LOCUS       NZ_CP085300            13012 bp    DNA     linear   CON 18-JAN-2024
DEFINITION  Streptomyces sp. BSE6.1 chromosome, complete genome.
ACCESSION   NZ_CP085300
VERSION     NZ_CP085300.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. BSE6.1
  ORGANISM  Streptomyces sp. BSE6.1
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8024389)
  AUTHORS   Ramesh,C., Anwesh,M., Vinithkumar,N.V., Kirubagaran,R. and
            Dufosse,L.
  TITLE     Complete Genome Analysis of Undecylprodigiosin Pigment
            Biosynthesizing Marine Streptomyces Species Displaying Potential
            Bioactive Applications
  JOURNAL   Microorganisms 9 (11), 2249 (2021)
   PUBMED   34835376
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8024389)
  AUTHORS   Chatragadda,R. and Maile,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-OCT-2021) Andaman and Nicobar Centre for Ocean Science
            and Technology, National Institute of Ocean Technology, Dollygunj,
            Port Blair, Andaman and Nicobar Islands 744103, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 10-OCT-2021
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/18/2024 07:10:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,273
            CDSs (total)                      :: 7,198
            Genes (coding)                    :: 7,094
            CDSs (with protein)               :: 7,094
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 47 of 104
            Pseudo Genes (incomplete)         :: 80 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 30 of 104
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 01:03:07
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1556716
            Orig. end                         :: 1569728
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP085300.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from National Institute of Ocean
            Technology.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..13012
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="hydrogen-cyanide"
                     /region_number="8"
                     /rules="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD) and DAO)"
                     /tool="antismash"
     cand_cluster    1..13012
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /kind="single"
                     /product="hydrogen-cyanide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..13012
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:8012](-)"
                     /cutoff="5000"
                     /detection_rule="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /neighbourhood="5000"
                     /product="hydrogen-cyanide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..8012)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="5000"
                     /detection_rule="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /neighbourhood="5000"
                     /product="hydrogen-cyanide"
                     /protocluster_number="1"
     gene            complement(395..2269)
                     /locus_tag="LJA35_RS06835"
     CDS             complement(395..2269)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005309653.1"
                     /locus_tag="LJA35_RS06835"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_212731685.1"
                     /transl_table=11
                     /translation="MGTTDTRGPAPGRSAVVLALRHYGRELLRQRRLALPALLLPAVGN
                     IGIRYVAPLLIAKLAGQAADGGGLALGPALPYVLGFGVTLLLAEGIWRIGQHCLNRVDA
                     LGMEHLYVSGMDELLAKDAAFFHDNFAGSLTKRVLSYGKRFEDFVDTLTYRIVGSLVPL
                     VFGAVVLWSYEPMLVVGLFAMIALTVAAATPLIRRRQRLVNDREAAIARVSGHVADSLM
                     NMETVRAFAAERREGAEHRSRVAESRRLTLKSWDYGNLRVDTLIAPLSVLTNVLGLVVA
                     LALGGPGQGVEEVVVAFTYYSNATQIMFEFNQIYRRLESSMTEAAQFTELLLDPPTVLD
                     ADEPEPLAPEDTGVRFETVTFAHTGAEPIFRGLDLDVPAGARIGLVGRSGGGKTTLTRL
                     LLRMSDIDHGRILIGGQDISRLRQTDLRSLIAYVPQEPAMFHRSLRDNIAFARPGATDE
                     EIHAAAAAAHVTEFAHQLPDGFATLVGERGVKLSGGQRQRVALARAILRDAPILLLDEA
                     TSALDSESEILVQDALWRLMDGRTALVVAHRLSTVAGMDRLVVLDRGRVVEQGSHEKLL
                     ATDGAYARLWQHQSGGFLGESADSPAGHPAPEAGPGEPPGPADPVPVA"
     misc_feature    complement(2258..2273)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     misc_feature    complement(2382..2398)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 16.35"
                     /tool="antismash"
     misc_feature    complement(2384..2394)
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 20.42"
                     /tool="antismash"
     gene            2434..3114
                     /locus_tag="LJA35_RS06840"
     CDS             2434..3114
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1071: GntR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018565783.1"
                     /locus_tag="LJA35_RS06840"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="GntR family transcriptional regulator"
                     /protein_id="WP_102929827.1"
                     /transl_table=11
                     /translation="MPHLKSRAISVQTHLRDQVADALRAALIAGDLRPGVVYSAPSLAA
                     ELGVSATPVREAMLDLAREGLVEAVRNKGFRITEMTERDLDEFTELRTLIEVPTVGRVA
                     ATATAEQLEALRPLALRIVTAAREHDVLEYLEADHCFHLALLGLAGNRHLVEVVADLRK
                     RSRLFGLGSLNEAGRLVASAQEHVDLLDLMVAGRGAEAEECMRRHLAHVRTLWADGTAP
                     SVRG"
     gene            complement(3104..4105)
                     /locus_tag="LJA35_RS06845"
     CDS             complement(3104..4105)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019891275.1"
                     /locus_tag="LJA35_RS06845"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="proline racemase family protein"
                     /protein_id="WP_102929828.1"
                     /transl_table=11
                     /translation="MRSTVCYHAVDSHTEGMPTRVITGGVGVLPGATMFERRQRFVAER
                     DDLRTLLMCEPRGHASMSGAILQPPTRPDADYGVLFIEVSGCLPMCGHGTIGVATVLVE
                     TGMVEVAEPETPVRLDTPAGLVTARVRVRDGHAESVTLENVASYCHALDQVVDVPGHGR
                     VRYDIAYGGNFYAFVRTEDLGIPFERAHKQRLLDAGLAVMDAINKQNPVSHPENPEIDV
                     CHHVYLEAPGSTAEHSRHAMAIHPGWFDRSPCGTGTSARMAQLHARGLLPAGRDFVNES
                     FIGSRFVGRILGETTVGDRPAVLPSVTGRAWITGTAQYLLDPADPYPAGFTL"
     gene            complement(4108..5004)
                     /locus_tag="LJA35_RS06850"
     CDS             complement(4108..5004)
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /GO_process="GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /GO_process="GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011030799.1"
                     /locus_tag="LJA35_RS06850"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="WP_102929829.1"
                     /transl_table=11
                     /translation="MSETRKPWHGVIVATSLPFHDDLSVDFGAYGESVAHLAEQGMHGV
                     APNGSLGEYQTLTYEERDRVVETAVANAPAGFTVMPGVGAYGGGEARRHAQFAKDAGCQ
                     AVMCLPPNTYRADDRAVLEHFETVASVGLPVTAYNNPIDTKVDLRPDLLAKLHAEGFIV
                     GVKEFSGDVRRCYEISELAPGLDLMIGTDDTVLEVALAGAKGWVAGYPQVFPRACLALY
                     EASIAGDLETALPLYRRLHPVLRWDSRTEFVQAVKLGQELTGRRGGVCRPPRQPLAPET
                     EAVVRAATQALIDAGVN"
     gene            complement(5001..6569)
                     /locus_tag="LJA35_RS06855"
     CDS             complement(5001..6569)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Fer2_BFD"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Pyr_redox_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1176: alkyl hydroperoxide reductase subunit"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011030798.1"
                     /locus_tag="LJA35_RS06855"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="WP_233645288.1"
                     /sec_met_domain="Pyr_redox_2 (E-value: 2.5e-18, bitscore:
                     65.0, seeds: 64, tool: rule-based-clusters)"
                     /sec_met_domain="Fer2_BFD (E-value: 1.3e-10, bitscore:
                     40.3, seeds: 211, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSEPADLVVVGAGPAGMAATATALTGGLRVVLVDSGTALGGQYWR
                     HPPEHARAAVPTDDLHHGLRAYRALCRALTAHRAAGRLDLRLEHHAWSAVREGDGFAVH
                     VVDRRAAPRETAGVVRAPRLLVATGAYDRQLPFPGWDLPGVLTAGGLQALLKGGGVAAG
                     SRVALGGTGPFLLPVAAGLAARGVEVVAVCEAAHPRAWLRRPGPLLRNPAKWAEGAGYA
                     AALARHRVPVRPRTAIVAAEGRERVTSVRIASLTPDGSPRPGTERRVEVDAVGVGWGFT
                     PQLDLLVPLGCALTDSADGNAVAVVDAGQRTTVPGLYTAGETCGVGGAALAVNEGRLAA
                     QSVLTDSGAPHVDANRSLAAVRSAVDRQRAFARAMALAHPVPPAWSSWLTDETVVCRCE
                     EVTAAAVRTAQAAGAQDHRQVKQLTRAGMGWCQGRMCQPAVHCLTARERAYEPAERLVA
                     TPVTLGALADLDEGPPPQTPTTPQAHTTPQAPATPQAPTTPQAPEAPPAPSGPAANGAT
                     DKECS"
     gene            complement(6562..6816)
                     /locus_tag="LJA35_RS06860"
     CDS             complement(6562..6816)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Fer2_4"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003972635.1"
                     /locus_tag="LJA35_RS06860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="WP_102931151.1"
                     /sec_met_domain="Fer2_4 (E-value: 2.2e-23, bitscore: 80.8,
                     seeds: 114, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSEIVVDGDPLPFTEGQTVAAALVSAGRVAWRTTRVGRRPRGVFC
                     GIGVCFDCLVTVDGTGGQRACLVPARPGMTVTTREDDDE"
     gene            complement(6813..8012)
                     /locus_tag="LJA35_RS06865"
     CDS             complement(6813..8012)
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /GO_function="GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1103: FAD dependent oxidoreductase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003972636.1"
                     /locus_tag="LJA35_RS06865"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="WP_233645289.1"
                     /sec_met_domain="DAO (E-value: 9.1e-66, bitscore: 221.5,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTLKIVVVGAGVVGAACAFHAASAGLDVTVVDRGPVGAGTTSRGE
                     GNVLLSDKEPGPELALAQLSRTLWDEAGRELGPDSLELEAKGGLVVASTAEGLAALGDF
                     AARQEAAGVRVEPVDRVRDLEPHLAPGIHGGVHYPQDAQVQPVLAAAALLRAAVRHGAR
                     FHTGEVASAVTARDGGRTPGDRSTGGRITGVRTTVGDLLPADAVVNAAGTWGGEVGRRL
                     GAPVEVLPRRGFVLVTEPLPPMVRHKVYSADYVANVASSDAGLETSCVVEGTRGGTILI
                     GASRERVGFDTTMNPAVVARLAAQACRLFPFLRGVHLMRAYRGFRPYCPDHLPVVGPDP
                     RVPGVVHACGHEGAGIGLAPATGALVTAHLLGRPWRGADPAAHTALLPDRFLNPGGAPQ
                     "
     misc_feature    8068..8078
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: strong, score: 22.4"
                     /tool="antismash"
     gene            complement(8141..10252)
                     /locus_tag="LJA35_RS06870"
     CDS             complement(8141..10252)
                     /GO_function="GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020133419.1"
                     /locus_tag="LJA35_RS06870"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="SWIM zinc finger family protein"
                     /protein_id="WP_233648232.1"
                     /transl_table=11
                     /translation="MAREALRAARRQAKAAAASGGAAHARRAPRPAGRQRRTGGTGTAD
                     ARARAVRDFVADAFRLPSEADPPEETSPAGQTGESQPADKHAGRTSHEVRAALPSPEAT
                     SSSGVGSAVGSGSVGSGEVGAAASSSAAASSFAVGPATGSGPAGAREEGAALSSPEAVS
                     SSGVGPVTGPGPAGSGEDRVAALSPEPAPSSGPALSPDPAPSPESTPPPGVGPVIGAGS
                     EPAAVSPRAGHAPRSEGSASDSERPEGPRRAEQPSAGRDRDADAAHSAPPARPRAPRSM
                     GAPGRDGELRRTFPALPPRNAADEGLAGTWWGNAWVTALEEGALDPARLERGRGYAERG
                     RVDAITVTPGLVLAYVQGSRARPYRVQVRLRTLGDAHWDRFLDAAVERPGHIAALLDKE
                     LPHSLADLADRGVPLLPGPGDLTPQCSCPDSGHPCKHAAALCYQTARLLDADPFVLLLL
                     RGRGERALLDALSRRNAARAARAAQERGPAPLPGVRAGEVLARRVLPPLPAPLPAPPHP
                     EQPPAYPAAPGGPDPFALDQLATDAAARAHALLTTGRDPVGGLTLWQDAVRLAAARPGS
                     GLTAGTRALYASLASAAGRDTAELARAVAAWRQGGPESLDVLEEPWDPPAGRFDRARPL
                     LLAADLPAFRPWRNRLTHPRGHVQLRLGRAGLWYAYESEPGREDWWPRGTPDLDPVGAL
                     TGLGTRVDL"
     misc_feature    10767..10781
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: strong, score: 20.47"
                     /tool="antismash"
     misc_feature    complement(11956..11972)
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 18.89"
                     /tool="antismash"
     misc_feature    complement(12080..12093)
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 18.58"
                     /tool="antismash"
ORIGIN
        1 tcccactcca gggtgccgtc ctgctccttg ccgtcggggc cgtcggtggg cacctcgaag
       61 gcgtgcacgt cgaccgcgtc gaccgtgcac gcctccaacg gcgtggtgcc cccctcgtcc
      121 ggcgctgtcg agccgctgcg catcagtggc gtccggggag ttcgtcgagg ccgtcacccg
      181 gacagccgta cgcccgctcg acgccccgcc cggtcgggcg ctggaggacg gagtcggcga
      241 cttgggtcat gcggctctcc tgggcaggcg ggaagaacac tcatggagtg ccttccctga
      301 acgccggtaa accggacgac ggtcactcgt tggggcgagt cgggcaccgc gccggccgcg
      361 ccgcagccgc cggccggcgc ggcgggcggc acgctcacgc caccggtacc gggtcggccg
      421 gtccgggcgg ctcgccgggg ccggcctccg gggcggggtg cccggcgggc gagtcggcgc
      481 tctcgccgag gaagccgccc gactggtgct gccacagccg ggcgtaggca ccgtccgtcg
      541 cgagcagttt ctcgtggctg ccctgctcga cgacgcgtcc gcggtcgagg acgacgaggc
      601 ggtccatgcc ggcgacggtg ctcagccggt gggcgaccac gagggccgtg cgtccgtcca
      661 tcagccgcca cagggcgtcc tggacgagga tctcgctctc cgagtccagc gcgctggtcg
      721 cctcgtcgag caggaggatc ggggcgtcgc gcaggatggc ccgggcgaga gcgacgcgct
      781 ggcgctggcc gcccgacagt ttcacgcccc gctcccccac cagggtggcg aagccgtcgg
      841 gcagctggtg ggcgaactcg gtgacgtggg cggccgcggc ggccgcgtgg atctcctcgt
      901 cggtggcgcc gggccgggcg aaggcgatgt tgtcccgcag gctgcggtgg aacatggcgg
      961 gttcctgcgg cacgtaggcg atcagtgagc gcaggtcggt ctggcgcagc cggctgatgt
     1021 cctggccgcc gatcaggatg cggccgtggt cgatgtccga catccgcagc aggagccggg
     1081 tgagcgtggt cttgccgccg ccggacctgc cgacgaggcc gatccgcgcg cccgcgggca
     1141 cgtccaggtc gaggccccgg aagatcggct cggcgcccgt gtgggcgaag gtgaccgtct
     1201 cgaagcggac gccggtgtcc tcgggcgcga gcggttcggg ctcgtcggcg tccagcacgg
     1261 tgggcgggtc gaggagcagc tccgtgaact gcgcggcctc ggtcatcgag ctctccagcc
     1321 gccggtagat ctggttgaac tcgaacatga tctgggtggc gttggagtag taggtgaagg
     1381 cgacgacgac ctcctccacg ccctggcccg ggccgccgag ggcgagggcg accaccaggc
     1441 ccagcacgtt ggtcaggacg gacagggggg cgatcagggt gtcgacgcgc aggttgccgt
     1501 agtcccacga cttcagcgtg aggcgccggg actccgcgac ccggctgcgg tgttcggccc
     1561 cctcccggcg ctcggccgcg aaggcgcgga cggtctccat gttcatgagg ctgtcggcga
     1621 catggccgga gacccgggcg atcgccgctt cccggtcgtt gacgagccgt tgcctgcgcc
     1681 ggatcagcgg ggtcgcggcc gccacggtca gcgcgatcat cgcgaacagg ccgacgacca
     1741 gcatcggttc gtagctccac agcaccacgg ccccgaacac cagcgggacg aggctgccca
     1801 cgatgcggta cgtcagtgtg tcgacgaagt cctcgaagcg cttgccgtag ctgagcaccc
     1861 gtttggtcag ggagccggcg aagttgtcgt ggaagaacgc ggcgtccttg gcgaggagtt
     1921 cgtccatgcc gctcacgtag aggtgctcca tgccgagcgc gtccacgcgg ttcaggcagt
     1981 gctgcccgat ccgccagatg ccctcggcga gcagcagcgt cacgccgaag cccagcacgt
     2041 acggcagtgc cgggccgagg gcgagtccgc cgccgtcggc cgcctgcccg gccaacttgg
     2101 cgatcagcag cggcgcgacg tagcggatgc cgatgttgcc gacggccggc agcagcagcg
     2161 cgggcagggc gagtcgtcgt tgtcgcagca gttcccggcc gtagtggcga agcgccagga
     2221 cgaccgcgct cctgcccggt gccggccctc gcgtatctgt ggtccccatc acactcccgg
     2281 tggttcggaa gtcggaagtc tcccgtcgga ccaggtacgc agtccaggca tttaccgcgg
     2341 accgtcgaga accggacatc gtgcggcccg tcgtgggcgc aacgtgacat tgtatggtga
     2401 ggcgccattg gccggccgga cgcaggagca cggatgccgc atctcaagtc ccgggcgatc
     2461 tccgtccaga cccatctgcg cgaccaggtc gccgacgcgc tccgggccgc cctcatcgcc
     2521 ggtgatctgc gtcccggtgt cgtctactcc gccccttccc tggccgccga actcggcgtc
     2581 tccgccactc cggtgcgcga ggccatgctg gacctggcca gggaggggct ggtcgaggcg
     2641 gtgcgcaaca aggggttccg catcacggag atgaccgagc gggacctgga cgagttcacc
     2701 gagctgcgga ccctgatcga ggttcccacc gtgggccgcg tcgccgcgac cgcgaccgcc
     2761 gagcaactgg aggccctccg gccgctggcg ctgcggatcg tcaccgcggc ccgggagcac
     2821 gacgtcctcg agtacctcga agccgaccac tgcttccacc tcgcgctgct gggcctggcc
     2881 ggcaaccggc atctcgtgga ggtggtcgcc gacctgcgca agcgttcccg cctgttcggg
     2941 ctggggagcc tcaacgaggc ggggcggctg gtcgcttcgg cccaggagca cgtcgacctg
     3001 ctggatctga tggtggccgg ccgcggtgcc gaggccgagg agtgcatgcg ccgccatctc
     3061 gcgcacgtcc gcacgctgtg ggcggacggc accgccccgt ccgtcagagg gtgaacccgg
     3121 ccgggtaggg gtccgcgggg tcgagcaggt actgcgcggt gccggtgatc caggcgcggc
     3181 cggtgacgga cgggaggacg gccgggcggt cgccgacggt cgtctcgccg aggatccggc
     3241 cgacgaaccg ggagccgatg aacgactcgt tgacgaagtc ccggccggcc ggcagcaggc
     3301 cgcgggcgtg cagctgcgcc atccgggcgc tggtgcccgt gccgcagggc gatctgtcga
     3361 accatcccgg gtggatcgcc atggcatgcc gtgagtgctc cgcggtggat ccgggggcct
     3421 ccaggtagac gtggtggcac acgtcgatct ccggattctc cgggtgggac accgggttct
     3481 gcttgttgat cgcgtccatg acggccagtc ccgcgtcgag cagccgctgc ttgtgcgccc
     3541 gttcgaaggg gatgccgagg tcctcggtgc ggacgaaggc gtagaagttg ccgccgtagg
     3601 cgatgtcgta gcgcacccgg ccgtggccgg ggacgtcgac gacctggtcg agtgcgtggc
     3661 agtacgacgc cacgttctcc agggtgaccg actcggcgtg gccgtcgcgc acccgcaccc
     3721 gggccgtgac gagtccggcc ggtgtgtcga gccggaccgg ggtctccggt tcggccacct
     3781 ccaccatgcc ggtctcgacg aggaccgtgg cgaccccgat ggtgccgtgc ccgcacatcg
     3841 gcagacagcc ggagacctcg atgaagagca cgccgtagtc ggcgtccgga cgggtgggcg
     3901 gctgcaggat cgccccggac atcgaggcgt gcccgcgtgg ttcgcacatg agcagggtgc
     3961 gcaggtcgtc gcgttccgcg acgaaccgct gccgtcgttc gaacatcgtc gcgccgggga
     4021 ggacgcccac cccgccggtg atcacgcggg tgggcatgcc ctcggtgtgc gagtcgacgg
     4081 cgtggtagca gaccgtcgag cgcatcgtca gttcaccccc gcgtcgatca gggcctgggt
     4141 ggcggcgcgg acgacggcct ctgtctccgg ggccagcggc tgccgcggcg ggcggcacac
     4201 accaccgcgg cggccggtca gctcctggcc gagcttgacg gcctggacga actccgtcct
     4261 gctgtcccac cgcagcaccg ggtgcagccg gcggtagagc ggcagtgccg tctccaggtc
     4321 gcccgcgatc gacgcctcgt acagggcgag gcaggcccgg gggaagacct gtgggtaccc
     4381 ggcgacccag cccttggcgc cggccagggc gacctccagc acggtgtcgt cggtgccgat
     4441 catgaggtcg aggccggggg ccagttcgga gatctcgtag cagcgccgga cgtcgccgga
     4501 gaactccttg acgcccacga tgaacccttc ggcgtgcagc ttggccagca ggtcggggcg
     4561 caggtccacc ttggtgtcga tggggttgtt gtacgcggtg acgggcagtc ccaccgaggc
     4621 caccgtctcg aagtgttcca gcacggcgcg gtcgtcggcg cggtaggtgt tgggcggcag
     4681 gcacatcacc gcctggcagc ccgcgtcctt ggcgaactgt gcgtgccgcc tggcctcccc
     4741 gccgccgtag gcgccgacgc ccggcatgac ggtgaatccg gcaggcgcgt tcgcgacggc
     4801 tgtctcgacg acgcggtcgc gctcctcgta cgtgagggtc tggtactcgc cgagcgatcc
     4861 gttgggcgcg acgccgtgca tcccctgctc ggcgaggtgg gcgacgctct cgccgtaggc
     4921 accgaagtcg accgagaggt cgtcgtggaa cggcaggctg gtcgcgacga tgacgccgtg
     4981 ccagggcttg cgggtctcgc tcatgaacac tccttgtcgg ttgcgccgtt ggccgccggt
     5041 ccggacggtg cgggcggtgc ctcgggggct tggggtgtcg tgggggcttg aggtgtcgcg
     5101 ggggcttgag gtgtcgtgtg ggcttggggt gtcgtggggg tctggggtgg gggtccttcg
     5161 tcgaggtcgg cgagggctcc gagggtgacg ggcgtcgcga cgagccgctc cgcgggctcg
     5221 taggcccgct cccgtgccgt caggcagtgc acggcgggtt ggcacatccg gccctggcac
     5281 cagcccatgc cggcccgggt cagctgcttg acctgccgat ggtcctgggc gccggccgcc
     5341 tgggcggtcc ggaccgctgc ggcggtcacc tcctcgcagc ggcacacgac ggtctcgtcg
     5401 gtgagccacg aggaccatgc gggagggacc ggatgggcca gcgccatcgc ccgggcgaag
     5461 gcgcgttgcc ggtccaccgc cgaacggacc gccgccaggc ttctgttcgc gtctacgtgg
     5521 ggtgcaccgg agtcggtgag gaccgactgc gcggccagtc ggccctcgtt cacggcgagc
     5581 gccgccccgc ccacaccgca ggtctccccc gcggtgtaga gaccggggac ggtggtgcgc
     5641 tgccccgcgt cgaccacggc caccgcgttg ccgtccgccg agtccgtgag ggcgcagccg
     5701 agcgggacga gcaggtcgag ctggggggtg aagccccagc cgacgccgac ggcgtcgacc
     5761 tcgacgcgcc gttcggttcc cgggcggggg ctgccgtccg gcgtgagcga ggcgattctt
     5821 accgacgtca cgcgttcgcg gccttccgcg gcgacgatcg ccgtgcgcgg gcgcaccggg
     5881 acgcggtggc gggcgagcgc ggcggcgtat ccggctccct cggcccactt ggcggggttg
     5941 cgcagcagcg gaccgggacg gcgcagccag gccctcgggt gggccgcctc gcacaccgcg
     6001 acgacctcga cgccgcgcgc ggcgagaccg gcggcgaccg gcagcaggaa cggtccggtg
     6061 ccgcccagcg ccaccctgct cccggccgcc acgccgccgc ccttgagcag cgcctgcagg
     6121 ccgccggcgg tcaggacacc gggcaggtcc cagccgggga agggcagttg acggtcgtag
     6181 gcgccggtgg ccacgaggag cctcggcgcg cgcacgacgc ccgcggtctc ccgcggcgct
     6241 gcccggcggt cgaccacgtg gaccgcgaat ccgtcgccct cgcggacggc ggaccaggcg
     6301 tggtgttcca ggcgcaggtc gagccggccg gccgcccggt gtgccgtcaa cgcccggcac
     6361 agggcacggt atgcgcgcag cccgtggtgc aggtcgtcgg tcggtacggc cgctcgcgcg
     6421 tgctccggcg ggtgccgcca gtactgtccg ccgagggcgg tcccggagtc gaccagtacg
     6481 acgcgcagtc cgccggtcag tgcggtggcg gtcgcggcca tgcccgccgg gcccgcgccc
     6541 acgacgacga ggtccgccgg ctcactcatc gtcgtcctcc cgcgtcgtga cggtcatgcc
     6601 gggccgggcc ggcaccaggc aggcgcgctg cccgccggtg ccgtcgaccg tgacgaggca
     6661 gtcgaagcag acgccgatgc cgcagaagac gccccgtggg cggcgtccga cgcgagtggt
     6721 gcgccaggcg acccggcccg ccgagacgag ggccgcggcg acggtctgtc cctcggtgaa
     6781 cggcagcggg tcgccgtcga cgacgatctc gctcactggg gcgcacctcc ggggttgagg
     6841 aagcggtcgg ggagaagagc ggtgtgcgcg gccgggtcgg cgccgcgcca ggggcggccg
     6901 agcaggtggg ccgtcaccag ggcgccggtc gcgggggcga ggccgatgcc cgcgccctcg
     6961 tgaccgcagg cgtggacgac gcccggcacg cgcgggtcgg ggccgacgac cggcaggtga
     7021 tcggggcagt acggccggaa gccgcggtag gcgcgcatca ggtggactcc gcgcaggaag
     7081 gggaagagcc ggcacgcctg cgcggcgagc cgtgcgacca cggcggggtt catcgtggtg
     7141 tcgaagccga cgcgttccct gctggcgccg atgaggatcg tgccgccgcg cgtgccctcg
     7201 acgacgcacg aggtctccag tccggcgtcg gacgacgcga cattggcgac gtagtccgcg
     7261 gagtagacct tgtgccggac catcggcggc aacggttccg tcaccaggac gaagccgcgg
     7321 cgcggcagca cctcgacggg cgctccgagc cgccgtccga cctcgccgcc ccaggtcccg
     7381 gcggcgttga cgacggcgtc ggcgggcagc aggtcaccga cggtcgtccg gacgccggtg
     7441 atccggccgc cggtggaccg gtccccgggg gttcggccgc cgtcccgggc ggttacggcg
     7501 gacgcgacct cgccggtgtg gaagcgggcg ccgtgccgta cggcggctcg cagcagcgcg
     7561 gccgccgcca gcaccggctg cacctgggcg tcctgcgggt agtggacgcc gccgtggatc
     7621 ccgggcgcga ggtggggttc gaggtcccgc acccggtcga ccggctcgac gcggacaccg
     7681 gccgcctcct gccgggcggc gaaatcgccg agcgcggcga ggccttcggc ggtgctcgcg
     7741 acgaccagac cgcccttggc ctccagttcg agggagtcgg gaccgagttc cctgcccgcc
     7801 tcgtcccaca gggtgcggct caactgggcc agggccagct cggggccggg ttccttgtcg
     7861 gagaggagga cgttgccctc gccgcggctc gtcgtcccgg cgcccaccgg accgcggtcg
     7921 acgaccgtga cgtccaggcc cgccgaggcg gcgtggaagg cgcaggcggc tccgacgact
     7981 cccgcgccca cgacgacgat cttcagggtc aacggccctc ctccgagcgc cacgggccaa
     8041 cagcgtcgat caccagcacc cgcgctgtgc aatgtcacat gccatgagtg aggctataca
     8101 tcccgccgtt ggaagacaac gccctcttcg tgcccgggag tcagaggtcg actcgggtgc
     8161 ccaggccggt cagggcgccg accgggtcta ggtccggggt accgcgcggc caccagtcct
     8221 cgcggccggg ctcggactcg taggcgtacc agaggcctgc ccggccgagt ctgagctgga
     8281 catggccccg ggggtgggtg aggcggttgc gccaggggcg gaaggcgggg aggtcggcgg
     8341 cgagcagcag ggggcgggcg cggtcgaaac ggccggccgg cgggtcccag ggctcctcca
     8401 ggacgtcgag gctctcgggt ccgccctgcc gccaggcggc gacggctcgt gccagctccg
     8461 ccgtgtcgcg tccggcggcc gaggcgaggg aggcgtagag cgcgcgggtg cccgcggtga
     8521 ggccggagcc cgggcgtgcc gccgccagac ggacggcgtc ctgccacagc gtcagcccgc
     8581 cgaccggatc gcgcccggtg gtgagcaggg cgtgggcgcg ggcggcggcg tcggtggcga
     8641 gctggtccag cgcgaacggg tcggggccgc ccggggcggc cgggtaggcc ggcggctgtt
     8701 ccgggtgcgg gggcgcgggc agcggtgcgg ggaggggcgg caggacgcgc cgggcaagga
     8761 cctccccggc gcggacgccg ggcaggggcg ccggtccgcg ctcctgggcg gcgcgggccg
     8821 cgcgggcggc gttgcgccgg gacagggcgt cgagcagcgc gcgttcgccc cggccgcgca
     8881 gcaggaggag cacgaagggg tcggcgtcga gcagacgtgc cgtctggtag cagagggcgg
     8941 cggcgtgttt gcaggggtgc ccggagtcgg ggcagctgca ctgcggggtg aggtcgccgg
     9001 ggccggggag cagtgggacg ccgcggtccg ccaggtcggc cagggagtgg ggcagttcct
     9061 tgtcgaggag ggcggcgatg tgtccgggcc gttcgacggc ggcgtccagg aaccggtccc
     9121 agtgggcgtc gccgagcgtc cgcagccgca cctgcacgcg atacggccgg gcccggctgc
     9181 cctgtacgta ggcgaggacc agcccggggg tcacggtgat ggcgtccacg cgcccccgct
     9241 cggcgtatcc gcgcccacgc tccagtcgcg cggggtccag ggcgccctct tccagcgcgg
     9301 tcacccacgc gttcccccac caggtcccgg cgaggccctc gtcggcggca ttccggggcg
     9361 ggagcgcggg gaaggtgcgc cggagttcgc cgtctcggcc cggggccccc atcgagcggg
     9421 gtgcacgggg tcgcgcgggc ggtgcggagt gggcggcgtc ggcgtcccgg tcacgcccgg
     9481 ccgacggctg ctcggcacgc ctcggccctt ccggccgctc gctgtcggaa gccgagcctt
     9541 cggagcgcgg cgcgtgcccg gcgcgtggag agacggcggc gggctcgctg ccggccccga
     9601 tgaccggacc gacgccgggg ggcggggtgg actcggggga cggagcgggg tcaggggaca
     9661 gagcgggccc ggaggacgga gcgggctcgg gggacaaggc cgccacccga tcctccccgg
     9721 agcccgcagg accggggccg gtgacggggc ctacgccgga cgacgacacc gcctctgggg
     9781 acgagagcgc cgccccttcc tccctggccc ccgcaggacc ggagccggtc gcggggccca
     9841 cggcgaacga cgaggccgcc gctgaagacg aggccgccgc ccccacctcc ccggacccca
     9901 cagaaccgga gccgacggca gaacccacgc cggacgacga ggtcgcctct ggggacggga
     9961 gcgccgcccg tacttcgtgg gaggtccggc cggcgtgctt gtcggcgggc tggctttctc
    10021 cggtctgccc ggccggcgag gtctcctcgg gcgggtccgc ctccgagggg agtcggaagg
    10081 cgtccgcgac gaagtcccgt acggcacgtg cgcgggcgtc ggcggtgccc gtgccgcccg
    10141 tccgccgctg acggccggcc gggcggggcg cccgccgtgc atgtgcggcg cccccggacg
    10201 ccgcggcggc cttcgcctgc cgacgtgccg cccgcagtgc ctcccgcgcc acgtccgcgg
    10261 ggcggctgtc gcctccgtcc gcggcaccgg agcgggactc ttcgacaggg gcggtcgacg
    10321 ggggcaggtc gccggaccgg ccctctcctt cgccccgctg tgccggctct cccggctcca
    10381 ccacttctgc cggattcgat cgatgtgacg gcatgtcagg ggcgtgcccg gggtcgtgcg
    10441 cctccgcatg ccgtgccctg cgcagggcgg cgcgggcggc gtccccggca cgtggctcgg
    10501 tgggctcggt ggtgggctca gtgggcttcc cgcccggggc gccctcctgg ccctgcgccg
    10561 gcgccgacgg tgcggacggt gccggggccg gtgacgccgg cccgtgctcc cggcccgcga
    10621 ccgttccctc gccgcgctcg cgagcctccc gcagggcgcg gcgggcctcg tcggcgggac
    10681 cgggggtcat gacggcctcc gcagggagac gaggtcggac agttcacgtg ccgtcagctc
    10741 ggtgagggcc gactcgccgg agccgaggat cgcgtcggcc agggcccgct tggactggag
    10801 catctcggcg atgcggtcct cgacggtgcc ctcggtgatg agccggtgga cctggaccgg
    10861 ctgggtctgg ccgatgcggt aggcgcggtc ggtcgcctgt tcctccacgg ccgggttcca
    10921 ccagcggtcg aagtgcacga catgaccggc gcgggtgagg ttcaggccgg tgccggcggc
    10981 cttcagggac aggacgagga cgggggtcgc cccgctctgg aagcggtcca ccatccgctc
    11041 gcgctccggc accggcgttc cgccgtgcag gaggtcgacc gggaccgcgc gggcgcccag
    11101 gtgggaggtg atgaggcggg ccatgccgac gtactgcgtg aagaccagca ccgagccgtc
    11161 ctcggcgagc accgtgtcca gcagctcgtc cagcagggcg agcttgccgg atcgggcggt
    11221 catccggtcg gccccgcccg gcgggtgttc ctccttcagg aacagcgcgg ggtggtcgca
    11281 gatctgcttc agcgaggtca gcagcttgag gacgaggccg cggcggccca tgccctcggc
    11341 ctcctcgatg gcgagcatcg actcgcgcac caccgcctcg tagagcgcgg cctgttcgcg
    11401 ggtgagcggg acggggtggt ccgtctcggt cttcggcggc agctcgggga cgatgcccgg
    11461 gtcggacttc ctgcggcgca gcaggaaggg gcggatgagg cgggcgaggc gctccaccgc
    11521 ctggtcgtcc tcgccgttct ccaccgcgcg cgcgtgccgg gcgcggaagg acttcagggg
    11581 gccgagcagc ccgggggtgg tccagtcgag cagcgcccac agctcggaga ggttgttctc
    11641 cacgggggtg ccggtcagcg ccacccgcgc cggggacggg atcgtgcgca gggccttcgc
    11701 cgtcgccgag tacgggttct tcacgtgctg ggcctcgtcc gcgacgacca tgccccaggg
    11761 gtgctcggcc agggtcgtgg cggcggatcg catcgtgccg taggtggtga ggacgaagcc
    11821 gccggtcagg tcgtcgaggg tgcggtcggg gccgtggaag cggcggacgg ggacgccggg
    11881 cgcgaagcgc tcgatctccc gctgccagtt gcccaggagg gaggccgggc agacgacgag
    11941 ggtcggttcg gtgcgggccc gcttcagatg cagggcgatg acggtgacgg tcttgccgag
    12001 gcccatgtcg tcggcgaggc agccgccgag gccgagcgag gtcatgaggt cgagccagcc
    12061 caggccgcgc agttggtagt cgcggagggt ggcgtgcagg ccgggagggg gtgcggcggg
    12121 gcgcaccccg gcggtgagcc ggtcgcgcag ggcggccagc gcgccgacgg gcaccacgtc
    12181 gaccgtttcg ccgtcggcct cggcgctgcc ggtgagggcg acggagaggg cgtcgaccgg
    12241 gtcgagcagg cccaggtcgc gtttacgggc gcggcggacg agcgaggggt cgaccagcac
    12301 ccaccggtcg cggagccgga cgaccgggcg gtgggcctcc gccagggtgt ccatctcggc
    12361 ctcggtgagc gggtcgccgc cgatcgcgag ctgccagcgg aactggagca ggtcctcgct
    12421 ctcgaagaag cccgtgccgt cggtcgccga accgggcgcg gtcctgacca cggcggtcgc
    12481 ggtgaggtcc tgggccaggt cgcggggcca gtgcacggcg acgcccgcgg cggcgagccg
    12541 gccggcagcc acgcccagca ggtcggtgac ctcctcctcg gacagggcga ggacgtcggg
    12601 gacgtcctgt tcggtgagcc ggtccagcgg cggccagacc cgcgccgcgc ggcgcaccgc
    12661 cagggcggcg tccacgcgcg cgcgggcgcc gaacgacgcg tctgccgccc ccgcccacag
    12721 gtccgctgcg tcggcgacga gcgtggggtc ggcgaggctg tgcacctgga cgatcgccgc
    12781 gcccgcgttg cgcacgcccg cgctgccgcc gccttccgtg ccgcccgcgc ccccgtccgc
    12841 ctcgcggtcg aacaggtcgt acgccgacag gtcgaggcgg agcgagatcc gcacgcccgc
    12901 gtccatgccc gcggcgacct cggcggccca gtcgtgggcg tcgggcaggt gctgggcccc
    12961 gcgggcggcg aacggacggc cggaggcgtg ggacgcggcc ggcgtgcgcg gg
//
