tggtcgccgc cggttccgcc gtgcccgcct
     4021 tcgcctacga cgcctccgac gccgccgccc tcacgcccac cagcctcaac ggtgccgtga
     4081 acgcgctgac cgcggagccc atcgacatca tgccgatcaa gcaccagtcg gacgcgcggt
     4141 ccaacgccct ggacaccgag aacaaggact ccgtgctgca caccgtgaag ggcgcgacct
     4201 ccgaactgaa ctccgcgaac ggcctgctgg gcggtctccc gctgggcggc tgaccgccgt
     4261 cccgcgccct cgcccctcgc cgcaccccct cgtcccgcct tcccgccttt ccgtcctccc
     4321 ggaaagccgt acccccgcct tctcgaaagg gacccgtgcc tgtcatgaag ccgaccacca
     4381 gacgaaccct ggccgccgtc gtcaccggtg tggccgccgc cgtgggcgcg gcggtgactc
     4441 ccgccgccgc ggccggcgcg atccccgtgc cggtgccgct ggacggtgcg gagacggccc
     4501 tcggcatgga actgcccgag gtggccggcg agctgcccgt gccgaccgcg ggcgccccgg
     4561 agggcccgca gtacgtcgag ggccggctgc tgccggagcg gacactgccg cagctgccgg
     4621 tgcacggcgg gctgcccggc gtggacctgc gccagccggt gccgcacgtc ctcggggacg
     4681 agttcgacca cgccggcgtc gacgcgcccg ccgccgaggt gcgcaccctg gcgccgggcc
     4741 tgtccctgga cgccccgctc accgcgccga accccgaggc cctcggcctg ccgtcgccga
     4801 agctgcccga ggccggcgtg ctcgccccgg tggtccagac ggttccggtg gccgacctgg
     4861 gcgtgggacc gggcctgtag cccccgcggc gaccccgctt ccgggagcgg ccgtgctccg
     4921 actggccctg cgtacggtga ccgcggggcc cgtcgtgcgg ttagcggtgc ggaccgacgc
     4981 aaccggacga ggagtgagcc atggtggtcg acgcgggtac gagagcgaac ggcgcggagc
     5041 acgagccgcg gtacgcggtg cggggcgagg gggcggagag aggcgggccg gcgtccgcct
     5101 cccggcggca gctgatgcgg gggctgctcg tgccggccgt cgccgtcggt ctggcgccgc
     5161 tgatcggcgc ctcccggccc gccgggaacc gggacgcggc ggcgcccgcg catcccgggc
     5221 cgcacaccgc cggacccgtc cgggacggtg attccgccgg gtccgtccgg gacggcgagt
     5281 ccgccgggtt cggcgacggt gccgccttcg acgagaccta ccgcgggcgg cgcatccggg
     5341 gcatccgctc cgccgcgggg cgggccctgg gggccggcac ctggcacgtc accgtggacg
     5401 gccgcccgct gcacctgatg cggcgggccg acggcagctg gctgagcatg gtcgaccact
     5461 accggtcgta ccccaccccg ctcgccgctg cccgcggcgc cgtcgacgaa ctcggccccg
     5521 gcgagcacct gcgcgacacc ccgtcgaccg gccacgacca gcactccggg gggcggcatg
     5581 gcgtacacgc gtaaggacgt cagcacgctg acccgcagcg agaagcggcg gttcgtgggc
     5641 gcgctgctgg aggtcaaacg acgcggcgag tacgacgagt tcgtgcgcac ccacatcgag
     5701 tactacgtct ccgacggcga ggacggactg cgtacggcgc acatggcgcc gtccttcctg
     5761 ccctggcacc gccggttcct gctggacctg gaggaggcgc tgcgccgggt ggacgactcg
     5821 gtcaccgtgc cgtactggga ctggacgaag gaccgcggca ccaagtccac tccctggacc
     5881 gccgacctgc tgggcggcac cgggcggcgc tccgaccacc gggtcaccac cggccccttc
     5941 gcccacgccg agggcaactg gaccctcaag gagaacgtca ccgacaccga gttcctcacc
     6001 cgggacctcg gacgcgccgg caaccccctc gacctgccct cgaagagcga cctggagtgg
     6061 gcgctggacg acccgaagta cgacacctcg ccgtacgact ccacggtccg caaggggttc
     6121 cgcaacaagc tggagggctg ggggaccggg cgcggcagcg tctcctggcg caaccacaac
     6181 cgggtgcacc gctgggtcgg cggggccatg gtcggcggcg catccgtcaa cgacccggtg
     6241 ttctggctgc accacgcctt catcgacctg cagtggtccc gctggcaggc ccgccaccgc
     6301 ggcgcccgct acctgcccgc cgagccgccc ggccggggca gcgcgcagcg cgggcggatc
     6361 gtcgcccggc acgagaagct gccgccgtgg gacgtgacgc cggacgagct ggaggacgtg
     6421 ggccggatct accggtacgc ctagacgggt ccgatcccgg gtgggccgac cccgggtggg
     6481 ccgagcacct ccggcccgga aacggcggga gccccggccg ggtgggccgg ggctcctcgg
     6541 tgcctggcga ctcagccgcc gtagccgccg ctcttgtcac cctcgtcgtc accgttttcg
     6601 cagctgttgc cgaaggccgg gttcagcagg ccgatgacgt cgatggtgtt gccgcagagg
     6661 ttgaccggca cgtgcaccgg gacctggacg acgttgccgg acaggacacc gggcgagccg
     6721 acggccgcgc ccgccgcgcc cgcgtccgcc atggccatgc cggtgccgct gagcaccacg
     6781 gcaccggtgc cgagggcgac accggcggcc ttcgcgatgc gagacatcac gttctccttc
     6841 tgactcgggg agcgcggcgg caggacgcgc cgccgcactg ccggttcaac gcggccggtc
     6901 gcagccggtc acggggagaa tgtacggatc accctttttg gacatgtggg ccggtggcga
     6961 cgcggacggc cttcggtggg cgggcggatg gtcttcggcg ggcgagcggg cagtctccgg
     7021 cggcgggcgg gcagcctccg gcgggaaaac cggtgccgtg cgcgcccgtg ggtccactag
     7081 cctcccggca tgccgaacga ccgatccgac acgtccgtca gggcttccga gccgccgtcc
     7141 gatcccgcgc aggcctccgc actggccgcg ctgcccgccc tgttcgccga ggagagccgg
     7201 gtgcgggcct tcgccgccgt ggcgctcggc gcgggcgaca ccgcacaggt ggcccgggcg
     7261 gccggcctca gtgcgaagga ggcggccggt gcgctgcgcc gactgcggga ccagggcgtg
     7321 gtgacgaccg gcgccgacgg ggaactcgcc gtcgcccacg accgtttccg cgagctggcc
     7381 cgggccggcc gcccggcgcc gcaggcggcc cgggactccg gtgcggcggg cgacgacggc
     7441 ccggccggca tcgtcgtacg gaccttcgtc agggacggcc ggctggtccg gctgcccgcg
     7501 cgctggacgc gcaagaaggt ggtgctgcgg cacatcgccg agcagacctt cgcacccggc
     7561 gtggagtacc cggagcgcac cgtcgacgag aagctgcgcg cctggtgcga ggacggcgag
     7621 gtcgaccatg tgacgctccg gcgctatctg gtggacctcg gtcacctgtg ccggagcgag
     7681 ggcgtctact gccgcccggt ggactccgac cgggcgggca ccgcctgagc agtccctaca
     7741 ccgggctcgg tgcggccgcg ggcacgggca ggcgccgcac gggggagccc gcgaggtagg
     7801 cctggatcgc ctcgacggcc tggccgtagt acgtggcgta gttggcgcgc gacacatagc
     7861 ccaggtgggg ggtcgcgagc aggcgcgggg cggtgcgcat agggtggccg gcgggaagcg
     7921 gctcggtgtc gaagacgtcc actcccgcgc ccgcgatgcg cccctcgtgc aacgcggcga
     7981 gcagcgcctc ctggtcgacg atcgccgcgc gcgaggtgtt gaccaggtag gcggtcggct
     8041 tgagcagggc gagttcggcg gggccgagca ggccgcgggt gcgctcgccg agcgccaggt
     8101 ggacggagac gaagtcggcg ctgcgcagca gctcctcctt ggacggggcc agttcgacgc
     8161 cgacctcgtc ggcgcgctcc cgggtgaggt tctggctcca ggcgctcacc tccatgccga
     8221 aggccaggcc gacctgggcg acccggccgc cgatcttgcc caggcccagc agcccgagcc
     8281 ggcggccgtg caggtcggcg cccaccgtcc gctgccaggg gccgccctcg cgcaccccgt
     8341 tggcctcctg gacgatgccg cgggccaggc cgagcagcag agcccaggtc agctcgacgg
     8401 gcggggtcga ggaggaggcc gtaccgcaga cggtgacacc gtgggcttcg gcggcggcgt
     8461 agtcgatcac gctgttgcgc atgccggagg cgacgatgag cttcagccgg ggcagccggg
     8521 cgatcaacga gccggggaac gggacgcgtt cgcgcagggt gaccacgatg tcggcgtccg
     8581 ccagagccgc ggcgagggcg tcctcgtccg ggaagtgggt gtcgtgcggg acgacgtcca
     8641 ccacgtcggc caccggcgac cagtcggcga cctcggtcgc cacccgctgg tagtcgtcca
     8701 gtacggcaca gcgcagtcgc acggtcgtcg gtccctctcg ccgaaggggc agtggcgggc
     8761 gccgagtcta ccgacctccg gggccccggg gtctgtcagg gctcccggcg gagcagaccg
     8821 atgtcgaccg cgcggtccgg gtcggcccag accgccatgg cggtgttcat cggcaggaag
     8881 ccgagcttcc ggtagaaggg ttcggtgccg ggactggcgt agaggaggat cttcttgtgg
     8941 tcccgcgcca gctccagcag cctgcggatg atgtcggacc cgaggccctg tccctggtgg
     9001 tccgggtgga cggcgacatc ggcgatgtag gcgcagtcgg cgccgtcggc cagtgcccgc
     9061 ccggcgccga cgagcgtgtc accggcgcgg acgaagcagg tgaagcggct gttgccgaac
     9121 accgtggcga gccgctcggg gggtttgtcg cccagcggtg cgatccggta gaggcgcgac
     9181 agctcctcca ggtccacgtc ctcgaggcgg tgactccacg cgtagttccc caacgtcctg
     9241 ccgtcccttc ccgtttcgtg ctgccgagcc ttcccatttt catctcgtta cgagatcgat
     9301 cctatgagaa gaatgagttg gacgcatggg taaccggtct tcgaacatgg ggccatgtcg
     9361 aaaacctccg ccgccccggg tgcgtcggct cccgggaacg ccggcgcgcc gcgcacgtcg
     9421 cgcgtcctgc cggtgatgac cctggccgcg ggcctgtccg tcgccgggaa ctacttcgcg
     9481 cagccgctgc tcggcctcct gcgcaccgag ttgcacatga ccacgaccac cgcgggcatg
     9541 gtggtcgccg tctcccagat cggttacgcg ctcggactgg ccttcctcgt cccgctcggc
     9601 gaccgctatc cgcgccggga cctcgcggcg tccctgctgg ccgccaccgg cgtcctgctc
     9661 ctgctggcgg gcgtcgcccc caacgggccc ctgctgctgg ccgccaccgg actcgtcgcc
     9721 gtcacctccg tcggcgccca ggtcctcgtg cccttcgccg ccgagctgag cgccccggac
     9781 agcagggcgc gcaacgtcgg caccgtgatg gccggagtcc tcgccggcgg gctgatcggc
     9841 cgcgccttct ccggcgccct ggccgacgtc gccgggtggc gttcggtgta ctgggtcacc
     9901 gccgtactgc tgctggccac cgccttcgcc gtgcggcgcg tactgccgga gtcccggggc
     9961 aacaggaccg acgagacccg cccgggcagc ctgctgcgct cgaccgccgc cctgctcgtc
    10021 gaggtgccgg cgctgcgccg gcccgtcgcc gtcgcgtcgc tggccatggc cggctacacc
    10081 gtccacctgg tcacgatcac gctgctgctg gtcgaggagc cctacgactg gagcacgtcg
    10141 accatcggcg cggtcggact cgtcggtgcc gtgggcccgt tgttcatgcc cctggccgga
    10201 cggtacgtcg atcgcgggca cccgcgcgcc gtcctcctgg ccggactgct gctgtcgggc
    10261 ctggcgtggc tggtgatgct gccggcgcgg aacggggagg tcggctggct catcattggc
    10321 atgatcatga ttaacctggg gcagacggcc atgctgaacg cgtcccagaa cacctgctac
    10381 gaggtgcggc ccgaggcgcg cagccgcatc aacgccgtct tcatgaccct gttcttcgcc
    10441 ggcggcgccc tcggtggcgc cctcgccccg ccggtctggt ccgcctacgg gtggaacggg
    10501 gtgtgcgcac tggcgggcgc catcgccgcg gtgggcctga tcgccgccgc ccgtccctac
    10561 cgtttctgac cgttcctgag acccctcccc gccg
//
