LOCUS       NZ_CP085300            10294 bp    DNA     linear   CON 18-JAN-2024
DEFINITION  Streptomyces sp. BSE6.1 chromosome, complete genome.
ACCESSION   NZ_CP085300
VERSION     NZ_CP085300.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. BSE6.1
  ORGANISM  Streptomyces sp. BSE6.1
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8024389)
  AUTHORS   Ramesh,C., Anwesh,M., Vinithkumar,N.V., Kirubagaran,R. and
            Dufosse,L.
  TITLE     Complete Genome Analysis of Undecylprodigiosin Pigment
            Biosynthesizing Marine Streptomyces Species Displaying Potential
            Bioactive Applications
  JOURNAL   Microorganisms 9 (11), 2249 (2021)
   PUBMED   34835376
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8024389)
  AUTHORS   Chatragadda,R. and Maile,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-OCT-2021) Andaman and Nicobar Centre for Ocean Science
            and Technology, National Institute of Ocean Technology, Dollygunj,
            Port Blair, Andaman and Nicobar Islands 744103, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 10-OCT-2021
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/18/2024 07:10:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,273
            CDSs (total)                      :: 7,198
            Genes (coding)                    :: 7,094
            CDSs (with protein)               :: 7,094
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 47 of 104
            Pseudo Genes (incomplete)         :: 80 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 30 of 104
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 01:03:07
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4697727
            Orig. end                         :: 4708021
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP085300.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from National Institute of Ocean
            Technology.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10294
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="19"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10294
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10294
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5294](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5294)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(570..2081)
                     /locus_tag="LJA35_RS21015"
     CDS             complement(570..2081)
                     /EC_number="2.4.-.-"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010469705.1"
                     /locus_tag="LJA35_RS21015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="WP_233646420.1"
                     /transl_table=11
                     /translation="MTRRTAVLRLSVLRPSVLRRRVPLWVVPLLWTSALGLWGLTRRHS
                     VWRDEAATWQVARRSAGEIWHMLGQVDAVHGLYYLFMHALFECFGPGVVVLRLPSVLAV
                     AVAAACTTVIGRRLAGARVGLTAGLALGLLPAVQFYLQEGRPYALVMVGAALSTLLLVR
                     VLRGHGRTAHWVAYGGIVLVCALLNWLSLLLLPAHGTTLMWVRAERRVWARWSTAAAAA
                     TVGALPLILFSRTQAGQVSWIPPLTWHMLIGPAVLLAVGGLGARLDRPRVGRPSVAAVG
                     LPLLAVPQLGLLGLSLVQPLFLDRYILFSMVGLTLLIGAAVDAVVRAAGSRFPRAVPWL
                     VPVVVAVAVVALLPQSLAKRSPSSRVDDVLSAAVSVRHLKQDGDAVVFVPAARRDTELV
                     SPDAFAGLRDIALARGPAASGTLKGEEADPERIRTRMLEQRRILLVTDAPGVARPPAAR
                     RDVVKYAVLREDFDVVADEQARGRRVTVYERRAPGPSRDRAARAE"
     gene            complement(2078..2722)
                     /locus_tag="LJA35_RS21020"
     CDS             complement(2078..2722)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006374867.1"
                     /locus_tag="LJA35_RS21020"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_233646421.1"
                     /transl_table=11
                     /translation="MIDIVSLSKTFGPRTLWSGLSTTVGKGEMLALVGPSGAGKSTLLN
                     CIGLLDKPNSGEIRFEGEDITRFGRRDTRRFRRETLGYLFQNYALMENATVRENLEIAT
                     RPRRSARGRANSDIAEALDRVGLAGRGDEKVHHLSGGEQQRVALARLIVKEPALVLADE
                     PTGALDHANATAVVDLLREMSEAGCTVVVATHDDRVKDRCDAVFTVGAARS"
     misc_feature    complement(2695..2715)
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 23.22"
                     /tool="antismash"
     gene            complement(2777..4897)
                     /locus_tag="LJA35_RS21025"
     CDS             complement(2777..4897)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="LJA35_RS21025"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_233646422.1"
                     /transl_table=11
                     /translation="MLHRGIKFVHVAVLLFSAILSFLFVRGLDEELVLGSSAQVWVTGS
                     DDSVSGGQIAGAIAEFADHHEVAVARALPDVKRPDRLRHLYLASGDSASDAEAWLTDGY
                     PAFGSHVDTAVHPLAEIGQRDPRGFYYVFGPPGAADDLVARFADLGLSATVQHPLAPGA
                     LARGFTEGELFWSLGVVALAAVTMTGASVLLSAKTYAVLRLQGISLADLLLRDLRQLAV
                     FWAAAAAAVTATALTCLGLYNGLAHLGLFASVAVCLAGLLVLLVVAAHAAALALTFKVD
                     VLRALKGELPARTAFAVTYLVRVPAVLLALGFAMDVVSAGQHVAARQNNQDVYAKAGDA
                     VTIRLNGSWGGDSAHVVTAVGRWLRKADAAGEIVVAGRHDLRNLPSNARLPQGEMLVVN
                     ESFLAEQRVVDPEGRPYAPLPHDGEGAADAHTVRLLVPQSLAHHASAIRAAVPDVFGRL
                     DPEVRGRLTVETLPAKSGQRVFGYNSGDRSPNAAYTPDDDRTLVRDPVLVVVPNGSRIL
                     TDDAYTTFATQDAIVFPDPGDVLGPLHANDHDLQTYVATVNPVGQNAAESLRSAVNDFR
                     LLFFNLLASLAVLLITGIGVCVIHSRKNAQAAFAQYLNGWRFTATHRFILGVEGVLAAF
                     LAGWVPIQTWQQNRDLEELAARGIPAPFPAVHLTGLDLGVIAGLVVVEFGAVLLALGVF
                     HRRTVKERAAEA"
     misc_feature    complement(4868..4882)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 17.3"
                     /tool="antismash"
     gene            complement(5001..5294)
                     /locus_tag="LJA35_RS21030"
     CDS             complement(5001..5294)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Lactococcin_972"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR01653.1"
                     /locus_tag="LJA35_RS21030"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_102930313.1"
                     /sec_met_domain="Lactococcin_972 (E-value: 2.7e-16,
                     bitscore: 58.6, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLSLKNSIKAVAATGVVVMAFAAPALAAEDAGGGKWSHGVGANYV
                     YSNYYHKDNYHSSSVEGAYWAKSGCTKEKVWSKASAKKSTWALNKAYYDPAC"
     misc_feature    complement(5364..5377)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.97"
                     /tool="antismash"
     misc_feature    5528..5546
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 22.62"
                     /tool="antismash"
     gene            complement(5818..6102)
                     /locus_tag="LJA35_RS21035"
     CDS             complement(5818..6102)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="LJA35_RS21035"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_146045890.1"
                     /transl_table=11
                     /translation="MRKLIALSPLLTAVVAATLSVGVAAPAQAANGWNATVQCTKFRIE
                     DSSGHGFFNGHGWAKTKDLAWKAAIKDANDQMPRGYRAKHCVKKKIVRG"
     misc_feature    6087..6101
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 17.49"
                     /tool="antismash"
     gene            6581..7369
                     /locus_tag="LJA35_RS21040"
     CDS             6581..7369
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007388771.1"
                     /locus_tag="LJA35_RS21040"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_233646423.1"
                     /transl_table=11
                     /translation="MTNPTTPTAAPVTTIGARLRVALPVDRWEATGFPGRWVGGTAMTL
                     GPLLMLTGALLRVRFPFFFPDQLAAYERHPTLMATAYGLFAAGGLLLWPAVAVLAARIG
                     VRSAGWGLWGGVLVMFGLFARAFHAGVDHLAFQLVRSQGAGPATEAVSAGYGAFHVFAT
                     LNLAILAGWIVLALGAWRTRVLGPARAAALGLTSALPLGVLKGTTPLSLVALAGLCAAL
                     VPLGLALLREAPRPSRAAVLRWVPLTLVTLGLMVLLGQAG"
     gene            7397..8551
                     /locus_tag="LJA35_RS21045"
     CDS             7397..8551
                     /GO_function="GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1048: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007388772.1"
                     /locus_tag="LJA35_RS21045"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="sensor histidine kinase"
                     /protein_id="WP_233646424.1"
                     /transl_table=11
                     /translation="MKPRRQRHLHALDLGAALALTAVYIGFARLTAEDGQPVYTGPFWL
                     GCLIAALVGLPVAVRRRWPLAVLAAVLTALAVASLLDIPREPYTAAGLAAYLVGLAEPA
                     RRSVPALAVTLPVACGAVYVGEAVVTPAEDWRGAAGVAGLVLLVIGGAWSTGFTVRGRR
                     AEAAGEQRRRSERALDEERLRIARELHDIVSHKLSLIAVKAGVAGHVAEADPREARAAL
                     KVIEETSRSALAEMRRTLGVLRTEGAPLGPVPGLDRLDLLAEEAHRAGVEVDLSVHAGD
                     GLAEGTQVTIYRIVQEAVTNVVRHAAPTRCRVTIEVDAPEVRIDVVDEGPPPAHRPAVT
                     GLPGGHGLLGMRERAMMYGGSFEAGPRPEGGFAVSVRLPTEGNR"
     gene            8548..9240
                     /locus_tag="LJA35_RS21050"
     CDS             8548..9240
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1016: LuxR family
                     DNA-binding response regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007388773.1"
                     /locus_tag="LJA35_RS21050"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="response regulator transcription factor"
                     /protein_id="WP_233646425.1"
                     /transl_table=11
                     /translation="MTTTDATPVRVLIADDQAMVRGSFRVLVDHTPGMTAVGEAANGAE
                     AVELARRERPDVVLMDIRMPDLDGIEATRQICTDPALPAVRVLILTTFDLDEYVYAALR
                     AGAAGFLLKDTPPSEVLTAIGVVASGEALLAPSVTRRLIAEFARRPEPARPPSRSLDGI
                     TDRELEVLGLIARGLSNTELAEHLHLSLATVKTHVGRLLTKLRARDRAQLVIVAYETGL
                     VGDRRRGV"
     gene            complement(9259..9693)
                     /locus_tag="LJA35_RS21055"
     CDS             complement(9259..9693)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF042490.2"
                     /locus_tag="LJA35_RS21055"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF6650 family protein"
                     /protein_id="WP_233646426.1"
                     /transl_table=11
                     /translation="MGIRPTAFSAFGFGVNWEFTKSDRAIAQEVMDFLADKRVLTIGYA
                     RPQSEAAACLASAAECRTQLSEYLTQLKKPGSDLRIWLRAMRQAFTDFIEAGGSNGGRF
                     GPGSDRRFNEALAQLRDRVQQQADEVSVRYKLGGLNLPER"
ORIGIN
        1 cgccgggcca ccggtctgcg cacctgggag acccacaccc tgcgccacgg cgactccacg
       61 gtccgcgtca cctcgctccc cggccgccac gcgcccggcc ccgcccgctt cctgctgccg
      121 ccggtgatgg gcagcctgct ggagttcggc gacggcacgg gcgagacccg gctcgtcgtc
      181 tacctctccg gcgacaccct gatgtacgag ggactgcgcg agatcgccgt acgccacccg
      241 gacatccacc tggccgtcct gcacctcggc ggcacgaccc tgcccggcgg tctcgtggtc
      301 accatggacg ccgaacaggg cgccgacctg ctcgacctcc tgcgcccgcg ccgggccatg
      361 cccgtccact acgacgacta cacggtcttc cgctccccgc tcgacgactt cctcacggag
      421 gccgaccgcc ggggccacgc cgcccgcctg ctgcggtgcg ccccgggcga gcacgtgacg
      481 gtgggcgcgg aggcaggggc ctgacggagc cgagccgtcg aggggggcgg gagcggccgg
      541 ttggagccgg cgccggaggc cggatcgcgc tactcggccc gtgccgcgcg gtcgcgggac
      601 ggacccggag cgcgccgctc gtacacggtg acccgccgtc cgcgggcctg ttcgtccgcg
      661 accacgtcga agtcctcccg cagcaccgcg tacttcacga cgtcgcgccg cgcggccggg
      721 ggccgggcca cgccgggcgc gtcggtgacc agcagtatgc gccgctgttc gagcatccgc
      781 gtccgtatcc gctccgggtc ggcctcctcg cccttcaggg tcccggacgc cgcgggaccg
      841 cgcgccagcg cgatgtcccg aaggccggcg aaggcgtcgg gggagaccag ctcggtgtcg
      901 cgccgggccg ccggtacgaa gacgaccgca tccccgtcct gcttgaggtg ccgtacgctc
      961 acggccgccg acaggacgtc gtccacgcga ctggacggcg accgcttggc cagggactgc
     1021 ggcagcaacg ccaccacggc caccgcgacg acgacgggta cgagccacgg caccgcacgc
     1081 gggaagcggg atccggccgc gcgcaccacc gcatccaccg ccgcgccgat cagcagggtg
     1141 agccccacca tgctgaacag gatgtatcgg tcgaggaaga gcggctggac cagggacagg
     1201 ccgagcagcc ccaactgcgg taccgcgagc agcggcaggc cgaccgcggc caccgacggc
     1261 cggcccacgc gcggccggtc cagccgggct ccgagaccgc cgacggccag caggaccgcc
     1321 gggccgatca gcatgtgcca ggtcagcggc gggatccagg acacctgccc ggcctgggtc
     1381 cggctgaaca ggatcaacgg cagggcaccc accgtggcgg ccgccgcggc cgtggaccag
     1441 cgtgcccaca cccgccgttc ggccctcacc cacatcaggg tcgtcccgtg cgcgggcagc
     1501 agcagcaacg acagccagtt caagagcgcg cacacgagga cgatccctcc gtacgcgacc
     1561 cagtgcgccg ttcggccgtg gccccggagc acgcgcacca gcagcagggt cgagagcgcg
     1621 gccccgacca tgaccagggc gtacgggcgt ccttcctgga ggtagaactg caccgccggc
     1681 agcagcccga gcgccagccc cgcggtcagg ccgacccggg ccccggcgag gcgccggccg
     1741 atgacggtcg tgcaggccgc ggccaccgcg acggccagga cggagggcag tctcaggacg
     1801 acgacgcccg ggccgaagca ctcgaagagc gcgtgcatga acaggtagta cagcccgtgc
     1861 acggcgtcca cctggccgag catgtgccag atctccccgg cggaccggcg agccacctgc
     1921 caggtcgccg cctcgtctcg ccacacgctg tgccggcggg tcagtcccca caggccgagg
     1981 gccgaggtcc acagcagggg aacgacccac aggggcaccc gacgccggag aacggagggc
     2041 cggagaacgg acagccggag aacggctgtg cggcgcgtca tgaccgggct gccccgacgg
     2101 tgaacacggc gtcgcaccgg tccttgacgc ggtcgtcatg ggtggcgacg acgacggtgc
     2161 agccggcctc gctcatctcg cgcaggaggt cgacgaccgc ggtggcgttg gcatggtcga
     2221 gggcgccggt cggctcgtcg gcgagcacga gggccggctc cttgacgatg agccgggcga
     2281 gggccacccg ctgctgttcc ccaccgctga ggtggtggac cttctcgtcc cctcgtcccg
     2341 cgagcccgac ccggtcgagg gcctcggcga tgtcgctgtt cgccctgccc cgcgcggacc
     2401 gtctcggcct ggtcgcgatc tccaggttct ccctgacggt ggcgttctcc atcagggcgt
     2461 agttctgaaa cagatagccg agggtctcgc gtcggaaacg acgggtgtcg cgccgcccga
     2521 accgggtgat gtcctccccc tcgaaacgga tttcaccgga attcggcttg tcgaggaggc
     2581 cgatgcagtt gaggagggtg gatttgccgg ccccgctggg gccgacgagg gcgagcatct
     2641 cgcccttgcc gaccgtggtg ctgaggccgg accacagagt ccgggggccg aaggtcttcg
     2701 acaggctgac gatatcgatc acgacggggc tcctgctgga tggagagaat tcatacgggc
     2761 gcgtcctgcg ccggcatcag gcttcggcgg cccgctcttt gacggtgcgc cggtgaaata
     2821 caccgagagc aagaagaacg gcgccgaatt cgacgacgac gagccccgcg atgacgccca
     2881 gatccagtcc ggtgagatgg acggccggga aaggggcggg aatgccccgg gccgcgagtt
     2941 cctcgaggtc ccgattctgc tgccaggtct ggatcggaac ccacccggcg aggaaggccg
     3001 ccagaacacc ctcgacgccg agaatgaatc gatgggttgc cgtgaaacgc cagccgttga
     3061 ggtactgggc gaacgccgcc tgggcgttct tgcgcgagtg gatcacgcag actccgatgc
     3121 cggtgatgag gagtacggcg agggaggcga gcagattgaa gaacagcagc cggaagtcgt
     3181 tgaccgccga acgcagactc tcggcggcgt tctgtccgac ggggttcacc gtggccacat
     3241 aggtctgaag gtcgtggtcg ttggcgtgga gaggaccgag gacgtcgccg gggtcgggga
     3301 agacgattgc gtcctgggtc gcgaacgtgg tgtacgcgtc gtccgtgagg atcctcgaac
     3361 cgttggggac cacgacgagc accggatcgc gcaccagggt ccggtcgtcg tccggcgtgt
     3421 aggcggcgtt ggggctccgg tccccggagt tgtagccgaa gacgcgctga ccgctcttcg
     3481 ccggaagggt ctccaccgtg aggcgcccgc gcacctcggg gtcgagcctg ccgaacacgt
     3541 cggggacggc cgccctgatg gccgacgcgt ggtgagcgag cgactgagga acgaggagcc
     3601 gcaccgtgtg tgcgtccgcc gctccctcgc cgtcgtgcgg gagcggggcg tagggccgcc
     3661 cctccgggtc gacgacgcgc tgctcggcga ggaaggactc gttgacgacg agcatctcgc
     3721 cctggggaag gcgggcgttc gagggaaggt tccgcaggtc gtggcggccc gcgacgacga
     3781 tctcgccggc cgcgtccgcc ttccgcagcc aacggccgac ggcggtcacc acgtgcgccg
     3841 agtcgcctcc ccaactgccg ttgaggcgga tggtgacggc gtcgccggcc ttggcgtaga
     3901 cgtcctggtt gttctggcgt gccgccacgt gctggcccgc cgagacgacg tccatggcga
     3961 agccgagcgc gagcagtacg gcgggtacgc gcaccagata ggtgacggcg aaggccgttc
     4021 gggcgggcag ctcgcccttg agcgcgcgga gcacgtcgac cttgaaggtg agggcgagcg
     4081 cggcggcgtg ggcggccacc accaacagca cgagcagacc ggcgaggcag acggccaccg
     4141 aggcgaagag gccgaggtgg gcgagcccgt tgtagaggcc gagacaggtc agggcggtgg
     4201 ccgtcacagc ggcggcggcc gccgcccaga agaccgccaa ctgcctgagg tcgcgcagga
     4261 gaaggtccgc cagggatatg ccttgcagac gcagcaccgc gtaggtcttc gcgctgagca
     4321 gcacgctcgc gccggtcatc gtcaccgcgg cgagtgcgac cacgccgagg gaccagaaca
     4381 gctcgccctc ggtgaagccc cgggccagcg cgccgggtgc caggggatgc tgcacggttg
     4441 cgctcaggcc gagatcggcg aagcgggcga cgaggtcgtc cgcggcgccc ggcggcccga
     4501 agacgtagta gaaaccccgc ggatcgcgct gcccgatctc ggcgagggga tgcacagcgg
     4561 tgtcgacgtg cgaaccgaag gccggatagc cgtccgtcag ccacgcctcc gcgtcggagg
     4621 ccgagtcgcc cgaggccagg tacaggtgac ggagccggtc gggcctcttc acgtcgggca
     4681 gcgcgcgagc gaccgcgacc tcgtggtgat ccgcgaactc cgcgatcgca ccggcgatct
     4741 gcccgccact gacggagtcg tcggacccgg tgacccagac ctgggccgaa ctgcccagaa
     4801 ccaactcctc gtccagtcca cgcacgaaca gaaaggacag gatcgccgag aagagaagaa
     4861 cggcgacatg gacgaacttg atacctcggt ggagcatcgc gttacctaga ttcgggcact
     4921 cggaagcgcg tttctcgtgg ggggagacgt gaggggggac ccggctgcgg agcgcgaaag
     4981 accccgcagc cgggcgcgga tcagcaggcc gggtcgtagt acgccttgtt gagcgcccac
     5041 gtcgacttct tggccgacgc cttcgaccag accttttcct tggtgcagcc gctcttggcc
     5101 cagtacgcgc cttccacgga ggacgagtgg tagttgtcct tgtggtagta gttcgagtac
     5161 acgtaattgg cgcccacacc gtggctccac ttgccgccgc cggcatcctc ggcggccagc
     5221 gcgggcgcgg cgaaggccat gaccacgaca ccggttgcgg caacggcctt gatgctgttc
     5281 ttcaggctca acatgatttc tctttccttc ctgaagcccc ggtttttggc atgcgtcgac
     5341 cgaggctcgt tttcgatggt tgtttttgct gctaaatcag tgatctacgg gtctacggat
     5401 gcacgaatgc accgatctac gaatgcaccg atctacggat gcacggatct acggatcagg
     5461 ccgcgatcca tggaacacgg caggcgtgcg gctgccggcc ctccgggcac agacctctga
     5521 tcacggactt gcgaatggcc cgccacgtct tcagcgagca cttcagccgg acgcacagtt
     5581 cgactgtttc ccgcgtcccg ctgctctggc cggggtgctc caagcgccac tgctcggaca
     5641 ggtggtcgta cacatcggcg gtgggtagtt cgccaccgag cacggtgtgg gcgacggttt
     5701 ccccgacgat ccgagcactg tgggagccga cagactcctc gacaagcgct ctgacccggg
     5761 tcagaccccg ttcgtccgcg tacagcccga acctgagcgt gcgcgtccgc aaggcggtca
     5821 gcccctgacg atcttcttct tgacgcagtg cttcgcgcgg tagccgcggg gcatctggtc
     5881 gttggcgtcc ttgatggccg ccttccaggc gaggtccttg gtcttggccc acccgtggcc
     5941 gttgaagaag ccgtggccgg agctgtcctc gatgcggaac ttggtgcact gcacggtcgc
     6001 gttccagccg ttggcggcct gcgcgggcgc ggcgacaccg acggagagcg tggctgccac
     6061 gacggcggtg agcagtgggg aaagggcaat caatttgcgc atgactgcgt ctccaggggg
     6121 ttggtctctc atccggcagg gggcaggtcg gcgttgtggc ccacagatgc cccggcacag
     6181 ccgggcatgc cggacaacgc ctcgactgca ccagccgaga cgggatccga gcgttggtcc
     6241 ccctaccccc tgagcgcacc cgccgttggt cagacgagcg agtggccggt agagacatta
     6301 cccaggccga tgatcatgag cgaccgatca cgcctatgaa caagatcacg acaagcgcag
     6361 tgaacctcga gccctcacgg gtgccggatt ggcccccttc aggagagatc ggcaggttgg
     6421 ggcgcattgg taccgtcggt gcaagcccag cttcgagaag gaccccggac cgcgactggt
     6481 ccgcgtcttc ggtgacccgc cgggtcatac ccaggtctga ccccgcggtt cagctcctgg
     6541 tccgacgccc cgcgggggac ggcgaaggca tcgtcgtcgc atgacgaacc cgacgacgcc
     6601 cacggccgcg ccggtcacga cgatcggcgc ccgactgcga gtcgcgctgc ctgtggaccg
     6661 gtgggaggcc accggcttcc ccggacgttg ggtgggcggc accgcgatga cgctcggccc
     6721 actcctgatg ctgaccggcg cgttgctgcg ggtccgcttc cccttcttct tccccgacca
     6781 actcgccgcg tacgaacggc accccacgct gatggccacc gcgtacggcc tcttcgcggc
     6841 gggcggcctg ctgctctggc ccgccgtggc ggtgctggcc gcacggatcg gggtgcggag
     6901 tgccggctgg ggcctgtggg gcggggtcct ggtgatgttc gggctgttcg cccgcgcctt
     6961 ccacgcgggg gtggaccatc tggcctttca gctggtgcgt tcccaggggg ccggccccgc
     7021 caccgaagcg gtctccgccg gctacggtgc cttccacgtc ttcgccacgc tgaacctcgc
     7081 catcctcgcc gggtggatcg tcctcgccct cggcgcctgg cgcacccggg tgctcggtcc
     7141 ggcccgcgcc gccgcactcg gtctcacctc ggcgctgccc ctcggcgtac tcaaggggac
     7201 gaccccgctg tcgctggtgg ccctggccgg actgtgcgcc gcactggtgc cgctgggcct
     7261 ggccctgctg cgtgaagcgc cccgccccag ccgtgcggcg gtgctgcgct gggtaccgct
     7321 gaccctggtg acgctcgggc tgatggtgct gctgggacag gccggttgag tgcacacgtc
     7381 ccagtaccgt cgaaccatga agcccaggcg gcagcgccat ctccatgccc tcgacctcgg
     7441 cgcggctctg gccctgaccg ccgtgtacat cggcttcgcc cggctgaccg ccgaggacgg
     7501 ccagcccgtc tacacaggac ccttctggct gggctgcctg atcgcggccc tggtcggcct
     7561 gccggtcgcc gtacgccgcc gatggccgct cgcggtcctg gcggccgtac tgaccgctct
     7621 ggccgtggcc tcgctcctcg acatcccccg tgagccgtac accgccgccg ggctcgcggc
     7681 ctatctcgtc gggctggccg agcccgcccg ccggtccgtg cccgcgctcg cggtgacact
     7741 gccggtggcg tgcggtgccg tctacgtcgg ggaagcggtg gtcactcccg ccgaggactg
     7801 gcggggcgcc gcaggggtgg ccgggctcgt gctcctggtg atcggcgggg cctggagcac
     7861 ggggttcacc gtgcgcggcc gccgggccga ggccgccggg gagcagcggc gccgctcgga
     7921 gcgcgcactg gacgaggaac gtctgcggat cgcccgcgag ctgcacgaca tcgtctcgca
     7981 caagctcagc ctgatcgccg tcaaggcggg cgtcgccggg cacgtggcgg aggccgaccc
     8041 gcgggaggca cgggctgccc tcaaggtcat cgaggagacg agccgttccg cgctggccga
     8101 aatgcggcgc acgctcggtg tgctgcgcac cgagggcgcg cccctgggac ccgtaccggg
     8161 gctcgaccgg ctcgacctgc tcgcggagga ggcacaccgg gcgggggtgg aggtcgatct
     8221 gtcggtgcac gccggggacg gcctggccga ggggacacag gtgaccatct accggatcgt
     8281 ccaggaggcc gtcaccaacg tggtccggca cgcggccccg acccgctgcc gggtgacgat
     8341 cgaggtcgac gcgccagagg tccgcatcga cgtcgtggac gaggggccac cgcccgcaca
     8401 ccgaccggcg gttaccggac tcccgggcgg acacggcctg ttggggatgc gggagcgcgc
     8461 gatgatgtac ggcggcagct tcgaggcggg cccccggccg gagggtggct tcgcggtgtc
     8521 cgtccgcctg ccgaccgagg ggaaccgatg accacgaccg acgcgactcc cgtccgggta
     8581 ctgatcgcgg acgaccaggc gatggtgcgc ggcagcttcc gggtcctcgt cgatcacact
     8641 cccggtatga cggccgtcgg cgaggccgcg aacggagcgg aagccgtcga gctcgcccgc
     8701 cgcgaacggc cggacgtcgt actgatggac atccgcatgc cggacctcga cggcatcgag
     8761 gcgacccgcc agatctgcac ggacccggcc ctgcccgccg tccgtgtcct catcctcacc
     8821 accttcgacc tggacgagta cgtctacgcc gccctgcgcg ccggcgccgc gggcttcctg
     8881 ctcaaggaca ccccgccgtc cgaggtcctc accgccatcg gtgtcgtcgc ctccggcgag
     8941 gcgctgctcg ccccctcggt cacccgccgc ctgatcgcgg agttcgcccg ccgtccggag
     9001 cctgcgcggc cgcccagccg ctccctggac gggatcaccg accgggagct ggaggtgctc
     9061 ggcctgatcg cgcggggcct gtccaacacg gaactcgcag aacacctcca cctgagcctg
     9121 gccacggtca agacccatgt cggacgcctg ctcaccaagc tgcgtgcgcg cgaccgcgcc
     9181 cagctcgtca tcgtcgccta cgagaccggg ctggtcggcg accgccgacg tggcgtgtga
     9241 ggagcgcggc gactgtctct agcgctccgg aaggttgagg ccgccgagct tgtaacgcac
     9301 cgagacctca tcggcctgct gctgcacgcg gtcccgcagt tgagccaggg cctcgttgaa
     9361 cctcctgtcg gatccggggc cgaaacgccc gccgttggat ccgcccgctt cgatgaagtc
     9421 cgtgaaggcc tgccgcatgg cacgcagcca gatgcgcagg tcgcttcccg gcttcttcag
     9481 ctgggtcagg tactcggaca gttgagtacg gcactcggcg gccgacgcga ggcaggccgc
     9541 ggcctcggac tgcgggcgcg cgtacccgat cgtcagcacg cgcttgtccg cgaggaagtc
     9601 catgacctcc tgggcgatgg ccctgtccga cttcgtgaac tcccagttca caccaaaccc
     9661 gaaggcactg aacgccgttg gccggatacc catccgtccc ccctccatcg acggtctatc
     9721 cctatcagcc cggaagcgcc gacgcggcgc aaccccgagc ctcaaccgtg gccgccggtc
     9781 ctagctgctg cggcgatcga tccggtgcgg tactggtgca gggctgcggc gcccacgagg
     9841 ctgccgacac gacaaagggc cagcggcgga ggaaaccctc ctgagctgtc cctctgctct
     9901 gtgcccccgg caggatccga acctgtgaca cccgccttta ggagttcgat ccgagcccgc
     9961 tccggcagcc gtcgtcggct ggtgcgacag caggacaggg atgccggtgt acgcccccgt
    10021 ctggcgtcgt tgatgtcagc cttggatgtc agaaaccccc tgatctgtag ctttccgtcc
    10081 ggcgcagcga cactgcctgc cgcggttgcc gctcaagact cgcgtctgtt cgcctcgcag
    10141 cgcttgaata ggtagtagcc cacggtttcc cccttgatcg cgacgttctg gccggacgac
    10201 atgaacttgt cgcggctctg ctgtccttgc tctacgaaga cgaatgagcc gttcaccagt
    10261 tcccacccct cccggcacac ggcgttgagg acga
//
